Rmu_ssc0000158.1_g000006

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000158.1
OG0001271
Physical Location & Seq
Reverse (-)
36211 .. 42200
5990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000158.1_g000006.1.cds

Sequence Viewer

Length: 2622 bp
ctgatagtcggattcgagattccggtgactaattgtcggattccggtcactggacatcggactccggtcaccggtcgcctgagaccgcagccggccactgaaaataatacgaagaaccagaaggtcgtctcatgcattgacattgtaaacacacagcaacaaggaagagagacacgcgcccattcctcgctggctgacttggacctctccaatctcctctcatcatcaagatctcaaacagggggcattctgacattttcaaccgacggcttcaacaaaacccacagcagcaccaccagttgccaccgcctaccacttccacaaccttcttcttcaacctttcccaatcacctaatgccttctccatctcaatctcttcaaaaacgaaccttttttacatcttcatcttctactcctgcttcctcctcctcctctgtctcagctctgagacccgggatcaacagaagcggcagtgatgttaacagcccacggcggctcacccgccagaggaggctgcgctacctcacgagccccgaggatctcgactcttacggggtcccggagaagtctcgctcatcgcccacctcgcctgaatattcgacccgtagatcacgctctccgtgtggcgagtcaaacccaaattcacgctctcctcccggtggcgagtcggccccatgttctcggtcttcggtcggtgactcggcccgaaagtcatgctcctcttctttcgttgctgggcctcagggcccacaacccttgccgcttccggagaacaaccggagacccgactgcgtctccttcttcagccctgtggtcggcaggaagagcgtcgatcaagctgccaccagctcatcatcaccggcgaaatcttccaagaaccgatgccgagatgcaaacaattcctgcgggggtgggggttttaatttccatttgggtttgaatgttcaatctaggactactaggagtgccctacagactgtttttagctcaagtcatcatcccgttagcccacaaagatcaaacacagtggattttttgccttgtcagtcacatgttgcaggcaactattataacaggggaatgggaatggcagtgtcaccggaggctaactttgaacgtgaaacttacaacttgagcaggagtgctccaaccagtgttctctcaagtcctgcggttagcccgcgccgatcaaccactgtggatccttttccttttttcacgacccatcaagatcaatatcaggatggtgctacttttccctcgtttttgttaccaaacaagcctacactcagtcccactcattctccactccatagccccacgcctcgaagtccacgcttcagccccaatgcttcaaactatgagagtagtcttgccaatgctcacccgttgcctctgcctcctggagccgcagtgccagcacagccgccctctatcactatgcatcaaaataatgtagtaacaatgaaaggtcaatggcagaaggggaaacttatcggacggggtacctttggtagtgtttatcttgcaaccaaccgagaaactggggccttgtgtgcaatgaaggaagttgatctcattcctgatgatcctaaatctgcagaaagtataaagcaattggagcaggaaattaaagtcctccgtacactaaagcatccaaatattgtgcagtactatggtagtgaagtgattgatgatcatttctacatatatttagaatatgttcaccccggatcaataaataaatatgtccgagaccatattggagctatgactgaatccgtaatccgcaattttacccgccatatcctctgtgggttggcttttttgcatgacacgaagacaattcacagggatattaaaggtgcaaatttgctcgttgatgcatcaggcgttgtcaaacttgccgattttgggatggcaaaacatcttggtgcgcattcatacaatctttctttgaagggcagcccatactggatggctcctgaggtcataaaggctgtgatacagaatgatggtgatcctaatcttgctttggctgttgatatatggagcttgggttgtaccatcattgagatgttcaatggaaaaccgccttggagtgaatttgaagggccacaagctatgttcaaggttctgcacagaaccccggatataccagaaaaattatctgcagaaggaaaggaattcctcagtttgtgcttcaaaaggaaccccgcagagaggccatcagctaagaagctacttgatcatccttttgtacgaaattcaaatgatcaaaatgtctcatcttatactcgggctttttctctggtaatggataaattgaatagcccgacggaccatattaagcaaaaacttgatcgaatgctgcatgcagtaggatttagatttggaagcaggtggttatatgactatgatcaagttgagaattttcaactaacacggcagggagaggcgaggaaaccagtaaccgcgtaccgcctgaaccggcgagcagcaccacctgcaaacctctcgagggccaagtcagctaagtataagcataccgagtcagcttataattttcgatgtgggattctttcagagctctgcttcagcgttaaatggagcttccaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

873

Amino Acids

96.13

Weight (kDa)

9.6

Isoelectric Point (pI)

61.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1071, 2562
AarI CACCTGC 2 cut(s) 2391, 2515
Acc16I TGCGCA 1 cut(s) 1938
Acc36I ACCTGC 2 cut(s) 2391, 2515
Acc65I GGTACC 1 cut(s) 1505
AccB1I GGYRCC 1 cut(s) 1505
AccII CGCG 3 cut(s) 177, 1183, 2477
AccIII TCCGGA 1 cut(s) 766
AclWI GGATC 7 cut(s) 464, 546, 1196, 1209, 1583, 1741, 2015
AcoI YGGCCR 1 cut(s) 93
AcsI RAATTY 6 cut(s) 640, 1870, 2105, 2186, 2266, 2431
AcuI CTGAAG 3 cut(s) 787, 1324, 2581
AfaI GTAC 6 cut(s) 1507, 1645, 1673, 2065, 2262, 2480
AfiI CCNNNNNNNGG 8 cut(s) 50, 71, 507, 659, 815, 1914, 2223, 2521
AflIII ACRYGT 1 cut(s) 1051
AgeI ACCGGT 1 cut(s) 71
AhdI GACNNNNNGTC 1 cut(s) 33
AjnI CCWGG 1 cut(s) 1402
AjuI GAANNNNNNNTTGG 2 cut(s) 2080, 2112
Alw21I GWGCWC 2 cut(s) 1147, 2592
AlwI GGATC 7 cut(s) 464, 546, 1196, 1209, 1583, 1741, 2015
Ama87I CYCGRG 4 cut(s) 452, 533, 2298, 2518
Aor13HI TCCGGA 1 cut(s) 766
AoxI GGCC 9 cut(s) 93, 669, 702, 737, 745, 1548, 2114, 2225, 2523
ApaI GGGCCC 1 cut(s) 749
ApeKI GCWGC 7 cut(s) 88, 288, 514, 839, 1965, 2371, 2498
ApoI RAATTY 6 cut(s) 640, 1870, 2105, 2186, 2266, 2431
AsiGI ACCGGT 1 cut(s) 71
Asp700I GAANNNNTTC 1 cut(s) 1722
Asp718I GGTACC 1 cut(s) 1505
AspLEI GCGC 4 cut(s) 179, 519, 1185, 1939
