MD15G1035800.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001271
Physical Location & Seq
Forward (+)
2509528 .. 2512003
2476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1035800.v1.1.491

Sequence Viewer

Length: 1596 bp
ATGTCTTCTTCTACTTTCTTCTGTGTTTCAGGTTCGAAACAGTGCAGTGGCTGCGGCTGCTGTGAGCAGAGCCCAAGGCGGACGCTCCGCCACAGCAACCCGGAATTGGGTTCTCGCGCCTCGGCCACTTTCCCTGCATATTCAATCCGAAAGTCACACTCTCTGGATTGGGGTGTTGGGCCTGTTGCTGCTCCACAAACCTTGCCCCTTCCAGAATCTTCGCTGATTCAGAGACCGGACGGGGTTCCTAAACAAGGGCAGGGTGAAAATCAAGTTGCCACAACCTCCATATCAGTACCGCTGGCTTCTTTCTCCAGCTGCATTCATCCAGATGAAAATGCTTGTGGGGGTTGTTTTAACTTTTGTCTCCCGACGAAAACTCAGGCAAATGATATCTCAAGCCATACTTTTAGCCTACAAAGATCAAACACTCTGGATGGTTTTTATAGTCCTCCAGTTAGCCCCCGAAGAACAAATGCGGCCGCAGATCCTTTACCTTCTTTTGGGGGTCCCCAAGATCACTATCAGGATTCATTCCCTTCGAGGTTATTACCAAAGGGACAATCTCGTAGTCTTGGTCATTCTCCTCTTCACAACCCCCCGTCTCGAAGTCAACGCTTCAACCCAAAAGGTTCAAATGGAACTGCAGTCACAGAGAGTTGTTCTGAGAGGGTTGAAAGTAGTAGTCGCGTCAGTGCTCACCCCTTGCCTCTGCCTCCTTCACCACAGTCATCTATCATGCATAACAATGCCGAAATTTTACCAGTATCATGTATTAAAGGACAATGGAAGAAGGGAAAACTTATTGGCAGGGGTACCTTAGGCAGTGTTTATCTCGCAACCAACCGAGAGACCGGGGCATTGTGCGCAATGAAGGAAGTTGATCTCATTCCTGACGATCCTAAATCTGCAGAATGTGTAAAGCAATTGGAGCAGGAAATTAAAATCCTCCGTACACTGAAGCATCCAAATATTGTGCAGTATTATGGTAGCGAAGTAATTGATAATCACTTCTGCATATATCTGGAGTATGTTTATATTCGGCCCGGAGCCATTGATAAATATGTCCAAGACCATATTGGAGCTATGACGGAATCTGTAGTTCGCAATTTCACTCGCCACATCCTCTCCGGGCTGGCCTTTCTGCATGGCACGAAGATAATTCACAGGGATGTAAGGGGTGCAAATTTGCGTCTTGATGCATCAGGAGTTGTTAAAATTGTCAATTTTGGGATTGCAAAACACCTCAATGGGCAATCAGATAATGTTTCTTTGAAGGGCAGTCCGAACTGGATGGCTCCTGAGGTCATAAGGACTGCAATGCAGAACAACGCGGATCCTGATTTACCTTTGGCCGTTGATATATGGAGCTTGGGTTGTACCATCATTGAGATGTTCACTGGAAAACCTCCTTGGAGTGAATGTACAGGACCTCAAGTTATGTTCAAGATGCTGAACAGAATCCCAGATATTCCCGAAACATTGTCTGCAGAAGGAAAGGACTTCCTCCATTGGTGCTTTCGGAGGGACCCTGCCAAGAGACCATCGGCTGCTGAGCTACTTGAACATCCATTTGTACATGCACAAAACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

532

Amino Acids

58.04

Weight (kDa)

8.5

Isoelectric Point (pI)

58.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 264 - 526 3.5e-62 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 266 - 522 2.9e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 868
Acc65I GGTACC 1 cut(s) 815
AccB1I GGYRCC 1 cut(s) 815
AccII CGCG 3 cut(s) 117, 690, 1334
