Rw6G035340

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001271
Physical Location & Seq
Reverse (-)
59883774 .. 59888136
4363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G035340.1

Sequence Viewer

Length: 2214 bp
ATGACTCATTTTGATATATTTGAAGGGGGCATTCTGACATTTTCAACCGACGGCTTCAACAAAACCCACAGCAGCACCACCAGCTGCCACCGCCTACCACTTCCACAACCTTCTTCTTCAACCTTTCCCAATCACCTAATGCCTTCTTCTTCTTCTGCTCCATCTCAATCTCTTCAAAAACGAACCTTTTTTACATCTTCATCTTCTACTCCTGCTTCCTCCTCCTCCTCTGTCTCAGCTCTGAGACCCGGGATCAACAGAAGCGGCAGTGATGTTAACAGCCCACGGCGGCTCACGCGCCAGAGGAGGCTGCGCTACCTCACGAGCCCCGAGGATCTCGACTCTTACGGGGTCCCGGAGAAGTCTCGCTCATCGCCCACCTCGCCTGAATATTCGACCCGTAGATCACGCTCTCCGTGTGGCGAGTCAAACCCAAATTCACGCTCTCCTCCCGGTGGCGAGTCGGCCCCATGTTCTCGGTCTTCGGTCGGTGACTGGGCCCGAAAGTCATGCTCCTCTTCTTTCGTTGCTGGGCCTCAAGGCCCACAACCCTTGCCGCTTCCGGAGAACAACCGGAGACCCGACTGCGTCTCCTTCTTCAGCCCTGTGGTCGGCAGGAAGAGCGTCGATCAAGCTGCCACAAGCTCATCATCACCGGCGAAGTCTTCCAAGAACCGATGCCGAGATGCAAACAATTCCTGCGGGGGTGGGGGCGGGAGTGGGGGTTTTAATTTCCATTTGGGTTTGAATGTTCAATCTAGGACTACTAGGAGTGCCCCACAGACTGTTTTTAGCTCAAGTCATCATCCCGTTAGCCCACAAAGATCAAACACAGTGGATATTTTGCCTTGTCAGTCACATGTTGCAGGCAACTATTATAACAGGGGAATGGGAATGGCAGTGTCACCGGAGGCTACCTTTGAACGTGAAACTTACAACTTGAGCAGGAGTGCTCCAACCAGTGTTCTCTCAAGTCCTGCGGTTAGCCCGCGCCGATCAACCACTGTGGATCCTTTTCCTTTTTTCACGACCCATCAAGATCAATATCAGGATGGTGCTACCTTTCCCTCCTTTTTGTCACCAAACAAGCCTACACTCAGTCCCACTCATTCTCCACTCCATAGCCCTACGCCTCGAAGTCCACGCTTCAGCCCCAATGCTTCAAACTATGAGAGTAGTCTTGCCAATGCTCACCCGTTGCCTCTGCCTCCTGGAGCCGCAGTGCCAGCACAGCCGCCCTCTATCACTATGCATCAAAATAATGTAGTAACCGTATCATCAATGAAGGGTCAATGGCAGAAGGGGAAACTTATCGGACGGGGTACCTTTGGTAGTGTTTATCTTGCAACCAACCGAGAAACTGGGGCCTTGTGTGCAATGAAGGAAGTTGATCTCATTCCTGATGATCCTAAATCTGCAGAAAGTATAAAGCAATTGGAGCAGGAAATTAAAGTCCTCCGTACGCTAAAGCATCCAAATATTGTGCAGTACTATGGTAGTGAAGTGATTGATGATCATTTCTACATATATTTAGAATATGTTCACCCCGGATCAATAAATAAATATGTCCGAGACCATATTGGAGCTATGACTGAATCCGTAATCCGCAATTTTACCCGCCATATCCTCTGTGGGTTGGCTTTTTTGCATGACACGAAGACAATTCACAGGGATATTAAAGGTGCAAATTTGCTCGTTGATGCATCAGGCGTTGTCAAACTTGCCGATTTTGGGATGGCAAAACATCTTGGTGCGCATTCATACAATCTTTCTTTGAAGGGCAGCCCATACTGGATGGCTCCTGAGGTCATAAAGGCTGTGATACAGAATGATGGTGATCCTAATCTTGCTTTGGCTGTTGATATATGGAGCTTGGGTTGTACCATCATCGAGATGTTCAATGGAAAACCGCCTTGGAGTGAATTTGAAGGGCCACAAGCTATGTTCAAGGTTCTGCACAGAACCCCGGATATACCAGAAAAATTATCTGCAGAAGGAAAGGAATTCCTCAGTTTGTGCTTCAAAAGGAACCCCGCAGAGAGGCCATCAGCTAAGAAGCTACTTGATCATCCTTTTGTACGAATTTCAAATGATCAAAATGTCTCATCTTATACTCGGGCTTTTTCTCTGGTAATGGATAAATTGAATAGCCCGACGGACCATATTAAGCAAAAACTTGATCGAATGCTGGTCTGTCCACGCACTTCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

