Rmu_co8435047.1_g000001

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8435047.1
OG0001271
Physical Location & Seq
Reverse (-)
1 .. 1156
1156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8435047.1_g000001.1.cds

Sequence Viewer

Length: 1030 bp
atgactcattttgatatatttgaagggggcattctgacattttcaaccgacggcttcaacaaaacccacagcagcaccaccagttgccaccgcctaccacttccacaaccttcttcttcaacctttcccaatcacctaatgccttctccatctcaatctcttcaaaaacgaaccttttttacatcttcatcttctactcctgcttcctcctcctcctctgtctcagctctgagacccgggatcaacagaagcggcagtgatgttaacagcccacggcggctcacccgccagaggaggctgcgctacctcacgagccccgaggatctcgactcttacggggtcccggagaagtctcgctcatcgcccacctcgcctgaatattcgacccgtagatcacgctctccgtgtggcgagtcaaacccaaattcacgctctcctcccggtggcgagtcggccccatgttctcggtcttcggtcggtgactcggcccgaaagtcatgctcctcttctttcgttgctgggcctcagggcccacaacccttgccgcttccggagaacaaccggagacccgactgcgtctccttcttcagccctgtggtcggcaggaagagcgtcgatcaagctgccaccagctcatcatcaccggcgaaatcttccaagaaccgatgccgagatgcaaacaattcctgcgggggtgggggttttaatttccatttgggtttgaatgttcaatctaggactactaggagtgccctacagactgtttttagctcaagtcatcatcccgttagcccacaaagatcaaacacagtggattttttgccttgtcagtcacatgttgcaggcaactattataacaggggaatgggaatggcagtgtcaccggaggctaactttgaacgtgaaacttacaacttgagcaggagtgctccaaccagtgttctctcaagtcctgcggttagcccgcgccgatcaaccactgtggatccttttccttttttcacgacccatcaagatcaatatcaggatg
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

343

Amino Acids

37.06

Weight (kDa)

9.85

Isoelectric Point (pI)

