pycom15g03340

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001271
Physical Location & Seq
Forward (+)
2065205 .. 2068722
3518 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g03340.8

Sequence Viewer

Length: 1833 bp
ATGCCTTCTACTTCGTTTTCTCATCACAACCCCTGCAGAGCTACAACCTCCTCCTCCTCCCCTGTTTTTTCTTCAGCTGCTGCTTGCTCTGTTTCTGCTCCGAAACAGGACAGTGGTGGTGGTGGGGGCAGCTGCGAGCAGAGCCCGCGGCGGCTCACCCGGCAAAGAAAGCTCCGCCACATCAACAACCAGGATTTCTACGTCCCTGAGAAGTCTTACTCCTCGCCCGCTTCGCCGGAATGTTCGACCCAAAAGTCAAGATCGCCGGGTCACGGGTCCAAGCCAAACCACTGGTCTTCTGCGCCGGCTCCGCAGCCCTTGCCCCTTCCTGAATCTCCCGTGATCCGGAAACCTGAATCTGCTAAAAATGGGTTCCTTGGCCCCAGGAAGGTCAAAGGTAAAGCTGTCACAAGCTCAGGATCGGCATCGCCGAGTTCTTATTCAAGTCAAGTTGCCACAACCTCTGGAGGATCAGCAGCAGCAGTGAGTTTTTATTCAAACAATCGTCGGGGGGCACTCTCCCAAGATGCTAACAATGGTGGCGGTTCGCTGAGTTCTTATTCTAGTCAAGTTGCCACAACCTCAGGAGGATCATCAGCACCAGTGCGTTCTTATTCGAACAATCGTCGAGGGGCACTCTCCCAAGATGCTAACAATGGTGGGGGTTTCTTTAATAACTTGGCTCTGAATATTCTTTCTAGGAAAGCGCAGGCAAATGGTATATCAAGCCATCCTTTTAGCCCCCAAAGATCGAAAACTGTCGATAGTTATCCGATACTTGTTGCTCCCACTTCACCCGTTAATTGTTCAAGCAACTGCCATAGGGGAACGCCACACGAATATGAAAGTTGCAACATGAGCAGGAGTGCTCCAACAAGTGCTTTTTCAAGTCCTGCAGTTAGCCCCAGGAGATCAAACACCGCTGGGGGTTTTTTTACTTCTTCTGGGGCTTCCCAAGATCAAAATCTGGATGTCTTTCCTCCAAGATTATCACCAAATAAACCTTCTAATAGTCCCAGCCATTCTCCTACATCTCGAAGTCCACGCTTCAACCAGAAAAGTTCAAATGGAAATGCATTTCTTTTGCCTTCTTTAGAGAGGGTTGAAAGTAGTGGTCAAGTCAGTGCCCACCCTTTGCCTCTGCCACCTCCACCACAGCAATCTGTTATGCACCACAATGCAGAAGCTCTATCGTTATCATCAATGAAAGGTCAATGGCAGAAGGGAAAACTTATTGGGCGCGGCACCTTTGGTAGTGTGTATCTCGCAACCAATCGAGAAACCGGGGCCTTGTGTGCGATGAAGGAAGTTGACCTCATTCCTGATGATCCTAAAGCTGCTGAATGTATAAAGCAGTTGGAGCAGGAAATTAAAGTCCTCCGCACACTAAAGCATCCAAATATTGTGCAGTATTATGGTAGTGAAATGGTGAGCGTTGTTAAACTTGCCGATTTTGGGGTGGCAAAACATCTCAATGGGCAATCGTACAATCTTTCTTTGAAGGGCAGTCCATACTGGATGGCTCCTGAGGTGATAAGGGCTGCAATGCAGAACAGTGCTGCTCCTGATCTTGCTTTGGCCGTCGATATATGGAGCTTGGGTTGTACTATTGTTGAGATGTTCAATGGAAAACCTCCGTGGAGTGAATTTACAGGACCTCAAGCTATGTTCAAGGTTCTAAACACCATCCCGGATATACCAGAAACATTATCTGCAGAAGGAAAGGACTTCCTTCATTTGTGCTTTCGTAGGAACCCTGCAGAGAGACCGTTAGCTAGTAAGCTACTTGAGCATCCGTTTGTACGAAATTCACACATCAGACCTTTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

611

Amino Acids

65.1

Weight (kDa)

9.69

Isoelectric Point (pI)

