RLG00000011383

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001271
Physical Location & Seq
Forward (+)
9024869 .. 9029451
4583 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011383

Sequence Viewer

Length: 2364 bp
ATGCCTTCTTCTTCTGCTCCATCTCAATCTCAATCTCAATCTCAGTCTCAGTCTCAATCTCAATCTCAATCTCATCAAAAACGAACCTTGTTTACATCTTCATCTTCTACTCCTGCTTCCTCCTCCTCCTCTTCCGTCTCAGCTCTGAGACCGGGGATCAACAGAAGCGGCAGTGATGTTAACAGCCCACGGCGGCTCACGCGCCAGAGGAAGCTGCGGTACCTCACGACCCCCGAGGATCTCGACTCTTACGGGGTCCCGGAGAAGTCTCGCTCATCGCCCACCTCGCCTGAATATTCGACCCGTAGATCACGCTCTCCGTGTGGCGAGTCGGCCCCAAGTGCTCGGTCCTCGGTCGGTGACTCGGCCCGAAAGTCATGCTGCTCTTCTTTCGTTGCTGGGCCTCAAGGCCCACACCCACAACCCTTGCCGCTTCCGGAGAACAACCGGAGACCTGACTGCGTCTCCAAAGATAACAACTTCTTCAGCCCTGTGGTCGGCAGGAAGAGCGTCGATCAAGCTGCCACAAGCGCATCATCACCGGCGAAATCTTCCAAGAACCGAAGCCGAGATGTAAACAATTCCGGCGGGGGTGGGGGTGGGGGTGGGGGTGGGAGTGGGGGTTTTAATTTCCGTTTGGGTTTGAATGTTCAATCTAGGACTACTAGGAGTGCTCCACAGACTGTGTTTAGCTCAAGTCATCATCCTGTTAGCCCACAAAGATCAAACACAGTGGATTTTTTGCCTTCTCAGTCACATGTTGCAGGCAACTATTATAACAGGGGAATGGGAATGGCAGTGTCACCGGAGGCTAACTTTGAGCGTGGAACTTACAACTTGAGCAGGAGTGCTCCAACCAGTGTTCTCTCAAGTCCTGCAGTTAGCCCGCGCCGATCAACCACTGGGGATCCTTTTCCTTTTTTCATGACTCATCAAGATCAATATCAGGATGGTGCTACCTTTCCCTCGTTGTTGTTTTCACCAAAGAAACCTACACCTAGTCCCACTCATTCTCCACTCCATAGCCCTACGCCTCGAAGTCCGCGCTTCAGCTCCAATGCTTCAAACTATGAGAGTAATAGTCCTGCCACTGCTCACCCGTTGCCTCTGCCTCCTGGAGCCGCAGTGCCAGCACAACCGCAGTCTATCACTATGCATCAAAATGTAGCACCAATGAAAGGTCAATGGCAGAAGGGGAAACTTATCGGACGGGGTACCTTTGGTAGTGTTTATCTTGCAACCAACCGTCACATGGCAGTTGCATTTATTTATTGTTATCTTCCCTTAGGTTTATATAAACCACCTAAATTGTCTGAATTAGGGATGTTTAGTCATGAAATTAAAGTCCTCCGTACGCTAAAGCATCCAAATATTGTGCAGTACTATGGTAGTGAAGTGATTGATGATCATTTCTACATATATTTAGAATATGTTCACCCTGGATCAATAAATAAATATGTCCAAGACCATTTTGGAGCTATGACTGAATCTGTAATCCGCAATTTTACCCGCCATATCCTCCGTGGGTTGGCTTTTTTGCATGACACGAAGACAATTCACAGGGATATTAAAGGTGCAAATTTGCTTGTTGATGCATCAGGCGTTGTCAAACTTGCTGATTTTGGGATGGCAAAACATCTTAGTGCGCATTCATACAATCTTTCTTTGAAGGGCAGCCCATACTGGATGGCTCCTGAGGTCATAAAGGCTGTGATACAGAATGATGGTGACCCTAATCTTGCTTTGGCTGTTGATATATGGAGCTTGGGTTGTACCATCATCGAGATGTTCAATGGAAAACCACCTTGGAGTGAATTTGAAGGGCCACAAGCTATGTTCAAGGTTCTGTACAGAACCCCGGATATACCAGAAAAATTATCTGCAGAAGGAAAGGAATTCCTCAGTTTGTGCTTCAAAAGGAACCCCGCAGAGAGGCCATCAGCTAAGAAGCTACTTGATCATCCGTTTGTACGAAATTCAAATGATCAAAATGTCTCATCTTATACTCCGGCTTTTTCTCTGGTAAACCTAATGGGTAAATTGAATAGCCCGACGGACCATATTAAGCAAAAACTTGGTCGAATGCTGCCCTTCTTCAAATTAAAGCATCCTGCATTGCTCGTCTTCGTGTTTTGTATCTCCATCCTTTTGCTCTTTGCTATAGAGCAATCATGGTTGATGGTTCTGGATGAAATGCATCAGGCAGCTGATTTGAGATTTTCCATTGCCTATTTTATCAGCATGCAGTGGGATTTAGATTTGGAAGCAGCTGGAGAGGCGAGGAAACCAGTAACCGCGTACCGCCTGAACCGGCGAGCAGCACCACCTGCAAACCTCTCGAGGGGTTTTCCACGTGGGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

