Prupe.2G089200_v2.0.a1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0001235
Physical Location & Seq
Reverse (-)
14081815 .. 14090286
8472 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G089200.1

Sequence Viewer

Length: 1413 bp
ATGGCTCTAGAGAAGACTAGATTCGAAACCATAACCCCATCTCGTTTCATCACCTTCACTTTCCCCAACCCCTCCAACTCAACCACCCTCCTCCGCGTCGCCGTCCTCGACACACCTTTTCAACTCACCGGCTCACCCCGAGTAGCCGCCATGCTCGTCCCCAAACAACGGGAGTCCGACTGGATCTTCTCCACCGAGTCCGGTCACCTCCAGCTCCTGCTCAGCTCTCCCGGGATCGCCCGCCTAATCCTCACCGGCAACCAGCCCAGCAACGGTCACCATTCACGGGCAATCTACCACCGTCTAGCTAGAAACGACTCGTCGTGTGATGGCGAACTCGGGGTGAGTTTGAAACCTCTTTTCCTTGCTTTATCTCCTAAGTCTTGCTTCAAACATGGCATTCCTGAGATACCCATTTTGAGTTATGAGGATAATGTGATTTGTGGTGTGGTTTTGGAGAGGTGTGTTGGGTCTTTGGTTGGTGAAATGGTGGTGGAAGATGTGGAGATTGAGAGTGGTGGTGAGGCTTCAAAGAGGGAGTTCAGGAGGCGTTTGAGGTTTAAAAGAATGCCCAATTTGGTTCAAACGGAGGTACGTATTGTTCCCAACATGGGTTTTGGTTTGGATTATGTGGAAATCGGAGAAGTAGAGTTTAGGCTGGATAATAGTATTTTAGTGCACCCTTATTTGGTTCCTATGGTGGCAAGTCTTCAGTTGATTGCTTCTTATATTGAGGGTCGGATTCGAAGTGGGTTTAGGCCGAAAGCTTTGTGTTTAGGAGTTGGGGGTGGAGCTTTGCTTGGATTTTTAAAAGCCGAACTGGGTTTTCAGGTTGTGGGTGTGGAGGCGGACAAGGAAGTTTTAAGAGTTGCTAGGAAGTATTTTGGACTGGAAGATGGTGGCGGTGAGCATATCAATGTTTGCGTTGGAGATGCAATAAAAGTTATAGAGAAACTTGCGGGTCGAGGTAATGGACAAAGTTCTGGTTCTGTTGGTGCTCATGAGATAGTGGATGATTGTGCTGTGCTCGATGGCAATGACGTTGATTCTAAATTTAATGTAGTGCTAGTTGATTTAGATTCAAGTGATGCTGGAGATGGTATAATTGCTCCACCATTCGAGTTTGTTAGGAACGATGTTCTTTTGGCTGCCAGATCAGTTCTTTGTGATAATGGGATCCTTGCTATAAATGTTATTCCTCCAAATAGATCATTTTACACAACATTGATACAGGAGTTCAGAGAAGTTTTCCACGAGTTGTATGAAATCGATGTTGGAAATGGGGAAAATTTTATTCTTATTGCTCTTGTGTCCCCTGTTACATCTTCTACCAGTGATTGCGAGGATTGCTTCCTTAACAAACTAAGAACGGTTATATCAGGAGCATACATGAATTCTATAAAGAAGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synth

Protein Analysis

471

Amino Acids

51.33

Weight (kDa)

5.36

Isoelectric Point (pI)