AciI CCGC 7 cut(s) 54, 79, 88, 299, 479, 483, 1334
AclWI GGATC 4 cut(s) 482, 893, 1331, 1344
AcoI YGGCCR 3 cut(s) 123, 480, 1353
AcsI RAATTY 2 cut(s) 756, 1186
AcuI CTGAAG 1 cut(s) 980
AfaI GTAC 6 cut(s) 297, 817, 955, 1381, 1426, 1578
AfiI CCNNNNNNNGG 4 cut(s) 106, 107, 254, 503
AgsI TTSAA 7 cut(s) 144, 622, 636, 677, 1276, 1447, 1565
AjuI GAANNNNNNNTTGG 2 cut(s) 1396, 1428
AluBI AGCT 4 cut(s) 318, 1085, 1371, 1558
AluI AGCT 4 cut(s) 318, 1085, 1371, 1558
Alw21I GWGCWC 1 cut(s) 700
Alw26I GTCTC 5 cut(s) 226, 371, 609, 845, 1534
AlwI GGATC 4 cut(s) 482, 893, 1331, 1344
AlwNI CAGNNNCTG 1 cut(s) 51
AoxI GGCC 6 cut(s) 123, 179, 480, 1043, 1137, 1353
ApeKI GCWGC 5 cut(s) 51, 57, 188, 318, 1550
ApoI RAATTY 2 cut(s) 756, 1186
Asp718I GGTACC 1 cut(s) 815
AspLEI GCGC 2 cut(s) 119, 869
AspS9I GGNCC 5 cut(s) 179, 509, 1044, 1430, 1528
AsuC2I CCSGG 4 cut(s) 101, 856, 1047, 1132
AsuHPI GGTGA 3 cut(s) 275, 692, 714
AsuII TTCGAA 1 cut(s) 35
AvaII GGWCC 3 cut(s) 509, 1430, 1528
AxyI CCTNAGG 2 cut(s) 820, 1302
BamHI GGATCC 1 cut(s) 1336
BanI GGYRCC 1 cut(s) 815
BanII GRGCYC 1 cut(s) 74
Bbv12I GWGCWC 1 cut(s) 700
BbvI GCAGC 5 cut(s) 38, 44, 175, 305, 1537
BccI CCATC 4 cut(s) 431, 1288, 1391, 1552
BceAI ACGGC 1 cut(s) 1340
BcnI CCSGG 4 cut(s) 101, 856, 1047, 1132
BcoDI GTCTC 5 cut(s) 226, 371, 609, 845, 1534
BfmI CTRYAG 4 cut(s) 645, 909, 1098, 1488
BisI GCNGC 8 cut(s) 52, 55, 58, 189, 319, 480, 483, 1551
BlpI GCTNAGC 1 cut(s) 1554
BlsI GCNGC 8 cut(s) 53, 56, 59, 190, 320, 481, 484, 1552
Bme1390I CCNGG 4 cut(s) 101, 856, 1047, 1132
Bme18I GGWCC 3 cut(s) 509, 1430, 1528
BmgT120I GGNCC 5 cut(s) 179, 509, 1044, 1430, 1528
BmiI GGNNCC 9 cut(s) 246, 510, 511, 817, 1051, 1299, 1338, 1529, 1530
BmrFI CCNGG 4 cut(s) 101, 856, 1047, 1132
BmsI GCATC 4 cut(s) 973, 1189, 1211, 1440
BpmI CTGGAG 3 cut(s) 298, 438, 1046
Bpu1102I GCTNAGC 1 cut(s) 1554
Bpu14I TTCGAA 1 cut(s) 35
BpuEI CTTGAG 2 cut(s) 382, 1419
BpuMI CCSGG 4 cut(s) 101, 856, 1047, 1132
BsaBI GATNNNNATC 1 cut(s) 522
BsaI GGTCTC 3 cut(s) 226, 845, 1534
BsaJI CCNNGG 4 cut(s) 74, 120, 855, 1412
BsaWI WCCGGW 1 cut(s) 235
BsaXI ACNNNNNCTCC 6 cut(s) 1112, 1142, 1360, 1390, 1408, 1438
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 107, 254, 503
Bse1I ACTGG 4 cut(s) 455, 764, 1295, 1405
Bse21I CCTNAGG 2 cut(s) 820, 1302
