Rh6DG405100

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001271
Physical Location & Seq
Reverse (-)
59269075 .. 59274533
5459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG405100.1

Sequence Viewer

Length: 2094 bp
ATGCCTTCTTCTTCTTCTGCTCCATCTCAATCTCATCAAAAACGAACCTTTTTTACATCTTCATCTTCTACTCCTGCTTCCTCCTCCTCCTCCTCCTCCTCCTCCTCTGTCTCAGCTCTGAGACCGGGGATCAACAGAAGCGGCAGTGATGTTAACAGCCCACGGCGGCTCACGCGCCAGAGGAAGCTGCGCTACCTCACGAGCCCCGAGGATCTCGACTCTTACGGGGTCCCGGAGAAGTCTCGCTCATCGCCCACCTCGCCTGAATATTCGACCCGTAGATCACGCTCTCCGTGTGGCGAGTCAAATCCAAATTCACGCTTTCCCGGTGGCGAGTCGGCCCCATGTTCTCGGTCTTCGGTCGGTGACTCGGCCAGAAAGTCATGCTCCTCTTCTTTCGTTGCTGGGCCTCAAGGCCCACAACCCTTGCCGCTTCCGGAGAACAACCGGAGACCCGACTGCGTCTCTAAAGATAACAACTTCTTCAGCCCTGTGGTCGGCAGGAAGAGCGTCGATCAAGCTGCCACAAGCTCATCATCACCGGCGAAATCTTCCAAGAACCGAAGCCGAGATGTAAACAATTCCGGCGGGGGTGGGAGTGGGGGTTTTAATTTCCATTTGGGTTTGAATGTTCAATCTAGGACTGTTTTTAGCTCAAGTCATCATCATCCTGTTAGCCCACAAAGATCAAACACAGTGGATTTTTTGCCTTGTCAGTCACATGTTGCAGGCAACTTTTATAAACACAGGGGAATGGGAATGGGAATGGCAGTGTCACCGGAGGCTAACTTTGAACGTGAAACTTACAACTTGAGCAGGAGTGCTCCAACCAGTGTTCTCTCAAGTCCTGCAGTTAGCCCGCGCCGATCAACCACTGGGGATCCTTTTCCTTTTTTCATGACTCATCAAGATCAATATCAGGATGGTGCTACCTTTCTCTCGTTGTTGTCACCAAAGAAGCCTACACTCAGTCCCACTCATTCTCCACTCCATAGCCCTACACCTCGAAGTCCACGCGCATTGGGGCTGAATTCAAACTATGAGAGTAGTCTTGCCACTGCTCACCCGTTGCCTCTGCCTCCTGGAGCTGCAGTGCCAGCACAGCCGCCGTCTATCACTATGCATCAAAATAATGTAGCACCAAATGTAACCGTATCATCAATGAAAGGTCAATGGCAGAAGGGGAAACTTATCGGACGGGGTACCTTTGGTAGTGTTTATCTTGCAACCAACCGAGAAACTGGGGCCTTGTGTGCAATGAAGGAAGTTGATCTCATTCCTGATGATCCTAAATCTGCAGAAAGTATAAAGCAATTGGAGCAGGAAATTAAAGTCCTCCGTACACTAAAGCATCCAAATATTGTGCAGTACTATGGTAGTGAAGTGATTGATGATCATTTCTACATATATTTAGAATATGTTCACCCTGGATCAATAAATAAATATGTCCAAGACCATTTTGGAGCTATGACTGAATCTGTAATCCGCAATTTTACCCGCCATATCCTCCGTGGGTTGGCTTTTTTGCATGACACGAAGACAATTCACAGGGATATTAAAGGTGCAAATTTGCTTGTTGATGCATCAGGCGTTGTCAAACTTGCCGATTTTGGGATGGCAAAACATCTTAGTGCGCATTCATACAATCTTTCTTTGAAGGGCAGCCCATACTGGATGGCTCCTGAGGTCATAAAGGCTGTGATACAGAATGATGGTGATCCTAATCTTGCTTTGGCTGTTGATATATGGAGCTTGGGTTGTACCATCATCGAGATGTTCAATGGAAAACCGCCTTGGAGTGAATTTGAAGGGCCACAAGCTATGTTCAAGGTTCTGCACAGAACCCCGGATATACCAGAAAAATTATCTGCAGAAGGAAAGGAATTCCTCAGTTTGTGCTTCAAAAGGAACCCCGCAGAGAGGCCATCAGCTAAGAAGCTACTTGATCATCCTTTTGTACGAAATTCAAATGATCAAAATGTCTCATCTTATACTCGGGCGTTTTCTCTGGTAATGGATAAATTGAATAGCCCGACGGACCATATTAAGCAAAAACTTGGTCGAATGCTGGTCTATCCACGTACTTCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