737

Amino Acids

80.46

Weight (kDa)

9.37

Isoelectric Point (pI)

66.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 432 - 693 3.7e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 435 - 688 6.4e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 879
Acc16I TGCGCA 1 cut(s) 1755
Acc65I GGTACC 1 cut(s) 1322
AccB1I GGYRCC 1 cut(s) 1322
AccII CGCG 2 cut(s) 298, 991
AccIII TCCGGA 1 cut(s) 562
AclWI GGATC 7 cut(s) 260, 342, 1004, 1017, 1400, 1558, 1832
AcsI RAATTY 5 cut(s) 436, 1687, 1922, 2003, 2082
AcuI CTGAAG 2 cut(s) 583, 1132
AfaI GTAC 5 cut(s) 1324, 1462, 1490, 1882, 2079
AfiI CCNNNNNNNGG 4 cut(s) 455, 611, 1731, 2040
AflIII ACRYGT 1 cut(s) 859
AjnI CCWGG 1 cut(s) 1210
AjuI GAANNNNNNNTTGG 2 cut(s) 1897, 1929
Alw21I GWGCWC 1 cut(s) 955
Alw26I GTCTC 7 cut(s) 238, 238, 369, 571, 595, 1566, 2107
AlwI GGATC 7 cut(s) 260, 342, 1004, 1017, 1400, 1558, 1832
Ama87I CYCGRG 3 cut(s) 248, 329, 2115
Aor13HI TCCGGA 1 cut(s) 562
AoxI GGCC 7 cut(s) 465, 498, 533, 541, 1365, 1931, 2042
ApaI GGGCCC 1 cut(s) 502
ApeKI GCWGC 5 cut(s) 72, 84, 310, 635, 1782
ApoI RAATTY 5 cut(s) 436, 1687, 1922, 2003, 2082
Asp700I GAANNNNTTC 1 cut(s) 1539
Asp718I GGTACC 1 cut(s) 1322
AspLEI GCGC 4 cut(s) 300, 315, 993, 1756
AspS9I GGNCC 9 cut(s) 352, 466, 498, 499, 533, 542, 1365, 1931, 2158
AsuC2I CCSGG 6 cut(s) 249, 250, 356, 453, 1548, 1967
AsuHPI GGTGA 8 cut(s) 125, 503, 645, 897, 1071, 1184, 1535, 1847
AvaI CYCGRG 3 cut(s) 248, 329, 2115
AvaII GGWCC 2 cut(s) 352, 2158
AxyI CCTNAGG 1 cut(s) 1803
BaeGI GKGCMC 2 cut(s) 502, 778
BamHI GGATCC 1 cut(s) 1009
BanI GGYRCC 1 cut(s) 1322
BanII GRGCYC 2 cut(s) 329, 502
BauI CACGAG 1 cut(s) 322
BbsI GAAGAC 3 cut(s) 474, 657, 1664
Bbv12I GWGCWC 1 cut(s) 955
BbvI GCAGC 5 cut(s) 71, 84, 297, 622, 1794
BccI CCATC 8 cut(s) 169, 1041, 1046, 1729, 1789, 1826, 1892, 2053
BceAI ACGGC 2 cut(s) 67, 302
BcgI CGANNNNNNTGC 4 cut(s) 492, 526, 617, 651
BciT130I CCWGG 1 cut(s) 1212