Bse3DI GCAATG 2 cut(s) 876, 1326
Bse8I GATNNNNATC 1 cut(s) 522
BseDI CCNNGG 4 cut(s) 74, 120, 855, 1412
BseGI GGATG 7 cut(s) 325, 442, 964, 1122, 1177, 1299, 1567
BseJI GATNNNNATC 1 cut(s) 522
BseLI CCNNNNNNNGG 4 cut(s) 106, 107, 254, 503
BseMI GCAATG 2 cut(s) 876, 1326
BseMII CTCAG 4 cut(s) 395, 657, 1293, 1545
BseNI ACTGG 4 cut(s) 455, 764, 1295, 1405
BseRI GAGGAG 1 cut(s) 576
BseX3I CGGCCG 1 cut(s) 480
BseXI GCAGC 5 cut(s) 38, 44, 175, 305, 1537
BsgI GTGCAG 2 cut(s) 64, 998
Bsh1236I CGCG 3 cut(s) 117, 690, 1334
Bsh1285I CGRYCG 1 cut(s) 483
BshFI GGCC 6 cut(s) 125, 181, 482, 1045, 1139, 1355
BshNI GGYRCC 1 cut(s) 815
BsiEI CGRYCG 1 cut(s) 483
BsiHKAI GWGCWC 1 cut(s) 700
BsiSI CCGG 5 cut(s) 101, 236, 855, 1047, 1131
BslFI GGGAC 3 cut(s) 495, 573, 1541
BslI CCNNNNNNNGG 4 cut(s) 106, 107, 254, 503
BsmAI GTCTC 5 cut(s) 226, 371, 609, 845, 1534
BsmBI CGTCTC 1 cut(s) 609
BsmFI GGGAC 3 cut(s) 495, 573, 1541
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 6 cut(s) 125, 181, 482, 1045, 1139, 1355
Bso31I GGTCTC 3 cut(s) 226, 845, 1534
Bsp119I TTCGAA 1 cut(s) 35
Bsp1286I GDGCHC 2 cut(s) 74, 700
Bsp1407I TGTACA 2 cut(s) 1424, 1576
Bsp143I GATC 6 cut(s) 422, 487, 517, 883, 898, 1336
Bsp1720I GCTNAGC 1 cut(s) 1554
BspACI CCGC 7 cut(s) 54, 79, 88, 299, 479, 483, 1334
BspANI GGCC 6 cut(s) 125, 181, 482, 1045, 1139, 1355
BspCNI CTCAG 4 cut(s) 394, 658, 1294, 1546
BspFNI CGCG 3 cut(s) 117, 690, 1334
BspLI GGNNCC 9 cut(s) 246, 510, 511, 817, 1051, 1299, 1338, 1529, 1530
BspMAI CTGCAG 3 cut(s) 649, 913, 1492
BspPI GGATC 4 cut(s) 482, 893, 1331, 1344
BspT104I TTCGAA 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 815
BspTNI GGTCTC 3 cut(s) 226, 845, 1534
BsrDI GCAATG 2 cut(s) 876, 1326
BsrGI TGTACA 2 cut(s) 1424, 1576
BsrI ACTGG 4 cut(s) 455, 764, 1295, 1405
BssECI CCNNGG 4 cut(s) 74, 120, 855, 1412
BssMI GATC 6 cut(s) 422, 487, 517, 883, 898, 1336
BssT1I CCWWGG 2 cut(s) 74, 1412
Bst4CI ACNGT 2 cut(s) 42, 729
Bst6I CTCTTC 1 cut(s) 594
BstAPI GCANNNNNTGC 1 cut(s) 51
BstAUI TGTACA 2 cut(s) 1424, 1576
BstBI TTCGAA 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 303, 1137
BstDEI CTNAG 5 cut(s) 381, 666, 820, 1302, 1554
BstENI CCTNNNNNAGG 1 cut(s) 252
BstF5I GGATG 7 cut(s) 325, 442, 964, 1122, 1177, 1299, 1567
