Rorug02G0123200

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001271
Physical Location & Seq
Reverse (-)
10590123 .. 10591750
1628 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0123200.1

Sequence Viewer

Length: 1215 bp
ATGGCGTCGAGCTACAGACTGAGAGCCTCCACGTCTGCTTCATCATCATCATCTGCTCGGTGGAAATACGACGTGTTCCTGAGTTTCAGGGGTGAAGATACCCGCAAGAAGTTCACGGACCATTTATACGACAAATTGCAGTGGCGGGGAATCACAACTTTCAGAGACGACCCAAATCTTCAGAGAGGTACAACTATCTCTCCAGATCTCCTGGCTGCAATTGAACAATCAAGGTTCGCAGTGGTTGTTCTCTCGCCAAATTATGCTTCTTCCAAATGGTGCTTGCTCGAACTCGCAAAGATTGTTCAATGCATGGGTGACAGGGGAACTATTCTGCCAATCTTTTATGAAGTGGATCCCTCTAATGTAAGACATCAGAAACGGAGCTTTGCAGAGGCCTTTGCTGAACACCAAAAAAATTTTGGGGAGGACTCAAACAAGGTGCAAGAGTGGAGAGATGCTTTGACAAAAGTGGCTAATCTCGCTGGCTGGACTTCAAATAACTATAGGTATGAAACTGAGCTTATCAAAGAGATTGTGGAAGCACTGTGGCAGAAAGTTCATCCTACATTTGCATTATCAGAGTCCATAGAGCATTTAGTCGGAATTGATTCCAAATTGATGGAAATAGAGTTTCTTTTAGATACAAGTGCAAATGATGTTTGCTTTATAGGAATATGGGGTATGGGTGGGATCGGGAAGACAACCCTTGCCAGACTTGTTTATCACAGAATTTCCCATCATTTTGAAGTTGGCATATTTCTTACTGATGTCAGACAAGTTTCTGCAAAACATCTACAAGAACAACTTCTTTGCCAGATCTTGAAGGAAATAAATGTTGAAGTACGAGATGTATATGATGGAATGACCAAGATAAAGAGATGTTTATTCAATAAAAAGGTTCTTCTAGTTATTGATGATGTGGATCAGTTAGAACAACTGGAAATGTTGGCTCGAGAAAAAGACTGGTTTGGTTCAGGAAGCATAATTATCGTTACAACAAGAGATCAACGTTTACTAGACTCACGTGATATAGAGAGACAATATAAGAGCAAGGGATTAAATGAGCTTGAAGCTCTTCAGCTCTTTAGTTGGAAAGCATTTAAGAAAGATAAACCTGAAGAAGGTTACAGGGAACTGTCTGAGAGTATCTTGGATTATGCCAAGGGCCTTCCGTTAGCTCTTAAGATTTTGGGATCTAGCTTTTCTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

404

Amino Acids

46.37

Weight (kDa)

6.54

Isoelectric Point (pI)