788

Amino Acids

86.22

Weight (kDa)

9.79

Isoelectric Point (pI)

65.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 396 - 657 1e-60 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 399 - 652 1.1e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 777
AarI CACCTGC 1 cut(s) 2335
Acc16I TGCGCA 1 cut(s) 1647
Acc36I ACCTGC 1 cut(s) 2335
Acc65I GGTACC 2 cut(s) 219, 1214
AccB1I GGYRCC 2 cut(s) 219, 1214
AccII CGCG 4 cut(s) 202, 889, 1045, 2297
AccIII TCCGGA 1 cut(s) 436
AclWI GGATC 5 cut(s) 164, 246, 902, 915, 1450
AcsI RAATTY 4 cut(s) 1579, 1814, 1895, 1975
AcuI CTGAAG 2 cut(s) 469, 1033
AcvI CACGTG 1 cut(s) 2354
AfaI GTAC 8 cut(s) 221, 1216, 1354, 1382, 1774, 1850, 1971, 2300
AfiI CCNNNNNNNGG 6 cut(s) 497, 1178, 1252, 1528, 1932, 2341
AflIII ACRYGT 1 cut(s) 757
AjnI CCWGG 2 cut(s) 1114, 1438
AjuI GAANNNNNNNTTGG 2 cut(s) 1789, 1821
Alw21I GWGCWC 3 cut(s) 346, 676, 853
Alw26I GTCTC 8 cut(s) 51, 57, 142, 142, 273, 445, 469, 1999
AlwI GGATC 5 cut(s) 164, 246, 902, 915, 1450
Ama87I CYCGRG 2 cut(s) 233, 2338
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 6 cut(s) 333, 366, 401, 409, 1823, 1934
ApeKI GCWGC 8 cut(s) 214, 381, 521, 1674, 2086, 2204, 2267, 2318
ApoI RAATTY 4 cut(s) 1579, 1814, 1895, 1975
ArsI GACNNNNNNTTYG 2 cut(s) 2062, 2094
Asp700I GAANNNNTTC 1 cut(s) 1431
Asp718I GGTACC 2 cut(s) 219, 1214
AspLEI GCGC 5 cut(s) 204, 533, 891, 1047, 1648
AspS9I GGNCC 8 cut(s) 256, 334, 348, 367, 401, 410, 1823, 2056
AsuC2I CCSGG 3 cut(s) 153, 260, 1859
AsuHPI GGTGA 7 cut(s) 371, 531, 795, 972, 1088, 1427, 1739
AvaI CYCGRG 2 cut(s) 233, 2338
AvaII GGWCC 3 cut(s) 256, 348, 2056
AxyI CCTNAGG 2 cut(s) 1285, 1695
BamHI GGATCC 1 cut(s) 907
BanI GGYRCC 2 cut(s) 219, 1214
BarI GAAGNNNNNNTAC 2 cut(s) 203, 235
BbrPI CACGTG 1 cut(s) 2354
BbsI GAAGAC 2 cut(s) 1556, 2116
Bbv12I GWGCWC 3 cut(s) 346, 676, 853
BbvI GCAGC 8 cut(s) 201, 368, 508, 1686, 2073, 2216, 2279, 2330
BccI CCATC 9 cut(s) 28, 944, 1621, 1681, 1718, 1784, 1945, 2150, 2173
BceAI ACGGC 1 cut(s) 206
BcgI CGANNNNNNTGC 6 cut(s) 360, 394, 503, 537, 2319, 2353
