AT5G66850

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001271
Physical Location & Seq
Reverse (-)
26695568 .. 26699426
3859 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G66850.1

Sequence Viewer

Length: 2151 bp
ATGCGTTGGCTTCCGCAAATCTCGTTCTCGTCTCCTTCTTCTTCTCCATCTTCTTCTCTAAAACCCGTGGCTTCTTACTCTGAATCTCCGGATCCAGATCGTAATCAGGATCGGGATCGGTTTCATCGCCGCTTGTTTCGTTTTAACCGTGGCAGGCTCACCCGTCAGCGGAAGCTTCGTCACTTGACGGATGACGATGTTTTGTTGGGAGAACGTCGTGCTTCTACCTCTTCCTCCACCTTCGATTCCGGTTTAACTCGCTCTCCCAGCGCTTTCACCGCCGTTCCTCGCTCTCCTTCTGCCGTTCCCTTACCCTTACCTCTCCCCTTACCGGAGGTCGCCGGGATTCGAAATGCCGCAAACGCTAGAGGATTGGATGACAGAGATCGAGATCCCGAGAGACTTATTTCTGATCGCACCTCTTCTGGTCCTCCTCTCACCAGCGTCAATGGCGGCTTTGCCCGTGACTCGAGGAAAGCTACGGAGAATTCGTCATATCAAGATTTTAGTCCGAGAAACAGAAATGGTTATTGGGTGAATATTCCAACCATGAGTGCACCAACGAGTCCATACATGAGTCCTGTGCCTAGTCCACAAAGGAAGAGTACTGGCCACGATTTGCCTTTTTTTTATTTGCCTCCTAAAAGCAATCAAGCTTGGTCTGCTCCAGATATGCCACTTGATACCTCTGGTCTTCCTCCTCCTGCATTTTATGATATTACTGCCTTTAGTACCGATAATTCTCCCATCCATAGTCCACAACCTCGGAGTCCACGGAAACAGATCAGAAGCCCACAACCTAGCAGACCATCTTCACCGTTGCATTCGGTTGATAGCTCAGCTCCACCGCGAGATAGTGTTTCCTCACCTTTGCATCCGAGGTTGTCTACTGATGTTACGAATGGGCGACGTGATTGCTGCAATGTTCATCCTTTGCCTCTCCCTCCTGGAGCTACTTGTTCCTCTTCATCAGCTGCTTCTGTTCCGTCCCCGCAGGCTCCTCTCAAACTGGATTCATTCCCAATGAATTCCCAGTGGAAGAAAGGGAAGCTAATAGGTCGTGGTACTTTTGGAAGTGTTTACGTTGCAAGCAACAGCGAAACTGGAGCATTGTGTGCGATGAAAGAAGTTGAGCTATTTCCTGATGACCCCAAATCCGCAGAGTGTATAAAGCAATTAGAGCAGGAGATCAAACTTCTAAGTAACCTTCAACATCCAAACATTGTGCAGTATTTTGGTAGCGAGACAGTAGAAGATCGTTTCTTTATATACCTGGAATATGTTCACCCGGGTTCAATAAACAAATATATCCGTGATCATTGCGGCACCATGACAGAATCTGTTGTTCGCAATTTTACTCGTCACATTTTGTCTGGGCTGGCTTATTTGCACAATAAAAAGACTGTACATAGGGATATCAAAGGTGCTAATCTCCTTGTTGATGCTTCTGGGGTTGTCAAGCTTGCTGATTTCGGCATGGCTAAACACCTTACTGGACAAAGAGCTGATCTCTCGTTAAAGGGAAGCCCGTACTGGATGGCACCAGAGCTCATGCAAGCTGTGATGCAAAAAGATAGCAACCCAGATCTGGCTTTTGCTGTTGATATATGGAGTTTAGGATGTACAATCATTGAGATGTTCACTGGGAAGCCTCCTTGGAGTGAGTTTGAAGGGGCTGCAGCTATGTTCAAGGTCATGAGAGATAGCCCACCGATACCTGAATCAATGTCACCTGAGGGTAAAGACTTCCTGAGATTATGCTTCCAGAGAAACCCAGCTGAGCGACCAACCGCATCTATGTTGCTAGAACACCGGTTCCTAAAGAACTCTTTGCAACCAACCTCACCAAGCAACAGTGATGTCTCTCAATTATTTAATGGGATGAACATAACGGAACCAAGCAGTAGAAGGGAGAAGCCAAATTTCAAACTAGACCAGGTCCCGCGAGCTAGAAACATGACATCCTCAGAGAGTGAAAGTGGGCAACAGCAGCAGCAACAACAGTACCGGTCTCCCGATCTAACAGGAACCGTGAACCGTCTGTCTCCTCGTTCCACTCTGGAGGCTATCCCAAGCCCGTGTCCTTCCCAACGACCTAAGCCCAGCAGCAGTGACAGGAGAAGAACGGGCGTCACTTCAGATCACCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

