FvH4_2g40740

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0001271
Physical Location & Seq
Forward (+)
28973380 .. 28979498
6119 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40740.t2

Sequence Viewer

Length: 2145 bp
ATGACTCATTTTGATATATTAGGAGGGGGCCTTCTGACAATTTCAACCGACGGCTTCGACCAGAACCACACCACCGCCGCCACCACTTGCCACCGCCTACCACTTCCCAATCACCTCATGCCTTCTTCGTCTTCTTCTCCATCCCCTTCTCAACCAAAAGGACCTGTTTCCTCCTCCTCCTCTGTCCCAGCTCAGAGACCGGGGATCATCAGAAGCGGCAGCGATGTTAACAGCCCACGGCGGCTCACGCGCCAGGGGAGGCTGAGCAAGGATGTGATTTCCTACTCCGTCCCGGAGAAGTCTCGCTCATCGCCCACGTCTCCTGAATATTCGACCCGTAAATCACGCTCTCCCAGTGCCGCCGAGTCGGCCTCGACCCCTAGTTCTCGCTCCTCGGTAGGTGACTCGGCCCGGAAGTCATGCTCTTCTTCTTCTTTCGTCGCTGGGCCTCAAGGAAGCCCACATCCCCTGCCGCTCCCGGAATTTCCGGTGACCAACCGGAGACCCGACTGCGGCTCTAAAGATAACAACTTCTTCAGCAGGAAGAGGACTGATCAAGCGGGAACAACCTCATCGTCGGCGAAAGCATCCAAGAATTACCGCCAAGATGTAAAGGGTGGGGATGGTGTTTTCTACTTCAACTGGTTGAATGTTCCATCTAGGACTAAAACGGAGTGTGCTCCACAGTTGAGCCAACGGGGTCATCATCCTGTTAGCCCACAAAGAGCAAAGACGATGGATTTTTTGCCTACACATTCTTCATATGTTGCGGCGAACAATTACAACAGGGGAATGGGAATGAGAGTGTCAACGGAGGCTAACTTTGAACGTGAAAGTTACAACTTGAGCAGGAGTGCTCCAACTAGTGTTTTCTCAAGTCCTGCAGTTAGCCCCCGCAGATCAAACGTTGCGGATCCTTTTCCTTTTTTCACACCTCATCAGGAGAAATATCAGGATGGTGGTGCTACTTTCCCTTCATTGTTGTTGTCACCCAAGAAAACTACACCTACGCTTAGTCCCAATCATTCTCCACTTCATAGCCCTACGCCTCGAAGTCCACGCTTCAGCCCCAATGCTTCAAACTACGACACTAATAGTCTTGCCACTGCTCACCCGTTGCCTTTGCCTCCTGGAGCCGCGGTGGCACCACCACCGCAATCTATCGTTATGCATCAAAATGTTGCACCGAATGTAGCTGTGACGTCAATGAAAGGGCAGTGGCAGAAGGGGAAACTTATTGGACGGGGTACCTTTGGTAGTGTTTATCTTGCAACCAACAGAGAAACTGGGGCCTTGTGTGCCATGAAGGAAGTTGATCTCATTCCTGATGATCCTAAATCTGCAGAAAGTATCAAGCAATTGGAACAGGAAATTAAAGTCCTCCGCACACTAAAGCATCCAAATATTGTGCAGTATTATGGTAGTGAAGTGATTGATGATCATTTCTACATATATTTAGAATATGTTCACCCTGGATCAATAAATAGATATGTCCAAGACCATTTTGGAGCCATGACGGAATCTGTAGTTCGCAATTTTACCCGCCATATCCTCTATGGGTTGGCTTATTTGCATGACACGAAGACAATTCACAGGGATATTAAAGGTGCAAATTTGCTTGTTGATGCATCAGGCGTCGTCAAACTTGCCGATTTTGGGATGGCAAAACATCTTAGTGCACATTCATATAATCTTTCTTTGAAGGGCAGCCCGTACTGGATGGCTCCTGAGGTCATAAAGGCTGTGATTCAGAATGATGGTGATCCTAATCTTGCTTTGGCTGTTGATATATGGAGCTTGGGTTGCACCATCATTGAGATGTTCAATGGAAAACCGCCTTGGAGTGAATTTGAAGGGCCACAAGCTATGTTCAAGGTTCTGCACAGAACCCCGGATATACCAGAAAGATTATCTGCAGAAGGAAAAGAGTTCCTAAGTTTGTGCTTCAAAAGGAACCCTGCAGAGAGACCATCAGCCAAGAAGCTACTCGATCATCCTTTTGTACGAATTTCAAATGACCAAAATGTCTCATCTTATAGTCGAGCTTTTTCTCTGGTAAACCTAATGGACAAACTGAATAGCCCGACGGACCACATAAAGCAAAAACTTGGTCGAGTGTTGGTCGGTTCACGCACTTCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

