MD07G1071500.v1.1

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001235
Physical Location & Seq
Reverse (-)
6764600 .. 6768390
3791 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1071500.v1.1.491

Sequence Viewer

Length: 1428 bp
ATGGCTCTGGAGAGGAACACATTCGAAACCATAACCCCATCTCGTTTCATCACCTTCACTTTCCCAAACCCCTCCAACTCCTCCTCACTCCTCCGCGTCGCCGTCCTCGACTCACCTCTTCAACTCACTGACTCACCCCGAGTCGCCGCCATGCTCGTCCCCATCCACCGCGAGTCCGACTGGATATTCTCCACCGAGTCGGGCCACCTCCAGCTCCTTCTCAGCTCGCCTGGGATCTCCCGCCTAATCCTCATCGGCAGCGACCGATCCGTAGACGCTCACCATTCACCGCCAATCTACCACCGTCCGGATAAAAACGGCACGTCGTGTAATGAGGAACTCGGGGTGAGTGAGAAATTGAAACCTCTTTTGCTTGCTTTATCTCCAAAGTGTTGCTTCAAATTTGGCATTCCCGAGATACCCATTTTGTGTTATGAAGATAATGTGGTTTCTAGTGTGGTTTTGGAGAGGTGTGTTGGGTCTCTGGTTGGGGAAATGGTGGTGGAGGATGTGGAGATTGAGAGTGGCGCCGGCGAGGTTTCGAAGAGGGAGTTCAGGAGGCGTTTGAGGTTTAAGAGAATGCCCAATTTGGTTCAAACGGAGGTACGCATTGTTCCGAAGACGGGTTTTGGTTTTGATTGTGTGGAATTTGGAGAAATAGAGTTTAGGCTGGATAATAGTGTTTTAGTGCACCCTTATTTGGTTCCTATGGTGGCAAGTCTGAGTCTTGTTGCTTCTTGTGTCGAGGAACAGATTCGAAGTGGGTTTAGACCCAAAGTTTTGTGTTTAGGAGTTGGTGGTGGAGCTTTGCTTGGATTTTTAAGTGCCCATTTGAGTTTTCAGGTTGTGGGTGTGGAGGCGGATGGGGAGGTTCTAAGGGTTGCTAGGAAGTATTTCGGACTGGAAGATGGTGAGCATATCAATGTTCACGTTGGGGATGCAATAAAGTTTATAGAGAAACTTGCTTTCCGTGTTAATGAACGGACTGCCGGTTCTATTTTTGCAGATGAGGTAGAGGATGGTAGTGAGTCTTGTGACATGGGCGATGGCAATGACGTTCATACTAAATTTGATGTAGTGCTTGCTGATTTAGATTCAAGTGATGCTGGGGATGGTATAATTGCTCCGCCGTTCGAGTTTGTTAGGAAGCATGTGCTTTTGGCTGCCAAATCAGTTCTTTGTGATAATGGCATCTTTGCTATAAATGTTATTCCTCCAAATCGGTCATTTTACACAACAATGATACATGAGTTTAGAGAAGTTTTTCATGAGTTGTATGAAATAGATGTTGGAAATGGGGAAAATTTTATTCTTATTGCTGTTGTGTCGCCTGTTGCATCTGCTACTAGTGATTGTGAAAACTCTTTCCTTAACAAACTAAGAATGGTTGTATCAGGAGCATACTTGAATTCCATAAAGAAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Spermine_synth

Protein Analysis

476

Amino Acids

52.23

Weight (kDa)

