Prupe.1G389700_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001271
Physical Location & Seq
Forward (+)
34793951 .. 34796289
2339 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G389700.1

Sequence Viewer

Length: 1167 bp
ATGGATCCTTTTCCTACTTTTGGGGTTTCTCGAGAACAATATCAGGATTCTTATCCTTCAAGATTACAACCAAACAATCCTCATCATAGTCCCAGTCATATTTCTCCTCCTCACAGCCCCACATCTCGAAGTCTACGCTTCAACCACGAAAGTTCAAATGGAAATGCATTTGCGTTGCATCATAAATCATTTACAGAAAGGGCTGAAAGTAGTAGTCAAGTGACAGTCAATGCTCATCCTTTGCCTTTGCCTCCTGGAGCAGTGGAACCACCACAGTCATCTATCATGCATCATAATGCAGAACTTGTATCCACATCATCAATGAAACCTCAATGGCAGAAGGGAAAACTTATTGGACGCGGTACCTTTGGTAGGGTTTATCATGCAACCAACCGAGATACCGGCGCCTTGTGTGCAATGAAAGAAGTTGATCTCATTCCCGATGATCCTAAATTTGCTGAATGTATAAAGCAATTGGAGCAGGAAATTGAAGTCCTCCGTACACTGAATCATCCGAATATTGTGCAGTATTATGGTAGTGAAGTAATTGATGATCACTTCTACATGTACTTGGAGTACGTTTATCCTGGATCAATCAATAAGTACGTCCAAGACCATATTATTGGAGCCATGACAGAATCTGTAGTTCGCAATTTTACTCGCCATATCCTCTATGGGCTGGCCTTTTTGCATAGCAAGAAGATAGTTCACAGGGACATAAAGGGTGCAAATTTGTGCGTTGATACATCAGGAGTTGTTAAAATTGCTGGTTTTGGGATGGCAAAACGACTTAACGGACAGTCAAACAATCTTTCTCTGAAGGGCAGTCCATACTGGATGGCTCCTGAGGTCGTAATACAAAACAATCCAGATCTTGGTTTGGCCGTTGATATATGGAGTTTGGGTTGTACCATCATTGAGATGTTGAATGGAAAACCTCCTTGGAGTGACTTTACAGGACCTCAAGCTATGTTCAAGGTTCTGGAAAAAATCCCAGATATACCAGAAACATTATCTGCAGAAGGAAAGGACTTCCTCAGTCGGTGTTTTCGTAGGAACCCTGCAGAGAGACCATCAGCTAATCAGCTACTTGAGCATCCTTTTGTACTAAACTCACATGATCAAACTATCTCACACTATGCCCACAATTTTTCTCTGATGAACCTG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

389

Amino Acids

43.67

Weight (kDa)

6.72

Isoelectric Point (pI)