67.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 253
AccB1I GGYRCC 1 cut(s) 1244
AccII CGCG 2 cut(s) 148, 1242
AccIII TCCGGA 1 cut(s) 345
AclWI GGATC 5 cut(s) 337, 427, 478, 598, 1322
AcoI YGGCCR 1 cut(s) 1578
AcsI RAATTY 2 cut(s) 1646, 1807
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 3 cut(s) 1487, 1606, 1803
AfiI CCNNNNNNNGG 4 cut(s) 272, 345, 388, 1455
AjnI CCWGG 3 cut(s) 189, 383, 905
AjuI GAANNNNNNNTTGG 2 cut(s) 988, 1020
Alw21I GWGCWC 1 cut(s) 871
Alw26I GTCTC 1 cut(s) 1759
AlwI GGATC 5 cut(s) 337, 427, 478, 598, 1322
AlwNI CAGNNNCTG 1 cut(s) 80
Aor13HI TCCGGA 1 cut(s) 345
AoxI GGCC 3 cut(s) 379, 1287, 1578
ApoI RAATTY 2 cut(s) 1646, 1807
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AspLEI GCGC 3 cut(s) 304, 709, 1242
AspS9I GGNCC 4 cut(s) 276, 380, 1287, 1655
AsuC2I CCSGG 4 cut(s) 160, 267, 1285, 1691
AsuHPI GGTGA 5 cut(s) 148, 786, 984, 1441, 1543
AsuII TTCGAA 1 cut(s) 617
AvaII GGWCC 2 cut(s) 276, 1655
AxyI CCTNAGG 2 cut(s) 583, 1527
BaeGI GKGCMC 3 cut(s) 517, 637, 1129
BanI GGYRCC 1 cut(s) 1244
BanII GRGCYC 1 cut(s) 146
BbsI GAAGAC 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 871
BccI CCATC 3 cut(s) 738, 1513, 1694
BceAI ACGGC 1 cut(s) 1565
BciT130I CCWGG 3 cut(s) 191, 385, 907
BcnI CCSGG 4 cut(s) 160, 267, 1285, 1691
BcoDI GTCTC 1 cut(s) 1759
BfaI CTAG 3 cut(s) 564, 699, 1776
BfmI CTRYAG 4 cut(s) 34, 894, 1713, 1758
Bme1390I CCNGG 7 cut(s) 160, 191, 267, 385, 907, 1285, 1691
Bme18I GGWCC 2 cut(s) 276, 1655
BmgT120I GGNCC 4 cut(s) 276, 380, 1287, 1655
BmiI GGNNCC 8 cut(s) 277, 309, 374, 382, 1246, 1288, 1524, 1754
BmrFI CCNGG 7 cut(s) 160, 191, 267, 385, 907, 1285, 1691
BmsI GCATC 5 cut(s) 434, 517, 637, 1402, 1801
BpiI GAAGAC 1 cut(s) 288
BplI GAGNNNNNCTC 2 cut(s) 621, 653
BpmI CTGGAG 1 cut(s) 486
Bpu10I CCTNAGC 1 cut(s) 415
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 2 cut(s) 1644, 1808
BpuMI CCSGG 4 cut(s) 160, 267, 1285, 1691
BsaBI GATNNNNATC 3 cut(s) 424, 768, 963
BsaI GGTCTC 1 cut(s) 1759
BsaJI CCNNGG 6 cut(s) 146, 376, 383, 905, 1284, 1637
BsaWI WCCGGW 1 cut(s) 345
BsaXI ACNNNNNCTCC 2 cut(s) 1585, 1615
Bsc4I CCNNNNNNNGG 4 cut(s) 272, 345, 388, 1455
Bse118I RCCGGY 1 cut(s) 304
Bse1I ACTGG 3 cut(s) 296, 602, 1520
Bse21I CCTNAGG 2 cut(s) 583, 1527
Bse3DI GCAATG 1 cut(s) 1551
Bse8I GATNNNNATC 3 cut(s) 424, 768, 963
BseAI TCCGGA 1 cut(s) 345
BseBI CCWGG 3 cut(s) 191, 385, 907
BseDI CCNNGG 6 cut(s) 146, 376, 383, 905, 1284, 1637
BseGI GGATG 6 cut(s) 730, 976, 1393, 1524, 1686, 1792
