Rw2G013600

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001271
Physical Location & Seq
Forward (+)
14711170 .. 14714664
3495 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013600.1

Sequence Viewer

Length: 1791 bp
ATGCTAGCCACAACCATGCGTTGGTTGCCTAGCTTTGCTTCCTCATCATCATCTGCTTCGTCCTCGTCGTCGTCGTCGCCGAAAAAGAAGGCCAGGGAGGACGGCGGGTGGATTTTCCGGTCGAGGAGGAAGGCCGCGCAGTCGAGGAAACTGCTCAATTTGGGGAGAGACAATGACGGTGGAGTTATAACCAAATGCTCCAGCGCGCCGGGGTCGGAATCCGCGCTGGCCAGTCCGCAGCCGCTTCCTTTGCCGGAATCGGCCATCCGCCGAGAGACGAGGTGTTCTCGCTGTAAGTCATGGGGACATGAGAAATGCTTCCCCTCCCCGGCTCCCAGTGTGTTTTCTTGGCGAGACACTCCAAAGAATAAGGTTCAAGTACGTGAGACAGTGTCGTCTAGGAGGAAGAATCATGATCGAAATCGTTTACAGATCCCTGAACATGACTTCAGGCTCGATGTTCCTGTTAGAAGTGCACCCACCAGTCCATGCGCAAGTCCTAGATTCAGCCCACGCAGACCAAGTGCTGCTGATTTGTGGGCTCCGTCCAGAAATCAAGTCTGGTCTGCACCGGAGATGACTACTAATGATATGACTCAACCTCCCCCTGCCTTATCAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTCCCACGGCTAGAAGTCCCCTTCGACCCCCACAAGCATCATCCCCATTGCATCCCCGGTTATCTGTTACCTCTGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGCCTCCCTTCACCTAGTGTACCATCACCACAACCTGCACTCACACCTAAGCCAGAGTCCCAGCCGATTAAAGGGCAATGGCAAAAGGGAAAGCTTATTGGTCGTGGCACCTTTGGCAGTGTTTATGTTGCTACCAATAGAGAAACTGGAGCTTTATGTGCAATGAAGGAAGTTGAACTACTTCCTGATGACCCGAAATCTGCAGAGTGTATAAAGCAGCTACAGCAGGAAATCAAAGTCCTCAGCCAGCTCAAGCATCCTAATATTGTGCAGTACTATGGAAGTGAAATTGTTGAAGATCGGTTTTTTATATATCTGGAATATGTTCATCCTGGTTCTATTAACAAATATGTTCATGAACACTGTGGAGCCATGACAGAAGCTGTTGTTCGCAGTTTTACTCGCCATATAGTCTCTGGGTTGGCTTACTTGCACAGCACAAAAACAATACACAGGGACATCAAAGGGGCTAATTTGCTTGTGGATTCATGTGGGGTTGTCAAGCTAGCTGACTTTGGAATGGCCAAACATCTTTCAGGACACGTAGGGAATCTTTCCCTAAAGGGAAGTCCATACTGGATGGCTCCAGAGCTGATGCAATCTGAGATGCATAAAGATAACAGCTCTGATTTGGCTCTTGCTGTTGATATATGGAGTTTGGGTTGTACTATTATTGAAATGTTCACAGGAAAACCTCCATGGAGTGAGTATGAAGGGGCTGCAGCTATGTTTAAAGTTATGAAGGATACTCCACCTTTTCCAGAAACATTGTCACCTGAGGGTAAGGACTTCCTTAGGTGCTGCTTCCAAAGAAATCCTGCTGAGAGGTCAACTGCTGCAATGCTATTAGAACATCGATTTATAAAAAACTCCCAGCTGCAGGAAGTCCCATCTACGACCTATAATGCCTCTAACGGGATGAATTTCTTGGAAAGACCCCAGACTCCAATTGAACTTGAAAGAAGACTTGATAGTTCACTGATGATTCCCAGCATTGGCATGCAATTAACTACGGGAAAGGGGCCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

596

Amino Acids

65.75

Weight (kDa)

9.29

Isoelectric Point (pI)

64.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 280 - 541 1.7e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 283 - 537 2.6e-44 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 386 - 491 9.7e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 188, 1622
AasI GACNNNNNNGTC 1 cut(s) 394
Acc16I TGCGCA 1 cut(s) 493
Acc36I ACCTGC 1 cut(s) 802
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 21