5.18

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 527
AccI GTMKAC 1 cut(s) 273
AccII CGCG 2 cut(s) 96, 171
AccIII TCCGGA 1 cut(s) 307
AciI CCGC 7 cut(s) 94, 147, 169, 241, 290, 860, 1127
AclWI GGATC 2 cut(s) 242, 261
AcsI RAATTY 5 cut(s) 401, 647, 1067, 1303, 1408
AcyI GRCGYC 1 cut(s) 528
AdeI CACNNNGTG 1 cut(s) 327
AfaI GTAC 1 cut(s) 606
AfiI CCNNNNNNNGG 3 cut(s) 307, 623, 700
AgsI TTSAA 6 cut(s) 122, 361, 400, 596, 1098, 1408
AhlI ACTAGT 1 cut(s) 1346
AjiI CACGTC 1 cut(s) 324
AjnI CCWGG 1 cut(s) 229
AjuI GAANNNNNNNTTGG 2 cut(s) 1272, 1304
AloI GAACNNNNNNTCC 2 cut(s) 976, 1008
AluBI AGCT 3 cut(s) 214, 225, 806
AluI AGCT 3 cut(s) 214, 225, 806
Alw21I GWGCWC 1 cut(s) 693
Alw26I GTCTC 1 cut(s) 486
Alw44I GTGCAC 1 cut(s) 689
AlwI GGATC 2 cut(s) 242, 261
Ama87I CYCGRG 3 cut(s) 138, 341, 413
Aor13HI TCCGGA 1 cut(s) 307
AoxI GGCC 1 cut(s) 202
ApaLI GTGCAC 1 cut(s) 689
ApeKI GCWGC 2 cut(s) 258, 1163
ApoI RAATTY 5 cut(s) 401, 647, 1067, 1303, 1408
Asp700I GAANNNNTTC 4 cut(s) 20, 753, 893, 1263
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 1 cut(s) 202
AsuHPI GGTGA 7 cut(s) 43, 105, 126, 272, 279, 358, 923
AsuII TTCGAA 3 cut(s) 24, 542, 757
AvaI CYCGRG 3 cut(s) 138, 341, 413
BaeGI GKGCMC 2 cut(s) 693, 829
BaeI ACNNNNGTAYC 2 cut(s) 1235, 1268
BanI GGYRCC 1 cut(s) 527
BbsI GAAGAC 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 693
BbvI GCAGC 2 cut(s) 270, 1150
BccI CCATC 7 cut(s) 46, 170, 857, 902, 1013, 1040, 1106
BceAI ACGGC 3 cut(s) 86, 334, 1114
BciT130I CCWGG 1 cut(s) 231
BcoDI GTCTC 1 cut(s) 486
BcuI ACTAGT 1 cut(s) 1346
BfaI CTAG 3 cut(s) 453, 885, 1347
BfoI RGCGCY 1 cut(s) 531
BglII AGATCT 1 cut(s) 1422
BisI GCNGC 3 cut(s) 147, 259, 1164
BlsI GCNGC 3 cut(s) 148, 260, 1165
Bme1390I CCNGG 1 cut(s) 231
BmeT110I CYCGRG 3 cut(s) 138, 341, 413
BmgBI CACGTC 1 cut(s) 324
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 2 cut(s) 529, 705
BmrFI CCNGG 1 cut(s) 231
BmsI GCATC 4 cut(s) 928, 1093, 1200, 1346
BpiI GAAGAC 1 cut(s) 626
BpmI CTGGAG 2 cut(s) 29, 194
Bpu14I TTCGAA 3 cut(s) 24, 542, 757
BsaHI GRCGYC 1 cut(s) 528
BsaI GGTCTC 1 cut(s) 486
BsaJI CCNNGG 1 cut(s) 230
BsaWI WCCGGW 1 cut(s) 307
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 623, 700
Bse118I RCCGGY 2 cut(s) 530, 989
Bse1I ACTGG 2 cut(s) 185, 906
Bse3DI GCAATG 1 cut(s) 1057
BseAI TCCGGA 1 cut(s) 307
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 230
BseGI GGATG 6 cut(s) 162, 514, 868, 943, 1024, 1117
BseLI CCNNNNNNNGG 3 cut(s) 307, 623, 700
BseMI GCAATG 1 cut(s) 1057
BseMII CTCAG 2 cut(s) 235, 713
BseNI ACTGG 2 cut(s) 185, 906
BseRI GAGGAG 3 cut(s) 70, 73, 80
BseSI GKGCMC 2 cut(s) 693, 829