37.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 22 - 203 2.7e-56 TIR domain
TIR_2 PF13676 25 - 121 4.3e-16 TIR domain
NB-ARC PF00931 218 - 371 1.5e-23 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 103, 145
AclI AACGTT 1 cut(s) 1012
AclWI GGATC 5 cut(s) 350, 363, 701, 933, 1204
AcsI RAATTY 2 cut(s) 418, 732
AcuI CTGAAG 3 cut(s) 164, 1064, 1140
AcvI CACGTG 1 cut(s) 1028
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 190, 846
AfiI CCNNNNNNNGG 1 cut(s) 1124
AflII CTTAAG 1 cut(s) 1184
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 8 cut(s) 224, 308, 498, 749, 826, 842, 892, 1073
AjiI CACGTC 2 cut(s) 33, 73
AjnI CCWGG 1 cut(s) 210
AjuI GAANNNNNNNTTGG 2 cut(s) 250, 282
AluBI AGCT 8 cut(s) 12, 387, 523, 1069, 1076, 1084, 1181, 1203
AluI AGCT 8 cut(s) 12, 387, 523, 1069, 1076, 1084, 1181, 1203
Alw26I GTCTC 2 cut(s) 159, 1033
AlwI GGATC 5 cut(s) 350, 363, 701, 933, 1204
Ama87I CYCGRG 1 cut(s) 954
AoxI GGCC 2 cut(s) 396, 1168
ApeKI GCWGC 1 cut(s) 215
ApoI RAATTY 2 cut(s) 418, 732
Asp700I GAANNNNTTC 3 cut(s) 610, 807, 1077
AspS9I GGNCC 2 cut(s) 118, 1168
AsuHPI GGTGA 2 cut(s) 104, 329
AvaI CYCGRG 1 cut(s) 954
AvaII GGWCC 1 cut(s) 118
BamHI GGATCC 1 cut(s) 355
BbrPI CACGTG 1 cut(s) 1028
BbsI GAAGAC 1 cut(s) 707
BbvI GCAGC 1 cut(s) 202
BccI CCATC 3 cut(s) 616, 747, 854
BcgI CGANNNNNNTGC 2 cut(s) 973, 1007
BciT130I CCWGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 159, 1033
BfaI CTAG 3 cut(s) 908, 1019, 1200
BfmI CTRYAG 2 cut(s) 13, 505
BfrI CTTAAG 1 cut(s) 1184
BglII AGATCT 2 cut(s) 205, 819
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 212
Bme18I GGWCC 1 cut(s) 118
BmeT110I CYCGRG 1 cut(s) 954
BmgBI CACGTC 2 cut(s) 33, 73
BmgT120I GGNCC 2 cut(s) 118, 1168
BmiI GGNNCC 1 cut(s) 357
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 1 cut(s) 448
BpiI GAAGAC 1 cut(s) 707
BpmI CTGGAG 1 cut(s) 186
BsaAI YACGTR 1 cut(s) 1028
BsaBI GATNNNNATC 1 cut(s) 924
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 1164
BsaXI ACNNNNNCTCC 2 cut(s) 184, 214
Bsc4I CCNNNNNNNGG 1 cut(s) 1124
Bse1I ACTGG 2 cut(s) 945, 971
Bse8I GATNNNNATC 1 cut(s) 924
BseBI CCWGG 1 cut(s) 212
BseDI CCNNGG 1 cut(s) 1164
BseGI GGATG 1 cut(s) 562
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 1 cut(s) 1124
BseMII CTCAG 4 cut(s) 11, 71, 510, 1134
BseNI ACTGG 2 cut(s) 945, 971
BseXI GCAGC 1 cut(s) 202
BshFI GGCC 2 cut(s) 398, 1170
BsiHKCI CYCGRG 1 cut(s) 954
BslI CCNNNNNNNGG 1 cut(s) 1124
BsmAI GTCTC 2 cut(s) 159, 1033
BsmBI CGTCTC 1 cut(s) 159
BsnI GGCC 2 cut(s) 398, 1170
BsoBI CYCGRG 1 cut(s) 954
Bsp143I GATC 7 cut(s) 205, 355, 693, 819, 925, 1006, 1196
BspACI CCGC 2 cut(s) 103, 145
BspANI GGCC 2 cut(s) 398, 1170
BspCNI CTCAG 4 cut(s) 12, 72, 511, 1135
BspLI GGNNCC 1 cut(s) 357
BspPI GGATC 5 cut(s) 350, 363, 701, 933, 1204
BspQI GCTCTTC 1 cut(s) 1083
BspTI CTTAAG 1 cut(s) 1184
BsrI ACTGG 2 cut(s) 945, 971
BssECI CCNNGG 1 cut(s) 1164
BssMI GATC 7 cut(s) 205, 355, 693, 819, 925, 1006, 1196
