RLG00000017359

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001271
Physical Location & Seq
Forward (+)
15631343 .. 15635845
4503 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017359

Sequence Viewer

Length: 1788 bp
ATGCTAGCCACAACCATGCGTTGGTTGCCTAGCTTTGCTTCCTCATCATCATCATCTGCTTCGTCTTCGTCGTCGCCGAAAAAGAAGGCCAGGGAGGACGGCGGGTGGATTTTCCGGTCGAGGAGGAAGGCCGCGCAGTCGAGGAAACTGCTCAATTTGGGGAGAGACAATGACGGTGGAGCTATAACCAAATGCTCCAGCGCACCGGGGTCGGAATCCGCGCTGGCCAGTCCGCAGCCGCTTCCTTTGCCGGAATCGGCCATCAGCCGAGAGACGAGGTGTTCTCGCTGTAAGTCATGGGGACATGAGAACTGCTTCCCCTCCCCGGCTCCCAGTGCGTTTTCTTGGCGAGACACGCCAAAGAATAACGTTCAAGTACGTGAGACAGTGTCGTCTAGGAGGAAGAATCAAGATCGAAATCGTTTACAGATCCCTGAACATGACTTCAGGCTCGATGTTCCTGTTAGAAGTGCACCCACTAGTCCATGCGCAAGTCCTAGATTCAGCCCACACAGACAAAGTGCTGCTGATTTGTGGGCTCCGTCCAGAAATCAAGTCTGGTCTGCACCGGAGATGACTACTAATGATATGACTCAACCTCCTCCTGCCTTATCAGATGTGTTTAGTAATGAAAGTTCCCCTATAAACAGTCCCACGGCTAGAAGTCCCCTTCGACCCCCACAAGGATCATCCCCATTGCATCCCCGGTTATCAGTTACCTCTGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGCCTCCCTTCACCTAGTGTACCATCACCACAACCTGCACTCACACCTAAGCCAGAGTCCCAGCCGATTAAAGGGCAATGGCAAAAGGGAAAGCTTATTGGCCGTGGCACCTTTGGCAGTGTTTATGTTGCTACCAATAGAGAAACTGGAGCTTTATGTGCAATGAAGGAAGTTGAACTACTTCCTGATGACCCGAAATCAGCAGAGTGTATAAAGCAGCTACAGCAGGAAATCAAAGTCCTCAGCCAGCTCAAGCATCCTAATATTGTGCAGTACTATGGAAGTGAAATTGTTGAAGATCGGTTTTTTATATATCTGGAGTATGTTCATCCAGGTTCTATTAACAAATATGTTCATGAACACTGTGGAGCCATGACAGAAGCAGTTGTTCGCAGTTTTACTCGCCATATAGTCTCTGGGTTGGCTTACTTGCACAGCACAAAAACAATACACAGGGACATCAAAGGGGCTAATTTGCTGGTGGATTCATGTGGGGTTGTCAAGTTAGCTGACTTTGGTATGGCAAAACATCTTTCAGGACACGTAGGGAATCTTTCCCTAAAGGGAAGTCCATACTGGATGGCTCCGGAGCTGATGCAATCTGAGATGCATAAAGATAACAGCTCTGATTTGGCTCTTGCTGTTGATATATGGAGTTTGGGTTGTACTATTATTGAAATGTTCACAGGAAAACCTCCATGGAGTGAGTATGAAGGGGCTGCAGCTATGTTTAAAGTTATGAAGGATACTCCACCATTTCCAGAAACATTGTCGCCTGAGGGTAAGAATTTCCTTAGGTGCTGCTTCCAAAGAAATCCTGCTGAGAGGTCAACTGCTGCAGTGCTATTAGAACATCGATTTATTAAAAACTCCCAGCCACAGGAAGTCCCATCCACGACCTGTAATGCCTCTAATGGGATGAATTTCTTGGAAAGACCCCAGACTCCAATTGAACTTGAAAGAAGACTCGATAGTTCACTGATGATTCCTAGCATTGGCATGCAATTAACTACGGGAAAGGCGGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

596

Amino Acids

65.38

Weight (kDa)

9.12