97.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 855
AccII CGCG 1 cut(s) 967
AccIII TCCGGA 1 cut(s) 550
AciI CCGC 8 cut(s) 91, 252, 277, 286, 545, 690, 956, 965
AclWI GGATC 4 cut(s) 248, 330, 980, 993
AcsI RAATTY 1 cut(s) 424
AcuI CTGAAG 1 cut(s) 571
AfiI CCNNNNNNNGG 3 cut(s) 291, 443, 599
AflIII ACRYGT 1 cut(s) 835
AgsI TTSAA 8 cut(s) 23, 45, 58, 120, 164, 724, 731, 899
AluBI AGCT 4 cut(s) 227, 623, 633, 771
AluI AGCT 4 cut(s) 227, 623, 633, 771
Alw21I GWGCWC 1 cut(s) 931
Alw26I GTCTC 5 cut(s) 226, 226, 357, 559, 583
AlwI GGATC 4 cut(s) 248, 330, 980, 993
Ama87I CYCGRG 2 cut(s) 236, 317
Aor13HI TCCGGA 1 cut(s) 550
AoxI GGCC 4 cut(s) 453, 486, 521, 529
ApaI GGGCCC 1 cut(s) 533
ApeKI GCWGC 3 cut(s) 72, 298, 623
ApoI RAATTY 1 cut(s) 424
AspLEI GCGC 2 cut(s) 303, 969
AspS9I GGNCC 6 cut(s) 340, 454, 487, 521, 529, 530
AsuC2I CCSGG 4 cut(s) 237, 238, 344, 441
AsuHPI GGTGA 5 cut(s) 125, 274, 491, 633, 873
AvaI CYCGRG 2 cut(s) 236, 317
AvaII GGWCC 1 cut(s) 340
AxyI CCTNAGG 1 cut(s) 525
BaeGI GKGCMC 2 cut(s) 533, 754
BamHI GGATCC 1 cut(s) 985
BanII GRGCYC 2 cut(s) 317, 533
BauI CACGAG 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 931
BbvI GCAGC 3 cut(s) 84, 285, 610
BccI CCATC 2 cut(s) 157, 1017
BceAI ACGGC 2 cut(s) 67, 290
BcgI CGANNNNNNTGC 4 cut(s) 480, 514, 605, 639
BcnI CCSGG 4 cut(s) 237, 238, 344, 441
BcoDI GTCTC 5 cut(s) 226, 226, 357, 559, 583
BfaI CTAG 2 cut(s) 735, 744
BfmI CTRYAG 1 cut(s) 755
BisI GCNGC 6 cut(s) 73, 253, 278, 299, 545, 624
BlsI GCNGC 6 cut(s) 74, 254, 279, 300, 546, 625
Bme1390I CCNGG 4 cut(s) 237, 238, 344, 441
Bme18I GGWCC 1 cut(s) 340
BmeT110I CYCGRG 2 cut(s) 236, 317
BmgT120I GGNCC 6 cut(s) 340, 454, 487, 521, 529, 530
BmiI GGNNCC 5 cut(s) 341, 342, 456, 531, 987
BmrFI CCNGG 4 cut(s) 237, 238, 344, 441
BmsI GCATC 2 cut(s) 656, 664
BpiI GAAGAC 1 cut(s) 462
BpuEI CTTGAG 3 cut(s) 757, 931, 937
BpuMI CCSGG 4 cut(s) 237, 238, 344, 441
BsaBI GATNNNNATC 1 cut(s) 1020
BsaI GGTCTC 2 cut(s) 226, 559
BsaJI CCNNGG 3 cut(s) 236, 272, 318
BsaWI WCCGGW 3 cut(s) 550, 561, 883
BsaXI ACNNNNNCTCC 2 cut(s) 563, 593
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 443, 599
Bse118I RCCGGY 1 cut(s) 643
Bse1I ACTGG 2 cut(s) 81, 936
Bse21I CCTNAGG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 1020
BseAI TCCGGA 1 cut(s) 550
BseDI CCNNGG 3 cut(s) 236, 272, 318
BseGI GGATG 1 cut(s) 781
BseJI GATNNNNATC 1 cut(s) 1020
BseLI CCNNNNNNNGG 3 cut(s) 291, 443, 599
BseMII CTCAG 3 cut(s) 221, 237, 539
BseNI ACTGG 2 cut(s) 81, 936
BseRI GAGGAG 6 cut(s) 199, 202, 205, 307, 426, 493
BseSI GKGCMC 2 cut(s) 533, 754
BseXI GCAGC 3 cut(s) 84, 285, 610
