RchiOBHm_Chr2g0104811

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001271
Physical Location & Seq
Forward (+)
16108766 .. 16112617
3852 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47905

Sequence Viewer

Length: 1770 bp
ATGCTAGCCACAACCATGCGTTGGTTGCCTAGCTTTGCTTCCTCATCATCATCTGCTTCGTCTTCGTCGCCAAAAAAGAAGGCCAGGGAGGACGGCGGGTGGATTTTCCGGTCGAGGAGGAAGGCCGCGCAGTCGAGGAAACTGCTCAATTTGGGGAGAGACAATGACGGTGGAGCTATAACCAAATGCTCCAGCGCGCCGGGGTCGGAATCCGCGCTGGCCAGTCCGCAGCCGCTTCCTTTGCCGGAATCGGCCATCCGCCGAGAGACGAGGTGTTCTCGCTGTAAGTCATGGGGACATGAGAACTGCTTCCTCTCCCCGGCTCCCAGTGTGTTTTCTTGGCGAGACACTCCAAAGAATAACGTTCGAGTACGTGAGACAGTGTCGTCTAGGAGGAAGAATCAAGATCGAAATCGTTTACAGATCCCTGAACATGACTTCAGGCTCGATGTTCCTGTTAGAAGTGCACCCACTAGTCCATGCGCAAGTCCTAGATTCAGCCCACGCAGACCAAGTGCTGCTGATTTGTGGGCTCCGTCCAGAAATCAAGTCTGGTCTGCACCGGAGATGACTACTAATGATATGACTCAACCTCCCCCTGCCTCATCAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTCCCACGGCTAGAAGTCCCCTTCGACCCCCACAAGCATCATCCCCATTGCATCCCCGGCTAGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGCCTTCCTTCACCTAGTGTACCATCACCACAACCTCCACTCACACCTAAGCCAGAGCCCCAGCCGATTAAAGGGCAATGGCAAAAGGGAAAGCTTATTGGTCGTGGCACCTTTGGCAGTGTTTATGTTGCTACCAATAGAGAAACTGGAGCTTTATGTGCAATGAAGGAAGTTGAACTACTTCCTGATGACCCGAAATCAGCAGAGTGTATAAAGCAGCTACAGCAGGAAATCAAAGTCCTCAGCCAGCTCAAGCATCCTAATATTGTGCAGTACTATGGAAGTGAAATTGTTGAAGATCGGTTTTTTATATATCTGGAGTATGTTCATCCTGGTTCTATTAACAAATATGTTCATGAACACTGTGGAGCCATGACAGAAGCTGTTGTTCGCAGTTTTACTCGCCATATAGTCTCTGGGTTGGCTTACTTGCACAGCACAAAAACAATACACAGGGACATCAAAGGGGCTAATTTGCTTGTGGATTCATGTGGGGTTGTCAAGCTAGCTGACTTTGGAATGGCCAAACATCTTTCAGGACATGTAGGGAATCTTTCCCTAAAGGGAAGTCCATACTGGATGGCTCCAGAGCTGATGCAATCTGAGATGCATAAAGATAACAGCTCTGATTTGGCTCTTGCTGTTGATATATGGAGTTTGGGTTGTACTATAATTGAAATGTTCACAGGAAAACCTCCATGGAGTGAGTATGAAGGGGCTGCAGCTATGTTTAAAGTTATGAAGGATACTCCACCATTTCCAGAAACATTGTCACCTGAGGGTAAGGACTTCCTTAGGTGCTGCTTCCAAAGAAATCCTGCTGAGAGGTCAACTGCTGCAATGCTATTAGAACATCGATTTATAAAAAACTCCCAGCTGCAGGAAGTCCCATCTACGACCTATAATGCCTCTAATGGGATGAATTTCTTGGAAAGACCCCAGACTCCAATTGAACTTGAAAGAAGACTTGATAGTTCATTGATGATTCCCAGCATTGGCATGCAATTAACTACGGGAAAGGCGGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

589

Amino Acids

65.09

Weight (kDa)

9.25

Isoelectric Point (pI)

63.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 273 - 534 1.7e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 276 - 530 2.6e-44 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 379 - 484 9.6e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1601
AasI GACNNNNNNGTC 1 cut(s) 385
Acc16I TGCGCA 1 cut(s) 484
AccB1I GGYRCC 1 cut(s) 845
AccB7I CCANNNNNTGG 1 cut(s) 21
