pycom15g03320

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001271
Physical Location & Seq
Forward (+)
2059224 .. 2060457
1234 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g03320.4

Sequence Viewer

Length: 903 bp
ATGTCTTCTTCTACTTTCTTCTGTGTTTCAGGTTCGAAACGGTGCAGTGGTGGCGGCTGCTGTGAGCAGAGCCCAAGGCGGAAGCTCCACCACAGCAACCCGGAATTGGGTTCTCCCGCCTCGGCCACTTTCCCTGAATTTTCAATCGGAAAGTCACATTCTCTGGATTGGGGTGTTGCTGGTGCTGCTGGTGCTGCTCCACAACCCTTGCCCCTTCCAGAATCTTCCCTGATTCAGAGACCGGACGCGGTTCCTAAACAAGGGCATGACGAAAATCAAGTTGCCACAGCCTCCATATCAGGACCGCTGACTTCTTACTCAGGCTGCATTCATCCAGATGAAAATTCTTGTGGGTGTTATTTTAACTTTTGTCTCCCGACGAAAACTCAGGCAAATGATATCTCAAGCCATACAGTTAGCCCCCGAAGAACAAATGCGGCCGCAGATCCTTTACCTTCTTTTGGGGGTCCCCAAGATCACTATCAGGATTCATTCCCTTCGAGGTTATCACCAAAGGGACAATCTCGTAGTCTTGGTCATTCTCCTCTTCACAGCTCCCCATCTCGAAGCCAACGCTTCAACCCAAAAGGTTCAAATGGAACTGCAGTCACAGAGAGTTGTTCTGAGAGGGTTGAAAGTAGTCATCGCGTCAATGCTCACCCTTTGCCTCTGCCTCCTCCACCACAGTCATCTATCATGCATAACAATGCCGAAATTTTACCAGTATCATCTATTAAAGGACAATGGAAGAAGGGAAAACTTATTGGCAGGGGTACCTTAGGCAGTGTTTATCTCGCAACCAACCGAGAGACCGGGGCATTGTGCGCAATGAAGGAAGTTGATCTCATTCCTGATCGATCCGAAATCTGCAGAATGTGTAAAGCAATTGGAGCAGGAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

301

Amino Acids

31.75

Weight (kDa)

8.73

Isoelectric Point (pI)

