Rorug06G0292800

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001271
Physical Location & Seq
Forward (+)
44477229 .. 44478940
1712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0292800.1

Sequence Viewer

Length: 1092 bp
ATGTTGCTGAGGAGCTCTTCATCACCGGTTATTGGATCCCTTCTTGCATCATTCTCAGCTGAAAGTCCACTTGGCAATCACTTTGAACCAAAACCAACCTCAATCCACCACACTCCCACCAAGTTTTCATTTCACCAAACTGGGCCTCTCAACATCTCACACGGTTCCTCACCAATCTCTCCCTCCATTGATCTTTCCTCCCAGTTTCTTCGGAGGGCTCAGTCTGATGGAAACTTGGAAGGACTGGCCTATGCAGCTTCTTGTGATAAACATGAAGATGATCCGTTCTTCTGTGATTTATACCAACCCAACAAGTCGAGATCCAGGGGAACACCGTTGCAAACTATAGATTCTTTTTCGACCTGTAACTCGGGAGCTTTGGGTGAAGATGAAGAAGATAGTGATAGGGAATTTGATGAAGAGGTTGAGGAAAGTGAGAGAGTCATGGCTATTGAAGGGGATAGTGTTGGTTTGGAAGAGAAGGGGAGTCTATCCGAGGACATGAGAACTATGCAGAAAATGTGGGGTGGGGGTTATGAAGAACAAAGAGAGCTAGAAAGTCGAGAAATGCATCTTGCAAAGGGACTTGGGATTGGTGGTGGAAGTGGCGGTGGCCGGGGTGGTCACGAGGCTAATTGGGAAAGCACTGGTGGAGATGGAGATGGAGATGGAGATGGAGATGACAACAACCAGGGGGTTGAGGAGCATTACAAGAAGATGGTAGTCGAACATCCTGGAAACCCATTGGTTCTCGGAAATTATGCTAATTTTTTGTATCAGACCAAGAGAGACCTTCCTTTAGCAGAGGAATACTACTCTCGTTCGATCCTAGCAGACCCGAATGACGGTAAAATTTTGGCACAATATGCCAAGGTACTATGGGAGCTGCACCATGATGAAGATAGAGCCTTAAGCTACTATGAACGAGCAGTCCAAGCTGCACCTCAAGATAGCCATGTCCATGCAGCATACGCTGGCTTCCTATGGGAAACAGAGGAAGATGAAGATGAAGATGAAGCAGATCTGGGACAAGATCTTGGCGGCATGTCGCCATATATTCATGAAGGATTCATGACTTCTGCAAGCGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

363

Amino Acids

39.76

Weight (kDa)

4.47

Isoelectric Point (pI)