Isoelectric Point (pI)

62.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 279 - 540 1.1e-66 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 282 - 535 4.1e-44 Protein tyrosine and serine/threonine kinase
Kinase-like PF14531 385 - 491 7.9e-06 Kinase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 391
Acc16I TGCGCA 1 cut(s) 490
Acc36I ACCTGC 1 cut(s) 799
AccB1I GGYRCC 1 cut(s) 863
AccB7I CCANNNNNTGG 1 cut(s) 21
AccII CGCG 2 cut(s) 134, 221
AccIII TCCGGA 1 cut(s) 1342
AciI CCGC 6 cut(s) 102, 132, 219, 233, 239, 1778
AclI AACGTT 1 cut(s) 369
AclWI GGATC 2 cut(s) 424, 694
AcoI YGGCCR 3 cut(s) 225, 258, 856
AcsI RAATTY 2 cut(s) 1543, 1678
AcuI CTGAAG 2 cut(s) 430, 744
AdeI CACNNNGTG 1 cut(s) 773
AfaI GTAC 4 cut(s) 378, 777, 1031, 1423
AfiI CCNNNNNNNGG 7 cut(s) 21, 325, 683, 827, 1636, 1671, 1751
AflIII ACRYGT 1 cut(s) 1297
AgsI TTSAA 6 cut(s) 374, 932, 1052, 1433, 1709, 1715
AhlI ACTAGT 1 cut(s) 479
AjnI CCWGG 3 cut(s) 89, 748, 1087
Alw21I GWGCWC 1 cut(s) 475
Alw26I GTCTC 5 cut(s) 159, 266, 345, 377, 1174
Alw44I GTGCAC 1 cut(s) 471
AlwI GGATC 2 cut(s) 424, 694
Aor13HI TCCGGA 1 cut(s) 1342
AoxI GGCC 5 cut(s) 87, 129, 225, 258, 856
ApaLI GTGCAC 1 cut(s) 471
ApeKI GCWGC 7 cut(s) 235, 524, 973, 1475, 1478, 1557, 1592
ApoI RAATTY 2 cut(s) 1543, 1678
Asp700I GAANNNNTTC 2 cut(s) 314, 936
AspLEI GCGC 4 cut(s) 136, 203, 223, 491
AsuC2I CCSGG 3 cut(s) 207, 326, 706
AsuHPI GGTGA 2 cut(s) 759, 774
AsuNHI GCTAGC 1 cut(s) 4
AxyI CCTNAGG 2 cut(s) 1533, 1550
BaeGI GKGCMC 1 cut(s) 475
BalI TGGCCA 1 cut(s) 227
BanI GGYRCC 1 cut(s) 863
BanII GRGCYC 1 cut(s) 541
BarI GAAGNNNNNNTAC 2 cut(s) 918, 950
BbsI GAAGAC 2 cut(s) 57, 1726
Bbv12I GWGCWC 1 cut(s) 475
BbvCI CCTCAGC 1 cut(s) 998
BbvI GCAGC 7 cut(s) 247, 511, 985, 1462, 1490, 1544, 1579
BccI CCATC 5 cut(s) 269, 738, 787, 1330, 1654
BceAI ACGGC 3 cut(s) 115, 672, 843
BcgI CGANNNNNNTGC 4 cut(s) 130, 164, 192, 226
BciT130I CCWGG 3 cut(s) 91, 750, 1089
BciVI GTATCC 1 cut(s) 1495
BcnI CCSGG 3 cut(s) 207, 326, 706
BcoDI GTCTC 5 cut(s) 159, 266, 345, 377, 1174
BcuI ACTAGT 1 cut(s) 479
BfaI CTAG 8 cut(s) 5, 30, 396, 480, 498, 660, 771, 1746
BfmI CTRYAG 3 cut(s) 977, 1476, 1593
