FvH4_1g15530

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001271
Physical Location & Seq
Forward (+)
8820898 .. 8825169
4272 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g15530.t1

Sequence Viewer

Length: 1878 bp
ATGGACAATGGATCTTCTTCTCCAGGACCTCATCATCGTCGAGTCTTACGTGATTACACTTTTGTCCTCCTCCTCTTCCTCATGCTAGACTCCACCATGCGTTGGTTTGCTTCGTCGTCATCATCGTCTGCTCCCTCGTCGTCGTCATCGCCGAAGAAGAAGGGGAGGGAGGAGGGCGGGTGGCTTTTCCGGTCGAGGAGGAAGATTGCGCAGCAGCAGAGGAAGCTTCTTAACGTGGGGAGAGACTTTGACGGTGCAGTAAGCAAATGCCCCAGCGCGCCGGGGTCAGAGGCCGCGCTGGGGAGTCCGCAGCCTCTTCCTTTGCCGGAAGAGGCCAACCGCCGAGGGAGGAGGTGTTCTCGCTGCAGCTCATGGGGACATGACAACTGCATGCCCTTCCCGGCTCCCAGTGTGTTTTCTTGCCAAGATACTCCAAAGAATAACAATCAAGTACGTGAGACAGTATCGTCTAGGAGGAGTAACCGAGATCGAAATCGTTTACAGATCAATGAACATGATCTCAGGCTCGATTTTCCTGTTAGAAGTGCACCTACTAGTCCGTGCGCAAGTCCTAGATTCAGCCCACGCAGAACTCCTGGTGATTTGGGGACTCCTTCGAGAAATCAAGGCTGGTCTGCACCGGAGATGAATAGTAATGATATGACTCAACCTCCCTCTGCCTTCTTAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTCCCACCGGTAGAAGTCCCCTTCGACAACCACAAGGATCATCACCACTGCATCCCCTGTTATCTGTAACCTCTGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGTCTTCCTTCACCTAGTGTACCACCATCACCAAGCCTACTATCACCACAACCTGCAGTCACACCTAAGCAAGAGTCCCAGCCGATTAAAGGGCAATGGCAAAAGGGAAAGCTCATTGGTCGTGGCACGTTTGGCAGTGTTTATGTTGCTACCAATAGAAAAACTGGAGCTTTATGTGCAATGAAGGAAGTTGAACTACTTCCTGATGACCCGAAATCAGCAGAGTGTATAAAGCAGCTACAGCAGGAAATCAAAGTCCTCAGCCAGCTCAAGCATCCTAATATTGTTCAGTACTATGGAAGTGAAATTGTTGAAGATCGTTTTTTTATATACCTGGAGTATGTTCATCCTGGTTCTATTAACAAATATGTTCATGAACATTATGATGGAGCTATGACAGAAGCTGTTGTTCGCAGTTTTACTCGCCATATACTCTCTGGGTTGGCTTACTTGCACAGCAAAAAAACAATACACAGGGACATCAAAGGGGCTAATTTGCTTGTGGATTCATTTGGGGTTGTCAAGCTAGCTGACTTTGGAATGGCAAAACATCTTTCAGGACATGTAGGGAATCTTTCTTTAAAGGGAAGTCCATACTGGATGGCTCCAGAGCTGATGCAATCTGAGATGAATAAAGATAACAGCTCTGATTTGGCTCTTGCTGTTGATATATGGAGTTTGGGTTGTACTATTATTGAAATGTTCACAGGAAAACCTCCGTGGAGTCAGTATGAAGGGGCTGCAGCCATGTTTAAAGTCATTAAGGATACTCCACCAATTCCAGAAACATTGTCGCCTGAGGGTAAGGATTTCCTCAGGTGCTGCTTCCAAAGAAATCCTGCTGAGAGATCAACTGCTGCAATGCTATTAGACCATCGATTTGTAAAAAGCTCCCAGCCGCAGGAAGTCCCATCTACAACCTATGCCTCTAATGGGATGAATCTCATGGAAAGACCCCACACTCCAACTGAACTTGAAAGAAGACTTGATAGTTCAGTGATGATTCCCAACATCGGCATGCAATTGACTACGGGAAAGGCAGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

626

Amino Acids

68.99

Weight (kDa)

9.27

Isoelectric Point (pI)

67.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 309 - 571 4e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 312 - 565 7.1e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 466