42.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 96
AciI CCGC 6 cut(s) 94, 147, 241, 848, 903, 959
AclWI GGATC 4 cut(s) 191, 242, 1171, 1184
AcsI RAATTY 3 cut(s) 1052, 1288, 1393
AcuI CTGAAG 1 cut(s) 695
AfaI GTAC 1 cut(s) 594
AfiI CCNNNNNNNGG 5 cut(s) 168, 272, 286, 611, 688
AgsI TTSAA 6 cut(s) 122, 352, 391, 531, 584, 1083
AjuI GAANNNNNNNTTGG 2 cut(s) 1257, 1289
AluBI AGCT 5 cut(s) 214, 225, 308, 767, 794
AluI AGCT 5 cut(s) 214, 225, 308, 767, 794
Alw21I GWGCWC 3 cut(s) 681, 1000, 1029
Alw44I GTGCAC 1 cut(s) 677
AlwI GGATC 4 cut(s) 191, 242, 1171, 1184
AlwNI CAGNNNCTG 1 cut(s) 217
Ama87I CYCGRG 3 cut(s) 138, 230, 338
AoxI GGCC 1 cut(s) 758
ApaLI GTGCAC 1 cut(s) 677
ApeKI GCWGC 1 cut(s) 1148
ApoI RAATTY 3 cut(s) 1052, 1288, 1393
AsuC2I CCSGG 2 cut(s) 231, 232
AsuII TTCGAA 2 cut(s) 24, 745
AvaI CYCGRG 3 cut(s) 138, 230, 338
BaeGI GKGCMC 1 cut(s) 681
BaeI ACNNNNGTAYC 2 cut(s) 1220, 1253
BamHI GGATCC 1 cut(s) 1176
BauI CACGAG 1 cut(s) 1253
BbsI GAAGAC 2 cut(s) 20, 701
Bbv12I GWGCWC 3 cut(s) 681, 1000, 1029
BbvI GCAGC 1 cut(s) 1135
BccI CCATC 5 cut(s) 46, 323, 890, 1025, 1091
BceAI ACGGC 1 cut(s) 86
BcnI CCSGG 2 cut(s) 231, 232
BfaI CTAG 6 cut(s) 8, 18, 305, 309, 873, 1067
BglII AGATCT 1 cut(s) 1407
BisI GCNGC 2 cut(s) 147, 1149
BlpI GCTNAGC 1 cut(s) 221
BlsI GCNGC 2 cut(s) 148, 1150
Bme1390I CCNGG 2 cut(s) 231, 232
BmeT110I CYCGRG 3 cut(s) 138, 230, 338
BmiI GGNNCC 2 cut(s) 693, 1178
BmrFI CCNGG 2 cut(s) 231, 232
BmrI ACTGGG 1 cut(s) 830
BmsI GCATC 2 cut(s) 922, 1078
BmuI ACTGGG 1 cut(s) 830
BpiI GAAGAC 2 cut(s) 20, 701
BpmI CTGGAG 2 cut(s) 194, 1113
Bpu1102I GCTNAGC 1 cut(s) 221
Bpu14I TTCGAA 2 cut(s) 24, 745
BpuMI CCSGG 2 cut(s) 231, 232
Bsa29I ATCGAT 1 cut(s) 1269
BsaAI YACGTR 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 230
BsaWI WCCGGW 1 cut(s) 200
Bsc4I CCNNNNNNNGG 5 cut(s) 168, 272, 286, 611, 688
Bse118I RCCGGY 2 cut(s) 128, 254
Bse1I ACTGG 4 cut(s) 185, 825, 894, 1332
Bse3DI GCAATG 1 cut(s) 1042
BseCI ATCGAT 1 cut(s) 1269
BseDI CCNNGG 1 cut(s) 230
BseGI GGATG 1 cut(s) 1018
BseLI CCNNNNNNNGG 5 cut(s) 168, 272, 286, 611, 688
BseMI GCAATG 1 cut(s) 1042
BseMII CTCAG 2 cut(s) 235, 396
BseNI ACTGG 4 cut(s) 185, 825, 894, 1332
BseRI GAGGAG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 681