715

Amino Acids

78.16

Weight (kDa)

9.58

Isoelectric Point (pI)

56.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 439 - 700 1.4e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 442 - 695 8.8e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2024
AatII GACGTC 1 cut(s) 1205
Acc65I GGTACC 1 cut(s) 1247
AccB1I GGYRCC 2 cut(s) 1144, 1247
AccBSI CCGCTC 1 cut(s) 475
AccII CGCG 2 cut(s) 250, 1139
AclI AACGTT 1 cut(s) 906
AclWI GGATC 6 cut(s) 212, 908, 921, 1325, 1483, 1757
AcsI RAATTY 4 cut(s) 482, 1612, 1847, 2007
AcuI CTGAAG 2 cut(s) 520, 1048
AcyI GRCGYC 2 cut(s) 1202, 1635
AfaI GTAC 3 cut(s) 1249, 1715, 2004
AfiI CCNNNNNNNGG 2 cut(s) 512, 1656
AhlI ACTAGT 1 cut(s) 863
AjiI CACGTC 1 cut(s) 318
AjnI CCWGG 3 cut(s) 252, 1129, 1471
AjuI GAANNNNNNNTTGG 2 cut(s) 1822, 1854
AluBI AGCT 6 cut(s) 191, 1196, 1797, 1865, 1984, 2045
AluI AGCT 6 cut(s) 191, 1196, 1797, 1865, 1984, 2045
Alw21I GWGCWC 3 cut(s) 682, 859, 1681
Alw26I GTCTC 6 cut(s) 190, 306, 324, 496, 1960, 2032
Alw44I GTGCAC 1 cut(s) 1677
AlwI GGATC 6 cut(s) 212, 908, 921, 1325, 1483, 1757
AoxI GGCC 6 cut(s) 28, 369, 408, 446, 1290, 1856
ApaLI GTGCAC 1 cut(s) 1677
ApeKI GCWGC 2 cut(s) 219, 1707
ApoI RAATTY 4 cut(s) 482, 1612, 1847, 2007
ArsI GACNNNNNNTTYG 2 cut(s) 2095, 2127
Asp700I GAANNNNTTC 1 cut(s) 1464
Asp718I GGTACC 1 cut(s) 1247
AspLEI GCGC 1 cut(s) 252
AspS9I GGNCC 7 cut(s) 28, 161, 409, 446, 1290, 1856, 2089
AsuC2I CCSGG 5 cut(s) 201, 293, 412, 479, 1892
AsuHPI GGTGA 7 cut(s) 104, 413, 502, 981, 1103, 1460, 1772
AvaII GGWCC 2 cut(s) 161, 2089
AxyI CCTNAGG 1 cut(s) 1728
BaeGI GKGCMC 1 cut(s) 1681
BamHI GGATCC 1 cut(s) 913
BanI GGYRCC 2 cut(s) 1144, 1247
BbsI GAAGAC 2 cut(s) 123, 1589
Bbv12I GWGCWC 3 cut(s) 682, 859, 1681
BbvI GCAGC 2 cut(s) 231, 1719
BceAI ACGGC 2 cut(s) 67, 254
BciT130I CCWGG 3 cut(s) 254, 1131, 1473
BclI TGATCA 2 cut(s) 553, 1438
BcnI CCSGG 5 cut(s) 201, 293, 412, 479, 1892
BcoDI GTCTC 6 cut(s) 190, 306, 324, 496, 1960, 2032
BcuI ACTAGT 1 cut(s) 863
BfaI CTAG 3 cut(s) 381, 660, 864
BfmI CTRYAG 5 cut(s) 882, 1341, 1524, 1914, 1959
BglI GCCNNNNNGGC 2 cut(s) 368, 1142
BlpI GCTNAGC 1 cut(s) 263
Bme1390I CCNGG 8 cut(s) 201, 254, 293, 412, 479, 1131, 1473, 1892
Bme18I GGWCC 2 cut(s) 161, 2089
BmgBI CACGTC 1 cut(s) 318
BmgT120I GGNCC 7 cut(s) 28, 161, 409, 446, 1290, 1856, 2089