BseXI GCAGC 2 cut(s) 270, 1150
BseYI CCCAGC 1 cut(s) 1106
Bsh1236I CGCG 2 cut(s) 96, 171
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 1 cut(s) 204
BshNI GGYRCC 1 cut(s) 527
BsiEI CGRYCG 1 cut(s) 265
BsiHKAI GWGCWC 1 cut(s) 693
BsiHKCI CYCGRG 3 cut(s) 138, 341, 413
BsiSI CCGG 3 cut(s) 308, 531, 990
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 3 cut(s) 307, 623, 700
BsmAI GTCTC 1 cut(s) 486
BsmFI GGGAC 1 cut(s) 143
BsmI GAATGC 2 cut(s) 408, 585
BsnI GGCC 1 cut(s) 204
Bso31I GGTCTC 1 cut(s) 486
BsoBI CYCGRG 3 cut(s) 138, 341, 413
Bsp119I TTCGAA 3 cut(s) 24, 542, 757
Bsp1286I GDGCHC 2 cut(s) 693, 829
Bsp13I TCCGGA 1 cut(s) 307
Bsp143I GATC 3 cut(s) 234, 266, 1422
BspACI CCGC 7 cut(s) 94, 147, 169, 241, 290, 860, 1127
BspANI GGCC 1 cut(s) 204
BspCNI CTCAG 2 cut(s) 234, 714
BspEI TCCGGA 1 cut(s) 307
BspFNI CGCG 2 cut(s) 96, 171
BspHI TCATGA 1 cut(s) 1267
BspLI GGNNCC 2 cut(s) 529, 705
BspPI GGATC 2 cut(s) 242, 261
BspT104I TTCGAA 3 cut(s) 24, 542, 757
BspT107I GGYRCC 1 cut(s) 527
BspTNI GGTCTC 1 cut(s) 486
BsrDI GCAATG 1 cut(s) 1057
BsrFI RCCGGY 2 cut(s) 530, 989
BsrI ACTGG 2 cut(s) 185, 906
BssAI RCCGGY 2 cut(s) 530, 989
BssECI CCNNGG 1 cut(s) 230
BssMI GATC 3 cut(s) 234, 266, 1422
BssNI GRCGYC 1 cut(s) 528
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 2 cut(s) 123, 539
BstACI GRCGYC 1 cut(s) 528
BstBI TTCGAA 3 cut(s) 24, 542, 757
BstC8I GCNNGC 4 cut(s) 227, 375, 532, 1083
BstDEI CTNAG 4 cut(s) 221, 722, 875, 1379
BstF5I GGATG 6 cut(s) 162, 514, 868, 943, 1024, 1117
BstFNI CGCG 2 cut(s) 96, 171
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 3 cut(s) 237, 269, 1425
BstMAI GTCTC 1 cut(s) 486
BstMBI GATC 3 cut(s) 234, 266, 1422
BstMCI CGRYCG 1 cut(s) 265
BstNI CCWGG 1 cut(s) 231
BstNSI RCATGY 1 cut(s) 1154
BstSCI CCNGG 1 cut(s) 229
BstSLI GKGCMC 2 cut(s) 693, 829
BstUI CGCG 2 cut(s) 96, 171
BstV1I GCAGC 2 cut(s) 270, 1150
BstV2I GAAGAC 1 cut(s) 626
BstX2I RGATCY 2 cut(s) 234, 1422
BstYI RGATCY 2 cut(s) 234, 1422
BsuRI GGCC 1 cut(s) 204
BtgZI GCGATG 1 cut(s) 1059
BtrI CACGTC 1 cut(s) 324
BtsCI GGATG 6 cut(s) 162, 514, 868, 943, 1024, 1117
BtsIMutI CAGTG 1 cut(s) 126
Cac8I GCNNGC 4 cut(s) 227, 375, 532, 1083
CciI TCATGA 1 cut(s) 1267
CfoI GCGC 1 cut(s) 530
Cfr10I RCCGGY 2 cut(s) 530, 989
Cfr13I GGNCC 1 cut(s) 202
CseI GACGC 2 cut(s) 85, 284
Csp6I GTAC 1 cut(s) 605
CviAII CATG 5 cut(s) 151, 1039, 1151, 1247, 1268
CviJI RGCY 7 cut(s) 5, 204, 214, 225, 670, 806, 1163
CviKI_1 RGCY 7 cut(s) 5, 204, 214, 225, 670, 806, 1163
CviQI GTAC 1 cut(s) 605
DdeI CTNAG 4 cut(s) 221, 722, 875, 1379
DinI GGCGCC 1 cut(s) 529
DpnI GATC 3 cut(s) 236, 268, 1424
DpnII GATC 3 cut(s) 234, 266, 1422