BfuAI ACCTGC 1 cut(s) 799
BfuI GTATCC 1 cut(s) 1495
BmcAI AGTACT 1 cut(s) 1031
Bme1390I CCNGG 6 cut(s) 91, 207, 326, 706, 750, 1089
BmiI GGNNCC 5 cut(s) 330, 540, 865, 1126, 1341
BmrFI CCNGG 6 cut(s) 91, 207, 326, 706, 750, 1089
BmrI ACTGGG 1 cut(s) 327
BmsI GCATC 4 cut(s) 709, 1021, 1341, 1353
BmtI GCTAGC 1 cut(s) 8
BmuI ACTGGG 1 cut(s) 327
BpiI GAAGAC 2 cut(s) 57, 1726
BplI GAGNNNNNCTC 4 cut(s) 268, 300, 744, 776
BpmI CTGGAG 4 cut(s) 181, 771, 924, 1094
Bpu10I CCTNAGC 2 cut(s) 804, 998
BpuEI CTTGAG 1 cut(s) 992
BpuMI CCSGG 3 cut(s) 207, 326, 706
Bsa29I ATCGAT 1 cut(s) 1612
BsaAI YACGTR 2 cut(s) 380, 1300
BsaBI GATNNNNATC 1 cut(s) 417
BsaJI CCNNGG 7 cut(s) 90, 206, 324, 654, 704, 859, 1454
BsaWI WCCGGW 3 cut(s) 114, 568, 1342
BsaXI ACNNNNNCTCC 4 cut(s) 583, 613, 1402, 1432
Bsc4I CCNNNNNNNGG 7 cut(s) 21, 325, 683, 827, 1636, 1671, 1751
Bse1I ACTGG 4 cut(s) 228, 333, 907, 1337
Bse21I CCTNAGG 2 cut(s) 1533, 1550
Bse3DI GCAATG 4 cut(s) 695, 734, 839, 924
Bse8I GATNNNNATC 1 cut(s) 417
BseAI TCCGGA 1 cut(s) 1342
BseBI CCWGG 3 cut(s) 91, 750, 1089
BseCI ATCGAT 1 cut(s) 1612
BseDI CCNNGG 7 cut(s) 90, 206, 324, 654, 704, 859, 1454
BseGI GGATG 8 cut(s) 689, 700, 730, 1012, 1084, 1341, 1646, 1680
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 7 cut(s) 21, 325, 683, 827, 1636, 1671, 1751
BseMI GCAATG 4 cut(s) 695, 734, 839, 924
BseMII CTCAG 4 cut(s) 1012, 1350, 1524, 1569
BseNI ACTGG 4 cut(s) 228, 333, 907, 1337
BseRI GAGGAG 2 cut(s) 136, 591
BseSI GKGCMC 1 cut(s) 475
BseXI GCAGC 7 cut(s) 247, 511, 985, 1462, 1490, 1544, 1579
BseYI CCCAGC 2 cut(s) 816, 1629
BsgI GTGCAG 3 cut(s) 549, 777, 1046
Bsh1236I CGCG 2 cut(s) 134, 221
Bsh1285I CGRYCG 1 cut(s) 119
BshFI GGCC 5 cut(s) 89, 131, 227, 260, 858
BshNI GGYRCC 1 cut(s) 863
BshVI ATCGAT 1 cut(s) 1612
BsiEI CGRYCG 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 475
BsiSI CCGG 7 cut(s) 115, 206, 251, 326, 569, 706, 1343
BslFI GGGAC 6 cut(s) 315, 636, 651, 799, 1226, 1628
BslI CCNNNNNNNGG 7 cut(s) 21, 325, 683, 827, 1636, 1671, 1751
BsmAI GTCTC 5 cut(s) 159, 266, 345, 377, 1174
BsmBI CGTCTC 1 cut(s) 266
BsmFI GGGAC 6 cut(s) 315, 636, 651, 799, 1226, 1628