716

Amino Acids

79.32

Weight (kDa)

9.39

Isoelectric Point (pI)

68.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 347 - 607 1.4e-66 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 349 - 603 3.1e-45 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 460 - 557 2.4e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1325, 1540
AccI GTMKAC 1 cut(s) 887
AccII CGCG 2 cut(s) 850, 1945
AccIII TCCGGA 1 cut(s) 88
AclWI GGATC 5 cut(s) 86, 99, 117, 123, 386
AcoI YGGCCR 1 cut(s) 610
AcsI RAATTY 3 cut(s) 487, 1027, 1921
AcuI CTGAAG 1 cut(s) 2121
AcyI GRCGYC 1 cut(s) 2130
AfaI GTAC 7 cut(s) 607, 733, 1066, 1407, 1532, 1624, 2006
AfeI AGCGCT 1 cut(s) 271
AfiI CCNNNNNNNGG 3 cut(s) 168, 331, 1588
AgeI ACCGGT 2 cut(s) 1812, 2007
AgsI TTSAA 5 cut(s) 1211, 1296, 1670, 1690, 1927
AjiI CACGTC 1 cut(s) 911
AjnI CCWGG 3 cut(s) 946, 1272, 1935
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
AloI GAACNNNNNNTCC 2 cut(s) 1800, 1832
Alw21I GWGCWC 2 cut(s) 559, 1551
Alw26I GTCTC 6 cut(s) 36, 394, 1238, 1867, 2016, 2049
Alw44I GTGCAC 1 cut(s) 555
AlwI GGATC 5 cut(s) 86, 99, 117, 123, 386
AlwNI CAGNNNCTG 1 cut(s) 1340
Ama87I CYCGRG 3 cut(s) 395, 469, 1288
Aor13HI TCCGGA 1 cut(s) 88
Aor51HI AGCGCT 1 cut(s) 271
AoxI GGCC 1 cut(s) 610
ApaLI GTGCAC 1 cut(s) 555
ApeKI GCWGC 7 cut(s) 918, 974, 1676, 1679, 1990, 1993, 2106
ApoI RAATTY 3 cut(s) 487, 1027, 1921
AsiGI ACCGGT 2 cut(s) 1812, 2007
Asp700I GAANNNNTTC 1 cut(s) 1281
AspLEI GCGC 1 cut(s) 272
AspS9I GGNCC 2 cut(s) 428, 1939
AsuC2I CCSGG 3 cut(s) 343, 1289, 1290
AsuII TTCGAA 1 cut(s) 349
AvaI CYCGRG 3 cut(s) 395, 469, 1288
AvaII GGWCC 2 cut(s) 428, 1939
AxyI CCTNAGG 1 cut(s) 1734
BaeGI GKGCMC 1 cut(s) 559
BaeI ACNNNNGTAYC 2 cut(s) 1988, 2021
BalI TGGCCA 1 cut(s) 612
BamHI GGATCC 1 cut(s) 91
BanI GGYRCC 2 cut(s) 1325, 1540
BanII GRGCYC 1 cut(s) 1551
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 2 cut(s) 559, 1551
BbvI GCAGC 7 cut(s) 905, 961, 1663, 1691, 2002, 2005, 2118
BccI CCATC 4 cut(s) 55, 755, 817, 1531
BceAI ACGGC 2 cut(s) 266, 287
BcgI CGANNNNNNTGC 2 cut(s) 897, 931
BciT130I CCWGG 3 cut(s) 948, 1274, 1937
BclI TGATCA 1 cut(s) 1315
BcnI CCSGG 3 cut(s) 343, 1289, 1290
BcoDI GTCTC 6 cut(s) 36, 394, 1238, 1867, 2016, 2049
BfaI CTAG 6 cut(s) 366, 588, 801, 1805, 1931, 1950