BseXI GCAGC 1 cut(s) 1135
BseYI CCCAGC 1 cut(s) 266
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 1 cut(s) 760
BshVI ATCGAT 1 cut(s) 1269
BsiHKAI GWGCWC 3 cut(s) 681, 1000, 1029
BsiHKCI CYCGRG 3 cut(s) 138, 230, 338
BsiSI CCGG 4 cut(s) 129, 201, 231, 255
BslFI GGGAC 2 cut(s) 143, 1297
BslI CCNNNNNNNGG 5 cut(s) 168, 272, 286, 611, 688
BsmFI GGGAC 2 cut(s) 143, 1297
BsmI GAATGC 2 cut(s) 399, 573
BsnI GGCC 1 cut(s) 760
BsoBI CYCGRG 3 cut(s) 138, 230, 338
Bsp119I TTCGAA 2 cut(s) 24, 745
Bsp1286I GDGCHC 3 cut(s) 681, 1000, 1029
Bsp143I GATC 6 cut(s) 183, 234, 1154, 1176, 1208, 1407
Bsp1720I GCTNAGC 1 cut(s) 221
BspACI CCGC 6 cut(s) 94, 147, 241, 848, 903, 959
BspANI GGCC 1 cut(s) 760
BspCNI CTCAG 2 cut(s) 234, 397
BspDI ATCGAT 1 cut(s) 1269
BspFNI CGCG 1 cut(s) 96
BspHI TCATGA 1 cut(s) 1000
BspLI GGNNCC 2 cut(s) 693, 1178
BspPI GGATC 4 cut(s) 191, 242, 1171, 1184
BspT104I TTCGAA 2 cut(s) 24, 745
BsrDI GCAATG 1 cut(s) 1042
BsrFI RCCGGY 2 cut(s) 128, 254
BsrI ACTGG 4 cut(s) 185, 825, 894, 1332
BssAI RCCGGY 2 cut(s) 128, 254
BssECI CCNNGG 1 cut(s) 230
BssMI GATC 6 cut(s) 183, 234, 1154, 1176, 1208, 1407
BssSI CACGAG 1 cut(s) 1253
Bst2BI CACGAG 1 cut(s) 1253
Bst4CI ACNGT 3 cut(s) 275, 302, 1372
BstBAI YACGTR 1 cut(s) 596
BstBI TTCGAA 2 cut(s) 24, 745
BstC8I GCNNGC 1 cut(s) 241
BstDEI CTNAG 4 cut(s) 221, 378, 405, 1364
BstEII GGTNACC 2 cut(s) 203, 275
BstF5I GGATG 1 cut(s) 1018
BstFNI CGCG 1 cut(s) 96
BstKTI GATC 6 cut(s) 186, 237, 1157, 1179, 1211, 1410
BstMBI GATC 6 cut(s) 183, 234, 1154, 1176, 1208, 1407
BstMWI GCNNNNNNNGC 1 cut(s) 1347
BstPI GGTNACC 2 cut(s) 203, 275
BstSCI CCNGG 2 cut(s) 229, 230
BstSLI GKGCMC 1 cut(s) 681
BstSNI TACGTA 1 cut(s) 596
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 1135
BstV2I GAAGAC 2 cut(s) 20, 701
BstX2I RGATCY 3 cut(s) 183, 1176, 1407
BstYI RGATCY 3 cut(s) 183, 1176, 1407
Bsu15I ATCGAT 1 cut(s) 1269
BsuRI GGCC 1 cut(s) 760
BsuTUI ATCGAT 1 cut(s) 1269
BtsCI GGATG 1 cut(s) 1018
BtsIMutI CAGTG 1 cut(s) 1339
Cac8I GCNNGC 1 cut(s) 241
CaiI CAGNNNCTG 1 cut(s) 217
CciI TCATGA 1 cut(s) 1000
Cfr10I RCCGGY 2 cut(s) 128, 254
Cfr9I CCCGGG 1 cut(s) 230
ClaI ATCGAT 1 cut(s) 1269
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 593
CviAII CATG 5 cut(s) 151, 395, 610, 1001, 1390
