MD15G1035900.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001271
Physical Location & Seq
Forward (+)
2515652 .. 2519958
4307 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1035900.v1.1.491

Sequence Viewer

Length: 2064 bp
ATGCCTTCTACCTCGTTTTCTCATCACAAACCCTGCAGAGCTACAACCTCCCCCTCCTCCAACTCCTCCTCCCCTGTTTTTTCTTCAGCTGCTGCTTGCTCTGTTTCTGCTCCGAAACAGGACAGTGGTGGTGGTGGGGGCAGCTGCGAGCAGAGCCCGCGGCGGCTCACCCGGCAAAGAAAGCTCCGCCACATCAACAACCAGGATTTCTACCTCCCTGATAAGTCTTACTCCTCGCCCGCTTCGCCGGACTATTCGACCCAAAAGTCAAGATCGCCGGGTCGTGGGTCCAAGTCAAACCACTGGTCTTCTGCGCCGGCTCCGCAGCCCTTGCCCCTTCCTGAATCTCCCGTGATCCGGAAACCTGAATCTGCTAAAAATGGGTTCTTTGGCCCCAGGCAGGTCAAAGGTAAAGCTGTCACAAGCTCAGGATCGGCATTGCCGAGTTCTTATTCGAGTCAAGTTGCCACAACCTCAGGAGGATCAGCAGCAGCAGTGAGTTTTTATTCAAACAATCGTCGAGGGGCACTCTCCCAAGATGCTAACAATGGTGGCGGTTCGCTGAGTTCTTGTTCTAGTCAAGTTGCCACAACCTCAGGAGGATCATCAGCACCAGTGAGTTCTTATTCGAACAATCGTCGAGGGGCACTCTCCCAAGATGCTAACAATGGTGGGGGTTTCTTTATTAACTTGGGTCTGAATATTCTTTCTAGGAAAGCGCAGGCAAATGGTATATCAAGCCATCCTTTTAGTCCCCAAAGATCGAAAACTGTCGATAGTTATCCTCTACTTGTTGCTCCCACTTCACCCGTTAATTGTTCAAGCAACTGCCATAGGGGAACGCCACACGAATATGAAAGTTGCAACATGAGCAGGAGTGCTCCAACAAGTGCTTTTTCAAGTCCTGCAGTTAGCCCCAGGAGATCAAACACCGCTGGGGATTTTTTTTATTCTTTTGGGGCTCCCCACGATCAAAATCTGGATGTCTTTCCTCCAAGATTATCACCAAATAGACCTGCTCATAGTCCCACCCATTCTCCTACATCTCGAAGTCCATGCTTCAACCAGAAAAGTTCAAATGGAAATGCATTTCTTTTGCCTTCTTTAGAGAGGGCTGAAAGTAGTGGTCAAGTCAGTGCCCACCCTTTGCCTCTGCCACCTCCACCACAGCAATCTGTTATGCACCACAATGCAGAAGCTCTATCCTTATCATCAATGAAAGGTCAATGGCAGAAGGGAAAACTTATTGGGCGCGGCACCTTTGGTAGTGTGTATCTCGCAACCAACCGAGAAACCGGGGCCTTGTGTGCAATGAAGGAAGTTGATCTTATTCCTGATGATCCTAAAGCTGCTGAATGTATAAAGCAATTGGAGCAGGAAATTAAAGTCCTCCGCACGCTAAAGCATCCAAATATTGTGCAGTATTATGGTAGTGAAATGACTGAAGATCACTTCTACATATATTTGGAATATGTTCATCCTGGATCGATTAACAAATACGTCCAAGACCATATTGGAACTATGACAGAATCTGTAGTTCGCAGTTTTACTCGCCATATCCTCTCTGGGTTGGCTTTCTTGCACAGCACAAAGACAATTCACAGAGATATAAAAGGTGCAAATTTGCTTGTTGATGCATCAGGCGTTGTTAAACTTGCCGATTTTGGGGTGGCAAAACATCTCAATGGGCAATCGTACAATCTTTCTTTGAAGGGCAGTCCATACTGGATGGCTCCTGAGGTGATAAGGGCTGCAATGCAGAACAGTGCTGCTCCTGATCTTGCTTTGGCCGTCGATATATGGAGCTTGGGTTGTACTATTGTTGAGATGTTCAATGGAAGACCTCCGTGGAGTGAATTTACAGGACCTCAAGCTATGTTCAAGGTTCTAAACACCATCCCGGATATACCAGAAACATTGTCTGCAGAGGGAAAGGACTTCCTTCATTTGTGCTTTCGTAGGAACCCTGCAGAGAGACCGTTAGCTAGTAAGCTACTTGAGCATCCGTTTGTTCGAAATTCATACGATAAATTGGATGGTCCGAAGGACCACATTAATCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