BciT130I CCWGG 2 cut(s) 1116, 1440
BclI TGATCA 3 cut(s) 1405, 1957, 1984
BcnI CCSGG 3 cut(s) 153, 260, 1859
BcoDI GTCTC 8 cut(s) 51, 57, 142, 142, 273, 445, 469, 1999
BfaI CTAG 3 cut(s) 657, 666, 999
BfmI CTRYAG 3 cut(s) 876, 1881, 2160
BfuAI ACCTGC 1 cut(s) 2335
BmcAI AGTACT 1 cut(s) 1382
Bme1390I CCNGG 5 cut(s) 153, 260, 1116, 1440, 1859
Bme18I GGWCC 3 cut(s) 256, 348, 2056
BmeT110I CYCGRG 2 cut(s) 233, 2338
BmgT120I GGNCC 8 cut(s) 256, 334, 348, 367, 401, 410, 1823, 2056
BmiI GGNNCC 9 cut(s) 221, 257, 258, 336, 909, 1120, 1216, 1692, 1922
BmrFI CCNGG 5 cut(s) 153, 260, 1116, 1440, 1859
BmrI ACTGGG 1 cut(s) 912
BmsI GCATC 7 cut(s) 542, 1165, 1372, 1582, 1604, 2116, 2206
BmuI ACTGGG 1 cut(s) 912
BpiI GAAGAC 2 cut(s) 1556, 2116
BpmI CTGGAG 2 cut(s) 1137, 2292
BpuEI CTTGAG 4 cut(s) 390, 679, 853, 859
BpuMI CCSGG 3 cut(s) 153, 260, 1859
BsaAI YACGTR 1 cut(s) 2354
BsaBI GATNNNNATC 1 cut(s) 942
BsaI GGTCTC 2 cut(s) 142, 445
BsaJI CCNNGG 8 cut(s) 152, 188, 234, 351, 1438, 1522, 1805, 1857
BsaWI WCCGGW 3 cut(s) 436, 447, 805
Bsc4I CCNNNNNNNGG 6 cut(s) 497, 1178, 1252, 1528, 1932, 2341
Bse118I RCCGGY 2 cut(s) 541, 2310
Bse1I ACTGG 4 cut(s) 858, 907, 1688, 2288
Bse21I CCTNAGG 2 cut(s) 1285, 1695
Bse3DI GCAATG 2 cut(s) 2114, 2223
Bse8I GATNNNNATC 1 cut(s) 942
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 2 cut(s) 1116, 1440
BseDI CCNNGG 8 cut(s) 152, 188, 234, 351, 1438, 1522, 1805, 1857
BseJI GATNNNNATC 1 cut(s) 942
BseLI CCNNNNNNNGG 6 cut(s) 497, 1178, 1252, 1528, 1932, 2341
BseMI GCAATG 2 cut(s) 2114, 2223
BseMII CTCAG 7 cut(s) 56, 62, 137, 153, 764, 1686, 1915
BseNI ACTGG 4 cut(s) 858, 907, 1688, 2288
BseRI GAGGAG 3 cut(s) 112, 115, 118
BseXI GCAGC 8 cut(s) 201, 368, 508, 1686, 2073, 2216, 2279, 2330
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 1397
Bsh1236I CGCG 4 cut(s) 202, 889, 1045, 2297
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 6 cut(s) 335, 368, 403, 411, 1825, 1936
BshNI GGYRCC 2 cut(s) 219, 1214
BsiEI CGRYCG 1 cut(s) 357
BsiHKAI GWGCWC 3 cut(s) 346, 676, 853
BsiHKCI CYCGRG 2 cut(s) 233, 2338
BsiWI CGTACG 1 cut(s) 1352
BslFI GGGAC 2 cut(s) 242, 987
BslI CCNNNNNNNGG 6 cut(s) 497, 1178, 1252, 1528, 1932, 2341
BsmAI GTCTC 8 cut(s) 51, 57, 142, 142, 273, 445, 469, 1999
BsmBI CGTCTC 2 cut(s) 142, 469
BsmFI GGGAC 2 cut(s) 242, 987