BclI TGATCA 3 cut(s) 1513, 2065, 2092
BcnI CCSGG 6 cut(s) 249, 250, 356, 453, 1548, 1967
BcoDI GTCTC 7 cut(s) 238, 238, 369, 571, 595, 1566, 2107
BfaI CTAG 2 cut(s) 759, 768
BfmI CTRYAG 2 cut(s) 1416, 1989
BmcAI AGTACT 1 cut(s) 1490
Bme1390I CCNGG 7 cut(s) 249, 250, 356, 453, 1212, 1548, 1967
Bme18I GGWCC 2 cut(s) 352, 2158
BmeT110I CYCGRG 3 cut(s) 248, 329, 2115
BmgT120I GGNCC 9 cut(s) 352, 466, 498, 499, 533, 542, 1365, 1931, 2158
BmrFI CCNGG 7 cut(s) 249, 250, 356, 453, 1212, 1548, 1967
BmrI ACTGGG 2 cut(s) 505, 1371
BmsI GCATC 6 cut(s) 668, 676, 1261, 1480, 1690, 1712
BmuI ACTGGG 2 cut(s) 505, 1371
BpiI GAAGAC 3 cut(s) 474, 657, 1664
BpmI CTGGAG 1 cut(s) 1233
BpuEI CTTGAG 4 cut(s) 522, 781, 955, 961
BpuMI CCSGG 6 cut(s) 249, 250, 356, 453, 1548, 1967
BsaBI GATNNNNATC 3 cut(s) 1044, 1836, 1842
BsaI GGTCTC 3 cut(s) 238, 571, 1566
BsaJI CCNNGG 6 cut(s) 248, 284, 330, 1546, 1913, 1965
BsaWI WCCGGW 3 cut(s) 562, 573, 907
Bsc4I CCNNNNNNNGG 4 cut(s) 455, 611, 1731, 2040
Bse118I RCCGGY 1 cut(s) 655
Bse1I ACTGG 4 cut(s) 500, 960, 1366, 1796
Bse21I CCTNAGG 1 cut(s) 1803
Bse3DI GCAATG 1 cut(s) 1383
Bse8I GATNNNNATC 3 cut(s) 1044, 1836, 1842
BseAI TCCGGA 1 cut(s) 562
BseBI CCWGG 1 cut(s) 1212
BseDI CCNNGG 6 cut(s) 248, 284, 330, 1546, 1913, 1965
BseGI GGATG 6 cut(s) 805, 1057, 1471, 1740, 1800, 2068
BseJI GATNNNNATC 3 cut(s) 1044, 1836, 1842
BseLI CCNNNNNNNGG 4 cut(s) 455, 611, 1731, 2040
BseMI GCAATG 1 cut(s) 1383
BseMII CTCAG 5 cut(s) 233, 249, 1111, 1794, 2023
BseNI ACTGG 4 cut(s) 500, 960, 1366, 1796
BseRI GAGGAG 6 cut(s) 211, 214, 217, 319, 438, 505
BseSI GKGCMC 2 cut(s) 502, 778
BseXI GCAGC 5 cut(s) 71, 84, 297, 622, 1794
BseYI CCCAGC 1 cut(s) 530
BsgI GTGCAG 2 cut(s) 1505, 1940
Bsh1236I CGCG 2 cut(s) 298, 991
Bsh1285I CGRYCG 1 cut(s) 489
BshFI GGCC 7 cut(s) 467, 500, 535, 543, 1367, 1933, 2044
BshNI GGYRCC 1 cut(s) 1322
BsiEI CGRYCG 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 955
BsiHKCI CYCGRG 3 cut(s) 248, 329, 2115
BsiSI CCGG 9 cut(s) 249, 356, 453, 563, 574, 656, 908, 1548, 1967
BsiWI CGTACG 1 cut(s) 1460
BslFI GGGAC 2 cut(s) 338, 1086
BslI CCNNNNNNNGG 4 cut(s) 455, 611, 1731, 2040
BsmAI GTCTC 7 cut(s) 238, 238, 369, 571, 595, 1566, 2107