65.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 609, 1041
AclWI GGATC 5 cut(s) 30, 43, 275, 315, 820
AcoI YGGCCR 1 cut(s) 613
AcsI RAATTY 2 cut(s) 410, 852
AfaI GTAC 1 cut(s) 876
AfiI CCNNNNNNNGG 2 cut(s) 32, 845
AflII CTTAAG 1 cut(s) 910
AgeI ACCGGT 1 cut(s) 25
AgsI TTSAA 2 cut(s) 86, 455
AjnI CCWGG 3 cut(s) 323, 690, 733
AjuI GAANNNNNNNTTGG 2 cut(s) 54, 86
AloI GAACNNNNNNTCC 2 cut(s) 915, 947
AluBI AGCT 8 cut(s) 15, 59, 257, 377, 553, 886, 915, 938
AluI AGCT 8 cut(s) 15, 59, 257, 377, 553, 886, 915, 938
Alw21I GWGCWC 1 cut(s) 17
Alw26I GTCTC 1 cut(s) 783
AlwI GGATC 5 cut(s) 30, 43, 275, 315, 820
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 3 cut(s) 143, 246, 613
ApeKI GCWGC 4 cut(s) 254, 886, 938, 965
ApoI RAATTY 2 cut(s) 410, 852
AsiGI ACCGGT 1 cut(s) 25
AspLEI GCGC 1 cut(s) 1088
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 617
AsuHPI GGTGA 4 cut(s) 15, 125, 162, 395
AvaI CYCGRG 1 cut(s) 370
BamHI GGATCC 1 cut(s) 35
BanII GRGCYC 2 cut(s) 17, 220
BauI CACGAG 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 17
BbvCI CCTCAGC 1 cut(s) 8
BbvI GCAGC 4 cut(s) 266, 873, 925, 977
BccI CCATC 6 cut(s) 221, 650, 656, 662, 668, 712
BciT130I CCWGG 3 cut(s) 325, 692, 735
BcnI CCSGG 1 cut(s) 617
BcoDI GTCTC 1 cut(s) 783
BfaI CTAG 2 cut(s) 554, 830
BfmI CTRYAG 1 cut(s) 345
BfoI RGCGCY 1 cut(s) 1089
BfrI CTTAAG 1 cut(s) 910
BglII AGATCT 2 cut(s) 1021, 1033
BisI GCNGC 5 cut(s) 255, 887, 939, 966, 1042
BlsI GCNGC 5 cut(s) 256, 888, 940, 967, 1043
Bme1390I CCNGG 4 cut(s) 325, 617, 692, 735
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 2 cut(s) 37, 166
BmrFI CCNGG 4 cut(s) 325, 617, 692, 735
BmrI ACTGGG 2 cut(s) 150, 196
BmsI GCATC 2 cut(s) 56, 580
BmuI ACTGGG 2 cut(s) 150, 196
Bpu10I CCTNAGC 1 cut(s) 8
BpuEI CTTGAG 1 cut(s) 930
BpuMI CCSGG 1 cut(s) 617
BsaI GGTCTC 1 cut(s) 783
BsaJI CCNNGG 5 cut(s) 324, 495, 616, 691, 870
BsaWI WCCGGW 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 845
Bse118I RCCGGY 1 cut(s) 25
Bse1I ACTGG 4 cut(s) 145, 202, 249, 652
BseBI CCWGG 3 cut(s) 325, 692, 735
BseDI CCNNGG 5 cut(s) 324, 495, 616, 691, 870
BseGI GGATG 1 cut(s) 730
BseLI CCNNNNNNNGG 2 cut(s) 32, 845
BseMII CTCAG 2 cut(s) 69, 233
BseNI ACTGG 4 cut(s) 145, 202, 249, 652
BseRI GAGGAG 2 cut(s) 25, 716
BseXI GCAGC 4 cut(s) 266, 873, 925, 977
BsgI GTGCAG 2 cut(s) 872, 924
BshFI GGCC 3 cut(s) 145, 248, 615
BshTI ACCGGT 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 370
BsiSI CCGG 2 cut(s) 26, 616
BslFI GGGAC 2 cut(s) 597, 1041
BslI CCNNNNNNNGG 2 cut(s) 32, 845
BsmAI GTCTC 1 cut(s) 783
BsmFI GGGAC 2 cut(s) 597, 1041
BsnI GGCC 3 cut(s) 145, 248, 615
Bso31I GGTCTC 1 cut(s) 783
BsoBI CYCGRG 1 cut(s) 370
Bsp1286I GDGCHC 2 cut(s) 17, 220
Bsp143I GATC 7 cut(s) 35, 190, 280, 320, 825, 1021, 1033
BspACI CCGC 2 cut(s) 609, 1041
BspANI GGCC 3 cut(s) 145, 248, 615
BspCNI CTCAG 2 cut(s) 68, 232
BspHI TCATGA 2 cut(s) 1060, 1071
BspLI GGNNCC 2 cut(s) 37, 166
BspPI GGATC 5 cut(s) 30, 43, 275, 315, 820
BspQI GCTCTTC 1 cut(s) 22
BspTI CTTAAG 1 cut(s) 910