BseYI CCCAGC 1 cut(s) 518
Bsh1236I CGCG 1 cut(s) 967
Bsh1285I CGRYCG 1 cut(s) 477
BshFI GGCC 4 cut(s) 455, 488, 523, 531
BsiEI CGRYCG 1 cut(s) 477
BsiHKAI GWGCWC 1 cut(s) 931
BsiHKCI CYCGRG 2 cut(s) 236, 317
BsiSI CCGG 7 cut(s) 237, 344, 441, 551, 562, 644, 884
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 3 cut(s) 291, 443, 599
BsmAI GTCTC 5 cut(s) 226, 226, 357, 559, 583
BsmBI CGTCTC 1 cut(s) 583
BsmFI GGGAC 1 cut(s) 326
BsmI GAATGC 1 cut(s) 30
BsnI GGCC 4 cut(s) 455, 488, 523, 531
Bso31I GGTCTC 2 cut(s) 226, 559
BsoBI CYCGRG 2 cut(s) 236, 317
Bsp120I GGGCCC 1 cut(s) 529
Bsp1286I GDGCHC 4 cut(s) 317, 533, 754, 931
Bsp13I TCCGGA 1 cut(s) 550
Bsp143I GATC 8 cut(s) 240, 322, 392, 616, 800, 971, 985, 1015
BspACI CCGC 8 cut(s) 91, 252, 277, 286, 545, 690, 956, 965
BspANI GGCC 4 cut(s) 455, 488, 523, 531
BspCNI CTCAG 3 cut(s) 222, 236, 538
BspEI TCCGGA 1 cut(s) 550
BspFNI CGCG 1 cut(s) 967
BspLI GGNNCC 5 cut(s) 341, 342, 456, 531, 987
BspPI GGATC 4 cut(s) 248, 330, 980, 993
BspQI GCTCTTC 1 cut(s) 602
BspTNI GGTCTC 2 cut(s) 226, 559
BsrFI RCCGGY 1 cut(s) 643
BsrI ACTGG 2 cut(s) 81, 936
BssAI RCCGGY 1 cut(s) 643
BssECI CCNNGG 3 cut(s) 236, 272, 318
BssMI GATC 8 cut(s) 240, 322, 392, 616, 800, 971, 985, 1015
BssSI CACGAG 1 cut(s) 310
Bst2BI CACGAG 1 cut(s) 310
Bst4CI ACNGT 3 cut(s) 763, 811, 982
Bst6I CTCTTC 3 cut(s) 165, 511, 602
BstC8I GCNNGC 2 cut(s) 844, 965
BstDEI CTNAG 3 cut(s) 223, 230, 525
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 1 cut(s) 781
BstFNI CGCG 1 cut(s) 967
BstHHI GCGC 2 cut(s) 303, 969
BstKTI GATC 8 cut(s) 243, 325, 395, 619, 803, 974, 988, 1018
BstMAI GTCTC 5 cut(s) 226, 226, 357, 559, 583
BstMBI GATC 8 cut(s) 240, 322, 392, 616, 800, 971, 985, 1015
BstMCI CGRYCG 1 cut(s) 477
BstMWI GCNNNNNNNGC 2 cut(s) 370, 609
BstNSI RCATGY 1 cut(s) 839
BstSCI CCNGG 4 cut(s) 235, 236, 342, 439
BstSFI CTRYAG 1 cut(s) 755
BstSLI GKGCMC 2 cut(s) 533, 754
BstUI CGCG 1 cut(s) 967
BstV1I GCAGC 3 cut(s) 84, 285, 610
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 2 cut(s) 322, 985
BstYI RGATCY 2 cut(s) 322, 985
Bsu36I CCTNAGG 1 cut(s) 525
BsuRI GGCC 4 cut(s) 455, 488, 523, 531
BtgI CCRYGG 1 cut(s) 272
BtgZI GCGATG 1 cut(s) 345
BtsCI GGATG 1 cut(s) 781
BtsI GCAGTG 2 cut(s) 262, 882
BtsIMutI CAGTG 5 cut(s) 262, 816, 882, 943, 978
Cac8I GCNNGC 2 cut(s) 844, 965
CfoI GCGC 2 cut(s) 303, 969
Cfr10I RCCGGY 1 cut(s) 643
Cfr13I GGNCC 6 cut(s) 340, 454, 487, 521, 529, 530
Cfr9I CCCGGG 1 cut(s) 236
CseI GACGC 2 cut(s) 565, 601
CspCI CAANNNNNGTGG 6 cut(s) 522, 557, 792, 827, 963, 998
CviAII CATG 3 cut(s) 459, 498, 836
