Rorug02G0123100

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001271
Physical Location & Seq
Reverse (-)
10588506 .. 10589911
1406 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0123100.1

Sequence Viewer

Length: 990 bp
ATGTTAAGTGTTCTCATTGAGAAATCCCTGTTAACCATGTTTGACAACTGTGTGAATATGCATGATTTGATACAAGAAATGGGAAGACAAATTGTCCGTTTGGAGTCTAAAAAAAAGCCTGGGCAACACAGTCATTTGTGGCTTCGTGACGACATCTTTCGTGTATTGAAACAAAATACGGGAACAGAAACAATCAGAAGCATAGCCTTATGCTTGCCTAAAGTGGAAGAGTTAGCTTGGAATCCCAATGCCTTCCATATGATGTCTGAACTGAAGTTTCTCAAGATTCAAAATTTGGTTCTATTTCAAGGCCCTAAATGTCTTCCTGATGACATAAGGGTCCTCGAATGGAATTTGTATCCATCAAAGTCTCTTCCAGCAGATTTTCAGCCGGTGGAACTTTTTGAGCTTACTCTGCATCATAGCAAAATTGATCAGATTTGGCATGGAAGAAAGTGCCGAAGCATTAAGAGTCTTCCAAGTGAATTAGAAATTGATTCTCTTGAAAGAGTTGATCTTTCTGGCTGCTCAAATGTGGATATATTTCCACGATTCGTGTTGCAGATGAAAACATTATCAGAACTTTCTTTTGGTGGAATTGGCATGCAAAGTTCCGATGTATCATCATGTTATTCAGTAGGTCATCATCACCCACTGAGTCCGGGATTAAATCTAAATGGCTGCAAACTAGGTGAAGGAGCAATTCCTGATGATATTGGTTGTTTGTCCTCCGTAGAAGAATTGGATCTTAGTGTTAACAATTTTGCCAGCCTTCCTGCAAGCATTAGTCGGCTATCGAAGCTCAAGTGTTTAAACTTGGCAGATTGTAAAAGGCTTAAACAATTGCCACGCCTTCCATCATTTAGTGGCACATGTCATGTTACCGCGGACAACTGTATATCCATATCCTTAAAAAAGTTTCCAGATCCACCAGACTTGTGTGCAAATTATGGAAACTATCCTTCAACTTTATCAATTGCTGCAGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

329

Amino Acids

36.73

Weight (kDa)

6.59

Isoelectric Point (pI)