BspTNI GGTCTC 1 cut(s) 783
BsrFI RCCGGY 1 cut(s) 25
BsrI ACTGG 4 cut(s) 145, 202, 249, 652
BssAI RCCGGY 1 cut(s) 25
BssECI CCNNGG 5 cut(s) 324, 495, 616, 691, 870
BssMI GATC 7 cut(s) 35, 190, 280, 320, 825, 1021, 1033
BssSI CACGAG 1 cut(s) 626
BssT1I CCWWGG 1 cut(s) 870
Bst2BI CACGAG 1 cut(s) 626
Bst2UI CCWGG 3 cut(s) 325, 692, 735
Bst4CI ACNGT 3 cut(s) 164, 336, 848
Bst6I CTCTTC 3 cut(s) 22, 414, 471
BstAFI CTTAAG 1 cut(s) 910
BstAPI GCANNNNNTGC 1 cut(s) 866
BstC8I GCNNGC 2 cut(s) 976, 1084
BstDEI CTNAG 3 cut(s) 8, 55, 219
BstF5I GGATG 1 cut(s) 730
BstH2I RGCGCY 1 cut(s) 1089
BstHHI GCGC 1 cut(s) 1088
BstKTI GATC 7 cut(s) 38, 193, 283, 323, 828, 1024, 1036
BstMAI GTCTC 1 cut(s) 783
BstMBI GATC 7 cut(s) 35, 190, 280, 320, 825, 1021, 1033
BstMWI GCNNNNNNNGC 4 cut(s) 254, 866, 935, 971
BstNI CCWGG 3 cut(s) 325, 692, 735
BstNSI RCATGY 1 cut(s) 1048
BstSCI CCNGG 4 cut(s) 323, 615, 690, 733
BstSFI CTRYAG 1 cut(s) 345
BstV1I GCAGC 4 cut(s) 266, 873, 925, 977
BstX2I RGATCY 4 cut(s) 35, 320, 1021, 1033
BstYI RGATCY 4 cut(s) 35, 320, 1021, 1033
BsuRI GGCC 3 cut(s) 145, 248, 615
BtsCI GGATG 1 cut(s) 730
BtsIMutI CAGTG 1 cut(s) 645
Cac8I GCNNGC 2 cut(s) 976, 1084
CciI TCATGA 2 cut(s) 1060, 1071
CfoI GCGC 1 cut(s) 1088
Cfr10I RCCGGY 1 cut(s) 25
Cfr13I GGNCC 1 cut(s) 143
Csp6I GTAC 1 cut(s) 875
CspAI ACCGGT 1 cut(s) 25
CviAII CATG 9 cut(s) 272, 445, 502, 893, 956, 962, 1045, 1061, 1072
CviQI GTAC 1 cut(s) 875
DdeI CTNAG 3 cut(s) 8, 55, 219
DpnI GATC 7 cut(s) 37, 192, 282, 322, 827, 1023, 1035
DpnII GATC 7 cut(s) 35, 190, 280, 320, 825, 1021, 1033
EaeI YGGCCR 1 cut(s) 613
Eam1104I CTCTTC 3 cut(s) 22, 414, 471
EarI CTCTTC 3 cut(s) 22, 414, 471
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 870
Eco24I GRGCYC 2 cut(s) 17, 220
Eco31I GGTCTC 1 cut(s) 783
Eco53kI GAGCTC 1 cut(s) 15
Eco88I CYCGRG 1 cut(s) 370
EcoICRI GAGCTC 1 cut(s) 15
EcoRII CCWGG 3 cut(s) 323, 690, 733
EcoT14I CCWWGG 1 cut(s) 870
EcoT22I ATGCAT 1 cut(s) 573
EcoT38I GRGCYC 2 cut(s) 17, 220
ErhI CCWWGG 1 cut(s) 870
FaeI CATG 9 cut(s) 275, 448, 505, 896, 959, 965, 1048, 1064, 1075
FaqI GGGAC 2 cut(s) 597, 1041
FatI CATG 9 cut(s) 271, 444, 501, 892, 955, 961, 1044, 1060, 1071
Fnu4HI GCNGC 5 cut(s) 255, 887, 939, 966, 1042
FokI GGATG 1 cut(s) 717
FriOI GRGCYC 2 cut(s) 17, 220
Fsp4HI GCNGC 5 cut(s) 255, 887, 939, 966, 1042
FspBI CTAG 2 cut(s) 554, 830
GlaI GCGC 1 cut(s) 1087
GluI GCNGC 5 cut(s) 255, 887, 939, 966, 1042
HaeII RGCGCY 1 cut(s) 1089
HaeIII GGCC 3 cut(s) 145, 248, 615
HapII CCGG 2 cut(s) 26, 616
HhaI GCGC 1 cut(s) 1088
Hin1II CATG 9 cut(s) 275, 448, 505, 896, 959, 965, 1048, 1064, 1075
Hin6I GCGC 1 cut(s) 1086
HinP1I GCGC 1 cut(s) 1086
HinfI GANTC 4 cut(s) 350, 441, 487, 1068
HpaII CCGG 2 cut(s) 26, 616
HphI GGTGA 4 cut(s) 15, 125, 162, 395
Hpy166II GTNNAC 1 cut(s) 68
Hpy188I TCNGA 5 cut(s) 213, 226, 496, 755, 780
Hpy188III TCNNGA 7 cut(s) 318, 372, 563, 626, 947, 1061, 1072
Hpy8I GTNNAC 1 cut(s) 68
HpyAV CCTTC 6 cut(s) 50, 233, 449, 475, 803, 1058
HpyCH4III ACNGT 3 cut(s) 164, 336, 848