Acc16I TGCGCA 2 cut(s) 210, 565
Acc36I ACCTGC 1 cut(s) 889
AccB7I CCANNNNNTGG 1 cut(s) 102
AccII CGCG 2 cut(s) 278, 296
AciI CCGC 5 cut(s) 177, 294, 308, 340, 1727
AclWI GGATC 2 cut(s) 19, 766
AcuI CTGAAG 1 cut(s) 816
AdeI CACNNNGTG 1 cut(s) 845
AfaI GTAC 4 cut(s) 453, 849, 1121, 1516
AfiI CCNNNNNNNGG 6 cut(s) 102, 300, 917, 1729, 1761, 1841
AflIII ACRYGT 1 cut(s) 1390
AgeI ACCGGT 1 cut(s) 728
AgsI TTSAA 4 cut(s) 1022, 1142, 1526, 1805
AhlI ACTAGT 1 cut(s) 554
AjnI CCWGG 5 cut(s) 22, 595, 820, 1161, 1177
AloI GAACNNNNNNTCC 2 cut(s) 340, 372
Alw21I GWGCWC 1 cut(s) 550
Alw26I GTCTC 2 cut(s) 237, 452
Alw44I GTGCAC 1 cut(s) 546
AlwI GGATC 2 cut(s) 19, 766
AlwNI CAGNNNCTG 2 cut(s) 1232, 1650
AoxI GGCC 2 cut(s) 291, 333
ApaLI GTGCAC 1 cut(s) 546
ArsI GACNNNNNNTTYG 2 cut(s) 1691, 1723
AsiGI ACCGGT 1 cut(s) 728
Asp700I GAANNNNTTC 1 cut(s) 1026
AspLEI GCGC 5 cut(s) 211, 278, 280, 298, 566
AspS9I GGNCC 1 cut(s) 26
AsuC2I CCSGG 2 cut(s) 282, 401
AsuHPI GGTGA 5 cut(s) 611, 756, 831, 849, 864
AsuNHI GCTAGC 1 cut(s) 1354
AvaII GGWCC 1 cut(s) 26
AxyI CCTNAGG 2 cut(s) 1626, 1643
BaeGI GKGCMC 1 cut(s) 550
BarI GAAGNNNNNNTAC 4 cut(s) 535, 567, 1008, 1040
BbsI GAAGAC 2 cut(s) 824, 1816
Bbv12I GWGCWC 1 cut(s) 550
BbvCI CCTCAGC 1 cut(s) 1088
BccI CCATC 6 cut(s) 810, 862, 1208, 1423, 1710, 1747
BciT130I CCWGG 5 cut(s) 24, 597, 822, 1163, 1179
BciVI GTATCC 1 cut(s) 1588
BcnI CCSGG 2 cut(s) 282, 401
BcoDI GTCTC 2 cut(s) 237, 452
BcuI ACTAGT 1 cut(s) 554
BfaI CTAG 6 cut(s) 86, 471, 555, 573, 843, 1355
BfmI CTRYAG 4 cut(s) 364, 882, 1067, 1569
BfuAI ACCTGC 1 cut(s) 889
BfuI GTATCC 1 cut(s) 1588
BmcAI AGTACT 1 cut(s) 1121
Bme1390I CCNGG 7 cut(s) 24, 282, 401, 597, 822, 1163, 1179
Bme18I GGWCC 1 cut(s) 26
BmgT120I GGNCC 1 cut(s) 26
BmiI GGNNCC 2 cut(s) 405, 1434
BmrFI CCNGG 7 cut(s) 24, 282, 401, 597, 822, 1163, 1179
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 3 cut(s) 781, 1111, 1434
BmtI GCTAGC 1 cut(s) 1358
BmuI ACTGGG 1 cut(s) 402
BpiI GAAGAC 2 cut(s) 824, 1816
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpmI CTGGAG 5 cut(s) 6, 843, 1014, 1184, 1419
Bpu10I CCTNAGC 2 cut(s) 894, 1088
BpuEI CTTGAG 1 cut(s) 1082
BpuMI CCSGG 2 cut(s) 282, 401
Bsa29I ATCGAT 1 cut(s) 1705
BsaAI YACGTR 2 cut(s) 50, 455
BsaBI GATNNNNATC 1 cut(s) 492
BsaJI CCNNGG 3 cut(s) 281, 343, 1547