65.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 826
Acc65I GGTACC 1 cut(s) 773
AccB1I GGYRCC 1 cut(s) 773
AccII CGCG 2 cut(s) 248, 648
AciI CCGC 7 cut(s) 54, 79, 117, 248, 305, 437, 441
AclWI GGATC 2 cut(s) 440, 852
AcoI YGGCCR 2 cut(s) 123, 438
AcsI RAATTY 3 cut(s) 137, 343, 714
AfaI GTAC 1 cut(s) 775
AfiI CCNNNNNNNGG 4 cut(s) 106, 107, 260, 461
AgsI TTSAA 4 cut(s) 144, 580, 594, 635
AluBI AGCT 2 cut(s) 85, 555
AluI AGCT 2 cut(s) 85, 555
Alw26I GTCTC 3 cut(s) 232, 377, 803
AlwI GGATC 2 cut(s) 440, 852
AoxI GGCC 2 cut(s) 123, 438
ApeKI GCWGC 4 cut(s) 57, 185, 194, 324
ApoI RAATTY 3 cut(s) 137, 343, 714
Asp718I GGTACC 1 cut(s) 773
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 2 cut(s) 302, 467
AsuC2I CCSGG 2 cut(s) 101, 814
AsuHPI GGTGA 2 cut(s) 501, 650
AsuII TTCGAA 1 cut(s) 35
AvaII GGWCC 2 cut(s) 302, 467
AxyI CCTNAGG 1 cut(s) 778
BanI GGYRCC 1 cut(s) 773
BanII GRGCYC 1 cut(s) 74
BbvI GCAGC 4 cut(s) 44, 172, 181, 311
BccI CCATC 1 cut(s) 568
BcnI CCSGG 2 cut(s) 101, 814
BcoDI GTCTC 3 cut(s) 232, 377, 803
BfmI CTRYAG 2 cut(s) 603, 868
BisI GCNGC 7 cut(s) 55, 58, 186, 195, 325, 438, 441
BlsI GCNGC 7 cut(s) 56, 59, 187, 196, 326, 439, 442
Bme1390I CCNGG 2 cut(s) 101, 814
Bme18I GGWCC 2 cut(s) 302, 467
BmgT120I GGNCC 2 cut(s) 302, 467
BmiI GGNNCC 4 cut(s) 252, 468, 469, 775
BmrFI CCNGG 2 cut(s) 101, 814
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 1 cut(s) 388
BpuMI CCSGG 2 cut(s) 101, 814
Bsa29I ATCGAT 1 cut(s) 856
BsaBI GATNNNNATC 1 cut(s) 480
BsaI GGTCTC 2 cut(s) 232, 803
BsaJI CCNNGG 3 cut(s) 74, 120, 813
BsaWI WCCGGW 1 cut(s) 241
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 107, 260, 461
Bse1I ACTGG 1 cut(s) 722
Bse21I CCTNAGG 1 cut(s) 778
Bse3DI GCAATG 1 cut(s) 834
Bse8I GATNNNNATC 1 cut(s) 480
BseCI ATCGAT 1 cut(s) 856
BseDI CCNNGG 3 cut(s) 74, 120, 813
BseGI GGATG 1 cut(s) 331
BseJI GATNNNNATC 1 cut(s) 480
BseLI CCNNNNNNNGG 4 cut(s) 106, 107, 260, 461
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 3 cut(s) 333, 401, 615
BseNI ACTGG 1 cut(s) 722
BseRI GAGGAG 2 cut(s) 534, 666
BseX3I CGGCCG 1 cut(s) 438
BseXI GCAGC 4 cut(s) 44, 172, 181, 311
BsgI GTGCAG 1 cut(s) 64
Bsh1236I CGCG 2 cut(s) 248, 648
Bsh1285I CGRYCG 1 cut(s) 441
BshFI GGCC 2 cut(s) 125, 440
BshNI GGYRCC 1 cut(s) 773
BshVI ATCGAT 1 cut(s) 856
BsiEI CGRYCG 1 cut(s) 441
BsiSI CCGG 3 cut(s) 101, 242, 813
BslFI GGGAC 2 cut(s) 453, 531
BslI CCNNNNNNNGG 4 cut(s) 106, 107, 260, 461
BsmAI GTCTC 3 cut(s) 232, 377, 803
BsmFI GGGAC 2 cut(s) 453, 531
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 2 cut(s) 125, 440
Bso31I GGTCTC 2 cut(s) 232, 803
Bsp119I TTCGAA 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 74
Bsp143I GATC 5 cut(s) 445, 475, 841, 853, 857
BspACI CCGC 7 cut(s) 54, 79, 117, 248, 305, 437, 441
BspANI GGCC 2 cut(s) 125, 440
BspCNI CTCAG 3 cut(s) 332, 400, 616
BspDI ATCGAT 1 cut(s) 856
BspFNI CGCG 2 cut(s) 248, 648
BspLI GGNNCC 4 cut(s) 252, 468, 469, 775
BspMAI CTGCAG 2 cut(s) 607, 872
BspPI GGATC 2 cut(s) 440, 852
BspT104I TTCGAA 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 773
