MD15G1279700.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001271
Physical Location & Seq
Forward (+)
24878464 .. 24882717
4254 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1279700.v1.1.491

Sequence Viewer

Length: 1863 bp
ATGGTTTCCACCATGGGTTGGCTGTCTAACATTACCTTCTCTTCTTCTTCCTCCTCGTCGGCATCGTCGTCTTCGCCGTTCAGATCGGCAAACGGGGAGTCGCCTAAAAAGAAGACCAGCGATGACGAGAGAGGGTGGATTTTCCGGTCGAGGAGGAAGCTCACCCGACAGAGGAAGCAGCTTTTGATCTGTACTGGCGGCGGAGACCAGGACGGGGGAGGTGGAGCCGTGAATAGTCATAAATGCTCGGGTTCGAGTTCGTCGCTTCCGGAAACCACGTCCGCGGCCGGGACTCCTCAGCCGCTGCCTTTGCCGGAATTGGCAGCGGATTCACGATGGAAAGAGAGTGTGTTTGGTGGCCGGGAAGCGCGAAAAAATAACGAGAATTGTTTTGAATCAAGGTCAAGGTCTACGAGGAGGAATCAAGGTAGGAATGGTTTAGAGAGCTATCATACTGACTTCGGGCTTGAAGTTCCAAATCGTATTTGGAGCGCACCTGCTAGCCCTTTTTCGAGTCCTACATTCAGCCCACACAGTCCCAGTGCTGGTGATCTTCTCCCACACCTTGTTCCTGTGGGAAATCAAGGCTGGTCTGTCCCCGAGATGCCCACTTGCGATATGACTTCTGTGCTCCCTCCCCCTTCCTTGTCGGAATACTCCACCCAGAGTAATGATAATTCCCCTATGCAAAGTCCATCAAATAGAAGTCCCTGCCGAAATGTCAAAATCCCACCAGGCTCAACATCGCCATTGCCTCCCAGATTATCAATTGAAGTCTCCACTGCGCGGCGTGAGGTCAATGGTTATGCTGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGTCTTAGCCTACCTTCACCACACGCTATACCCACACCTAAACCGGAGTCCCAGCCAGTGAAAGGACAATGGCAAAAGGGAAAGCTTATTGGGCGTGGCACGTTTGGAAGTGTTTATGTTGCTACCAATAGAGAAACTGGAGCTTTATGTGCAATGAAAGAAGTCGAGTTATTTCCTGAGGACCCAAAATCTGCAGAGTGTATAAAGCAGCTACAGCAGGAAATCAAAGTTCTCAGCCAGCTGAAGCATCCTAATATTGTGCAGTATTATGGAAGCGAAATAGTTGATGATCGATTTTATATATATCTGGAGTTTGTCCATCCTGGTTCTATTGACAAATATGTCCATGAACATTGTGGAGCCATAACAGAAGCTGTTGTTCGCAATTTTACTCGCCATATACTCTCTGGGTTGGCCTACTTGCACAGCACAAAGACAATACACAGGGACATCAAGGGGGCTAATTTGCTTGTTGATTCATATGGAGTTGTCAAGCTAGCTGACTTTGGAATGGCAAAACATCTTTCAGGTCACGTAGGAAATCTTTCTCTAAAGGGAAGCCCATACTGGATGGCTCCAGAGCTGATGCAAGCTGAGATGCATAAAGATAGCAGCTCTGATCTGGCTCTTGCTGTAGATATATGGAGCTTGGGTTGCACAATTATTGAAATGTTCACCGGAAAACCTCCTTGGAGTGAGTATGAAGGGGCTGCGGCCATGTTTAAAGTTATGAAGGATGCACCACCAATTCCAGAAACATTGTCACCTGAGGGTAAGGATTTCCTATGCTGCTGCTTCCAAAGAAATCCCGCAGAGAGGCCAACTGCTGCCATGTTATTAGAACATCGATTCTTAAAAAACTCGCAGCAGCAGGATGTCCCGCCTGGCACTCAGGCATCTAATGGGATGAATTACGCGGATAAATCCCATAGTCCAATCGAACTTGAAAGAAAATTTGATCAGTCGCTGATGATTCCTGCCATAATTGGATCACAGAGCAGTGCATTCCCAATTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

621

Amino Acids

67.72

Weight (kDa)

7.93

Isoelectric Point (pI)

63.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 305 - 566 1.4e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 308 - 562 7.9e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 505
