Rh2AG173200

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001271
Physical Location & Seq
Forward (+)
16501969 .. 16512895
10927 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG173200.1

Sequence Viewer

Length: 765 bp
ATGACAAGGAGTCGCCATGTGTTTTCTTGGCGAGACACTCCAAAGAATAACATTCAAGTACGTGAGACAGTGTCGTCTAGGAGGAAGAATCGAGATCGAAATCATTTACAGATCCCTGAACATGACTTCAGGCTCGATGTTCCTGTTAGAAGTGCACCCACTAGTCCATGCGCAAGTCCTAGATTCAGCCCACGCAGACCAAGTGCTGCTGATTTGTGGGCTCCGTCCAGAAATCAAGTCTGGTCTGCACCGGAGATGACTACTAATGATATGACTCAACCTCCCCCTGCCTTATCAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTTCCACAGGTAGAACTCCCCTTCGACCCCCACAAGGATCATCCCCATTGCATCCCCGGTTATCTGTTACCTCTGAAGTCCATCCATTGCCTCTGCCCCCTGGAGCAAGCCTCCCTTCACCTAGTGTACCATCACCACAACCTGCACTCACACCTAAGCCAGAGTCCCAGCCGATTAGAGGGCAATGGCAAAAGGGAAAGCTTATTGGTCGTGGCACGTTTGGCAGTGTTTATGTTGCTACAAATAGGTACATGCAGTTCCTTGTACAGGAAATCAAAGTCCTCAGCCAGCTCAAGCATCCTAATATTGTGCTGTACTATGGAAGTGAAATTGTGAGTAACGTCTGCTTTTATGTTAAGCATCCAGGCTCATGTTCTTCCATTGGACTACGCCTGAAACAAAACACAAAATACTTTCATGTATATGCAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

254

Amino Acids

28.35

Weight (kDa)

10.34

Isoelectric Point (pI)