BfmI CTRYAG 1 cut(s) 1677
BfoI RGCGCY 1 cut(s) 273
BglII AGATCT 1 cut(s) 1585
BlpI GCTNAGC 2 cut(s) 838, 1779
BmcAI AGTACT 1 cut(s) 607
Bme1390I CCNGG 6 cut(s) 343, 948, 1274, 1289, 1290, 1937
Bme18I GGWCC 2 cut(s) 428, 1939
BmeT110I CYCGRG 3 cut(s) 395, 469, 1288
BmgBI CACGTC 1 cut(s) 911
BmgT120I GGNCC 2 cut(s) 428, 1939
BmiI GGNNCC 8 cut(s) 93, 999, 1327, 1542, 1817, 1896, 1941, 2029
BmrFI CCNGG 6 cut(s) 343, 948, 1274, 1289, 1290, 1937
BmrI ACTGGG 2 cut(s) 1027, 1653
BmsI GCATC 4 cut(s) 883, 1432, 1554, 1802
BmuI ACTGGG 2 cut(s) 1027, 1653
BpiI GAAGAC 1 cut(s) 686
BplI GAGNNNNNCTC 2 cut(s) 1494, 1526
BpmI CTGGAG 4 cut(s) 651, 969, 1125, 2081
Bpu10I CCTNAGC 1 cut(s) 2097
Bpu1102I GCTNAGC 2 cut(s) 838, 1779
Bpu14I TTCGAA 1 cut(s) 349
BpuMI CCSGG 3 cut(s) 343, 1289, 1290
BsaBI GATNNNNATC 4 cut(s) 96, 102, 114, 390
BsaHI GRCGYC 1 cut(s) 2130
BsaI GGTCTC 1 cut(s) 2016
BsaJI CCNNGG 7 cut(s) 66, 148, 764, 773, 878, 1288, 1655
BsaWI WCCGGW 5 cut(s) 88, 248, 331, 1812, 2007
BsaXI ACNNNNNCTCC 4 cut(s) 247, 277, 476, 506
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 331, 1588
Bse118I RCCGGY 2 cut(s) 1812, 2007
Bse1I ACTGG 7 cut(s) 613, 1014, 1033, 1108, 1498, 1538, 1648
Bse21I CCTNAGG 1 cut(s) 1734
Bse3DI GCAATG 2 cut(s) 928, 1318
Bse8I GATNNNNATC 4 cut(s) 96, 102, 114, 390
BseAI TCCGGA 1 cut(s) 88
BseBI CCWGG 3 cut(s) 948, 1274, 1937
BseDI CCNNGG 7 cut(s) 66, 148, 764, 773, 878, 1288, 1655
BseJI GATNNNNATC 4 cut(s) 96, 102, 114, 390
BseLI CCNNNNNNNGG 3 cut(s) 168, 331, 1588
BseMI GCAATG 2 cut(s) 928, 1318
BseMII CTCAG 5 cut(s) 852, 1725, 1742, 1770, 1980
BseNI ACTGG 7 cut(s) 613, 1014, 1033, 1108, 1498, 1538, 1648
BseRI GAGGAG 4 cut(s) 423, 690, 990, 2037
BseSI GKGCMC 1 cut(s) 559
BseXI GCAGC 7 cut(s) 905, 961, 1663, 1691, 2002, 2005, 2118
BseYI CCCAGC 3 cut(s) 266, 1774, 2102
BsgI GTGCAG 1 cut(s) 1247
Bsh1236I CGCG 2 cut(s) 850, 1945
BshFI GGCC 1 cut(s) 612
BshNI GGYRCC 2 cut(s) 1325, 1540
BshTI ACCGGT 2 cut(s) 1812, 2007
BsiHKAI GWGCWC 2 cut(s) 559, 1551
BsiHKCI CYCGRG 3 cut(s) 395, 469, 1288
BsiSI CCGG 7 cut(s) 89, 249, 332, 342, 1289, 1813, 2008
BslFI GGGAC 2 cut(s) 973, 1925
BslI CCNNNNNNNGG 3 cut(s) 168, 331, 1588
BsmAI GTCTC 6 cut(s) 36, 394, 1238, 1867, 2016, 2049
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 2 cut(s) 973, 1925
