Rorug01G0129700

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001235
Physical Location & Seq
Forward (+)
22036537 .. 22045601
9065 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0129700.1

Sequence Viewer

Length: 564 bp
ATGGCAGTGATGGCAGATTTTGGTTTGCTTGTTCTGTTCTTCATTGCGTTGTGGGACGCTGAGGCGGTGTTGAGTTCAGGGGTTGCAGGCGTTGTTGTTTCTGTGCCCCAGCTCGACGGTGACATGAATGATGCGGCCTCCGGGAGGGAGTTGGCATCTAAATGGCAACTGAGCAGCGATCCGGTCTTGGTTTGGTCAAGATCGGATCGGAAAGGTCGATTCCGATCGAGTCCATGGGATGATGTTCTCTGGCGTGCAGGCGATCTTGATCTCCCGGACGGTGACAGAGCGCGGGTTTGCTCGGACGCAGGGACTATTGCTTCTGCTGGTGGTGCTCATCGATCACAGGTCCAACTAAATAAGTTGGGAGATCAGCTTGGTTTAGATGCTTATGGAATTCATGCCAATGAAGAGGATTCAAGCATTAATATTGTCAGCGACAGGGAAACTCATTTTCCAGTCCCCTTAAATAAGGAGCAGCTGAATGATGTTTCTTCGAGCAAGAAAAGGCTCGATGTAAATAGGAATTCTGCCAAGGAACCAAAACGAGGTGGTGTGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synth

Protein Analysis

187

Amino Acids

20.16

Weight (kDa)

5.04

Isoelectric Point (pI)