AccII CGCG 3 cut(s) 128, 197, 215
AciI CCGC 7 cut(s) 96, 126, 213, 227, 233, 259, 1760
AclI AACGTT 1 cut(s) 363
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 3 cut(s) 219, 252, 1260
AcsI RAATTY 1 cut(s) 1660
AcuI CTGAAG 1 cut(s) 424
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 4 cut(s) 372, 759, 1013, 1405
AfiI CCNNNNNNNGG 6 cut(s) 21, 319, 809, 1618, 1653, 1733
AflIII ACRYGT 1 cut(s) 1279
AgsI TTSAA 5 cut(s) 914, 1034, 1415, 1691, 1697
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 3 cut(s) 83, 730, 1069
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 5 cut(s) 153, 260, 339, 371, 1156
Alw44I GTGCAC 1 cut(s) 465
AlwI GGATC 1 cut(s) 418
AlwNI CAGNNNCTG 1 cut(s) 1121
AoxI GGCC 5 cut(s) 81, 123, 219, 252, 1260
ApaLI GTGCAC 1 cut(s) 465
ApeKI GCWGC 8 cut(s) 229, 518, 955, 1457, 1460, 1539, 1574, 1615
ApoI RAATTY 1 cut(s) 1660
Asp700I GAANNNNTTC 2 cut(s) 308, 918
AspLEI GCGC 5 cut(s) 130, 197, 199, 217, 485
AsuC2I CCSGG 3 cut(s) 201, 320, 700
AsuHPI GGTGA 3 cut(s) 741, 756, 1503
AsuNHI GCTAGC 2 cut(s) 4, 1243
AxyI CCTNAGG 2 cut(s) 1515, 1532
BaeGI GKGCMC 1 cut(s) 469
BalI TGGCCA 2 cut(s) 221, 1262
BanI GGYRCC 1 cut(s) 845
BanII GRGCYC 2 cut(s) 535, 798
BarI GAAGNNNNNNTAC 2 cut(s) 900, 932
BbsI GAAGAC 2 cut(s) 54, 1708
Bbv12I GWGCWC 1 cut(s) 469
BbvCI CCTCAGC 1 cut(s) 980
BbvI GCAGC 8 cut(s) 241, 505, 967, 1444, 1472, 1526, 1561, 1602
BccI CCATC 5 cut(s) 263, 720, 769, 1312, 1636
BceAI ACGGC 2 cut(s) 109, 666
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 3 cut(s) 85, 732, 1071
BciVI GTATCC 1 cut(s) 1477
BcnI CCSGG 3 cut(s) 201, 320, 700
BcoDI GTCTC 5 cut(s) 153, 260, 339, 371, 1156
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 1244
BfmI CTRYAG 3 cut(s) 959, 1458, 1616
BfuI GTATCC 1 cut(s) 1477
BmcAI AGTACT 1 cut(s) 1013
Bme1390I CCNGG 6 cut(s) 85, 201, 320, 700, 732, 1071
BmiI GGNNCC 5 cut(s) 324, 534, 847, 1108, 1323
BmrFI CCNGG 6 cut(s) 85, 201, 320, 700, 732, 1071
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 5 cut(s) 689, 703, 1003, 1323, 1335
BmtI GCTAGC 2 cut(s) 8, 1247
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 54, 1708
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpmI CTGGAG 5 cut(s) 175, 753, 906, 1076, 1308
Bpu10I CCTNAGC 2 cut(s) 786, 980
BpuEI CTTGAG 1 cut(s) 974
BpuMI CCSGG 3 cut(s) 201, 320, 700
Bsa29I ATCGAT 1 cut(s) 1594
BsaAI YACGTR 1 cut(s) 374
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 6 cut(s) 84, 200, 318, 648, 698, 1436
BsaWI WCCGGW 2 cut(s) 108, 562
BsaXI ACNNNNNCTCC 4 cut(s) 577, 607, 1384, 1414
