Rh2BG180900

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001271
Physical Location & Seq
Forward (+)
16111745 .. 16112996
1252 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG180900.1

Sequence Viewer

Length: 759 bp
ATGTGTTTCAGTGTGTTTTCTTGGCGAGACACTCCAAAGAATAACATTCAAGTACGTGAGACAGTGTCGTCTAGGAGGAAGAATCGAGATCGAAATCATTTACAGATCCCTGAACATGACTTCAGGCTCGATGTTCCTGTTAGAAGTGCACCCACTAGTCCATGCGCAAGTCCTAGATTCAGCCCACGCAGACCAAGTGCTGCTGATTTGTGGGCTCCGTCCAGAAATCAAGTCTGGTCTGCACCGGAGATGACTACTAATGATATGACTCAACCTCCCCCTGCCTTATCAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTTCCACAGGTAGAACTCCCCTTCGACCCCCACAAGGATCATCCCCATTGCATCCCCGGTTATCTGTTACCTCTGAAGTCCATCCATTGCCTCTGCCCCCTGGAGCAAGCCTCCCTTCACCTAGTGTACCATCACCACAACCTGCACTCACACCTAAGCCAGAGTCCCAGCCGATTAGAGGGCAATGGCAAAAGGGAAAGCTTATTGGTCGTGGCACGTTTGGCAGTGTTTATGTTGCTACAAATAGGTACATGCAGTTCCTTGTACAGGAAATCAAAGTCCTCAGCCAGCTCAAGCATCCTAATATTGTGCTGTACTATGGAAGTGAAATTGTGAGTAACGTCTGCTTTTATGTTAAGCATCCAGGCTCATGTTCTTCCATTGGACTACGCCTGAAACAAAACACAAAATACTTTCATGTATATGCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

252

Amino Acids

28.05

Weight (kDa)

10.06

Isoelectric Point (pI)