BsmI GAATGC 2 cut(s) 1648, 2088
BsnI GGCC 6 cut(s) 335, 368, 403, 411, 1825, 1936
Bso31I GGTCTC 2 cut(s) 142, 445
BsoBI CYCGRG 2 cut(s) 233, 2338
Bsp1286I GDGCHC 3 cut(s) 346, 676, 853
Bsp13I TCCGGA 1 cut(s) 436
Bsp1407I TGTACA 1 cut(s) 1848
BspANI GGCC 6 cut(s) 335, 368, 403, 411, 1825, 1936
BspCNI CTCAG 7 cut(s) 55, 61, 138, 152, 763, 1687, 1914
BspEI TCCGGA 1 cut(s) 436
BspFNI CGCG 4 cut(s) 202, 889, 1045, 2297
BspHI TCATGA 2 cut(s) 924, 1333
BspLI GGNNCC 9 cut(s) 221, 257, 258, 336, 909, 1120, 1216, 1692, 1922
BspMAI CTGCAG 2 cut(s) 880, 1885
BspMI ACCTGC 1 cut(s) 2335
BspPI GGATC 5 cut(s) 164, 246, 902, 915, 1450
BspQI GCTCTTC 2 cut(s) 391, 500
BspT107I GGYRCC 2 cut(s) 219, 1214
BspTNI GGTCTC 2 cut(s) 142, 445
BsrDI GCAATG 2 cut(s) 2114, 2223
BsrFI RCCGGY 2 cut(s) 541, 2310
BsrGI TGTACA 1 cut(s) 1848
BsrI ACTGG 4 cut(s) 858, 907, 1688, 2288
BssAI RCCGGY 2 cut(s) 541, 2310
BssECI CCNNGG 8 cut(s) 152, 188, 234, 351, 1438, 1522, 1805, 1857
BssT1I CCWWGG 1 cut(s) 1805
Bst2UI CCWGG 2 cut(s) 1116, 1440
Bst4CI ACNGT 3 cut(s) 685, 733, 1247
Bst6I CTCTTC 3 cut(s) 136, 391, 500
BstAPI GCANNNNNTGC 1 cut(s) 2327
BstAUI TGTACA 1 cut(s) 1848
BstBAI YACGTR 1 cut(s) 2354
BstC8I GCNNGC 5 cut(s) 766, 887, 1131, 2243, 2316
BstDSI CCRYGG 2 cut(s) 188, 1522
BstEII GGTNACC 1 cut(s) 1727
BstFNI CGCG 4 cut(s) 202, 889, 1045, 2297
BstHHI GCGC 5 cut(s) 204, 533, 891, 1047, 1648
BstMAI GTCTC 8 cut(s) 51, 57, 142, 142, 273, 445, 469, 1999
BstMCI CGRYCG 1 cut(s) 357
BstMWI GCNNNNNNNGC 8 cut(s) 199, 286, 341, 507, 530, 1130, 2276, 2327
BstNI CCWGG 2 cut(s) 1116, 1440
BstNSI RCATGY 2 cut(s) 761, 2245
BstPI GGTNACC 1 cut(s) 1727
BstSCI CCNGG 5 cut(s) 151, 258, 1114, 1438, 1857
BstSFI CTRYAG 3 cut(s) 876, 1881, 2160
BstUI CGCG 4 cut(s) 202, 889, 1045, 2297
BstV1I GCAGC 8 cut(s) 201, 368, 508, 1686, 2073, 2216, 2279, 2330
BstV2I GAAGAC 2 cut(s) 1556, 2116
BstX2I RGATCY 2 cut(s) 238, 907
BstYI RGATCY 2 cut(s) 238, 907
Bsu36I CCTNAGG 2 cut(s) 1285, 1695
BsuRI GGCC 6 cut(s) 335, 368, 403, 411, 1825, 1936
BtgI CCRYGG 2 cut(s) 188, 1522
BtgZI GCGATG 1 cut(s) 261
BtsI GCAGTG 5 cut(s) 178, 804, 1089, 1131, 2252
BtsIMutI CAGTG 8 cut(s) 178, 738, 804, 865, 900, 1089, 1131, 2252
BveI ACCTGC 1 cut(s) 2335
Cac8I GCNNGC 5 cut(s) 766, 887, 1131, 2243, 2316
CciI TCATGA 2 cut(s) 924, 1333
CfoI GCGC 5 cut(s) 204, 533, 891, 1047, 1648
Cfr10I RCCGGY 2 cut(s) 541, 2310