688

Amino Acids

73.9

Weight (kDa)

9.36

Isoelectric Point (pI)

63.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 410 - 671 4.1e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 413 - 667 2.8e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 265
Acc36I ACCTGC 2 cut(s) 391, 1024
AccB1I GGYRCC 1 cut(s) 1256
AccII CGCG 2 cut(s) 160, 1254
AccIII TCCGGA 1 cut(s) 357
AclWI GGATC 6 cut(s) 349, 439, 490, 610, 1334, 1492
AcoI YGGCCR 1 cut(s) 1788
AcsI RAATTY 3 cut(s) 1621, 1856, 2017
AcuI CTGAAG 2 cut(s) 69, 1464
AfaI GTAC 2 cut(s) 1697, 1816
AfiI CCNNNNNNNGG 4 cut(s) 284, 357, 400, 1665
AgsI TTSAA 8 cut(s) 510, 822, 900, 1063, 1077, 1711, 1834, 1882
AjnI CCWGG 4 cut(s) 201, 395, 917, 1480
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 1 cut(s) 1969
AlwI GGATC 6 cut(s) 349, 439, 490, 610, 1334, 1492
AlwNI CAGNNNCTG 2 cut(s) 92, 1532
Aor13HI TCCGGA 1 cut(s) 357
AoxI GGCC 3 cut(s) 391, 1299, 1788
ApoI RAATTY 3 cut(s) 1621, 1856, 2017
ArsI GACNNNNNNTTYG 2 cut(s) 290, 322
AseI ATTAAT 1 cut(s) 2055
Asp700I GAANNNNTTC 1 cut(s) 1473
AspLEI GCGC 3 cut(s) 316, 721, 1254
AspS9I GGNCC 6 cut(s) 288, 392, 1299, 1865, 2039, 2047
AsuC2I CCSGG 4 cut(s) 172, 279, 1297, 1901
AsuHPI GGTGA 4 cut(s) 160, 798, 996, 1753
AsuII TTCGAA 2 cut(s) 629, 2014
AvaII GGWCC 4 cut(s) 288, 1865, 2039, 2047
AxyI CCTNAGG 3 cut(s) 475, 595, 1737
BaeGI GKGCMC 3 cut(s) 529, 649, 1141
BanI GGYRCC 1 cut(s) 1256
BanII GRGCYC 2 cut(s) 158, 964
BbsI GAAGAC 2 cut(s) 300, 1846
Bbv12I GWGCWC 1 cut(s) 883
BccI CCATC 4 cut(s) 750, 1723, 1904, 2030
BceAI ACGGC 1 cut(s) 1775
BcgI CGANNNNNNTGC 2 cut(s) 1038, 1072
BciT130I CCWGG 4 cut(s) 203, 397, 919, 1482
BcnI CCSGG 4 cut(s) 172, 279, 1297, 1901
BcoDI GTCTC 1 cut(s) 1969
BfaI CTAG 3 cut(s) 576, 711, 1986
BfmI CTRYAG 5 cut(s) 34, 906, 1533, 1923, 1968
BfuAI ACCTGC 2 cut(s) 391, 1024
Bme1390I CCNGG 8 cut(s) 172, 203, 279, 397, 919, 1297, 1482, 1901
Bme18I GGWCC 4 cut(s) 288, 1865, 2039, 2047
BmgT120I GGNCC 6 cut(s) 288, 392, 1299, 1865, 2039, 2047
BmiI GGNNCC 8 cut(s) 289, 321, 394, 963, 1258, 1300, 1734, 1964
BmrFI CCNGG 8 cut(s) 172, 203, 279, 397, 919, 1297, 1482, 1901
BmsI GCATC 6 cut(s) 529, 649, 1414, 1624, 1646, 2011
