MD02G1167500.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0001271
Physical Location & Seq
Forward (+)
14361288 .. 14365816
4529 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1167500.v1.1.491

Sequence Viewer

Length: 1941 bp
ATGGTTTCCACCATGCGTTGGCTGTCTAACATTACCTTCTCTTCTTCTTCCTCCTCCTCGTCGGCGAAAGAGGAGTCGCCGAAAAAGAAGGCCAGCGATGACGGGGGAGGCTGGATGTTTCGGTCGAGGAGGAAGCTCACCCGACAGAGGAAGCAGCTTTTGATCGGTATTGGCGGTGGAAACCATGAAGGTGGAGGAGGTAGAGTCATCAATAATCATAAGCGCTCGGGTTCGAGTTCGTCGTTTCCGGAAACTACGTCTGGGGCAGGGACTCCTCAGCCGCTGCCATTGCCGGAATCGACGGCGGATTTGCGATGCAAAGAGAGGTGCCGGAGAGAGAGGTGCTCCGGATGCAGTTCCGGCGATTGTCGATTGCCGTCTCCCAAGGCCGGTGACGCTACCTCCTCCAACTCTTCTATCCAGAGTGTGTTCGCTTGCCGGGAAAGGCGAAAGAATAACGAGCATTGTTTTGAACCTAAGTCAAGGTCTACGAGGAGGAATCGAGGTAGGAAAGGTTTAGAGAGCTATCCTAGTGACTTCCGGCTTGATGTTCCGAAACCTACCTGGAGTGCTCCGGCTAGTCCTTTCTCAAGTCCTACATTCAGCCCGCACAGACCCAATGCTGGTGATCTGCTCCCACACCTTGTTCCTGTTGGAAATCAAGGCTGGTCTGCCCCCGAGATGCCCACTTGCGATGTGCCTTTTGCGCACTCCCCCCCTTCCTTCTCCGAATACTCCATCCAGAGCAATGATAATTCCCCTCTACAAAGTCCATCCAATAGAAGTCCCTGCCGAAACGTCAGAAGCCCACCAGGTTCAACATCGCCATTGCATCCAACATTATCCTTTGAAGCCCCCACTGTGCGGCGTGAGGTTAATGGTTATGCTGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGTCTTAGCCTACCATCACCACAAGCTATACTCACACCTAAACCAGAGTCCCAGCCAGTCAAAGGTCAATGGCAAAAGGGAAAGCTTATTGGACGTGGCACGTTCGGAAGTGTTTATGTTGCTACCAATAGAGAAACTGGAGCGTTATGTGCAATGAAGGAAGTCGAGTTATTTCCTGAGGACCCAAAATCTGCAGAGTGTATAAAGCAGCTACAGCAGGAAATCAAAGTTCTCAGCCAGCTGAAGCATCCTAATATTGTGCAGTACTATGGAAGTGAAATAGTTGATGATCGATTTTTCATCTATCTGGAGTATGTCCATCCTGGTTCTATTAACAAATATGTCCATGAACATTGTGGAGCCATAACAGAAGCTGTTGTTCGTAATTTTACTCGCCATATTCTCTCTGGGTTGGCTTACTTGCACAGCACAAAGACAATACACAGGGACATCAAAGGGGCTAATTTGCTTGTCGATTCATGTGGAGTTGTCAAGCTAGCTGACTTTGGAATGGCAAAACATCTTTCGGGACACGTAGGTAATCTTTCTCTAAAGGGAAGCCCGTACTGGATGGCTCCAGAGCTGATGCAAGCTGTGATGCATAAAGATAGCAGCTCTGATCTGGCTCTTGCTGTTGATATATGGAGCTTGGGTTCCACTATTATTGAAATGTTCACCGGAAAACCTCCTTGGAGTGAGTACGAAGGGGCTGCAGCCATGTTTAAAGTTATGAAGGATGCGCCACCAATTCCTGAAACATTGTCGCCTGAGGGTAAGGATTTCTTAAGATGCTGCTTCCAAAGAAATCCTGCAGAAAGGCCAACTGCTGCCATTTTATTAGAACATCGATTCTTAAAGAACTCACAGCAGCAAGATGCCCCGCCCACCGCCCAAGCATCTAATGGGGTGAATTACGCGGATAAACCTCTGAGTCCAATGGAACTTGAAAGAAAATTTGATCAGTCGCTGGTGATTCCGGCCATACTTGGATCGCAGAGCAGTGCATTCCCGATTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