64.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 67
Acc16I TGCGCA 1 cut(s) 166
Acc36I ACCTGC 2 cut(s) 475, 743
AclWI GGATC 2 cut(s) 100, 370
AcuI CTGAAG 2 cut(s) 106, 420
AdeI CACNNNGTG 1 cut(s) 449
AfaI GTAC 5 cut(s) 54, 453, 575, 591, 641
AfiI CCNNNNNNNGG 3 cut(s) 359, 503, 592
AgsI TTSAA 1 cut(s) 50
AhlI ACTAGT 1 cut(s) 155
AjnI CCWGG 2 cut(s) 424, 688
AluBI AGCT 2 cut(s) 526, 616
AluI AGCT 2 cut(s) 526, 616
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 2 cut(s) 21, 53
Alw44I GTGCAC 1 cut(s) 147
AlwI GGATC 2 cut(s) 100, 370
ApaLI GTGCAC 1 cut(s) 147
ApeKI GCWGC 1 cut(s) 200
AspLEI GCGC 1 cut(s) 167
AsuC2I CCSGG 1 cut(s) 382
AsuHPI GGTGA 2 cut(s) 435, 450
BaeGI GKGCMC 1 cut(s) 151
BanII GRGCYC 1 cut(s) 217
Bbv12I GWGCWC 1 cut(s) 151
BbvCI CCTCAGC 1 cut(s) 608
BbvI GCAGC 1 cut(s) 187
BccI CCATC 2 cut(s) 414, 463
BciT130I CCWGG 2 cut(s) 426, 690
BcnI CCSGG 1 cut(s) 382
BcoDI GTCTC 2 cut(s) 21, 53
BcuI ACTAGT 1 cut(s) 155
BfaI CTAG 4 cut(s) 72, 156, 174, 447
BfuAI ACCTGC 2 cut(s) 475, 743
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme1390I CCNGG 3 cut(s) 382, 426, 690
BmiI GGNNCC 1 cut(s) 216
BmrFI CCNGG 3 cut(s) 382, 426, 690
BmsI GCATC 3 cut(s) 385, 631, 694
BplI GAGNNNNNCTC 2 cut(s) 420, 452
BpmI CTGGAG 1 cut(s) 447
Bpu10I CCTNAGC 2 cut(s) 480, 608
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 382
BsaAI YACGTR 1 cut(s) 56
BsaBI GATNNNNATC 1 cut(s) 93
BsaJI CCNNGG 2 cut(s) 380, 424
BsaWI WCCGGW 1 cut(s) 244
BsaXI ACNNNNNCTCC 2 cut(s) 259, 289
Bsc4I CCNNNNNNNGG 3 cut(s) 359, 503, 592
Bse3DI GCAATG 3 cut(s) 371, 410, 515
Bse8I GATNNNNATC 1 cut(s) 93
BseBI CCWGG 2 cut(s) 426, 690
BseDI CCNNGG 2 cut(s) 380, 424
BseGI GGATG 5 cut(s) 365, 376, 406, 622, 685
BseJI GATNNNNATC 1 cut(s) 93
BseLI CCNNNNNNNGG 3 cut(s) 359, 503, 592
BseMI GCAATG 3 cut(s) 371, 410, 515
BseMII CTCAG 1 cut(s) 622
BseSI GKGCMC 1 cut(s) 151
BseXI GCAGC 1 cut(s) 187
BseYI CCCAGC 1 cut(s) 492
BsgI GTGCAG 2 cut(s) 225, 453
BsiHKAI GWGCWC 1 cut(s) 151
BsiSI CCGG 2 cut(s) 245, 382
BslFI GGGAC 1 cut(s) 475
BslI CCNNNNNNNGG 3 cut(s) 359, 503, 592
BsmAI GTCTC 2 cut(s) 21, 53
BsmFI GGGAC 1 cut(s) 475
Bsp1286I GDGCHC 2 cut(s) 151, 217
Bsp1407I TGTACA 1 cut(s) 589
Bsp143I GATC 3 cut(s) 88, 105, 362
BspCNI CTCAG 1 cut(s) 621
BspLI GGNNCC 1 cut(s) 216
BspMI ACCTGC 2 cut(s) 475, 743
BspPI GGATC 2 cut(s) 100, 370
BsrDI GCAATG 3 cut(s) 371, 410, 515
BsrGI TGTACA 1 cut(s) 589
BssECI CCNNGG 2 cut(s) 380, 424
BssMI GATC 3 cut(s) 88, 105, 362
Bst2UI CCWGG 2 cut(s) 426, 690
Bst4CI ACNGT 2 cut(s) 64, 326
BstAUI TGTACA 1 cut(s) 589
BstBAI YACGTR 1 cut(s) 56
BstC8I GCNNGC 2 cut(s) 433, 614
BstDEI CTNAG 2 cut(s) 480, 608
BstENI CCTNNNNNAGG 1 cut(s) 590
BstF5I GGATG 5 cut(s) 365, 376, 406, 622, 685
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 3 cut(s) 91, 108, 365
BstMAI GTCTC 2 cut(s) 21, 53
BstMBI GATC 3 cut(s) 88, 105, 362
BstMWI GCNNNNNNNGC 1 cut(s) 546
BstNI CCWGG 2 cut(s) 426, 690
BstNSI RCATGY 1 cut(s) 580
BstSCI CCNGG 3 cut(s) 380, 424, 688
BstSLI GKGCMC 1 cut(s) 151
BstV1I GCAGC 1 cut(s) 187
BstX2I RGATCY 1 cut(s) 105
BstYI RGATCY 1 cut(s) 105
BtsCI GGATG 5 cut(s) 365, 376, 406, 622, 685
BtsI GCAGTG 1 cut(s) 556
BtsIMutI CAGTG 3 cut(s) 16, 69, 556
BveI ACCTGC 2 cut(s) 475, 743
Cac8I GCNNGC 2 cut(s) 433, 614
CfoI GCGC 1 cut(s) 167
Csp6I GTAC 5 cut(s) 53, 452, 574, 590, 640
CviAII CATG 5 cut(s) 116, 162, 577, 696, 743
CviQI GTAC 5 cut(s) 53, 452, 574, 590, 640
DdeI CTNAG 2 cut(s) 480, 608
DpnI GATC 3 cut(s) 90, 107, 364
DpnII GATC 3 cut(s) 88, 105, 362
DraIII CACNNNGTG 1 cut(s) 449
DrdI GACNNNNNNGTC 1 cut(s) 67
DseDI GACNNNNNNGTC 1 cut(s) 67
Eco24I GRGCYC 1 cut(s) 217
Eco57I CTGAAG 2 cut(s) 106, 420