39.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_ROQ1 PF23282 2 - 35 1.1e-07 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 233 - 283 1e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AccII CGCG 1 cut(s) 887
AciI CCGC 2 cut(s) 885, 887
AclWI GGATC 2 cut(s) 753, 920
AcsI RAATTY 2 cut(s) 292, 352
AcuI CTGAAG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 872
AgsI TTSAA 5 cut(s) 169, 290, 308, 506, 966
AhdI GACNNNNNGTC 1 cut(s) 92
AjnI CCWGG 1 cut(s) 118
AjuI GAANNNNNNNTTGG 2 cut(s) 573, 605
AluBI AGCT 3 cut(s) 236, 409, 802
AluI AGCT 3 cut(s) 236, 409, 802
Alw26I GTCTC 1 cut(s) 375
AlwI GGATC 2 cut(s) 753, 920
AoxI GGCC 1 cut(s) 310
ApeKI GCWGC 4 cut(s) 525, 681, 980, 983
ApoI RAATTY 2 cut(s) 292, 352
AspS9I GGNCC 2 cut(s) 311, 340
AsuC2I CCSGG 1 cut(s) 663
AsuHPI GGTGA 2 cut(s) 641, 704
AvaII GGWCC 1 cut(s) 340
BbsI GAAGAC 3 cut(s) 91, 314, 467
BbvI GCAGC 3 cut(s) 512, 668, 967
BccI CCATC 2 cut(s) 370, 865
BciT130I CCWGG 1 cut(s) 120
BciVI GTATCC 1 cut(s) 369
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 1 cut(s) 663
BcoDI GTCTC 1 cut(s) 375
BfaI CTAG 1 cut(s) 689
BfmI CTRYAG 1 cut(s) 981
BfuI GTATCC 1 cut(s) 369
BisI GCNGC 4 cut(s) 526, 682, 981, 984
BlsI GCNGC 4 cut(s) 527, 683, 982, 985
Bme1390I CCNGG 2 cut(s) 120, 663
Bme18I GGWCC 1 cut(s) 340
BmeRI GACNNNNNGTC 1 cut(s) 92
BmgT120I GGNCC 2 cut(s) 311, 340
BmiI GGNNCC 1 cut(s) 341
BmrFI CCNGG 2 cut(s) 120, 663
BmsI GCATC 1 cut(s) 427
BpiI GAAGAC 3 cut(s) 91, 314, 467
BpuEI CTTGAG 2 cut(s) 266, 788
BpuMI CCSGG 1 cut(s) 663
BsaJI CCNNGG 2 cut(s) 119, 885
Bse118I RCCGGY 1 cut(s) 391
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 119, 885
BseMII CTCAG 1 cut(s) 647
BseXI GCAGC 3 cut(s) 512, 668, 967
Bsh1236I CGCG 1 cut(s) 887
BshFI GGCC 1 cut(s) 312
BsiSI CCGG 2 cut(s) 392, 662
BsmAI GTCTC 1 cut(s) 375
BsnI GGCC 1 cut(s) 312
Bsp143I GATC 4 cut(s) 433, 514, 745, 925
BspACI CCGC 2 cut(s) 885, 887
BspANI GGCC 1 cut(s) 312
BspCNI CTCAG 1 cut(s) 648
BspFNI CGCG 1 cut(s) 887
BspLI GGNNCC 1 cut(s) 341
BspMAI CTGCAG 1 cut(s) 985
BspPI GGATC 2 cut(s) 753, 920
BsrFI RCCGGY 1 cut(s) 391
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 2 cut(s) 119, 885
BssMI GATC 4 cut(s) 433, 514, 745, 925
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 50, 131, 896
Bst6I CTCTTC 2 cut(s) 222, 378
BstC8I GCNNGC 4 cut(s) 215, 605, 769, 781
BstDEI CTNAG 2 cut(s) 656, 749
BstDSI CCRYGG 1 cut(s) 885
BstFNI CGCG 1 cut(s) 887
BstKTI GATC 4 cut(s) 436, 517, 748, 928
BstMAI GTCTC 1 cut(s) 375
BstMBI GATC 4 cut(s) 433, 514, 745, 925
BstMWI GCNNNNNNNGC 2 cut(s) 415, 799
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 2 cut(s) 607, 876
BstSCI CCNGG 2 cut(s) 118, 661
BstSFI CTRYAG 1 cut(s) 981
BstUI CGCG 1 cut(s) 887
BstV1I GCAGC 3 cut(s) 512, 668, 967
BstV2I GAAGAC 3 cut(s) 91, 314, 467
BstX2I RGATCY 2 cut(s) 745, 925
BstYI RGATCY 2 cut(s) 745, 925
BsuI GTATCC 1 cut(s) 369
BsuRI GGCC 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 885
BtsIMutI CAGTG 1 cut(s) 653
Cac8I GCNNGC 4 cut(s) 215, 605, 769, 781
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 311, 340
Cfr42I CCGCGG 1 cut(s) 888
CspCI CAANNNNNGTGG 2 cut(s) 918, 953
CviAII CATG 7 cut(s) 37, 62, 446, 604, 627, 873, 878
DdeI CTNAG 2 cut(s) 656, 749
DpnI GATC 4 cut(s) 435, 516, 747, 927
DpnII GATC 4 cut(s) 433, 514, 745, 925
DraI TTTAAA 1 cut(s) 813
DrdI GACNNNNNNGTC 1 cut(s) 338
DriI GACNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 338
Eam1104I CTCTTC 2 cut(s) 222, 378
Eam1105I GACNNNNNGTC 1 cut(s) 92
EarI CTCTTC 2 cut(s) 222, 378
Eco47I GGWCC 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 293
EcoO109I RGGNCCY 2 cut(s) 311, 340
EcoRII CCWGG 1 cut(s) 118
EcoT22I ATGCAT 1 cut(s) 63
FaeI CATG 7 cut(s) 40, 65, 449, 607, 630, 876, 881