51.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 2 cut(s) 362, 404
AccB7I CCANNNNNTGG 1 cut(s) 875
AccI GTMKAC 1 cut(s) 133
AccII CGCG 1 cut(s) 360
AciI CCGC 1 cut(s) 360
AclWI GGATC 3 cut(s) 12, 440, 598
AcoI YGGCCR 1 cut(s) 882
AcsI RAATTY 2 cut(s) 452, 730
AcuI CTGAAG 1 cut(s) 839
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 7 cut(s) 364, 502, 569, 578, 605, 910, 1107
AfiI CCNNNNNNNGG 3 cut(s) 20, 372, 875
AflIII ACRYGT 1 cut(s) 564
AgsI TTSAA 6 cut(s) 60, 142, 156, 491, 928, 976
AjnI CCWGG 2 cut(s) 253, 586
AjuI GAANNNNNNNTTGG 2 cut(s) 925, 957
AluBI AGCT 3 cut(s) 968, 1079, 1087
AluI AGCT 3 cut(s) 968, 1079, 1087
Alw26I GTCTC 1 cut(s) 1063
AlwI GGATC 3 cut(s) 12, 440, 598
AlwNI CAGNNNCTG 1 cut(s) 641
Ama87I CYCGRG 1 cut(s) 30
AoxI GGCC 2 cut(s) 681, 882
ApoI RAATTY 2 cut(s) 452, 730
Asp718I GGTACC 1 cut(s) 362
AspLEI GCGC 1 cut(s) 407
AspS9I GGNCC 1 cut(s) 959
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 1 cut(s) 959
AxyI CCTNAGG 1 cut(s) 846
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 2 cut(s) 362, 404
BccI CCATC 4 cut(s) 772, 832, 920, 1081
BceAI ACGGC 1 cut(s) 869
BcgI CGANNNNNNTGC 2 cut(s) 505, 539
BciT130I CCWGG 2 cut(s) 255, 588
BciVI GTATCC 1 cut(s) 319
BclI TGATCA 2 cut(s) 553, 1120
BcoDI GTCTC 1 cut(s) 1063
BfmI CTRYAG 3 cut(s) 642, 1017, 1062
BfoI RGCGCY 1 cut(s) 408
BfuI GTATCC 1 cut(s) 319
BglII AGATCT 1 cut(s) 871
Bme1390I CCNGG 2 cut(s) 255, 588
Bme18I GGWCC 1 cut(s) 959
BmeT110I CYCGRG 1 cut(s) 30
BmgT120I GGNCC 1 cut(s) 959
BmiI GGNNCC 7 cut(s) 6, 267, 364, 406, 628, 843, 1058
BmrFI CCNGG 2 cut(s) 255, 588
BmrI ACTGGG 1 cut(s) 87
BmsI GCATC 3 cut(s) 187, 298, 1105
BmuI ACTGGG 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 276
BpuEI CTTGAG 2 cut(s) 948, 1112
BsaBI GATNNNNATC 1 cut(s) 51
BsaHI GRCGYC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 1063
BsaJI CCNNGG 1 cut(s) 941
BsaXI ACNNNNNCTCC 2 cut(s) 889, 919
Bsc4I CCNNNNNNNGG 3 cut(s) 20, 372, 875
Bse118I RCCGGY 1 cut(s) 401
Bse1I ACTGG 2 cut(s) 93, 839
Bse21I CCTNAGG 1 cut(s) 846
Bse3DI GCAATG 1 cut(s) 423
Bse8I GATNNNNATC 1 cut(s) 51
BseBI CCWGG 2 cut(s) 255, 588
BseDI CCNNGG 1 cut(s) 941
BseGI GGATG 5 cut(s) 235, 511, 783, 843, 1096
BseJI GATNNNNATC 1 cut(s) 51
BseLI CCNNNNNNNGG 3 cut(s) 20, 372, 875
BseMI GCAATG 1 cut(s) 423
BseMII CTCAG 2 cut(s) 837, 1051
BseNI ACTGG 2 cut(s) 93, 839