BseJI GATNNNNATC 3 cut(s) 424, 768, 963
BseLI CCNNNNNNNGG 4 cut(s) 272, 345, 388, 1455
BseMI GCAATG 1 cut(s) 1551
BseMII CTCAG 5 cut(s) 198, 429, 542, 597, 1518
BseNI ACTGG 3 cut(s) 296, 602, 1520
BseRI GAGGAG 4 cut(s) 40, 43, 46, 211
BseSI GKGCMC 3 cut(s) 517, 637, 1129
BseYI CCCAGC 2 cut(s) 923, 1016
BsgI GTGCAG 1 cut(s) 1427
Bsh1236I CGCG 2 cut(s) 148, 1242
BshFI GGCC 3 cut(s) 381, 1289, 1580
BshNI GGYRCC 1 cut(s) 1244
BsiHKAI GWGCWC 1 cut(s) 871
BsiSI CCGG 7 cut(s) 160, 236, 266, 305, 346, 1284, 1691
BslFI GGGAC 2 cut(s) 188, 999
BslI CCNNNNNNNGG 4 cut(s) 272, 345, 388, 1455
BsmAI GTCTC 1 cut(s) 1759
BsmFI GGGAC 2 cut(s) 188, 999
BsnI GGCC 3 cut(s) 381, 1289, 1580
Bso31I GGTCTC 1 cut(s) 1759
Bsp119I TTCGAA 1 cut(s) 617
Bsp1286I GDGCHC 5 cut(s) 146, 517, 637, 871, 1129
Bsp13I TCCGGA 1 cut(s) 345
BspANI GGCC 3 cut(s) 381, 1289, 1580
BspCNI CTCAG 5 cut(s) 199, 428, 543, 596, 1519
BspEI TCCGGA 1 cut(s) 345
BspFNI CGCG 2 cut(s) 148, 1242
BspLI GGNNCC 8 cut(s) 277, 309, 374, 382, 1246, 1288, 1524, 1754
BspMAI CTGCAG 4 cut(s) 38, 898, 1717, 1762
BspPI GGATC 5 cut(s) 337, 427, 478, 598, 1322
BspT104I TTCGAA 1 cut(s) 617
BspT107I GGYRCC 1 cut(s) 1244
BspTNI GGTCTC 1 cut(s) 1759
BsrDI GCAATG 1 cut(s) 1551
BsrFI RCCGGY 1 cut(s) 304
BsrI ACTGG 3 cut(s) 296, 602, 1520
BssAI RCCGGY 1 cut(s) 304
BssECI CCNNGG 6 cut(s) 146, 376, 383, 905, 1284, 1637
BssT1I CCWWGG 1 cut(s) 376
Bst2UI CCWGG 3 cut(s) 191, 385, 907
Bst4CI ACNGT 4 cut(s) 113, 760, 1556, 1770
BstAPI GCANNNNNTGC 1 cut(s) 319
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 6 cut(s) 85, 137, 146, 228, 306, 711
BstDEI CTNAG 5 cut(s) 207, 415, 551, 583, 1527
BstDSI CCRYGG 2 cut(s) 146, 1637
BstF5I GGATG 6 cut(s) 730, 976, 1393, 1524, 1686, 1792
BstFNI CGCG 2 cut(s) 148, 1242
BstHHI GCGC 3 cut(s) 304, 709, 1242
BstMAI GTCTC 1 cut(s) 1759
BstNI CCWGG 3 cut(s) 191, 385, 907
BstSCI CCNGG 7 cut(s) 158, 189, 265, 383, 905, 1283, 1689
BstSFI CTRYAG 4 cut(s) 34, 894, 1713, 1758
BstSLI GKGCMC 3 cut(s) 517, 637, 1129
BstUI CGCG 2 cut(s) 148, 1242
BstV2I GAAGAC 1 cut(s) 288
BstXI CCANNNNNNTGG 1 cut(s) 291
Bsu36I CCTNAGG 2 cut(s) 583, 1527
BsuRI GGCC 3 cut(s) 381, 1289, 1580
BtgI CCRYGG 2 cut(s) 146, 1637
BtgZI GCGATG 2 cut(s) 411, 1313
BtsCI GGATG 6 cut(s) 730, 976, 1393, 1524, 1686, 1792
BtsI GCAGTG 1 cut(s) 489
BtsIMutI CAGTG 6 cut(s) 118, 289, 489, 609, 1129, 1561
Cac8I GCNNGC 6 cut(s) 85, 137, 146, 228, 306, 711
CaiI CAGNNNCTG 1 cut(s) 80
CfoI GCGC 3 cut(s) 304, 709, 1242
Cfr10I RCCGGY 1 cut(s) 304
Cfr13I GGNCC 4 cut(s) 276, 380, 1287, 1655
