Rmu_sc0001059.1_g000001

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001059.1
OG0001271
Physical Location & Seq
Forward (+)
1 .. 2588
2588 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001059.1_g000001.1.cds

Sequence Viewer

Length: 1174 bp
aagttcccctataaacagtcccacggctagaagtccccttcgacccccacaagcatcatccccattgcatccccggttatccgttacctctgaagtccatccattgcctctgcctcctggagcaagcctcccttcacctagtgtaccatcaccacaacctgcactcacacctaagccagagtcccagccgattaaagggcaatggcaaaagggaaagcttattggtcgtggcacctttggcagtgtttatgttgctaccaatagagaaactggagctttatgtgcaatgaaggaagttgaactacttcctgatgacccgaaatccgcagaatgtataaagcagctacagcaggaaatcaaagtcctcagccagctcaagcatcctaatattgtgcagtactatggaagtgaaattgttgaagatcggttttttatatatctggaatatgttcatcctggttctattaacaaatatgttcatgaacactgtggagccatgacagaagctgttgttcgcagttttactcgccatatagtctctgggttggcttacttgcacagcacaaaaacaatacacagggacatcaaaggggctaatttgcttgtggattcatgtggggttgtcaagctagctgactttggaatggccaagcatctttcaggacacgtagggaatctttccctaaagggaagtccatactggatggctccagagctgatgcaatctgagatgcataaagataacagctctgatttggctcttgctgttgatatatggagtttgggttgtactattattgaaatgttcacaggaaaacctccatggagtgagtatgaaggggctgcagctatgtttaaagttatgaaggatactccaccttttccagaaactttgtcacctgagggtaaggacttccttaggtgctgcttccaaagaaatcctgctgagaggtcaactgctgcaatgctattagaacatcgatttataaaaaactcccagctgcaggaagtcccatctacgacctataatgcctctaacgggatgaatttcttgtctgtgttatgtcgacaggaaagaccccagactccaattgaacttgaaagaagacttgatagttcactgatgattcccagcattggcatgcaattaactacgggaaaggggcctctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

390

Amino Acids

42.99

Weight (kDa)

8.06

Isoelectric Point (pI)