BpiI GAAGAC 2 cut(s) 300, 1846
BplI GAGNNNNNCTC 4 cut(s) 513, 545, 633, 665
Bpu10I CCTNAGC 1 cut(s) 427
Bpu14I TTCGAA 2 cut(s) 629, 2014
BpuEI CTTGAG 2 cut(s) 1854, 2018
BpuMI CCSGG 4 cut(s) 172, 279, 1297, 1901
Bsa29I ATCGAT 1 cut(s) 1487
BsaBI GATNNNNATC 2 cut(s) 780, 975
BsaI GGTCTC 1 cut(s) 1969
BsaJI CCNNGG 5 cut(s) 158, 395, 917, 1296, 1847
BsaWI WCCGGW 1 cut(s) 357
BsaXI ACNNNNNCTCC 6 cut(s) 53, 83, 1021, 1051, 1795, 1825
Bsc4I CCNNNNNNNGG 4 cut(s) 284, 357, 400, 1665
Bse118I RCCGGY 1 cut(s) 316
Bse1I ACTGG 3 cut(s) 308, 614, 1730
Bse21I CCTNAGG 3 cut(s) 475, 595, 1737
Bse3DI GCAATG 3 cut(s) 437, 1317, 1761
Bse8I GATNNNNATC 2 cut(s) 780, 975
BseAI TCCGGA 1 cut(s) 357
BseBI CCWGG 4 cut(s) 203, 397, 919, 1482
BseCI ATCGAT 1 cut(s) 1487
BseDI CCNNGG 5 cut(s) 158, 395, 917, 1296, 1847
BseGI GGATG 8 cut(s) 742, 988, 1405, 1477, 1734, 1896, 2002, 2041
BseJI GATNNNNATC 2 cut(s) 780, 975
BseLI CCNNNNNNNGG 4 cut(s) 284, 357, 400, 1665
BseMI GCAATG 3 cut(s) 437, 1317, 1761
BseMII CTCAG 5 cut(s) 441, 489, 554, 609, 1728
BseNI ACTGG 3 cut(s) 308, 614, 1730
BseRI GAGGAG 4 cut(s) 46, 55, 58, 223
BseSI GKGCMC 3 cut(s) 529, 649, 1141
BseYI CCCAGC 1 cut(s) 935
BsgI GTGCAG 1 cut(s) 1439
Bsh1236I CGCG 2 cut(s) 160, 1254
BshFI GGCC 3 cut(s) 393, 1301, 1790
BshNI GGYRCC 1 cut(s) 1256
BshVI ATCGAT 1 cut(s) 1487
BsiHKAI GWGCWC 1 cut(s) 883
BsiSI CCGG 7 cut(s) 172, 248, 278, 317, 358, 1296, 1901
BslFI GGGAC 2 cut(s) 738, 1011
BslI CCNNNNNNNGG 4 cut(s) 284, 357, 400, 1665
BsmAI GTCTC 1 cut(s) 1969
BsmFI GGGAC 2 cut(s) 738, 1011
BsnI GGCC 3 cut(s) 393, 1301, 1790
Bso31I GGTCTC 1 cut(s) 1969
Bsp119I TTCGAA 2 cut(s) 629, 2014
Bsp1286I GDGCHC 6 cut(s) 158, 529, 649, 883, 964, 1141
Bsp13I TCCGGA 1 cut(s) 357
BspANI GGCC 3 cut(s) 393, 1301, 1790
BspCNI CTCAG 5 cut(s) 440, 488, 555, 608, 1729
BspDI ATCGAT 1 cut(s) 1487
BspEI TCCGGA 1 cut(s) 357
BspFNI CGCG 2 cut(s) 160, 1254
BspLI GGNNCC 8 cut(s) 289, 321, 394, 963, 1258, 1300, 1734, 1964
BspMAI CTGCAG 4 cut(s) 38, 910, 1927, 1972
BspMI ACCTGC 2 cut(s) 391, 1024
BspPI GGATC 6 cut(s) 349, 439, 490, 610, 1334, 1492
BspT104I TTCGAA 2 cut(s) 629, 2014
BspT107I GGYRCC 1 cut(s) 1256
BspTNI GGTCTC 1 cut(s) 1969
BsrDI GCAATG 3 cut(s) 437, 1317, 1761
BsrFI RCCGGY 1 cut(s) 316
BsrI ACTGG 3 cut(s) 308, 614, 1730