BsaWI WCCGGW 3 cut(s) 189, 640, 728
BsaXI ACNNNNNCTCC 8 cut(s) 164, 194, 340, 370, 655, 685, 1495, 1525
Bsc4I CCNNNNNNNGG 6 cut(s) 102, 300, 917, 1729, 1761, 1841
Bse118I RCCGGY 1 cut(s) 728
Bse1I ACTGG 3 cut(s) 408, 997, 1430
Bse21I CCTNAGG 2 cut(s) 1626, 1643
Bse3DI GCAATG 4 cut(s) 806, 929, 1014, 1695
Bse8I GATNNNNATC 1 cut(s) 492
BseBI CCWGG 5 cut(s) 24, 597, 822, 1163, 1179
BseCI ATCGAT 1 cut(s) 1705
BseDI CCNNGG 3 cut(s) 281, 343, 1547
BseGI GGATG 6 cut(s) 772, 802, 1102, 1174, 1434, 1770
BseJI GATNNNNATC 1 cut(s) 492
BseLI CCNNNNNNNGG 6 cut(s) 102, 300, 917, 1729, 1761, 1841
BseMI GCAATG 4 cut(s) 806, 929, 1014, 1695
BseMII CTCAG 6 cut(s) 535, 1102, 1443, 1617, 1657, 1662
BseNI ACTGG 3 cut(s) 408, 997, 1430
BsePI GCGCGC 1 cut(s) 276
BseRI GAGGAG 6 cut(s) 59, 62, 185, 211, 364, 490
BseSI GKGCMC 1 cut(s) 550
BseYI CCCAGC 4 cut(s) 272, 298, 906, 1722
BsgI GTGCAG 2 cut(s) 276, 621
Bsh1236I CGCG 2 cut(s) 278, 296
Bsh1285I CGRYCG 1 cut(s) 194
BshFI GGCC 2 cut(s) 293, 335
BshTI ACCGGT 1 cut(s) 728
BshVI ATCGAT 1 cut(s) 1705
BsiEI CGRYCG 1 cut(s) 194
BsiHKAI GWGCWC 1 cut(s) 550
BsiSI CCGG 6 cut(s) 190, 281, 326, 401, 641, 729
BslFI GGGAC 7 cut(s) 390, 622, 708, 723, 889, 1319, 1721
BslI CCNNNNNNNGG 6 cut(s) 102, 300, 917, 1729, 1761, 1841
BsmAI GTCTC 2 cut(s) 237, 452
BsmFI GGGAC 7 cut(s) 390, 622, 708, 723, 889, 1319, 1721
BsnI GGCC 2 cut(s) 293, 335
Bsp1286I GDGCHC 1 cut(s) 550
Bsp143I GATC 7 cut(s) 11, 487, 504, 517, 758, 1144, 1676
BspACI CCGC 5 cut(s) 177, 294, 308, 340, 1727
BspANI GGCC 2 cut(s) 293, 335
BspCNI CTCAG 6 cut(s) 534, 1101, 1444, 1618, 1656, 1663
BspDI ATCGAT 1 cut(s) 1705
BspFNI CGCG 2 cut(s) 278, 296
BspHI TCATGA 1 cut(s) 1201
BspLI GGNNCC 2 cut(s) 405, 1434
BspMAI CTGCAG 3 cut(s) 368, 886, 1573
BspMI ACCTGC 1 cut(s) 889
BspOI GCTAGC 1 cut(s) 1358
BspPI GGATC 2 cut(s) 19, 766
BsrDI GCAATG 4 cut(s) 806, 929, 1014, 1695
BsrFI RCCGGY 1 cut(s) 728
BsrI ACTGG 3 cut(s) 408, 997, 1430
BssAI RCCGGY 1 cut(s) 728
BssECI CCNNGG 3 cut(s) 281, 343, 1547
BssHII GCGCGC 1 cut(s) 276
BssMI GATC 7 cut(s) 11, 487, 504, 517, 758, 1144, 1676
Bst2UI CCWGG 5 cut(s) 24, 597, 822, 1163, 1179
Bst4CI ACNGT 3 cut(s) 254, 463, 722
Bst6I CTCTTC 3 cut(s) 80, 321, 324
BstBAI YACGTR 2 cut(s) 50, 455
BstC8I GCNNGC 5 cut(s) 278, 392, 1094, 1356, 1847
BstDEI CTNAG 8 cut(s) 521, 685, 894, 1088, 1452, 1626, 1643, 1671
