Rw2G013640

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001271
Physical Location & Seq
Forward (+)
14765620 .. 14768014
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013640.1

Sequence Viewer

Length: 981 bp
ATGACTCAACCTCCCCCTGCCTTATCAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTCCCAGGGGTAGAAGTCCCTTTCGACCCCTACAAGGATCATCCCCATTGCATCCCCGGTTATCTTCCCAGCCGATTAGAGGGCAATGGCAAAAGGGAAAGCTTATTGGTCGTGGCACGTTTGGCAGTGTTTATGTTGCTACAAATGGAGAAACTGGAGCTTTATGTGCAATGAAGGAAGTTGAACTACTTCCTGATGACCCGAAATCAGCAGAGTGTATAAAGCAGCTACAGCAGGAAATCAAAGTCCTCAGCCAGCTCAATCATCCTAATATTGTGCCGTACTATGGAAGGTTGAAGATAGGTTTTTTTATATATCTGGAGTATGTTCATCCTGGTTCTATTAACAAATATGTTCATGAACACTGTGGAGCCATGACAGAAGCTGTTGTTCGCAGTTTTACTCGCCATATAGTCTCTGGGTTGGCTTACTTGCACAGCACAAAAACAATACACAGGGACATCAAAGGGGCTAATTTGCTTCTTTCAGGACACGTAGGGAATCTTTCCCTAAAGGGAAGTCCATACTGGATGGCTCCAGAGCTGATGCAATCTGAGATGCATAAAGATAATAGCTCTGATTTGGCTCTTGCTGTTGATATATGGAGTTTGGGTTGTACTATTATTGAAATGTTCACAGGAAAACCACCATGGAGTGAGTATGAAGGGGCTGCAGCTATGTTTAAAGTTATGAAGGATACTCCACCATTTCCAGAAACATTGTCGCCTGAGGGTAAGGATTTCCTTAGGTGCTGCTTCCAAAGAAATCCTGCTGAGAGGTCAACTGCTGCAATGCTATTAGAACATCGATTTATAAAAAACTCCCAGCTGCAGGAAGTCCCATCTACGACCTATAACGCCTCTAATGGGATGAATTTCTTGGTAACTATAAAGGCCTTGCCATCCCACTTGTTTAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

326

Amino Acids

36.2

Weight (kDa)