FatI CATG 7 cut(s) 36, 61, 445, 603, 626, 872, 877
FauNDI CATATG 1 cut(s) 258
FbaI TGATCA 1 cut(s) 433
Fnu4HI GCNGC 4 cut(s) 526, 682, 981, 984
Fsp4HI GCNGC 4 cut(s) 526, 682, 981, 984
FspBI CTAG 1 cut(s) 689
GluI GCNGC 4 cut(s) 526, 682, 981, 984
HaeIII GGCC 1 cut(s) 312
HapII CCGG 2 cut(s) 392, 662
Hin1II CATG 7 cut(s) 40, 65, 449, 607, 630, 876, 881
HincII GTYRAC 2 cut(s) 33, 757
HindII GTYRAC 2 cut(s) 33, 757
HinfI GANTC 7 cut(s) 104, 241, 286, 472, 497, 552, 658
HpaI GTTAAC 2 cut(s) 33, 757
HpaII CCGG 2 cut(s) 392, 662
HphI GGTGA 2 cut(s) 641, 704
Hpy166II GTNNAC 2 cut(s) 33, 757
Hpy188I TCNGA 5 cut(s) 197, 268, 438, 580, 616
Hpy188III TCNNGA 6 cut(s) 146, 283, 326, 503, 707, 923
Hpy8I GTNNAC 2 cut(s) 33, 757
HpyAV CCTTC 5 cut(s) 262, 689, 782, 863, 972
HpyCH4III ACNGT 3 cut(s) 50, 131, 896
HpyCH4V TGCA 8 cut(s) 61, 418, 562, 607, 684, 779, 944, 983
HpyF10VI GCNNNNNNNGC 2 cut(s) 415, 799
HpyF3I CTNAG 2 cut(s) 656, 749
Hsp92II CATG 7 cut(s) 40, 65, 449, 607, 630, 876, 881
Ksp22I TGATCA 1 cut(s) 433
KspAI GTTAAC 2 cut(s) 33, 757
KspI CCGCGG 1 cut(s) 888
Kzo9I GATC 4 cut(s) 433, 514, 745, 925
LmnI GCTCC 1 cut(s) 698
Lsp1109I GCAGC 3 cut(s) 512, 668, 967
LweI GCATC 1 cut(s) 427
MaeI CTAG 1 cut(s) 689
MaeIII GTNAC 2 cut(s) 146, 880
MalI GATC 4 cut(s) 435, 516, 747, 927
MboI GATC 4 cut(s) 433, 514, 745, 925
MboII GAAGA 7 cut(s) 96, 239, 314, 365, 462, 467, 749
MfeI CAATTG 2 cut(s) 842, 975
MflI RGATCY 2 cut(s) 745, 925
MlyI GAGTC 3 cut(s) 113, 481, 667
MnlI CCTC 2 cut(s) 353, 739
Mph1103I ATGCAT 1 cut(s) 63
MseI TTAA 8 cut(s) 5, 32, 468, 668, 756, 812, 837, 911
MspA1I CMGCKG 1 cut(s) 887
MspI CCGG 2 cut(s) 392, 662
MspR9I CCNGG 2 cut(s) 120, 663
MssI GTTTAAAC 1 cut(s) 813
MunI CAATTG 2 cut(s) 842, 975
MvaI CCWGG 1 cut(s) 120
MvnI CGCG 1 cut(s) 887
MwoI GCNNNNNNNGC 2 cut(s) 415, 799
NciI CCSGG 1 cut(s) 663
NdeI CATATG 1 cut(s) 258
NdeII GATC 4 cut(s) 433, 514, 745, 925
NlaIII CATG 7 cut(s) 40, 65, 449, 607, 630, 876, 881
NlaIV GGNNCC 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 146
NsiI ATGCAT 1 cut(s) 63
NspI RCATGY 2 cut(s) 607, 876
PaeI GCATGC 1 cut(s) 607
PciI ACATGT 1 cut(s) 872
PfeI GAWTC 4 cut(s) 241, 286, 497, 552
PfoI TCCNGGA 1 cut(s) 661
PkrI GCNGC 4 cut(s) 527, 683, 982, 985
PleI GAGTC 3 cut(s) 112, 480, 666
PmeI GTTTAAAC 1 cut(s) 813
PpsI GAGTC 3 cut(s) 112, 480, 666
PpuMI RGGWCCY 1 cut(s) 340
PscI ACATGT 1 cut(s) 872
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 1 cut(s) 341
PspPI GGNCC 2 cut(s) 311, 340
PspPPI RGGWCCY 1 cut(s) 340
PstI CTGCAG 1 cut(s) 985
PsuI RGATCY 2 cut(s) 745, 925
SacII CCGCGG 1 cut(s) 888
SaqAI TTAA 8 cut(s) 5, 32, 468, 668, 756, 812, 837, 911
SatI GCNGC 4 cut(s) 526, 682, 981, 984
Sau3AI GATC 4 cut(s) 433, 514, 745, 925
Sau96I GGNCC 2 cut(s) 311, 340
SchI GAGTC 3 cut(s) 113, 481, 667
ScrFI CCNGG 2 cut(s) 120, 663
SetI ASST 5 cut(s) 238, 411, 643, 694, 804
SfaNI GCATC 1 cut(s) 427
SfcI CTRYAG 1 cut(s) 981
Sfr303I CCGCGG 1 cut(s) 888
SgrBI CCGCGG 1 cut(s) 888
SinI GGWCC 1 cut(s) 340
SmlI CTYRAG 2 cut(s) 281, 803
SmoI CTYRAG 2 cut(s) 281, 803
SphI GCATGC 1 cut(s) 607
SsiI CCGC 2 cut(s) 885, 887
SspMI CTAG 1 cut(s) 689
StyD4I CCNGG 2 cut(s) 118, 661
TaaI ACNGT 3 cut(s) 50, 131, 896
TaqI TCGA 2 cut(s) 345, 797
TfiI GAWTC 4 cut(s) 241, 286, 497, 552
Tru1I TTAA 8 cut(s) 5, 32, 468, 668, 756, 812, 837, 911
Tru9I TTAA 8 cut(s) 5, 32, 468, 668, 756, 812, 837, 911
TscAI CASTG 1 cut(s) 660
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 4 cut(s) 525, 681, 980, 983
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 581
TspGWI ACGGA 2 cut(s) 86, 721
TspRI CASTG 1 cut(s) 660
VpaK11BI GGWCC 1 cut(s) 340
XapI RAATTY 2 cut(s) 292, 352
XceI RCATGY 2 cut(s) 607, 876
XspI CTAG 1 cut(s) 689
Zsp2I ATGCAT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.