AsuC2I CCSGG 6 cut(s) 453, 454, 560, 657, 1731, 2150
AvaI CYCGRG 4 cut(s) 452, 533, 2298, 2518
AvaII GGWCC 3 cut(s) 202, 556, 2341
AxyI CCTNAGG 2 cut(s) 741, 1986
BaeGI GKGCMC 2 cut(s) 749, 970
BamHI GGATCC 1 cut(s) 1201
BanI GGYRCC 1 cut(s) 1505
BanII GRGCYC 3 cut(s) 533, 749, 2592
BauI CACGAG 1 cut(s) 526
BbsI GAAGAC 2 cut(s) 678, 1847
Bbv12I GWGCWC 2 cut(s) 1147, 2592
BbvI GCAGC 7 cut(s) 100, 300, 501, 826, 1977, 2358, 2510
BccI CCATC 8 cut(s) 373, 1233, 1238, 1912, 1972, 2009, 2075, 2236
BceAI ACGGC 3 cut(s) 283, 506, 2462
BcgI CGANNNNNNTGC 6 cut(s) 696, 730, 821, 855, 2499, 2533
BciT130I CCWGG 1 cut(s) 1404
BclI TGATCA 4 cut(s) 1696, 2248, 2275, 2419
BcnI CCSGG 6 cut(s) 453, 454, 560, 657, 1731, 2150
BfaI CTAG 2 cut(s) 951, 960
BfmI CTRYAG 3 cut(s) 971, 1599, 2172
BfuAI ACCTGC 2 cut(s) 2391, 2515
BglII AGATCT 1 cut(s) 230
BmcAI AGTACT 1 cut(s) 1673
Bme1390I CCNGG 7 cut(s) 453, 454, 560, 657, 1404, 1731, 2150
Bme18I GGWCC 3 cut(s) 202, 556, 2341
BmeRI GACNNNNNGTC 1 cut(s) 33
BmeT110I CYCGRG 4 cut(s) 452, 533, 2298, 2518
BmrFI CCNGG 7 cut(s) 453, 454, 560, 657, 1404, 1731, 2150
BmrI ACTGGG 1 cut(s) 1554
BmsI GCATC 6 cut(s) 872, 880, 1453, 1663, 1873, 1895
BmuI ACTGGG 1 cut(s) 1554
BoxI GACNNNNGTC 1 cut(s) 65
BpiI GAAGAC 2 cut(s) 678, 1847
BpmI CTGGAG 1 cut(s) 1425
BpuEI CTTGAG 3 cut(s) 973, 1147, 1153
BpuMI CCSGG 6 cut(s) 453, 454, 560, 657, 1731, 2150
BsaBI GATNNNNATC 3 cut(s) 1236, 2019, 2025
BsaI GGTCTC 4 cut(s) 76, 442, 775, 1749
BsaJI CCNNGG 6 cut(s) 452, 488, 534, 1729, 2096, 2148
BsaWI WCCGGW 7 cut(s) 22, 43, 64, 71, 766, 777, 1099
BsaXI ACNNNNNCTCC 8 cut(s) 779, 809, 1288, 1318, 1398, 1428, 2044, 2074
Bsc4I CCNNNNNNNGG 8 cut(s) 50, 71, 507, 659, 815, 1914, 2223, 2521
Bse118I RCCGGY 4 cut(s) 71, 91, 859, 2490
Bse1I ACTGG 6 cut(s) 55, 297, 1152, 1549, 1979, 2468
Bse21I CCTNAGG 2 cut(s) 741, 1986
Bse3DI GCAATG 1 cut(s) 1566
Bse8I GATNNNNATC 3 cut(s) 1236, 2019, 2025
BseAI TCCGGA 1 cut(s) 766
BseBI CCWGG 1 cut(s) 1404
BseDI CCNNGG 6 cut(s) 452, 488, 534, 1729, 2096, 2148
BseGI GGATG 6 cut(s) 997, 1249, 1654, 1923, 1983, 2251
BseJI GATNNNNATC 3 cut(s) 1236, 2019, 2025
BseLI CCNNNNNNNGG 8 cut(s) 50, 71, 507, 659, 815, 1914, 2223, 2521
