Rw2G013520

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001271
Physical Location & Seq
Forward (+)
14546238 .. 14549733
3496 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013520.1

Sequence Viewer

Length: 1830 bp
ATGCTAGCCACAACCATGCGTTGGTTGCCTAGCTTTGCTTCCTCATCATCATCTGCTTCGTCCTCGTCGTCGTCGTCGCCGAAAAAGAAGGCCAGGGAGGACGGCGGGTGGATTTTCCGGTCGAGGAGGAAGGCCGCGCAGTCGAGGAAACTGCTCAATTTGGGGAGAGACAATGACGGTGGAGTTATAACCAAATGCTCCAGCGCGCCGGGGTCGGAATCCGCGCTGGCCAGTCCGCAGCCGCTTCCTTTGCCGGAATCGGCCATCCGCCGAGAGACGAGGTGTTCTCGCTGTAAGTCATGGGGACATGAGAAATGCTTCCCCTCCCCGGCTCCCAGTGTGTTTTCTTGGCGAGACACTCCAAAGAATAAGGTTCAAGTACGTGAGACAGTGTCGTCTAGGAGGAAGAATCATGATCGAAATCGTTTACAGATCCCTGAACATGACTTCAGGCTCGATGTTCCTGTTAGAAGTGCACCCACCAGTCCATGCGCAAGTCCTAGATTCAGCCCACGCAGACCAAGTGCTGCTGATTTGTGGGCTCCGTCCAGAAATCAAGTCTGGTCTGCACCGGAGATGACTACTAATGATATGACTCAACCTCCCCCTGCCTTATCAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTCCCACGGCTAGAAGTCCCCTTCGACCCCCACAAGCATCATCCCCATTGCATCCCCGGTTATCTGTTACCTCTGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGCCTCCCTTCACCTAGTGTACCATCACCACAACCTGCACTCACACCTAAGCCAGAGTCCCAGCCGATTAAAGGGCAATGGCAAAAGGGAAAGCTTATTGGTCGTGGCACCTTTGGCAGTGTTTATGTTGCTACTAATAGAGAAACTGGAGCTTTATGTGCAATGAAGGAAGTTGAACTACTTCCTGATGACCCGAAATCTGCAGAGTGTATAAAGCAGCTACAGCAGGTTGTCACTGTGATTGTTACTCATACCGTCTTTAATCAGGAAATCAAAGTCCTCAGCCAGCTCAAGCATCCTAATATTGTGCAGTACTATGGAAGTGAAATTGTTGAAGATCGGTTTTTTATATATCTGGAATATGTTCATCCTGGTTCTATTAACAAATATGTTCATGAACACTGTGGAGCCATGACAGAAGCTGTTGTTCGCAGTTTTACTCGCCATATAGTCTCTGGGTTGGCTTACTTGCACAGCACAAAAACAATACACAGGGACATCAAAGGGGCTAATTTGCTTGTGGATTCATGTGGGGTTGTCAAGCTAGCTGACTTTGGAATGGCCAAACATCTTTCAGGACACGTAGGGAATCTTTCCCTAAAGGGAAGTCCATACTGGATGGCTCCAGAGCTGATGCAATCTGAGATGCATAAAGATAACAGCTCTGATTTGGCTCTTGCTGTTGATATATGGAGTTTGGGTTGTACTATTATTGAAATGTTCACAGGAAAACCTCCATGGAGTGAGTATGAAGGGGCTGCAGCTATGTTTAAAGTTATGAAGGATACTCCACCTTTTCCAGAAACATTGTCACCTGAGGGTAAGGACTTCCTTAGGTGCTGCTTCCAAAGAAATCCTGCTGAGAGGTCAACTGCTGCAATGCTATTAGAACATCGATTTATAAAAAACTCCCAGCTGCAGGAAGTCCCATCTACGACCTATAATGCCTCTAACGGGATGAATTTCTTGGAAAGACCCCAGACTCCAATTGAACTTGAAAGAAGACTTGATAGTTCACTGATGATTCCCAGCATTGGCATGCAATTAACTACGGGAAAGGGGCCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

609

Amino Acids

67.19

Weight (kDa)

9.29

Isoelectric Point (pI)

62.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 280 - 554 5.6e-64 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 283 - 550 1.1e-42 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 399 - 504 1e-05 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 188, 1661
AasI GACNNNNNNGTC 1 cut(s) 394
Acc16I TGCGCA 1 cut(s) 493
Acc36I ACCTGC 2 cut(s) 802, 976