697

Amino Acids

76.07

Weight (kDa)

9.6

Isoelectric Point (pI)

60.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 392 - 653 7.2e-68 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 395 - 648 4.8e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 741
Acc16I TGCGCA 1 cut(s) 1635
Acc65I GGTACC 1 cut(s) 1202
AccB1I GGYRCC 1 cut(s) 1202
AccII CGCG 3 cut(s) 175, 862, 1017
AccIII TCCGGA 1 cut(s) 436
AclWI GGATC 7 cut(s) 137, 219, 875, 888, 1280, 1438, 1712
AcoI YGGCCR 1 cut(s) 372
AcsI RAATTY 6 cut(s) 313, 1030, 1567, 1802, 1883, 1963
AcuI CTGAAG 1 cut(s) 469
AfaI GTAC 6 cut(s) 1204, 1342, 1370, 1762, 1959, 2083
AfiI CCNNNNNNNGG 4 cut(s) 497, 1516, 1611, 1920
AflIII ACRYGT 1 cut(s) 721
AjnI CCWGG 2 cut(s) 1081, 1426
AjuI GAANNNNNNNTTGG 2 cut(s) 1777, 1809
Alw21I GWGCWC 1 cut(s) 826
Alw26I GTCTC 6 cut(s) 115, 115, 246, 445, 469, 1987
AlwI GGATC 7 cut(s) 137, 219, 875, 888, 1280, 1438, 1712
Ama87I CYCGRG 2 cut(s) 206, 1995
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 7 cut(s) 339, 372, 407, 415, 1245, 1811, 1922
ApeKI GCWGC 4 cut(s) 187, 521, 1088, 1662
ApoI RAATTY 6 cut(s) 313, 1030, 1567, 1802, 1883, 1963
ArsI GACNNNNNNTTYG 2 cut(s) 2044, 2076
Asp700I GAANNNNTTC 1 cut(s) 1419
Asp718I GGTACC 1 cut(s) 1202
AspLEI GCGC 5 cut(s) 177, 192, 864, 1019, 1636
AspS9I GGNCC 7 cut(s) 229, 340, 407, 416, 1245, 1811, 2038
AsuC2I CCSGG 4 cut(s) 126, 233, 327, 1847
AsuHPI GGTGA 7 cut(s) 377, 531, 768, 942, 1055, 1415, 1727
AvaI CYCGRG 2 cut(s) 206, 1995
AvaII GGWCC 2 cut(s) 229, 2038
AxyI CCTNAGG 1 cut(s) 1683
BamHI GGATCC 1 cut(s) 880
BanI GGYRCC 1 cut(s) 1202
BanII GRGCYC 1 cut(s) 206
BarI GAAGNNNNNNTAC 2 cut(s) 176, 208
BauI CACGAG 1 cut(s) 199
BbsI GAAGAC 2 cut(s) 348, 1544
Bbv12I GWGCWC 1 cut(s) 826
BbvI GCAGC 4 cut(s) 174, 508, 1075, 1674
BccI CCATC 7 cut(s) 31, 917, 1609, 1669, 1706, 1772, 1933
BceAI ACGGC 2 cut(s) 179, 1093
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 2 cut(s) 1083, 1428
BclI TGATCA 3 cut(s) 1393, 1945, 1972
BcnI CCSGG 4 cut(s) 126, 233, 327, 1847
BcoDI GTCTC 6 cut(s) 115, 115, 246, 445, 469, 1987
BfaI CTAG 1 cut(s) 639
BfmI CTRYAG 4 cut(s) 849, 1089, 1296, 1869
BisI GCNGC 8 cut(s) 142, 167, 188, 431, 522, 1089, 1106, 1663