67.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 73
Acc16I TGCGCA 1 cut(s) 172
Acc36I ACCTGC 2 cut(s) 481, 749
AclWI GGATC 2 cut(s) 106, 376
AcuI CTGAAG 2 cut(s) 112, 426
AdeI CACNNNGTG 1 cut(s) 455
AfaI GTAC 5 cut(s) 60, 459, 581, 597, 647
AfiI CCNNNNNNNGG 3 cut(s) 365, 509, 598
AgsI TTSAA 1 cut(s) 56
AhdI GACNNNNNGTC 1 cut(s) 9
AhlI ACTAGT 1 cut(s) 161
AjnI CCWGG 2 cut(s) 430, 694
AluBI AGCT 2 cut(s) 532, 622
AluI AGCT 2 cut(s) 532, 622
Alw21I GWGCWC 1 cut(s) 157
Alw26I GTCTC 2 cut(s) 27, 59
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 2 cut(s) 106, 376
ApaLI GTGCAC 1 cut(s) 153
ApeKI GCWGC 1 cut(s) 206
AspLEI GCGC 1 cut(s) 173
AsuC2I CCSGG 1 cut(s) 388
AsuHPI GGTGA 2 cut(s) 441, 456
BaeGI GKGCMC 1 cut(s) 157
BanII GRGCYC 1 cut(s) 223
Bbv12I GWGCWC 1 cut(s) 157
BbvCI CCTCAGC 1 cut(s) 614
BbvI GCAGC 1 cut(s) 193
BccI CCATC 2 cut(s) 420, 469
BciT130I CCWGG 2 cut(s) 432, 696
BcnI CCSGG 1 cut(s) 388
BcoDI GTCTC 2 cut(s) 27, 59
BcuI ACTAGT 1 cut(s) 161
BfaI CTAG 4 cut(s) 78, 162, 180, 453
BfuAI ACCTGC 2 cut(s) 481, 749
BisI GCNGC 1 cut(s) 207
BlsI GCNGC 1 cut(s) 208
Bme1390I CCNGG 3 cut(s) 388, 432, 696
BmeRI GACNNNNNGTC 1 cut(s) 9
BmiI GGNNCC 1 cut(s) 222
BmrFI CCNGG 3 cut(s) 388, 432, 696
BmsI GCATC 3 cut(s) 391, 637, 700
BplI GAGNNNNNCTC 2 cut(s) 426, 458
BpmI CTGGAG 1 cut(s) 453
Bpu10I CCTNAGC 2 cut(s) 486, 614
BpuEI CTTGAG 1 cut(s) 608
BpuMI CCSGG 1 cut(s) 388
BsaAI YACGTR 1 cut(s) 62
BsaBI GATNNNNATC 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 386, 430
BsaWI WCCGGW 1 cut(s) 250
BsaXI ACNNNNNCTCC 2 cut(s) 265, 295
Bsc4I CCNNNNNNNGG 3 cut(s) 365, 509, 598
Bse3DI GCAATG 3 cut(s) 377, 416, 521
Bse8I GATNNNNATC 1 cut(s) 99
BseBI CCWGG 2 cut(s) 432, 696
BseDI CCNNGG 2 cut(s) 386, 430
BseGI GGATG 5 cut(s) 371, 382, 412, 628, 691
BseJI GATNNNNATC 1 cut(s) 99
BseLI CCNNNNNNNGG 3 cut(s) 365, 509, 598
BseMI GCAATG 3 cut(s) 377, 416, 521
BseMII CTCAG 1 cut(s) 628
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 1 cut(s) 193
BseYI CCCAGC 1 cut(s) 498
BsgI GTGCAG 2 cut(s) 231, 459
BsiHKAI GWGCWC 1 cut(s) 157
BsiSI CCGG 2 cut(s) 251, 388
BslFI GGGAC 1 cut(s) 481
BslI CCNNNNNNNGG 3 cut(s) 365, 509, 598
BsmAI GTCTC 2 cut(s) 27, 59
BsmFI GGGAC 1 cut(s) 481
Bsp1286I GDGCHC 2 cut(s) 157, 223
Bsp1407I TGTACA 1 cut(s) 595
Bsp143I GATC 3 cut(s) 94, 111, 368
BspCNI CTCAG 1 cut(s) 627
BspLI GGNNCC 1 cut(s) 222
BspMI ACCTGC 2 cut(s) 481, 749
BspPI GGATC 2 cut(s) 106, 376
BsrDI GCAATG 3 cut(s) 377, 416, 521
BsrGI TGTACA 1 cut(s) 595
BssECI CCNNGG 2 cut(s) 386, 430
BssMI GATC 3 cut(s) 94, 111, 368
Bst2UI CCWGG 2 cut(s) 432, 696
Bst4CI ACNGT 2 cut(s) 70, 332
BstAUI TGTACA 1 cut(s) 595
BstBAI YACGTR 1 cut(s) 62
BstC8I GCNNGC 2 cut(s) 439, 620
BstDEI CTNAG 2 cut(s) 486, 614
BstENI CCTNNNNNAGG 1 cut(s) 596
BstF5I GGATG 5 cut(s) 371, 382, 412, 628, 691
BstHHI GCGC 1 cut(s) 173
BstKTI GATC 3 cut(s) 97, 114, 371
BstMAI GTCTC 2 cut(s) 27, 59
BstMBI GATC 3 cut(s) 94, 111, 368
BstMWI GCNNNNNNNGC 1 cut(s) 552
BstNI CCWGG 2 cut(s) 432, 696
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 3 cut(s) 386, 430, 694
BstSLI GKGCMC 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 111
BstYI RGATCY 1 cut(s) 111
BtsCI GGATG 5 cut(s) 371, 382, 412, 628, 691
BtsI GCAGTG 1 cut(s) 562
BtsIMutI CAGTG 2 cut(s) 75, 562
BveI ACCTGC 2 cut(s) 481, 749
Cac8I GCNNGC 2 cut(s) 439, 620
CfoI GCGC 1 cut(s) 173
Csp6I GTAC 5 cut(s) 59, 458, 580, 596, 646
CviAII CATG 6 cut(s) 17, 122, 168, 583, 702, 749
CviQI GTAC 5 cut(s) 59, 458, 580, 596, 646
DdeI CTNAG 2 cut(s) 486, 614
DpnI GATC 3 cut(s) 96, 113, 370
DpnII GATC 3 cut(s) 94, 111, 368
DraIII CACNNNGTG 1 cut(s) 455
DrdI GACNNNNNNGTC 1 cut(s) 73
DriI GACNNNNNGTC 1 cut(s) 9
DseDI GACNNNNNNGTC 1 cut(s) 73