8.65

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 50 - 292 1.5e-50 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 53 - 288 7.3e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 875
AclWI GGATC 1 cut(s) 106
AcsI RAATTY 1 cut(s) 934
AfaI GTAC 2 cut(s) 344, 679
AfiI CCNNNNNNNGG 5 cut(s) 95, 140, 347, 892, 927
AflIII ACRYGT 1 cut(s) 553
AgsI TTSAA 3 cut(s) 245, 358, 689
AjnI CCWGG 2 cut(s) 65, 394
AluBI AGCT 9 cut(s) 163, 221, 289, 319, 446, 604, 636, 737, 889
AluI AGCT 9 cut(s) 163, 221, 289, 319, 446, 604, 636, 737, 889
Alw26I GTCTC 1 cut(s) 481
AlwI GGATC 1 cut(s) 106
AlwNI CAGNNNCTG 1 cut(s) 446
AoxI GGCC 1 cut(s) 954
ApeKI GCWGC 6 cut(s) 286, 731, 734, 813, 848, 889
ApoI RAATTY 1 cut(s) 934
Asp700I GAANNNNTTC 1 cut(s) 249
AsuC2I CCSGG 1 cut(s) 118
AxyI CCTNAGG 2 cut(s) 789, 806
BarI GAAGNNNNNNTAC 2 cut(s) 231, 263
BbvCI CCTCAGC 1 cut(s) 311
BbvI GCAGC 6 cut(s) 298, 718, 746, 800, 835, 876
BccI CCATC 3 cut(s) 586, 910, 970
BceAI ACGGC 1 cut(s) 325
BciT130I CCWGG 2 cut(s) 67, 396
BciVI GTATCC 1 cut(s) 751
BcnI CCSGG 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 481
BfmI CTRYAG 3 cut(s) 290, 732, 890
BfuI GTATCC 1 cut(s) 751
BisI GCNGC 6 cut(s) 287, 732, 735, 814, 849, 890
BlsI GCNGC 6 cut(s) 288, 733, 736, 815, 850, 891
Bme1390I CCNGG 3 cut(s) 67, 118, 396
BmiI GGNNCC 2 cut(s) 433, 597
BmrFI CCNGG 3 cut(s) 67, 118, 396
BmsI GCATC 3 cut(s) 121, 597, 609
BpmI CTGGAG 3 cut(s) 237, 401, 582
Bpu10I CCTNAGC 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 118
Bsa29I ATCGAT 1 cut(s) 868
BsaAI YACGTR 1 cut(s) 556
BsaJI CCNNGG 4 cut(s) 65, 66, 116, 710
BsaXI ACNNNNNCTCC 3 cut(s) 25, 658, 688
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 140, 347, 892, 927
Bse1I ACTGG 2 cut(s) 220, 593
Bse21I CCTNAGG 2 cut(s) 789, 806
Bse3DI GCAATG 4 cut(s) 107, 152, 237, 858
BseBI CCWGG 2 cut(s) 67, 396
BseCI ATCGAT 1 cut(s) 868
BseDI CCNNGG 4 cut(s) 65, 66, 116, 710
BseGI GGATG 7 cut(s) 101, 112, 325, 391, 597, 936, 962
BseLI CCNNNNNNNGG 5 cut(s) 95, 140, 347, 892, 927
BseMI GCAATG 4 cut(s) 107, 152, 237, 858
BseMII CTCAG 4 cut(s) 325, 606, 780, 825
BseNI ACTGG 2 cut(s) 220, 593
BseXI GCAGC 6 cut(s) 298, 718, 746, 800, 835, 876
BseYI CCCAGC 2 cut(s) 129, 885
BshFI GGCC 1 cut(s) 956
BshVI ATCGAT 1 cut(s) 868
BsiSI CCGG 1 cut(s) 118
BslFI GGGAC 4 cut(s) 48, 63, 533, 884
BslI CCNNNNNNNGG 5 cut(s) 95, 140, 347, 892, 927
BsmAI GTCTC 1 cut(s) 481
BsmFI GGGAC 4 cut(s) 48, 63, 533, 884
BsnI GGCC 1 cut(s) 956
Bsp143I GATC 1 cut(s) 98
Bsp19I CCATGG 1 cut(s) 710
BspANI GGCC 1 cut(s) 956
BspCNI CTCAG 4 cut(s) 324, 607, 781, 826
BspDI ATCGAT 1 cut(s) 868
BspHI TCATGA 1 cut(s) 418
BspLI GGNNCC 2 cut(s) 433, 597
BspMAI CTGCAG 2 cut(s) 736, 894
BspPI GGATC 1 cut(s) 106
BsrDI GCAATG 4 cut(s) 107, 152, 237, 858
BsrI ACTGG 2 cut(s) 220, 593
BssECI CCNNGG 4 cut(s) 65, 66, 116, 710
BssMI GATC 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 710
Bst2UI CCWGG 2 cut(s) 67, 396
Bst4CI ACNGT 2 cut(s) 62, 428
BstBAI YACGTR 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 317
BstDEI CTNAG 5 cut(s) 311, 615, 789, 806, 834
BstDSI CCRYGG 1 cut(s) 710
BstF5I GGATG 7 cut(s) 101, 112, 325, 391, 597, 936, 962
BstKTI GATC 1 cut(s) 101
BstMAI GTCTC 1 cut(s) 481
BstMBI GATC 1 cut(s) 98
BstMWI GCNNNNNNNGC 3 cut(s) 183, 227, 292
BstNI CCWGG 2 cut(s) 67, 396
BstSCI CCNGG 3 cut(s) 65, 116, 394
BstSFI CTRYAG 3 cut(s) 290, 732, 890
BstV1I GCAGC 6 cut(s) 298, 718, 746, 800, 835, 876
Bsu15I ATCGAT 1 cut(s) 868
Bsu36I CCTNAGG 2 cut(s) 789, 806
BsuI GTATCC 1 cut(s) 751
BsuRI GGCC 1 cut(s) 956
BsuTUI ATCGAT 1 cut(s) 868
BtgI CCRYGG 1 cut(s) 710
BtsCI GGATG 7 cut(s) 101, 112, 325, 391, 597, 936, 962
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 2 cut(s) 193, 424
Cac8I GCNNGC 1 cut(s) 317
CaiI CAGNNNCTG 1 cut(s) 446
CciI TCATGA 1 cut(s) 418