AasI GACNNNNNNGTC 1 cut(s) 1184
Acc36I ACCTGC 1 cut(s) 505
AccB7I CCANNNNNTGG 1 cut(s) 18
AccI GTMKAC 1 cut(s) 410
AccII CGCG 4 cut(s) 284, 370, 787, 1760
AccIII TCCGGA 1 cut(s) 268
AclWI GGATC 1 cut(s) 1840
AcoI YGGCCR 3 cut(s) 285, 358, 1557
AcsI RAATTY 1 cut(s) 1796
AcuI CTGAAG 2 cut(s) 831, 1106
AfaI GTAC 1 cut(s) 193
AfiI CCNNNNNNNGG 8 cut(s) 18, 171, 214, 288, 545, 786, 905, 1659
AgsI TTSAA 5 cut(s) 395, 470, 773, 1511, 1790
AjiI CACGTC 1 cut(s) 279
AjnI CCWGG 5 cut(s) 207, 733, 835, 1165, 1726
AjuI GAANNNNNNNTTGG 2 cut(s) 1516, 1548
Alw21I GWGCWC 1 cut(s) 633
Alw26I GTCTC 2 cut(s) 198, 781
AlwI GGATC 1 cut(s) 1840
AlwNI CAGNNNCTG 3 cut(s) 304, 1217, 1810
Ama87I CYCGRG 2 cut(s) 247, 599
Aor13HI TCCGGA 1 cut(s) 268
AoxI GGCC 5 cut(s) 285, 358, 1257, 1557, 1661
ApoI RAATTY 1 cut(s) 1796
ArsI GACNNNNNNTTYG 2 cut(s) 83, 115
Asp700I GAANNNNTTC 1 cut(s) 1387
AspLEI GCGC 3 cut(s) 370, 494, 787
AspS9I GGNCC 1 cut(s) 1024
AsuC2I CCSGG 2 cut(s) 289, 362
AsuHPI GGTGA 5 cut(s) 154, 560, 852, 1510, 1599
AsuNHI GCTAGC 2 cut(s) 500, 1339
AvaI CYCGRG 2 cut(s) 247, 599
AvaII GGWCC 1 cut(s) 1024
AxyI CCTNAGG 2 cut(s) 1020, 1611
BbsI GAAGAC 2 cut(s) 63, 119
Bbv12I GWGCWC 1 cut(s) 633
BbvCI CCTCAGC 1 cut(s) 297
BccI CCATC 5 cut(s) 330, 703, 825, 1170, 1408
BceAI ACGGC 2 cut(s) 61, 212
BciT130I CCWGG 5 cut(s) 209, 735, 837, 1167, 1728
BclI TGATCA 1 cut(s) 1801
BcnI CCSGG 2 cut(s) 289, 362
BcoDI GTCTC 2 cut(s) 198, 781
BfaI CTAG 2 cut(s) 501, 1340
BfmI CTRYAG 3 cut(s) 1035, 1055, 1476
BfuAI ACCTGC 1 cut(s) 505
Bme1390I CCNGG 7 cut(s) 209, 289, 362, 735, 837, 1167, 1728
Bme18I GGWCC 1 cut(s) 1024
BmeT110I CYCGRG 2 cut(s) 247, 599
BmgBI CACGTC 1 cut(s) 279
BmgT120I GGNCC 1 cut(s) 1024
BmiI GGNNCC 4 cut(s) 226, 1026, 1204, 1419
BmrFI CCNGG 7 cut(s) 209, 289, 362, 735, 837, 1167, 1728
BmrI ACTGGG 1 cut(s) 534
BmsI GCATC 7 cut(s) 71, 594, 1099, 1419, 1431, 1570, 1748
BmtI GCTAGC 2 cut(s) 504, 1343
BmuI ACTGGG 1 cut(s) 534
BpiI GAAGAC 2 cut(s) 63, 119
BpmI CTGGAG 4 cut(s) 858, 1002, 1172, 1404
Bpu10I CCTNAGC 1 cut(s) 297
BpuMI CCSGG 2 cut(s) 289, 362
Bsa29I ATCGAT 2 cut(s) 1135, 1690
BsaAI YACGTR 1 cut(s) 1378
BsaI GGTCTC 1 cut(s) 198
BsaJI CCNNGG 3 cut(s) 12, 282, 1532
BsaWI WCCGGW 4 cut(s) 144, 268, 886, 1520
BsaXI ACNNNNNCTCC 2 cut(s) 1480, 1510