53.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 987
Acc36I ACCTGC 1 cut(s) 167
AccB1I GGYRCC 1 cut(s) 231
AccI GTMKAC 1 cut(s) 1067
AciI CCGC 1 cut(s) 325
AcoI YGGCCR 1 cut(s) 646
AcsI RAATTY 1 cut(s) 1046
AcuI CTGAAG 1 cut(s) 112
AdeI CACNNNGTG 1 cut(s) 141
AfaI GTAC 3 cut(s) 145, 399, 791
AfiI CCNNNNNNNGG 4 cut(s) 195, 1004, 1039, 1137
AflIII ACRYGT 1 cut(s) 665
AgsI TTSAA 5 cut(s) 300, 420, 801, 1095, 1101
AjnI CCWGG 2 cut(s) 116, 455
Alw26I GTCTC 1 cut(s) 542
AlwNI CAGNNNCTG 1 cut(s) 507
AoxI GGCC 2 cut(s) 646, 1164
ApeKI GCWGC 6 cut(s) 341, 843, 846, 925, 960, 1001
ApoI RAATTY 1 cut(s) 1046
Asp700I GAANNNNTTC 2 cut(s) 304, 448
AspS9I GGNCC 1 cut(s) 1164
AsuC2I CCSGG 1 cut(s) 74
AsuHPI GGTGA 3 cut(s) 127, 142, 889
AsuNHI GCTAGC 1 cut(s) 629
AxyI CCTNAGG 2 cut(s) 901, 918
BalI TGGCCA 1 cut(s) 648
BanI GGYRCC 1 cut(s) 231
BarI GAAGNNNNNNTAC 2 cut(s) 286, 318
BbsI GAAGAC 1 cut(s) 1112
BbvCI CCTCAGC 1 cut(s) 366
BbvI GCAGC 6 cut(s) 353, 830, 858, 912, 947, 988
BccI CCATC 4 cut(s) 106, 155, 698, 1022
BceAI ACGGC 1 cut(s) 40
BciT130I CCWGG 2 cut(s) 118, 457
BciVI GTATCC 1 cut(s) 863
BcnI CCSGG 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 542
BfaI CTAG 3 cut(s) 28, 139, 630
BfmI CTRYAG 3 cut(s) 345, 844, 1002
BfuAI ACCTGC 1 cut(s) 167
BfuI GTATCC 1 cut(s) 863
BisI GCNGC 6 cut(s) 342, 844, 847, 926, 961, 1002
BlsI GCNGC 6 cut(s) 343, 845, 848, 927, 962, 1003
BmcAI AGTACT 1 cut(s) 399
Bme1390I CCNGG 3 cut(s) 74, 118, 457
BmgT120I GGNCC 1 cut(s) 1164
BmiI GGNNCC 4 cut(s) 233, 494, 709, 1165
BmrFI CCNGG 3 cut(s) 74, 118, 457
BmsI GCATC 6 cut(s) 63, 77, 389, 662, 709, 721
BmtI GCTAGC 1 cut(s) 633
BpiI GAAGAC 1 cut(s) 1112
BplI GAGNNNNNCTC 2 cut(s) 112, 144
BpmI CTGGAG 3 cut(s) 139, 292, 694
Bpu10I CCTNAGC 2 cut(s) 172, 366
BpuEI CTTGAG 1 cut(s) 360
BpuMI CCSGG 1 cut(s) 74
Bsa29I ATCGAT 1 cut(s) 980
BsaAI YACGTR 1 cut(s) 668
BsaJI CCNNGG 3 cut(s) 22, 72, 822
BsaXI ACNNNNNCTCC 2 cut(s) 770, 800
Bsc4I CCNNNNNNNGG 4 cut(s) 195, 1004, 1039, 1137
Bse1I ACTGG 2 cut(s) 275, 705
Bse21I CCTNAGG 2 cut(s) 901, 918
Bse3DI GCAATG 5 cut(s) 63, 102, 207, 292, 970
BseBI CCWGG 2 cut(s) 118, 457
BseCI ATCGAT 1 cut(s) 980
BseDI CCNNGG 3 cut(s) 22, 72, 822
BseGI GGATG 7 cut(s) 57, 68, 98, 380, 452, 709, 1048
BseLI CCNNNNNNNGG 4 cut(s) 195, 1004, 1039, 1137
BseMI GCAATG 5 cut(s) 63, 102, 207, 292, 970
BseMII CTCAG 4 cut(s) 380, 718, 892, 937
BseNI ACTGG 2 cut(s) 275, 705