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 21
AccII CGCG 3 cut(s) 137, 206, 224
AciI CCGC 6 cut(s) 105, 135, 222, 236, 242, 268
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 3 cut(s) 228, 261, 1320
AcsI RAATTY 1 cut(s) 1720
AcuI CTGAAG 2 cut(s) 433, 747
AdeI CACNNNGTG 1 cut(s) 776
AfaI GTAC 4 cut(s) 381, 780, 1073, 1465
AfiI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1678, 1713, 1793
AflIII ACRYGT 1 cut(s) 1339
AgsI TTSAA 6 cut(s) 377, 935, 1094, 1475, 1751, 1757
AjnI CCWGG 3 cut(s) 92, 751, 1129
Alw21I GWGCWC 1 cut(s) 478
Alw26I GTCTC 5 cut(s) 162, 269, 348, 380, 1216
Alw44I GTGCAC 1 cut(s) 474
AlwI GGATC 1 cut(s) 427
AlwNI CAGNNNCTG 1 cut(s) 1181
AoxI GGCC 6 cut(s) 90, 132, 228, 261, 1320, 1820
ApaLI GTGCAC 1 cut(s) 474
ApeKI GCWGC 8 cut(s) 238, 527, 976, 1517, 1520, 1599, 1634, 1675
ApoI RAATTY 1 cut(s) 1720
Asp700I GAANNNNTTC 3 cut(s) 317, 939, 1122
AspLEI GCGC 5 cut(s) 139, 206, 208, 226, 494
AspS9I GGNCC 1 cut(s) 1820
AsuC2I CCSGG 3 cut(s) 210, 329, 709
AsuHPI GGTGA 3 cut(s) 762, 777, 1563
AsuNHI GCTAGC 2 cut(s) 4, 1303
AxyI CCTNAGG 2 cut(s) 1575, 1592
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 2 cut(s) 230, 1322
BanI GGYRCC 1 cut(s) 866
BanII GRGCYC 1 cut(s) 544
BarI GAAGNNNNNNTAC 2 cut(s) 921, 953
BbsI GAAGAC 1 cut(s) 1768
Bbv12I GWGCWC 1 cut(s) 478
BbvCI CCTCAGC 1 cut(s) 1040
BbvI GCAGC 8 cut(s) 250, 514, 988, 1504, 1532, 1586, 1621, 1662
BccI CCATC 5 cut(s) 272, 741, 790, 1372, 1696
BceAI ACGGC 2 cut(s) 118, 675
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 3 cut(s) 94, 753, 1131
BciVI GTATCC 1 cut(s) 1537
BcnI CCSGG 3 cut(s) 210, 329, 709
BcoDI GTCTC 5 cut(s) 162, 269, 348, 380, 1216
BfaI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1304
BfmI CTRYAG 4 cut(s) 960, 980, 1518, 1676
BfuAI ACCTGC 2 cut(s) 802, 976
BfuI GTATCC 1 cut(s) 1537
BmcAI AGTACT 1 cut(s) 1073
Bme1390I CCNGG 6 cut(s) 94, 210, 329, 709, 753, 1131
BmgT120I GGNCC 1 cut(s) 1820
BmiI GGNNCC 6 cut(s) 333, 543, 868, 1168, 1383, 1821
BmrFI CCNGG 6 cut(s) 94, 210, 329, 709, 753, 1131
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 5 cut(s) 698, 712, 1063, 1383, 1395
BmtI GCTAGC 2 cut(s) 8, 1307
BmuI ACTGGG 1 cut(s) 330
BpiI GAAGAC 1 cut(s) 1768
BplI GAGNNNNNCTC 4 cut(s) 271, 303, 747, 779
BpmI CTGGAG 4 cut(s) 184, 774, 927, 1368
Bpu10I CCTNAGC 2 cut(s) 807, 1040
BpuEI CTTGAG 1 cut(s) 1034
BpuMI CCSGG 3 cut(s) 210, 329, 709
Bsa29I ATCGAT 1 cut(s) 1654
BsaAI YACGTR 2 cut(s) 383, 1342
BsaBI GATNNNNATC 1 cut(s) 420
BsaJI CCNNGG 6 cut(s) 93, 209, 327, 657, 707, 1496
BsaWI WCCGGW 2 cut(s) 117, 571
BsaXI ACNNNNNCTCC 4 cut(s) 586, 616, 1444, 1474