EcoNI CCTNNNNNAGG 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 424, 688
EcoT38I GRGCYC 1 cut(s) 217
FaeI CATG 5 cut(s) 119, 165, 580, 699, 746
FalI AAGNNNNNCTT 2 cut(s) 424, 456
FaqI GGGAC 1 cut(s) 475
FatI CATG 5 cut(s) 115, 161, 576, 695, 742
Fnu4HI GCNGC 1 cut(s) 201
FokI GGATG 5 cut(s) 352, 363, 393, 609, 672
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 1 cut(s) 201
FspBI CTAG 4 cut(s) 72, 156, 174, 447
FspI TGCGCA 1 cut(s) 166
GlaI GCGC 1 cut(s) 166
GluI GCNGC 1 cut(s) 201
GsaI CCCAGC 1 cut(s) 496
GsuI CTGGAG 1 cut(s) 447
HapII CCGG 2 cut(s) 245, 382
HhaI GCGC 1 cut(s) 167
Hin1II CATG 5 cut(s) 119, 165, 580, 699, 746
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HindIII AAGCTT 1 cut(s) 524
HinfI GANTC 4 cut(s) 82, 177, 268, 488
HpaII CCGG 2 cut(s) 245, 382
HphI GGTGA 2 cut(s) 435, 450
Hpy166II GTNNAC 2 cut(s) 149, 452
Hpy188I TCNGA 2 cut(s) 292, 400
Hpy188III TCNNGA 2 cut(s) 86, 222
Hpy8I GTNNAC 2 cut(s) 149, 452
HpyAV CCTTC 2 cut(s) 356, 450
HpyCH4III ACNGT 2 cut(s) 64, 326
HpyCH4IV ACGT 3 cut(s) 55, 542, 666
HpyCH4V TGCA 6 cut(s) 149, 242, 376, 470, 580, 752
HpyF10VI GCNNNNNNNGC 1 cut(s) 546
HpyF3I CTNAG 2 cut(s) 480, 608
HpySE526I ACGT 3 cut(s) 55, 542, 666
Hsp92II CATG 5 cut(s) 119, 165, 580, 699, 746
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 3 cut(s) 88, 105, 362
LmnI GCTCC 2 cut(s) 220, 428
Lsp1109I GCAGC 1 cut(s) 187
LweI GCATC 3 cut(s) 385, 631, 694
MaeI CTAG 4 cut(s) 72, 156, 174, 447
MaeII ACGT 3 cut(s) 55, 542, 666
MaeIII GTNAC 2 cut(s) 391, 662
MalI GATC 3 cut(s) 90, 107, 364
MboI GATC 3 cut(s) 88, 105, 362
MboII GAAGA 2 cut(s) 91, 693
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 2 cut(s) 151, 217
MluCI AATT 1 cut(s) 654
MlyI GAGTC 2 cut(s) 262, 497
MnlI CCTC 7 cut(s) 69, 285, 406, 426, 446, 497, 617
MseI TTAA 1 cut(s) 681
MslI CAYNNNNRTG 1 cut(s) 747
MspI CCGG 2 cut(s) 245, 382
MspR9I CCNGG 3 cut(s) 382, 426, 690
MvaI CCWGG 2 cut(s) 426, 690
MwoI GCNNNNNNNGC 1 cut(s) 546
NciI CCSGG 1 cut(s) 382
NdeII GATC 3 cut(s) 88, 105, 362
NlaIII CATG 5 cut(s) 119, 165, 580, 699, 746
NlaIV GGNNCC 1 cut(s) 216
NsbI TGCGCA 1 cut(s) 166
NspI RCATGY 1 cut(s) 580
PfeI GAWTC 2 cut(s) 82, 177
PflFI GACNNNGTC 1 cut(s) 64
PkrI GCNGC 1 cut(s) 202
PleI GAGTC 2 cut(s) 262, 496
PpsI GAGTC 2 cut(s) 262, 496
Ppu21I YACGTR 1 cut(s) 56
Psp6I CCWGG 2 cut(s) 424, 688
PspFI CCCAGC 1 cut(s) 492
PspGI CCWGG 2 cut(s) 424, 688
PspN4I GGNNCC 1 cut(s) 216
PsrI GAACNNNNNNTAC 4 cut(s) 295, 327, 566, 598
PsuI RGATCY 1 cut(s) 105
PsyI GACNNNGTC 1 cut(s) 64
RsaI GTAC 5 cut(s) 54, 453, 575, 591, 641
RsaNI GTAC 5 cut(s) 53, 452, 574, 590, 640
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 1 cut(s) 681
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 3 cut(s) 88, 105, 362
SchI GAGTC 2 cut(s) 262, 497
ScrFI CCNGG 3 cut(s) 382, 426, 690
SduI GDGCHC 2 cut(s) 151, 217
SfaNI GCATC 3 cut(s) 385, 631, 694
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SpeI ACTAGT 1 cut(s) 155
Sse9I AATT 1 cut(s) 654
SspI AATATT 1 cut(s) 631
SspMI CTAG 4 cut(s) 72, 156, 174, 447
StyD4I CCNGG 3 cut(s) 380, 424, 688
TaaI ACNGT 2 cut(s) 64, 326
TaiI ACGT 3 cut(s) 58, 545, 669
TaqI TCGA 4 cut(s) 85, 91, 129, 349
TasI AATT 1 cut(s) 654
TatI WGTACW 2 cut(s) 589, 639
TfiI GAWTC 2 cut(s) 82, 177
Tru1I TTAA 1 cut(s) 681
Tru9I TTAA 1 cut(s) 681
TscAI CASTG 3 cut(s) 16, 69, 556
TseI GCWGC 1 cut(s) 200
TspDTI ATGAA 2 cut(s) 321, 731
TspGWI ACGGA 1 cut(s) 207
TspRI CASTG 3 cut(s) 16, 69, 556
Tth111I GACNNNGTC 1 cut(s) 64
VneI GTGCAC 1 cut(s) 147
XagI CCTNNNNNAGG 1 cut(s) 590
XceI RCATGY 1 cut(s) 580
XspI CTAG 4 cut(s) 72, 156, 174, 447
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.