Rh2AG173000

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001271
Physical Location & Seq
Forward (+)
16462631 .. 16466133
3503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG173000.1

Sequence Viewer

Length: 1479 bp
ATGCTAGCCACAACCATGCGTTGGTTGCCTAGCTTTGCTTCCTCATCATCATCTGCTTCGTCTTCGTCGTCGTCGTCGCCGAAAAAGAAGCCCAGGGAGGACGGCGGGTGGATTTTCCGGTCGAGGAGGAAGGCCGCGCAGTCGAGGAAACTGCTCAATTTGGGGAGAGACAATGACGGTGGAGCTATAACCAAATGCTCCAGCGCGCCGGGGTCGGAATCCGCGCTGGCCAGTCCGCAGCCGCTTCCTTTGCCGGAATCGGCCATCCGCCGAGAGACGAGGTGTTCTCGCTGTAAGTCATGGGGACATGAGACCTGCTTCCCCTCCCCGGCTCCCAGTGTGTTTTCTTGGCGAGACACTCCAAAGAATAACGTTCAAGTACGTGAGACAGTGTCGTCTAGGAGGAAGAATCAAGATCGAAATCGTTTACAGATCCCTGAACATGACTTCAGGCTCGATGTTCCTGTTAGAAGTGCACCCACTAGTCCATGCGCAAGTCCTAGATTCAGCCCACGCAGACCAAGTGCTGCTGATTTGTGGGCTCCGTCCAGAAATCAAGTCTGGTCTGCACCGGAGATGACTACTAATGATATGACTCAACCTCCCCCTGCCTTATCAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTCCCACGGCTAGAAGTCCCCTTCGACCCCCACAAGCATCATCCCCATTGCATCCCCGGTTATCTGTTACCTCTGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGCCTCCCTTCACCTAGTGTACCATCACCACAACCTGCACTCACACCTAAGCCAGAGTCCCAGCCGATTAAAGGGCAATGGCAAAAGGGAAAGCTTATTGGTCGTGGCACCTTTGGCAGTGTTTATGTTGCTACCAATAGGAAATCAAAGTCCTCAGCCAGCTCAAGCATCCTAATATTGTGCAGTACTATGGAAGTGAAATTGGACATCAAAGGGGCTAATTTGCTTGTGGATTCATGTGGGGTTGTCAAGCTGGCTGACTTTGGAATGGCCAAACATCTGATGCAATCTGAGATGCATAAAGATAACAGCTCTGATTTGGCTCTTGCTGTTGATATATGGAGTTTGGGTTGTACTATAATTGAAATGTTCACAGGAAAACCTCCATGGAGTGAGTATGAAGGGGCTGCAGCTATGTTTAAAGTTATGAAGGATACTCCACCATTTCCAGAAACATTGTCACCTGAGGGTAAGGACTTCCTTAGGTGCTGCTTCCAAAGAAATCCTGCTGAGAGGTCAACTGCTGCAATGCTATTAGAACATCGATTTATAAAAAACTCCCAGCTGCAGGAAGTCCCATCTACGACCTATAATGCCTCTAATGGGATGAATTTCTTGGAAAGACCCCAGACTCCAATTGAACTTGAAAGAAGACTTGATAGTTCACTGATGATTCCCAGCATTGGCATGCAATTAACTACGGGAAAGGCGGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

492

Amino Acids

53.74

Weight (kDa)

9.63

Isoelectric Point (pI)

68.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 321 - 437 4.4e-21 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 321 - 433 6.6e-10 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1310
AasI GACNNNNNNGTC 1 cut(s) 394
Acc16I TGCGCA 1 cut(s) 493
Acc36I ACCTGC 2 cut(s) 323, 802
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 21
AccII CGCG 3 cut(s) 137, 206, 224
AciI CCGC 7 cut(s) 105, 135, 222, 236, 242, 268, 1469
AclI AACGTT 1 cut(s) 372
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 3 cut(s) 228, 261, 1029
AcsI RAATTY 1 cut(s) 1369