DraIII CACNNNGTG 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 123, 539
EarI CTCTTC 2 cut(s) 123, 539
EciI GGCGGA 2 cut(s) 875, 1116
Eco31I GGTCTC 1 cut(s) 486
Eco88I CYCGRG 3 cut(s) 138, 341, 413
EcoRI GAATTC 1 cut(s) 1408
EcoRII CCWGG 1 cut(s) 229
EgeI GGCGCC 1 cut(s) 529
EheI GGCGCC 1 cut(s) 529
FaeI CATG 5 cut(s) 154, 1042, 1154, 1250, 1271
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FaqI GGGAC 1 cut(s) 143
FatI CATG 5 cut(s) 150, 1038, 1150, 1246, 1267
FauI CCCGC 1 cut(s) 248
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 3 cut(s) 147, 259, 1164
FokI GGATG 6 cut(s) 149, 521, 875, 950, 1031, 1124
Fsp4HI GCNGC 3 cut(s) 147, 259, 1164
FspBI CTAG 3 cut(s) 453, 885, 1347
GlaI GCGC 1 cut(s) 529
GluI GCNGC 3 cut(s) 147, 259, 1164
GsaI CCCAGC 1 cut(s) 1110
GsuI CTGGAG 2 cut(s) 29, 194
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 1 cut(s) 204
HapII CCGG 3 cut(s) 308, 531, 990
HgaI GACGC 2 cut(s) 85, 284
HhaI GCGC 1 cut(s) 530
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 5 cut(s) 154, 1042, 1154, 1250, 1271
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HinfI GANTC 9 cut(s) 110, 131, 141, 173, 197, 724, 754, 1028, 1094
HpaII CCGG 3 cut(s) 308, 531, 990
HphI GGTGA 7 cut(s) 43, 105, 126, 272, 279, 358, 923
Hpy166II GTNNAC 3 cut(s) 274, 691, 928
Hpy188I TCNGA 4 cut(s) 178, 618, 723, 899
Hpy188III TCNNGA 6 cut(s) 8, 308, 413, 556, 1268, 1395
Hpy8I GTNNAC 3 cut(s) 274, 691, 928
Hpy99I CGWCG 2 cut(s) 101, 328
HpyAV CCTTC 2 cut(s) 64, 227
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 3 cut(s) 323, 930, 1056
HpyCH4V TGCA 4 cut(s) 691, 941, 1004, 1337
HpyF3I CTNAG 4 cut(s) 221, 722, 875, 1379
HpySE526I ACGT 3 cut(s) 323, 930, 1056
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 5 cut(s) 154, 1042, 1154, 1250, 1271
HspAI GCGC 1 cut(s) 528
KasI GGCGCC 1 cut(s) 527
Kpn2I TCCGGA 1 cut(s) 307
KroI GCCGGC 1 cut(s) 530
KroNI GCCGGC 1 cut(s) 532
Kzo9I GATC 3 cut(s) 234, 266, 1422
LmnI GCTCC 4 cut(s) 219, 803, 1129, 1397
Lsp1109I GCAGC 2 cut(s) 270, 1150
LweI GCATC 4 cut(s) 928, 1093, 1200, 1346
MaeI CTAG 3 cut(s) 453, 885, 1347
MaeII ACGT 3 cut(s) 323, 930, 1056
MaeIII GTNAC 1 cut(s) 1034
MalI GATC 3 cut(s) 236, 268, 1424
MboI GATC 3 cut(s) 234, 266, 1422
MboII GAAGA 5 cut(s) 110, 449, 556, 631, 917
MflI RGATCY 2 cut(s) 234, 1422
MhlI GDGCHC 2 cut(s) 693, 829
MluCI AATT 8 cut(s) 356, 401, 586, 647, 1067, 1119, 1303, 1408
Mly113I GGCGCC 1 cut(s) 528
MlyI GAGTC 7 cut(s) 104, 125, 150, 182, 206, 733, 1037
MmeI TCCRAC 3 cut(s) 99, 201, 1270
MreI CGCCGGCG 1 cut(s) 530
MroI TCCGGA 1 cut(s) 307
MroNI GCCGGC 1 cut(s) 530
MroXI GAANNNNTTC 4 cut(s) 20, 753, 893, 1263