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1678, 1713, 1793
Bse1I ACTGG 5 cut(s) 231, 336, 483, 910, 1379
Bse21I CCTNAGG 2 cut(s) 1575, 1592
Bse3DI GCAATG 5 cut(s) 698, 737, 842, 927, 1644
Bse8I GATNNNNATC 1 cut(s) 420
BseBI CCWGG 3 cut(s) 94, 753, 1131
BseCI ATCGAT 1 cut(s) 1654
BseDI CCNNGG 6 cut(s) 93, 209, 327, 657, 707, 1496
BseGI GGATG 8 cut(s) 264, 692, 703, 733, 1054, 1126, 1383, 1722
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1678, 1713, 1793
BseMI GCAATG 5 cut(s) 698, 737, 842, 927, 1644
BseMII CTCAG 4 cut(s) 1054, 1392, 1566, 1611
BseNI ACTGG 5 cut(s) 231, 336, 483, 910, 1379
BsePI GCGCGC 1 cut(s) 204
BseRI GAGGAG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 8 cut(s) 250, 514, 988, 1504, 1532, 1586, 1621, 1662
BseYI CCCAGC 3 cut(s) 819, 1671, 1787
BsgI GTGCAG 3 cut(s) 552, 780, 1088
Bsh1236I CGCG 3 cut(s) 137, 206, 224
Bsh1285I CGRYCG 1 cut(s) 122
BshFI GGCC 6 cut(s) 92, 134, 230, 263, 1322, 1822
BshNI GGYRCC 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 1654
BsiEI CGRYCG 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 478
BsiSI CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
BslFI GGGAC 6 cut(s) 318, 639, 654, 802, 1268, 1670
BslI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1678, 1713, 1793
BsmAI GTCTC 5 cut(s) 162, 269, 348, 380, 1216
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 6 cut(s) 318, 639, 654, 802, 1268, 1670
BsnI GGCC 6 cut(s) 92, 134, 230, 263, 1322, 1822
Bsp1286I GDGCHC 2 cut(s) 478, 544
Bsp143I GATC 3 cut(s) 415, 432, 1096
Bsp19I CCATGG 1 cut(s) 1496
BspACI CCGC 6 cut(s) 105, 135, 222, 236, 242, 268
BspANI GGCC 6 cut(s) 92, 134, 230, 263, 1322, 1822
BspCNI CTCAG 4 cut(s) 1053, 1393, 1567, 1612
BspDI ATCGAT 1 cut(s) 1654
BspFNI CGCG 3 cut(s) 137, 206, 224
BspHI TCATGA 2 cut(s) 412, 1153
BspLI GGNNCC 6 cut(s) 333, 543, 868, 1168, 1383, 1821
BspMAI CTGCAG 3 cut(s) 964, 1522, 1680
BspMI ACCTGC 2 cut(s) 802, 976
BspOI GCTAGC 2 cut(s) 8, 1307
BspPI GGATC 1 cut(s) 427
BspT107I GGYRCC 1 cut(s) 866
BsrDI GCAATG 5 cut(s) 698, 737, 842, 927, 1644
BsrI ACTGG 5 cut(s) 231, 336, 483, 910, 1379
BssECI CCNNGG 6 cut(s) 93, 209, 327, 657, 707, 1496
BssHII GCGCGC 1 cut(s) 204
BssMI GATC 3 cut(s) 415, 432, 1096
BssT1I CCWWGG 1 cut(s) 1496
Bst2UI CCWGG 3 cut(s) 94, 753, 1131
Bst4CI ACNGT 6 cut(s) 179, 391, 653, 997, 1015, 1163
BstBAI YACGTR 2 cut(s) 383, 1342
BstC8I GCNNGC 7 cut(s) 6, 206, 228, 760, 1046, 1305, 1799
BstDEI CTNAG 6 cut(s) 807, 1040, 1401, 1575, 1592, 1620
BstDSI CCRYGG 2 cut(s) 657, 1496
BstF5I GGATG 8 cut(s) 264, 692, 703, 733, 1054, 1126, 1383, 1722
BstFNI CGCG 3 cut(s) 137, 206, 224