Bsc4I CCNNNNNNNGG 8 cut(s) 18, 171, 214, 288, 545, 786, 905, 1659
Bse1I ACTGG 5 cut(s) 199, 540, 899, 985, 1415
Bse21I CCTNAGG 2 cut(s) 1020, 1611
Bse3DI GCAATG 3 cut(s) 749, 821, 1002
BseAI TCCGGA 1 cut(s) 268
BseBI CCWGG 5 cut(s) 209, 735, 837, 1167, 1728
BseCI ATCGAT 2 cut(s) 1135, 1690
BseDI CCNNGG 3 cut(s) 12, 282, 1532
BseGI GGATG 7 cut(s) 817, 1090, 1162, 1419, 1585, 1723, 1755
BseLI CCNNNNNNNGG 8 cut(s) 18, 171, 214, 288, 545, 786, 905, 1659
BseMI GCAATG 3 cut(s) 749, 821, 1002
BseMII CTCAG 6 cut(s) 311, 1011, 1090, 1428, 1602, 1748
BseNI ACTGG 5 cut(s) 199, 540, 899, 985, 1415
BseRI GAGGAG 4 cut(s) 43, 166, 285, 430
BseX3I CGGCCG 1 cut(s) 285
BseYI CCCAGC 1 cut(s) 894
BsgI GTGCAG 1 cut(s) 1124
Bsh1236I CGCG 4 cut(s) 284, 370, 787, 1760
Bsh1285I CGRYCG 2 cut(s) 149, 288
BshFI GGCC 5 cut(s) 287, 360, 1259, 1559, 1663
BshVI ATCGAT 2 cut(s) 1135, 1690
BsiEI CGRYCG 2 cut(s) 149, 288
BsiHKAI GWGCWC 1 cut(s) 633
BsiHKCI CYCGRG 2 cut(s) 247, 599
BsiSI CCGG 7 cut(s) 145, 269, 288, 314, 361, 887, 1521
BslFI GGGAC 7 cut(s) 304, 522, 581, 693, 877, 1304, 1706
BslI CCNNNNNNNGG 8 cut(s) 18, 171, 214, 288, 545, 786, 905, 1659
BsmAI GTCTC 2 cut(s) 198, 781
BsmFI GGGAC 7 cut(s) 304, 522, 581, 693, 877, 1304, 1706
BsmI GAATGC 1 cut(s) 1847
BsnI GGCC 5 cut(s) 287, 360, 1259, 1559, 1663
Bso31I GGTCTC 1 cut(s) 198
BsoBI CYCGRG 2 cut(s) 247, 599
Bsp1286I GDGCHC 1 cut(s) 633
Bsp13I TCCGGA 1 cut(s) 268
Bsp143I GATC 7 cut(s) 83, 186, 550, 1132, 1462, 1801, 1832
Bsp19I CCATGG 1 cut(s) 12
BspANI GGCC 5 cut(s) 287, 360, 1259, 1559, 1663
BspCNI CTCAG 6 cut(s) 310, 1012, 1089, 1429, 1603, 1747
BspDI ATCGAT 2 cut(s) 1135, 1690
BspEI TCCGGA 1 cut(s) 268
BspFNI CGCG 4 cut(s) 284, 370, 787, 1760
BspLI GGNNCC 4 cut(s) 226, 1026, 1204, 1419
BspMAI CTGCAG 1 cut(s) 1039
BspMI ACCTGC 1 cut(s) 505
BspOI GCTAGC 2 cut(s) 504, 1343
BspPI GGATC 1 cut(s) 1840
BspTNI GGTCTC 1 cut(s) 198
BsrDI GCAATG 3 cut(s) 749, 821, 1002
BsrI ACTGG 5 cut(s) 199, 540, 899, 985, 1415
BssECI CCNNGG 3 cut(s) 12, 282, 1532
BssMI GATC 7 cut(s) 83, 186, 550, 1132, 1462, 1801, 1832
BssT1I CCWWGG 2 cut(s) 12, 1532
Bst2UI CCWGG 5 cut(s) 209, 735, 837, 1167, 1728
Bst4CI ACNGT 1 cut(s) 536
Bst6I CTCTTC 1 cut(s) 46
BstBAI YACGTR 1 cut(s) 1378
BstC8I GCNNGC 4 cut(s) 502, 1082, 1341, 1434
BstDEI CTNAG 7 cut(s) 297, 848, 1020, 1076, 1437, 1611, 1734
BstDSI CCRYGG 2 cut(s) 12, 282