MseI TTAA 4 cut(s) 573, 821, 975, 1371
MslI CAYNNNNRTG 2 cut(s) 921, 1238
MspI CCGG 3 cut(s) 308, 531, 990
MspR9I CCNGG 1 cut(s) 231
Mva1269I GAATGC 2 cut(s) 408, 585
MvaI CCWGG 1 cut(s) 231
MvnI CGCG 2 cut(s) 96, 171
NaeI GCCGGC 1 cut(s) 532
NarI GGCGCC 1 cut(s) 528
NdeII GATC 3 cut(s) 234, 266, 1422
NgoMIV GCCGGC 1 cut(s) 530
NlaIII CATG 5 cut(s) 154, 1042, 1154, 1250, 1271
NlaIV GGNNCC 2 cut(s) 529, 705
NmuCI GTSAC 1 cut(s) 1034
NspI RCATGY 1 cut(s) 1154
NspV TTCGAA 3 cut(s) 24, 542, 757
PagI TCATGA 1 cut(s) 1267
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PctI GAATGC 2 cut(s) 408, 585
PdiI GCCGGC 1 cut(s) 532
PdmI GAANNNNTTC 4 cut(s) 20, 753, 893, 1263
PfeI GAWTC 2 cut(s) 754, 1094
PkrI GCNGC 3 cut(s) 148, 260, 1165
PleI GAGTC 7 cut(s) 104, 125, 149, 181, 205, 732, 1036
PluTI GGCGCC 1 cut(s) 531
PpsI GAGTC 7 cut(s) 104, 125, 149, 181, 205, 732, 1036
Psp6I CCWGG 1 cut(s) 229
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 2 cut(s) 529, 705
PspPI GGNCC 1 cut(s) 202
PsrI GAACNNNNNNTAC 2 cut(s) 597, 629
PsuI RGATCY 2 cut(s) 234, 1422
RsaI GTAC 1 cut(s) 606
RsaNI GTAC 1 cut(s) 605
RseI CAYNNNNRTG 2 cut(s) 921, 1238
SaqAI TTAA 4 cut(s) 573, 821, 975, 1371
SatI GCNGC 3 cut(s) 147, 259, 1164
Sau3AI GATC 3 cut(s) 234, 266, 1422
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 7 cut(s) 104, 125, 150, 182, 206, 733, 1037
ScrFI CCNGG 1 cut(s) 231
SduI GDGCHC 2 cut(s) 693, 829
SfaNI GCATC 4 cut(s) 928, 1093, 1200, 1346
SfoI GGCGCC 1 cut(s) 529
SfuI TTCGAA 3 cut(s) 24, 542, 757
SgrAI CRCCGGYG 1 cut(s) 530
SmiMI CAYNNNNRTG 2 cut(s) 921, 1238
SpeI ACTAGT 1 cut(s) 1346
Sse9I AATT 8 cut(s) 356, 401, 586, 647, 1067, 1119, 1303, 1408
SsiI CCGC 7 cut(s) 94, 147, 169, 241, 290, 860, 1127
SspDI GGCGCC 1 cut(s) 527
SspMI CTAG 3 cut(s) 453, 885, 1347
StyD4I CCNGG 1 cut(s) 229
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 3 cut(s) 326, 933, 1059
TaqI TCGA 6 cut(s) 24, 108, 542, 744, 757, 1134
TaqII GACCGA 2 cut(s) 279, 1212
TasI AATT 8 cut(s) 356, 401, 586, 647, 1067, 1119, 1303, 1408
TauI GCSGC 1 cut(s) 149
TfiI GAWTC 2 cut(s) 754, 1094
Tru1I TTAA 4 cut(s) 573, 821, 975, 1371
Tru9I TTAA 4 cut(s) 573, 821, 975, 1371
TscAI CASTG 1 cut(s) 133
TseFI GTSAC 1 cut(s) 1034
TseI GCWGC 2 cut(s) 258, 1163
Tsp45I GTSAC 1 cut(s) 1034
TspDTI ATGAA 6 cut(s) 37, 450, 993, 1049, 1256, 1293
TspGWI ACGGA 4 cut(s) 259, 614, 959, 997
TspRI CASTG 1 cut(s) 133
VneI GTGCAC 1 cut(s) 689
XapI RAATTY 5 cut(s) 401, 647, 1067, 1303, 1408
XceI RCATGY 1 cut(s) 1154
XmiI GTMKAC 1 cut(s) 273
XmnI GAANNNNTTC 4 cut(s) 20, 753, 893, 1263
XspI CTAG 3 cut(s) 453, 885, 1347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.