AcuI CTGAAG 2 cut(s) 433, 747
AdeI CACNNNGTG 1 cut(s) 776
AfaI GTAC 4 cut(s) 381, 780, 946, 1114
AfiI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1327, 1362, 1442
AgsI TTSAA 4 cut(s) 377, 1124, 1400, 1406
AhlI ACTAGT 1 cut(s) 482
AjnI CCWGG 2 cut(s) 92, 751
AluBI AGCT 8 cut(s) 33, 185, 853, 921, 1012, 1071, 1172, 1324
AluI AGCT 8 cut(s) 33, 185, 853, 921, 1012, 1071, 1172, 1324
Alw21I GWGCWC 1 cut(s) 478
Alw26I GTCTC 5 cut(s) 162, 269, 305, 348, 380
Alw44I GTGCAC 1 cut(s) 474
AlwI GGATC 1 cut(s) 427
AoxI GGCC 4 cut(s) 132, 228, 261, 1029
ApaLI GTGCAC 1 cut(s) 474
ApeKI GCWGC 7 cut(s) 238, 527, 1166, 1169, 1248, 1283, 1324
ApoI RAATTY 1 cut(s) 1369
AspLEI GCGC 5 cut(s) 139, 206, 208, 226, 494
AsuC2I CCSGG 3 cut(s) 210, 329, 709
AsuHPI GGTGA 3 cut(s) 762, 777, 1212
AsuNHI GCTAGC 1 cut(s) 4
AxyI CCTNAGG 2 cut(s) 1224, 1241
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 2 cut(s) 230, 1031
BanI GGYRCC 1 cut(s) 866
BanII GRGCYC 1 cut(s) 544
BbsI GAAGAC 2 cut(s) 54, 1417
Bbv12I GWGCWC 1 cut(s) 478
BbvCI CCTCAGC 1 cut(s) 913
BbvI GCAGC 7 cut(s) 250, 514, 1153, 1181, 1235, 1270, 1311
BccI CCATC 4 cut(s) 272, 741, 790, 1345
BceAI ACGGC 2 cut(s) 118, 675
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 2 cut(s) 94, 753
BciVI GTATCC 1 cut(s) 1186
BcnI CCSGG 3 cut(s) 210, 329, 709
BcoDI GTCTC 5 cut(s) 162, 269, 305, 348, 380
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
BfmI CTRYAG 2 cut(s) 1167, 1325
BfuAI ACCTGC 2 cut(s) 323, 802
BfuI GTATCC 1 cut(s) 1186
BmcAI AGTACT 1 cut(s) 946
Bme1390I CCNGG 5 cut(s) 94, 210, 329, 709, 753
BmiI GGNNCC 3 cut(s) 333, 543, 868
BmrFI CCNGG 5 cut(s) 94, 210, 329, 709, 753
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 5 cut(s) 698, 712, 936, 1032, 1044
BmtI GCTAGC 1 cut(s) 8
BmuI ACTGGG 1 cut(s) 330
BpiI GAAGAC 2 cut(s) 54, 1417
BplI GAGNNNNNCTC 4 cut(s) 271, 303, 747, 779
BpmI CTGGAG 2 cut(s) 184, 774
Bpu10I CCTNAGC 2 cut(s) 807, 913
BpuEI CTTGAG 1 cut(s) 907
BpuMI CCSGG 3 cut(s) 210, 329, 709
Bsa29I ATCGAT 1 cut(s) 1303
BsaAI YACGTR 1 cut(s) 383
BsaBI GATNNNNATC 1 cut(s) 420
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 7 cut(s) 92, 93, 209, 327, 657, 707, 1145
BsaWI WCCGGW 2 cut(s) 117, 571
BsaXI ACNNNNNCTCC 4 cut(s) 586, 616, 1093, 1123
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1327, 1362, 1442
Bse1I ACTGG 2 cut(s) 231, 336
Bse21I CCTNAGG 2 cut(s) 1224, 1241
Bse3DI GCAATG 4 cut(s) 698, 737, 842, 1293
Bse8I GATNNNNATC 1 cut(s) 420