DdeI CTNAG 3 cut(s) 223, 230, 525
DpnI GATC 8 cut(s) 242, 324, 394, 618, 802, 973, 987, 1017
DpnII GATC 8 cut(s) 240, 322, 392, 616, 800, 971, 985, 1015
Eam1104I CTCTTC 3 cut(s) 165, 511, 602
EarI CTCTTC 3 cut(s) 165, 511, 602
Eco24I GRGCYC 2 cut(s) 317, 533
Eco31I GGTCTC 2 cut(s) 226, 559
Eco47I GGWCC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 571
Eco81I CCTNAGG 1 cut(s) 525
Eco88I CYCGRG 2 cut(s) 236, 317
EcoO109I RGGNCCY 2 cut(s) 340, 529
EcoT38I GRGCYC 2 cut(s) 317, 533
Esp3I CGTCTC 1 cut(s) 583
FaeI CATG 3 cut(s) 462, 501, 839
FaiI YATR 5 cut(s) 17, 460, 499, 837, 855
FaqI GGGAC 1 cut(s) 326
FatI CATG 3 cut(s) 458, 497, 835
FauI CCCGC 3 cut(s) 293, 683, 972
Fnu4HI GCNGC 6 cut(s) 73, 253, 278, 299, 545, 624
FokI GGATG 1 cut(s) 768
FriOI GRGCYC 2 cut(s) 317, 533
Fsp4HI GCNGC 6 cut(s) 73, 253, 278, 299, 545, 624
FspBI CTAG 2 cut(s) 735, 744
GlaI GCGC 2 cut(s) 302, 968
GluI GCNGC 6 cut(s) 73, 253, 278, 299, 545, 624
GsaI CCCAGC 1 cut(s) 522
HaeIII GGCC 4 cut(s) 455, 488, 523, 531
HapII CCGG 7 cut(s) 237, 344, 441, 551, 562, 644, 884
HgaI GACGC 2 cut(s) 565, 601
HhaI GCGC 2 cut(s) 303, 969
Hin1II CATG 3 cut(s) 462, 501, 839
Hin6I GCGC 2 cut(s) 301, 967
HinP1I GCGC 2 cut(s) 301, 967
HincII GTYRAC 1 cut(s) 265
HindII GTYRAC 1 cut(s) 265
HinfI GANTC 5 cut(s) 4, 329, 413, 449, 482
HpaI GTTAAC 1 cut(s) 265
HpaII CCGG 7 cut(s) 237, 344, 441, 551, 562, 644, 884
HphI GGTGA 5 cut(s) 125, 274, 491, 633, 873
Hpy166II GTNNAC 1 cut(s) 265
Hpy188I TCNGA 2 cut(s) 36, 231
Hpy188III TCNNGA 6 cut(s) 310, 326, 551, 1003, 1013, 1025
Hpy8I GTNNAC 1 cut(s) 265
Hpy99I CGWCG 2 cut(s) 53, 617
HpyAV CCTTC 4 cut(s) 17, 120, 153, 592
HpyCH4III ACNGT 3 cut(s) 763, 811, 982
HpyCH4IV ACGT 1 cut(s) 901
HpyCH4V TGCA 2 cut(s) 677, 842
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 609
HpyF3I CTNAG 3 cut(s) 223, 230, 525
HpySE526I ACGT 1 cut(s) 901
Hsp92II CATG 3 cut(s) 462, 501, 839
HspAI GCGC 2 cut(s) 301, 967
KflI GGGWCCC 1 cut(s) 340
Kpn2I TCCGGA 1 cut(s) 550
KspAI GTTAAC 1 cut(s) 265
Kzo9I GATC 8 cut(s) 240, 322, 392, 616, 800, 971, 985, 1015
LguI GCTCTTC 1 cut(s) 602
LmnI GCTCC 2 cut(s) 506, 934
Lsp1109I GCAGC 3 cut(s) 84, 285, 610
LweI GCATC 2 cut(s) 656, 664
MaeI CTAG 2 cut(s) 735, 744
MaeII ACGT 1 cut(s) 901
MaeIII GTNAC 3 cut(s) 479, 831, 879
MalI GATC 8 cut(s) 242, 324, 394, 618, 802, 973, 987, 1017
MboI GATC 8 cut(s) 240, 322, 392, 616, 800, 971, 985, 1015
MflI RGATCY 2 cut(s) 322, 985
MhlI GDGCHC 4 cut(s) 317, 533, 754, 931
MluCI AATT 3 cut(s) 424, 682, 706
MlyI GAGTC 4 cut(s) 323, 422, 458, 476
MmeI TCCRAC 1 cut(s) 956
MroI TCCGGA 1 cut(s) 550