BssAI RCCGGY 1 cut(s) 316
BssECI CCNNGG 5 cut(s) 158, 395, 917, 1296, 1847
Bst2UI CCWGG 4 cut(s) 203, 397, 919, 1482
Bst4CI ACNGT 4 cut(s) 125, 772, 1766, 1980
BstAPI GCANNNNNTGC 1 cut(s) 331
BstBI TTCGAA 2 cut(s) 629, 2014
BstC8I GCNNGC 7 cut(s) 97, 149, 158, 240, 318, 723, 1397
BstDEI CTNAG 5 cut(s) 427, 475, 563, 595, 1737
BstDSI CCRYGG 2 cut(s) 158, 1847
BstF5I GGATG 8 cut(s) 742, 988, 1405, 1477, 1734, 1896, 2002, 2041
BstFNI CGCG 2 cut(s) 160, 1254
BstHHI GCGC 3 cut(s) 316, 721, 1254
BstMAI GTCTC 1 cut(s) 1969
BstNI CCWGG 4 cut(s) 203, 397, 919, 1482
BstSCI CCNGG 8 cut(s) 170, 201, 277, 395, 917, 1295, 1480, 1899
BstSFI CTRYAG 5 cut(s) 34, 906, 1533, 1923, 1968
BstSLI GKGCMC 3 cut(s) 529, 649, 1141
BstUI CGCG 2 cut(s) 160, 1254
BstV2I GAAGAC 2 cut(s) 300, 1846
Bsu15I ATCGAT 1 cut(s) 1487
Bsu36I CCTNAGG 3 cut(s) 475, 595, 1737
BsuRI GGCC 3 cut(s) 393, 1301, 1790
BsuTUI ATCGAT 1 cut(s) 1487
BtgI CCRYGG 2 cut(s) 158, 1847
BtsCI GGATG 8 cut(s) 742, 988, 1405, 1477, 1734, 1896, 2002, 2041
BtsI GCAGTG 1 cut(s) 501
BtsIMutI CAGTG 6 cut(s) 130, 301, 501, 621, 1141, 1771
BveI ACCTGC 2 cut(s) 391, 1024
Cac8I GCNNGC 7 cut(s) 97, 149, 158, 240, 318, 723, 1397
CaiI CAGNNNCTG 2 cut(s) 92, 1532
CfoI GCGC 3 cut(s) 316, 721, 1254
Cfr10I RCCGGY 1 cut(s) 316
Cfr13I GGNCC 6 cut(s) 288, 392, 1299, 1865, 2039, 2047
Cfr42I CCGCGG 1 cut(s) 161
ClaI ATCGAT 1 cut(s) 1487
Csp6I GTAC 2 cut(s) 1696, 1815
CviAII CATG 2 cut(s) 868, 1056
CviQI GTAC 2 cut(s) 1696, 1815
DdeI CTNAG 5 cut(s) 427, 475, 563, 595, 1737
DrdI GACNNNNNNGTC 1 cut(s) 265
DseDI GACNNNNNNGTC 1 cut(s) 265
EaeI YGGCCR 1 cut(s) 1788
EciI GGCGGA 1 cut(s) 176
Eco24I GRGCYC 2 cut(s) 158, 964
Eco31I GGTCTC 1 cut(s) 1969
Eco47I GGWCC 4 cut(s) 288, 1865, 2039, 2047
Eco57I CTGAAG 2 cut(s) 69, 1464
Eco81I CCTNAGG 3 cut(s) 475, 595, 1737
EcoO109I RGGNCCY 2 cut(s) 1299, 1865
EcoRII CCWGG 4 cut(s) 201, 395, 917, 1480
EcoT22I ATGCAT 2 cut(s) 1090, 1639
EcoT38I GRGCYC 2 cut(s) 158, 964
FaeI CATG 2 cut(s) 871, 1059
FalI AAGNNNNNCTT 4 cut(s) 730, 762, 1311, 1343
FaqI GGGAC 2 cut(s) 738, 1011
FatI CATG 2 cut(s) 867, 1055
FauI CCCGC 2 cut(s) 165, 247
FokI GGATG 8 cut(s) 729, 995, 1392, 1464, 1741, 1883, 1989, 2048
FriOI GRGCYC 2 cut(s) 158, 964
FspBI CTAG 3 cut(s) 576, 711, 1986
