Rroxscaffold_2G00138770

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001271
Physical Location & Seq
Reverse (-)
76957340 .. 76961559
4220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00138770.1

Sequence Viewer

Length: 756 bp
ATGGAAGTGAAATTGTTGAAGATCGGTTTTTTTATATATCTGGAGTATGTTCATCCTGGTTCTATTAACAAATATGTTCATGAACACTGTGGAGCCATGACAGAAGCTGTTGTTCGCAGTTTTACTCGCCATATAGTCTCTGGGTTGGCTTACTTGCACAGCACAAAAACAATACACAGGGACATCAAAGGGGCTAATTTGCTTGTGGATTCATGTGGGGTTGTCAAGCTAGCTGACTTTGGAATGGCCAAACATCTTTCAGGACACGTAGGGAATCTTTCCCTAAAGGGAAGTCCATACTGGATGGCTCCAGAGCTGATGCAATCTGAGATGCATAAAGATAACAGCTCTGATTTGGCTCTTGCTGTTGATATATGGAGTTTGGGTTGTACTATTATTGAAATGTTCACAGGAAAACCTCCATGGAGTGAGTATGAAGGGGCTGCAGCTATGTTTAAAGTTATGAAGGATACTCCACCATTTCCAGAAACATTGTCACCTGAGGGTAAGGACTTCCTTAGGTGTTGCTTCCAAAGAAATCCTGCTGAGAGGTCAACTGCTGCAATGCTATTAGAACATCGATTTATAAAGAGCTCCCAGCTGCAGGAAGTCCCATCTACGACCTATAATGCCTCTAATGGGATGAATTTCTTGGAAAGACCCCAGACTCCAATTGAACTTGAAAGAAGACTTGATAGTTCACTGATGATTCCCAGCATTGGCATGCAATTAACTACGGGAAAGGGGCCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

251

Amino Acids

27.98

Weight (kDa)

6.79

Isoelectric Point (pI)