BstF5I GGATG 7 cut(s) 817, 1090, 1162, 1419, 1585, 1723, 1755
BstFNI CGCG 4 cut(s) 284, 370, 787, 1760
BstHHI GCGC 3 cut(s) 370, 494, 787
BstKTI GATC 7 cut(s) 86, 189, 553, 1135, 1465, 1804, 1835
BstMAI GTCTC 2 cut(s) 198, 781
BstMBI GATC 7 cut(s) 83, 186, 550, 1132, 1462, 1801, 1832
BstMCI CGRYCG 2 cut(s) 149, 288
BstMWI GCNNNNNNNGC 5 cut(s) 310, 934, 992, 1057, 1497
BstNI CCWGG 5 cut(s) 209, 735, 837, 1167, 1728
BstSCI CCNGG 7 cut(s) 207, 287, 360, 733, 835, 1165, 1726
BstSFI CTRYAG 3 cut(s) 1035, 1055, 1476
BstUI CGCG 4 cut(s) 284, 370, 787, 1760
BstV2I GAAGAC 2 cut(s) 63, 119
BstZI CGGCCG 1 cut(s) 285
Bsu15I ATCGAT 2 cut(s) 1135, 1690
Bsu36I CCTNAGG 2 cut(s) 1020, 1611
BsuRI GGCC 5 cut(s) 287, 360, 1259, 1559, 1663
BsuTUI ATCGAT 2 cut(s) 1135, 1690
BtgI CCRYGG 2 cut(s) 12, 282
BtgZI GCGATG 2 cut(s) 135, 729
BtrI CACGTC 1 cut(s) 279
BtsCI GGATG 7 cut(s) 817, 1090, 1162, 1419, 1585, 1723, 1755
BtsI GCAGTG 2 cut(s) 780, 1849
BtsIMutI CAGTG 4 cut(s) 547, 780, 906, 1849
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 4 cut(s) 502, 1082, 1341, 1434
CaiI CAGNNNCTG 3 cut(s) 304, 1217, 1810
CfoI GCGC 3 cut(s) 370, 494, 787
Cfr13I GGNCC 1 cut(s) 1024
Cfr42I CCGCGG 1 cut(s) 285
ClaI ATCGAT 2 cut(s) 1135, 1690
Csp6I GTAC 1 cut(s) 192
CviAII CATG 4 cut(s) 13, 1190, 1561, 1675
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 7 cut(s) 297, 848, 1020, 1076, 1437, 1611, 1734
DpnI GATC 7 cut(s) 85, 188, 552, 1134, 1464, 1803, 1834
DpnII GATC 7 cut(s) 83, 186, 550, 1132, 1462, 1801, 1832
DraI TTTAAA 1 cut(s) 1567
DrdI GACNNNNNNGTC 1 cut(s) 1184
DseDI GACNNNNNNGTC 1 cut(s) 1184
EaeI YGGCCR 3 cut(s) 285, 358, 1557
EagI CGGCCG 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 46
EarI CTCTTC 1 cut(s) 46
EciI GGCGGA 1 cut(s) 216
EclXI CGGCCG 1 cut(s) 285
Eco130I CCWWGG 2 cut(s) 12, 1532
Eco31I GGTCTC 1 cut(s) 198
Eco47I GGWCC 1 cut(s) 1024
Eco52I CGGCCG 1 cut(s) 285
Eco57I CTGAAG 2 cut(s) 831, 1106
Eco81I CCTNAGG 2 cut(s) 1020, 1611
Eco88I CYCGRG 2 cut(s) 247, 599
EcoO109I RGGNCCY 1 cut(s) 1024
EcoRII CCWGG 5 cut(s) 207, 733, 835, 1165, 1726
EcoT14I CCWWGG 2 cut(s) 12, 1532
EcoT22I ATGCAT 1 cut(s) 1446
ErhI CCWWGG 2 cut(s) 12, 1532
FaeI CATG 4 cut(s) 16, 1193, 1564, 1678
FaqI GGGAC 7 cut(s) 304, 522, 581, 693, 877, 1304, 1706
FatI CATG 4 cut(s) 12, 1189, 1560, 1674
FauI CCCGC 2 cut(s) 1660, 1731
FauNDI CATATG 1 cut(s) 1324