BlsI GCNGC 8 cut(s) 143, 168, 189, 432, 523, 1090, 1107, 1664
BmcAI AGTACT 1 cut(s) 1370
Bme1390I CCNGG 6 cut(s) 126, 233, 327, 1083, 1428, 1847
Bme18I GGWCC 2 cut(s) 229, 2038
BmeT110I CYCGRG 2 cut(s) 206, 1995
BmgT120I GGNCC 7 cut(s) 229, 340, 407, 416, 1245, 1811, 2038
BmiI GGNNCC 8 cut(s) 230, 231, 342, 882, 1204, 1246, 1680, 1910
BmrFI CCNGG 6 cut(s) 126, 233, 327, 1083, 1428, 1847
BmrI ACTGGG 2 cut(s) 885, 1251
BmsI GCATC 4 cut(s) 1132, 1360, 1570, 1592
BmuI ACTGGG 2 cut(s) 885, 1251
BpiI GAAGAC 2 cut(s) 348, 1544
BpmI CTGGAG 1 cut(s) 1104
BpuEI CTTGAG 4 cut(s) 396, 640, 826, 832
BpuMI CCSGG 4 cut(s) 126, 233, 327, 1847
BsaAI YACGTR 1 cut(s) 2081
BsaBI GATNNNNATC 3 cut(s) 915, 1716, 1722
BsaI GGTCTC 2 cut(s) 115, 445
BsaJI CCNNGG 7 cut(s) 125, 161, 207, 1426, 1510, 1793, 1845
BsaWI WCCGGW 3 cut(s) 436, 447, 778
BsaXI ACNNNNNCTCC 8 cut(s) 589, 619, 967, 997, 1077, 1107, 1741, 1771
Bsc4I CCNNNNNNNGG 4 cut(s) 497, 1516, 1611, 1920
Bse118I RCCGGY 1 cut(s) 541
Bse1I ACTGG 4 cut(s) 831, 880, 1246, 1676
Bse21I CCTNAGG 1 cut(s) 1683
Bse3DI GCAATG 1 cut(s) 1263
Bse8I GATNNNNATC 3 cut(s) 915, 1716, 1722
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 2 cut(s) 1083, 1428
BseDI CCNNGG 7 cut(s) 125, 161, 207, 1426, 1510, 1793, 1845
BseGI GGATG 6 cut(s) 667, 928, 1351, 1620, 1680, 1948
BseJI GATNNNNATC 3 cut(s) 915, 1716, 1722
BseLI CCNNNNNNNGG 4 cut(s) 497, 1516, 1611, 1920
BseMI GCAATG 1 cut(s) 1263
BseMII CTCAG 5 cut(s) 110, 126, 982, 1674, 1903
BseNI ACTGG 4 cut(s) 831, 880, 1246, 1676
BseRI GAGGAG 9 cut(s) 73, 76, 79, 82, 85, 88, 91, 94, 379
BseXI GCAGC 4 cut(s) 174, 508, 1075, 1674
BseYI CCCAGC 1 cut(s) 404
BsgI GTGCAG 2 cut(s) 1385, 1820
Bsh1236I CGCG 3 cut(s) 175, 862, 1017
Bsh1285I CGRYCG 1 cut(s) 363
BshFI GGCC 7 cut(s) 341, 374, 409, 417, 1247, 1813, 1924
BshNI GGYRCC 1 cut(s) 1202
BsiEI CGRYCG 1 cut(s) 363
BsiHKAI GWGCWC 1 cut(s) 826
BsiHKCI CYCGRG 2 cut(s) 206, 1995
BsiSI CCGG 9 cut(s) 125, 233, 327, 437, 448, 542, 585, 779, 1847
BslFI GGGAC 2 cut(s) 215, 957
BslI CCNNNNNNNGG 4 cut(s) 497, 1516, 1611, 1920
BsmAI GTCTC 6 cut(s) 115, 115, 246, 445, 469, 1987
BsmBI CGTCTC 1 cut(s) 469
BsmFI GGGAC 2 cut(s) 215, 957
BsmI GAATGC 2 cut(s) 1636, 2070
BsnI GGCC 7 cut(s) 341, 374, 409, 417, 1247, 1813, 1924
Bso31I GGTCTC 2 cut(s) 115, 445