BspTNI GGTCTC 2 cut(s) 232, 803
BsrDI GCAATG 1 cut(s) 834
BsrI ACTGG 1 cut(s) 722
BssECI CCNNGG 3 cut(s) 74, 120, 813
BssMI GATC 5 cut(s) 445, 475, 841, 853, 857
BssT1I CCWWGG 1 cut(s) 74
Bst4CI ACNGT 3 cut(s) 42, 415, 687
Bst6I CTCTTC 1 cut(s) 552
BstBI TTCGAA 1 cut(s) 35
BstDEI CTNAG 4 cut(s) 319, 387, 624, 778
BstENI CCTNNNNNAGG 1 cut(s) 258
BstF5I GGATG 1 cut(s) 331
BstFNI CGCG 2 cut(s) 248, 648
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 5 cut(s) 448, 478, 844, 856, 860
BstMAI GTCTC 3 cut(s) 232, 377, 803
BstMBI GATC 5 cut(s) 445, 475, 841, 853, 857
BstMCI CGRYCG 1 cut(s) 441
BstMWI GCNNNNNNNGC 6 cut(s) 51, 185, 191, 194, 824, 890
BstSCI CCNGG 2 cut(s) 99, 812
BstSFI CTRYAG 2 cut(s) 603, 868
BstUI CGCG 2 cut(s) 248, 648
BstV1I GCAGC 4 cut(s) 44, 172, 181, 311
BstX2I RGATCY 1 cut(s) 445
BstYI RGATCY 1 cut(s) 445
BstZI CGGCCG 1 cut(s) 438
Bsu15I ATCGAT 1 cut(s) 856
Bsu36I CCTNAGG 1 cut(s) 778
BsuRI GGCC 2 cut(s) 125, 440
BsuTUI ATCGAT 1 cut(s) 856
BtgZI GCGATG 1 cut(s) 629
BtsCI GGATG 1 cut(s) 331
BtsI GCAGTG 2 cut(s) 52, 790
BtsIMutI CAGTG 2 cut(s) 52, 790
CciNI GCGGCCGC 1 cut(s) 438
CfoI GCGC 1 cut(s) 827
Cfr13I GGNCC 2 cut(s) 302, 467
ClaI ATCGAT 1 cut(s) 856
CseI GACGC 2 cut(s) 254, 637
Csp6I GTAC 1 cut(s) 774
CspCI CAANNNNNGTGG 2 cut(s) 189, 224
CviAII CATG 2 cut(s) 266, 697
CviQI GTAC 1 cut(s) 774
DdeI CTNAG 4 cut(s) 319, 387, 624, 778
DpnI GATC 5 cut(s) 447, 477, 843, 855, 859
DpnII GATC 5 cut(s) 445, 475, 841, 853, 857
EaeI YGGCCR 2 cut(s) 123, 438
EagI CGGCCG 1 cut(s) 438
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
EciI GGCGGA 1 cut(s) 94
EclXI CGGCCG 1 cut(s) 438
Eco130I CCWWGG 1 cut(s) 74
Eco24I GRGCYC 1 cut(s) 74
Eco31I GGTCTC 2 cut(s) 232, 803
Eco32I GATATC 1 cut(s) 400
Eco47I GGWCC 2 cut(s) 302, 467
Eco52I CGGCCG 1 cut(s) 438
Eco81I CCTNAGG 1 cut(s) 778
EcoNI CCTNNNNNAGG 1 cut(s) 258
EcoO109I RGGNCCY 1 cut(s) 467
EcoRV GATATC 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 74
EcoT22I ATGCAT 1 cut(s) 702
EcoT38I GRGCYC 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 74
FaeI CATG 2 cut(s) 269, 700
FaiI YATR 5 cut(s) 267, 296, 411, 698, 702
FaqI GGGAC 2 cut(s) 453, 531
FatI CATG 2 cut(s) 265, 696
FauI CCCGC 1 cut(s) 124
Fnu4HI GCNGC 7 cut(s) 55, 58, 186, 195, 325, 438, 441
FokI GGATG 1 cut(s) 318
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 7 cut(s) 55, 58, 186, 195, 325, 438, 441
FspI TGCGCA 1 cut(s) 826
GlaI GCGC 1 cut(s) 826
GluI GCNGC 7 cut(s) 55, 58, 186, 195, 325, 438, 441
HaeIII GGCC 2 cut(s) 125, 440
HapII CCGG 3 cut(s) 101, 242, 813
HgaI GACGC 2 cut(s) 254, 637
HhaI GCGC 1 cut(s) 827
Hin1II CATG 2 cut(s) 269, 700
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HinfI GANTC 3 cut(s) 221, 232, 488
HpaII CCGG 3 cut(s) 101, 242, 813
HphI GGTGA 2 cut(s) 501, 650
Hpy188I TCNGA 4 cut(s) 149, 237, 625, 862
Hpy188III TCNNGA 8 cut(s) 164, 218, 300, 335, 376, 485, 564, 851
Hpy99I CGWCG 1 cut(s) 382
HpyAV CCTTC 5 cut(s) 224, 465, 507, 745, 826
HpyCH4III ACNGT 3 cut(s) 42, 415, 687