BseRI GAGGAG 2 cut(s) 96, 99
BsgI GTGCAG 1 cut(s) 545
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 2 cut(s) 683, 884
BshNI GGYRCC 2 cut(s) 362, 404
BsiHKCI CYCGRG 1 cut(s) 30
BsiSI CCGG 1 cut(s) 402
BslFI GGGAC 2 cut(s) 75, 728
BslI CCNNNNNNNGG 3 cut(s) 20, 372, 875
BsmAI GTCTC 1 cut(s) 1063
BsmFI GGGAC 2 cut(s) 75, 728
BsnI GGCC 2 cut(s) 683, 884
Bso31I GGTCTC 1 cut(s) 1063
BsoBI CYCGRG 1 cut(s) 30
Bsp143I GATC 7 cut(s) 4, 430, 445, 553, 590, 871, 1120
BspACI CCGC 1 cut(s) 360
BspANI GGCC 2 cut(s) 683, 884
BspCNI CTCAG 2 cut(s) 838, 1050
BspFNI CGCG 1 cut(s) 360
BspLI GGNNCC 7 cut(s) 6, 267, 364, 406, 628, 843, 1058
BspMAI CTGCAG 2 cut(s) 1021, 1066
BspPI GGATC 3 cut(s) 12, 440, 598
BspT107I GGYRCC 2 cut(s) 362, 404
BspTNI GGTCTC 1 cut(s) 1063
BsrDI GCAATG 1 cut(s) 423
BsrFI RCCGGY 1 cut(s) 401
BsrI ACTGG 2 cut(s) 93, 839
BssAI RCCGGY 1 cut(s) 401
BssECI CCNNGG 1 cut(s) 941
BssMI GATC 7 cut(s) 4, 430, 445, 553, 590, 871, 1120
BssNI GRCGYC 1 cut(s) 405
BssT1I CCWWGG 1 cut(s) 941
Bst2UI CCWGG 2 cut(s) 255, 588
Bst4CI ACNGT 3 cut(s) 226, 276, 801
BstACI GRCGYC 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 681
BstDEI CTNAG 2 cut(s) 846, 1037
BstENI CCTNNNNNAGG 1 cut(s) 370
BstF5I GGATG 5 cut(s) 235, 511, 783, 843, 1096
BstFNI CGCG 1 cut(s) 360
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 1 cut(s) 407
BstKTI GATC 7 cut(s) 7, 433, 448, 556, 593, 874, 1123
BstMAI GTCTC 1 cut(s) 1063
BstMBI GATC 7 cut(s) 4, 430, 445, 553, 590, 871, 1120
BstMWI GCNNNNNNNGC 3 cut(s) 413, 478, 1093
BstNI CCWGG 2 cut(s) 255, 588
BstNSI RCATGY 1 cut(s) 568
BstSCI CCNGG 2 cut(s) 253, 586
BstSFI CTRYAG 3 cut(s) 642, 1017, 1062
BstUI CGCG 1 cut(s) 360
BstX2I RGATCY 2 cut(s) 4, 871
BstXI CCANNNNNNTGG 1 cut(s) 623
BstYI RGATCY 2 cut(s) 4, 871
Bsu36I CCTNAGG 1 cut(s) 846
BsuI GTATCC 1 cut(s) 319
BsuRI GGCC 2 cut(s) 683, 884
BtsCI GGATG 5 cut(s) 235, 511, 783, 843, 1096
BtsI GCAGTG 1 cut(s) 267
BtsIMutI CAGTG 2 cut(s) 267, 503
Cac8I GCNNGC 1 cut(s) 681
CaiI CAGNNNCTG 1 cut(s) 641
CfoI GCGC 1 cut(s) 407
Cfr10I RCCGGY 1 cut(s) 401
Cfr13I GGNCC 1 cut(s) 959
CseI GACGC 1 cut(s) 366
Csp6I GTAC 7 cut(s) 363, 501, 568, 577, 604, 909, 1106
CviAII CATG 5 cut(s) 286, 383, 565, 631, 1118
CviQI GTAC 7 cut(s) 363, 501, 568, 577, 604, 909, 1106
DdeI CTNAG 2 cut(s) 846, 1037
DinI GGCGCC 1 cut(s) 406
DpnI GATC 7 cut(s) 6, 432, 447, 555, 592, 873, 1122