AccII CGCG 3 cut(s) 137, 206, 224
AciI CCGC 6 cut(s) 105, 135, 222, 236, 242, 268
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 3 cut(s) 228, 261, 1281
AcsI RAATTY 1 cut(s) 1681
AcuI CTGAAG 2 cut(s) 433, 747
AdeI CACNNNGTG 1 cut(s) 776
AfaI GTAC 4 cut(s) 381, 780, 1034, 1426
AfiI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1639, 1674, 1754
AflIII ACRYGT 1 cut(s) 1300
AgsI TTSAA 6 cut(s) 377, 935, 1055, 1436, 1712, 1718
AjnI CCWGG 3 cut(s) 92, 751, 1090
Alw21I GWGCWC 1 cut(s) 478
Alw26I GTCTC 5 cut(s) 162, 269, 348, 380, 1177
Alw44I GTGCAC 1 cut(s) 474
AlwI GGATC 1 cut(s) 427
AlwNI CAGNNNCTG 1 cut(s) 1142
AoxI GGCC 6 cut(s) 90, 132, 228, 261, 1281, 1781
ApaLI GTGCAC 1 cut(s) 474
ApeKI GCWGC 8 cut(s) 238, 527, 976, 1478, 1481, 1560, 1595, 1636
ApoI RAATTY 1 cut(s) 1681
Asp700I GAANNNNTTC 3 cut(s) 317, 939, 1083
AspLEI GCGC 5 cut(s) 139, 206, 208, 226, 494
AspS9I GGNCC 1 cut(s) 1781
AsuC2I CCSGG 3 cut(s) 210, 329, 709
AsuHPI GGTGA 3 cut(s) 762, 777, 1524
AsuNHI GCTAGC 2 cut(s) 4, 1264
AxyI CCTNAGG 2 cut(s) 1536, 1553
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 2 cut(s) 230, 1283
BanI GGYRCC 1 cut(s) 866
BanII GRGCYC 1 cut(s) 544
BarI GAAGNNNNNNTAC 2 cut(s) 921, 953
BbsI GAAGAC 1 cut(s) 1729
Bbv12I GWGCWC 1 cut(s) 478
BbvCI CCTCAGC 1 cut(s) 1001
BbvI GCAGC 8 cut(s) 250, 514, 988, 1465, 1493, 1547, 1582, 1623
BccI CCATC 5 cut(s) 272, 741, 790, 1333, 1657
BceAI ACGGC 2 cut(s) 118, 675
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 3 cut(s) 94, 753, 1092
BciVI GTATCC 1 cut(s) 1498
BcnI CCSGG 3 cut(s) 210, 329, 709
BcoDI GTCTC 5 cut(s) 162, 269, 348, 380, 1177
BfaI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1265
BfmI CTRYAG 4 cut(s) 960, 980, 1479, 1637
BfuAI ACCTGC 1 cut(s) 802
BfuI GTATCC 1 cut(s) 1498
BmcAI AGTACT 1 cut(s) 1034
Bme1390I CCNGG 6 cut(s) 94, 210, 329, 709, 753, 1092
BmgT120I GGNCC 1 cut(s) 1781
BmiI GGNNCC 6 cut(s) 333, 543, 868, 1129, 1344, 1782
BmrFI CCNGG 6 cut(s) 94, 210, 329, 709, 753, 1092
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 5 cut(s) 698, 712, 1024, 1344, 1356
BmtI GCTAGC 2 cut(s) 8, 1268
BmuI ACTGGG 1 cut(s) 330
BpiI GAAGAC 1 cut(s) 1729
BplI GAGNNNNNCTC 4 cut(s) 271, 303, 747, 779
BpmI CTGGAG 4 cut(s) 184, 774, 927, 1329
Bpu10I CCTNAGC 2 cut(s) 807, 1001
BpuEI CTTGAG 1 cut(s) 995
BpuMI CCSGG 3 cut(s) 210, 329, 709
Bsa29I ATCGAT 1 cut(s) 1615
BsaAI YACGTR 2 cut(s) 383, 1303
BsaBI GATNNNNATC 1 cut(s) 420
BsaJI CCNNGG 6 cut(s) 93, 209, 327, 657, 707, 1457
BsaWI WCCGGW 2 cut(s) 117, 571
BsaXI ACNNNNNCTCC 4 cut(s) 586, 616, 1405, 1435