BseBI CCWGG 2 cut(s) 94, 753
BseCI ATCGAT 1 cut(s) 1303
BseDI CCNNGG 7 cut(s) 92, 93, 209, 327, 657, 707, 1145
BseGI GGATG 6 cut(s) 264, 692, 703, 733, 927, 1371
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1327, 1362, 1442
BseMI GCAATG 4 cut(s) 698, 737, 842, 1293
BseMII CTCAG 4 cut(s) 927, 1041, 1215, 1260
BseNI ACTGG 2 cut(s) 231, 336
BsePI GCGCGC 1 cut(s) 204
BseRI GAGGAG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 7 cut(s) 250, 514, 1153, 1181, 1235, 1270, 1311
BseYI CCCAGC 3 cut(s) 819, 1320, 1436
BsgI GTGCAG 3 cut(s) 552, 780, 961
Bsh1236I CGCG 3 cut(s) 137, 206, 224
Bsh1285I CGRYCG 1 cut(s) 122
BshFI GGCC 4 cut(s) 134, 230, 263, 1031
BshNI GGYRCC 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 1303
BsiEI CGRYCG 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 478
BsiSI CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
BslFI GGGAC 5 cut(s) 318, 639, 654, 802, 1319
BslI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1327, 1362, 1442
BsmAI GTCTC 5 cut(s) 162, 269, 305, 348, 380
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 5 cut(s) 318, 639, 654, 802, 1319
BsnI GGCC 4 cut(s) 134, 230, 263, 1031
Bso31I GGTCTC 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 478, 544
Bsp143I GATC 2 cut(s) 415, 432
Bsp19I CCATGG 1 cut(s) 1145
BspACI CCGC 7 cut(s) 105, 135, 222, 236, 242, 268, 1469
BspANI GGCC 4 cut(s) 134, 230, 263, 1031
BspCNI CTCAG 4 cut(s) 926, 1042, 1216, 1261
BspDI ATCGAT 1 cut(s) 1303
BspFNI CGCG 3 cut(s) 137, 206, 224
BspLI GGNNCC 3 cut(s) 333, 543, 868
BspMAI CTGCAG 2 cut(s) 1171, 1329
BspMI ACCTGC 2 cut(s) 323, 802
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 427
BspT107I GGYRCC 1 cut(s) 866
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 4 cut(s) 698, 737, 842, 1293
BsrI ACTGG 2 cut(s) 231, 336
BssECI CCNNGG 7 cut(s) 92, 93, 209, 327, 657, 707, 1145
BssHII GCGCGC 1 cut(s) 204
BssMI GATC 2 cut(s) 415, 432
BssT1I CCWWGG 1 cut(s) 1145
Bst2UI CCWGG 2 cut(s) 94, 753
Bst4CI ACNGT 3 cut(s) 179, 391, 653
BstBAI YACGTR 1 cut(s) 383
BstC8I GCNNGC 7 cut(s) 6, 206, 228, 760, 919, 1014, 1448
BstDEI CTNAG 6 cut(s) 807, 913, 1050, 1224, 1241, 1269
BstDSI CCRYGG 2 cut(s) 657, 1145
BstF5I GGATG 6 cut(s) 264, 692, 703, 733, 927, 1371
BstFNI CGCG 3 cut(s) 137, 206, 224
BstHHI GCGC 5 cut(s) 139, 206, 208, 226, 494
BstKTI GATC 2 cut(s) 418, 435
BstMAI GTCTC 5 cut(s) 162, 269, 305, 348, 380
BstMBI GATC 2 cut(s) 415, 432