BseXI GCAGC 6 cut(s) 353, 830, 858, 912, 947, 988
BseYI CCCAGC 3 cut(s) 184, 997, 1131
BsgI GTGCAG 2 cut(s) 145, 414
BshFI GGCC 2 cut(s) 648, 1166
BshNI GGYRCC 1 cut(s) 231
BshVI ATCGAT 1 cut(s) 980
BsiSI CCGG 1 cut(s) 74
BslFI GGGAC 5 cut(s) 4, 19, 167, 594, 996
BslI CCNNNNNNNGG 4 cut(s) 195, 1004, 1039, 1137
BsmAI GTCTC 1 cut(s) 542
BsmFI GGGAC 5 cut(s) 4, 19, 167, 594, 996
BsnI GGCC 2 cut(s) 648, 1166
Bsp143I GATC 1 cut(s) 422
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 1 cut(s) 325
BspANI GGCC 2 cut(s) 648, 1166
BspCNI CTCAG 4 cut(s) 379, 719, 893, 938
BspDI ATCGAT 1 cut(s) 980
BspHI TCATGA 1 cut(s) 479
BspLI GGNNCC 4 cut(s) 233, 494, 709, 1165
BspMAI CTGCAG 2 cut(s) 848, 1006
BspMI ACCTGC 1 cut(s) 167
BspOI GCTAGC 1 cut(s) 633
BspT107I GGYRCC 1 cut(s) 231
BsrDI GCAATG 5 cut(s) 63, 102, 207, 292, 970
BsrI ACTGG 2 cut(s) 275, 705
BssECI CCNNGG 3 cut(s) 22, 72, 822
BssMI GATC 1 cut(s) 422
BssT1I CCWWGG 1 cut(s) 822
Bst2UI CCWGG 2 cut(s) 118, 457
Bst4CI ACNGT 2 cut(s) 18, 489
BstBAI YACGTR 1 cut(s) 668
BstC8I GCNNGC 4 cut(s) 125, 372, 631, 1143
BstDEI CTNAG 6 cut(s) 172, 366, 727, 901, 918, 946
BstDSI CCRYGG 2 cut(s) 22, 822
BstF5I GGATG 7 cut(s) 57, 68, 98, 380, 452, 709, 1048
BstKTI GATC 1 cut(s) 425
BstMAI GTCTC 1 cut(s) 542
BstMBI GATC 1 cut(s) 422
BstMWI GCNNNNNNNGC 3 cut(s) 238, 282, 347
BstNI CCWGG 2 cut(s) 118, 457
BstNSI RCATGY 1 cut(s) 1145
BstSCI CCNGG 3 cut(s) 72, 116, 455
BstSFI CTRYAG 3 cut(s) 345, 844, 1002
BstV1I GCAGC 6 cut(s) 353, 830, 858, 912, 947, 988
BstV2I GAAGAC 1 cut(s) 1112
Bsu15I ATCGAT 1 cut(s) 980
Bsu36I CCTNAGG 2 cut(s) 901, 918
BsuI GTATCC 1 cut(s) 863
BsuRI GGCC 2 cut(s) 648, 1166
BsuTUI ATCGAT 1 cut(s) 980
BtgI CCRYGG 2 cut(s) 22, 822
BtsCI GGATG 7 cut(s) 57, 68, 98, 380, 452, 709, 1048
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 3 cut(s) 248, 485, 1119
BveI ACCTGC 1 cut(s) 167
Cac8I GCNNGC 4 cut(s) 125, 372, 631, 1143
CaiI CAGNNNCTG 1 cut(s) 507
CciI TCATGA 1 cut(s) 479
Cfr13I GGNCC 1 cut(s) 1164
ClaI ATCGAT 1 cut(s) 980
Csp6I GTAC 3 cut(s) 144, 398, 790
CviAII CATG 5 cut(s) 480, 497, 613, 823, 1142
CviQI GTAC 3 cut(s) 144, 398, 790
DdeI CTNAG 6 cut(s) 172, 366, 727, 901, 918, 946
DpnI GATC 1 cut(s) 424
DpnII GATC 1 cut(s) 422
DraI TTTAAA 1 cut(s) 857
DraIII CACNNNGTG 1 cut(s) 141
EaeI YGGCCR 1 cut(s) 646
Eco130I CCWWGG 1 cut(s) 822
Eco57I CTGAAG 1 cut(s) 112
Eco81I CCTNAGG 2 cut(s) 901, 918