BstHHI GCGC 5 cut(s) 139, 206, 208, 226, 494
BstKTI GATC 3 cut(s) 418, 435, 1099
BstMAI GTCTC 5 cut(s) 162, 269, 348, 380, 1216
BstMBI GATC 3 cut(s) 415, 432, 1096
BstMCI CGRYCG 1 cut(s) 122
BstMWI GCNNNNNNNGC 5 cut(s) 25, 250, 873, 917, 982
BstNI CCWGG 3 cut(s) 94, 753, 1131
BstNSI RCATGY 1 cut(s) 1801
BstSCI CCNGG 6 cut(s) 92, 208, 327, 707, 751, 1129
BstSFI CTRYAG 4 cut(s) 960, 980, 1518, 1676
BstSLI GKGCMC 1 cut(s) 478
BstUI CGCG 3 cut(s) 137, 206, 224
BstV1I GCAGC 8 cut(s) 250, 514, 988, 1504, 1532, 1586, 1621, 1662
BstV2I GAAGAC 1 cut(s) 1768
BstX2I RGATCY 1 cut(s) 432
BstYI RGATCY 1 cut(s) 432
Bsu15I ATCGAT 1 cut(s) 1654
Bsu36I CCTNAGG 2 cut(s) 1575, 1592
BsuI GTATCC 1 cut(s) 1537
BsuRI GGCC 6 cut(s) 92, 134, 230, 263, 1322, 1822
BsuTUI ATCGAT 1 cut(s) 1654
BtgI CCRYGG 2 cut(s) 657, 1496
BtsCI GGATG 8 cut(s) 264, 692, 703, 733, 1054, 1126, 1383, 1722
BtsI GCAGTG 1 cut(s) 883
BtsIMutI CAGTG 6 cut(s) 343, 396, 883, 993, 1159, 1775
BveI ACCTGC 2 cut(s) 802, 976
Cac8I GCNNGC 7 cut(s) 6, 206, 228, 760, 1046, 1305, 1799
CaiI CAGNNNCTG 1 cut(s) 1181
CciI TCATGA 2 cut(s) 412, 1153
CfoI GCGC 5 cut(s) 139, 206, 208, 226, 494
Cfr13I GGNCC 1 cut(s) 1820
ClaI ATCGAT 1 cut(s) 1654
Csp6I GTAC 4 cut(s) 380, 779, 1072, 1464
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviQI GTAC 4 cut(s) 380, 779, 1072, 1464
DdeI CTNAG 6 cut(s) 807, 1040, 1401, 1575, 1592, 1620
DpnI GATC 3 cut(s) 417, 434, 1098
DpnII GATC 3 cut(s) 415, 432, 1096
DraI TTTAAA 1 cut(s) 1531
DraIII CACNNNGTG 1 cut(s) 776
DrdI GACNNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 3 cut(s) 228, 261, 1320
EciI GGCGGA 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 1496
Eco24I GRGCYC 1 cut(s) 544
Eco57I CTGAAG 2 cut(s) 433, 747
Eco81I CCTNAGG 2 cut(s) 1575, 1592
EcoO109I RGGNCCY 1 cut(s) 1820
EcoRII CCWGG 3 cut(s) 92, 751, 1129
EcoT14I CCWWGG 1 cut(s) 1496
EcoT22I ATGCAT 1 cut(s) 1410
EcoT38I GRGCYC 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 1496
Esp3I CGTCTC 1 cut(s) 269
FalI AAGNNNNNCTT 2 cut(s) 751, 783
FaqI GGGAC 6 cut(s) 318, 639, 654, 802, 1268, 1670
FauI CCCGC 1 cut(s) 98
FokI GGATG 8 cut(s) 251, 679, 690, 720, 1041, 1113, 1390, 1729
FriOI GRGCYC 1 cut(s) 544
FspBI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1304
FspI TGCGCA 1 cut(s) 493
GlaI GCGC 5 cut(s) 138, 205, 207, 225, 493
GsaI CCCAGC 3 cut(s) 823, 1675, 1791
GsuI CTGGAG 4 cut(s) 184, 774, 927, 1368
HaeIII GGCC 6 cut(s) 92, 134, 230, 263, 1322, 1822
HapII CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
HhaI GCGC 5 cut(s) 139, 206, 208, 226, 494
Hin6I GCGC 5 cut(s) 137, 204, 206, 224, 492
HinP1I GCGC 5 cut(s) 137, 204, 206, 224, 492