BstMCI CGRYCG 1 cut(s) 122
BstMWI GCNNNNNNNGC 3 cut(s) 25, 250, 873
BstNI CCWGG 2 cut(s) 94, 753
BstNSI RCATGY 1 cut(s) 1450
BstSCI CCNGG 5 cut(s) 92, 208, 327, 707, 751
BstSFI CTRYAG 2 cut(s) 1167, 1325
BstSLI GKGCMC 1 cut(s) 478
BstUI CGCG 3 cut(s) 137, 206, 224
BstV1I GCAGC 7 cut(s) 250, 514, 1153, 1181, 1235, 1270, 1311
BstV2I GAAGAC 2 cut(s) 54, 1417
BstX2I RGATCY 1 cut(s) 432
BstYI RGATCY 1 cut(s) 432
Bsu15I ATCGAT 1 cut(s) 1303
Bsu36I CCTNAGG 2 cut(s) 1224, 1241
BsuI GTATCC 1 cut(s) 1186
BsuRI GGCC 4 cut(s) 134, 230, 263, 1031
BsuTUI ATCGAT 1 cut(s) 1303
BtgI CCRYGG 2 cut(s) 657, 1145
BtsCI GGATG 6 cut(s) 264, 692, 703, 733, 927, 1371
BtsI GCAGTG 1 cut(s) 883
BtsIMutI CAGTG 4 cut(s) 343, 396, 883, 1424
BveI ACCTGC 2 cut(s) 323, 802
Cac8I GCNNGC 7 cut(s) 6, 206, 228, 760, 919, 1014, 1448
CfoI GCGC 5 cut(s) 139, 206, 208, 226, 494
ClaI ATCGAT 1 cut(s) 1303
Csp6I GTAC 4 cut(s) 380, 779, 945, 1113
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 8 cut(s) 16, 300, 308, 443, 489, 996, 1146, 1447
CviQI GTAC 4 cut(s) 380, 779, 945, 1113
DdeI CTNAG 6 cut(s) 807, 913, 1050, 1224, 1241, 1269
DpnI GATC 2 cut(s) 417, 434
DpnII GATC 2 cut(s) 415, 432
DraI TTTAAA 1 cut(s) 1180
DraIII CACNNNGTG 1 cut(s) 776
DrdI GACNNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 3 cut(s) 228, 261, 1029
EciI GGCGGA 1 cut(s) 257
Eco130I CCWWGG 1 cut(s) 1145
Eco24I GRGCYC 1 cut(s) 544
Eco31I GGTCTC 1 cut(s) 305
Eco57I CTGAAG 2 cut(s) 433, 747
Eco81I CCTNAGG 2 cut(s) 1224, 1241
EcoRII CCWGG 2 cut(s) 92, 751
EcoT14I CCWWGG 1 cut(s) 1145
EcoT22I ATGCAT 1 cut(s) 1059
EcoT38I GRGCYC 1 cut(s) 544
ErhI CCWWGG 1 cut(s) 1145
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 8 cut(s) 19, 303, 311, 446, 492, 999, 1149, 1450
FalI AAGNNNNNCTT 2 cut(s) 751, 783
FaqI GGGAC 5 cut(s) 318, 639, 654, 802, 1319
FatI CATG 8 cut(s) 15, 299, 307, 442, 488, 995, 1145, 1446
FauI CCCGC 1 cut(s) 98
FokI GGATG 6 cut(s) 251, 679, 690, 720, 914, 1378
FriOI GRGCYC 1 cut(s) 544
FspBI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
FspI TGCGCA 1 cut(s) 493
GlaI GCGC 5 cut(s) 138, 205, 207, 225, 493
GsaI CCCAGC 3 cut(s) 823, 1324, 1440
GsuI CTGGAG 2 cut(s) 184, 774
HaeIII GGCC 4 cut(s) 134, 230, 263, 1031
HapII CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
HhaI GCGC 5 cut(s) 139, 206, 208, 226, 494
Hin1II CATG 8 cut(s) 19, 303, 311, 446, 492, 999, 1149, 1450
Hin6I GCGC 5 cut(s) 137, 204, 206, 224, 492
HinP1I GCGC 5 cut(s) 137, 204, 206, 224, 492