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 319, 809, 1618, 1653, 1733
Bse1I ACTGG 4 cut(s) 222, 327, 889, 1319
Bse21I CCTNAGG 2 cut(s) 1515, 1532
Bse3DI GCAATG 5 cut(s) 689, 716, 821, 906, 1584
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 3 cut(s) 85, 732, 1071
BseCI ATCGAT 1 cut(s) 1594
BseDI CCNNGG 6 cut(s) 84, 200, 318, 648, 698, 1436
BseGI GGATG 8 cut(s) 255, 683, 694, 712, 994, 1066, 1323, 1662
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 6 cut(s) 21, 319, 809, 1618, 1653, 1733
BseMI GCAATG 5 cut(s) 689, 716, 821, 906, 1584
BseMII CTCAG 4 cut(s) 994, 1332, 1506, 1551
BseNI ACTGG 4 cut(s) 222, 327, 889, 1319
BsePI GCGCGC 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 469
BseXI GCAGC 8 cut(s) 241, 505, 967, 1444, 1472, 1526, 1561, 1602
BseYI CCCAGC 3 cut(s) 798, 1611, 1727
BsgI GTGCAG 2 cut(s) 543, 1028
Bsh1236I CGCG 3 cut(s) 128, 197, 215
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 5 cut(s) 83, 125, 221, 254, 1262
BshNI GGYRCC 1 cut(s) 845
BshVI ATCGAT 1 cut(s) 1594
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 469
BsiSI CCGG 6 cut(s) 109, 200, 245, 320, 563, 700
BslFI GGGAC 5 cut(s) 309, 630, 645, 1208, 1610
BslI CCNNNNNNNGG 6 cut(s) 21, 319, 809, 1618, 1653, 1733
BsmAI GTCTC 5 cut(s) 153, 260, 339, 371, 1156
BsmBI CGTCTC 1 cut(s) 260
BsmFI GGGAC 5 cut(s) 309, 630, 645, 1208, 1610
BsnI GGCC 5 cut(s) 83, 125, 221, 254, 1262
Bsp1286I GDGCHC 3 cut(s) 469, 535, 798
Bsp143I GATC 3 cut(s) 406, 423, 1036
Bsp19I CCATGG 1 cut(s) 1436
BspACI CCGC 7 cut(s) 96, 126, 213, 227, 233, 259, 1760
BspANI GGCC 5 cut(s) 83, 125, 221, 254, 1262
BspCNI CTCAG 4 cut(s) 993, 1333, 1507, 1552
BspDI ATCGAT 1 cut(s) 1594
BspFNI CGCG 3 cut(s) 128, 197, 215
BspHI TCATGA 1 cut(s) 1093
BspLI GGNNCC 5 cut(s) 324, 534, 847, 1108, 1323
BspMAI CTGCAG 2 cut(s) 1462, 1620
BspOI GCTAGC 2 cut(s) 8, 1247
BspPI GGATC 1 cut(s) 418
BspT107I GGYRCC 1 cut(s) 845
BsrDI GCAATG 5 cut(s) 689, 716, 821, 906, 1584
BsrI ACTGG 4 cut(s) 222, 327, 889, 1319
BssECI CCNNGG 6 cut(s) 84, 200, 318, 648, 698, 1436
BssHII GCGCGC 1 cut(s) 195
BssMI GATC 3 cut(s) 406, 423, 1036
BssT1I CCWWGG 1 cut(s) 1436
Bst2UI CCWGG 3 cut(s) 85, 732, 1071
Bst4CI ACNGT 4 cut(s) 170, 382, 644, 1103
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 7 cut(s) 6, 197, 219, 739, 986, 1245, 1739
BstDEI CTNAG 6 cut(s) 786, 980, 1341, 1515, 1532, 1560
BstDSI CCRYGG 2 cut(s) 648, 1436
BstF5I GGATG 8 cut(s) 255, 683, 694, 712, 994, 1066, 1323, 1662
BstFNI CGCG 3 cut(s) 128, 197, 215
BstHHI GCGC 5 cut(s) 130, 197, 199, 217, 485