BmiI GGNNCC 9 cut(s) 29, 915, 1135, 1146, 1249, 1291, 1510, 1725, 1955
BmrFI CCNGG 8 cut(s) 201, 254, 293, 412, 479, 1131, 1473, 1892
BmrI ACTGGG 2 cut(s) 348, 1296
BmsI GCATC 5 cut(s) 596, 1180, 1405, 1615, 1637
BmuI ACTGGG 2 cut(s) 348, 1296
BpiI GAAGAC 2 cut(s) 123, 1589
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 1 cut(s) 1152
Bpu1102I GCTNAGC 1 cut(s) 263
BpuEI CTTGAG 3 cut(s) 435, 859, 865
BpuMI CCSGG 5 cut(s) 201, 293, 412, 479, 1892
BsaBI GATNNNNATC 2 cut(s) 1761, 1767
BsaHI GRCGYC 2 cut(s) 1202, 1635
BsaI GGTCTC 3 cut(s) 190, 496, 1960
BsaJI CCNNGG 8 cut(s) 200, 236, 253, 393, 1137, 1471, 1838, 1890
BsaWI WCCGGW 2 cut(s) 487, 498
BsaXI ACNNNNNCTCC 4 cut(s) 1125, 1155, 1786, 1816
Bsc4I CCNNNNNNNGG 2 cut(s) 512, 1656
Bse1I ACTGG 4 cut(s) 354, 647, 1291, 1721
Bse21I CCTNAGG 1 cut(s) 1728
Bse8I GATNNNNATC 2 cut(s) 1761, 1767
BseBI CCWGG 3 cut(s) 254, 1131, 1473
BseDI CCNNGG 8 cut(s) 200, 236, 253, 393, 1137, 1471, 1838, 1890
BseJI GATNNNNATC 2 cut(s) 1761, 1767
BseLI CCNNNNNNNGG 2 cut(s) 512, 1656
BseMII CTCAG 3 cut(s) 206, 254, 1719
BseNI ACTGG 4 cut(s) 354, 647, 1291, 1721
BseRI GAGGAG 4 cut(s) 163, 166, 169, 382
BseSI GKGCMC 1 cut(s) 1681
BseXI GCAGC 2 cut(s) 231, 1719
BseYI CCCAGC 2 cut(s) 187, 443
BsgI GTGCAG 2 cut(s) 1430, 1865
Bsh1236I CGCG 2 cut(s) 250, 1139
BshFI GGCC 6 cut(s) 30, 371, 410, 448, 1292, 1858
BshNI GGYRCC 2 cut(s) 1144, 1247
BsiHKAI GWGCWC 3 cut(s) 682, 859, 1681
BsiSI CCGG 7 cut(s) 200, 293, 412, 479, 488, 499, 1892
BslFI GGGAC 3 cut(s) 170, 275, 1002
BslI CCNNNNNNNGG 2 cut(s) 512, 1656
BsmAI GTCTC 6 cut(s) 190, 306, 324, 496, 1960, 2032
BsmBI CGTCTC 1 cut(s) 324
BsmFI GGGAC 3 cut(s) 170, 275, 1002
BsnI GGCC 6 cut(s) 30, 371, 410, 448, 1292, 1858
Bso31I GGTCTC 3 cut(s) 190, 496, 1960
Bsp1286I GDGCHC 3 cut(s) 682, 859, 1681
Bsp1720I GCTNAGC 1 cut(s) 263
BspANI GGCC 6 cut(s) 30, 371, 410, 448, 1292, 1858
BspCNI CTCAG 3 cut(s) 205, 255, 1720
BspFNI CGCG 2 cut(s) 250, 1139
BspLI GGNNCC 9 cut(s) 29, 915, 1135, 1146, 1249, 1291, 1510, 1725, 1955
BspMAI CTGCAG 4 cut(s) 886, 1345, 1918, 1963
BspPI GGATC 6 cut(s) 212, 908, 921, 1325, 1483, 1757
BspQI GCTCTTC 1 cut(s) 430
BspT107I GGYRCC 2 cut(s) 1144, 1247
BspTNI GGTCTC 3 cut(s) 190, 496, 1960
BsrBI CCGCTC 1 cut(s) 475
BsrI ACTGG 4 cut(s) 354, 647, 1291, 1721
BssECI CCNNGG 8 cut(s) 200, 236, 253, 393, 1137, 1471, 1838, 1890
BssNI GRCGYC 2 cut(s) 1202, 1635