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1639, 1674, 1754
Bse1I ACTGG 5 cut(s) 231, 336, 483, 910, 1340
Bse21I CCTNAGG 2 cut(s) 1536, 1553
Bse3DI GCAATG 5 cut(s) 698, 737, 842, 927, 1605
Bse8I GATNNNNATC 1 cut(s) 420
BseBI CCWGG 3 cut(s) 94, 753, 1092
BseCI ATCGAT 1 cut(s) 1615
BseDI CCNNGG 6 cut(s) 93, 209, 327, 657, 707, 1457
BseGI GGATG 8 cut(s) 264, 692, 703, 733, 1015, 1087, 1344, 1683
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1639, 1674, 1754
BseMI GCAATG 5 cut(s) 698, 737, 842, 927, 1605
BseMII CTCAG 4 cut(s) 1015, 1353, 1527, 1572
BseNI ACTGG 5 cut(s) 231, 336, 483, 910, 1340
BsePI GCGCGC 1 cut(s) 204
BseRI GAGGAG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 8 cut(s) 250, 514, 988, 1465, 1493, 1547, 1582, 1623
BseYI CCCAGC 3 cut(s) 819, 1632, 1748
BsgI GTGCAG 3 cut(s) 552, 780, 1049
Bsh1236I CGCG 3 cut(s) 137, 206, 224
Bsh1285I CGRYCG 1 cut(s) 122
BshFI GGCC 6 cut(s) 92, 134, 230, 263, 1283, 1783
BshNI GGYRCC 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 1615
BsiEI CGRYCG 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 478
BsiSI CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
BslFI GGGAC 6 cut(s) 318, 639, 654, 802, 1229, 1631
BslI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1639, 1674, 1754
BsmAI GTCTC 5 cut(s) 162, 269, 348, 380, 1177
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 6 cut(s) 318, 639, 654, 802, 1229, 1631
BsnI GGCC 6 cut(s) 92, 134, 230, 263, 1283, 1783
Bsp1286I GDGCHC 2 cut(s) 478, 544
Bsp143I GATC 3 cut(s) 415, 432, 1057
Bsp19I CCATGG 1 cut(s) 1457
BspACI CCGC 6 cut(s) 105, 135, 222, 236, 242, 268
BspANI GGCC 6 cut(s) 92, 134, 230, 263, 1283, 1783
BspCNI CTCAG 4 cut(s) 1014, 1354, 1528, 1573
BspDI ATCGAT 1 cut(s) 1615
BspFNI CGCG 3 cut(s) 137, 206, 224
BspHI TCATGA 2 cut(s) 412, 1114
BspLI GGNNCC 6 cut(s) 333, 543, 868, 1129, 1344, 1782
BspMAI CTGCAG 3 cut(s) 964, 1483, 1641
BspMI ACCTGC 1 cut(s) 802
BspOI GCTAGC 2 cut(s) 8, 1268
BspPI GGATC 1 cut(s) 427
BspT107I GGYRCC 1 cut(s) 866
BsrDI GCAATG 5 cut(s) 698, 737, 842, 927, 1605
BsrI ACTGG 5 cut(s) 231, 336, 483, 910, 1340
BssECI CCNNGG 6 cut(s) 93, 209, 327, 657, 707, 1457
BssHII GCGCGC 1 cut(s) 204
BssMI GATC 3 cut(s) 415, 432, 1057
BssT1I CCWWGG 1 cut(s) 1457
Bst2UI CCWGG 3 cut(s) 94, 753, 1092
Bst4CI ACNGT 4 cut(s) 179, 391, 653, 1124
BstBAI YACGTR 2 cut(s) 383, 1303
BstC8I GCNNGC 7 cut(s) 6, 206, 228, 760, 1007, 1266, 1760
BstDEI CTNAG 6 cut(s) 807, 1001, 1362, 1536, 1553, 1581
BstDSI CCRYGG 2 cut(s) 657, 1457
BstF5I GGATG 8 cut(s) 264, 692, 703, 733, 1015, 1087, 1344, 1683
BstFNI CGCG 3 cut(s) 137, 206, 224