DpnII GATC 7 cut(s) 4, 430, 445, 553, 590, 871, 1120
EaeI YGGCCR 1 cut(s) 882
Eco130I CCWWGG 1 cut(s) 941
Eco31I GGTCTC 1 cut(s) 1063
Eco47I GGWCC 1 cut(s) 959
Eco57I CTGAAG 1 cut(s) 839
Eco81I CCTNAGG 1 cut(s) 846
Eco88I CYCGRG 1 cut(s) 30
EcoNI CCTNNNNNAGG 1 cut(s) 370
EcoO109I RGGNCCY 1 cut(s) 959
EcoRII CCWGG 2 cut(s) 253, 586
EcoT14I CCWWGG 1 cut(s) 941
EcoT22I ATGCAT 2 cut(s) 169, 291
EgeI GGCGCC 1 cut(s) 406
EheI GGCGCC 1 cut(s) 406
ErhI CCWWGG 1 cut(s) 941
FaeI CATG 5 cut(s) 289, 386, 568, 634, 1121
FaqI GGGAC 2 cut(s) 75, 728
FatI CATG 5 cut(s) 285, 382, 564, 630, 1117
FbaI TGATCA 2 cut(s) 553, 1120
FblI GTMKAC 1 cut(s) 133
FokI GGATG 5 cut(s) 222, 498, 790, 850, 1083
GlaI GCGC 1 cut(s) 406
GsuI CTGGAG 1 cut(s) 276
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 2 cut(s) 683, 884
HapII CCGG 1 cut(s) 402
HgaI GACGC 1 cut(s) 366
HhaI GCGC 1 cut(s) 407
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 5 cut(s) 289, 386, 568, 634, 1121
Hin6I GCGC 1 cut(s) 405
HinP1I GCGC 1 cut(s) 405
HinfI GANTC 3 cut(s) 47, 508, 638
HpaII CCGG 1 cut(s) 402
Hpy166II GTNNAC 3 cut(s) 134, 503, 709
Hpy188I TCNGA 3 cut(s) 516, 819, 1158
Hpy8I GTNNAC 3 cut(s) 134, 503, 709
HpyAV CCTTC 4 cut(s) 66, 334, 814, 1016
HpyCH4III ACNGT 3 cut(s) 226, 276, 801
HpyCH4IV ACGT 2 cut(s) 579, 606
HpyF10VI GCNNNNNNNGC 3 cut(s) 413, 478, 1093
HpyF3I CTNAG 2 cut(s) 846, 1037
HpySE526I ACGT 2 cut(s) 579, 606
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 5 cut(s) 289, 386, 568, 634, 1121
HspAI GCGC 1 cut(s) 405
KasI GGCGCC 1 cut(s) 404
KpnI GGTACC 1 cut(s) 366
Ksp22I TGATCA 2 cut(s) 553, 1120
Kzo9I GATC 7 cut(s) 4, 430, 445, 553, 590, 871, 1120
LmnI GCTCC 4 cut(s) 257, 478, 626, 847
LweI GCATC 3 cut(s) 187, 298, 1105
MaeII ACGT 2 cut(s) 579, 606
MaeIII GTNAC 2 cut(s) 220, 947
MalI GATC 7 cut(s) 6, 432, 447, 555, 592, 873, 1122
MboI GATC 7 cut(s) 4, 430, 445, 553, 590, 871, 1120
MboII GAAGA 1 cut(s) 712
MfeI CAATTG 1 cut(s) 473
MflI RGATCY 2 cut(s) 4, 871
MluCI AATT 8 cut(s) 452, 473, 486, 546, 652, 730, 762, 1147
Mly113I GGCGCC 1 cut(s) 405
Mph1103I ATGCAT 2 cut(s) 169, 291
MseI TTAA 2 cut(s) 759, 792
MslI CAYNNNNRTG 2 cut(s) 294, 920
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 2 cut(s) 255, 588
MunI CAATTG 1 cut(s) 473
MvaI CCWGG 2 cut(s) 255, 588
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 3 cut(s) 413, 478, 1093