BseMI GCAATG 1 cut(s) 1566
BseMII CTCAG 7 cut(s) 71, 437, 453, 755, 1303, 1977, 2206
BseNI ACTGG 6 cut(s) 55, 297, 1152, 1549, 1979, 2468
BseRI GAGGAG 7 cut(s) 206, 415, 418, 421, 523, 642, 709
BseSI GKGCMC 2 cut(s) 749, 970
BseXI GCAGC 7 cut(s) 100, 300, 501, 826, 1977, 2358, 2510
BseYI CCCAGC 1 cut(s) 734
BsgI GTGCAG 2 cut(s) 1688, 2123
Bsh1236I CGCG 3 cut(s) 177, 1183, 2477
Bsh1285I CGRYCG 2 cut(s) 76, 693
BshFI GGCC 9 cut(s) 95, 671, 704, 739, 747, 1550, 2116, 2227, 2525
BshNI GGYRCC 1 cut(s) 1505
BshTI ACCGGT 1 cut(s) 71
BsiEI CGRYCG 2 cut(s) 76, 693
BsiHKAI GWGCWC 2 cut(s) 1147, 2592
BsiHKCI CYCGRG 4 cut(s) 452, 533, 2298, 2518
BslFI GGGAC 2 cut(s) 542, 1278
BslI CCNNNNNNNGG 8 cut(s) 50, 71, 507, 659, 815, 1914, 2223, 2521
BsmBI CGTCTC 2 cut(s) 133, 799
BsmFI GGGAC 2 cut(s) 542, 1278
BsmI GAATGC 3 cut(s) 246, 1939, 2373
BsnI GGCC 9 cut(s) 95, 671, 704, 739, 747, 1550, 2116, 2227, 2525
Bso31I GGTCTC 4 cut(s) 76, 442, 775, 1749
BsoBI CYCGRG 4 cut(s) 452, 533, 2298, 2518
Bsp120I GGGCCC 1 cut(s) 745
Bsp1286I GDGCHC 5 cut(s) 533, 749, 970, 1147, 2592
Bsp13I TCCGGA 1 cut(s) 766
BspANI GGCC 9 cut(s) 95, 671, 704, 739, 747, 1550, 2116, 2227, 2525
BspCNI CTCAG 7 cut(s) 72, 438, 452, 754, 1302, 1978, 2205
BspEI TCCGGA 1 cut(s) 766
BspFNI CGCG 3 cut(s) 177, 1183, 2477
BspMAI CTGCAG 2 cut(s) 1603, 2176
BspMI ACCTGC 2 cut(s) 2391, 2515
BspPI GGATC 7 cut(s) 464, 546, 1196, 1209, 1583, 1741, 2015
BspQI GCTCTTC 1 cut(s) 818
BspT107I GGYRCC 1 cut(s) 1505
BspTNI GGTCTC 4 cut(s) 76, 442, 775, 1749
BsrDI GCAATG 1 cut(s) 1566
BsrFI RCCGGY 4 cut(s) 71, 91, 859, 2490
BsrI ACTGG 6 cut(s) 55, 297, 1152, 1549, 1979, 2468
BssAI RCCGGY 4 cut(s) 71, 91, 859, 2490
BssECI CCNNGG 6 cut(s) 452, 488, 534, 1729, 2096, 2148
BssSI CACGAG 1 cut(s) 526
BssT1I CCWWGG 1 cut(s) 2096
Bst2BI CACGAG 1 cut(s) 526
Bst2UI CCWGG 1 cut(s) 1404
Bst4CI ACNGT 3 cut(s) 979, 1027, 1198
Bst6I CTCTTC 4 cut(s) 160, 381, 727, 818
BstAPI GCANNNNNTGC 1 cut(s) 2507
BstC8I GCNNGC 7 cut(s) 93, 192, 1060, 1181, 1419, 2376, 2496
BstDEI CTNAG 9 cut(s) 80, 439, 446, 741, 1289, 1986, 2192, 2235, 2535
BstDSI CCRYGG 1 cut(s) 488