BstHHI GCGC 5 cut(s) 139, 206, 208, 226, 494
BstKTI GATC 3 cut(s) 418, 435, 1060
BstMAI GTCTC 5 cut(s) 162, 269, 348, 380, 1177
BstMBI GATC 3 cut(s) 415, 432, 1057
BstMCI CGRYCG 1 cut(s) 122
BstMWI GCNNNNNNNGC 5 cut(s) 25, 250, 873, 917, 982
BstNI CCWGG 3 cut(s) 94, 753, 1092
BstNSI RCATGY 1 cut(s) 1762
BstSCI CCNGG 6 cut(s) 92, 208, 327, 707, 751, 1090
BstSFI CTRYAG 4 cut(s) 960, 980, 1479, 1637
BstSLI GKGCMC 1 cut(s) 478
BstUI CGCG 3 cut(s) 137, 206, 224
BstV1I GCAGC 8 cut(s) 250, 514, 988, 1465, 1493, 1547, 1582, 1623
BstV2I GAAGAC 1 cut(s) 1729
BstX2I RGATCY 1 cut(s) 432
BstYI RGATCY 1 cut(s) 432
Bsu15I ATCGAT 1 cut(s) 1615
Bsu36I CCTNAGG 2 cut(s) 1536, 1553
BsuI GTATCC 1 cut(s) 1498
BsuRI GGCC 6 cut(s) 92, 134, 230, 263, 1283, 1783
BsuTUI ATCGAT 1 cut(s) 1615
BtgI CCRYGG 2 cut(s) 657, 1457
BtsCI GGATG 8 cut(s) 264, 692, 703, 733, 1015, 1087, 1344, 1683
BtsI GCAGTG 1 cut(s) 883
BtsIMutI CAGTG 5 cut(s) 343, 396, 883, 1120, 1736
BveI ACCTGC 1 cut(s) 802
Cac8I GCNNGC 7 cut(s) 6, 206, 228, 760, 1007, 1266, 1760
CaiI CAGNNNCTG 1 cut(s) 1142
CciI TCATGA 2 cut(s) 412, 1114
CfoI GCGC 5 cut(s) 139, 206, 208, 226, 494
Cfr13I GGNCC 1 cut(s) 1781
ClaI ATCGAT 1 cut(s) 1615
Csp6I GTAC 4 cut(s) 380, 779, 1033, 1425
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviQI GTAC 4 cut(s) 380, 779, 1033, 1425
DdeI CTNAG 6 cut(s) 807, 1001, 1362, 1536, 1553, 1581
DpnI GATC 3 cut(s) 417, 434, 1059
DpnII GATC 3 cut(s) 415, 432, 1057
DraI TTTAAA 1 cut(s) 1492
DraIII CACNNNGTG 1 cut(s) 776
DrdI GACNNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 3 cut(s) 228, 261, 1281
EciI GGCGGA 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 1457
Eco24I GRGCYC 1 cut(s) 544
Eco57I CTGAAG 2 cut(s) 433, 747
Eco81I CCTNAGG 2 cut(s) 1536, 1553
EcoO109I RGGNCCY 1 cut(s) 1781
EcoRII CCWGG 3 cut(s) 92, 751, 1090
EcoT14I CCWWGG 1 cut(s) 1457
EcoT22I ATGCAT 1 cut(s) 1371
EcoT38I GRGCYC 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 1457
Esp3I CGTCTC 1 cut(s) 269
FalI AAGNNNNNCTT 2 cut(s) 751, 783
FaqI GGGAC 6 cut(s) 318, 639, 654, 802, 1229, 1631
FauI CCCGC 1 cut(s) 98
FokI GGATG 8 cut(s) 251, 679, 690, 720, 1002, 1074, 1351, 1690
FriOI GRGCYC 1 cut(s) 544
FspBI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1265
FspI TGCGCA 1 cut(s) 493
GlaI GCGC 5 cut(s) 138, 205, 207, 225, 493
GsaI CCCAGC 3 cut(s) 823, 1636, 1752
GsuI CTGGAG 4 cut(s) 184, 774, 927, 1329
HaeIII GGCC 6 cut(s) 92, 134, 230, 263, 1283, 1783
HapII CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
HhaI GCGC 5 cut(s) 139, 206, 208, 226, 494
Hin6I GCGC 5 cut(s) 137, 204, 206, 224, 492