CviQI GTAC 1 cut(s) 593
DdeI CTNAG 4 cut(s) 221, 378, 405, 1364
DpnI GATC 6 cut(s) 185, 236, 1156, 1178, 1210, 1409
DpnII GATC 6 cut(s) 183, 234, 1154, 1176, 1208, 1407
DraI TTTAAA 2 cut(s) 562, 810
EciI GGCGGA 1 cut(s) 863
Eco105I TACGTA 1 cut(s) 596
Eco57I CTGAAG 1 cut(s) 695
Eco88I CYCGRG 3 cut(s) 138, 230, 338
Eco91I GGTNACC 2 cut(s) 203, 275
EcoO65I GGTNACC 2 cut(s) 203, 275
EcoRI GAATTC 1 cut(s) 1393
FaeI CATG 5 cut(s) 154, 398, 613, 1004, 1393
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 2 cut(s) 143, 1297
FatI CATG 5 cut(s) 150, 394, 609, 1000, 1389
FauI CCCGC 2 cut(s) 248, 952
Fnu4HI GCNGC 2 cut(s) 147, 1149
FokI GGATG 1 cut(s) 1025
Fsp4HI GCNGC 2 cut(s) 147, 1149
FspBI CTAG 6 cut(s) 8, 18, 305, 309, 873, 1067
GluI GCNGC 2 cut(s) 147, 1149
GsaI CCCAGC 1 cut(s) 270
GsuI CTGGAG 2 cut(s) 194, 1113
HaeIII GGCC 1 cut(s) 760
HapII CCGG 4 cut(s) 129, 201, 231, 255
HgaI GACGC 1 cut(s) 85
Hin1II CATG 5 cut(s) 154, 398, 613, 1004, 1393
HindIII AAGCTT 1 cut(s) 765
HinfI GANTC 7 cut(s) 21, 173, 197, 317, 742, 1046, 1079
HpaII CCGG 4 cut(s) 129, 201, 231, 255
Hpy166II GTNNAC 1 cut(s) 679
Hpy188I TCNGA 5 cut(s) 178, 641, 741, 1241, 1412
Hpy188III TCNNGA 5 cut(s) 8, 404, 544, 1001, 1380
Hpy8I GTNNAC 1 cut(s) 679
Hpy99I CGWCG 2 cut(s) 101, 325
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 3 cut(s) 275, 302, 1372
HpyCH4IV ACGT 2 cut(s) 595, 1041
HpyCH4V TGCA 2 cut(s) 679, 935
HpyF10VI GCNNNNNNNGC 1 cut(s) 1347
HpyF3I CTNAG 4 cut(s) 221, 378, 405, 1364
HpySE526I ACGT 2 cut(s) 595, 1041
Hsp92II CATG 5 cut(s) 154, 398, 613, 1004, 1393
Kzo9I GATC 6 cut(s) 183, 234, 1154, 1176, 1208, 1407
LmnI GCTCC 4 cut(s) 219, 791, 1114, 1382
Lsp1109I GCAGC 1 cut(s) 1135
LweI GCATC 2 cut(s) 922, 1078
MaeI CTAG 6 cut(s) 8, 18, 305, 309, 873, 1067
MaeII ACGT 2 cut(s) 595, 1041
MaeIII GTNAC 3 cut(s) 203, 275, 1318
MalI GATC 6 cut(s) 185, 236, 1156, 1178, 1210, 1409
MboI GATC 6 cut(s) 183, 234, 1154, 1176, 1208, 1407
MboII GAAGA 6 cut(s) 25, 178, 509, 701, 905, 1317
MflI RGATCY 3 cut(s) 183, 1176, 1407
MhlI GDGCHC 3 cut(s) 681, 1000, 1029
MluCI AATT 5 cut(s) 574, 1052, 1104, 1288, 1393
MlyI GAGTC 3 cut(s) 182, 206, 311
MmeI TCCRAC 5 cut(s) 99, 201, 719, 907, 1255
MseI TTAA 5 cut(s) 561, 809, 863, 1056, 1356
MslI CAYNNNNRTG 1 cut(s) 915