Eam1105I GACNNNNNGTC 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 223
Eco57I CTGAAG 2 cut(s) 112, 426
EcoNI CCTNNNNNAGG 1 cut(s) 596
EcoRII CCWGG 2 cut(s) 430, 694
EcoT38I GRGCYC 1 cut(s) 223
FaeI CATG 6 cut(s) 20, 125, 171, 586, 705, 752
FalI AAGNNNNNCTT 2 cut(s) 430, 462
FaqI GGGAC 1 cut(s) 481
FatI CATG 6 cut(s) 16, 121, 167, 582, 701, 748
Fnu4HI GCNGC 1 cut(s) 207
FokI GGATG 5 cut(s) 358, 369, 399, 615, 678
FriOI GRGCYC 1 cut(s) 223
Fsp4HI GCNGC 1 cut(s) 207
FspBI CTAG 4 cut(s) 78, 162, 180, 453
FspI TGCGCA 1 cut(s) 172
GlaI GCGC 1 cut(s) 172
GluI GCNGC 1 cut(s) 207
GsaI CCCAGC 1 cut(s) 502
GsuI CTGGAG 1 cut(s) 453
HapII CCGG 2 cut(s) 251, 388
HhaI GCGC 1 cut(s) 173
Hin1II CATG 6 cut(s) 20, 125, 171, 586, 705, 752
Hin6I GCGC 1 cut(s) 171
HinP1I GCGC 1 cut(s) 171
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 5 cut(s) 10, 88, 183, 274, 494
HpaII CCGG 2 cut(s) 251, 388
HphI GGTGA 2 cut(s) 441, 456
Hpy166II GTNNAC 2 cut(s) 155, 458
Hpy188I TCNGA 2 cut(s) 298, 406
Hpy188III TCNNGA 2 cut(s) 92, 228
Hpy8I GTNNAC 2 cut(s) 155, 458
HpyAV CCTTC 2 cut(s) 362, 456
HpyCH4III ACNGT 2 cut(s) 70, 332
HpyCH4IV ACGT 3 cut(s) 61, 548, 672
HpyCH4V TGCA 6 cut(s) 155, 248, 382, 476, 586, 758
HpyF10VI GCNNNNNNNGC 1 cut(s) 552
HpyF3I CTNAG 2 cut(s) 486, 614
HpySE526I ACGT 3 cut(s) 61, 548, 672
Hsp92II CATG 6 cut(s) 20, 125, 171, 586, 705, 752
HspAI GCGC 1 cut(s) 171
Kzo9I GATC 3 cut(s) 94, 111, 368
LmnI GCTCC 2 cut(s) 226, 434
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 3 cut(s) 391, 637, 700
MaeI CTAG 4 cut(s) 78, 162, 180, 453
MaeII ACGT 3 cut(s) 61, 548, 672
MaeIII GTNAC 2 cut(s) 397, 668
MalI GATC 3 cut(s) 96, 113, 370
MboI GATC 3 cut(s) 94, 111, 368
MboII GAAGA 2 cut(s) 97, 699
MflI RGATCY 1 cut(s) 111
MhlI GDGCHC 2 cut(s) 157, 223
MluCI AATT 1 cut(s) 660
MlyI GAGTC 3 cut(s) 19, 268, 503
MnlI CCTC 7 cut(s) 75, 291, 412, 432, 452, 503, 623
MseI TTAA 1 cut(s) 687
MslI CAYNNNNRTG 1 cut(s) 753
MspI CCGG 2 cut(s) 251, 388
MspR9I CCNGG 3 cut(s) 388, 432, 696
MvaI CCWGG 2 cut(s) 432, 696
MwoI GCNNNNNNNGC 1 cut(s) 552
NciI CCSGG 1 cut(s) 388
NdeII GATC 3 cut(s) 94, 111, 368
NlaIII CATG 6 cut(s) 20, 125, 171, 586, 705, 752
NlaIV GGNNCC 1 cut(s) 222
NsbI TGCGCA 1 cut(s) 172
NspI RCATGY 1 cut(s) 586
PfeI GAWTC 2 cut(s) 88, 183
PflFI GACNNNGTC 1 cut(s) 70
PkrI GCNGC 1 cut(s) 208
PleI GAGTC 3 cut(s) 18, 268, 502
PpsI GAGTC 3 cut(s) 18, 268, 502
Ppu21I YACGTR 1 cut(s) 62
Psp6I CCWGG 2 cut(s) 430, 694
PspFI CCCAGC 1 cut(s) 498
PspGI CCWGG 2 cut(s) 430, 694
PspN4I GGNNCC 1 cut(s) 222
PsrI GAACNNNNNNTAC 4 cut(s) 301, 333, 572, 604
PsuI RGATCY 1 cut(s) 111
PsyI GACNNNGTC 1 cut(s) 70
RsaI GTAC 5 cut(s) 60, 459, 581, 597, 647
RsaNI GTAC 5 cut(s) 59, 458, 580, 596, 646
RseI CAYNNNNRTG 1 cut(s) 753
SaqAI TTAA 1 cut(s) 687
SatI GCNGC 1 cut(s) 207
Sau3AI GATC 3 cut(s) 94, 111, 368
SchI GAGTC 3 cut(s) 19, 268, 503
ScrFI CCNGG 3 cut(s) 388, 432, 696
SduI GDGCHC 2 cut(s) 157, 223
SfaNI GCATC 3 cut(s) 391, 637, 700
SmiMI CAYNNNNRTG 1 cut(s) 753
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
SpeI ACTAGT 1 cut(s) 161
Sse9I AATT 1 cut(s) 660
SspI AATATT 1 cut(s) 637
SspMI CTAG 4 cut(s) 78, 162, 180, 453
StyD4I CCNGG 3 cut(s) 386, 430, 694
TaaI ACNGT 2 cut(s) 70, 332
TaiI ACGT 3 cut(s) 64, 551, 675
TaqI TCGA 4 cut(s) 91, 97, 135, 355
TasI AATT 1 cut(s) 660
TatI WGTACW 2 cut(s) 595, 645
TfiI GAWTC 2 cut(s) 88, 183
Tru1I TTAA 1 cut(s) 687
Tru9I TTAA 1 cut(s) 687
TscAI CASTG 2 cut(s) 75, 562
TseI GCWGC 1 cut(s) 206
TspDTI ATGAA 2 cut(s) 327, 737
TspGWI ACGGA 1 cut(s) 213
TspRI CASTG 2 cut(s) 75, 562
Tth111I GACNNNGTC 1 cut(s) 70
VneI GTGCAC 1 cut(s) 153
XagI CCTNNNNNAGG 1 cut(s) 596
XceI RCATGY 1 cut(s) 586
XspI CTAG 4 cut(s) 78, 162, 180, 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.