BstKTI GATC 3 cut(s) 409, 426, 1039
BstMAI GTCTC 5 cut(s) 153, 260, 339, 371, 1156
BstMBI GATC 3 cut(s) 406, 423, 1036
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 6 cut(s) 25, 241, 700, 852, 896, 961
BstNI CCWGG 3 cut(s) 85, 732, 1071
BstNSI RCATGY 2 cut(s) 1283, 1741
BstSCI CCNGG 6 cut(s) 83, 199, 318, 698, 730, 1069
BstSFI CTRYAG 3 cut(s) 959, 1458, 1616
BstSLI GKGCMC 1 cut(s) 469
BstUI CGCG 3 cut(s) 128, 197, 215
BstV1I GCAGC 8 cut(s) 241, 505, 967, 1444, 1472, 1526, 1561, 1602
BstV2I GAAGAC 2 cut(s) 54, 1708
BstX2I RGATCY 1 cut(s) 423
BstYI RGATCY 1 cut(s) 423
Bsu15I ATCGAT 1 cut(s) 1594
Bsu36I CCTNAGG 2 cut(s) 1515, 1532
BsuI GTATCC 1 cut(s) 1477
BsuRI GGCC 5 cut(s) 83, 125, 221, 254, 1262
BsuTUI ATCGAT 1 cut(s) 1594
BtgI CCRYGG 2 cut(s) 648, 1436
BtsCI GGATG 8 cut(s) 255, 683, 694, 712, 994, 1066, 1323, 1662
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 4 cut(s) 334, 387, 862, 1099
Cac8I GCNNGC 7 cut(s) 6, 197, 219, 739, 986, 1245, 1739
CaiI CAGNNNCTG 1 cut(s) 1121
CciI TCATGA 1 cut(s) 1093
CfoI GCGC 5 cut(s) 130, 197, 199, 217, 485
ClaI ATCGAT 1 cut(s) 1594
Csp6I GTAC 4 cut(s) 371, 758, 1012, 1404
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviQI GTAC 4 cut(s) 371, 758, 1012, 1404
DdeI CTNAG 6 cut(s) 786, 980, 1341, 1515, 1532, 1560
DpnI GATC 3 cut(s) 408, 425, 1038
DpnII GATC 3 cut(s) 406, 423, 1036
DraI TTTAAA 1 cut(s) 1471
DraIII CACNNNGTG 1 cut(s) 755
DrdI GACNNNNNNGTC 1 cut(s) 385
DseDI GACNNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 3 cut(s) 219, 252, 1260
EciI GGCGGA 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 1436
Eco24I GRGCYC 2 cut(s) 535, 798
Eco57I CTGAAG 1 cut(s) 424
Eco81I CCTNAGG 2 cut(s) 1515, 1532
EcoRII CCWGG 3 cut(s) 83, 730, 1069
EcoT14I CCWWGG 1 cut(s) 1436
EcoT22I ATGCAT 1 cut(s) 1350
EcoT38I GRGCYC 2 cut(s) 535, 798
ErhI CCWWGG 1 cut(s) 1436
Esp3I CGTCTC 1 cut(s) 260
FalI AAGNNNNNCTT 2 cut(s) 730, 762
FaqI GGGAC 5 cut(s) 309, 630, 645, 1208, 1610
FauI CCCGC 1 cut(s) 89
FokI GGATG 8 cut(s) 242, 670, 681, 699, 981, 1053, 1330, 1669
FriOI GRGCYC 2 cut(s) 535, 798
FspBI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 1244
FspI TGCGCA 1 cut(s) 484
GlaI GCGC 5 cut(s) 129, 196, 198, 216, 484
GsaI CCCAGC 3 cut(s) 802, 1615, 1731
GsuI CTGGAG 5 cut(s) 175, 753, 906, 1076, 1308
HaeIII GGCC 5 cut(s) 83, 125, 221, 254, 1262
HapII CCGG 6 cut(s) 109, 200, 245, 320, 563, 700
HhaI GCGC 5 cut(s) 130, 197, 199, 217, 485
Hin6I GCGC 5 cut(s) 128, 195, 197, 215, 483
HinP1I GCGC 5 cut(s) 128, 195, 197, 215, 483
HincII GTYRAC 1 cut(s) 1569