HinP1I GCGC 5 cut(s) 137, 204, 206, 224, 492
HincII GTYRAC 1 cut(s) 1590
HindII GTYRAC 1 cut(s) 1590
HindIII AAGCTT 1 cut(s) 851
HpaII CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
HphI GGTGA 3 cut(s) 762, 777, 1524
Hpy166II GTNNAC 6 cut(s) 428, 476, 779, 1443, 1590, 1736
Hpy188I TCNGA 6 cut(s) 217, 619, 727, 1363, 1387, 1790
Hpy188III TCNNGA 8 cut(s) 413, 549, 944, 1076, 1115, 1296, 1346, 1520
Hpy8I GTNNAC 6 cut(s) 428, 476, 779, 1443, 1590, 1736
Hpy99I CGWCG 4 cut(s) 70, 73, 76, 79
HpyAV CCTTC 7 cut(s) 82, 124, 683, 777, 919, 1466, 1495
HpyCH4III ACNGT 4 cut(s) 179, 391, 653, 1124
HpyCH4IV ACGT 2 cut(s) 382, 1302
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 250, 873, 917, 982
HpyF3I CTNAG 6 cut(s) 807, 1001, 1362, 1536, 1553, 1581
HpySE526I ACGT 2 cut(s) 382, 1302
HspAI GCGC 5 cut(s) 137, 204, 206, 224, 492
Kzo9I GATC 3 cut(s) 415, 432, 1057
LmnI GCTCC 7 cut(s) 203, 337, 547, 755, 908, 1127, 1348
Lsp1109I GCAGC 8 cut(s) 250, 514, 988, 1465, 1493, 1547, 1582, 1623
LweI GCATC 5 cut(s) 698, 712, 1024, 1344, 1356
MaeI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1265
MaeII ACGT 2 cut(s) 382, 1302
MaeIII GTNAC 2 cut(s) 718, 1530
MalI GATC 3 cut(s) 417, 434, 1059
MboI GATC 3 cut(s) 415, 432, 1057
MboII GAAGA 3 cut(s) 418, 1067, 1734
MfeI CAATTG 1 cut(s) 1707
MflI RGATCY 1 cut(s) 432
MhlI GDGCHC 2 cut(s) 478, 544
MlsI TGGCCA 2 cut(s) 230, 1283
MluCI AATT 6 cut(s) 157, 1047, 1231, 1681, 1707, 1763
MluNI TGGCCA 2 cut(s) 230, 1283
MlyI GAGTC 3 cut(s) 589, 824, 1696
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 2 cut(s) 230, 1283
Mph1103I ATGCAT 1 cut(s) 1371
MroXI GAANNNNTTC 3 cut(s) 317, 939, 1083
MscI TGGCCA 2 cut(s) 230, 1283
MseI TTAA 4 cut(s) 828, 1101, 1491, 1766
MslI CAYNNNNRTG 2 cut(s) 14, 1757
Msp20I TGGCCA 2 cut(s) 230, 1283
MspA1I CMGCKG 1 cut(s) 1636
MspI CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
MspR9I CCNGG 6 cut(s) 94, 210, 329, 709, 753, 1092
MunI CAATTG 1 cut(s) 1707
MvaI CCWGG 3 cut(s) 94, 753, 1092
MvnI CGCG 3 cut(s) 137, 206, 224
MwoI GCNNNNNNNGC 5 cut(s) 25, 250, 873, 917, 982
NciI CCSGG 3 cut(s) 210, 329, 709
NcoI CCATGG 1 cut(s) 1457
NdeII GATC 3 cut(s) 415, 432, 1057
NheI GCTAGC 2 cut(s) 4, 1264
NlaIV GGNNCC 6 cut(s) 333, 543, 868, 1129, 1344, 1782
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 1530
NsbI TGCGCA 1 cut(s) 493
NsiI ATGCAT 1 cut(s) 1371
NspI RCATGY 1 cut(s) 1762
PaeI GCATGC 1 cut(s) 1762
PagI TCATGA 2 cut(s) 412, 1114
PauI GCGCGC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 3 cut(s) 65, 71, 77
PdmI GAANNNNTTC 3 cut(s) 317, 939, 1083