HincII GTYRAC 1 cut(s) 1278
HindII GTYRAC 1 cut(s) 1278
HindIII AAGCTT 1 cut(s) 851
HinfI GANTC 9 cut(s) 218, 257, 409, 504, 595, 815, 992, 1390, 1432
HpaII CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
HphI GGTGA 3 cut(s) 762, 777, 1212
Hpy166II GTNNAC 6 cut(s) 428, 476, 779, 1131, 1278, 1424
Hpy188I TCNGA 7 cut(s) 217, 619, 727, 1041, 1051, 1075, 1478
Hpy188III TCNNGA 3 cut(s) 413, 549, 1208
Hpy8I GTNNAC 6 cut(s) 428, 476, 779, 1131, 1278, 1424
Hpy99I CGWCG 4 cut(s) 70, 73, 76, 79
HpyAV CCTTC 5 cut(s) 124, 683, 777, 1154, 1183
HpyCH4III ACNGT 3 cut(s) 179, 391, 653
HpyCH4IV ACGT 2 cut(s) 372, 382
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 250, 873
HpyF3I CTNAG 6 cut(s) 807, 913, 1050, 1224, 1241, 1269
HpySE526I ACGT 2 cut(s) 372, 382
Hsp92II CATG 8 cut(s) 19, 303, 311, 446, 492, 999, 1149, 1450
HspAI GCGC 5 cut(s) 137, 204, 206, 224, 492
Kzo9I GATC 2 cut(s) 415, 432
LmnI GCTCC 5 cut(s) 182, 203, 337, 547, 755
Lsp1109I GCAGC 7 cut(s) 250, 514, 1153, 1181, 1235, 1270, 1311
LweI GCATC 5 cut(s) 698, 712, 936, 1032, 1044
MaeI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
MaeII ACGT 2 cut(s) 372, 382
MaeIII GTNAC 2 cut(s) 718, 1218
MalI GATC 2 cut(s) 417, 434
MboI GATC 2 cut(s) 415, 432
MboII GAAGA 3 cut(s) 54, 418, 1422
MfeI CAATTG 1 cut(s) 1395
MflI RGATCY 1 cut(s) 432
MhlI GDGCHC 2 cut(s) 478, 544
MlsI TGGCCA 2 cut(s) 230, 1031
MluCI AATT 7 cut(s) 157, 959, 979, 1119, 1369, 1395, 1451
MluNI TGGCCA 2 cut(s) 230, 1031
MlyI GAGTC 3 cut(s) 589, 824, 1384
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 2 cut(s) 230, 1031
Mph1103I ATGCAT 1 cut(s) 1059
MscI TGGCCA 2 cut(s) 230, 1031
MseI TTAA 3 cut(s) 828, 1179, 1454
MslI CAYNNNNRTG 2 cut(s) 14, 1445
Msp20I TGGCCA 2 cut(s) 230, 1031
MspA1I CMGCKG 1 cut(s) 1324
MspI CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
MspR9I CCNGG 5 cut(s) 94, 210, 329, 709, 753
MunI CAATTG 1 cut(s) 1395
MvaI CCWGG 2 cut(s) 94, 753
MvnI CGCG 3 cut(s) 137, 206, 224
MwoI GCNNNNNNNGC 3 cut(s) 25, 250, 873
NciI CCSGG 3 cut(s) 210, 329, 709
NcoI CCATGG 1 cut(s) 1145
NdeII GATC 2 cut(s) 415, 432
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 8 cut(s) 19, 303, 311, 446, 492, 999, 1149, 1450
NlaIV GGNNCC 3 cut(s) 333, 543, 868
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 1218
NsbI TGCGCA 1 cut(s) 493
NsiI ATGCAT 1 cut(s) 1059
NspI RCATGY 1 cut(s) 1450
PaeI GCATGC 1 cut(s) 1450
PasI CCCWGGG 1 cut(s) 93