Cfr42I CCGCGG 1 cut(s) 149
Csp6I GTAC 3 cut(s) 1486, 1605, 1802
CviAII CATG 1 cut(s) 856
CviQI GTAC 3 cut(s) 1486, 1605, 1802
DdeI CTNAG 5 cut(s) 207, 415, 551, 583, 1527
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 1 cut(s) 1578
EciI GGCGGA 1 cut(s) 164
Eco130I CCWWGG 1 cut(s) 376
Eco24I GRGCYC 1 cut(s) 146
Eco31I GGTCTC 1 cut(s) 1759
Eco47I GGWCC 2 cut(s) 276, 1655
Eco57I CTGAAG 1 cut(s) 57
Eco81I CCTNAGG 2 cut(s) 583, 1527
EcoO109I RGGNCCY 2 cut(s) 1287, 1655
EcoRII CCWGG 3 cut(s) 189, 383, 905
EcoT14I CCWWGG 1 cut(s) 376
EcoT22I ATGCAT 1 cut(s) 1078
EcoT38I GRGCYC 1 cut(s) 146
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 1 cut(s) 859
FalI AAGNNNNNCTT 2 cut(s) 718, 750
FaqI GGGAC 2 cut(s) 188, 999
FatI CATG 1 cut(s) 855
FauI CCCGC 2 cut(s) 153, 235
FokI GGATG 6 cut(s) 717, 983, 1380, 1531, 1673, 1779
FriOI GRGCYC 1 cut(s) 146
FspBI CTAG 3 cut(s) 564, 699, 1776
GlaI GCGC 3 cut(s) 303, 708, 1241
GsaI CCCAGC 2 cut(s) 927, 1020
GsuI CTGGAG 1 cut(s) 486
HaeIII GGCC 3 cut(s) 381, 1289, 1580
HapII CCGG 7 cut(s) 160, 236, 266, 305, 346, 1284, 1691
HhaI GCGC 3 cut(s) 304, 709, 1242
Hin1II CATG 1 cut(s) 859
Hin6I GCGC 3 cut(s) 302, 707, 1240
HinP1I GCGC 3 cut(s) 302, 707, 1240
HincII GTYRAC 1 cut(s) 1312
HindII GTYRAC 1 cut(s) 1312
HinfI GANTC 2 cut(s) 332, 356
HpaII CCGG 7 cut(s) 160, 236, 266, 305, 346, 1284, 1691
HphI GGTGA 5 cut(s) 148, 786, 984, 1441, 1543
Hpy166II GTNNAC 2 cut(s) 1043, 1312
Hpy188I TCNGA 4 cut(s) 102, 687, 774, 1820
Hpy8I GTNNAC 2 cut(s) 1043, 1312
Hpy99I CGWCG 3 cut(s) 510, 630, 1586
HpyCH4III ACNGT 4 cut(s) 113, 760, 1556, 1770
HpyCH4IV ACGT 1 cut(s) 201
HpyF3I CTNAG 5 cut(s) 207, 415, 551, 583, 1527
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 1 cut(s) 859
HspAI GCGC 3 cut(s) 302, 707, 1240
Kpn2I TCCGGA 1 cut(s) 345
KroI GCCGGC 1 cut(s) 304
KroNI GCCGGC 1 cut(s) 306
KspI CCGCGG 1 cut(s) 149
LmnI GCTCC 9 cut(s) 103, 177, 313, 790, 874, 1360, 1528, 1567, 1593
LweI GCATC 5 cut(s) 434, 517, 637, 1402, 1801
MaeI CTAG 3 cut(s) 564, 699, 1776
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 2 cut(s) 269, 406
MboII GAAGA 3 cut(s) 63, 288, 933
MhlI GDGCHC 5 cut(s) 146, 517, 637, 871, 1129
MluCI AATT 4 cut(s) 802, 1368, 1646, 1807
MmeI TCCRAC 2 cut(s) 896, 1338
Mph1103I ATGCAT 1 cut(s) 1078
MroI TCCGGA 1 cut(s) 345
MroNI GCCGGC 1 cut(s) 304
MseI TTAA 4 cut(s) 672, 801, 1371, 1440
MslI CAYNNNNRTG 3 cut(s) 840, 1176, 1473
MspA1I CMGCKG 4 cut(s) 77, 132, 148, 923
MspI CCGG 7 cut(s) 160, 236, 266, 305, 346, 1284, 1691