41.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 11 - 196 1.3e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 11 - 192 2.6e-31 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 41 - 146 1.6e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AcoI YGGCCR 1 cut(s) 246
AcsI RAATTY 1 cut(s) 646
AfaI GTAC 1 cut(s) 391
AfiI CCNNNNNNNGG 3 cut(s) 604, 639, 719
AflIII ACRYGT 1 cut(s) 265
AgsI TTSAA 4 cut(s) 19, 401, 677, 683
AjnI CCWGG 1 cut(s) 55
AluBI AGCT 8 cut(s) 107, 229, 233, 316, 348, 449, 594, 601
AluI AGCT 8 cut(s) 107, 229, 233, 316, 348, 449, 594, 601
Alw21I GWGCWC 1 cut(s) 596
Alw26I GTCTC 1 cut(s) 142
AlwNI CAGNNNCTG 1 cut(s) 107
AoxI GGCC 2 cut(s) 246, 746
ApeKI GCWGC 4 cut(s) 443, 446, 560, 601
ApoI RAATTY 1 cut(s) 646
AspS9I GGNCC 1 cut(s) 746
AsuHPI GGTGA 1 cut(s) 489
AsuNHI GCTAGC 1 cut(s) 229
AxyI CCTNAGG 2 cut(s) 501, 518
BalI TGGCCA 1 cut(s) 248
BanII GRGCYC 1 cut(s) 596
BbsI GAAGAC 1 cut(s) 694
Bbv12I GWGCWC 1 cut(s) 596
BbvI GCAGC 4 cut(s) 430, 458, 547, 588
BccI CCATC 2 cut(s) 298, 622
BciT130I CCWGG 1 cut(s) 57
BciVI GTATCC 1 cut(s) 463
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 2 cut(s) 444, 602
BfuI GTATCC 1 cut(s) 463
BisI GCNGC 4 cut(s) 444, 447, 561, 602
BlsI GCNGC 4 cut(s) 445, 448, 562, 603
Bme1390I CCNGG 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 746
BmiI GGNNCC 3 cut(s) 94, 309, 747
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 2 cut(s) 309, 321
BmtI GCTAGC 1 cut(s) 233
BpiI GAAGAC 1 cut(s) 694
BpmI CTGGAG 2 cut(s) 62, 294
Bsa29I ATCGAT 1 cut(s) 580
BsaAI YACGTR 1 cut(s) 268
BsaJI CCNNGG 1 cut(s) 422
BsaXI ACNNNNNCTCC 2 cut(s) 370, 400
Bsc4I CCNNNNNNNGG 3 cut(s) 604, 639, 719
Bse1I ACTGG 1 cut(s) 305
Bse21I CCTNAGG 2 cut(s) 501, 518
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 1 cut(s) 57
BseCI ATCGAT 1 cut(s) 580
BseDI CCNNGG 1 cut(s) 422
BseGI GGATG 3 cut(s) 52, 309, 648
BseLI CCNNNNNNNGG 3 cut(s) 604, 639, 719
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 3 cut(s) 318, 492, 537
BseNI ACTGG 1 cut(s) 305
BseXI GCAGC 4 cut(s) 430, 458, 547, 588
BseYI CCCAGC 2 cut(s) 597, 713
BshFI GGCC 2 cut(s) 248, 748
BshVI ATCGAT 1 cut(s) 580
BsiHKAI GWGCWC 1 cut(s) 596
BslFI GGGAC 2 cut(s) 194, 596
BslI CCNNNNNNNGG 3 cut(s) 604, 639, 719
BsmAI GTCTC 1 cut(s) 142
BsmFI GGGAC 2 cut(s) 194, 596
BsnI GGCC 2 cut(s) 248, 748
Bsp1286I GDGCHC 1 cut(s) 596
Bsp143I GATC 1 cut(s) 21
Bsp19I CCATGG 1 cut(s) 422
BspANI GGCC 2 cut(s) 248, 748
BspCNI CTCAG 3 cut(s) 319, 493, 538
BspDI ATCGAT 1 cut(s) 580
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 3 cut(s) 94, 309, 747
BspMAI CTGCAG 2 cut(s) 448, 606
BspOI GCTAGC 1 cut(s) 233
BsrDI GCAATG 1 cut(s) 570
BsrI ACTGG 1 cut(s) 305
BssECI CCNNGG 1 cut(s) 422
BssMI GATC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 422
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 89
BstBAI YACGTR 1 cut(s) 268
BstC8I GCNNGC 2 cut(s) 231, 725
BstDEI CTNAG 4 cut(s) 327, 501, 518, 546
BstDSI CCRYGG 1 cut(s) 422
BstF5I GGATG 3 cut(s) 52, 309, 648
BstKTI GATC 1 cut(s) 24
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 1 cut(s) 21
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 1 cut(s) 727
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 2 cut(s) 444, 602
BstV1I GCAGC 4 cut(s) 430, 458, 547, 588
BstV2I GAAGAC 1 cut(s) 694
Bsu15I ATCGAT 1 cut(s) 580
Bsu36I CCTNAGG 2 cut(s) 501, 518
BsuI GTATCC 1 cut(s) 463
BsuRI GGCC 2 cut(s) 248, 748
BsuTUI ATCGAT 1 cut(s) 580
BtgI CCRYGG 1 cut(s) 422
BtsCI GGATG 3 cut(s) 52, 309, 648
BtsIMutI CAGTG 2 cut(s) 85, 701
Cac8I GCNNGC 2 cut(s) 231, 725
CaiI CAGNNNCTG 1 cut(s) 107
CciI TCATGA 1 cut(s) 79
Cfr13I GGNCC 1 cut(s) 746
ClaI ATCGAT 1 cut(s) 580
Csp6I GTAC 1 cut(s) 390
CviAII CATG 5 cut(s) 80, 97, 213, 423, 724
CviQI GTAC 1 cut(s) 390
DdeI CTNAG 4 cut(s) 327, 501, 518, 546
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
DraI TTTAAA 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 246
Ecl136II GAGCTC 1 cut(s) 594