BstDSI CCRYGG 1 cut(s) 1547
BstF5I GGATG 6 cut(s) 772, 802, 1102, 1174, 1434, 1770
BstFNI CGCG 2 cut(s) 278, 296
BstHHI GCGC 5 cut(s) 211, 278, 280, 298, 566
BstKTI GATC 7 cut(s) 14, 490, 507, 520, 761, 1147, 1679
BstMAI GTCTC 2 cut(s) 237, 452
BstMBI GATC 7 cut(s) 11, 487, 504, 517, 758, 1144, 1676
BstMCI CGRYCG 1 cut(s) 194
BstMWI GCNNNNNNNGC 4 cut(s) 223, 960, 1004, 1069
BstNI CCWGG 5 cut(s) 24, 597, 822, 1163, 1179
BstNSI RCATGY 3 cut(s) 394, 1394, 1849
BstSCI CCNGG 7 cut(s) 22, 280, 399, 595, 820, 1161, 1177
BstSFI CTRYAG 4 cut(s) 364, 882, 1067, 1569
BstSLI GKGCMC 1 cut(s) 550
BstUI CGCG 2 cut(s) 278, 296
BstV2I GAAGAC 2 cut(s) 824, 1816
BstX2I RGATCY 1 cut(s) 11
BstYI RGATCY 1 cut(s) 11
Bsu15I ATCGAT 1 cut(s) 1705
Bsu36I CCTNAGG 2 cut(s) 1626, 1643
BsuI GTATCC 1 cut(s) 1588
BsuRI GGCC 2 cut(s) 293, 335
BsuTUI ATCGAT 1 cut(s) 1705
BtgI CCRYGG 1 cut(s) 1547
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 6 cut(s) 772, 802, 1102, 1174, 1434, 1770
BtsI GCAGTG 2 cut(s) 767, 970
BtsIMutI CAGTG 4 cut(s) 415, 767, 970, 1830
BveI ACCTGC 1 cut(s) 889
Cac8I GCNNGC 5 cut(s) 278, 392, 1094, 1356, 1847
CaiI CAGNNNCTG 2 cut(s) 1232, 1650
CciI TCATGA 1 cut(s) 1201
CfoI GCGC 5 cut(s) 211, 278, 280, 298, 566
Cfr10I RCCGGY 1 cut(s) 728
Cfr13I GGNCC 1 cut(s) 26
ClaI ATCGAT 1 cut(s) 1705
Csp6I GTAC 4 cut(s) 452, 848, 1120, 1515
CspAI ACCGGT 1 cut(s) 728
CviQI GTAC 4 cut(s) 452, 848, 1120, 1515
DdeI CTNAG 8 cut(s) 521, 685, 894, 1088, 1452, 1626, 1643, 1671
DpnI GATC 7 cut(s) 13, 489, 506, 519, 760, 1146, 1678
DpnII GATC 7 cut(s) 11, 487, 504, 517, 758, 1144, 1676
DraI TTTAAA 2 cut(s) 1410, 1582
DraIII CACNNNGTG 1 cut(s) 845
DrdI GACNNNNNNGTC 1 cut(s) 466
DseDI GACNNNNNNGTC 1 cut(s) 466
Eam1104I CTCTTC 3 cut(s) 80, 321, 324
EarI CTCTTC 3 cut(s) 80, 321, 324
Eco47I GGWCC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 816
Eco81I CCTNAGG 2 cut(s) 1626, 1643
EcoO109I RGGNCCY 1 cut(s) 26
EcoRII CCWGG 5 cut(s) 22, 595, 820, 1161, 1177
FalI AAGNNNNNCTT 2 cut(s) 820, 852
FaqI GGGAC 7 cut(s) 390, 622, 708, 723, 889, 1319, 1721
FauI CCCGC 1 cut(s) 170
FokI GGATG 6 cut(s) 759, 789, 1089, 1161, 1441, 1777
FspBI CTAG 6 cut(s) 86, 471, 555, 573, 843, 1355
FspI TGCGCA 2 cut(s) 210, 565
GlaI GCGC 5 cut(s) 210, 277, 279, 297, 565
GsaI CCCAGC 4 cut(s) 276, 302, 910, 1726
GsuI CTGGAG 5 cut(s) 6, 843, 1014, 1184, 1419
HaeIII GGCC 2 cut(s) 293, 335