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 1164
Bst2UI CCWGG 1 cut(s) 212
Bst4CI ACNGT 2 cut(s) 549, 1140
Bst6I CTCTTC 1 cut(s) 1083
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 1184
BstBAI YACGTR 1 cut(s) 1028
BstC8I GCNNGC 2 cut(s) 284, 487
BstDEI CTNAG 4 cut(s) 20, 80, 519, 1143
BstENI CCTNNNNNAGG 1 cut(s) 1122
BstF5I GGATG 1 cut(s) 562
BstKTI GATC 7 cut(s) 208, 358, 696, 822, 928, 1009, 1199
BstMAI GTCTC 2 cut(s) 159, 1033
BstMBI GATC 7 cut(s) 205, 355, 693, 819, 925, 1006, 1196
BstMWI GCNNNNNNNGC 1 cut(s) 482
BstNI CCWGG 1 cut(s) 212
BstSCI CCNGG 1 cut(s) 210
BstSFI CTRYAG 2 cut(s) 13, 505
BstV1I GCAGC 1 cut(s) 202
BstV2I GAAGAC 1 cut(s) 707
BstX2I RGATCY 4 cut(s) 205, 355, 819, 1196
BstXI CCANNNNNNTGG 1 cut(s) 622
BstYI RGATCY 4 cut(s) 205, 355, 819, 1196
BsuRI GGCC 2 cut(s) 398, 1170
BtrI CACGTC 2 cut(s) 33, 73
BtsCI GGATG 1 cut(s) 562
BtsI GCAGTG 2 cut(s) 146, 246
BtsIMutI CAGTG 3 cut(s) 146, 246, 545
Cac8I GCNNGC 2 cut(s) 284, 487
Cfr13I GGNCC 2 cut(s) 118, 1168
Csp6I GTAC 2 cut(s) 189, 845
CviAII CATG 1 cut(s) 313
CviQI GTAC 2 cut(s) 189, 845
DdeI CTNAG 4 cut(s) 20, 80, 519, 1143
DpnI GATC 7 cut(s) 207, 357, 695, 821, 927, 1008, 1198
DpnII GATC 7 cut(s) 205, 355, 693, 819, 925, 1006, 1196
Eam1104I CTCTTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 1083
Eco130I CCWWGG 1 cut(s) 1164
Eco147I AGGCCT 1 cut(s) 398
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 3 cut(s) 164, 1064, 1140
Eco72I CACGTG 1 cut(s) 1028
Eco88I CYCGRG 1 cut(s) 954
EcoNI CCTNNNNNAGG 1 cut(s) 1122
EcoO109I RGGNCCY 1 cut(s) 1168
EcoRII CCWGG 1 cut(s) 210
EcoT14I CCWWGG 1 cut(s) 1164
EcoT22I ATGCAT 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 1164
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 1 cut(s) 316
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FatI CATG 1 cut(s) 312
FauI CCCGC 2 cut(s) 110, 138
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 1 cut(s) 549
Fsp4HI GCNGC 1 cut(s) 216
FspBI CTAG 3 cut(s) 908, 1019, 1200
GluI GCNGC 1 cut(s) 216
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 2 cut(s) 398, 1170
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 1 cut(s) 316
HinfI GANTC 5 cut(s) 150, 431, 584, 611, 1022
HphI GGTGA 2 cut(s) 104, 329
Hpy166II GTNNAC 2 cut(s) 114, 1016
Hpy188I TCNGA 7 cut(s) 164, 183, 378, 583, 605, 776, 1144
Hpy188III TCNNGA 6 cut(s) 79, 203, 697, 823, 956, 978
Hpy8I GTNNAC 2 cut(s) 114, 1016
Hpy99I CGWCG 2 cut(s) 10, 74
HpyAV CCTTC 3 cut(s) 820, 1118, 1181
HpyCH4III ACNGT 2 cut(s) 549, 1140
HpyCH4IV ACGT 4 cut(s) 32, 72, 1012, 1027
HpyCH4V TGCA 8 cut(s) 139, 218, 312, 392, 445, 575, 653, 788
HpyF10VI GCNNNNNNNGC 1 cut(s) 482
HpyF3I CTNAG 4 cut(s) 20, 80, 519, 1143
HpySE526I ACGT 4 cut(s) 32, 72, 1012, 1027
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 1 cut(s) 316
Kzo9I GATC 7 cut(s) 205, 355, 693, 819, 925, 1006, 1196
LguI GCTCTTC 1 cut(s) 1083
LmnI GCTCC 1 cut(s) 384
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 1 cut(s) 448
MaeI CTAG 3 cut(s) 908, 1019, 1200