GlaI GCGC 3 cut(s) 315, 720, 1253
GsaI CCCAGC 1 cut(s) 939
HaeIII GGCC 3 cut(s) 393, 1301, 1790
HapII CCGG 7 cut(s) 172, 248, 278, 317, 358, 1296, 1901
HhaI GCGC 3 cut(s) 316, 721, 1254
Hin1II CATG 2 cut(s) 871, 1059
Hin6I GCGC 3 cut(s) 314, 719, 1252
HinP1I GCGC 3 cut(s) 314, 719, 1252
HinfI GANTC 4 cut(s) 344, 368, 457, 1529
HpaII CCGG 7 cut(s) 172, 248, 278, 317, 358, 1296, 1901
HphI GGTGA 4 cut(s) 160, 798, 996, 1753
Hpy188I TCNGA 3 cut(s) 114, 699, 2043
Hpy99I CGWCG 3 cut(s) 522, 642, 1796
HpyAV CCTTC 8 cut(s) 15, 347, 1110, 1228, 1309, 1705, 1952, 2038
HpyCH4III ACNGT 4 cut(s) 125, 772, 1766, 1980
HpyCH4IV ACGT 1 cut(s) 1500
HpyF3I CTNAG 5 cut(s) 427, 475, 563, 595, 1737
HpySE526I ACGT 1 cut(s) 1500
Hsp92II CATG 2 cut(s) 871, 1059
HspAI GCGC 3 cut(s) 314, 719, 1252
Kpn2I TCCGGA 1 cut(s) 357
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
KspI CCGCGG 1 cut(s) 161
LweI GCATC 6 cut(s) 529, 649, 1414, 1624, 1646, 2011
MaeI CTAG 3 cut(s) 576, 711, 1986
MaeII ACGT 1 cut(s) 1500
MaeIII GTNAC 1 cut(s) 418
MboII GAAGA 4 cut(s) 75, 300, 1457, 1851
MfeI CAATTG 1 cut(s) 1367
MhlI GDGCHC 6 cut(s) 158, 529, 649, 883, 964, 1141
MluCI AATT 8 cut(s) 814, 1367, 1380, 1596, 1621, 1856, 2017, 2030
MlyI GAGTC 1 cut(s) 466
MmeI TCCRAC 2 cut(s) 84, 908
Mph1103I ATGCAT 2 cut(s) 1090, 1639
MroI TCCGGA 1 cut(s) 357
MroNI GCCGGC 1 cut(s) 316
MroXI GAANNNNTTC 1 cut(s) 1473
MseI TTAA 7 cut(s) 687, 813, 1383, 1491, 1650, 2055, 2062
MslI CAYNNNNRTG 3 cut(s) 852, 1188, 1683
MspA1I CMGCKG 4 cut(s) 89, 144, 160, 935
MspI CCGG 7 cut(s) 172, 248, 278, 317, 358, 1296, 1901
MspR9I CCNGG 8 cut(s) 172, 203, 279, 397, 919, 1297, 1482, 1901
MunI CAATTG 1 cut(s) 1367
MvaI CCWGG 4 cut(s) 203, 397, 919, 1482
MvnI CGCG 2 cut(s) 160, 1254
NaeI GCCGGC 1 cut(s) 318
NciI CCSGG 4 cut(s) 172, 279, 1297, 1901
NgoMIV GCCGGC 1 cut(s) 316
NlaIII CATG 2 cut(s) 871, 1059
NlaIV GGNNCC 8 cut(s) 289, 321, 394, 963, 1258, 1300, 1734, 1964
NmeAIII GCCGAG 1 cut(s) 468
NmuCI GTSAC 1 cut(s) 418
NsiI ATGCAT 2 cut(s) 1090, 1639
NspV TTCGAA 2 cut(s) 629, 2014
PcsI WCGNNNNNNNCGW 1 cut(s) 440
PdiI GCCGGC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 1473
PfeI GAWTC 3 cut(s) 344, 368, 1529
PfoI TCCNGGA 2 cut(s) 1480, 1899
PleI GAGTC 1 cut(s) 465
PpsI GAGTC 1 cut(s) 465
PpuMI RGGWCCY 1 cut(s) 1865