BstEII GGTNACC 1 cut(s) 67
BstF5I GGATG 6 cut(s) 997, 1249, 1654, 1923, 1983, 2251
BstFNI CGCG 3 cut(s) 177, 1183, 2477
BstHHI GCGC 4 cut(s) 179, 519, 1185, 1939
BstMCI CGRYCG 2 cut(s) 76, 693
BstMWI GCNNNNNNNGC 8 cut(s) 586, 825, 1418, 1423, 1556, 1621, 2507, 2531
BstNI CCWGG 1 cut(s) 1404
BstNSI RCATGY 2 cut(s) 1055, 2378
BstPAI GACNNNNGTC 1 cut(s) 65
BstPI GGTNACC 1 cut(s) 67
BstSCI CCNGG 7 cut(s) 451, 452, 558, 655, 1402, 1729, 2148
BstSFI CTRYAG 3 cut(s) 971, 1599, 2172
BstSLI GKGCMC 2 cut(s) 749, 970
BstUI CGCG 3 cut(s) 177, 1183, 2477
BstV1I GCAGC 7 cut(s) 100, 300, 501, 826, 1977, 2358, 2510
BstV2I GAAGAC 2 cut(s) 678, 1847
BstX2I RGATCY 3 cut(s) 230, 538, 1201
BstYI RGATCY 3 cut(s) 230, 538, 1201
Bsu36I CCTNAGG 2 cut(s) 741, 1986
BsuRI GGCC 9 cut(s) 95, 671, 704, 739, 747, 1550, 2116, 2227, 2525
BtgI CCRYGG 1 cut(s) 488
BtgZI GCGATG 1 cut(s) 561
BtsCI GGATG 6 cut(s) 997, 1249, 1654, 1923, 1983, 2251
BtsI GCAGTG 3 cut(s) 478, 1098, 1419
BtsIMutI CAGTG 8 cut(s) 48, 96, 478, 1032, 1098, 1159, 1194, 1419
BveI ACCTGC 2 cut(s) 2391, 2515
Cac8I GCNNGC 7 cut(s) 93, 192, 1060, 1181, 1419, 2376, 2496
CfoI GCGC 4 cut(s) 179, 519, 1185, 1939
Cfr10I RCCGGY 4 cut(s) 71, 91, 859, 2490
Cfr9I CCCGGG 1 cut(s) 452
CseI GACGC 2 cut(s) 781, 817
Csp6I GTAC 6 cut(s) 1506, 1644, 1672, 2064, 2261, 2479
CspAI ACCGGT 1 cut(s) 71
CspCI CAANNNNNGTGG 6 cut(s) 738, 773, 1008, 1043, 1179, 1214
CviAII CATG 6 cut(s) 132, 675, 714, 1052, 1832, 2375
CviQI GTAC 6 cut(s) 1506, 1644, 1672, 2064, 2261, 2479
DdeI CTNAG 9 cut(s) 80, 439, 446, 741, 1289, 1986, 2192, 2235, 2535
DriI GACNNNNNGTC 1 cut(s) 33
EaeI YGGCCR 1 cut(s) 93
Eam1104I CTCTTC 4 cut(s) 160, 381, 727, 818
Eam1105I GACNNNNNGTC 1 cut(s) 33
EarI CTCTTC 4 cut(s) 160, 381, 727, 818
Ecl136II GAGCTC 1 cut(s) 2590
Eco130I CCWWGG 1 cut(s) 2096
Eco24I GRGCYC 3 cut(s) 533, 749, 2592
Eco31I GGTCTC 4 cut(s) 76, 442, 775, 1749
Eco47I GGWCC 3 cut(s) 202, 556, 2341
Eco53kI GAGCTC 1 cut(s) 2590
Eco57I CTGAAG 3 cut(s) 787, 1324, 2581
Eco81I CCTNAGG 2 cut(s) 741, 1986
Eco88I CYCGRG 4 cut(s) 452, 533, 2298, 2518
Eco91I GGTNACC 1 cut(s) 67
EcoICRI GAGCTC 1 cut(s) 2590