BssT1I CCWWGG 1 cut(s) 1838
Bst2UI CCWGG 3 cut(s) 254, 1131, 1473
Bst4CI ACNGT 1 cut(s) 687
Bst6I CTCTTC 2 cut(s) 430, 539
BstACI GRCGYC 2 cut(s) 1202, 1635
BstDEI CTNAG 6 cut(s) 192, 263, 1013, 1673, 1728, 1934
BstDSI CCRYGG 2 cut(s) 236, 1137
BstEII GGTNACC 1 cut(s) 490
BstFNI CGCG 2 cut(s) 250, 1139
BstHHI GCGC 1 cut(s) 252
BstMAI GTCTC 6 cut(s) 190, 306, 324, 496, 1960, 2032
BstMWI GCNNNNNNNGC 5 cut(s) 247, 368, 1142, 1298, 1803
BstNI CCWGG 3 cut(s) 254, 1131, 1473
BstPI GGTNACC 1 cut(s) 490
BstSCI CCNGG 8 cut(s) 199, 252, 291, 410, 477, 1129, 1471, 1890
BstSFI CTRYAG 5 cut(s) 882, 1341, 1524, 1914, 1959
BstSLI GKGCMC 1 cut(s) 1681
BstUI CGCG 2 cut(s) 250, 1139
BstV1I GCAGC 2 cut(s) 231, 1719
BstV2I GAAGAC 2 cut(s) 123, 1589
BstX2I RGATCY 1 cut(s) 913
BstYI RGATCY 1 cut(s) 913
Bsu36I CCTNAGG 1 cut(s) 1728
BsuRI GGCC 6 cut(s) 30, 371, 410, 448, 1292, 1858
BtgI CCRYGG 2 cut(s) 236, 1137
BtgZI GCGATG 2 cut(s) 237, 294
BtrI CACGTC 1 cut(s) 318
BtsI GCAGTG 2 cut(s) 1104, 1223
BtsIMutI CAGTG 3 cut(s) 361, 1104, 1223
CfoI GCGC 1 cut(s) 252
Cfr13I GGNCC 7 cut(s) 28, 161, 409, 446, 1290, 1856, 2089
Cfr42I CCGCGG 1 cut(s) 1140
CseI GACGC 1 cut(s) 1624
Csp6I GTAC 3 cut(s) 1248, 1714, 2003
CviAII CATG 5 cut(s) 118, 420, 1303, 1513, 1574
CviQI GTAC 3 cut(s) 1248, 1714, 2003
DdeI CTNAG 6 cut(s) 192, 263, 1013, 1673, 1728, 1934
DrdI GACNNNNNNGTC 1 cut(s) 2024
DseDI GACNNNNNNGTC 1 cut(s) 2024
Eam1104I CTCTTC 2 cut(s) 430, 539
EarI CTCTTC 2 cut(s) 430, 539
Eco130I CCWWGG 1 cut(s) 1838
Eco31I GGTCTC 3 cut(s) 190, 496, 1960
Eco47I GGWCC 2 cut(s) 161, 2089
Eco57I CTGAAG 2 cut(s) 520, 1048
Eco81I CCTNAGG 1 cut(s) 1728
Eco91I GGTNACC 1 cut(s) 490
EcoO109I RGGNCCY 3 cut(s) 28, 161, 1290
EcoO65I GGTNACC 1 cut(s) 490
EcoRII CCWGG 3 cut(s) 252, 1129, 1471
EcoT14I CCWWGG 1 cut(s) 1838
EcoT22I ATGCAT 2 cut(s) 1173, 1630
ErhI CCWWGG 1 cut(s) 1838
Esp3I CGTCTC 1 cut(s) 324
FaeI CATG 5 cut(s) 121, 423, 1306, 1516, 1577
FaqI GGGAC 3 cut(s) 170, 275, 1002
FatI CATG 5 cut(s) 117, 419, 1302, 1512, 1573
FauI CCCGC 3 cut(s) 553, 902, 1550
FauNDI CATATG 1 cut(s) 763
FbaI TGATCA 2 cut(s) 553, 1438
FspBI CTAG 3 cut(s) 381, 660, 864
GlaI GCGC 1 cut(s) 251
GsaI CCCAGC 2 cut(s) 191, 447
GsuI CTGGAG 1 cut(s) 1152
HaeIII GGCC 6 cut(s) 30, 371, 410, 448, 1292, 1858
HapII CCGG 7 cut(s) 200, 293, 412, 479, 488, 499, 1892
HgaI GACGC 1 cut(s) 1624
HhaI GCGC 1 cut(s) 252