647

Amino Acids

70.75

Weight (kDa)

9.11

Isoelectric Point (pI)

67.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 331 - 592 7.5e-66 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 334 - 587 5e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 708
AccB1I GGYRCC 1 cut(s) 327
AccB7I CCANNNNNTGG 1 cut(s) 18
AccI GTMKAC 1 cut(s) 488
AccII CGCG 1 cut(s) 1838
AccIII TCCGGA 2 cut(s) 247, 347
AciI CCGC 8 cut(s) 174, 281, 305, 608, 865, 1802, 1809, 1838
AclWI GGATC 1 cut(s) 1918
AcoI YGGCCR 1 cut(s) 1899
AcsI RAATTY 1 cut(s) 1874
AcuI CTGAAG 2 cut(s) 909, 1184
AfaI GTAC 3 cut(s) 1187, 1487, 1622
AfeI AGCGCT 1 cut(s) 224
AfiI CCNNNNNNNGG 6 cut(s) 18, 147, 389, 623, 864, 983
AflII CTTAAG 1 cut(s) 1705
AflIII ACRYGT 1 cut(s) 1453
AgsI TTSAA 5 cut(s) 473, 819, 851, 1589, 1868
AjiI CACGTC 1 cut(s) 1016
AjnI CCWGG 4 cut(s) 563, 811, 913, 1243
AjuI GAANNNNNNNTTGG 2 cut(s) 1594, 1626
AloI GAACNNNNNNTCC 2 cut(s) 1558, 1590
Alw21I GWGCWC 2 cut(s) 347, 574
Alw26I GTCTC 1 cut(s) 384
AlwI GGATC 1 cut(s) 1918
AlwNI CAGNNNCTG 3 cut(s) 283, 1295, 1888
Ama87I CYCGRG 2 cut(s) 226, 677
Aor13HI TCCGGA 2 cut(s) 247, 347
Aor51HI AGCGCT 1 cut(s) 224
AoxI GGCC 4 cut(s) 90, 387, 1739, 1899
ApeKI GCWGC 9 cut(s) 154, 283, 1129, 1533, 1631, 1634, 1713, 1748, 1789
ApoI RAATTY 1 cut(s) 1874
ArsI GACNNNNNNTTYG 4 cut(s) 59, 91, 292, 324
AspLEI GCGC 3 cut(s) 225, 709, 1663
AspS9I GGNCC 1 cut(s) 1102
AsuC2I CCSGG 1 cut(s) 440
AsuHPI GGTGA 7 cut(s) 130, 404, 638, 930, 1588, 1840, 1903
AsuNHI GCTAGC 1 cut(s) 1417
AvaI CYCGRG 2 cut(s) 226, 677
AvaII GGWCC 1 cut(s) 1102
AxyI CCTNAGG 2 cut(s) 1098, 1689
BanI GGYRCC 1 cut(s) 327
Bbv12I GWGCWC 2 cut(s) 347, 574
BbvCI CCTCAGC 1 cut(s) 276
BbvI GCAGC 9 cut(s) 166, 270, 1141, 1545, 1618, 1646, 1700, 1735, 1801
BccI CCATC 6 cut(s) 746, 781, 903, 943, 1248, 1486
BceAI ACGGC 2 cut(s) 318, 361
BcgI CGANNNNNNTGC 2 cut(s) 1613, 1647