Cfr13I GGNCC 8 cut(s) 256, 334, 348, 367, 401, 410, 1823, 2056
CseI GACGC 2 cut(s) 451, 499
Csp6I GTAC 8 cut(s) 220, 1215, 1353, 1381, 1773, 1849, 1970, 2299
CspCI CAANNNNNGTGG 6 cut(s) 408, 443, 714, 749, 1290, 1325
CviAII CATG 8 cut(s) 378, 758, 925, 1252, 1334, 1541, 2172, 2242
CviQI GTAC 8 cut(s) 220, 1215, 1353, 1381, 1773, 1849, 1970, 2299
Eam1104I CTCTTC 3 cut(s) 136, 391, 500
EarI CTCTTC 3 cut(s) 136, 391, 500
Eco130I CCWWGG 1 cut(s) 1805
Eco31I GGTCTC 2 cut(s) 142, 445
Eco47I GGWCC 3 cut(s) 256, 348, 2056
Eco57I CTGAAG 2 cut(s) 469, 1033
Eco72I CACGTG 1 cut(s) 2354
Eco81I CCTNAGG 2 cut(s) 1285, 1695
Eco88I CYCGRG 2 cut(s) 233, 2338
Eco91I GGTNACC 1 cut(s) 1727
EcoO109I RGGNCCY 1 cut(s) 256
EcoO65I GGTNACC 1 cut(s) 1727
EcoRI GAATTC 1 cut(s) 1895
EcoRII CCWGG 2 cut(s) 1114, 1438
EcoT14I CCWWGG 1 cut(s) 1805
EcoT22I ATGCAT 3 cut(s) 1158, 1597, 2199
ErhI CCWWGG 1 cut(s) 1805
Esp3I CGTCTC 2 cut(s) 142, 469
FaeI CATG 8 cut(s) 381, 761, 928, 1255, 1337, 1544, 2175, 2245
FaqI GGGAC 2 cut(s) 242, 987
FatI CATG 8 cut(s) 377, 757, 924, 1251, 1333, 1540, 2171, 2241
FauI CCCGC 4 cut(s) 581, 894, 1517, 1933
FbaI TGATCA 3 cut(s) 1405, 1957, 1984
FspAI RTGCGCAY 1 cut(s) 1647
FspBI CTAG 3 cut(s) 657, 666, 999
FspI TGCGCA 1 cut(s) 1647
GlaI GCGC 5 cut(s) 203, 532, 890, 1046, 1647
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 2 cut(s) 1137, 2292
HaeIII GGCC 6 cut(s) 335, 368, 403, 411, 1825, 1936
HgaI GACGC 2 cut(s) 451, 499
HhaI GCGC 5 cut(s) 204, 533, 891, 1047, 1648
Hin1II CATG 8 cut(s) 381, 761, 928, 1255, 1337, 1544, 2175, 2245
Hin6I GCGC 5 cut(s) 202, 531, 889, 1045, 1646
HinP1I GCGC 5 cut(s) 202, 531, 889, 1045, 1646
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 5 cut(s) 245, 329, 362, 928, 1487
HpaI GTTAAC 1 cut(s) 181
HphI GGTGA 7 cut(s) 371, 531, 795, 972, 1088, 1427, 1739
Hpy166II GTNNAC 5 cut(s) 93, 181, 577, 1435, 2026
Hpy188I TCNGA 3 cut(s) 147, 1208, 1315
Hpy8I GTNNAC 5 cut(s) 93, 181, 577, 1435, 2026
Hpy99I CGWCG 2 cut(s) 515, 2056
HpyAV CCTTC 7 cut(s) 15, 756, 1186, 1663, 1814, 1880, 2101
HpyCH4III ACNGT 3 cut(s) 685, 733, 1247
HpyCH4IV ACGT 1 cut(s) 2353
HpyF10VI GCNNNNNNNGC 8 cut(s) 199, 286, 341, 507, 530, 1130, 2276, 2327
HpySE526I ACGT 1 cut(s) 2353
Hsp92II CATG 8 cut(s) 381, 761, 928, 1255, 1337, 1544, 2175, 2245
HspAI GCGC 5 cut(s) 202, 531, 889, 1045, 1646