BsnI GGCC 5 cut(s) 89, 131, 227, 260, 858
Bsp1286I GDGCHC 2 cut(s) 475, 541
Bsp13I TCCGGA 1 cut(s) 1342
Bsp143I GATC 4 cut(s) 412, 429, 686, 1054
Bsp19I CCATGG 1 cut(s) 1454
BspACI CCGC 6 cut(s) 102, 132, 219, 233, 239, 1778
BspANI GGCC 5 cut(s) 89, 131, 227, 260, 858
BspCNI CTCAG 4 cut(s) 1011, 1351, 1525, 1570
BspDI ATCGAT 1 cut(s) 1612
BspEI TCCGGA 1 cut(s) 1342
BspFNI CGCG 2 cut(s) 134, 221
BspHI TCATGA 1 cut(s) 1111
BspLI GGNNCC 5 cut(s) 330, 540, 865, 1126, 1341
BspMAI CTGCAG 2 cut(s) 1480, 1597
BspMI ACCTGC 1 cut(s) 799
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 2 cut(s) 424, 694
BspT107I GGYRCC 1 cut(s) 863
BsrDI GCAATG 4 cut(s) 695, 734, 839, 924
BsrI ACTGG 4 cut(s) 228, 333, 907, 1337
BssECI CCNNGG 7 cut(s) 90, 206, 324, 654, 704, 859, 1454
BssMI GATC 4 cut(s) 412, 429, 686, 1054
BssT1I CCWWGG 1 cut(s) 1454
Bst2UI CCWGG 3 cut(s) 91, 750, 1089
Bst4CI ACNGT 4 cut(s) 176, 388, 650, 1121
BstBAI YACGTR 2 cut(s) 380, 1300
BstC8I GCNNGC 5 cut(s) 6, 225, 757, 1004, 1757
BstDEI CTNAG 6 cut(s) 804, 998, 1359, 1533, 1550, 1578
BstDSI CCRYGG 3 cut(s) 654, 859, 1454
BstF5I GGATG 8 cut(s) 689, 700, 730, 1012, 1084, 1341, 1646, 1680
BstFNI CGCG 2 cut(s) 134, 221
BstHHI GCGC 4 cut(s) 136, 203, 223, 491
BstKTI GATC 4 cut(s) 415, 432, 689, 1057
BstMAI GTCTC 5 cut(s) 159, 266, 345, 377, 1174
BstMBI GATC 4 cut(s) 412, 429, 686, 1054
BstMCI CGRYCG 1 cut(s) 119
BstMWI GCNNNNNNNGC 7 cut(s) 25, 247, 335, 355, 870, 914, 979
BstNI CCWGG 3 cut(s) 91, 750, 1089
BstNSI RCATGY 1 cut(s) 1759
BstSCI CCNGG 6 cut(s) 89, 205, 324, 704, 748, 1087
BstSFI CTRYAG 3 cut(s) 977, 1476, 1593
BstSLI GKGCMC 1 cut(s) 475
BstUI CGCG 2 cut(s) 134, 221
BstV1I GCAGC 7 cut(s) 247, 511, 985, 1462, 1490, 1544, 1579
BstV2I GAAGAC 2 cut(s) 57, 1726
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
Bsu15I ATCGAT 1 cut(s) 1612
Bsu36I CCTNAGG 2 cut(s) 1533, 1550
BsuI GTATCC 1 cut(s) 1495
BsuRI GGCC 5 cut(s) 89, 131, 227, 260, 858
BsuTUI ATCGAT 1 cut(s) 1612
BtgI CCRYGG 3 cut(s) 654, 859, 1454
BtsCI GGATG 8 cut(s) 689, 700, 730, 1012, 1084, 1341, 1646, 1680
BtsI GCAGTG 2 cut(s) 880, 1602
BtsIMutI CAGTG 6 cut(s) 340, 393, 880, 1117, 1602, 1733