HapII CCGG 6 cut(s) 190, 281, 326, 401, 641, 729
HhaI GCGC 5 cut(s) 211, 278, 280, 298, 566
Hin6I GCGC 5 cut(s) 209, 276, 278, 296, 564
HinP1I GCGC 5 cut(s) 209, 276, 278, 296, 564
HindIII AAGCTT 1 cut(s) 224
HpaII CCGG 6 cut(s) 190, 281, 326, 401, 641, 729
HphI GGTGA 5 cut(s) 611, 756, 831, 849, 864
Hpy166II GTNNAC 4 cut(s) 500, 548, 848, 1533
Hpy188I TCNGA 5 cut(s) 289, 796, 1453, 1477, 1877
Hpy188III TCNNGA 6 cut(s) 618, 1031, 1202, 1386, 1436, 1610
Hpy8I GTNNAC 4 cut(s) 500, 548, 848, 1533
Hpy99I CGWCG 4 cut(s) 42, 118, 142, 145
HpyAV CCTTC 8 cut(s) 154, 406, 624, 691, 752, 846, 1006, 1556
HpyCH4III ACNGT 3 cut(s) 254, 463, 722
HpyCH4IV ACGT 4 cut(s) 49, 234, 454, 956
HpyF10VI GCNNNNNNNGC 4 cut(s) 223, 960, 1004, 1069
HpyF3I CTNAG 8 cut(s) 521, 685, 894, 1088, 1452, 1626, 1643, 1671
HpySE526I ACGT 4 cut(s) 49, 234, 454, 956
HspAI GCGC 5 cut(s) 209, 276, 278, 296, 564
Kzo9I GATC 7 cut(s) 11, 487, 504, 517, 758, 1144, 1676
LmnI GCTCC 7 cut(s) 136, 409, 824, 995, 1217, 1438, 1724
LweI GCATC 3 cut(s) 781, 1111, 1434
MaeI CTAG 6 cut(s) 86, 471, 555, 573, 843, 1355
MaeII ACGT 4 cut(s) 49, 234, 454, 956
MaeIII GTNAC 3 cut(s) 479, 787, 886
MalI GATC 7 cut(s) 13, 489, 506, 519, 760, 1146, 1678
MboI GATC 7 cut(s) 11, 487, 504, 517, 758, 1144, 1676
MfeI CAATTG 1 cut(s) 1850
MflI RGATCY 1 cut(s) 11
MhlI GDGCHC 1 cut(s) 550
MluCI AATT 4 cut(s) 1134, 1321, 1605, 1850
MlyI GAGTC 7 cut(s) 51, 83, 313, 604, 658, 911, 1561
MmeI TCCRAC 1 cut(s) 1817
MroXI GAANNNNTTC 1 cut(s) 1026
MseI TTAA 6 cut(s) 231, 915, 1188, 1409, 1581, 1590
MslI CAYNNNNRTG 2 cut(s) 1212, 1844
MspI CCGG 6 cut(s) 190, 281, 326, 401, 641, 729
MspR9I CCNGG 7 cut(s) 24, 282, 401, 597, 822, 1163, 1179
MunI CAATTG 1 cut(s) 1850
MvaI CCWGG 5 cut(s) 24, 597, 822, 1163, 1179
MvnI CGCG 2 cut(s) 278, 296
MwoI GCNNNNNNNGC 4 cut(s) 223, 960, 1004, 1069
NciI CCSGG 2 cut(s) 282, 401
NdeII GATC 7 cut(s) 11, 487, 504, 517, 758, 1144, 1676
NheI GCTAGC 1 cut(s) 1354
NlaIV GGNNCC 2 cut(s) 405, 1434
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 1 cut(s) 886
NsbI TGCGCA 2 cut(s) 210, 565
NspI RCATGY 3 cut(s) 394, 1394, 1849
PaeI GCATGC 2 cut(s) 394, 1849
PagI TCATGA 1 cut(s) 1201
PauI GCGCGC 1 cut(s) 276
PciI ACATGT 1 cut(s) 1390
PcsI WCGNNNNNNNCGW 3 cut(s) 46, 122, 149
PdmI GAANNNNTTC 1 cut(s) 1026
PfeI GAWTC 5 cut(s) 576, 1334, 1399, 1768, 1831
PflMI CCANNNNNTGG 1 cut(s) 102