KflI GGGWCCC 1 cut(s) 256
Kpn2I TCCGGA 1 cut(s) 436
KpnI GGTACC 2 cut(s) 223, 1218
Ksp22I TGATCA 3 cut(s) 1405, 1957, 1984
KspAI GTTAAC 1 cut(s) 181
LguI GCTCTTC 2 cut(s) 391, 500
LmnI GCTCC 8 cut(s) 22, 679, 856, 1058, 1118, 1475, 1696, 1761
Lsp1109I GCAGC 8 cut(s) 201, 368, 508, 1686, 2073, 2216, 2279, 2330
LweI GCATC 7 cut(s) 542, 1165, 1372, 1582, 1604, 2116, 2206
MaeI CTAG 3 cut(s) 657, 666, 999
MaeII ACGT 1 cut(s) 2353
MaeIII GTNAC 6 cut(s) 359, 753, 801, 1247, 1727, 2290
MflI RGATCY 2 cut(s) 238, 907
MhlI GDGCHC 3 cut(s) 346, 676, 853
MlyI GAGTC 4 cut(s) 239, 338, 356, 922
MmeI TCCRAC 1 cut(s) 878
Mph1103I ATGCAT 3 cut(s) 1158, 1597, 2199
MroI TCCGGA 1 cut(s) 436
MroXI GAANNNNTTC 1 cut(s) 1431
MseI TTAA 6 cut(s) 180, 627, 1341, 1569, 2064, 2102
MslI CAYNNNNRTG 3 cut(s) 1161, 1641, 1784
MspA1I CMGCKG 2 cut(s) 2207, 2270
MspR9I CCNGG 5 cut(s) 153, 260, 1116, 1440, 1859
Mva1269I GAATGC 2 cut(s) 1648, 2088
MvaI CCWGG 2 cut(s) 1116, 1440
MvnI CGCG 4 cut(s) 202, 889, 1045, 2297
MwoI GCNNNNNNNGC 8 cut(s) 199, 286, 341, 507, 530, 1130, 2276, 2327
NciI CCSGG 3 cut(s) 153, 260, 1859
NlaIII CATG 8 cut(s) 381, 761, 928, 1255, 1337, 1544, 2175, 2245
NlaIV GGNNCC 9 cut(s) 221, 257, 258, 336, 909, 1120, 1216, 1692, 1922
NmeAIII GCCGAG 2 cut(s) 344, 593
NmuCI GTSAC 5 cut(s) 359, 753, 801, 1247, 1727
NsbI TGCGCA 1 cut(s) 1647
NsiI ATGCAT 3 cut(s) 1158, 1597, 2199
NspI RCATGY 2 cut(s) 761, 2245
PaeI GCATGC 1 cut(s) 2245
PaeR7I CTCGAG 1 cut(s) 2338
PagI TCATGA 2 cut(s) 924, 1333
PaqCI CACCTGC 1 cut(s) 2335
PciI ACATGT 1 cut(s) 757
PciSI GCTCTTC 2 cut(s) 391, 500
PctI GAATGC 2 cut(s) 1648, 2088
PdmI GAANNNNTTC 1 cut(s) 1431
PfeI GAWTC 1 cut(s) 1487
Pfl23II CGTACG 1 cut(s) 1352
PflFI GACNNNGTC 1 cut(s) 461
PfoI TCCNGGA 2 cut(s) 258, 1114
PleI GAGTC 4 cut(s) 239, 337, 356, 922
PmaCI CACGTG 1 cut(s) 2354
PmlI CACGTG 1 cut(s) 2354
PpsI GAGTC 4 cut(s) 239, 337, 356, 922
Ppu21I YACGTR 1 cut(s) 2354
PpuMI RGGWCCY 1 cut(s) 256
PscI ACATGT 1 cut(s) 757
PsiI TTATAA 1 cut(s) 777
Psp5II RGGWCCY 1 cut(s) 256
Psp6I CCWGG 2 cut(s) 1114, 1438
PspCI CACGTG 1 cut(s) 2354
PspEI GGTNACC 1 cut(s) 1727
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 2 cut(s) 1114, 1438
PspLI CGTACG 1 cut(s) 1352
PspN4I GGNNCC 9 cut(s) 221, 257, 258, 336, 909, 1120, 1216, 1692, 1922
PspPI GGNCC 8 cut(s) 256, 334, 348, 367, 401, 410, 1823, 2056