Eco130I CCWWGG 1 cut(s) 422
Eco24I GRGCYC 1 cut(s) 596
Eco53kI GAGCTC 1 cut(s) 594
Eco81I CCTNAGG 2 cut(s) 501, 518
EcoICRI GAGCTC 1 cut(s) 594
EcoO109I RGGNCCY 1 cut(s) 746
EcoRII CCWGG 1 cut(s) 55
EcoT14I CCWWGG 1 cut(s) 422
EcoT22I ATGCAT 1 cut(s) 336
EcoT38I GRGCYC 1 cut(s) 596
ErhI CCWWGG 1 cut(s) 422
FaeI CATG 5 cut(s) 83, 100, 216, 426, 727
FaqI GGGAC 2 cut(s) 194, 596
FatI CATG 5 cut(s) 79, 96, 212, 422, 723
Fnu4HI GCNGC 4 cut(s) 444, 447, 561, 602
FokI GGATG 3 cut(s) 39, 316, 655
FriOI GRGCYC 1 cut(s) 596
Fsp4HI GCNGC 4 cut(s) 444, 447, 561, 602
FspBI CTAG 1 cut(s) 230
GluI GCNGC 4 cut(s) 444, 447, 561, 602
GsaI CCCAGC 2 cut(s) 601, 717
GsuI CTGGAG 2 cut(s) 62, 294
HaeIII GGCC 2 cut(s) 248, 748
Hin1II CATG 5 cut(s) 83, 100, 216, 426, 727
HincII GTYRAC 1 cut(s) 555
HindII GTYRAC 1 cut(s) 555
HinfI GANTC 4 cut(s) 209, 274, 667, 709
HphI GGTGA 1 cut(s) 489
Hpy166II GTNNAC 3 cut(s) 408, 555, 701
Hpy188I TCNGA 3 cut(s) 328, 352, 755
Hpy188III TCNNGA 5 cut(s) 41, 80, 261, 311, 485
Hpy8I GTNNAC 3 cut(s) 408, 555, 701
HpyAV CCTTC 2 cut(s) 431, 460
HpyCH4III ACNGT 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 7 cut(s) 157, 322, 334, 446, 563, 604, 727
HpyF3I CTNAG 4 cut(s) 327, 501, 518, 546
HpySE526I ACGT 1 cut(s) 267
Hsp92II CATG 5 cut(s) 83, 100, 216, 426, 727
Kzo9I GATC 1 cut(s) 21
LmnI GCTCC 3 cut(s) 92, 313, 599
Lsp1109I GCAGC 4 cut(s) 430, 458, 547, 588
LweI GCATC 2 cut(s) 309, 321
MaeI CTAG 1 cut(s) 230
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 1 cut(s) 495
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MboII GAAGA 2 cut(s) 31, 699
MfeI CAATTG 1 cut(s) 672
MhlI GDGCHC 1 cut(s) 596
MlsI TGGCCA 1 cut(s) 248
MluCI AATT 5 cut(s) 11, 196, 646, 672, 728
MluNI TGGCCA 1 cut(s) 248
MlyI GAGTC 1 cut(s) 661
MnlI CCTC 4 cut(s) 429, 496, 543, 643
Mox20I TGGCCA 1 cut(s) 248
Mph1103I ATGCAT 1 cut(s) 336
MscI TGGCCA 1 cut(s) 248
MseI TTAA 3 cut(s) 66, 456, 731
MslI CAYNNNNRTG 1 cut(s) 722
Msp20I TGGCCA 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 601
MspR9I CCNGG 1 cut(s) 57
MunI CAATTG 1 cut(s) 672
MvaI CCWGG 1 cut(s) 57
NcoI CCATGG 1 cut(s) 422
NdeII GATC 1 cut(s) 21
NheI GCTAGC 1 cut(s) 229
NlaIII CATG 5 cut(s) 83, 100, 216, 426, 727
NlaIV GGNNCC 3 cut(s) 94, 309, 747
NmuCI GTSAC 1 cut(s) 495
NsiI ATGCAT 1 cut(s) 336
NspI RCATGY 1 cut(s) 727
PaeI GCATGC 1 cut(s) 727
PagI TCATGA 1 cut(s) 79
PfeI GAWTC 3 cut(s) 209, 274, 709
PkrI GCNGC 4 cut(s) 445, 448, 562, 603
PleI GAGTC 1 cut(s) 661
PpsI GAGTC 1 cut(s) 661
Ppu21I YACGTR 1 cut(s) 268
PsiI TTATAA 1 cut(s) 587
Psp124BI GAGCTC 1 cut(s) 596
Psp6I CCWGG 1 cut(s) 55
PspFI CCCAGC 2 cut(s) 597, 713
PspGI CCWGG 1 cut(s) 55
PspN4I GGNNCC 3 cut(s) 94, 309, 747
PspPI GGNCC 1 cut(s) 746
PstI CTGCAG 2 cut(s) 448, 606
PstNI CAGNNNCTG 1 cut(s) 107
PvuII CAGCTG 1 cut(s) 601
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
RseI CAYNNNNRTG 1 cut(s) 722
SacI GAGCTC 1 cut(s) 596
SaqAI TTAA 3 cut(s) 66, 456, 731
SatI GCNGC 4 cut(s) 444, 447, 561, 602
Sau3AI GATC 1 cut(s) 21
Sau96I GGNCC 1 cut(s) 746
SchI GAGTC 1 cut(s) 661
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 1 cut(s) 596
SfaNI GCATC 2 cut(s) 309, 321
SfcI CTRYAG 2 cut(s) 444, 602
SmiMI CAYNNNNRTG 1 cut(s) 722
SphI GCATGC 1 cut(s) 727
Sse9I AATT 5 cut(s) 11, 196, 646, 672, 728
SspMI CTAG 1 cut(s) 230
SstI GAGCTC 1 cut(s) 596
StyD4I CCNGG 1 cut(s) 55
StyI CCWWGG 1 cut(s) 422
TaaI ACNGT 1 cut(s) 89
TaiI ACGT 1 cut(s) 270
TaqI TCGA 1 cut(s) 580
TasI AATT 5 cut(s) 11, 196, 646, 672, 728
TatI WGTACW 1 cut(s) 389
TfiI GAWTC 3 cut(s) 209, 274, 709
Tru1I TTAA 3 cut(s) 66, 456, 731
Tru9I TTAA 3 cut(s) 66, 456, 731
TscAI CASTG 2 cut(s) 92, 708
TseFI GTSAC 1 cut(s) 495
TseI GCWGC 4 cut(s) 443, 446, 560, 601
Tsp45I GTSAC 1 cut(s) 495
TspDTI ATGAA 7 cut(s) 41, 68, 96, 201, 450, 479, 659
TspRI CASTG 2 cut(s) 92, 708
XapI RAATTY 1 cut(s) 646
XceI RCATGY 1 cut(s) 727
XcmI CCANNNNNNNNNTGG 1 cut(s) 137
XspI CTAG 1 cut(s) 230
Zsp2I ATGCAT 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.