BsmBI CGTCTC 1 cut(s) 595
BsmFI GGGAC 2 cut(s) 338, 1086
BsmI GAATGC 3 cut(s) 30, 1756, 2190
BsnI GGCC 7 cut(s) 467, 500, 535, 543, 1367, 1933, 2044
Bso31I GGTCTC 3 cut(s) 238, 571, 1566
BsoBI CYCGRG 3 cut(s) 248, 329, 2115
Bsp120I GGGCCC 1 cut(s) 498
Bsp1286I GDGCHC 4 cut(s) 329, 502, 778, 955
Bsp13I TCCGGA 1 cut(s) 562
BspANI GGCC 7 cut(s) 467, 500, 535, 543, 1367, 1933, 2044
BspCNI CTCAG 5 cut(s) 234, 248, 1110, 1795, 2022
BspEI TCCGGA 1 cut(s) 562
BspFNI CGCG 2 cut(s) 298, 991
BspMAI CTGCAG 2 cut(s) 1420, 1993
BspPI GGATC 7 cut(s) 260, 342, 1004, 1017, 1400, 1558, 1832
BspQI GCTCTTC 1 cut(s) 614
BspT107I GGYRCC 1 cut(s) 1322
BspTNI GGTCTC 3 cut(s) 238, 571, 1566
BsrDI GCAATG 1 cut(s) 1383
BsrFI RCCGGY 1 cut(s) 655
BsrI ACTGG 4 cut(s) 500, 960, 1366, 1796
BssAI RCCGGY 1 cut(s) 655
BssECI CCNNGG 6 cut(s) 248, 284, 330, 1546, 1913, 1965
BssSI CACGAG 1 cut(s) 322
BssT1I CCWWGG 1 cut(s) 1913
Bst2BI CACGAG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 1212
Bst4CI ACNGT 4 cut(s) 787, 835, 1006, 1273
Bst6I CTCTTC 3 cut(s) 177, 523, 614
BstC8I GCNNGC 3 cut(s) 868, 989, 1227
BstDEI CTNAG 6 cut(s) 235, 242, 1097, 1803, 2009, 2052
BstDSI CCRYGG 1 cut(s) 284
BstF5I GGATG 6 cut(s) 805, 1057, 1471, 1740, 1800, 2068
BstFNI CGCG 2 cut(s) 298, 991
BstHHI GCGC 4 cut(s) 300, 315, 993, 1756
BstMAI GTCTC 7 cut(s) 238, 238, 369, 571, 595, 1566, 2107
BstMCI CGRYCG 1 cut(s) 489
BstMWI GCNNNNNNNGC 9 cut(s) 81, 90, 295, 382, 621, 1226, 1231, 1373, 1438
BstNI CCWGG 1 cut(s) 1212
BstNSI RCATGY 1 cut(s) 863
BstSCI CCNGG 7 cut(s) 247, 248, 354, 451, 1210, 1546, 1965
BstSFI CTRYAG 2 cut(s) 1416, 1989
BstSLI GKGCMC 2 cut(s) 502, 778
BstUI CGCG 2 cut(s) 298, 991
BstV1I GCAGC 5 cut(s) 71, 84, 297, 622, 1794
BstV2I GAAGAC 3 cut(s) 474, 657, 1664
BstX2I RGATCY 2 cut(s) 334, 1009
BstYI RGATCY 2 cut(s) 334, 1009
Bsu36I CCTNAGG 1 cut(s) 1803
BsuRI GGCC 7 cut(s) 467, 500, 535, 543, 1367, 1933, 2044
BtgI CCRYGG 1 cut(s) 284
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 6 cut(s) 805, 1057, 1471, 1740, 1800, 2068
BtsI GCAGTG 3 cut(s) 274, 906, 1227
BtsIMutI CAGTG 6 cut(s) 274, 840, 906, 967, 1002, 1227
Cac8I GCNNGC 3 cut(s) 868, 989, 1227
CfoI GCGC 4 cut(s) 300, 315, 993, 1756
Cfr10I RCCGGY 1 cut(s) 655