BsoBI CYCGRG 2 cut(s) 206, 1995
Bsp1286I GDGCHC 2 cut(s) 206, 826
Bsp13I TCCGGA 1 cut(s) 436
BspANI GGCC 7 cut(s) 341, 374, 409, 417, 1247, 1813, 1924
BspCNI CTCAG 5 cut(s) 111, 125, 981, 1675, 1902
BspEI TCCGGA 1 cut(s) 436
BspFNI CGCG 3 cut(s) 175, 862, 1017
BspHI TCATGA 1 cut(s) 897
BspLI GGNNCC 8 cut(s) 230, 231, 342, 882, 1204, 1246, 1680, 1910
BspMAI CTGCAG 4 cut(s) 853, 1093, 1300, 1873
BspPI GGATC 7 cut(s) 137, 219, 875, 888, 1280, 1438, 1712
BspQI GCTCTTC 1 cut(s) 500
BspT107I GGYRCC 1 cut(s) 1202
BspTNI GGTCTC 2 cut(s) 115, 445
BsrDI GCAATG 1 cut(s) 1263
BsrFI RCCGGY 1 cut(s) 541
BsrI ACTGG 4 cut(s) 831, 880, 1246, 1676
BssAI RCCGGY 1 cut(s) 541
BssECI CCNNGG 7 cut(s) 125, 161, 207, 1426, 1510, 1793, 1845
BssSI CACGAG 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 1793
Bst2BI CACGAG 1 cut(s) 199
Bst2UI CCWGG 2 cut(s) 1083, 1428
Bst4CI ACNGT 3 cut(s) 646, 697, 1153
Bst6I CTCTTC 2 cut(s) 397, 500
BstBAI YACGTR 1 cut(s) 2081
BstC8I GCNNGC 3 cut(s) 730, 860, 1098
BstDEI CTNAG 7 cut(s) 112, 119, 968, 1628, 1683, 1889, 1932
BstDSI CCRYGG 2 cut(s) 161, 1510
BstF5I GGATG 6 cut(s) 667, 928, 1351, 1620, 1680, 1948
BstFNI CGCG 3 cut(s) 175, 862, 1017
BstHHI GCGC 5 cut(s) 177, 192, 864, 1019, 1636
BstMAI GTCTC 6 cut(s) 115, 115, 246, 445, 469, 1987
BstMCI CGRYCG 1 cut(s) 363
BstMWI GCNNNNNNNGC 7 cut(s) 172, 259, 507, 1097, 1102, 1253, 1318
BstNI CCWGG 2 cut(s) 1083, 1428
BstNSI RCATGY 1 cut(s) 725
BstSCI CCNGG 6 cut(s) 124, 231, 325, 1081, 1426, 1845
BstSFI CTRYAG 4 cut(s) 849, 1089, 1296, 1869
BstUI CGCG 3 cut(s) 175, 862, 1017
BstV1I GCAGC 4 cut(s) 174, 508, 1075, 1674
BstV2I GAAGAC 2 cut(s) 348, 1544
BstX2I RGATCY 2 cut(s) 211, 880
BstYI RGATCY 2 cut(s) 211, 880
Bsu36I CCTNAGG 1 cut(s) 1683
BsuRI GGCC 7 cut(s) 341, 374, 409, 417, 1247, 1813, 1924
BtgI CCRYGG 2 cut(s) 161, 1510
BtgZI GCGATG 1 cut(s) 234
BtsCI GGATG 6 cut(s) 667, 928, 1351, 1620, 1680, 1948
BtsI GCAGTG 4 cut(s) 151, 777, 1056, 1098
BtsIMutI CAGTG 7 cut(s) 151, 702, 777, 838, 873, 1056, 1098
Cac8I GCNNGC 3 cut(s) 730, 860, 1098
CciI TCATGA 1 cut(s) 897
CfoI GCGC 5 cut(s) 177, 192, 864, 1019, 1636
Cfr10I RCCGGY 1 cut(s) 541
Cfr13I GGNCC 7 cut(s) 229, 340, 407, 416, 1245, 1811, 2038
CseI GACGC 2 cut(s) 451, 499
Csp6I GTAC 6 cut(s) 1203, 1341, 1369, 1761, 1958, 2082