PspPPI RGGWCCY 1 cut(s) 256
PsrI GAACNNNNNNTAC 2 cut(s) 76, 108
PstI CTGCAG 2 cut(s) 880, 1885
PsuI RGATCY 2 cut(s) 238, 907
PsyI GACNNNGTC 1 cut(s) 461
PvuII CAGCTG 2 cut(s) 2207, 2270
RsaI GTAC 8 cut(s) 221, 1216, 1354, 1382, 1774, 1850, 1971, 2300
RsaNI GTAC 8 cut(s) 220, 1215, 1353, 1381, 1773, 1849, 1970, 2299
RseI CAYNNNNRTG 3 cut(s) 1161, 1641, 1784
SapI GCTCTTC 2 cut(s) 391, 500
SaqAI TTAA 6 cut(s) 180, 627, 1341, 1569, 2064, 2102
Sau96I GGNCC 8 cut(s) 256, 334, 348, 367, 401, 410, 1823, 2056
ScaI AGTACT 1 cut(s) 1382
SchI GAGTC 4 cut(s) 239, 338, 356, 922
ScrFI CCNGG 5 cut(s) 153, 260, 1116, 1440, 1859
SduI GDGCHC 3 cut(s) 346, 676, 853
SfaNI GCATC 7 cut(s) 542, 1165, 1372, 1582, 1604, 2116, 2206
SfcI CTRYAG 3 cut(s) 876, 1881, 2160
Sfr274I CTCGAG 1 cut(s) 2338
SgrAI CRCCGGYG 1 cut(s) 541
SinI GGWCC 3 cut(s) 256, 348, 2056
SlaI CTCGAG 1 cut(s) 2338
SmiMI CAYNNNNRTG 3 cut(s) 1161, 1641, 1784
SmlI CTYRAG 5 cut(s) 405, 694, 838, 868, 2338
SmoI CTYRAG 5 cut(s) 405, 694, 838, 868, 2338
SphI GCATGC 1 cut(s) 2245
SspI AATATT 2 cut(s) 296, 1372
SspMI CTAG 3 cut(s) 657, 666, 999
StyD4I CCNGG 5 cut(s) 151, 258, 1114, 1438, 1857
StyI CCWWGG 1 cut(s) 1805
TaaI ACNGT 3 cut(s) 685, 733, 1247
TaiI ACGT 1 cut(s) 2356
TaqI TCGA 7 cut(s) 243, 299, 513, 1036, 1782, 2080, 2339
TaqII GACCGA 2 cut(s) 336, 343
TatI WGTACW 2 cut(s) 1380, 1848
TauI GCSGC 4 cut(s) 171, 196, 433, 1124
TfiI GAWTC 1 cut(s) 1487
Tru1I TTAA 6 cut(s) 180, 627, 1341, 1569, 2064, 2102
Tru9I TTAA 6 cut(s) 180, 627, 1341, 1569, 2064, 2102
TscAI CASTG 8 cut(s) 178, 738, 804, 865, 907, 1096, 1131, 2252
TseFI GTSAC 5 cut(s) 359, 753, 801, 1247, 1727
TseI GCWGC 8 cut(s) 214, 381, 521, 1674, 2086, 2204, 2267, 2318
Tsp45I GTSAC 5 cut(s) 359, 753, 801, 1247, 1727
TspDTI ATGAA 6 cut(s) 90, 913, 1190, 1350, 1641, 2205
TspGWI ACGGA 7 cut(s) 124, 309, 623, 1340, 1511, 1953, 2069
TspRI CASTG 8 cut(s) 178, 738, 804, 865, 907, 1096, 1131, 2252
Tth111I GACNNNGTC 1 cut(s) 461
VpaK11BI GGWCC 3 cut(s) 256, 348, 2056
XapI RAATTY 4 cut(s) 1579, 1814, 1895, 1975
XceI RCATGY 2 cut(s) 761, 2245
XcmI CCANNNNNNNNNTGG 3 cut(s) 1249, 1469, 1520
XhoI CTCGAG 1 cut(s) 2338
XmnI GAANNNNTTC 1 cut(s) 1431
XspI CTAG 3 cut(s) 657, 666, 999
ZrmI AGTACT 1 cut(s) 1382
Zsp2I ATGCAT 3 cut(s) 1158, 1597, 2199
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.