Hin1I GRCGYC 2 cut(s) 1202, 1635
Hin1II CATG 5 cut(s) 121, 423, 1306, 1516, 1577
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HincII GTYRAC 2 cut(s) 229, 810
HindII GTYRAC 2 cut(s) 229, 810
HinfI GANTC 5 cut(s) 4, 365, 404, 1520, 1747
HpaI GTTAAC 1 cut(s) 229
HpaII CCGG 7 cut(s) 200, 293, 412, 479, 488, 499, 1892
HphI GGTGA 7 cut(s) 104, 413, 502, 981, 1103, 1460, 1772
Hpy166II GTNNAC 7 cut(s) 229, 810, 1058, 1468, 1679, 2059, 2129
Hpy188I TCNGA 4 cut(s) 36, 195, 212, 1752
Hpy188III TCNNGA 5 cut(s) 323, 941, 953, 1325, 1727
Hpy8I GTNNAC 7 cut(s) 229, 810, 1058, 1468, 1679, 2059, 2129
Hpy99I CGWCG 5 cut(s) 53, 443, 580, 1640, 2089
HpyAV CCTTC 9 cut(s) 41, 132, 156, 984, 1219, 1300, 1696, 1847, 1913
HpyCH4III ACNGT 1 cut(s) 687
HpyCH4IV ACGT 4 cut(s) 317, 829, 906, 1202
HpyF10VI GCNNNNNNNGC 5 cut(s) 247, 368, 1142, 1298, 1803
HpyF3I CTNAG 6 cut(s) 192, 263, 1013, 1673, 1728, 1934
HpySE526I ACGT 4 cut(s) 317, 829, 906, 1202
Hsp92I GRCGYC 2 cut(s) 1202, 1635
Hsp92II CATG 5 cut(s) 121, 423, 1306, 1516, 1577
HspAI GCGC 1 cut(s) 250
KpnI GGTACC 1 cut(s) 1251
Ksp22I TGATCA 2 cut(s) 553, 1438
KspAI GTTAAC 1 cut(s) 229
KspI CCGCGG 1 cut(s) 1140
LguI GCTCTTC 1 cut(s) 430
LmnI GCTCC 8 cut(s) 395, 480, 685, 862, 1133, 1508, 1729, 1794
Lsp1109I GCAGC 2 cut(s) 231, 1719
LweI GCATC 5 cut(s) 596, 1180, 1405, 1615, 1637
MaeI CTAG 3 cut(s) 381, 660, 864
MaeII ACGT 4 cut(s) 317, 829, 906, 1202
MaeIII GTNAC 5 cut(s) 401, 490, 836, 987, 1198
MbiI CCGCTC 1 cut(s) 475
MfeI CAATTG 1 cut(s) 1358
MflI RGATCY 1 cut(s) 913
MhlI GDGCHC 3 cut(s) 682, 859, 1681
MlyI GAGTC 2 cut(s) 374, 398
MmeI TCCRAC 1 cut(s) 884
Mph1103I ATGCAT 2 cut(s) 1173, 1630
MroXI GAANNNNTTC 1 cut(s) 1464
MseI TTAA 3 cut(s) 228, 1374, 1602
MslI CAYNNNNRTG 3 cut(s) 1176, 1674, 1817
MspA1I CMGCKG 1 cut(s) 1139
MspI CCGG 7 cut(s) 200, 293, 412, 479, 488, 499, 1892
MspR9I CCNGG 8 cut(s) 201, 254, 293, 412, 479, 1131, 1473, 1892
MunI CAATTG 1 cut(s) 1358
MvaI CCWGG 3 cut(s) 254, 1131, 1473
MvnI CGCG 2 cut(s) 250, 1139
MwoI GCNNNNNNNGC 5 cut(s) 247, 368, 1142, 1298, 1803
NciI CCSGG 5 cut(s) 201, 293, 412, 479, 1892
NdeI CATATG 1 cut(s) 763
NlaIII CATG 5 cut(s) 121, 423, 1306, 1516, 1577
NlaIV GGNNCC 9 cut(s) 29, 915, 1135, 1146, 1249, 1291, 1510, 1725, 1955
NmeAIII GCCGAG 2 cut(s) 386, 388
NmuCI GTSAC 4 cut(s) 401, 490, 987, 1198
NsiI ATGCAT 2 cut(s) 1173, 1630
PciSI GCTCTTC 1 cut(s) 430