Cfr13I GGNCC 9 cut(s) 352, 466, 498, 499, 533, 542, 1365, 1931, 2158
Cfr9I CCCGGG 1 cut(s) 248
CseI GACGC 2 cut(s) 577, 613
Csp6I GTAC 5 cut(s) 1323, 1461, 1489, 1881, 2078
CspCI CAANNNNNGTGG 6 cut(s) 534, 569, 816, 851, 987, 1022
CviAII CATG 4 cut(s) 471, 510, 860, 1649
CviQI GTAC 5 cut(s) 1323, 1461, 1489, 1881, 2078
DdeI CTNAG 6 cut(s) 235, 242, 1097, 1803, 2009, 2052
Eam1104I CTCTTC 3 cut(s) 177, 523, 614
EarI CTCTTC 3 cut(s) 177, 523, 614
Eco130I CCWWGG 1 cut(s) 1913
Eco24I GRGCYC 2 cut(s) 329, 502
Eco31I GGTCTC 3 cut(s) 238, 571, 1566
Eco47I GGWCC 2 cut(s) 352, 2158
Eco57I CTGAAG 2 cut(s) 583, 1132
Eco81I CCTNAGG 1 cut(s) 1803
Eco88I CYCGRG 3 cut(s) 248, 329, 2115
EcoO109I RGGNCCY 2 cut(s) 352, 1365
EcoRI GAATTC 1 cut(s) 2003
EcoRII CCWGG 1 cut(s) 1210
EcoT14I CCWWGG 1 cut(s) 1913
EcoT22I ATGCAT 2 cut(s) 1254, 1705
EcoT38I GRGCYC 2 cut(s) 329, 502
ErhI CCWWGG 1 cut(s) 1913
Esp3I CGTCTC 1 cut(s) 595
FaeI CATG 4 cut(s) 474, 513, 863, 1652
FaqI GGGAC 2 cut(s) 338, 1086
FatI CATG 4 cut(s) 470, 509, 859, 1648
FauI CCCGC 5 cut(s) 695, 707, 996, 1625, 2041
FbaI TGATCA 3 cut(s) 1513, 2065, 2092
FokI GGATG 6 cut(s) 792, 1064, 1458, 1747, 1807, 2055
FriOI GRGCYC 2 cut(s) 329, 502
FspAI RTGCGCAY 1 cut(s) 1755
FspBI CTAG 2 cut(s) 759, 768
FspI TGCGCA 1 cut(s) 1755
GlaI GCGC 4 cut(s) 299, 314, 992, 1755
GsaI CCCAGC 1 cut(s) 534
GsuI CTGGAG 1 cut(s) 1233
HaeIII GGCC 7 cut(s) 467, 500, 535, 543, 1367, 1933, 2044
HapII CCGG 9 cut(s) 249, 356, 453, 563, 574, 656, 908, 1548, 1967
HgaI GACGC 2 cut(s) 577, 613
HhaI GCGC 4 cut(s) 300, 315, 993, 1756
Hin1II CATG 4 cut(s) 474, 513, 863, 1652
Hin6I GCGC 4 cut(s) 298, 313, 991, 1754
HinP1I GCGC 4 cut(s) 298, 313, 991, 1754
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 5 cut(s) 4, 341, 425, 461, 1595
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 9 cut(s) 249, 356, 453, 563, 574, 656, 908, 1548, 1967
HphI GGTGA 8 cut(s) 125, 503, 645, 897, 1071, 1184, 1535, 1847
Hpy166II GTNNAC 4 cut(s) 277, 1142, 1543, 2198
Hpy188I TCNGA 4 cut(s) 36, 243, 1316, 1571
Hpy188III TCNNGA 9 cut(s) 322, 338, 563, 1027, 1037, 1049, 1400, 1802, 1891
Hpy8I GTNNAC 4 cut(s) 277, 1142, 1543, 2198
Hpy99I CGWCG 3 cut(s) 53, 629, 2158