BveI ACCTGC 1 cut(s) 799
Cac8I GCNNGC 5 cut(s) 6, 225, 757, 1004, 1757
CciI TCATGA 1 cut(s) 1111
CfoI GCGC 4 cut(s) 136, 203, 223, 491
ClaI ATCGAT 1 cut(s) 1612
Csp6I GTAC 4 cut(s) 377, 776, 1030, 1422
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviQI GTAC 4 cut(s) 377, 776, 1030, 1422
DdeI CTNAG 6 cut(s) 804, 998, 1359, 1533, 1550, 1578
DpnI GATC 4 cut(s) 414, 431, 688, 1056
DpnII GATC 4 cut(s) 412, 429, 686, 1054
DraI TTTAAA 1 cut(s) 1489
DraIII CACNNNGTG 1 cut(s) 773
DrdI GACNNNNNNGTC 1 cut(s) 391
DseDI GACNNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 3 cut(s) 225, 258, 856
Eco130I CCWWGG 1 cut(s) 1454
Eco24I GRGCYC 1 cut(s) 541
Eco57I CTGAAG 2 cut(s) 430, 744
Eco81I CCTNAGG 2 cut(s) 1533, 1550
EcoRII CCWGG 3 cut(s) 89, 748, 1087
EcoT14I CCWWGG 1 cut(s) 1454
EcoT22I ATGCAT 1 cut(s) 1368
EcoT38I GRGCYC 1 cut(s) 541
ErhI CCWWGG 1 cut(s) 1454
Esp3I CGTCTC 1 cut(s) 266
FalI AAGNNNNNCTT 2 cut(s) 748, 780
FaqI GGGAC 6 cut(s) 315, 636, 651, 799, 1226, 1628
FauI CCCGC 1 cut(s) 95
FokI GGATG 8 cut(s) 676, 687, 717, 999, 1071, 1348, 1633, 1687
FriOI GRGCYC 1 cut(s) 541
FspBI CTAG 8 cut(s) 5, 30, 396, 480, 498, 660, 771, 1746
FspI TGCGCA 1 cut(s) 490
GlaI GCGC 4 cut(s) 135, 202, 222, 490
GsaI CCCAGC 2 cut(s) 820, 1633
GsuI CTGGAG 4 cut(s) 181, 771, 924, 1094
HaeIII GGCC 5 cut(s) 89, 131, 227, 260, 858
HapII CCGG 7 cut(s) 115, 206, 251, 326, 569, 706, 1343
HhaI GCGC 4 cut(s) 136, 203, 223, 491
Hin6I GCGC 4 cut(s) 134, 201, 221, 489
HinP1I GCGC 4 cut(s) 134, 201, 221, 489
HincII GTYRAC 1 cut(s) 1587
HindII GTYRAC 1 cut(s) 1587
HindIII AAGCTT 1 cut(s) 848
HpaII CCGG 7 cut(s) 115, 206, 251, 326, 569, 706, 1343
HphI GGTGA 2 cut(s) 759, 774
Hpy166II GTNNAC 6 cut(s) 425, 473, 776, 1440, 1587, 1733
Hpy188I TCNGA 6 cut(s) 214, 616, 724, 1360, 1384, 1787
Hpy188III TCNNGA 8 cut(s) 410, 546, 941, 1073, 1112, 1293, 1343, 1517
Hpy8I GTNNAC 6 cut(s) 425, 473, 776, 1440, 1587, 1733
Hpy99I CGWCG 2 cut(s) 73, 76
HpyAV CCTTC 7 cut(s) 79, 121, 680, 774, 916, 1463, 1492
HpyCH4III ACNGT 4 cut(s) 176, 388, 650, 1121
HpyCH4IV ACGT 3 cut(s) 369, 379, 1299
HpyF10VI GCNNNNNNNGC 7 cut(s) 25, 247, 335, 355, 870, 914, 979