HpyCH4V TGCA 5 cut(s) 45, 327, 605, 700, 870
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 185, 191, 194, 824, 890
HpyF3I CTNAG 4 cut(s) 319, 387, 624, 778
Hsp92II CATG 2 cut(s) 269, 700
HspAI GCGC 1 cut(s) 825
KflI GGGWCCC 1 cut(s) 467
KpnI GGTACC 1 cut(s) 777
Kzo9I GATC 5 cut(s) 445, 475, 841, 853, 857
LmnI GCTCC 4 cut(s) 90, 202, 560, 890
Lsp1109I GCAGC 4 cut(s) 44, 172, 181, 311
MaeIII GTNAC 2 cut(s) 153, 607
MalI GATC 5 cut(s) 447, 477, 843, 855, 859
MboI GATC 5 cut(s) 445, 475, 841, 853, 857
MboII GAAGA 5 cut(s) 10, 216, 438, 539, 760
MfeI CAATTG 1 cut(s) 885
MflI RGATCY 1 cut(s) 445
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 6 cut(s) 104, 137, 343, 714, 885, 898
MnlI CCTC 8 cut(s) 130, 301, 495, 555, 621, 678, 684, 687
Mph1103I ATGCAT 1 cut(s) 702
MseI TTAA 3 cut(s) 363, 735, 901
MslI CAYNNNNRTG 2 cut(s) 336, 705
MspA1I CMGCKG 1 cut(s) 307
MspI CCGG 3 cut(s) 101, 242, 813
MspR9I CCNGG 2 cut(s) 101, 814
MunI CAATTG 1 cut(s) 885
Mva1269I GAATGC 1 cut(s) 327
MvnI CGCG 2 cut(s) 248, 648
MwoI GCNNNNNNNGC 6 cut(s) 51, 185, 191, 194, 824, 890
NciI CCSGG 2 cut(s) 101, 814
NdeII GATC 5 cut(s) 445, 475, 841, 853, 857
NlaIII CATG 2 cut(s) 269, 700
NlaIV GGNNCC 4 cut(s) 252, 468, 469, 775
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 2 cut(s) 153, 607
NotI GCGGCCGC 1 cut(s) 438
NsbI TGCGCA 1 cut(s) 826
NsiI ATGCAT 1 cut(s) 702
NspV TTCGAA 1 cut(s) 35
PctI GAATGC 1 cut(s) 327
PfeI GAWTC 3 cut(s) 221, 232, 488
PkrI GCNGC 7 cut(s) 56, 59, 187, 196, 326, 439, 442
PpuMI RGGWCCY 1 cut(s) 467
Psp5II RGGWCCY 1 cut(s) 467
PspN4I GGNNCC 4 cut(s) 252, 468, 469, 775
PspPI GGNCC 2 cut(s) 302, 467
PspPPI RGGWCCY 1 cut(s) 467
PstI CTGCAG 2 cut(s) 607, 872
PsuI RGATCY 1 cut(s) 445
RsaI GTAC 1 cut(s) 775
RsaNI GTAC 1 cut(s) 774
RseI CAYNNNNRTG 2 cut(s) 336, 705
SaqAI TTAA 3 cut(s) 363, 735, 901
SatI GCNGC 7 cut(s) 55, 58, 186, 195, 325, 438, 441
Sau3AI GATC 5 cut(s) 445, 475, 841, 853, 857
Sau96I GGNCC 2 cut(s) 302, 467
ScrFI CCNGG 2 cut(s) 101, 814
SduI GDGCHC 1 cut(s) 74
SetI ASST 7 cut(s) 34, 87, 457, 506, 557, 592, 779
SfcI CTRYAG 2 cut(s) 603, 868
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 2 cut(s) 302, 467
SmiMI CAYNNNNRTG 2 cut(s) 336, 705
SmlI CTYRAG 1 cut(s) 403
SmoI CTYRAG 1 cut(s) 403
Sse9I AATT 6 cut(s) 104, 137, 343, 714, 885, 898
SsiI CCGC 7 cut(s) 54, 79, 117, 248, 305, 437, 441
StyD4I CCNGG 2 cut(s) 99, 812
StyI CCWWGG 1 cut(s) 74
TaaI ACNGT 3 cut(s) 42, 415, 687
TaqI TCGA 4 cut(s) 35, 500, 565, 856
TasI AATT 6 cut(s) 104, 137, 343, 714, 885, 898
TauI GCSGC 3 cut(s) 57, 440, 443
TfiI GAWTC 3 cut(s) 221, 232, 488
Tru1I TTAA 3 cut(s) 363, 735, 901
Tru9I TTAA 3 cut(s) 363, 735, 901
TscAI CASTG 2 cut(s) 52, 790
TseFI GTSAC 2 cut(s) 153, 607
TseI GCWGC 4 cut(s) 57, 185, 194, 324
Tsp45I GTSAC 2 cut(s) 153, 607
TspDTI ATGAA 4 cut(s) 320, 354, 480, 845
TspRI CASTG 2 cut(s) 52, 790
VpaK11BI GGWCC 2 cut(s) 302, 467
XagI CCTNNNNNAGG 1 cut(s) 258
XapI RAATTY 3 cut(s) 137, 343, 714
Zsp2I ATGCAT 1 cut(s) 702
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.