BciT130I CCWGG 4 cut(s) 565, 813, 915, 1245
BclI TGATCA 1 cut(s) 1879
BcnI CCSGG 1 cut(s) 440
BcoDI GTCTC 1 cut(s) 384
BfaI CTAG 3 cut(s) 531, 579, 1418
BfmI CTRYAG 4 cut(s) 1113, 1133, 1632, 1731
BfoI RGCGCY 1 cut(s) 226
BfrI CTTAAG 1 cut(s) 1705
BmcAI AGTACT 1 cut(s) 1187
Bme1390I CCNGG 5 cut(s) 440, 565, 813, 915, 1245
Bme18I GGWCC 1 cut(s) 1102
BmeT110I CYCGRG 2 cut(s) 226, 677
BmgBI CACGTC 1 cut(s) 1016
BmgT120I GGNCC 1 cut(s) 1102
BmiI GGNNCC 5 cut(s) 329, 1104, 1282, 1497, 1576
BmrFI CCNGG 5 cut(s) 440, 565, 813, 915, 1245
BmtI GCTAGC 1 cut(s) 1421
BplI GAGNNNNNCTC 2 cut(s) 329, 361
BpmI CTGGAG 5 cut(s) 586, 936, 1080, 1250, 1482
Bpu10I CCTNAGC 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 574
BpuMI CCSGG 1 cut(s) 440
Bsa29I ATCGAT 2 cut(s) 1213, 1768
BsaAI YACGTR 1 cut(s) 1456
BsaJI CCNNGG 2 cut(s) 384, 1610
BsaWI WCCGGW 3 cut(s) 247, 347, 1598
BsaXI ACNNNNNCTCC 6 cut(s) 189, 219, 386, 416, 1558, 1588
Bsc4I CCNNNNNNNGG 6 cut(s) 18, 147, 389, 623, 864, 983
Bse118I RCCGGY 1 cut(s) 389
Bse1I ACTGG 3 cut(s) 977, 1063, 1493
Bse21I CCTNAGG 2 cut(s) 1098, 1689
Bse3DI GCAATG 5 cut(s) 287, 754, 827, 899, 1080
BseAI TCCGGA 2 cut(s) 247, 347
BseBI CCWGG 4 cut(s) 565, 813, 915, 1245
BseCI ATCGAT 2 cut(s) 1213, 1768
BseDI CCNNGG 2 cut(s) 384, 1610
BseLI CCNNNNNNNGG 6 cut(s) 18, 147, 389, 623, 864, 983
BseMI GCAATG 5 cut(s) 287, 754, 827, 899, 1080
BseMII CTCAG 5 cut(s) 290, 1089, 1168, 1680, 1841
BseNI ACTGG 3 cut(s) 977, 1063, 1493
BseRI GAGGAG 8 cut(s) 43, 46, 86, 142, 210, 264, 394, 508
BseXI GCAGC 9 cut(s) 166, 270, 1141, 1545, 1618, 1646, 1700, 1735, 1801
BseYI CCCAGC 1 cut(s) 972
BsgI GTGCAG 1 cut(s) 1202
Bsh1236I CGCG 1 cut(s) 1838
Bsh1285I CGRYCG 1 cut(s) 125
BshFI GGCC 4 cut(s) 92, 389, 1741, 1901
BshNI GGYRCC 1 cut(s) 327
BshVI ATCGAT 2 cut(s) 1213, 1768
BsiEI CGRYCG 1 cut(s) 125
BsiHKAI GWGCWC 2 cut(s) 347, 574
BsiHKCI CYCGRG 2 cut(s) 226, 677
BslFI GGGAC 5 cut(s) 283, 771, 955, 1382, 1464
BslI CCNNNNNNNGG 6 cut(s) 18, 147, 389, 623, 864, 983
BsmAI GTCTC 1 cut(s) 384
BsmBI CGTCTC 1 cut(s) 384
BsmFI GGGAC 5 cut(s) 283, 771, 955, 1382, 1464