HincII GTYRAC 1 cut(s) 1629
HindII GTYRAC 1 cut(s) 1629
HindIII AAGCTT 1 cut(s) 851
HpaII CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
HphI GGTGA 3 cut(s) 762, 777, 1563
Hpy166II GTNNAC 6 cut(s) 428, 476, 779, 1482, 1629, 1775
Hpy188I TCNGA 6 cut(s) 217, 619, 727, 1402, 1426, 1829
Hpy188III TCNNGA 9 cut(s) 413, 549, 944, 1025, 1115, 1154, 1335, 1385, 1559
Hpy8I GTNNAC 6 cut(s) 428, 476, 779, 1482, 1629, 1775
Hpy99I CGWCG 4 cut(s) 70, 73, 76, 79
HpyAV CCTTC 7 cut(s) 82, 124, 683, 777, 919, 1505, 1534
HpyCH4III ACNGT 6 cut(s) 179, 391, 653, 997, 1015, 1163
HpyCH4IV ACGT 2 cut(s) 382, 1341
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 250, 873, 917, 982
HpyF3I CTNAG 6 cut(s) 807, 1040, 1401, 1575, 1592, 1620
HpySE526I ACGT 2 cut(s) 382, 1341
HspAI GCGC 5 cut(s) 137, 204, 206, 224, 492
Kzo9I GATC 3 cut(s) 415, 432, 1096
LmnI GCTCC 7 cut(s) 203, 337, 547, 755, 908, 1166, 1387
Lsp1109I GCAGC 8 cut(s) 250, 514, 988, 1504, 1532, 1586, 1621, 1662
LweI GCATC 5 cut(s) 698, 712, 1063, 1383, 1395
MaeI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1304
MaeII ACGT 2 cut(s) 382, 1341
MaeIII GTNAC 4 cut(s) 718, 991, 1003, 1569
MalI GATC 3 cut(s) 417, 434, 1098
MboI GATC 3 cut(s) 415, 432, 1096
MboII GAAGA 3 cut(s) 418, 1106, 1773
MfeI CAATTG 1 cut(s) 1746
MflI RGATCY 1 cut(s) 432
MhlI GDGCHC 2 cut(s) 478, 544
MlsI TGGCCA 2 cut(s) 230, 1322
MluCI AATT 6 cut(s) 157, 1086, 1270, 1720, 1746, 1802
MluNI TGGCCA 2 cut(s) 230, 1322
MlyI GAGTC 3 cut(s) 589, 824, 1735
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 2 cut(s) 230, 1322
Mph1103I ATGCAT 1 cut(s) 1410
MroXI GAANNNNTTC 3 cut(s) 317, 939, 1122
MscI TGGCCA 2 cut(s) 230, 1322
MseI TTAA 5 cut(s) 828, 1020, 1140, 1530, 1805
MslI CAYNNNNRTG 2 cut(s) 14, 1796
Msp20I TGGCCA 2 cut(s) 230, 1322
MspA1I CMGCKG 1 cut(s) 1675
MspI CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
MspR9I CCNGG 6 cut(s) 94, 210, 329, 709, 753, 1131
MunI CAATTG 1 cut(s) 1746
MvaI CCWGG 3 cut(s) 94, 753, 1131
MvnI CGCG 3 cut(s) 137, 206, 224
MwoI GCNNNNNNNGC 5 cut(s) 25, 250, 873, 917, 982
NciI CCSGG 3 cut(s) 210, 329, 709
NcoI CCATGG 1 cut(s) 1496
NdeII GATC 3 cut(s) 415, 432, 1096
NheI GCTAGC 2 cut(s) 4, 1303
NlaIV GGNNCC 6 cut(s) 333, 543, 868, 1168, 1383, 1821
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 2 cut(s) 991, 1569
NsbI TGCGCA 1 cut(s) 493
NsiI ATGCAT 1 cut(s) 1410
NspI RCATGY 1 cut(s) 1801
PaeI GCATGC 1 cut(s) 1801
PagI TCATGA 2 cut(s) 412, 1153
PauI GCGCGC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 3 cut(s) 65, 71, 77
PdmI GAANNNNTTC 3 cut(s) 317, 939, 1122
PfeI GAWTC 7 cut(s) 218, 257, 409, 504, 1283, 1348, 1783