BstFNI CGCG 3 cut(s) 117, 690, 1334
BstHHI GCGC 2 cut(s) 119, 869
BstKTI GATC 6 cut(s) 425, 490, 520, 886, 901, 1339
BstMAI GTCTC 5 cut(s) 226, 371, 609, 845, 1534
BstMBI GATC 6 cut(s) 422, 487, 517, 883, 898, 1336
BstMCI CGRYCG 1 cut(s) 483
BstMWI GCNNNNNNNGC 4 cut(s) 51, 57, 866, 931
BstNSI RCATGY 1 cut(s) 1583
BstSCI CCNGG 4 cut(s) 99, 854, 1045, 1130
BstSFI CTRYAG 4 cut(s) 645, 909, 1098, 1488
BstUI CGCG 3 cut(s) 117, 690, 1334
BstV1I GCAGC 5 cut(s) 38, 44, 175, 305, 1537
BstX2I RGATCY 2 cut(s) 487, 1336
BstYI RGATCY 2 cut(s) 487, 1336
BstZI CGGCCG 1 cut(s) 480
Bsu36I CCTNAGG 2 cut(s) 820, 1302
BsuRI GGCC 6 cut(s) 125, 181, 482, 1045, 1139, 1355
BtsCI GGATG 7 cut(s) 325, 442, 964, 1122, 1177, 1299, 1567
BtsI GCAGTG 2 cut(s) 52, 832
BtsIMutI CAGTG 6 cut(s) 47, 52, 700, 832, 956, 1398
Cac8I GCNNGC 2 cut(s) 303, 1137
CaiI CAGNNNCTG 1 cut(s) 51
CciNI GCGGCCGC 1 cut(s) 480
CfoI GCGC 2 cut(s) 119, 869
Cfr13I GGNCC 5 cut(s) 179, 509, 1044, 1430, 1528
CseI GACGC 3 cut(s) 91, 679, 1181
Csp6I GTAC 6 cut(s) 296, 816, 954, 1380, 1425, 1577
CspCI CAANNNNNGTGG 2 cut(s) 183, 218
CviAII CATG 4 cut(s) 739, 771, 1148, 1580
CviQI GTAC 6 cut(s) 296, 816, 954, 1380, 1425, 1577
DdeI CTNAG 5 cut(s) 381, 666, 820, 1302, 1554
DpnI GATC 6 cut(s) 424, 489, 519, 885, 900, 1338
DpnII GATC 6 cut(s) 422, 487, 517, 883, 898, 1336
EaeI YGGCCR 3 cut(s) 123, 480, 1353
EagI CGGCCG 1 cut(s) 480
Eam1104I CTCTTC 1 cut(s) 594
EarI CTCTTC 1 cut(s) 594
EciI GGCGGA 2 cut(s) 77, 94
EclXI CGGCCG 1 cut(s) 480
Eco130I CCWWGG 2 cut(s) 74, 1412
Eco24I GRGCYC 1 cut(s) 74
Eco31I GGTCTC 3 cut(s) 226, 845, 1534
Eco32I GATATC 1 cut(s) 394
Eco47I GGWCC 3 cut(s) 509, 1430, 1528
Eco52I CGGCCG 1 cut(s) 480
Eco57I CTGAAG 1 cut(s) 980
Eco81I CCTNAGG 2 cut(s) 820, 1302
EcoNI CCTNNNNNAGG 1 cut(s) 252
EcoO109I RGGNCCY 3 cut(s) 509, 1430, 1528
EcoRV GATATC 1 cut(s) 394
EcoT14I CCWWGG 2 cut(s) 74, 1412
EcoT22I ATGCAT 2 cut(s) 744, 1204
EcoT38I GRGCYC 1 cut(s) 74
ErhI CCWWGG 2 cut(s) 74, 1412
Esp3I CGTCTC 1 cut(s) 609
FaeI CATG 4 cut(s) 742, 774, 1151, 1583
FalI AAGNNNNNCTT 2 cut(s) 391, 423
FaqI GGGAC 3 cut(s) 495, 573, 1541
FatI CATG 4 cut(s) 738, 770, 1147, 1579
Fnu4HI GCNGC 8 cut(s) 52, 55, 58, 189, 319, 480, 483, 1551
FokI GGATG 7 cut(s) 312, 449, 951, 1109, 1184, 1306, 1554