FbaI TGATCA 1 cut(s) 1801
FblI GTMKAC 1 cut(s) 410
FokI GGATG 7 cut(s) 804, 1077, 1149, 1426, 1592, 1730, 1762
FspBI CTAG 2 cut(s) 501, 1340
GlaI GCGC 3 cut(s) 369, 493, 786
GsaI CCCAGC 1 cut(s) 898
GsuI CTGGAG 4 cut(s) 858, 1002, 1172, 1404
HaeIII GGCC 5 cut(s) 287, 360, 1259, 1559, 1663
HapII CCGG 7 cut(s) 145, 269, 288, 314, 361, 887, 1521
HhaI GCGC 3 cut(s) 370, 494, 787
Hin1II CATG 4 cut(s) 16, 1193, 1564, 1678
Hin6I GCGC 3 cut(s) 368, 492, 785
HinP1I GCGC 3 cut(s) 368, 492, 785
HindIII AAGCTT 1 cut(s) 926
HpaII CCGG 7 cut(s) 145, 269, 288, 314, 361, 887, 1521
HphI GGTGA 5 cut(s) 154, 560, 852, 1510, 1599
Hpy166II GTNNAC 2 cut(s) 411, 1518
Hpy188I TCNGA 3 cut(s) 83, 652, 1462
Hpy188III TCNNGA 6 cut(s) 269, 333, 1019, 1151, 1421, 1595
Hpy8I GTNNAC 2 cut(s) 411, 1518
Hpy99I CGWCG 3 cut(s) 61, 70, 265
HpyAV CCTTC 5 cut(s) 46, 651, 867, 1541, 1570
HpyCH4III ACNGT 1 cut(s) 536
HpyCH4IV ACGT 3 cut(s) 278, 944, 1377
HpyF10VI GCNNNNNNNGC 5 cut(s) 310, 934, 992, 1057, 1497
HpyF3I CTNAG 7 cut(s) 297, 848, 1020, 1076, 1437, 1611, 1734
HpySE526I ACGT 3 cut(s) 278, 944, 1377
Hsp92II CATG 4 cut(s) 16, 1193, 1564, 1678
HspAI GCGC 3 cut(s) 368, 492, 785
Kpn2I TCCGGA 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 1801
KspI CCGCGG 1 cut(s) 285
Kzo9I GATC 7 cut(s) 83, 186, 550, 1132, 1462, 1801, 1832
LmnI GCTCC 8 cut(s) 224, 489, 636, 839, 983, 1202, 1423, 1488
LweI GCATC 7 cut(s) 71, 594, 1099, 1419, 1431, 1570, 1748
MaeI CTAG 2 cut(s) 501, 1340
MaeII ACGT 3 cut(s) 278, 944, 1377
MaeIII GTNAC 2 cut(s) 1373, 1605
MalI GATC 7 cut(s) 85, 188, 552, 1134, 1464, 1803, 1834
MboI GATC 7 cut(s) 83, 186, 550, 1132, 1462, 1801, 1832
MboII GAAGA 6 cut(s) 33, 36, 39, 63, 124, 545
MfeI CAATTG 1 cut(s) 768
MhlI GDGCHC 1 cut(s) 633
MlyI GAGTC 4 cut(s) 107, 286, 523, 899
MmeI TCCRAC 1 cut(s) 630
Mph1103I ATGCAT 1 cut(s) 1446
MroI TCCGGA 1 cut(s) 268
MroXI GAANNNNTTC 1 cut(s) 1387
MseI TTAA 2 cut(s) 1566, 1697
MspA1I CMGCKG 4 cut(s) 284, 304, 326, 1084
MspI CCGG 7 cut(s) 145, 269, 288, 314, 361, 887, 1521
MspR9I CCNGG 7 cut(s) 209, 289, 362, 735, 837, 1167, 1728
MunI CAATTG 1 cut(s) 768
Mva1269I GAATGC 1 cut(s) 1847
MvaI CCWGG 5 cut(s) 209, 735, 837, 1167, 1728
MvnI CGCG 4 cut(s) 284, 370, 787, 1760
MwoI GCNNNNNNNGC 5 cut(s) 310, 934, 992, 1057, 1497
NciI CCSGG 2 cut(s) 289, 362
NcoI CCATGG 1 cut(s) 12
NdeI CATATG 1 cut(s) 1324