HindII GTYRAC 1 cut(s) 1569
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 9 cut(s) 209, 248, 400, 495, 586, 1223, 1288, 1681, 1723
HpaII CCGG 6 cut(s) 109, 200, 245, 320, 563, 700
HphI GGTGA 3 cut(s) 741, 756, 1503
Hpy166II GTNNAC 5 cut(s) 419, 467, 758, 1422, 1569
Hpy188I TCNGA 5 cut(s) 208, 610, 1342, 1366, 1769
Hpy188III TCNNGA 8 cut(s) 404, 540, 923, 1055, 1094, 1275, 1325, 1499
Hpy8I GTNNAC 5 cut(s) 419, 467, 758, 1422, 1569
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 8 cut(s) 73, 115, 674, 752, 756, 898, 1445, 1474
HpyCH4III ACNGT 4 cut(s) 170, 382, 644, 1103
HpyCH4IV ACGT 2 cut(s) 363, 373
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 241, 700, 852, 896, 961
HpyF3I CTNAG 6 cut(s) 786, 980, 1341, 1515, 1532, 1560
HpySE526I ACGT 2 cut(s) 363, 373
HspAI GCGC 5 cut(s) 128, 195, 197, 215, 483
Kzo9I GATC 3 cut(s) 406, 423, 1036
LmnI GCTCC 8 cut(s) 173, 194, 328, 538, 734, 887, 1106, 1327
Lsp1109I GCAGC 8 cut(s) 241, 505, 967, 1444, 1472, 1526, 1561, 1602
LweI GCATC 5 cut(s) 689, 703, 1003, 1323, 1335
MaeI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 1244
MaeII ACGT 2 cut(s) 363, 373
MaeIII GTNAC 1 cut(s) 1509
MalI GATC 3 cut(s) 408, 425, 1038
MboI GATC 3 cut(s) 406, 423, 1036
MboII GAAGA 4 cut(s) 54, 409, 1046, 1713
MfeI CAATTG 1 cut(s) 1686
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 3 cut(s) 469, 535, 798
MlsI TGGCCA 2 cut(s) 221, 1262
MluCI AATT 7 cut(s) 148, 1026, 1210, 1410, 1660, 1686, 1742
MluNI TGGCCA 2 cut(s) 221, 1262
MlyI GAGTC 2 cut(s) 580, 1675
MmeI TCCRAC 1 cut(s) 186
Mox20I TGGCCA 2 cut(s) 221, 1262
Mph1103I ATGCAT 1 cut(s) 1350
MroXI GAANNNNTTC 2 cut(s) 308, 918
MscI TGGCCA 2 cut(s) 221, 1262
MseI TTAA 4 cut(s) 807, 1080, 1470, 1745
MslI CAYNNNNRTG 2 cut(s) 14, 1736
Msp20I TGGCCA 2 cut(s) 221, 1262
MspA1I CMGCKG 1 cut(s) 1615
MspI CCGG 6 cut(s) 109, 200, 245, 320, 563, 700
MspR9I CCNGG 6 cut(s) 85, 201, 320, 700, 732, 1071
MunI CAATTG 1 cut(s) 1686
MvaI CCWGG 3 cut(s) 85, 732, 1071
MvnI CGCG 3 cut(s) 128, 197, 215
MwoI GCNNNNNNNGC 6 cut(s) 25, 241, 700, 852, 896, 961
NciI CCSGG 3 cut(s) 201, 320, 700
NcoI CCATGG 1 cut(s) 1436
NdeII GATC 3 cut(s) 406, 423, 1036
NheI GCTAGC 2 cut(s) 4, 1243
NlaIV GGNNCC 5 cut(s) 324, 534, 847, 1108, 1323
NmeAIII GCCGAG 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 1509
NsbI TGCGCA 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 1350
NspI RCATGY 2 cut(s) 1283, 1741
PaeI GCATGC 1 cut(s) 1741
PagI TCATGA 1 cut(s) 1093
PauI GCGCGC 1 cut(s) 195
PciI ACATGT 1 cut(s) 1279
PdmI GAANNNNTTC 2 cut(s) 308, 918