MspI CCGG 4 cut(s) 129, 201, 231, 255
MspR9I CCNGG 2 cut(s) 231, 232
Mva1269I GAATGC 2 cut(s) 399, 573
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 1 cut(s) 1347
NciI CCSGG 2 cut(s) 231, 232
NdeII GATC 6 cut(s) 183, 234, 1154, 1176, 1208, 1407
NlaIII CATG 5 cut(s) 154, 398, 613, 1004, 1393
NlaIV GGNNCC 2 cut(s) 693, 1178
NmuCI GTSAC 2 cut(s) 203, 275
NspV TTCGAA 2 cut(s) 24, 745
PagI TCATGA 1 cut(s) 1000
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 2 cut(s) 399, 573
PfeI GAWTC 4 cut(s) 21, 742, 1046, 1079
PkrI GCNGC 2 cut(s) 148, 1150
PleI GAGTC 3 cut(s) 181, 205, 311
PpsI GAGTC 3 cut(s) 181, 205, 311
Ppu21I YACGTR 1 cut(s) 596
PspEI GGTNACC 2 cut(s) 203, 275
PspFI CCCAGC 1 cut(s) 266
PspN4I GGNNCC 2 cut(s) 693, 1178
PsrI GAACNNNNNNTAC 2 cut(s) 585, 617
PstNI CAGNNNCTG 1 cut(s) 217
PsuI RGATCY 3 cut(s) 183, 1176, 1407
RsaI GTAC 1 cut(s) 594
RsaNI GTAC 1 cut(s) 593
RseI CAYNNNNRTG 1 cut(s) 915
SaqAI TTAA 5 cut(s) 561, 809, 863, 1056, 1356
SatI GCNGC 2 cut(s) 147, 1149
Sau3AI GATC 6 cut(s) 183, 234, 1154, 1176, 1208, 1407
SchI GAGTC 3 cut(s) 182, 206, 311
ScrFI CCNGG 2 cut(s) 231, 232
SduI GDGCHC 3 cut(s) 681, 1000, 1029
SfaNI GCATC 2 cut(s) 922, 1078
SfuI TTCGAA 2 cut(s) 24, 745
SmaI CCCGGG 1 cut(s) 232
SmiMI CAYNNNNRTG 1 cut(s) 915
SnaBI TACGTA 1 cut(s) 596
Sse9I AATT 5 cut(s) 574, 1052, 1104, 1288, 1393
SsiI CCGC 6 cut(s) 94, 147, 241, 848, 903, 959
SspMI CTAG 6 cut(s) 8, 18, 305, 309, 873, 1067
StyD4I CCNGG 2 cut(s) 229, 230
TaaI ACNGT 3 cut(s) 275, 302, 1372
TaiI ACGT 2 cut(s) 598, 1044
TaqI TCGA 7 cut(s) 24, 108, 745, 964, 1029, 1119, 1269
TasI AATT 5 cut(s) 574, 1052, 1104, 1288, 1393
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 4 cut(s) 21, 742, 1046, 1079
Tru1I TTAA 5 cut(s) 561, 809, 863, 1056, 1356
Tru9I TTAA 5 cut(s) 561, 809, 863, 1056, 1356
TscAI CASTG 1 cut(s) 1339
TseFI GTSAC 2 cut(s) 203, 275
TseI GCWGC 1 cut(s) 1148
Tsp45I GTSAC 2 cut(s) 203, 275
TspDTI ATGAA 3 cut(s) 37, 1278, 1406
TspGWI ACGGA 1 cut(s) 602
TspMI CCCGGG 1 cut(s) 230
TspRI CASTG 1 cut(s) 1339
VneI GTGCAC 1 cut(s) 677
XapI RAATTY 3 cut(s) 1052, 1288, 1393
XbaI TCTAGA 1 cut(s) 7
XmaI CCCGGG 1 cut(s) 230
XspI CTAG 6 cut(s) 8, 18, 305, 309, 873, 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.