EcoO109I RGGNCCY 1 cut(s) 1164
EcoRII CCWGG 2 cut(s) 116, 455
EcoT14I CCWWGG 1 cut(s) 822
EcoT22I ATGCAT 1 cut(s) 736
ErhI CCWWGG 1 cut(s) 822
FaeI CATG 5 cut(s) 483, 500, 616, 826, 1145
FalI AAGNNNNNCTT 2 cut(s) 116, 148
FaqI GGGAC 5 cut(s) 4, 19, 167, 594, 996
FatI CATG 5 cut(s) 479, 496, 612, 822, 1141
FblI GTMKAC 1 cut(s) 1067
Fnu4HI GCNGC 6 cut(s) 342, 844, 847, 926, 961, 1002
FokI GGATG 7 cut(s) 44, 55, 85, 367, 439, 716, 1055
Fsp4HI GCNGC 6 cut(s) 342, 844, 847, 926, 961, 1002
FspBI CTAG 3 cut(s) 28, 139, 630
GluI GCNGC 6 cut(s) 342, 844, 847, 926, 961, 1002
GsaI CCCAGC 3 cut(s) 188, 1001, 1135
GsuI CTGGAG 3 cut(s) 139, 292, 694
HaeIII GGCC 2 cut(s) 648, 1166
HapII CCGG 1 cut(s) 74
Hin1II CATG 5 cut(s) 483, 500, 616, 826, 1145
HincII GTYRAC 2 cut(s) 955, 1068
HindII GTYRAC 2 cut(s) 955, 1068
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 5 cut(s) 180, 609, 674, 1085, 1127
HpaII CCGG 1 cut(s) 74
HphI GGTGA 3 cut(s) 127, 142, 889
Hpy166II GTNNAC 5 cut(s) 144, 808, 955, 1068, 1119
Hpy188I TCNGA 4 cut(s) 92, 728, 752, 1173
Hpy188III TCNNGA 6 cut(s) 309, 441, 480, 661, 711, 885
Hpy8I GTNNAC 5 cut(s) 144, 808, 955, 1068, 1119
HpyAV CCTTC 5 cut(s) 48, 142, 284, 831, 860
HpyCH4III ACNGT 2 cut(s) 18, 489
HpyCH4IV ACGT 1 cut(s) 667
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 282, 347
HpyF3I CTNAG 6 cut(s) 172, 366, 727, 901, 918, 946
HpySE526I ACGT 1 cut(s) 667
Hsp92II CATG 5 cut(s) 483, 500, 616, 826, 1145
Kzo9I GATC 1 cut(s) 422
LmnI GCTCC 4 cut(s) 120, 273, 492, 713
Lsp1109I GCAGC 6 cut(s) 353, 830, 858, 912, 947, 988
LweI GCATC 6 cut(s) 63, 77, 389, 662, 709, 721
MaeI CTAG 3 cut(s) 28, 139, 630
MaeII ACGT 1 cut(s) 667
MaeIII GTNAC 2 cut(s) 83, 895
MalI GATC 1 cut(s) 424
MboI GATC 1 cut(s) 422
MboII GAAGA 2 cut(s) 432, 1117
MfeI CAATTG 1 cut(s) 1090
MlsI TGGCCA 1 cut(s) 648
MluCI AATT 5 cut(s) 412, 596, 1046, 1090, 1146
MluNI TGGCCA 1 cut(s) 648
MlyI GAGTC 2 cut(s) 189, 1079
MnlI CCTC 9 cut(s) 98, 118, 124, 138, 375, 829, 896, 943, 1043
Mox20I TGGCCA 1 cut(s) 648
Mph1103I ATGCAT 1 cut(s) 736
MroXI GAANNNNTTC 2 cut(s) 304, 448
MscI TGGCCA 1 cut(s) 648
MseI TTAA 4 cut(s) 193, 466, 856, 1149
MslI CAYNNNNRTG 1 cut(s) 1140
Msp20I TGGCCA 1 cut(s) 648
MspA1I CMGCKG 1 cut(s) 1001
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 3 cut(s) 74, 118, 457
MunI CAATTG 1 cut(s) 1090
MvaI CCWGG 2 cut(s) 118, 457
MwoI GCNNNNNNNGC 3 cut(s) 238, 282, 347