NarI GGCGCC 1 cut(s) 405
NdeII GATC 7 cut(s) 4, 430, 445, 553, 590, 871, 1120
NlaIII CATG 5 cut(s) 289, 386, 568, 634, 1121
NlaIV GGNNCC 7 cut(s) 6, 267, 364, 406, 628, 843, 1058
NmuCI GTSAC 2 cut(s) 220, 947
NsiI ATGCAT 2 cut(s) 169, 291
NspI RCATGY 1 cut(s) 568
PaeR7I CTCGAG 1 cut(s) 30
PciI ACATGT 1 cut(s) 564
PcsI WCGNNNNNNNCGW 1 cut(s) 1048
PfeI GAWTC 3 cut(s) 47, 508, 638
PflMI CCANNNNNTGG 1 cut(s) 875
PfoI TCCNGGA 2 cut(s) 253, 586
PluTI GGCGCC 1 cut(s) 408
PpuMI RGGWCCY 1 cut(s) 959
PscI ACATGT 1 cut(s) 564
Psp5II RGGWCCY 1 cut(s) 959
Psp6I CCWGG 2 cut(s) 253, 586
PspGI CCWGG 2 cut(s) 253, 586
PspN4I GGNNCC 7 cut(s) 6, 267, 364, 406, 628, 843, 1058
PspPI GGNCC 1 cut(s) 959
PspPPI RGGWCCY 1 cut(s) 959
PstI CTGCAG 2 cut(s) 1021, 1066
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 2 cut(s) 4, 871
RsaI GTAC 7 cut(s) 364, 502, 569, 578, 605, 910, 1107
RsaNI GTAC 7 cut(s) 363, 501, 568, 577, 604, 909, 1106
RseI CAYNNNNRTG 2 cut(s) 294, 920
SaqAI TTAA 2 cut(s) 759, 792
Sau3AI GATC 7 cut(s) 4, 430, 445, 553, 590, 871, 1120
Sau96I GGNCC 1 cut(s) 959
ScrFI CCNGG 2 cut(s) 255, 588
SfaNI GCATC 3 cut(s) 187, 298, 1105
SfcI CTRYAG 3 cut(s) 642, 1017, 1062
SfoI GGCGCC 1 cut(s) 406
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 1 cut(s) 959
SlaI CTCGAG 1 cut(s) 30
SmiMI CAYNNNNRTG 2 cut(s) 294, 920
SmlI CTYRAG 3 cut(s) 30, 963, 1091
SmoI CTYRAG 3 cut(s) 30, 963, 1091
Sse9I AATT 8 cut(s) 452, 473, 486, 546, 652, 730, 762, 1147
SsiI CCGC 1 cut(s) 360
SspDI GGCGCC 1 cut(s) 404
SspI AATATT 1 cut(s) 520
StyD4I CCNGG 2 cut(s) 253, 586
StyI CCWWGG 1 cut(s) 941
TaaI ACNGT 3 cut(s) 226, 276, 801
TaiI ACGT 2 cut(s) 582, 609
TaqI TCGA 2 cut(s) 31, 127
TasI AATT 8 cut(s) 452, 473, 486, 546, 652, 730, 762, 1147
TatI WGTACW 2 cut(s) 567, 1105
TfiI GAWTC 3 cut(s) 47, 508, 638
Tru1I TTAA 2 cut(s) 759, 792
Tru9I TTAA 2 cut(s) 759, 792
TscAI CASTG 2 cut(s) 267, 510
TseFI GTSAC 2 cut(s) 220, 947
Tsp45I GTSAC 2 cut(s) 220, 947
TspDTI ATGAA 2 cut(s) 338, 434
TspGWI ACGGA 2 cut(s) 488, 810
TspRI CASTG 2 cut(s) 267, 510
Van91I CCANNNNNTGG 1 cut(s) 875
VpaK11BI GGWCC 1 cut(s) 959
XagI CCTNNNNNAGG 1 cut(s) 370
XapI RAATTY 2 cut(s) 452, 730
XceI RCATGY 1 cut(s) 568
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 1 cut(s) 133
Zsp2I ATGCAT 2 cut(s) 169, 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.