HpyF3I CTNAG 6 cut(s) 804, 998, 1359, 1533, 1550, 1578
HpySE526I ACGT 3 cut(s) 369, 379, 1299
HspAI GCGC 4 cut(s) 134, 201, 221, 489
Kpn2I TCCGGA 1 cut(s) 1342
Kzo9I GATC 4 cut(s) 412, 429, 686, 1054
LmnI GCTCC 9 cut(s) 179, 200, 334, 544, 752, 905, 1124, 1345, 1345
Lsp1109I GCAGC 7 cut(s) 247, 511, 985, 1462, 1490, 1544, 1579
LweI GCATC 4 cut(s) 709, 1021, 1341, 1353
MaeI CTAG 8 cut(s) 5, 30, 396, 480, 498, 660, 771, 1746
MaeII ACGT 3 cut(s) 369, 379, 1299
MaeIII GTNAC 1 cut(s) 715
MalI GATC 4 cut(s) 414, 431, 688, 1056
MboI GATC 4 cut(s) 412, 429, 686, 1054
MboII GAAGA 4 cut(s) 57, 415, 1064, 1731
MfeI CAATTG 1 cut(s) 1704
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 2 cut(s) 475, 541
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 7 cut(s) 154, 1044, 1228, 1543, 1678, 1704, 1760
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 4 cut(s) 586, 821, 1693, 1716
MmeI TCCRAC 1 cut(s) 192
Mox20I TGGCCA 1 cut(s) 227
Mph1103I ATGCAT 1 cut(s) 1368
MroI TCCGGA 1 cut(s) 1342
MroXI GAANNNNTTC 2 cut(s) 314, 936
MscI TGGCCA 1 cut(s) 227
MseI TTAA 5 cut(s) 825, 1098, 1488, 1620, 1763
MslI CAYNNNNRTG 2 cut(s) 14, 1754
Msp20I TGGCCA 1 cut(s) 227
MspI CCGG 7 cut(s) 115, 206, 251, 326, 569, 706, 1343
MspR9I CCNGG 6 cut(s) 91, 207, 326, 706, 750, 1089
MunI CAATTG 1 cut(s) 1704
MvaI CCWGG 3 cut(s) 91, 750, 1089
MvnI CGCG 2 cut(s) 134, 221
MwoI GCNNNNNNNGC 7 cut(s) 25, 247, 335, 355, 870, 914, 979
NciI CCSGG 3 cut(s) 207, 326, 706
NcoI CCATGG 1 cut(s) 1454
NdeII GATC 4 cut(s) 412, 429, 686, 1054
NheI GCTAGC 1 cut(s) 4
NlaIV GGNNCC 5 cut(s) 330, 540, 865, 1126, 1341
NmeAIII GCCGAG 1 cut(s) 293
NsbI TGCGCA 1 cut(s) 490
NsiI ATGCAT 1 cut(s) 1368
NspI RCATGY 1 cut(s) 1759
PaeI GCATGC 1 cut(s) 1759
PagI TCATGA 1 cut(s) 1111
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 74
PdmI GAANNNNTTC 2 cut(s) 314, 936
PfeI GAWTC 7 cut(s) 215, 254, 406, 501, 1241, 1306, 1741
PflFI GACNNNGTC 1 cut(s) 388
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 748
PleI GAGTC 4 cut(s) 586, 820, 1693, 1716
PpsI GAGTC 4 cut(s) 586, 820, 1693, 1716
Ppu21I YACGTR 2 cut(s) 380, 1300
Psp1406I AACGTT 1 cut(s) 369
Psp6I CCWGG 3 cut(s) 89, 748, 1087