PauI GCGCGC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 3 cut(s) 65, 71, 77
PfeI GAWTC 6 cut(s) 218, 257, 409, 504, 992, 1432
PflFI GACNNNGTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 751
PleI GAGTC 3 cut(s) 589, 823, 1384
PpsI GAGTC 3 cut(s) 589, 823, 1384
Ppu21I YACGTR 1 cut(s) 383
PsiI TTATAA 1 cut(s) 1310
Psp1406I AACGTT 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 92, 751
PspFI CCCAGC 3 cut(s) 819, 1320, 1436
PspGI CCWGG 2 cut(s) 92, 751
PspN4I GGNNCC 3 cut(s) 333, 543, 868
PsrI GAACNNNNNNTAC 2 cut(s) 622, 654
PstI CTGCAG 2 cut(s) 1171, 1329
PsuI RGATCY 1 cut(s) 432
PsyI GACNNNGTC 1 cut(s) 391
PteI GCGCGC 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 1324
RsaI GTAC 4 cut(s) 381, 780, 946, 1114
RsaNI GTAC 4 cut(s) 380, 779, 945, 1113
RseI CAYNNNNRTG 2 cut(s) 14, 1445
SaqAI TTAA 3 cut(s) 828, 1179, 1454
Sau3AI GATC 2 cut(s) 415, 432
ScaI AGTACT 1 cut(s) 946
SchI GAGTC 3 cut(s) 589, 824, 1384
ScrFI CCNGG 5 cut(s) 94, 210, 329, 709, 753
SduI GDGCHC 2 cut(s) 478, 544
SfaNI GCATC 5 cut(s) 698, 712, 936, 1032, 1044
SfcI CTRYAG 2 cut(s) 1167, 1325
SmiMI CAYNNNNRTG 2 cut(s) 14, 1445
SmlI CTYRAG 1 cut(s) 922
SmoI CTYRAG 1 cut(s) 922
SpeI ACTAGT 1 cut(s) 482
SphI GCATGC 1 cut(s) 1450
Sse9I AATT 7 cut(s) 157, 959, 979, 1119, 1369, 1395, 1451
SsiI CCGC 7 cut(s) 105, 135, 222, 236, 242, 268, 1469
SspI AATATT 1 cut(s) 936
SspMI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
StyD4I CCNGG 5 cut(s) 92, 208, 327, 707, 751
StyI CCWWGG 1 cut(s) 1145
TaaI ACNGT 3 cut(s) 179, 391, 653
TaiI ACGT 2 cut(s) 375, 385
TaqI TCGA 6 cut(s) 122, 143, 418, 456, 676, 1303
TasI AATT 7 cut(s) 157, 959, 979, 1119, 1369, 1395, 1451
TatI WGTACW 2 cut(s) 944, 1112
TauI GCSGC 3 cut(s) 137, 244, 1472
TfiI GAWTC 6 cut(s) 218, 257, 409, 504, 992, 1432
Tru1I TTAA 3 cut(s) 828, 1179, 1454
Tru9I TTAA 3 cut(s) 828, 1179, 1454
TscAI CASTG 4 cut(s) 343, 396, 883, 1431
TseFI GTSAC 1 cut(s) 1218
TseI GCWGC 7 cut(s) 238, 527, 1166, 1169, 1248, 1283, 1324
Tsp45I GTSAC 1 cut(s) 1218
TspDTI ATGAA 5 cut(s) 648, 984, 1173, 1202, 1382
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 4 cut(s) 343, 396, 883, 1431
Tth111I GACNNNGTC 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 21
VneI GTGCAC 1 cut(s) 474
XapI RAATTY 1 cut(s) 1369
XceI RCATGY 1 cut(s) 1450
XspI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
ZrmI AGTACT 1 cut(s) 946
Zsp2I ATGCAT 1 cut(s) 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.