CspCI CAANNNNNGTGG 6 cut(s) 408, 443, 678, 713, 1002, 1037
CviAII CATG 5 cut(s) 345, 384, 722, 898, 1529
CviQI GTAC 6 cut(s) 1203, 1341, 1369, 1761, 1958, 2082
DdeI CTNAG 7 cut(s) 112, 119, 968, 1628, 1683, 1889, 1932
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 2 cut(s) 397, 500
EarI CTCTTC 2 cut(s) 397, 500
Eco130I CCWWGG 1 cut(s) 1793
Eco24I GRGCYC 1 cut(s) 206
Eco31I GGTCTC 2 cut(s) 115, 445
Eco47I GGWCC 2 cut(s) 229, 2038
Eco57I CTGAAG 1 cut(s) 469
Eco81I CCTNAGG 1 cut(s) 1683
Eco88I CYCGRG 2 cut(s) 206, 1995
EcoO109I RGGNCCY 2 cut(s) 229, 1245
EcoRI GAATTC 2 cut(s) 1030, 1883
EcoRII CCWGG 2 cut(s) 1081, 1426
EcoT14I CCWWGG 1 cut(s) 1793
EcoT22I ATGCAT 2 cut(s) 1125, 1585
EcoT38I GRGCYC 1 cut(s) 206
ErhI CCWWGG 1 cut(s) 1793
Esp3I CGTCTC 1 cut(s) 469
FaeI CATG 5 cut(s) 348, 387, 725, 901, 1532
FaqI GGGAC 2 cut(s) 215, 957
FatI CATG 5 cut(s) 344, 383, 721, 897, 1528
FauI CCCGC 4 cut(s) 581, 867, 1505, 1921
FbaI TGATCA 3 cut(s) 1393, 1945, 1972
Fnu4HI GCNGC 8 cut(s) 142, 167, 188, 431, 522, 1089, 1106, 1663
FokI GGATG 6 cut(s) 654, 935, 1338, 1627, 1687, 1935
FriOI GRGCYC 1 cut(s) 206
Fsp4HI GCNGC 8 cut(s) 142, 167, 188, 431, 522, 1089, 1106, 1663
FspAI RTGCGCAY 1 cut(s) 1635
FspBI CTAG 1 cut(s) 639
FspI TGCGCA 1 cut(s) 1635
GlaI GCGC 5 cut(s) 176, 191, 863, 1018, 1635
GluI GCNGC 8 cut(s) 142, 167, 188, 431, 522, 1089, 1106, 1663
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 1104
HaeIII GGCC 7 cut(s) 341, 374, 409, 417, 1247, 1813, 1924
HapII CCGG 9 cut(s) 125, 233, 327, 437, 448, 542, 585, 779, 1847
HgaI GACGC 2 cut(s) 451, 499
HhaI GCGC 5 cut(s) 177, 192, 864, 1019, 1636
Hin1II CATG 5 cut(s) 348, 387, 725, 901, 1532
Hin6I GCGC 5 cut(s) 175, 190, 862, 1017, 1634
HinP1I GCGC 5 cut(s) 175, 190, 862, 1017, 1634
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HinfI GANTC 6 cut(s) 218, 302, 335, 368, 901, 1475
HpaI GTTAAC 1 cut(s) 154
HpaII CCGG 9 cut(s) 125, 233, 327, 437, 448, 542, 585, 779, 1847
HphI GGTGA 7 cut(s) 377, 531, 768, 942, 1055, 1415, 1727
Hpy166II GTNNAC 5 cut(s) 154, 577, 1013, 1343, 1423
Hpy188I TCNGA 2 cut(s) 120, 1196
Hpy188III TCNNGA 9 cut(s) 199, 215, 437, 898, 908, 920, 1280, 1682, 1771
Hpy8I GTNNAC 5 cut(s) 154, 577, 1013, 1343, 1423
Hpy99I CGWCG 2 cut(s) 515, 2038
HpyAV CCTTC 6 cut(s) 15, 1174, 1255, 1651, 1802, 1868