MaeII ACGT 4 cut(s) 32, 72, 1012, 1027
MaeIII GTNAC 3 cut(s) 317, 994, 1127
MalI GATC 7 cut(s) 207, 357, 695, 821, 927, 1008, 1198
MboI GATC 7 cut(s) 205, 355, 693, 819, 925, 1006, 1196
MboII GAAGA 7 cut(s) 107, 170, 261, 712, 896, 1070, 1133
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 4 cut(s) 205, 355, 819, 1196
MluCI AATT 8 cut(s) 134, 219, 259, 418, 606, 617, 732, 987
MlyI GAGTC 3 cut(s) 425, 593, 1016
MmeI TCCRAC 2 cut(s) 583, 1073
MnlI CCTC 5 cut(s) 37, 179, 370, 388, 421
Mph1103I ATGCAT 1 cut(s) 314
MroXI GAANNNNTTC 3 cut(s) 610, 807, 1077
MseI TTAA 3 cut(s) 1061, 1104, 1185
MspCI CTTAAG 1 cut(s) 1184
MspR9I CCNGG 1 cut(s) 212
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 482
NdeII GATC 7 cut(s) 205, 355, 693, 819, 925, 1006, 1196
NlaIII CATG 1 cut(s) 316
NlaIV GGNNCC 1 cut(s) 357
NmuCI GTSAC 1 cut(s) 317
NsiI ATGCAT 1 cut(s) 314
PaeR7I CTCGAG 1 cut(s) 954
PceI AGGCCT 1 cut(s) 398
PciSI GCTCTTC 1 cut(s) 1083
PdmI GAANNNNTTC 3 cut(s) 610, 807, 1077
PfeI GAWTC 2 cut(s) 150, 611
PkrI GCNGC 1 cut(s) 217
PleI GAGTC 3 cut(s) 425, 592, 1016
PmaCI CACGTG 1 cut(s) 1028
PmlI CACGTG 1 cut(s) 1028
PpsI GAGTC 3 cut(s) 425, 592, 1016
Ppu21I YACGTR 1 cut(s) 1028
Psp1406I AACGTT 1 cut(s) 1012
Psp6I CCWGG 1 cut(s) 210
PspCI CACGTG 1 cut(s) 1028
PspGI CCWGG 1 cut(s) 210
PspN4I GGNNCC 1 cut(s) 357
PspPI GGNCC 2 cut(s) 118, 1168
PsuI RGATCY 4 cut(s) 205, 355, 819, 1196
RsaI GTAC 2 cut(s) 190, 846
RsaNI GTAC 2 cut(s) 189, 845
SapI GCTCTTC 1 cut(s) 1083
SaqAI TTAA 3 cut(s) 1061, 1104, 1185
SatI GCNGC 1 cut(s) 216
Sau3AI GATC 7 cut(s) 205, 355, 693, 819, 925, 1006, 1196
Sau96I GGNCC 2 cut(s) 118, 1168
SchI GAGTC 3 cut(s) 425, 593, 1016
ScrFI CCNGG 1 cut(s) 212
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 2 cut(s) 13, 505
Sfr274I CTCGAG 1 cut(s) 954
SinI GGWCC 1 cut(s) 118
SlaI CTCGAG 1 cut(s) 954
SmlI CTYRAG 2 cut(s) 954, 1184
SmoI CTYRAG 2 cut(s) 954, 1184
Sse9I AATT 8 cut(s) 134, 219, 259, 418, 606, 617, 732, 987
SseBI AGGCCT 1 cut(s) 398
SsiI CCGC 2 cut(s) 103, 145
SspMI CTAG 3 cut(s) 908, 1019, 1200
StuI AGGCCT 1 cut(s) 398
StyD4I CCNGG 1 cut(s) 210
StyI CCWWGG 1 cut(s) 1164
TaaI ACNGT 2 cut(s) 549, 1140
TaiI ACGT 4 cut(s) 35, 75, 1015, 1030
TaqI TCGA 3 cut(s) 8, 288, 955
TasI AATT 8 cut(s) 134, 219, 259, 418, 606, 617, 732, 987
TfiI GAWTC 2 cut(s) 150, 611
Tru1I TTAA 3 cut(s) 1061, 1104, 1185
Tru9I TTAA 3 cut(s) 1061, 1104, 1185
TscAI CASTG 3 cut(s) 146, 246, 552
TseFI GTSAC 1 cut(s) 317
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 317
TspDTI ATGAA 4 cut(s) 30, 363, 528, 551
TspGWI ACGGA 3 cut(s) 131, 397, 1164
TspRI CASTG 3 cut(s) 146, 246, 552
Vha464I CTTAAG 1 cut(s) 1184
VpaK11BI GGWCC 1 cut(s) 118
XagI CCTNNNNNAGG 1 cut(s) 1122
XapI RAATTY 2 cut(s) 418, 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 419
XhoI CTCGAG 1 cut(s) 954
XmnI GAANNNNTTC 3 cut(s) 610, 807, 1077
XspI CTAG 3 cut(s) 908, 1019, 1200
Zsp2I ATGCAT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.