EcoO109I RGGNCCY 3 cut(s) 556, 745, 1548
EcoO65I GGTNACC 1 cut(s) 67
EcoRI GAATTC 1 cut(s) 2186
EcoRII CCWGG 1 cut(s) 1402
EcoT14I CCWWGG 1 cut(s) 2096
EcoT22I ATGCAT 3 cut(s) 137, 1446, 1888
EcoT38I GRGCYC 3 cut(s) 533, 749, 2592
ErhI CCWWGG 1 cut(s) 2096
Esp3I CGTCTC 2 cut(s) 133, 799
FaeI CATG 6 cut(s) 135, 678, 717, 1055, 1835, 2378
FaqI GGGAC 2 cut(s) 542, 1278
FatI CATG 6 cut(s) 131, 674, 713, 1051, 1831, 2374
FauI CCCGC 5 cut(s) 509, 899, 1188, 1808, 2224
FbaI TGATCA 4 cut(s) 1696, 2248, 2275, 2419
FokI GGATG 6 cut(s) 984, 1256, 1641, 1930, 1990, 2238
FriOI GRGCYC 3 cut(s) 533, 749, 2592
FspAI RTGCGCAY 1 cut(s) 1938
FspBI CTAG 2 cut(s) 951, 960
FspI TGCGCA 1 cut(s) 1938
GlaI GCGC 4 cut(s) 178, 518, 1184, 1938
GsaI CCCAGC 1 cut(s) 738
GsuI CTGGAG 1 cut(s) 1425
HaeIII GGCC 9 cut(s) 95, 671, 704, 739, 747, 1550, 2116, 2227, 2525
HgaI GACGC 2 cut(s) 781, 817
HhaI GCGC 4 cut(s) 179, 519, 1185, 1939
Hin1II CATG 6 cut(s) 135, 678, 717, 1055, 1835, 2378
Hin6I GCGC 4 cut(s) 177, 517, 1183, 1937
HinP1I GCGC 4 cut(s) 177, 517, 1183, 1937
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HpaI GTTAAC 1 cut(s) 481
Hpy166II GTNNAC 5 cut(s) 148, 481, 1334, 1646, 1726
Hpy188I TCNGA 8 cut(s) 11, 39, 60, 252, 447, 1499, 1754, 2587
Hpy8I GTNNAC 5 cut(s) 148, 481, 1334, 1646, 1726
Hpy99I CGWCG 3 cut(s) 269, 833, 2341
HpyAV CCTTC 9 cut(s) 115, 336, 369, 808, 1477, 1558, 1954, 2105, 2171
HpyCH4III ACNGT 3 cut(s) 979, 1027, 1198
HpyCH4IV ACGT 1 cut(s) 1117
HpyF10VI GCNNNNNNNGC 8 cut(s) 586, 825, 1418, 1423, 1556, 1621, 2507, 2531
HpyF3I CTNAG 9 cut(s) 80, 439, 446, 741, 1289, 1986, 2192, 2235, 2535
HpySE526I ACGT 1 cut(s) 1117
Hsp92II CATG 6 cut(s) 135, 678, 717, 1055, 1835, 2378
HspAI GCGC 4 cut(s) 177, 517, 1183, 1937
KflI GGGWCCC 1 cut(s) 556
Kpn2I TCCGGA 1 cut(s) 766
KpnI GGTACC 1 cut(s) 1509
KroI GCCGGC 1 cut(s) 91
KroNI GCCGGC 1 cut(s) 93
Ksp22I TGATCA 4 cut(s) 1696, 2248, 2275, 2419
KspAI GTTAAC 1 cut(s) 481
LguI GCTCTTC 1 cut(s) 818
LmnI GCTCC 8 cut(s) 722, 1150, 1406, 1621, 1766, 1987, 2052, 2610
Lsp1109I GCAGC 7 cut(s) 100, 300, 501, 826, 1977, 2358, 2510
LweI GCATC 6 cut(s) 872, 880, 1453, 1663, 1873, 1895