BsmI GAATGC 1 cut(s) 1925
BsnI GGCC 4 cut(s) 92, 389, 1741, 1901
BsoBI CYCGRG 2 cut(s) 226, 677
Bsp1286I GDGCHC 2 cut(s) 347, 574
Bsp13I TCCGGA 2 cut(s) 247, 347
Bsp143I GATC 6 cut(s) 162, 628, 1210, 1540, 1879, 1910
BspACI CCGC 8 cut(s) 174, 281, 305, 608, 865, 1802, 1809, 1838
BspANI GGCC 4 cut(s) 92, 389, 1741, 1901
BspCNI CTCAG 5 cut(s) 289, 1090, 1167, 1681, 1842
BspDI ATCGAT 2 cut(s) 1213, 1768
BspEI TCCGGA 2 cut(s) 247, 347
BspFNI CGCG 1 cut(s) 1838
BspLI GGNNCC 5 cut(s) 329, 1104, 1282, 1497, 1576
BspMAI CTGCAG 3 cut(s) 1117, 1636, 1735
BspOI GCTAGC 1 cut(s) 1421
BspPI GGATC 1 cut(s) 1918
BspT107I GGYRCC 1 cut(s) 327
BspTI CTTAAG 1 cut(s) 1705
BsrDI GCAATG 5 cut(s) 287, 754, 827, 899, 1080
BsrFI RCCGGY 1 cut(s) 389
BsrI ACTGG 3 cut(s) 977, 1063, 1493
BssAI RCCGGY 1 cut(s) 389
BssECI CCNNGG 2 cut(s) 384, 1610
BssMI GATC 6 cut(s) 162, 628, 1210, 1540, 1879, 1910
BssT1I CCWWGG 2 cut(s) 384, 1610
Bst2UI CCWGG 4 cut(s) 565, 813, 915, 1245
Bst4CI ACNGT 1 cut(s) 862
Bst6I CTCTTC 2 cut(s) 46, 418
BstAFI CTTAAG 1 cut(s) 1705
BstBAI YACGTR 1 cut(s) 1456
BstC8I GCNNGC 6 cut(s) 94, 436, 608, 1160, 1419, 1512
BstDEI CTNAG 7 cut(s) 276, 477, 926, 1098, 1154, 1689, 1850
BstFNI CGCG 1 cut(s) 1838
BstH2I RGCGCY 1 cut(s) 226
BstHHI GCGC 3 cut(s) 225, 709, 1663
BstKTI GATC 6 cut(s) 165, 631, 1213, 1543, 1882, 1913
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 6 cut(s) 162, 628, 1210, 1540, 1879, 1910
BstMCI CGRYCG 1 cut(s) 125
BstMWI GCNNNNNNNGC 7 cut(s) 289, 351, 360, 395, 706, 1070, 1135
BstNI CCWGG 4 cut(s) 565, 813, 915, 1245
BstSCI CCNGG 5 cut(s) 438, 563, 811, 913, 1243
BstSFI CTRYAG 4 cut(s) 1113, 1133, 1632, 1731
BstUI CGCG 1 cut(s) 1838
BstV1I GCAGC 9 cut(s) 166, 270, 1141, 1545, 1618, 1646, 1700, 1735, 1801
BstXI CCANNNNNNTGG 1 cut(s) 191
Bsu15I ATCGAT 2 cut(s) 1213, 1768
Bsu36I CCTNAGG 2 cut(s) 1098, 1689
BsuRI GGCC 4 cut(s) 92, 389, 1741, 1901
BsuTUI ATCGAT 2 cut(s) 1213, 1768
BtgZI GCGATG 4 cut(s) 111, 328, 708, 807
BtrI CACGTC 1 cut(s) 1016
BtsI GCAGTG 1 cut(s) 1927
BtsIMutI CAGTG 2 cut(s) 858, 1927
Cac8I GCNNGC 6 cut(s) 94, 436, 608, 1160, 1419, 1512