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 866, 935, 971
HpyF3I CTNAG 3 cut(s) 8, 55, 219
Hsp92II CATG 9 cut(s) 275, 448, 505, 896, 959, 965, 1048, 1064, 1075
HspAI GCGC 1 cut(s) 1086
Kzo9I GATC 7 cut(s) 35, 190, 280, 320, 825, 1021, 1033
LguI GCTCTTC 1 cut(s) 22
LmnI GCTCC 4 cut(s) 12, 374, 703, 883
Lsp1109I GCAGC 4 cut(s) 266, 873, 925, 977
LweI GCATC 2 cut(s) 56, 580
MaeI CTAG 2 cut(s) 554, 830
MaeIII GTNAC 2 cut(s) 365, 623
MalI GATC 7 cut(s) 37, 192, 282, 322, 827, 1023, 1035
MboI GATC 7 cut(s) 35, 190, 280, 320, 825, 1021, 1033
MflI RGATCY 4 cut(s) 35, 320, 1021, 1033
MhlI GDGCHC 2 cut(s) 17, 220
MluCI AATT 5 cut(s) 410, 634, 757, 766, 852
MlyI GAGTC 2 cut(s) 450, 496
Mph1103I ATGCAT 1 cut(s) 573
MseI TTAA 1 cut(s) 911
MslI CAYNNNNRTG 3 cut(s) 276, 894, 960
MspA1I CMGCKG 1 cut(s) 59
MspCI CTTAAG 1 cut(s) 910
MspI CCGG 2 cut(s) 26, 616
MspR9I CCNGG 4 cut(s) 325, 617, 692, 735
MvaI CCWGG 3 cut(s) 325, 692, 735
MwoI GCNNNNNNNGC 4 cut(s) 254, 866, 935, 971
NciI CCSGG 1 cut(s) 617
NdeII GATC 7 cut(s) 35, 190, 280, 320, 825, 1021, 1033
NlaIII CATG 9 cut(s) 275, 448, 505, 896, 959, 965, 1048, 1064, 1075
NlaIV GGNNCC 2 cut(s) 37, 166
NmuCI GTSAC 1 cut(s) 623
NsiI ATGCAT 1 cut(s) 573
NspI RCATGY 1 cut(s) 1048
PagI TCATGA 2 cut(s) 1060, 1071
PciSI GCTCTTC 1 cut(s) 22
PfeI GAWTC 2 cut(s) 350, 1068
PfoI TCCNGGA 1 cut(s) 733
PinAI ACCGGT 1 cut(s) 25
PkrI GCNGC 5 cut(s) 256, 888, 940, 967, 1043
PleI GAGTC 2 cut(s) 449, 495
PpsI GAGTC 2 cut(s) 449, 495
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 3 cut(s) 323, 690, 733
PspGI CCWGG 3 cut(s) 323, 690, 733
PspN4I GGNNCC 2 cut(s) 37, 166
PspPI GGNCC 1 cut(s) 143
PsuI RGATCY 4 cut(s) 35, 320, 1021, 1033
PvuII CAGCTG 1 cut(s) 59
RsaI GTAC 1 cut(s) 876
RsaNI GTAC 1 cut(s) 875
RseI CAYNNNNRTG 3 cut(s) 276, 894, 960
SacI GAGCTC 1 cut(s) 17
SapI GCTCTTC 1 cut(s) 22
SaqAI TTAA 1 cut(s) 911
SatI GCNGC 5 cut(s) 255, 887, 939, 966, 1042
Sau3AI GATC 7 cut(s) 35, 190, 280, 320, 825, 1021, 1033
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 2 cut(s) 450, 496
ScrFI CCNGG 4 cut(s) 325, 617, 692, 735
SduI GDGCHC 2 cut(s) 17, 220
SfaNI GCATC 2 cut(s) 56, 580
SfcI CTRYAG 1 cut(s) 345
SmiMI CAYNNNNRTG 3 cut(s) 276, 894, 960
SmlI CTYRAG 2 cut(s) 910, 945
SmoI CTYRAG 2 cut(s) 910, 945
Sse9I AATT 5 cut(s) 410, 634, 757, 766, 852
SsiI CCGC 2 cut(s) 609, 1041
SspMI CTAG 2 cut(s) 554, 830
SstI GAGCTC 1 cut(s) 17
StyD4I CCNGG 4 cut(s) 323, 615, 690, 733
StyI CCWWGG 1 cut(s) 870
TaaI ACNGT 3 cut(s) 164, 336, 848
TaqI TCGA 5 cut(s) 317, 359, 562, 726, 824
TasI AATT 5 cut(s) 410, 634, 757, 766, 852
TauI GCSGC 1 cut(s) 1044
TfiI GAWTC 2 cut(s) 350, 1068
Tru1I TTAA 1 cut(s) 911
Tru9I TTAA 1 cut(s) 911
TscAI CASTG 1 cut(s) 652
TseFI GTSAC 1 cut(s) 623
TseI GCWGC 4 cut(s) 254, 886, 938, 965
Tsp45I GTSAC 1 cut(s) 623
TspGWI ACGGA 1 cut(s) 273
TspRI CASTG 1 cut(s) 652
Vha464I CTTAAG 1 cut(s) 910
XapI RAATTY 2 cut(s) 410, 852
XceI RCATGY 1 cut(s) 1048
XspI CTAG 2 cut(s) 554, 830
Zsp2I ATGCAT 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.