PflFI GACNNNGTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 751
PleI GAGTC 3 cut(s) 589, 823, 1735
PpsI GAGTC 3 cut(s) 589, 823, 1735
Ppu21I YACGTR 2 cut(s) 383, 1342
PsiI TTATAA 2 cut(s) 188, 1661
Psp6I CCWGG 3 cut(s) 92, 751, 1129
PspFI CCCAGC 3 cut(s) 819, 1671, 1787
PspGI CCWGG 3 cut(s) 92, 751, 1129
PspN4I GGNNCC 6 cut(s) 333, 543, 868, 1168, 1383, 1821
PspPI GGNCC 1 cut(s) 1820
PsrI GAACNNNNNNTAC 2 cut(s) 622, 654
PstI CTGCAG 3 cut(s) 964, 1522, 1680
PstNI CAGNNNCTG 1 cut(s) 1181
PsuI RGATCY 1 cut(s) 432
PsyI GACNNNGTC 1 cut(s) 391
PteI GCGCGC 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 1675
RsaI GTAC 4 cut(s) 381, 780, 1073, 1465
RsaNI GTAC 4 cut(s) 380, 779, 1072, 1464
RseI CAYNNNNRTG 2 cut(s) 14, 1796
SaqAI TTAA 5 cut(s) 828, 1020, 1140, 1530, 1805
Sau3AI GATC 3 cut(s) 415, 432, 1096
Sau96I GGNCC 1 cut(s) 1820
ScaI AGTACT 1 cut(s) 1073
SchI GAGTC 3 cut(s) 589, 824, 1735
ScrFI CCNGG 6 cut(s) 94, 210, 329, 709, 753, 1131
SduI GDGCHC 2 cut(s) 478, 544
SfaNI GCATC 5 cut(s) 698, 712, 1063, 1383, 1395
SfcI CTRYAG 4 cut(s) 960, 980, 1518, 1676
SmiMI CAYNNNNRTG 2 cut(s) 14, 1796
SmlI CTYRAG 1 cut(s) 1049
SmoI CTYRAG 1 cut(s) 1049
SphI GCATGC 1 cut(s) 1801
Sse9I AATT 6 cut(s) 157, 1086, 1270, 1720, 1746, 1802
SsiI CCGC 6 cut(s) 105, 135, 222, 236, 242, 268
SspI AATATT 1 cut(s) 1063
SspMI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1304
StyD4I CCNGG 6 cut(s) 92, 208, 327, 707, 751, 1129
StyI CCWWGG 1 cut(s) 1496
TaaI ACNGT 6 cut(s) 179, 391, 653, 997, 1015, 1163
TaiI ACGT 2 cut(s) 385, 1344
TaqI TCGA 6 cut(s) 122, 143, 418, 456, 676, 1654
TasI AATT 6 cut(s) 157, 1086, 1270, 1720, 1746, 1802
TatI WGTACW 2 cut(s) 1071, 1463
TauI GCSGC 2 cut(s) 137, 244
TfiI GAWTC 7 cut(s) 218, 257, 409, 504, 1283, 1348, 1783
Tru1I TTAA 5 cut(s) 828, 1020, 1140, 1530, 1805
Tru9I TTAA 5 cut(s) 828, 1020, 1140, 1530, 1805
TscAI CASTG 6 cut(s) 343, 396, 883, 1000, 1166, 1782
TseFI GTSAC 2 cut(s) 991, 1569
TseI GCWGC 8 cut(s) 238, 527, 976, 1517, 1520, 1599, 1634, 1675
Tsp45I GTSAC 2 cut(s) 991, 1569
TspDTI ATGAA 9 cut(s) 648, 938, 1115, 1142, 1170, 1275, 1524, 1553, 1733
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 6 cut(s) 343, 396, 883, 1000, 1166, 1782
Tth111I GACNNNGTC 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 21
VneI GTGCAC 1 cut(s) 474
XapI RAATTY 1 cut(s) 1720
XceI RCATGY 1 cut(s) 1801
XcmI CCANNNNNNNNNTGG 1 cut(s) 1211
XmnI GAANNNNTTC 3 cut(s) 317, 939, 1122
XspI CTAG 7 cut(s) 5, 30, 399, 501, 663, 774, 1304
ZrmI AGTACT 1 cut(s) 1073
Zsp2I ATGCAT 1 cut(s) 1410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.