CaiI CAGNNNCTG 3 cut(s) 283, 1295, 1888
CfoI GCGC 3 cut(s) 225, 709, 1663
Cfr10I RCCGGY 1 cut(s) 389
Cfr13I GGNCC 1 cut(s) 1102
ClaI ATCGAT 2 cut(s) 1213, 1768
CseI GACGC 1 cut(s) 404
CsiI ACCWGGT 1 cut(s) 811
Csp6I GTAC 3 cut(s) 1186, 1486, 1621
CspCI CAANNNNNGTGG 2 cut(s) 1567, 1602
CviAII CATG 5 cut(s) 13, 185, 1268, 1401, 1639
CviQI GTAC 3 cut(s) 1186, 1486, 1621
DdeI CTNAG 7 cut(s) 276, 477, 926, 1098, 1154, 1689, 1850
DpnI GATC 6 cut(s) 164, 630, 1212, 1542, 1881, 1912
DpnII GATC 6 cut(s) 162, 628, 1210, 1540, 1879, 1910
DraI TTTAAA 1 cut(s) 1645
EaeI YGGCCR 1 cut(s) 1899
Eam1104I CTCTTC 2 cut(s) 46, 418
EarI CTCTTC 2 cut(s) 46, 418
EciI GGCGGA 1 cut(s) 320
Eco130I CCWWGG 2 cut(s) 384, 1610
Eco47I GGWCC 1 cut(s) 1102
Eco47III AGCGCT 1 cut(s) 224
Eco57I CTGAAG 2 cut(s) 909, 1184
Eco81I CCTNAGG 2 cut(s) 1098, 1689
Eco88I CYCGRG 2 cut(s) 226, 677
EcoO109I RGGNCCY 1 cut(s) 1102
EcoRII CCWGG 4 cut(s) 563, 811, 913, 1243
EcoT14I CCWWGG 2 cut(s) 384, 1610
EcoT22I ATGCAT 1 cut(s) 1524
ErhI CCWWGG 2 cut(s) 384, 1610
Esp3I CGTCTC 1 cut(s) 384
FaeI CATG 5 cut(s) 16, 188, 1271, 1404, 1642
FalI AAGNNNNNCTT 2 cut(s) 1688, 1720
FaqI GGGAC 5 cut(s) 283, 771, 955, 1382, 1464
FatI CATG 5 cut(s) 12, 184, 1267, 1400, 1638
FauI CCCGC 2 cut(s) 615, 1809
FbaI TGATCA 1 cut(s) 1879
FblI GTMKAC 1 cut(s) 488
FspBI CTAG 3 cut(s) 531, 579, 1418
FspI TGCGCA 1 cut(s) 708
GlaI GCGC 3 cut(s) 224, 708, 1662
GsaI CCCAGC 1 cut(s) 976
GsuI CTGGAG 5 cut(s) 586, 936, 1080, 1250, 1482
HaeII RGCGCY 1 cut(s) 226
HaeIII GGCC 4 cut(s) 92, 389, 1741, 1901
HgaI GACGC 1 cut(s) 404
HhaI GCGC 3 cut(s) 225, 709, 1663
Hin1II CATG 5 cut(s) 16, 188, 1271, 1404, 1642
Hin6I GCGC 3 cut(s) 223, 707, 1661
HinP1I GCGC 3 cut(s) 223, 707, 1661
HindIII AAGCTT 1 cut(s) 1004
HphI GGTGA 7 cut(s) 130, 404, 638, 930, 1588, 1840, 1903
Hpy166II GTNNAC 2 cut(s) 489, 1596
Hpy188I TCNGA 6 cut(s) 555, 730, 803, 1028, 1540, 1851
Hpy8I GTNNAC 2 cut(s) 489, 1596
Hpy99I CGWCG 3 cut(s) 64, 244, 304
HpyAV CCTTC 8 cut(s) 46, 82, 182, 729, 733, 1072, 1619, 1648
HpyCH4III ACNGT 1 cut(s) 862