HpyCH4III ACNGT 3 cut(s) 646, 697, 1153
HpyCH4IV ACGT 2 cut(s) 796, 2080
HpyF10VI GCNNNNNNNGC 7 cut(s) 172, 259, 507, 1097, 1102, 1253, 1318
HpyF3I CTNAG 7 cut(s) 112, 119, 968, 1628, 1683, 1889, 1932
HpySE526I ACGT 2 cut(s) 796, 2080
Hsp92II CATG 5 cut(s) 348, 387, 725, 901, 1532
HspAI GCGC 5 cut(s) 175, 190, 862, 1017, 1634
KflI GGGWCCC 1 cut(s) 229
Kpn2I TCCGGA 1 cut(s) 436
KpnI GGTACC 1 cut(s) 1206
Ksp22I TGATCA 3 cut(s) 1393, 1945, 1972
KspAI GTTAAC 1 cut(s) 154
LguI GCTCTTC 1 cut(s) 500
LmnI GCTCC 8 cut(s) 25, 392, 829, 1085, 1318, 1463, 1684, 1749
Lsp1109I GCAGC 4 cut(s) 174, 508, 1075, 1674
LweI GCATC 4 cut(s) 1132, 1360, 1570, 1592
MaeI CTAG 1 cut(s) 639
MaeII ACGT 2 cut(s) 796, 2080
MaeIII GTNAC 5 cut(s) 365, 717, 774, 948, 1147
MfeI CAATTG 1 cut(s) 1313
MflI RGATCY 2 cut(s) 211, 880
MhlI GDGCHC 2 cut(s) 206, 826
MlyI GAGTC 5 cut(s) 212, 311, 344, 362, 895
MmeI TCCRAC 1 cut(s) 851
Mph1103I ATGCAT 2 cut(s) 1125, 1585
MroI TCCGGA 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 1419
MseI TTAA 5 cut(s) 153, 609, 1329, 1557, 2046
MslI CAYNNNNRTG 2 cut(s) 1629, 1772
MspI CCGG 9 cut(s) 125, 233, 327, 437, 448, 542, 585, 779, 1847
MspR9I CCNGG 6 cut(s) 126, 233, 327, 1083, 1428, 1847
MunI CAATTG 1 cut(s) 1313
Mva1269I GAATGC 2 cut(s) 1636, 2070
MvaI CCWGG 2 cut(s) 1083, 1428
MvnI CGCG 3 cut(s) 175, 862, 1017
MwoI GCNNNNNNNGC 7 cut(s) 172, 259, 507, 1097, 1102, 1253, 1318
NciI CCSGG 4 cut(s) 126, 233, 327, 1847
NlaIII CATG 5 cut(s) 348, 387, 725, 901, 1532
NlaIV GGNNCC 8 cut(s) 230, 231, 342, 882, 1204, 1246, 1680, 1910
NmeAIII GCCGAG 2 cut(s) 350, 593
NmuCI GTSAC 4 cut(s) 365, 717, 774, 948
NsbI TGCGCA 1 cut(s) 1635
NsiI ATGCAT 2 cut(s) 1125, 1585
NspI RCATGY 1 cut(s) 725
PagI TCATGA 1 cut(s) 897
PciI ACATGT 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 500
PctI GAATGC 2 cut(s) 1636, 2070
PdmI GAANNNNTTC 1 cut(s) 1419
PfeI GAWTC 1 cut(s) 1475
PflFI GACNNNGTC 1 cut(s) 461
PfoI TCCNGGA 2 cut(s) 231, 1081
PkrI GCNGC 8 cut(s) 143, 168, 189, 432, 523, 1090, 1107, 1664
PleI GAGTC 5 cut(s) 212, 310, 343, 362, 895
PpsI GAGTC 5 cut(s) 212, 310, 343, 362, 895
Ppu21I YACGTR 1 cut(s) 2081
PpuMI RGGWCCY 1 cut(s) 229
PscI ACATGT 1 cut(s) 721
PsiI TTATAA 1 cut(s) 741
Psp5II RGGWCCY 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 1081, 1426