PspFI CCCAGC 2 cut(s) 816, 1629
PspGI CCWGG 3 cut(s) 89, 748, 1087
PspN4I GGNNCC 5 cut(s) 330, 540, 865, 1126, 1341
PsrI GAACNNNNNNTAC 2 cut(s) 619, 651
PstI CTGCAG 2 cut(s) 1480, 1597
PsuI RGATCY 1 cut(s) 429
PsyI GACNNNGTC 1 cut(s) 388
RsaI GTAC 4 cut(s) 378, 777, 1031, 1423
RsaNI GTAC 4 cut(s) 377, 776, 1030, 1422
RseI CAYNNNNRTG 2 cut(s) 14, 1754
SaqAI TTAA 5 cut(s) 825, 1098, 1488, 1620, 1763
Sau3AI GATC 4 cut(s) 412, 429, 686, 1054
ScaI AGTACT 1 cut(s) 1031
SchI GAGTC 4 cut(s) 586, 821, 1693, 1716
ScrFI CCNGG 6 cut(s) 91, 207, 326, 706, 750, 1089
SduI GDGCHC 2 cut(s) 475, 541
SfaNI GCATC 4 cut(s) 709, 1021, 1341, 1353
SfcI CTRYAG 3 cut(s) 977, 1476, 1593
SmiMI CAYNNNNRTG 2 cut(s) 14, 1754
SmlI CTYRAG 1 cut(s) 1007
SmoI CTYRAG 1 cut(s) 1007
SpeI ACTAGT 1 cut(s) 479
SphI GCATGC 1 cut(s) 1759
Sse9I AATT 7 cut(s) 154, 1044, 1228, 1543, 1678, 1704, 1760
SsiI CCGC 6 cut(s) 102, 132, 219, 233, 239, 1778
SspI AATATT 1 cut(s) 1021
SspMI CTAG 8 cut(s) 5, 30, 396, 480, 498, 660, 771, 1746
StyD4I CCNGG 6 cut(s) 89, 205, 324, 704, 748, 1087
StyI CCWWGG 1 cut(s) 1454
TaaI ACNGT 4 cut(s) 176, 388, 650, 1121
TaiI ACGT 3 cut(s) 372, 382, 1302
TaqI TCGA 7 cut(s) 119, 140, 415, 453, 673, 1612, 1725
TasI AATT 7 cut(s) 154, 1044, 1228, 1543, 1678, 1704, 1760
TatI WGTACW 2 cut(s) 1029, 1421
TauI GCSGC 3 cut(s) 134, 241, 1781
TfiI GAWTC 7 cut(s) 215, 254, 406, 501, 1241, 1306, 1741
Tru1I TTAA 5 cut(s) 825, 1098, 1488, 1620, 1763
Tru9I TTAA 5 cut(s) 825, 1098, 1488, 1620, 1763
TscAI CASTG 6 cut(s) 340, 393, 880, 1124, 1602, 1740
TseI GCWGC 7 cut(s) 235, 524, 973, 1475, 1478, 1557, 1592
TspDTI ATGAA 9 cut(s) 645, 935, 1073, 1100, 1128, 1233, 1482, 1511, 1691
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 6 cut(s) 340, 393, 880, 1124, 1602, 1740
Tth111I GACNNNGTC 1 cut(s) 388
Van91I CCANNNNNTGG 1 cut(s) 21
VneI GTGCAC 1 cut(s) 471
XapI RAATTY 2 cut(s) 1543, 1678
XceI RCATGY 1 cut(s) 1759
XcmI CCANNNNNNNNNTGG 1 cut(s) 1169
XmnI GAANNNNTTC 2 cut(s) 314, 936
XspI CTAG 8 cut(s) 5, 30, 396, 480, 498, 660, 771, 1746
ZrmI AGTACT 1 cut(s) 1031
Zsp2I ATGCAT 1 cut(s) 1368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.