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 1 cut(s) 612
Bso31I GGTCTC 1 cut(s) 2016
BsoBI CYCGRG 3 cut(s) 395, 469, 1288
Bsp119I TTCGAA 1 cut(s) 349
Bsp1286I GDGCHC 2 cut(s) 559, 1551
Bsp13I TCCGGA 1 cut(s) 88
Bsp1407I TGTACA 2 cut(s) 1405, 1622
Bsp1720I GCTNAGC 2 cut(s) 838, 1779
BspANI GGCC 1 cut(s) 612
BspCNI CTCAG 5 cut(s) 851, 1726, 1743, 1771, 1979
BspEI TCCGGA 1 cut(s) 88
BspFNI CGCG 2 cut(s) 850, 1945
BspHI TCATGA 1 cut(s) 1695
BspLI GGNNCC 8 cut(s) 93, 999, 1327, 1542, 1817, 1896, 1941, 2029
BspMAI CTGCAG 1 cut(s) 1681
BspPI GGATC 5 cut(s) 86, 99, 117, 123, 386
BspT104I TTCGAA 1 cut(s) 349
BspT107I GGYRCC 2 cut(s) 1325, 1540
BspTNI GGTCTC 1 cut(s) 2016
BsrDI GCAATG 2 cut(s) 928, 1318
BsrFI RCCGGY 2 cut(s) 1812, 2007
BsrGI TGTACA 2 cut(s) 1405, 1622
BsrI ACTGG 7 cut(s) 613, 1014, 1033, 1108, 1498, 1538, 1648
BssAI RCCGGY 2 cut(s) 1812, 2007
BssECI CCNNGG 7 cut(s) 66, 148, 764, 773, 878, 1288, 1655
BssNI GRCGYC 1 cut(s) 2130
BssT1I CCWWGG 1 cut(s) 1655
Bst2UI CCWGG 3 cut(s) 948, 1274, 1937
Bst4CI ACNGT 8 cut(s) 149, 819, 1249, 1405, 1856, 2004, 2032, 2039
Bst6I CTCTTC 4 cut(s) 235, 427, 596, 970
BstACI GRCGYC 1 cut(s) 2130
BstAPI GCANNNNNTGC 1 cut(s) 1115
BstAUI TGTACA 2 cut(s) 1405, 1622
BstBI TTCGAA 1 cut(s) 349
BstC8I GCNNGC 7 cut(s) 155, 996, 1090, 1380, 1464, 1557, 1947
BstDEI CTNAG 7 cut(s) 838, 1199, 1734, 1751, 1779, 1966, 2097
BstDSI CCRYGG 3 cut(s) 66, 148, 773
BstFNI CGCG 2 cut(s) 850, 1945
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstMAI GTCTC 6 cut(s) 36, 394, 1238, 1867, 2016, 2049
BstMWI GCNNNNNNNGC 8 cut(s) 267, 278, 362, 450, 662, 1115, 1180, 1990
BstNI CCWGG 3 cut(s) 948, 1274, 1937
BstSCI CCNGG 6 cut(s) 341, 946, 1272, 1287, 1288, 1935
BstSFI CTRYAG 1 cut(s) 1677
BstSLI GKGCMC 1 cut(s) 559
BstUI CGCG 2 cut(s) 850, 1945
BstV1I GCAGC 7 cut(s) 905, 961, 1663, 1691, 2002, 2005, 2118
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 3 cut(s) 91, 391, 1585
BstYI RGATCY 3 cut(s) 91, 391, 1585
Bsu36I CCTNAGG 1 cut(s) 1734
BsuRI GGCC 1 cut(s) 612
BtgI CCRYGG 3 cut(s) 66, 148, 773
BtgZI GCGATG 2 cut(s) 110, 1133
BtrI CACGTC 1 cut(s) 911
BtsI GCAGTG 1 cut(s) 2116
BtsIMutI CAGTG 4 cut(s) 1040, 1641, 1861, 2116
Cac8I GCNNGC 7 cut(s) 155, 996, 1090, 1380, 1464, 1557, 1947
CaiI CAGNNNCTG 1 cut(s) 1340
CciI TCATGA 1 cut(s) 1695
CfoI GCGC 1 cut(s) 272
Cfr10I RCCGGY 2 cut(s) 1812, 2007