MseI TTAA 2 cut(s) 264, 705
MspI CCGG 7 cut(s) 237, 344, 441, 551, 562, 644, 884
MspR9I CCNGG 4 cut(s) 237, 238, 344, 441
Mva1269I GAATGC 1 cut(s) 30
MvnI CGCG 1 cut(s) 967
MwoI GCNNNNNNNGC 2 cut(s) 370, 609
NciI CCSGG 4 cut(s) 237, 238, 344, 441
NdeII GATC 8 cut(s) 240, 322, 392, 616, 800, 971, 985, 1015
NlaIII CATG 3 cut(s) 462, 501, 839
NlaIV GGNNCC 5 cut(s) 341, 342, 456, 531, 987
NmeAIII GCCGAG 2 cut(s) 464, 695
NmuCI GTSAC 3 cut(s) 479, 831, 879
NspI RCATGY 1 cut(s) 839
PciI ACATGT 1 cut(s) 835
PciSI GCTCTTC 1 cut(s) 602
PctI GAATGC 1 cut(s) 30
PflFI GACNNNGTC 1 cut(s) 575
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 6 cut(s) 74, 254, 279, 300, 546, 625
PleI GAGTC 4 cut(s) 323, 421, 457, 476
PpsI GAGTC 4 cut(s) 323, 421, 457, 476
PpuMI RGGWCCY 1 cut(s) 340
PscI ACATGT 1 cut(s) 835
PsiI TTATAA 1 cut(s) 855
Psp5II RGGWCCY 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 518
PspN4I GGNNCC 5 cut(s) 341, 342, 456, 531, 987
PspOMI GGGCCC 1 cut(s) 529
PspPI GGNCC 6 cut(s) 340, 454, 487, 521, 529, 530
PspPPI RGGWCCY 1 cut(s) 340
PsrI GAACNNNNNNTAC 2 cut(s) 163, 195
PsuI RGATCY 2 cut(s) 322, 985
PsyI GACNNNGTC 1 cut(s) 575
SapI GCTCTTC 1 cut(s) 602
SaqAI TTAA 2 cut(s) 264, 705
SatI GCNGC 6 cut(s) 73, 253, 278, 299, 545, 624
Sau3AI GATC 8 cut(s) 240, 322, 392, 616, 800, 971, 985, 1015
Sau96I GGNCC 6 cut(s) 340, 454, 487, 521, 529, 530
SchI GAGTC 4 cut(s) 323, 422, 458, 476
ScrFI CCNGG 4 cut(s) 237, 238, 344, 441
SduI GDGCHC 4 cut(s) 317, 533, 754, 931
SfaNI GCATC 2 cut(s) 656, 664
SfcI CTRYAG 1 cut(s) 755
SgrAI CRCCGGYG 1 cut(s) 643
SinI GGWCC 1 cut(s) 340
SmaI CCCGGG 1 cut(s) 238
SmlI CTYRAG 3 cut(s) 772, 916, 946
SmoI CTYRAG 3 cut(s) 772, 916, 946
Sse9I AATT 3 cut(s) 424, 682, 706
SsiI CCGC 8 cut(s) 91, 252, 277, 286, 545, 690, 956, 965
SspI AATATT 1 cut(s) 380
SspMI CTAG 2 cut(s) 735, 744
StyD4I CCNGG 4 cut(s) 235, 236, 342, 439
TaaI ACNGT 3 cut(s) 763, 811, 982
TaiI ACGT 1 cut(s) 904
TaqI TCGA 3 cut(s) 327, 383, 615
TaqII GACCGA 2 cut(s) 456, 463
TasI AATT 3 cut(s) 424, 682, 706
TauI GCSGC 3 cut(s) 255, 280, 547
Tru1I TTAA 2 cut(s) 264, 705
Tru9I TTAA 2 cut(s) 264, 705
TscAI CASTG 5 cut(s) 262, 816, 882, 943, 985
TseFI GTSAC 3 cut(s) 479, 831, 879
TseI GCWGC 3 cut(s) 72, 298, 623
Tsp45I GTSAC 3 cut(s) 479, 831, 879
TspDTI ATGAA 1 cut(s) 177
TspGWI ACGGA 1 cut(s) 393
TspMI CCCGGG 1 cut(s) 236
TspRI CASTG 5 cut(s) 262, 816, 882, 943, 985
Tth111I GACNNNGTC 1 cut(s) 575
VpaK11BI GGWCC 1 cut(s) 340
XapI RAATTY 1 cut(s) 424
XceI RCATGY 1 cut(s) 839
XmaI CCCGGG 1 cut(s) 236
XspI CTAG 2 cut(s) 735, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.