MaeI CTAG 2 cut(s) 951, 960
MaeII ACGT 1 cut(s) 1117
MaeIII GTNAC 9 cut(s) 25, 46, 67, 695, 1047, 1095, 1269, 1459, 2470
MfeI CAATTG 1 cut(s) 1616
MflI RGATCY 3 cut(s) 230, 538, 1201
MhlI GDGCHC 5 cut(s) 533, 749, 970, 1147, 2592
MlyI GAGTC 6 cut(s) 55, 539, 638, 674, 692, 2561
MmeI TCCRAC 2 cut(s) 17, 1172
Mph1103I ATGCAT 3 cut(s) 137, 1446, 1888
MroI TCCGGA 1 cut(s) 766
MroNI GCCGGC 1 cut(s) 91
MroXI GAANNNNTTC 1 cut(s) 1722
MseI TTAA 6 cut(s) 480, 921, 1632, 1860, 2349, 2604
MslI CAYNNNNRTG 2 cut(s) 1932, 2075
MspR9I CCNGG 7 cut(s) 453, 454, 560, 657, 1404, 1731, 2150
MunI CAATTG 1 cut(s) 1616
Mva1269I GAATGC 3 cut(s) 246, 1939, 2373
MvaI CCWGG 1 cut(s) 1404
MvnI CGCG 3 cut(s) 177, 1183, 2477
MwoI GCNNNNNNNGC 8 cut(s) 586, 825, 1418, 1423, 1556, 1621, 2507, 2531
NaeI GCCGGC 1 cut(s) 93
NciI CCSGG 6 cut(s) 453, 454, 560, 657, 1731, 2150
NgoMIV GCCGGC 1 cut(s) 91
NlaIII CATG 6 cut(s) 135, 678, 717, 1055, 1835, 2378
NmeAIII GCCGAG 2 cut(s) 680, 911
NmuCI GTSAC 6 cut(s) 25, 46, 67, 695, 1047, 1095
NsbI TGCGCA 1 cut(s) 1938
NsiI ATGCAT 3 cut(s) 137, 1446, 1888
NspI RCATGY 2 cut(s) 1055, 2378
PaeI GCATGC 1 cut(s) 2378
PaeR7I CTCGAG 1 cut(s) 2518
PaqCI CACCTGC 2 cut(s) 2391, 2515
PciI ACATGT 1 cut(s) 1051
PciSI GCTCTTC 1 cut(s) 818
PctI GAATGC 3 cut(s) 246, 1939, 2373
PdiI GCCGGC 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 1722
PfeI GAWTC 5 cut(s) 12, 19, 40, 1778, 2578
PflFI GACNNNGTC 1 cut(s) 791
PfoI TCCNGGA 2 cut(s) 558, 1402
PinAI ACCGGT 1 cut(s) 71
PleI GAGTC 6 cut(s) 55, 539, 637, 673, 692, 2560
PpsI GAGTC 6 cut(s) 55, 539, 637, 673, 692, 2560
PpuMI RGGWCCY 1 cut(s) 556
PscI ACATGT 1 cut(s) 1051
PshAI GACNNNNGTC 1 cut(s) 65
PsiI TTATAA 2 cut(s) 1071, 2562
Psp124BI GAGCTC 1 cut(s) 2592
Psp5II RGGWCCY 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 1402
PspEI GGTNACC 1 cut(s) 67
PspFI CCCAGC 1 cut(s) 734
PspGI CCWGG 1 cut(s) 1402
PspOMI GGGCCC 1 cut(s) 745
PspPPI RGGWCCY 1 cut(s) 556
PsrI GAACNNNNNNTAC 2 cut(s) 379, 411
PstI CTGCAG 2 cut(s) 1603, 2176
PsuI RGATCY 3 cut(s) 230, 538, 1201
PsyI GACNNNGTC 1 cut(s) 791
RsaI GTAC 6 cut(s) 1507, 1645, 1673, 2065, 2262, 2480