PshBI ATTAAT 1 cut(s) 2055
Psp5II RGGWCCY 1 cut(s) 1865
Psp6I CCWGG 4 cut(s) 201, 395, 917, 1480
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 4 cut(s) 201, 395, 917, 1480
PspN4I GGNNCC 8 cut(s) 289, 321, 394, 963, 1258, 1300, 1734, 1964
PspPI GGNCC 6 cut(s) 288, 392, 1299, 1865, 2039, 2047
PspPPI RGGWCCY 1 cut(s) 1865
PstI CTGCAG 4 cut(s) 38, 910, 1927, 1972
PstNI CAGNNNCTG 2 cut(s) 92, 1532
PvuII CAGCTG 2 cut(s) 89, 144
RsaI GTAC 2 cut(s) 1697, 1816
RsaNI GTAC 2 cut(s) 1696, 1815
RseI CAYNNNNRTG 3 cut(s) 852, 1188, 1683
SacII CCGCGG 1 cut(s) 161
SaqAI TTAA 7 cut(s) 687, 813, 1383, 1491, 1650, 2055, 2062
Sau96I GGNCC 6 cut(s) 288, 392, 1299, 1865, 2039, 2047
SchI GAGTC 1 cut(s) 466
ScrFI CCNGG 8 cut(s) 172, 203, 279, 397, 919, 1297, 1482, 1901
SduI GDGCHC 6 cut(s) 158, 529, 649, 883, 964, 1141
SfaNI GCATC 6 cut(s) 529, 649, 1414, 1624, 1646, 2011
SfcI CTRYAG 5 cut(s) 34, 906, 1533, 1923, 1968
Sfr303I CCGCGG 1 cut(s) 161
SfuI TTCGAA 2 cut(s) 629, 2014
SgrBI CCGCGG 1 cut(s) 161
SinI GGWCC 4 cut(s) 288, 1865, 2039, 2047
SmiMI CAYNNNNRTG 3 cut(s) 852, 1188, 1683
SmlI CTYRAG 2 cut(s) 1869, 1997
SmoI CTYRAG 2 cut(s) 1869, 1997
Sse9I AATT 8 cut(s) 814, 1367, 1380, 1596, 1621, 1856, 2017, 2030
SspI AATATT 2 cut(s) 703, 1414
SspMI CTAG 3 cut(s) 576, 711, 1986
StyD4I CCNGG 8 cut(s) 170, 201, 277, 395, 917, 1295, 1480, 1899
TaaI ACNGT 4 cut(s) 125, 772, 1766, 1980
TaiI ACGT 1 cut(s) 1503
TasI AATT 8 cut(s) 814, 1367, 1380, 1596, 1621, 1856, 2017, 2030
TatI WGTACW 1 cut(s) 1814
TauI GCSGC 3 cut(s) 163, 166, 1257
TfiI GAWTC 3 cut(s) 344, 368, 1529
Tru1I TTAA 7 cut(s) 687, 813, 1383, 1491, 1650, 2055, 2062
Tru9I TTAA 7 cut(s) 687, 813, 1383, 1491, 1650, 2055, 2062
TscAI CASTG 6 cut(s) 130, 308, 501, 621, 1141, 1771
TseFI GTSAC 1 cut(s) 418
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 6 cut(s) 870, 1232, 1328, 1466, 1934, 2010
TspGWI ACGGA 2 cut(s) 1836, 1995
TspRI CASTG 6 cut(s) 130, 308, 501, 621, 1141, 1771
VpaK11BI GGWCC 4 cut(s) 288, 1865, 2039, 2047
VspI ATTAAT 1 cut(s) 2055
XapI RAATTY 3 cut(s) 1621, 1856, 2017
XcmI CCANNNNNNNNNTGG 2 cut(s) 1511, 1562
XmnI GAANNNNTTC 1 cut(s) 1473
XspI CTAG 3 cut(s) 576, 711, 1986
Zsp2I ATGCAT 2 cut(s) 1090, 1639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.