Cfr13I GGNCC 2 cut(s) 428, 1939
Cfr9I CCCGGG 1 cut(s) 1288
CseI GACGC 2 cut(s) 433, 2119
CsiI ACCWGGT 1 cut(s) 1935
Csp6I GTAC 7 cut(s) 606, 732, 1065, 1406, 1531, 1623, 2005
CspAI ACCGGT 2 cut(s) 1812, 2007
CviAII CATG 7 cut(s) 550, 574, 1330, 1477, 1552, 1696, 1957
CviQI GTAC 7 cut(s) 606, 732, 1065, 1406, 1531, 1623, 2005
DdeI CTNAG 7 cut(s) 838, 1199, 1734, 1751, 1779, 1966, 2097
EaeI YGGCCR 1 cut(s) 610
Eam1104I CTCTTC 4 cut(s) 235, 427, 596, 970
EarI CTCTTC 4 cut(s) 235, 427, 596, 970
Ecl136II GAGCTC 1 cut(s) 1549
Eco130I CCWWGG 1 cut(s) 1655
Eco24I GRGCYC 1 cut(s) 1551
Eco31I GGTCTC 1 cut(s) 2016
Eco32I GATATC 1 cut(s) 1417
Eco47I GGWCC 2 cut(s) 428, 1939
Eco47III AGCGCT 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 1549
Eco57I CTGAAG 1 cut(s) 2121
Eco81I CCTNAGG 1 cut(s) 1734
Eco88I CYCGRG 3 cut(s) 395, 469, 1288
EcoICRI GAGCTC 1 cut(s) 1549
EcoO109I RGGNCCY 1 cut(s) 1939
EcoRI GAATTC 2 cut(s) 487, 1027
EcoRII CCWGG 3 cut(s) 946, 1272, 1935
EcoRV GATATC 1 cut(s) 1417
EcoT14I CCWWGG 1 cut(s) 1655
EcoT38I GRGCYC 1 cut(s) 1551
ErhI CCWWGG 1 cut(s) 1655
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 7 cut(s) 553, 577, 1333, 1480, 1555, 1699, 1960
FaqI GGGAC 2 cut(s) 973, 1925
FatI CATG 7 cut(s) 549, 573, 1329, 1476, 1551, 1695, 1956
FauI CCCGC 2 cut(s) 999, 1950
FbaI TGATCA 1 cut(s) 1315
FblI GTMKAC 1 cut(s) 887
FriOI GRGCYC 1 cut(s) 1551
FspBI CTAG 6 cut(s) 366, 588, 801, 1805, 1931, 1950
GlaI GCGC 1 cut(s) 271
GsaI CCCAGC 3 cut(s) 270, 1778, 2106
GsuI CTGGAG 4 cut(s) 651, 969, 1125, 2081
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 1 cut(s) 612
HapII CCGG 7 cut(s) 89, 249, 332, 342, 1289, 1813, 2008
HgaI GACGC 2 cut(s) 433, 2119
HhaI GCGC 1 cut(s) 272
Hin1I GRCGYC 1 cut(s) 2130
Hin1II CATG 7 cut(s) 553, 577, 1333, 1480, 1555, 1699, 1960
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HindIII AAGCTT 3 cut(s) 173, 654, 1460
HpaII CCGG 7 cut(s) 89, 249, 332, 342, 1289, 1813, 2008
Hpy166II GTNNAC 9 cut(s) 557, 593, 758, 773, 888, 1081, 1285, 1641, 2035
Hpy188I TCNGA 8 cut(s) 82, 412, 513, 768, 788, 879, 1969, 2140
Hpy8I GTNNAC 9 cut(s) 557, 593, 758, 773, 888, 1081, 1285, 1641, 2035
Hpy99I CGWCG 2 cut(s) 219, 912
HpyAV CCTTC 7 cut(s) 45, 250, 306, 1217, 1664, 1902, 2094
HpyCH4III ACNGT 8 cut(s) 149, 819, 1249, 1405, 1856, 2004, 2032, 2039
HpyCH4IV ACGT 3 cut(s) 214, 910, 1083