ClaI ATCGAT 1 cut(s) 868
Csp6I GTAC 2 cut(s) 343, 678
CviAII CATG 3 cut(s) 419, 436, 711
CviQI GTAC 2 cut(s) 343, 678
DdeI CTNAG 5 cut(s) 311, 615, 789, 806, 834
DpnI GATC 1 cut(s) 100
DpnII GATC 1 cut(s) 98
DraI TTTAAA 1 cut(s) 745
Eco130I CCWWGG 1 cut(s) 710
Eco147I AGGCCT 1 cut(s) 956
Eco81I CCTNAGG 2 cut(s) 789, 806
EcoRII CCWGG 2 cut(s) 65, 394
EcoT14I CCWWGG 1 cut(s) 710
EcoT22I ATGCAT 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 710
FaeI CATG 3 cut(s) 422, 439, 714
FaqI GGGAC 4 cut(s) 48, 63, 533, 884
FatI CATG 3 cut(s) 418, 435, 710
Fnu4HI GCNGC 6 cut(s) 287, 732, 735, 814, 849, 890
FokI GGATG 7 cut(s) 88, 99, 312, 378, 604, 943, 949
Fsp4HI GCNGC 6 cut(s) 287, 732, 735, 814, 849, 890
GluI GCNGC 6 cut(s) 287, 732, 735, 814, 849, 890
GsaI CCCAGC 2 cut(s) 133, 889
GsuI CTGGAG 3 cut(s) 237, 401, 582
HaeIII GGCC 1 cut(s) 956
HapII CCGG 1 cut(s) 118
Hin1II CATG 3 cut(s) 422, 439, 714
HincII GTYRAC 1 cut(s) 843
HindII GTYRAC 1 cut(s) 843
HindIII AAGCTT 1 cut(s) 161
HinfI GANTC 2 cut(s) 4, 562
HpaII CCGG 1 cut(s) 118
Hpy166II GTNNAC 2 cut(s) 696, 843
Hpy188I TCNGA 3 cut(s) 28, 616, 640
Hpy188III TCNNGA 6 cut(s) 254, 380, 419, 549, 599, 773
Hpy8I GTNNAC 2 cut(s) 696, 843
HpyAV CCTTC 4 cut(s) 229, 345, 719, 748
HpyCH4III ACNGT 2 cut(s) 62, 428
HpyCH4IV ACGT 2 cut(s) 179, 555
HpyCH4V TGCA 8 cut(s) 112, 230, 496, 610, 622, 734, 851, 892
HpyF10VI GCNNNNNNNGC 3 cut(s) 183, 227, 292
HpyF3I CTNAG 5 cut(s) 311, 615, 789, 806, 834
HpySE526I ACGT 2 cut(s) 179, 555
Hsp92II CATG 3 cut(s) 422, 439, 714
Kzo9I GATC 1 cut(s) 98
LmnI GCTCC 3 cut(s) 218, 431, 601
Lsp1109I GCAGC 6 cut(s) 298, 718, 746, 800, 835, 876
LweI GCATC 3 cut(s) 121, 597, 609
MaeII ACGT 2 cut(s) 179, 555
MaeIII GTNAC 1 cut(s) 943
MalI GATC 1 cut(s) 100
MboI GATC 1 cut(s) 98
MboII GAAGA 2 cut(s) 117, 370
MluCI AATT 2 cut(s) 535, 934
MnlI CCTC 6 cut(s) 21, 134, 320, 784, 831, 931
Mph1103I ATGCAT 1 cut(s) 624
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 3 cut(s) 405, 744, 979
MspA1I CMGCKG 1 cut(s) 889
MspI CCGG 1 cut(s) 118
MspR9I CCNGG 3 cut(s) 67, 118, 396
MvaI CCWGG 2 cut(s) 67, 396
MwoI GCNNNNNNNGC 3 cut(s) 183, 227, 292
NciI CCSGG 1 cut(s) 118
NcoI CCATGG 1 cut(s) 710
NdeII GATC 1 cut(s) 98
NlaIII CATG 3 cut(s) 422, 439, 714
NlaIV GGNNCC 2 cut(s) 433, 597
NsiI ATGCAT 1 cut(s) 624
PagI TCATGA 1 cut(s) 418
PasI CCCWGGG 1 cut(s) 66
PceI AGGCCT 1 cut(s) 956
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 1 cut(s) 562
PkrI GCNGC 6 cut(s) 288, 733, 736, 815, 850, 891
Ppu21I YACGTR 1 cut(s) 556
PsiI TTATAA 1 cut(s) 875
Psp6I CCWGG 2 cut(s) 65, 394
PspFI CCCAGC 2 cut(s) 129, 885
PspGI CCWGG 2 cut(s) 65, 394
PspN4I GGNNCC 2 cut(s) 433, 597
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 2 cut(s) 736, 894
PstNI CAGNNNCTG 1 cut(s) 446
PvuII CAGCTG 1 cut(s) 889
RsaI GTAC 2 cut(s) 344, 679
RsaNI GTAC 2 cut(s) 343, 678
SaqAI TTAA 3 cut(s) 405, 744, 979
SatI GCNGC 6 cut(s) 287, 732, 735, 814, 849, 890
Sau3AI GATC 1 cut(s) 98
ScrFI CCNGG 3 cut(s) 67, 118, 396
SfaNI GCATC 3 cut(s) 121, 597, 609
SfcI CTRYAG 3 cut(s) 290, 732, 890
Sse9I AATT 2 cut(s) 535, 934
SseBI AGGCCT 1 cut(s) 956
SspI AATATT 1 cut(s) 334
StuI AGGCCT 1 cut(s) 956
StyD4I CCNGG 3 cut(s) 65, 116, 394
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 2 cut(s) 62, 428
TaiI ACGT 2 cut(s) 182, 558
TaqI TCGA 2 cut(s) 85, 868
TasI AATT 2 cut(s) 535, 934
TatI WGTACW 1 cut(s) 677
TfiI GAWTC 1 cut(s) 562
Tru1I TTAA 3 cut(s) 405, 744, 979
Tru9I TTAA 3 cut(s) 405, 744, 979
TscAI CASTG 2 cut(s) 193, 431
TseI GCWGC 6 cut(s) 286, 731, 734, 813, 848, 889
TspDTI ATGAA 8 cut(s) 57, 248, 380, 407, 435, 738, 767, 947
TspRI CASTG 2 cut(s) 193, 431
XapI RAATTY 1 cut(s) 934
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XmnI GAANNNNTTC 1 cut(s) 249
Zsp2I ATGCAT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.