RsaNI GTAC 6 cut(s) 1506, 1644, 1672, 2064, 2261, 2479
RseI CAYNNNNRTG 2 cut(s) 1932, 2075
SacI GAGCTC 1 cut(s) 2592
SapI GCTCTTC 1 cut(s) 818
SaqAI TTAA 6 cut(s) 480, 921, 1632, 1860, 2349, 2604
ScaI AGTACT 1 cut(s) 1673
SchI GAGTC 6 cut(s) 55, 539, 638, 674, 692, 2561
ScrFI CCNGG 7 cut(s) 453, 454, 560, 657, 1404, 1731, 2150
SduI GDGCHC 5 cut(s) 533, 749, 970, 1147, 2592
SfaNI GCATC 6 cut(s) 872, 880, 1453, 1663, 1873, 1895
SfcI CTRYAG 3 cut(s) 971, 1599, 2172
Sfr274I CTCGAG 1 cut(s) 2518
SgrAI CRCCGGYG 1 cut(s) 859
SinI GGWCC 3 cut(s) 202, 556, 2341
SlaI CTCGAG 1 cut(s) 2518
SmaI CCCGGG 1 cut(s) 454
SmiMI CAYNNNNRTG 2 cut(s) 1932, 2075
SmlI CTYRAG 4 cut(s) 988, 1132, 1162, 2518
SmoI CTYRAG 4 cut(s) 988, 1132, 1162, 2518
SphI GCATGC 1 cut(s) 2378
SspI AATATT 2 cut(s) 596, 1663
SspMI CTAG 2 cut(s) 951, 960
SstI GAGCTC 1 cut(s) 2592
StyD4I CCNGG 7 cut(s) 451, 452, 558, 655, 1402, 1729, 2148
StyI CCWWGG 1 cut(s) 2096
TaaI ACNGT 3 cut(s) 979, 1027, 1198
TaiI ACGT 1 cut(s) 1120
TaqI TCGA 8 cut(s) 15, 543, 599, 831, 1327, 2365, 2519, 2569
TaqII GACCGA 2 cut(s) 672, 679
TatI WGTACW 1 cut(s) 1671
TauI GCSGC 5 cut(s) 471, 496, 763, 1412, 1429
TfiI GAWTC 5 cut(s) 12, 19, 40, 1778, 2578
Tru1I TTAA 6 cut(s) 480, 921, 1632, 1860, 2349, 2604
Tru9I TTAA 6 cut(s) 480, 921, 1632, 1860, 2349, 2604
TscAI CASTG 8 cut(s) 55, 103, 478, 1032, 1098, 1159, 1201, 1419
TseFI GTSAC 6 cut(s) 25, 46, 67, 695, 1047, 1095
TseI GCWGC 7 cut(s) 88, 288, 514, 839, 1965, 2371, 2498
Tsp45I GTSAC 6 cut(s) 25, 46, 67, 695, 1047, 1095
TspDTI ATGAA 4 cut(s) 393, 1481, 1577, 1932
TspGWI ACGGA 4 cut(s) 609, 1631, 1771, 2354
TspMI CCCGGG 1 cut(s) 452
TspRI CASTG 8 cut(s) 55, 103, 478, 1032, 1098, 1159, 1201, 1419
Tth111I GACNNNGTC 1 cut(s) 791
VpaK11BI GGWCC 3 cut(s) 202, 556, 2341
XapI RAATTY 6 cut(s) 640, 1870, 2105, 2186, 2266, 2431
XceI RCATGY 2 cut(s) 1055, 2378
XcmI CCANNNNNNNNNTGG 1 cut(s) 1811
XhoI CTCGAG 1 cut(s) 2518
XmaI CCCGGG 1 cut(s) 452
XmnI GAANNNNTTC 1 cut(s) 1722
XspI CTAG 2 cut(s) 951, 960
ZrmI AGTACT 1 cut(s) 1673
Zsp2I ATGCAT 3 cut(s) 137, 1446, 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.