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 8 cut(s) 52, 55, 58, 189, 319, 480, 483, 1551
FspI TGCGCA 1 cut(s) 868
GlaI GCGC 2 cut(s) 118, 868
GluI GCNGC 8 cut(s) 52, 55, 58, 189, 319, 480, 483, 1551
GsuI CTGGAG 3 cut(s) 298, 438, 1046
HaeIII GGCC 6 cut(s) 125, 181, 482, 1045, 1139, 1355
HapII CCGG 5 cut(s) 101, 236, 855, 1047, 1131
HgaI GACGC 3 cut(s) 91, 679, 1181
HhaI GCGC 2 cut(s) 119, 869
Hin1II CATG 4 cut(s) 742, 774, 1151, 1583
Hin6I GCGC 2 cut(s) 117, 867
HinP1I GCGC 2 cut(s) 117, 867
HincII GTYRAC 1 cut(s) 614
HindII GTYRAC 1 cut(s) 614
HinfI GANTC 5 cut(s) 215, 226, 530, 1094, 1461
HpaII CCGG 5 cut(s) 101, 236, 855, 1047, 1131
HphI GGTGA 3 cut(s) 275, 692, 714
Hpy166II GTNNAC 3 cut(s) 614, 956, 1398
Hpy188I TCNGA 6 cut(s) 149, 231, 667, 1261, 1287, 1524
Hpy8I GTNNAC 3 cut(s) 614, 956, 1398
Hpy99I CGWCG 1 cut(s) 376
HpyAV CCTTC 8 cut(s) 218, 507, 549, 729, 787, 868, 1270, 1487
HpyCH4III ACNGT 2 cut(s) 42, 729
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 57, 866, 931
HpyF3I CTNAG 5 cut(s) 381, 666, 820, 1302, 1554
Hsp92II CATG 4 cut(s) 742, 774, 1151, 1583
HspAI GCGC 2 cut(s) 117, 867
KflI GGGWCCC 2 cut(s) 509, 1528
KpnI GGTACC 1 cut(s) 819
Kzo9I GATC 6 cut(s) 422, 487, 517, 883, 898, 1336
LmnI GCTCC 7 cut(s) 90, 196, 931, 1049, 1082, 1303, 1368
Lsp1109I GCAGC 5 cut(s) 38, 44, 175, 305, 1537
LweI GCATC 4 cut(s) 973, 1189, 1211, 1440
MaeIII GTNAC 2 cut(s) 153, 649
MalI GATC 6 cut(s) 424, 489, 519, 885, 900, 1338
MboI GATC 6 cut(s) 422, 487, 517, 883, 898, 1336
MboII GAAGA 6 cut(s) 10, 210, 480, 581, 802, 1168
MfeI CAATTG 1 cut(s) 926
MflI RGATCY 2 cut(s) 487, 1336
MhlI GDGCHC 2 cut(s) 74, 700
Mph1103I ATGCAT 2 cut(s) 744, 1204
MseI TTAA 4 cut(s) 357, 777, 942, 1215
MslI CAYNNNNRTG 5 cut(s) 330, 747, 1170, 1248, 1391
MspA1I CMGCKG 2 cut(s) 301, 318
MspI CCGG 5 cut(s) 101, 236, 855, 1047, 1131
MspR9I CCNGG 4 cut(s) 101, 856, 1047, 1132
MunI CAATTG 1 cut(s) 926
Mva1269I GAATGC 1 cut(s) 321
MvnI CGCG 3 cut(s) 117, 690, 1334
MwoI GCNNNNNNNGC 4 cut(s) 51, 57, 866, 931
NciI CCSGG 4 cut(s) 101, 856, 1047, 1132
NdeII GATC 6 cut(s) 422, 487, 517, 883, 898, 1336
NlaIII CATG 4 cut(s) 742, 774, 1151, 1583
NlaIV GGNNCC 9 cut(s) 246, 510, 511, 817, 1051, 1299, 1338, 1529, 1530
NmeAIII GCCGAG 1 cut(s) 101