HpyCH4IV ACGT 5 cut(s) 257, 798, 1015, 1022, 1455
HpyF10VI GCNNNNNNNGC 7 cut(s) 289, 351, 360, 395, 706, 1070, 1135
HpyF3I CTNAG 7 cut(s) 276, 477, 926, 1098, 1154, 1689, 1850
HpySE526I ACGT 5 cut(s) 257, 798, 1015, 1022, 1455
Hsp92II CATG 5 cut(s) 16, 188, 1271, 1404, 1642
HspAI GCGC 3 cut(s) 223, 707, 1661
Kpn2I TCCGGA 2 cut(s) 247, 347
Ksp22I TGATCA 1 cut(s) 1879
Kzo9I GATC 6 cut(s) 162, 628, 1210, 1540, 1879, 1910
LmnI GCTCC 8 cut(s) 350, 577, 639, 917, 1061, 1280, 1501, 1566
Lsp1109I GCAGC 9 cut(s) 166, 270, 1141, 1545, 1618, 1646, 1700, 1735, 1801
MabI ACCWGGT 1 cut(s) 811
MaeI CTAG 3 cut(s) 531, 579, 1418
MaeII ACGT 5 cut(s) 257, 798, 1015, 1022, 1455
MaeIII GTNAC 2 cut(s) 392, 533
MalI GATC 6 cut(s) 164, 630, 1212, 1542, 1881, 1912
MboI GATC 6 cut(s) 162, 628, 1210, 1540, 1879, 1910
MboII GAAGA 4 cut(s) 33, 36, 39, 405
MhlI GDGCHC 2 cut(s) 347, 574
MluCI AATT 6 cut(s) 754, 1306, 1384, 1668, 1831, 1874
MlyI GAGTC 5 cut(s) 83, 213, 265, 977, 1861
MmeI TCCRAC 3 cut(s) 432, 634, 860
Mph1103I ATGCAT 1 cut(s) 1524
MroI TCCGGA 2 cut(s) 247, 347
MseI TTAA 5 cut(s) 876, 1254, 1644, 1706, 1775
MslI CAYNNNNRTG 1 cut(s) 189
MspA1I CMGCKG 2 cut(s) 283, 1162
MspCI CTTAAG 1 cut(s) 1705
MspR9I CCNGG 5 cut(s) 440, 565, 813, 915, 1245
Mva1269I GAATGC 1 cut(s) 1925
MvaI CCWGG 4 cut(s) 565, 813, 915, 1245
MvnI CGCG 1 cut(s) 1838
MwoI GCNNNNNNNGC 7 cut(s) 289, 351, 360, 395, 706, 1070, 1135
NciI CCSGG 1 cut(s) 440
NdeII GATC 6 cut(s) 162, 628, 1210, 1540, 1879, 1910
NheI GCTAGC 1 cut(s) 1417
NlaIII CATG 5 cut(s) 16, 188, 1271, 1404, 1642
NlaIV GGNNCC 5 cut(s) 329, 1104, 1282, 1497, 1576
NmuCI GTSAC 2 cut(s) 392, 533
NsbI TGCGCA 1 cut(s) 708
NsiI ATGCAT 1 cut(s) 1524
PcsI WCGNNNNNNNCGW 1 cut(s) 239
PctI GAATGC 1 cut(s) 1925
PfeI GAWTC 5 cut(s) 296, 499, 1397, 1770, 1894
PflMI CCANNNNNTGG 1 cut(s) 18
PfoI TCCNGGA 1 cut(s) 913
PleI GAGTC 5 cut(s) 82, 212, 265, 976, 1860
PpsI GAGTC 5 cut(s) 82, 212, 265, 976, 1860
Ppu21I YACGTR 1 cut(s) 1456
PpuMI RGGWCCY 1 cut(s) 1102
Psp5II RGGWCCY 1 cut(s) 1102
Psp6I CCWGG 4 cut(s) 563, 811, 913, 1243
PspFI CCCAGC 1 cut(s) 972