47.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 292
AciI CCGC 3 cut(s) 65, 134, 292
AclWI GGATC 2 cut(s) 173, 213
AcsI RAATTY 2 cut(s) 396, 526
AfiI CCNNNNNNNGG 2 cut(s) 144, 548
AgsI TTSAA 1 cut(s) 420
AluBI AGCT 3 cut(s) 112, 376, 481
AluI AGCT 3 cut(s) 112, 376, 481
Alw21I GWGCWC 1 cut(s) 337
AlwI GGATC 2 cut(s) 173, 213
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 2 cut(s) 174, 478
ApoI RAATTY 2 cut(s) 396, 526
AseI ATTAAT 1 cut(s) 426
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 1 cut(s) 349
AsuC2I CCSGG 2 cut(s) 142, 275
AsuHPI GGTGA 2 cut(s) 131, 293
AvaII GGWCC 1 cut(s) 349
BaeGI GKGCMC 1 cut(s) 108
Bbv12I GWGCWC 1 cut(s) 337
BbvCI CCTCAGC 1 cut(s) 60
BbvI GCAGC 2 cut(s) 186, 490
BccI CCATC 1 cut(s) 4
BcnI CCSGG 2 cut(s) 142, 275
BisI GCNGC 3 cut(s) 135, 175, 479
BlsI GCNGC 3 cut(s) 136, 176, 480
Bme1390I CCNGG 2 cut(s) 142, 275
Bme18I GGWCC 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 349
BmiI GGNNCC 1 cut(s) 540
BmrFI CCNGG 2 cut(s) 142, 275
BmsI GCATC 3 cut(s) 121, 164, 376
Bpu10I CCTNAGC 1 cut(s) 60
BpuMI CCSGG 2 cut(s) 142, 275
Bsa29I ATCGAT 1 cut(s) 340
BsaBI GATNNNNATC 2 cut(s) 223, 267
BsaJI CCNNGG 2 cut(s) 233, 534
BsaWI WCCGGW 1 cut(s) 181
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 548
Bse1I ACTGG 1 cut(s) 458
Bse3DI GCAATG 1 cut(s) 42
Bse8I GATNNNNATC 2 cut(s) 223, 267
BseCI ATCGAT 1 cut(s) 340
BseDI CCNNGG 2 cut(s) 233, 534
BseGI GGATG 1 cut(s) 244
BseJI GATNNNNATC 2 cut(s) 223, 267
BseLI CCNNNNNNNGG 2 cut(s) 144, 548
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 2 cut(s) 51, 161
BseNI ACTGG 1 cut(s) 458
BseSI GKGCMC 1 cut(s) 108
BseXI GCAGC 2 cut(s) 186, 490
BseYI CCCAGC 1 cut(s) 108
BsgI GTGCAG 1 cut(s) 276
Bsh1236I CGCG 1 cut(s) 292
Bsh1285I CGRYCG 1 cut(s) 227
BshFI GGCC 1 cut(s) 137
BshVI ATCGAT 1 cut(s) 340
BsiEI CGRYCG 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 3 cut(s) 141, 182, 275
BslFI GGGAC 3 cut(s) 68, 325, 446
BslI CCNNNNNNNGG 2 cut(s) 144, 548
BsmFI GGGAC 3 cut(s) 68, 325, 446
BsnI GGCC 1 cut(s) 137
Bsp1286I GDGCHC 2 cut(s) 108, 337
Bsp143I GATC 8 cut(s) 178, 200, 205, 224, 262, 268, 341, 370
Bsp19I CCATGG 1 cut(s) 233
BspACI CCGC 3 cut(s) 65, 134, 292
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 2 cut(s) 52, 162
BspDI ATCGAT 1 cut(s) 340
BspFNI CGCG 1 cut(s) 292
BspLI GGNNCC 1 cut(s) 540
BspPI GGATC 2 cut(s) 173, 213
BsrDI GCAATG 1 cut(s) 42
BsrI ACTGG 1 cut(s) 458
BssECI CCNNGG 2 cut(s) 233, 534
BssMI GATC 8 cut(s) 178, 200, 205, 224, 262, 268, 341, 370
BssT1I CCWWGG 2 cut(s) 233, 534
Bst4CI ACNGT 2 cut(s) 119, 281
Bst6I CTCTTC 1 cut(s) 405
BstC8I GCNNGC 3 cut(s) 88, 255, 259
BstDEI CTNAG 2 cut(s) 60, 170
BstDSI CCRYGG 1 cut(s) 233
BstENI CCTNNNNNAGG 1 cut(s) 142
BstF5I GGATG 1 cut(s) 244
BstFNI CGCG 1 cut(s) 292
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 8 cut(s) 181, 203, 208, 227, 265, 271, 344, 373
BstMBI GATC 8 cut(s) 178, 200, 205, 224, 262, 268, 341, 370
BstMCI CGRYCG 1 cut(s) 227
BstMWI GCNNNNNNNGC 2 cut(s) 11, 332
BstSCI CCNGG 2 cut(s) 140, 273
BstSLI GKGCMC 1 cut(s) 108
BstUI CGCG 1 cut(s) 292
BstV1I GCAGC 2 cut(s) 186, 490
Bsu15I ATCGAT 1 cut(s) 340
BsuRI GGCC 1 cut(s) 137
BsuTUI ATCGAT 1 cut(s) 340
BtgI CCRYGG 1 cut(s) 233
BtsCI GGATG 1 cut(s) 244
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 1 cut(s) 12
Cac8I GCNNGC 3 cut(s) 88, 255, 259
CfoI GCGC 1 cut(s) 292
Cfr13I GGNCC 1 cut(s) 349
ClaI ATCGAT 1 cut(s) 340
CseI GACGC 2 cut(s) 65, 314
CviAII CATG 3 cut(s) 124, 234, 401
CviJI RGCY 5 cut(s) 112, 137, 376, 481, 511
CviKI_1 RGCY 5 cut(s) 112, 137, 376, 481, 511
DdeI CTNAG 2 cut(s) 60, 170
DpnI GATC 8 cut(s) 180, 202, 207, 226, 264, 270, 343, 372