PfoI TCCNGGA 2 cut(s) 22, 820
PinAI ACCGGT 1 cut(s) 728
PleI GAGTC 7 cut(s) 50, 83, 312, 604, 658, 910, 1560
PpsI GAGTC 7 cut(s) 50, 83, 312, 604, 658, 910, 1560
Ppu21I YACGTR 2 cut(s) 50, 455
PpuMI RGGWCCY 1 cut(s) 26
PscI ACATGT 1 cut(s) 1390
Psp5II RGGWCCY 1 cut(s) 26
Psp6I CCWGG 5 cut(s) 22, 595, 820, 1161, 1177
PspFI CCCAGC 4 cut(s) 272, 298, 906, 1722
PspGI CCWGG 5 cut(s) 22, 595, 820, 1161, 1177
PspN4I GGNNCC 2 cut(s) 405, 1434
PspPI GGNCC 1 cut(s) 26
PspPPI RGGWCCY 1 cut(s) 26
PsrI GAACNNNNNNTAC 2 cut(s) 691, 723
PstI CTGCAG 3 cut(s) 368, 886, 1573
PstNI CAGNNNCTG 2 cut(s) 1232, 1650
PsuI RGATCY 1 cut(s) 11
PteI GCGCGC 1 cut(s) 276
RsaI GTAC 4 cut(s) 453, 849, 1121, 1516
RsaNI GTAC 4 cut(s) 452, 848, 1120, 1515
RseI CAYNNNNRTG 2 cut(s) 1212, 1844
SaqAI TTAA 6 cut(s) 231, 915, 1188, 1409, 1581, 1590
Sau3AI GATC 7 cut(s) 11, 487, 504, 517, 758, 1144, 1676
Sau96I GGNCC 1 cut(s) 26
ScaI AGTACT 1 cut(s) 1121
SchI GAGTC 7 cut(s) 51, 83, 313, 604, 658, 911, 1561
ScrFI CCNGG 7 cut(s) 24, 282, 401, 597, 822, 1163, 1179
SduI GDGCHC 1 cut(s) 550
SfaNI GCATC 3 cut(s) 781, 1111, 1434
SfcI CTRYAG 4 cut(s) 364, 882, 1067, 1569
SinI GGWCC 1 cut(s) 26
SmiMI CAYNNNNRTG 2 cut(s) 1212, 1844
SmlI CTYRAG 1 cut(s) 1097
SmoI CTYRAG 1 cut(s) 1097
SpeI ACTAGT 1 cut(s) 554
SphI GCATGC 2 cut(s) 394, 1849
Sse9I AATT 4 cut(s) 1134, 1321, 1605, 1850
SsiI CCGC 5 cut(s) 177, 294, 308, 340, 1727
SspI AATATT 1 cut(s) 1111
SspMI CTAG 6 cut(s) 86, 471, 555, 573, 843, 1355
StyD4I CCNGG 7 cut(s) 22, 280, 399, 595, 820, 1161, 1177
TaaI ACNGT 3 cut(s) 254, 463, 722
TaiI ACGT 4 cut(s) 52, 237, 457, 959
TaqI TCGA 7 cut(s) 40, 194, 490, 528, 617, 745, 1705
TasI AATT 4 cut(s) 1134, 1321, 1605, 1850
TatI WGTACW 2 cut(s) 1119, 1514
TauI GCSGC 2 cut(s) 296, 1729
TfiI GAWTC 5 cut(s) 576, 1334, 1399, 1768, 1831
Tru1I TTAA 6 cut(s) 231, 915, 1188, 1409, 1581, 1590
Tru9I TTAA 6 cut(s) 231, 915, 1188, 1409, 1581, 1590
TscAI CASTG 4 cut(s) 415, 774, 970, 1830
TseFI GTSAC 1 cut(s) 886
Tsp45I GTSAC 1 cut(s) 886
TspGWI ACGGA 2 cut(s) 549, 1536
TspRI CASTG 4 cut(s) 415, 774, 970, 1830
Van91I CCANNNNNTGG 1 cut(s) 102
VneI GTGCAC 1 cut(s) 546
VpaK11BI GGWCC 1 cut(s) 26
XceI RCATGY 3 cut(s) 394, 1394, 1849
XcmI CCANNNNNNNNNTGG 1 cut(s) 1262
XmnI GAANNNNTTC 1 cut(s) 1026
XspI CTAG 6 cut(s) 86, 471, 555, 573, 843, 1355
ZrmI AGTACT 1 cut(s) 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.