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 2 cut(s) 1081, 1426
PspN4I GGNNCC 8 cut(s) 230, 231, 342, 882, 1204, 1246, 1680, 1910
PspPI GGNCC 7 cut(s) 229, 340, 407, 416, 1245, 1811, 2038
PspPPI RGGWCCY 1 cut(s) 229
PsrI GAACNNNNNNTAC 2 cut(s) 37, 69
PstI CTGCAG 4 cut(s) 853, 1093, 1300, 1873
PsuI RGATCY 2 cut(s) 211, 880
PsyI GACNNNGTC 1 cut(s) 461
RsaI GTAC 6 cut(s) 1204, 1342, 1370, 1762, 1959, 2083
RsaNI GTAC 6 cut(s) 1203, 1341, 1369, 1761, 1958, 2082
RseI CAYNNNNRTG 2 cut(s) 1629, 1772
SapI GCTCTTC 1 cut(s) 500
SaqAI TTAA 5 cut(s) 153, 609, 1329, 1557, 2046
SatI GCNGC 8 cut(s) 142, 167, 188, 431, 522, 1089, 1106, 1663
Sau96I GGNCC 7 cut(s) 229, 340, 407, 416, 1245, 1811, 2038
ScaI AGTACT 1 cut(s) 1370
SchI GAGTC 5 cut(s) 212, 311, 344, 362, 895
ScrFI CCNGG 6 cut(s) 126, 233, 327, 1083, 1428, 1847
SduI GDGCHC 2 cut(s) 206, 826
SfaNI GCATC 4 cut(s) 1132, 1360, 1570, 1592
SfcI CTRYAG 4 cut(s) 849, 1089, 1296, 1869
SgrAI CRCCGGYG 1 cut(s) 541
SinI GGWCC 2 cut(s) 229, 2038
SmiMI CAYNNNNRTG 2 cut(s) 1629, 1772
SmlI CTYRAG 4 cut(s) 411, 655, 811, 841
SmoI CTYRAG 4 cut(s) 411, 655, 811, 841
SspI AATATT 2 cut(s) 269, 1360
SspMI CTAG 1 cut(s) 639
StyD4I CCNGG 6 cut(s) 124, 231, 325, 1081, 1426, 1845
StyI CCWWGG 1 cut(s) 1793
TaaI ACNGT 3 cut(s) 646, 697, 1153
TaiI ACGT 2 cut(s) 799, 2083
TaqI TCGA 6 cut(s) 216, 272, 513, 1006, 1770, 2062
TaqII GACCGA 2 cut(s) 342, 349
TatI WGTACW 1 cut(s) 1368
TauI GCSGC 4 cut(s) 144, 169, 433, 1108
TfiI GAWTC 1 cut(s) 1475
Tru1I TTAA 5 cut(s) 153, 609, 1329, 1557, 2046
Tru9I TTAA 5 cut(s) 153, 609, 1329, 1557, 2046
TscAI CASTG 7 cut(s) 151, 702, 777, 838, 880, 1063, 1098
TseFI GTSAC 4 cut(s) 365, 717, 774, 948
TseI GCWGC 4 cut(s) 187, 521, 1088, 1662
Tsp45I GTSAC 4 cut(s) 365, 717, 774, 948
TspDTI ATGAA 5 cut(s) 51, 886, 1178, 1274, 1629
TspGWI ACGGA 4 cut(s) 282, 1328, 1499, 2051
TspRI CASTG 7 cut(s) 151, 702, 777, 838, 880, 1063, 1098
Tth111I GACNNNGTC 1 cut(s) 461
VpaK11BI GGWCC 2 cut(s) 229, 2038
XapI RAATTY 6 cut(s) 313, 1030, 1567, 1802, 1883, 1963
XceI RCATGY 1 cut(s) 725
XcmI CCANNNNNNNNNTGG 2 cut(s) 1457, 1508
XmnI GAANNNNTTC 1 cut(s) 1419
XspI CTAG 1 cut(s) 639
ZrmI AGTACT 1 cut(s) 1370
Zsp2I ATGCAT 2 cut(s) 1125, 1585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.