NmuCI GTSAC 2 cut(s) 153, 649
NotI GCGGCCGC 1 cut(s) 480
NsbI TGCGCA 1 cut(s) 868
NsiI ATGCAT 2 cut(s) 744, 1204
NspI RCATGY 1 cut(s) 1583
NspV TTCGAA 1 cut(s) 35
PctI GAATGC 1 cut(s) 321
PfeI GAWTC 5 cut(s) 215, 226, 530, 1094, 1461
PkrI GCNGC 8 cut(s) 53, 56, 59, 190, 320, 481, 484, 1552
PpuMI RGGWCCY 3 cut(s) 509, 1430, 1528
Psp5II RGGWCCY 3 cut(s) 509, 1430, 1528
PspN4I GGNNCC 9 cut(s) 246, 510, 511, 817, 1051, 1299, 1338, 1529, 1530
PspPI GGNCC 5 cut(s) 179, 509, 1044, 1430, 1528
PspPPI RGGWCCY 3 cut(s) 509, 1430, 1528
PstI CTGCAG 3 cut(s) 649, 913, 1492
PstNI CAGNNNCTG 1 cut(s) 51
PsuI RGATCY 2 cut(s) 487, 1336
PvuII CAGCTG 1 cut(s) 318
RsaI GTAC 6 cut(s) 297, 817, 955, 1381, 1426, 1578
RsaNI GTAC 6 cut(s) 296, 816, 954, 1380, 1425, 1577
RseI CAYNNNNRTG 5 cut(s) 330, 747, 1170, 1248, 1391
SaqAI TTAA 4 cut(s) 357, 777, 942, 1215
SatI GCNGC 8 cut(s) 52, 55, 58, 189, 319, 480, 483, 1551
Sau3AI GATC 6 cut(s) 422, 487, 517, 883, 898, 1336
Sau96I GGNCC 5 cut(s) 179, 509, 1044, 1430, 1528
ScrFI CCNGG 4 cut(s) 101, 856, 1047, 1132
SduI GDGCHC 2 cut(s) 74, 700
SfaNI GCATC 4 cut(s) 973, 1189, 1211, 1440
SfcI CTRYAG 4 cut(s) 645, 909, 1098, 1488
SfuI TTCGAA 1 cut(s) 35
SinI GGWCC 3 cut(s) 509, 1430, 1528
SmiMI CAYNNNNRTG 5 cut(s) 330, 747, 1170, 1248, 1391
SmlI CTYRAG 2 cut(s) 397, 1434
SmoI CTYRAG 2 cut(s) 397, 1434
SsiI CCGC 7 cut(s) 54, 79, 88, 299, 479, 483, 1334
SspI AATATT 1 cut(s) 973
StyD4I CCNGG 4 cut(s) 99, 854, 1045, 1130
StyI CCWWGG 2 cut(s) 74, 1412
TaaI ACNGT 2 cut(s) 42, 729
TaqI TCGA 3 cut(s) 35, 542, 607
TatI WGTACW 2 cut(s) 1424, 1576
TauI GCSGC 3 cut(s) 57, 482, 485
TfiI GAWTC 5 cut(s) 215, 226, 530, 1094, 1461
Tru1I TTAA 4 cut(s) 357, 777, 942, 1215
Tru9I TTAA 4 cut(s) 357, 777, 942, 1215
TscAI CASTG 6 cut(s) 47, 52, 700, 832, 963, 1405
TseFI GTSAC 2 cut(s) 153, 649
TseI GCWGC 5 cut(s) 51, 57, 188, 318, 1550
Tsp45I GTSAC 2 cut(s) 153, 649
TspDTI ATGAA 4 cut(s) 314, 348, 522, 887
TspGWI ACGGA 2 cut(s) 941, 1106
TspRI CASTG 6 cut(s) 47, 52, 700, 832, 963, 1405
VpaK11BI GGWCC 3 cut(s) 509, 1430, 1528
XagI CCTNNNNNAGG 1 cut(s) 252
XapI RAATTY 2 cut(s) 756, 1186
XceI RCATGY 1 cut(s) 1583
XcmI CCANNNNNNNNNTGG 1 cut(s) 1076
Zsp2I ATGCAT 2 cut(s) 744, 1204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.