PspGI CCWGG 4 cut(s) 563, 811, 913, 1243
PspN4I GGNNCC 5 cut(s) 329, 1104, 1282, 1497, 1576
PspPI GGNCC 1 cut(s) 1102
PspPPI RGGWCCY 1 cut(s) 1102
PstI CTGCAG 3 cut(s) 1117, 1636, 1735
PstNI CAGNNNCTG 3 cut(s) 283, 1295, 1888
PvuII CAGCTG 1 cut(s) 1162
RsaI GTAC 3 cut(s) 1187, 1487, 1622
RsaNI GTAC 3 cut(s) 1186, 1486, 1621
RseI CAYNNNNRTG 1 cut(s) 189
SaqAI TTAA 5 cut(s) 876, 1254, 1644, 1706, 1775
Sau3AI GATC 6 cut(s) 162, 628, 1210, 1540, 1879, 1910
Sau96I GGNCC 1 cut(s) 1102
ScaI AGTACT 1 cut(s) 1187
SchI GAGTC 5 cut(s) 83, 213, 265, 977, 1861
ScrFI CCNGG 5 cut(s) 440, 565, 813, 915, 1245
SduI GDGCHC 2 cut(s) 347, 574
SexAI ACCWGGT 1 cut(s) 811
SfcI CTRYAG 4 cut(s) 1113, 1133, 1632, 1731
SinI GGWCC 1 cut(s) 1102
SmiMI CAYNNNNRTG 1 cut(s) 189
SmlI CTYRAG 2 cut(s) 589, 1705
SmoI CTYRAG 2 cut(s) 589, 1705
Sse9I AATT 6 cut(s) 754, 1306, 1384, 1668, 1831, 1874
SsiI CCGC 8 cut(s) 174, 281, 305, 608, 865, 1802, 1809, 1838
SspI AATATT 1 cut(s) 1177
SspMI CTAG 3 cut(s) 531, 579, 1418
StyD4I CCNGG 5 cut(s) 438, 563, 811, 913, 1243
StyI CCWWGG 2 cut(s) 384, 1610
TaaI ACNGT 1 cut(s) 862
TaiI ACGT 5 cut(s) 260, 801, 1018, 1025, 1458
TaqI TCGA 9 cut(s) 125, 233, 299, 370, 502, 1086, 1213, 1395, 1768
TaqII GACCGA 1 cut(s) 111
TasI AATT 6 cut(s) 754, 1306, 1384, 1668, 1831, 1874
TatI WGTACW 1 cut(s) 1185
TauI GCSGC 2 cut(s) 283, 868
TfiI GAWTC 5 cut(s) 296, 499, 1397, 1770, 1894
Tru1I TTAA 5 cut(s) 876, 1254, 1644, 1706, 1775
Tru9I TTAA 5 cut(s) 876, 1254, 1644, 1706, 1775
TscAI CASTG 2 cut(s) 865, 1927
TseFI GTSAC 2 cut(s) 392, 533
TseI GCWGC 9 cut(s) 154, 283, 1129, 1533, 1631, 1634, 1713, 1748, 1789
Tsp45I GTSAC 2 cut(s) 392, 533
TspDTI ATGAA 6 cut(s) 201, 1091, 1210, 1284, 1389, 1667
TspRI CASTG 2 cut(s) 865, 1927
Van91I CCANNNNNTGG 1 cut(s) 18
Vha464I CTTAAG 1 cut(s) 1705
VpaK11BI GGWCC 1 cut(s) 1102
XapI RAATTY 1 cut(s) 1874
XcmI CCANNNNNNNNNTGG 3 cut(s) 1274, 1325, 1820
XmiI GTMKAC 1 cut(s) 488
XspI CTAG 3 cut(s) 531, 579, 1418
ZrmI AGTACT 1 cut(s) 1187
Zsp2I ATGCAT 1 cut(s) 1524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.