HpyF10VI GCNNNNNNNGC 8 cut(s) 267, 278, 362, 450, 662, 1115, 1180, 1990
HpyF3I CTNAG 7 cut(s) 838, 1199, 1734, 1751, 1779, 1966, 2097
HpySE526I ACGT 3 cut(s) 214, 910, 1083
Hsp92I GRCGYC 1 cut(s) 2130
Hsp92II CATG 7 cut(s) 553, 577, 1333, 1480, 1555, 1699, 1960
HspAI GCGC 1 cut(s) 270
Kpn2I TCCGGA 1 cut(s) 88
Ksp22I TGATCA 1 cut(s) 1315
LmnI GCTCC 5 cut(s) 670, 847, 950, 1003, 1106
Lsp1109I GCAGC 7 cut(s) 905, 961, 1663, 1691, 2002, 2005, 2118
LweI GCATC 4 cut(s) 883, 1432, 1554, 1802
MabI ACCWGGT 1 cut(s) 1935
MaeI CTAG 6 cut(s) 366, 588, 801, 1805, 1931, 1950
MaeII ACGT 3 cut(s) 214, 910, 1083
MaeIII GTNAC 8 cut(s) 179, 464, 895, 1202, 1361, 1728, 2111, 2131
MflI RGATCY 3 cut(s) 91, 391, 1585
MhlI GDGCHC 2 cut(s) 559, 1551
MlsI TGGCCA 1 cut(s) 612
MluCI AATT 7 cut(s) 487, 739, 1027, 1175, 1351, 1868, 1921
MluNI TGGCCA 1 cut(s) 612
MlyI GAGTC 4 cut(s) 461, 574, 586, 778
MmeI TCCRAC 1 cut(s) 569
Mox20I TGGCCA 1 cut(s) 612
MroI TCCGGA 1 cut(s) 88
MroXI GAANNNNTTC 1 cut(s) 1281
MscI TGGCCA 1 cut(s) 612
MseI TTAA 4 cut(s) 144, 254, 1517, 1875
MslI CAYNNNNRTG 1 cut(s) 1634
Msp20I TGGCCA 1 cut(s) 612
MspA1I CMGCKG 3 cut(s) 169, 974, 1778
MspI CCGG 7 cut(s) 89, 249, 332, 342, 1289, 1813, 2008
MspR9I CCNGG 6 cut(s) 343, 948, 1274, 1289, 1290, 1937
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 3 cut(s) 948, 1274, 1937
MvnI CGCG 2 cut(s) 850, 1945
MwoI GCNNNNNNNGC 8 cut(s) 267, 278, 362, 450, 662, 1115, 1180, 1990
NciI CCSGG 3 cut(s) 343, 1289, 1290
NlaIII CATG 7 cut(s) 553, 577, 1333, 1480, 1555, 1699, 1960
NlaIV GGNNCC 8 cut(s) 93, 999, 1327, 1542, 1817, 1896, 1941, 2029
NmuCI GTSAC 6 cut(s) 179, 464, 1361, 1728, 2111, 2131
NspV TTCGAA 1 cut(s) 349
PaeR7I CTCGAG 1 cut(s) 469
PagI TCATGA 1 cut(s) 1695
PcsI WCGNNNNNNNCGW 2 cut(s) 145, 488
PctI GAATGC 1 cut(s) 823
PdmI GAANNNNTTC 1 cut(s) 1281
PfeI GAWTC 6 cut(s) 83, 245, 346, 1013, 1337, 1721
PflFI GACNNNGTC 1 cut(s) 1937
PfoI TCCNGGA 1 cut(s) 946
PinAI ACCGGT 2 cut(s) 1812, 2007
PleI GAGTC 4 cut(s) 461, 573, 585, 777
PpsI GAGTC 4 cut(s) 461, 573, 585, 777
PpuMI RGGWCCY 1 cut(s) 1939
Psp124BI GAGCTC 1 cut(s) 1551
Psp5II RGGWCCY 1 cut(s) 1939
Psp6I CCWGG 3 cut(s) 946, 1272, 1935
PspFI CCCAGC 3 cut(s) 266, 1774, 2102
PspGI CCWGG 3 cut(s) 946, 1272, 1935
PspN4I GGNNCC 8 cut(s) 93, 999, 1327, 1542, 1817, 1896, 1941, 2029
PspPI GGNCC 2 cut(s) 428, 1939