HpyCH4III ACNGT 4 cut(s) 787, 835, 1006, 1273
HpyCH4IV ACGT 1 cut(s) 925
HpyF10VI GCNNNNNNNGC 9 cut(s) 81, 90, 295, 382, 621, 1226, 1231, 1373, 1438
HpyF3I CTNAG 6 cut(s) 235, 242, 1097, 1803, 2009, 2052
HpySE526I ACGT 1 cut(s) 925
Hsp92II CATG 4 cut(s) 474, 513, 863, 1652
HspAI GCGC 4 cut(s) 298, 313, 991, 1754
KflI GGGWCCC 1 cut(s) 352
Kpn2I TCCGGA 1 cut(s) 562
KpnI GGTACC 1 cut(s) 1326
Ksp22I TGATCA 3 cut(s) 1513, 2065, 2092
KspAI GTTAAC 1 cut(s) 277
LguI GCTCTTC 1 cut(s) 614
LmnI GCTCC 8 cut(s) 163, 518, 958, 1214, 1438, 1583, 1804, 1869
Lsp1109I GCAGC 5 cut(s) 71, 84, 297, 622, 1794
LweI GCATC 6 cut(s) 668, 676, 1261, 1480, 1690, 1712
MaeI CTAG 2 cut(s) 759, 768
MaeII ACGT 1 cut(s) 925
MaeIII GTNAC 5 cut(s) 491, 855, 903, 1077, 1267
MfeI CAATTG 1 cut(s) 1433
MflI RGATCY 2 cut(s) 334, 1009
MhlI GDGCHC 4 cut(s) 329, 502, 778, 955
MlyI GAGTC 3 cut(s) 335, 434, 470
MmeI TCCRAC 1 cut(s) 980
Mph1103I ATGCAT 2 cut(s) 1254, 1705
MroI TCCGGA 1 cut(s) 562
MroXI GAANNNNTTC 1 cut(s) 1539
MseI TTAA 5 cut(s) 276, 729, 1449, 1677, 2166
MslI CAYNNNNRTG 2 cut(s) 1749, 1892
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 9 cut(s) 249, 356, 453, 563, 574, 656, 908, 1548, 1967
MspR9I CCNGG 7 cut(s) 249, 250, 356, 453, 1212, 1548, 1967
MunI CAATTG 1 cut(s) 1433
Mva1269I GAATGC 3 cut(s) 30, 1756, 2190
MvaI CCWGG 1 cut(s) 1212
MvnI CGCG 2 cut(s) 298, 991
MwoI GCNNNNNNNGC 9 cut(s) 81, 90, 295, 382, 621, 1226, 1231, 1373, 1438
NciI CCSGG 6 cut(s) 249, 250, 356, 453, 1548, 1967
NlaIII CATG 4 cut(s) 474, 513, 863, 1652
NmeAIII GCCGAG 1 cut(s) 707
NmuCI GTSAC 4 cut(s) 491, 855, 903, 1077
NsbI TGCGCA 1 cut(s) 1755
NsiI ATGCAT 2 cut(s) 1254, 1705
NspI RCATGY 1 cut(s) 863
PciI ACATGT 1 cut(s) 859
PciSI GCTCTTC 1 cut(s) 614
PctI GAATGC 3 cut(s) 30, 1756, 2190
PdmI GAANNNNTTC 1 cut(s) 1539
PfeI GAWTC 1 cut(s) 1595
Pfl23II CGTACG 1 cut(s) 1460
PflFI GACNNNGTC 1 cut(s) 587
PfoI TCCNGGA 2 cut(s) 354, 1210
PleI GAGTC 3 cut(s) 335, 433, 469
PpsI GAGTC 3 cut(s) 335, 433, 469
PpuMI RGGWCCY 1 cut(s) 352
PscI ACATGT 1 cut(s) 859
PsiI TTATAA 1 cut(s) 879
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 1 cut(s) 1210
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 1 cut(s) 1210
PspLI CGTACG 1 cut(s) 1460