NciI CCSGG 1 cut(s) 74
NcoI CCATGG 1 cut(s) 822
NdeII GATC 1 cut(s) 422
NheI GCTAGC 1 cut(s) 629
NlaIII CATG 5 cut(s) 483, 500, 616, 826, 1145
NlaIV GGNNCC 4 cut(s) 233, 494, 709, 1165
NmuCI GTSAC 1 cut(s) 895
NsiI ATGCAT 1 cut(s) 736
NspI RCATGY 1 cut(s) 1145
PaeI GCATGC 1 cut(s) 1145
PagI TCATGA 1 cut(s) 479
PdmI GAANNNNTTC 2 cut(s) 304, 448
PfeI GAWTC 3 cut(s) 609, 674, 1127
PfoI TCCNGGA 1 cut(s) 116
PkrI GCNGC 6 cut(s) 343, 845, 848, 927, 962, 1003
PleI GAGTC 2 cut(s) 188, 1079
PpsI GAGTC 2 cut(s) 188, 1079
Ppu21I YACGTR 1 cut(s) 668
PsiI TTATAA 1 cut(s) 987
Psp6I CCWGG 2 cut(s) 116, 455
PspFI CCCAGC 3 cut(s) 184, 997, 1131
PspGI CCWGG 2 cut(s) 116, 455
PspN4I GGNNCC 4 cut(s) 233, 494, 709, 1165
PspPI GGNCC 1 cut(s) 1164
PstI CTGCAG 2 cut(s) 848, 1006
PstNI CAGNNNCTG 1 cut(s) 507
PvuII CAGCTG 1 cut(s) 1001
RsaI GTAC 3 cut(s) 145, 399, 791
RsaNI GTAC 3 cut(s) 144, 398, 790
RseI CAYNNNNRTG 1 cut(s) 1140
SalI GTCGAC 1 cut(s) 1066
SaqAI TTAA 4 cut(s) 193, 466, 856, 1149
SatI GCNGC 6 cut(s) 342, 844, 847, 926, 961, 1002
Sau3AI GATC 1 cut(s) 422
Sau96I GGNCC 1 cut(s) 1164
ScaI AGTACT 1 cut(s) 399
SchI GAGTC 2 cut(s) 189, 1079
ScrFI CCNGG 3 cut(s) 74, 118, 457
SfaNI GCATC 6 cut(s) 63, 77, 389, 662, 709, 721
SfcI CTRYAG 3 cut(s) 345, 844, 1002
SmiMI CAYNNNNRTG 1 cut(s) 1140
SmlI CTYRAG 1 cut(s) 375
SmoI CTYRAG 1 cut(s) 375
SphI GCATGC 1 cut(s) 1145
Sse9I AATT 5 cut(s) 412, 596, 1046, 1090, 1146
SsiI CCGC 1 cut(s) 325
SspI AATATT 1 cut(s) 389
SspMI CTAG 3 cut(s) 28, 139, 630
StyD4I CCNGG 3 cut(s) 72, 116, 455
StyI CCWWGG 1 cut(s) 822
TaaI ACNGT 2 cut(s) 18, 489
TaiI ACGT 1 cut(s) 670
TaqI TCGA 3 cut(s) 41, 980, 1067
TasI AATT 5 cut(s) 412, 596, 1046, 1090, 1146
TatI WGTACW 2 cut(s) 397, 789
TfiI GAWTC 3 cut(s) 609, 674, 1127
Tru1I TTAA 4 cut(s) 193, 466, 856, 1149
Tru9I TTAA 4 cut(s) 193, 466, 856, 1149
TscAI CASTG 3 cut(s) 248, 492, 1126
TseFI GTSAC 1 cut(s) 895
TseI GCWGC 6 cut(s) 341, 843, 846, 925, 960, 1001
Tsp45I GTSAC 1 cut(s) 895
TspDTI ATGAA 8 cut(s) 303, 441, 468, 496, 601, 850, 879, 1059
TspGWI ACGGA 1 cut(s) 71
TspRI CASTG 3 cut(s) 248, 492, 1126
XapI RAATTY 1 cut(s) 1046
XceI RCATGY 1 cut(s) 1145
XcmI CCANNNNNNNNNTGG 1 cut(s) 537
XmiI GTMKAC 1 cut(s) 1067
XmnI GAANNNNTTC 2 cut(s) 304, 448
XspI CTAG 3 cut(s) 28, 139, 630
ZrmI AGTACT 1 cut(s) 399
Zsp2I ATGCAT 1 cut(s) 736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.