PspPPI RGGWCCY 1 cut(s) 1939
PspXI VCTCGAGB 1 cut(s) 469
PstI CTGCAG 1 cut(s) 1681
PstNI CAGNNNCTG 1 cut(s) 1340
PsuI RGATCY 3 cut(s) 91, 391, 1585
PsyI GACNNNGTC 1 cut(s) 1937
PvuII CAGCTG 2 cut(s) 974, 1778
RsaI GTAC 7 cut(s) 607, 733, 1066, 1407, 1532, 1624, 2006
RsaNI GTAC 7 cut(s) 606, 732, 1065, 1406, 1531, 1623, 2005
RseI CAYNNNNRTG 1 cut(s) 1634
SacI GAGCTC 1 cut(s) 1551
SaqAI TTAA 4 cut(s) 144, 254, 1517, 1875
Sau96I GGNCC 2 cut(s) 428, 1939
ScaI AGTACT 1 cut(s) 607
SchI GAGTC 4 cut(s) 461, 574, 586, 778
ScrFI CCNGG 6 cut(s) 343, 948, 1274, 1289, 1290, 1937
SduI GDGCHC 2 cut(s) 559, 1551
SexAI ACCWGGT 1 cut(s) 1935
SfaNI GCATC 4 cut(s) 883, 1432, 1554, 1802
SfcI CTRYAG 1 cut(s) 1677
Sfr274I CTCGAG 1 cut(s) 469
SfuI TTCGAA 1 cut(s) 349
SinI GGWCC 2 cut(s) 428, 1939
SlaI CTCGAG 1 cut(s) 469
SmaI CCCGGG 1 cut(s) 1290
SmiMI CAYNNNNRTG 1 cut(s) 1634
SmlI CTYRAG 1 cut(s) 469
SmoI CTYRAG 1 cut(s) 469
Sse9I AATT 7 cut(s) 487, 739, 1027, 1175, 1351, 1868, 1921
SspI AATATT 1 cut(s) 541
SspMI CTAG 6 cut(s) 366, 588, 801, 1805, 1931, 1950
SstI GAGCTC 1 cut(s) 1551
StyD4I CCNGG 6 cut(s) 341, 946, 1272, 1287, 1288, 1935
StyI CCWWGG 1 cut(s) 1655
TaaI ACNGT 8 cut(s) 149, 819, 1249, 1405, 1856, 2004, 2032, 2039
TaiI ACGT 3 cut(s) 217, 913, 1086
TaqI TCGA 4 cut(s) 243, 349, 388, 470
TasI AATT 7 cut(s) 487, 739, 1027, 1175, 1351, 1868, 1921
TatI WGTACW 3 cut(s) 605, 1405, 1622
TauI GCSGC 4 cut(s) 132, 359, 456, 1326
TfiI GAWTC 6 cut(s) 83, 245, 346, 1013, 1337, 1721
Tru1I TTAA 4 cut(s) 144, 254, 1517, 1875
Tru9I TTAA 4 cut(s) 144, 254, 1517, 1875
TscAI CASTG 4 cut(s) 1040, 1648, 1861, 2116
TseFI GTSAC 6 cut(s) 179, 464, 1361, 1728, 2111, 2131
TseI GCWGC 7 cut(s) 918, 974, 1676, 1679, 1990, 1993, 2106
Tsp45I GTSAC 6 cut(s) 179, 464, 1361, 1728, 2111, 2131
TspDTI ATGAA 7 cut(s) 113, 917, 957, 1005, 1040, 1136, 1898
TspGWI ACGGA 6 cut(s) 203, 497, 790, 975, 1301, 1907
TspMI CCCGGG 1 cut(s) 1288
TspRI CASTG 4 cut(s) 1040, 1648, 1861, 2116
Tth111I GACNNNGTC 1 cut(s) 1937
VneI GTGCAC 1 cut(s) 555
VpaK11BI GGWCC 2 cut(s) 428, 1939
XapI RAATTY 3 cut(s) 487, 1027, 1921
XhoI CTCGAG 1 cut(s) 469
XmaI CCCGGG 1 cut(s) 1288
XmiI GTMKAC 1 cut(s) 887
XmnI GAANNNNTTC 1 cut(s) 1281
XspI CTAG 6 cut(s) 366, 588, 801, 1805, 1931, 1950
ZrmI AGTACT 1 cut(s) 607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.