PspOMI GGGCCC 1 cut(s) 498
PspPI GGNCC 9 cut(s) 352, 466, 498, 499, 533, 542, 1365, 1931, 2158
PspPPI RGGWCCY 1 cut(s) 352
PsrI GAACNNNNNNTAC 2 cut(s) 175, 207
PstI CTGCAG 2 cut(s) 1420, 1993
PsuI RGATCY 2 cut(s) 334, 1009
PsyI GACNNNGTC 1 cut(s) 587
PvuII CAGCTG 1 cut(s) 84
RsaI GTAC 5 cut(s) 1324, 1462, 1490, 1882, 2079
RsaNI GTAC 5 cut(s) 1323, 1461, 1489, 1881, 2078
RseI CAYNNNNRTG 2 cut(s) 1749, 1892
SapI GCTCTTC 1 cut(s) 614
SaqAI TTAA 5 cut(s) 276, 729, 1449, 1677, 2166
Sau96I GGNCC 9 cut(s) 352, 466, 498, 499, 533, 542, 1365, 1931, 2158
ScaI AGTACT 1 cut(s) 1490
SchI GAGTC 3 cut(s) 335, 434, 470
ScrFI CCNGG 7 cut(s) 249, 250, 356, 453, 1212, 1548, 1967
SduI GDGCHC 4 cut(s) 329, 502, 778, 955
SfaNI GCATC 6 cut(s) 668, 676, 1261, 1480, 1690, 1712
SfcI CTRYAG 2 cut(s) 1416, 1989
SgrAI CRCCGGYG 1 cut(s) 655
SinI GGWCC 2 cut(s) 352, 2158
SmaI CCCGGG 1 cut(s) 250
SmiMI CAYNNNNRTG 2 cut(s) 1749, 1892
SmlI CTYRAG 4 cut(s) 537, 796, 940, 970
SmoI CTYRAG 4 cut(s) 537, 796, 940, 970
SspI AATATT 2 cut(s) 392, 1480
SspMI CTAG 2 cut(s) 759, 768
StyD4I CCNGG 7 cut(s) 247, 248, 354, 451, 1210, 1546, 1965
StyI CCWWGG 1 cut(s) 1913
TaaI ACNGT 4 cut(s) 787, 835, 1006, 1273
TaiI ACGT 1 cut(s) 928
TaqI TCGA 6 cut(s) 339, 395, 627, 1135, 1890, 2182
TaqII GACCGA 2 cut(s) 468, 475
TatI WGTACW 1 cut(s) 1488
TauI GCSGC 5 cut(s) 267, 292, 559, 1220, 1237
TfiI GAWTC 1 cut(s) 1595
Tru1I TTAA 5 cut(s) 276, 729, 1449, 1677, 2166
Tru9I TTAA 5 cut(s) 276, 729, 1449, 1677, 2166
TscAI CASTG 6 cut(s) 274, 840, 906, 967, 1009, 1227
TseFI GTSAC 4 cut(s) 491, 855, 903, 1077
TseI GCWGC 5 cut(s) 72, 84, 310, 635, 1782
Tsp45I GTSAC 4 cut(s) 491, 855, 903, 1077
TspDTI ATGAA 4 cut(s) 189, 1298, 1394, 1749
TspGWI ACGGA 4 cut(s) 405, 1448, 1588, 2171
TspMI CCCGGG 1 cut(s) 248
TspRI CASTG 6 cut(s) 274, 840, 906, 967, 1009, 1227
Tth111I GACNNNGTC 1 cut(s) 587
VpaK11BI GGWCC 2 cut(s) 352, 2158
XapI RAATTY 5 cut(s) 436, 1687, 1922, 2003, 2082
XceI RCATGY 1 cut(s) 863
XcmI CCANNNNNNNNNTGG 1 cut(s) 1628
XmaI CCCGGG 1 cut(s) 248
XmnI GAANNNNTTC 1 cut(s) 1539
XspI CTAG 2 cut(s) 759, 768
ZrmI AGTACT 1 cut(s) 1490
Zsp2I ATGCAT 2 cut(s) 1254, 1705
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.