NdeII GATC 7 cut(s) 83, 186, 550, 1132, 1462, 1801, 1832
NheI GCTAGC 2 cut(s) 500, 1339
NlaIII CATG 4 cut(s) 16, 1193, 1564, 1678
NlaIV GGNNCC 4 cut(s) 226, 1026, 1204, 1419
NmuCI GTSAC 2 cut(s) 1373, 1605
NsiI ATGCAT 1 cut(s) 1446
PaqCI CACCTGC 1 cut(s) 505
PcsI WCGNNNNNNNCGW 3 cut(s) 62, 65, 74
PctI GAATGC 1 cut(s) 1847
PdmI GAANNNNTTC 1 cut(s) 1387
PfeI GAWTC 6 cut(s) 329, 395, 421, 1319, 1692, 1816
PflMI CCANNNNNTGG 1 cut(s) 18
PfoI TCCNGGA 1 cut(s) 835
PleI GAGTC 4 cut(s) 106, 286, 522, 898
PpsI GAGTC 4 cut(s) 106, 286, 522, 898
Ppu21I YACGTR 1 cut(s) 1378
PpuMI RGGWCCY 1 cut(s) 1024
Psp5II RGGWCCY 1 cut(s) 1024
Psp6I CCWGG 5 cut(s) 207, 733, 835, 1165, 1726
PspFI CCCAGC 1 cut(s) 894
PspGI CCWGG 5 cut(s) 207, 733, 835, 1165, 1726
PspN4I GGNNCC 4 cut(s) 226, 1026, 1204, 1419
PspPI GGNCC 1 cut(s) 1024
PspPPI RGGWCCY 1 cut(s) 1024
PstI CTGCAG 1 cut(s) 1039
PstNI CAGNNNCTG 3 cut(s) 304, 1217, 1810
PvuII CAGCTG 1 cut(s) 1084
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
SacII CCGCGG 1 cut(s) 285
SaqAI TTAA 2 cut(s) 1566, 1697
Sau3AI GATC 7 cut(s) 83, 186, 550, 1132, 1462, 1801, 1832
Sau96I GGNCC 1 cut(s) 1024
SchI GAGTC 4 cut(s) 107, 286, 523, 899
ScrFI CCNGG 7 cut(s) 209, 289, 362, 735, 837, 1167, 1728
SduI GDGCHC 1 cut(s) 633
SfaNI GCATC 7 cut(s) 71, 594, 1099, 1419, 1431, 1570, 1748
SfcI CTRYAG 3 cut(s) 1035, 1055, 1476
Sfr303I CCGCGG 1 cut(s) 285
SgrBI CCGCGG 1 cut(s) 285
SinI GGWCC 1 cut(s) 1024
SspI AATATT 1 cut(s) 1099
SspMI CTAG 2 cut(s) 501, 1340
StyD4I CCNGG 7 cut(s) 207, 287, 360, 733, 835, 1165, 1726
StyI CCWWGG 2 cut(s) 12, 1532
TaaI ACNGT 1 cut(s) 536
TaiI ACGT 3 cut(s) 281, 947, 1380
TaqI TCGA 7 cut(s) 149, 254, 512, 1008, 1135, 1690, 1782
TatI WGTACW 1 cut(s) 191
TauI GCSGC 5 cut(s) 201, 287, 304, 790, 1559
TfiI GAWTC 6 cut(s) 329, 395, 421, 1319, 1692, 1816
Tru1I TTAA 2 cut(s) 1566, 1697
Tru9I TTAA 2 cut(s) 1566, 1697
TscAI CASTG 4 cut(s) 547, 787, 906, 1849
TseFI GTSAC 2 cut(s) 1373, 1605
Tsp45I GTSAC 2 cut(s) 1373, 1605
TspDTI ATGAA 6 cut(s) 1013, 1206, 1311, 1560, 1589, 1766
TspRI CASTG 4 cut(s) 547, 787, 906, 1849
Van91I CCANNNNNTGG 1 cut(s) 18
VpaK11BI GGWCC 1 cut(s) 1024
XapI RAATTY 1 cut(s) 1796
XcmI CCANNNNNNNNNTGG 3 cut(s) 483, 1196, 1247
XmiI GTMKAC 1 cut(s) 410
XmnI GAANNNNTTC 1 cut(s) 1387
XspI CTAG 2 cut(s) 501, 1340
Zsp2I ATGCAT 1 cut(s) 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.