PdmI GAANNNNTTC 1 cut(s) 1464
PfeI GAWTC 2 cut(s) 1520, 1747
PfoI TCCNGGA 3 cut(s) 291, 477, 1129
PleI GAGTC 2 cut(s) 373, 398
PpsI GAGTC 2 cut(s) 373, 398
PpuMI RGGWCCY 1 cut(s) 161
Psp1406I AACGTT 1 cut(s) 906
Psp5II RGGWCCY 1 cut(s) 161
Psp6I CCWGG 3 cut(s) 252, 1129, 1471
PspEI GGTNACC 1 cut(s) 490
PspFI CCCAGC 2 cut(s) 187, 443
PspGI CCWGG 3 cut(s) 252, 1129, 1471
PspN4I GGNNCC 9 cut(s) 29, 915, 1135, 1146, 1249, 1291, 1510, 1725, 1955
PspPI GGNCC 7 cut(s) 28, 161, 409, 446, 1290, 1856, 2089
PspPPI RGGWCCY 1 cut(s) 161
PstI CTGCAG 4 cut(s) 886, 1345, 1918, 1963
PsuI RGATCY 1 cut(s) 913
RsaI GTAC 3 cut(s) 1249, 1715, 2004
RsaNI GTAC 3 cut(s) 1248, 1714, 2003
RseI CAYNNNNRTG 3 cut(s) 1176, 1674, 1817
SacII CCGCGG 1 cut(s) 1140
SapI GCTCTTC 1 cut(s) 430
SaqAI TTAA 3 cut(s) 228, 1374, 1602
Sau96I GGNCC 7 cut(s) 28, 161, 409, 446, 1290, 1856, 2089
SchI GAGTC 2 cut(s) 374, 398
ScrFI CCNGG 8 cut(s) 201, 254, 293, 412, 479, 1131, 1473, 1892
SduI GDGCHC 3 cut(s) 682, 859, 1681
SfaNI GCATC 5 cut(s) 596, 1180, 1405, 1615, 1637
SfcI CTRYAG 5 cut(s) 882, 1341, 1524, 1914, 1959
Sfr303I CCGCGG 1 cut(s) 1140
SgrBI CCGCGG 1 cut(s) 1140
SinI GGWCC 2 cut(s) 161, 2089
SmiMI CAYNNNNRTG 3 cut(s) 1176, 1674, 1817
SmlI CTYRAG 3 cut(s) 450, 844, 874
SmoI CTYRAG 3 cut(s) 450, 844, 874
SpeI ACTAGT 1 cut(s) 863
SspI AATATT 2 cut(s) 329, 1405
SspMI CTAG 3 cut(s) 381, 660, 864
StyD4I CCNGG 8 cut(s) 199, 252, 291, 410, 477, 1129, 1471, 1890
StyI CCWWGG 1 cut(s) 1838
TaaI ACNGT 1 cut(s) 687
TaiI ACGT 4 cut(s) 320, 832, 909, 1205
TaqI TCGA 7 cut(s) 57, 332, 374, 1051, 1989, 2041, 2113
TauI GCSGC 8 cut(s) 80, 219, 244, 362, 475, 516, 773, 1139
TfiI GAWTC 2 cut(s) 1520, 1747
Tru1I TTAA 3 cut(s) 228, 1374, 1602
Tru9I TTAA 3 cut(s) 228, 1374, 1602
TscAI CASTG 3 cut(s) 361, 1111, 1223
TseFI GTSAC 4 cut(s) 401, 490, 987, 1198
TseI GCWGC 2 cut(s) 219, 1707
Tsp45I GTSAC 4 cut(s) 401, 490, 987, 1198
TspDTI ATGAA 6 cut(s) 750, 966, 1025, 1223, 1319, 1674
TspGWI ACGGA 5 cut(s) 277, 686, 827, 1532, 2102
TspRI CASTG 3 cut(s) 361, 1111, 1223
VneI GTGCAC 1 cut(s) 1677
VpaK11BI GGWCC 2 cut(s) 161, 2089
XapI RAATTY 4 cut(s) 482, 1612, 1847, 2007
XcmI CCANNNNNNNNNTGG 2 cut(s) 1502, 1553
XmnI GAANNNNTTC 1 cut(s) 1464
XspI CTAG 3 cut(s) 381, 660, 864
ZraI GACGTC 1 cut(s) 1203
Zsp2I ATGCAT 2 cut(s) 1173, 1630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.