PfeI GAWTC 7 cut(s) 209, 248, 400, 495, 1223, 1288, 1723
PflFI GACNNNGTC 1 cut(s) 382
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 730
PleI GAGTC 2 cut(s) 580, 1675
PpsI GAGTC 2 cut(s) 580, 1675
Ppu21I YACGTR 1 cut(s) 374
PscI ACATGT 1 cut(s) 1279
PsiI TTATAA 1 cut(s) 1601
Psp1406I AACGTT 1 cut(s) 363
Psp6I CCWGG 3 cut(s) 83, 730, 1069
PspFI CCCAGC 3 cut(s) 798, 1611, 1727
PspGI CCWGG 3 cut(s) 83, 730, 1069
PspN4I GGNNCC 5 cut(s) 324, 534, 847, 1108, 1323
PsrI GAACNNNNNNTAC 2 cut(s) 613, 645
PstI CTGCAG 2 cut(s) 1462, 1620
PstNI CAGNNNCTG 1 cut(s) 1121
PsuI RGATCY 1 cut(s) 423
PsyI GACNNNGTC 1 cut(s) 382
PteI GCGCGC 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 1615
RsaI GTAC 4 cut(s) 372, 759, 1013, 1405
RsaNI GTAC 4 cut(s) 371, 758, 1012, 1404
RseI CAYNNNNRTG 2 cut(s) 14, 1736
SaqAI TTAA 4 cut(s) 807, 1080, 1470, 1745
Sau3AI GATC 3 cut(s) 406, 423, 1036
ScaI AGTACT 1 cut(s) 1013
SchI GAGTC 2 cut(s) 580, 1675
ScrFI CCNGG 6 cut(s) 85, 201, 320, 700, 732, 1071
SduI GDGCHC 3 cut(s) 469, 535, 798
SfaNI GCATC 5 cut(s) 689, 703, 1003, 1323, 1335
SfcI CTRYAG 3 cut(s) 959, 1458, 1616
SmiMI CAYNNNNRTG 2 cut(s) 14, 1736
SmlI CTYRAG 1 cut(s) 989
SmoI CTYRAG 1 cut(s) 989
SpeI ACTAGT 1 cut(s) 473
SphI GCATGC 1 cut(s) 1741
Sse9I AATT 7 cut(s) 148, 1026, 1210, 1410, 1660, 1686, 1742
SsiI CCGC 7 cut(s) 96, 126, 213, 227, 233, 259, 1760
SspI AATATT 1 cut(s) 1003
SspMI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 1244
StyD4I CCNGG 6 cut(s) 83, 199, 318, 698, 730, 1069
StyI CCWWGG 1 cut(s) 1436
TaaI ACNGT 4 cut(s) 170, 382, 644, 1103
TaiI ACGT 2 cut(s) 366, 376
TaqI TCGA 7 cut(s) 113, 134, 367, 409, 447, 667, 1594
TasI AATT 7 cut(s) 148, 1026, 1210, 1410, 1660, 1686, 1742
TatI WGTACW 2 cut(s) 1011, 1403
TauI GCSGC 3 cut(s) 128, 235, 1763
TfiI GAWTC 7 cut(s) 209, 248, 400, 495, 1223, 1288, 1723
Tru1I TTAA 4 cut(s) 807, 1080, 1470, 1745
Tru9I TTAA 4 cut(s) 807, 1080, 1470, 1745
TscAI CASTG 4 cut(s) 334, 387, 862, 1106
TseFI GTSAC 1 cut(s) 1509
TseI GCWGC 8 cut(s) 229, 518, 955, 1457, 1460, 1539, 1574, 1615
Tsp45I GTSAC 1 cut(s) 1509
TspGWI ACGGA 1 cut(s) 525
TspRI CASTG 4 cut(s) 334, 387, 862, 1106
Tth111I GACNNNGTC 1 cut(s) 382
Van91I CCANNNNNTGG 1 cut(s) 21
VneI GTGCAC 1 cut(s) 465
XapI RAATTY 1 cut(s) 1660
XceI RCATGY 2 cut(s) 1283, 1741
XcmI CCANNNNNNNNNTGG 1 cut(s) 1151
XmnI GAANNNNTTC 2 cut(s) 308, 918
XspI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 1244
ZrmI AGTACT 1 cut(s) 1013
Zsp2I ATGCAT 1 cut(s) 1350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.