DpnII GATC 8 cut(s) 178, 200, 205, 224, 262, 268, 341, 370
Eam1104I CTCTTC 1 cut(s) 405
EarI CTCTTC 1 cut(s) 405
Eco130I CCWWGG 2 cut(s) 233, 534
Eco47I GGWCC 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 142
EcoRI GAATTC 2 cut(s) 396, 526
EcoT14I CCWWGG 2 cut(s) 233, 534
ErhI CCWWGG 2 cut(s) 233, 534
FaeI CATG 3 cut(s) 127, 237, 404
FaiI YATR 4 cut(s) 125, 235, 393, 402
FaqI GGGAC 3 cut(s) 68, 325, 446
FatI CATG 3 cut(s) 123, 233, 400
FauI CCCGC 1 cut(s) 285
Fnu4HI GCNGC 3 cut(s) 135, 175, 479
FokI GGATG 1 cut(s) 251
Fsp4HI GCNGC 3 cut(s) 135, 175, 479
GlaI GCGC 1 cut(s) 291
GluI GCNGC 3 cut(s) 135, 175, 479
GsaI CCCAGC 1 cut(s) 112
HaeIII GGCC 1 cut(s) 137
HapII CCGG 3 cut(s) 141, 182, 275
HgaI GACGC 2 cut(s) 65, 314
HhaI GCGC 1 cut(s) 292
Hin1II CATG 3 cut(s) 127, 237, 404
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HinfI GANTC 3 cut(s) 219, 229, 416
HpaII CCGG 3 cut(s) 141, 182, 275
HphI GGTGA 2 cut(s) 131, 293
Hpy188I TCNGA 4 cut(s) 205, 210, 224, 304
Hpy188III TCNNGA 2 cut(s) 198, 266
Hpy99I CGWCG 1 cut(s) 119
HpyCH4III ACNGT 2 cut(s) 119, 281
HpyCH4V TGCA 2 cut(s) 86, 257
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 332
HpyF3I CTNAG 2 cut(s) 60, 170
Hsp92II CATG 3 cut(s) 127, 237, 404
HspAI GCGC 1 cut(s) 290
Kzo9I GATC 8 cut(s) 178, 200, 205, 224, 262, 268, 341, 370
LmnI GCTCC 1 cut(s) 475
Lsp1109I GCAGC 2 cut(s) 186, 490
LweI GCATC 3 cut(s) 121, 164, 376
MaeIII GTNAC 2 cut(s) 119, 281
MalI GATC 8 cut(s) 180, 202, 207, 226, 264, 270, 343, 372
MboI GATC 8 cut(s) 178, 200, 205, 224, 262, 268, 341, 370
MboII GAAGA 3 cut(s) 31, 422, 486
MhlI GDGCHC 2 cut(s) 108, 337
MluCI AATT 2 cut(s) 396, 526
MlyI GAGTC 1 cut(s) 238
MmeI TCCRAC 1 cut(s) 376
MnlI CCTC 5 cut(s) 55, 138, 148, 406, 542
MseI TTAA 2 cut(s) 426, 467
MslI CAYNNNNRTG 2 cut(s) 160, 405
MspA1I CMGCKG 1 cut(s) 481
MspI CCGG 3 cut(s) 141, 182, 275
MspR9I CCNGG 2 cut(s) 142, 275
MvnI CGCG 1 cut(s) 292
MwoI GCNNNNNNNGC 2 cut(s) 11, 332
NciI CCSGG 2 cut(s) 142, 275
NcoI CCATGG 1 cut(s) 233
NdeII GATC 8 cut(s) 178, 200, 205, 224, 262, 268, 341, 370
NlaIII CATG 3 cut(s) 127, 237, 404
NlaIV GGNNCC 1 cut(s) 540
NmuCI GTSAC 2 cut(s) 119, 281
PcsI WCGNNNNNNNCGW 1 cut(s) 214
PfeI GAWTC 2 cut(s) 219, 416
PfoI TCCNGGA 2 cut(s) 140, 273
PkrI GCNGC 3 cut(s) 136, 176, 480
Ple19I CGATCG 1 cut(s) 227
PleI GAGTC 1 cut(s) 237
PpsI GAGTC 1 cut(s) 237
PshBI ATTAAT 1 cut(s) 426
PspFI CCCAGC 1 cut(s) 108
PspN4I GGNNCC 1 cut(s) 540
PspPI GGNCC 1 cut(s) 349
PvuI CGATCG 1 cut(s) 227
PvuII CAGCTG 1 cut(s) 481
RseI CAYNNNNRTG 2 cut(s) 160, 405
SaqAI TTAA 2 cut(s) 426, 467
SatI GCNGC 3 cut(s) 135, 175, 479
Sau3AI GATC 8 cut(s) 178, 200, 205, 224, 262, 268, 341, 370
Sau96I GGNCC 1 cut(s) 349
SchI GAGTC 1 cut(s) 238
ScrFI CCNGG 2 cut(s) 142, 275
SduI GDGCHC 2 cut(s) 108, 337
SetI ASST 6 cut(s) 114, 217, 351, 378, 483, 553
SfaNI GCATC 3 cut(s) 121, 164, 376
SinI GGWCC 1 cut(s) 349
SmiMI CAYNNNNRTG 2 cut(s) 160, 405
Sse9I AATT 2 cut(s) 396, 526
SsiI CCGC 3 cut(s) 65, 134, 292
SspI AATATT 1 cut(s) 430
StyD4I CCNGG 2 cut(s) 140, 273
StyI CCWWGG 2 cut(s) 233, 534
TaaI ACNGT 2 cut(s) 119, 281
TaqI TCGA 6 cut(s) 114, 217, 227, 340, 497, 513
TasI AATT 2 cut(s) 396, 526
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 2 cut(s) 219, 416
Tru1I TTAA 2 cut(s) 426, 467
Tru9I TTAA 2 cut(s) 426, 467
TscAI CASTG 1 cut(s) 12
TseFI GTSAC 2 cut(s) 119, 281
TseI GCWGC 2 cut(s) 174, 478
Tsp45I GTSAC 2 cut(s) 119, 281
TspDTI ATGAA 4 cut(s) 31, 140, 389, 423
TspRI CASTG 1 cut(s) 12
VpaK11BI GGWCC 1 cut(s) 349
VspI ATTAAT 1 cut(s) 426
XagI CCTNNNNNAGG 1 cut(s) 142
XapI RAATTY 2 cut(s) 396, 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.