MspR9I CCNGG 7 cut(s) 160, 191, 267, 385, 907, 1285, 1691
MvaI CCWGG 3 cut(s) 191, 385, 907
MvnI CGCG 2 cut(s) 148, 1242
NaeI GCCGGC 1 cut(s) 306
NciI CCSGG 4 cut(s) 160, 267, 1285, 1691
NgoMIV GCCGGC 1 cut(s) 304
NlaIII CATG 1 cut(s) 859
NlaIV GGNNCC 8 cut(s) 277, 309, 374, 382, 1246, 1288, 1524, 1754
NmeAIII GCCGAG 1 cut(s) 456
NmuCI GTSAC 2 cut(s) 269, 406
NsiI ATGCAT 1 cut(s) 1078
NspV TTCGAA 1 cut(s) 617
PcsI WCGNNNNNNNCGW 1 cut(s) 428
PdiI GCCGGC 1 cut(s) 306
PfeI GAWTC 2 cut(s) 332, 356
PfoI TCCNGGA 1 cut(s) 1689
PpuMI RGGWCCY 1 cut(s) 1655
Psp5II RGGWCCY 1 cut(s) 1655
Psp6I CCWGG 3 cut(s) 189, 383, 905
PspFI CCCAGC 2 cut(s) 923, 1016
PspGI CCWGG 3 cut(s) 189, 383, 905
PspN4I GGNNCC 8 cut(s) 277, 309, 374, 382, 1246, 1288, 1524, 1754
PspPI GGNCC 4 cut(s) 276, 380, 1287, 1655
PspPPI RGGWCCY 1 cut(s) 1655
PstI CTGCAG 4 cut(s) 38, 898, 1717, 1762
PstNI CAGNNNCTG 1 cut(s) 80
PvuII CAGCTG 2 cut(s) 77, 132
RsaI GTAC 3 cut(s) 1487, 1606, 1803
RsaNI GTAC 3 cut(s) 1486, 1605, 1802
RseI CAYNNNNRTG 3 cut(s) 840, 1176, 1473
SacII CCGCGG 1 cut(s) 149
SaqAI TTAA 4 cut(s) 672, 801, 1371, 1440
Sau96I GGNCC 4 cut(s) 276, 380, 1287, 1655
ScrFI CCNGG 7 cut(s) 160, 191, 267, 385, 907, 1285, 1691
SduI GDGCHC 5 cut(s) 146, 517, 637, 871, 1129
SfaNI GCATC 5 cut(s) 434, 517, 637, 1402, 1801
SfcI CTRYAG 4 cut(s) 34, 894, 1713, 1758
Sfr303I CCGCGG 1 cut(s) 149
SfuI TTCGAA 1 cut(s) 617
SgrBI CCGCGG 1 cut(s) 149
SinI GGWCC 2 cut(s) 276, 1655
SmiMI CAYNNNNRTG 3 cut(s) 840, 1176, 1473
SmlI CTYRAG 2 cut(s) 1659, 1787
SmoI CTYRAG 2 cut(s) 1659, 1787
Sse9I AATT 4 cut(s) 802, 1368, 1646, 1807
SspI AATATT 2 cut(s) 691, 1402
SspMI CTAG 3 cut(s) 564, 699, 1776
StyD4I CCNGG 7 cut(s) 158, 189, 265, 383, 905, 1283, 1689
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 4 cut(s) 113, 760, 1556, 1770
TaiI ACGT 1 cut(s) 204
TaqI TCGA 8 cut(s) 245, 617, 628, 752, 762, 1036, 1276, 1584
TasI AATT 4 cut(s) 802, 1368, 1646, 1807
TatI WGTACW 1 cut(s) 1604
TauI GCSGC 3 cut(s) 151, 154, 1245
TfiI GAWTC 2 cut(s) 332, 356
Tru1I TTAA 4 cut(s) 672, 801, 1371, 1440
Tru9I TTAA 4 cut(s) 672, 801, 1371, 1440
TscAI CASTG 6 cut(s) 118, 296, 489, 609, 1129, 1561
TseFI GTSAC 2 cut(s) 269, 406
Tsp45I GTSAC 2 cut(s) 269, 406
TspDTI ATGAA 4 cut(s) 858, 1220, 1316, 1724
TspGWI ACGGA 2 cut(s) 1626, 1785
TspRI CASTG 6 cut(s) 118, 296, 489, 609, 1129, 1561
VpaK11BI GGWCC 2 cut(s) 276, 1655
XapI RAATTY 2 cut(s) 1646, 1807
XspI CTAG 3 cut(s) 564, 699, 1776
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.