PfeI GAWTC 7 cut(s) 218, 257, 409, 504, 1244, 1309, 1744
PflFI GACNNNGTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 751
PleI GAGTC 3 cut(s) 589, 823, 1696
PpsI GAGTC 3 cut(s) 589, 823, 1696
Ppu21I YACGTR 2 cut(s) 383, 1303
PsiI TTATAA 2 cut(s) 188, 1622
Psp6I CCWGG 3 cut(s) 92, 751, 1090
PspFI CCCAGC 3 cut(s) 819, 1632, 1748
PspGI CCWGG 3 cut(s) 92, 751, 1090
PspN4I GGNNCC 6 cut(s) 333, 543, 868, 1129, 1344, 1782
PspPI GGNCC 1 cut(s) 1781
PsrI GAACNNNNNNTAC 2 cut(s) 622, 654
PstI CTGCAG 3 cut(s) 964, 1483, 1641
PstNI CAGNNNCTG 1 cut(s) 1142
PsuI RGATCY 1 cut(s) 432
PsyI GACNNNGTC 1 cut(s) 391
PteI GCGCGC 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 1636
RsaI GTAC 4 cut(s) 381, 780, 1034, 1426
RsaNI GTAC 4 cut(s) 380, 779, 1033, 1425
RseI CAYNNNNRTG 2 cut(s) 14, 1757
SaqAI TTAA 4 cut(s) 828, 1101, 1491, 1766
Sau3AI GATC 3 cut(s) 415, 432, 1057
Sau96I GGNCC 1 cut(s) 1781
ScaI AGTACT 1 cut(s) 1034
SchI GAGTC 3 cut(s) 589, 824, 1696
ScrFI CCNGG 6 cut(s) 94, 210, 329, 709, 753, 1092
SduI GDGCHC 2 cut(s) 478, 544
SfaNI GCATC 5 cut(s) 698, 712, 1024, 1344, 1356
SfcI CTRYAG 4 cut(s) 960, 980, 1479, 1637
SmiMI CAYNNNNRTG 2 cut(s) 14, 1757
SmlI CTYRAG 1 cut(s) 1010
SmoI CTYRAG 1 cut(s) 1010
SphI GCATGC 1 cut(s) 1762
Sse9I AATT 6 cut(s) 157, 1047, 1231, 1681, 1707, 1763
SsiI CCGC 6 cut(s) 105, 135, 222, 236, 242, 268
SspI AATATT 1 cut(s) 1024
SspMI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1265
StyD4I CCNGG 6 cut(s) 92, 208, 327, 707, 751, 1090
StyI CCWWGG 1 cut(s) 1457
TaaI ACNGT 4 cut(s) 179, 391, 653, 1124
TaiI ACGT 2 cut(s) 385, 1305
TaqI TCGA 6 cut(s) 122, 143, 418, 456, 676, 1615
TasI AATT 6 cut(s) 157, 1047, 1231, 1681, 1707, 1763
TatI WGTACW 2 cut(s) 1032, 1424
TauI GCSGC 2 cut(s) 137, 244
TfiI GAWTC 7 cut(s) 218, 257, 409, 504, 1244, 1309, 1744
Tru1I TTAA 4 cut(s) 828, 1101, 1491, 1766
Tru9I TTAA 4 cut(s) 828, 1101, 1491, 1766
TscAI CASTG 5 cut(s) 343, 396, 883, 1127, 1743
TseFI GTSAC 1 cut(s) 1530
TseI GCWGC 8 cut(s) 238, 527, 976, 1478, 1481, 1560, 1595, 1636
Tsp45I GTSAC 1 cut(s) 1530
TspDTI ATGAA 9 cut(s) 648, 938, 1076, 1103, 1131, 1236, 1485, 1514, 1694
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 5 cut(s) 343, 396, 883, 1127, 1743
Tth111I GACNNNGTC 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 21
VneI GTGCAC 1 cut(s) 474
XapI RAATTY 1 cut(s) 1681
XceI RCATGY 1 cut(s) 1762
XcmI CCANNNNNNNNNTGG 1 cut(s) 1172
XmnI GAANNNNTTC 3 cut(s) 317, 939, 1083
XspI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1265
ZrmI AGTACT 1 cut(s) 1034
Zsp2I ATGCAT 1 cut(s) 1371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.