Rorug06G0292900

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001271
Physical Location & Seq
Reverse (-)
44478451 .. 44483535
5085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0292900.1

Sequence Viewer

Length: 1350 bp
ATGGCGAGTTCGTATCCTGGACCTGTTAGTGCTGTTCAGGTAGGGTCGTACTTTGTGGGACAGTACTACCAGGTTCTTCAGCAGCAACCCGATCTTGTTCATCAGTTTTATTCTGATGGCAGCACCATGATTCGTGTCGATGGCGATTCGACAGAATCTGCTAATGAAATGCTGCAAATTCATACGCTCATATTGTCGCAGTATTTTACTTCGATTGAGATCAAGACTATCAATTCCATCAATTCCTTGGGTGGAGGTGTTCTTGTGATGGTTACCGGTTCTGTTAAGTCCAAGGAATTCAATGGGAGGAGGAAGTTTGTCCAGACCTTCTTTCTGGCTCCTCAGAATAAAGGATACTATGTTCTCAATGATATCTTCCAGTTTCTCGAGGATGAGGTAGTTTTACAGCATCCTGCAAACATGATATCGGAGGGCCAATTCGATATTCAACTAAATGCTTCTAGCCCTCATCGAGAACCGCCAGTTTCCGACTATGTTCTGGAGGAAGAGGCCAGGGAGTATGTGAACTCTGTCGACATAGAAGACGATCCAGTTGATAAGTATAGTCTGCCAGAGCAACAGGTACAGCAAGAATTTGAGACTGAAGTGGTGGTGGAGGAAACTCCTCAAGAGGAGACCTATGCCTCATTTCAAAGTGCGGTGAACACTGTGCAAGACACTCCTGTTGTTTCTGTGGAGGAACCAGTGGGAGAACCTCAGAAGAAGACTTGGGCTTCTATATTGCGAGTTGCTAAGGGACCGTCAGCACCAGAAGTAACTCAACAACCCTCTTCTAGAAGTGTTCAAACTGCTCCAGATTGGAATCACATCCCTCAGCCCCCTGTTGAACAATCAAACTCTGCTTCAGACTGGAGTTACACACCTCAGCCCACTGTAGAAGCAGCAGAGGAGAGTTACATGCCAGAGGAAGAAGGTGAAATGAAATCGGTGTACGTAAGAAACTTACCTCCTACAGTTACCGAAGGTGAAATCGAGGAAGAGTTTAAGCACTTTGGTCAAATAATACCTGATGGCGTCTTTGTTAGGGCTCGCAAGGAAATTGGAGTCTGCTATGCTTTTGTTGAATTTGAAGACATGGTTGGTGTTCACAATGCCCTCAAGGCATCTCCAATTCATTTGGCTGGAAGACAAGTGTACATTGAGGAGCGAAGACCGAACAGCACCGGTGCTGCTCGAGGAAGAGGAAGGGGGAGAGGCAGAAGCAGTTACCCAATTGACGCCACAAGGGGGCGTTTTGGAGGTCGTAGTTCGGGCAGAGGTAGTTACCAGGATAGTGGCGACTACAATAGAGGGAGAGGCAATGGCTTTTATCAGCGTGGTACACGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

449

Amino Acids

50.11

Weight (kDa)

4.79

Isoelectric Point (pI)

61.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTF2 PF02136 14 - 128 8.5e-28 Nuclear transport factor 2 (NTF2) domain
RRM_1 PF00076 317 - 375 9.9e-09 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 534
AciI CCGC 2 cut(s) 479, 659
AclWI GGATC 1 cut(s) 542
AcsI RAATTY 4 cut(s) 177, 296, 593, 1085
AcuI CTGAAG 3 cut(s) 62, 624, 849
AcyI GRCGYC 2 cut(s) 1035, 1239
AfaI GTAC 6 cut(s) 50, 65, 585, 953, 1157, 1342
AfiI CCNNNNNNNGG 1 cut(s) 1248
AgeI ACCGGT 2 cut(s) 275, 1184
AgsI TTSAA 7 cut(s) 301, 449, 653, 806, 848, 1085, 1091
AjnI CCWGG 4 cut(s) 16, 69, 512, 1287
AjuI GAANNNNNNNTTGG 4 cut(s) 429, 461, 1083, 1115
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
Alw26I GTCTC 2 cut(s) 593, 629
AlwI GGATC 1 cut(s) 542
AlwNI CAGNNNCTG 1 cut(s) 158
Ama87I CYCGRG 2 cut(s) 386, 1194
AoxI GGCC 2 cut(s) 433, 510
ApeKI GCWGC 5 cut(s) 82, 120, 172, 902, 1190
ApoI RAATTY 4 cut(s) 177, 296, 593, 1085
AsiGI ACCGGT 2 cut(s) 275, 1184
AspS9I GGNCC 3 cut(s) 20, 433, 758
AsuHPI GGTGA 3 cut(s) 673, 947, 998
AvaI CYCGRG 2 cut(s) 386, 1194
AvaII GGWCC 2 cut(s) 20, 758
BanII GRGCYC 1 cut(s) 1051
BbsI GAAGAC 5 cut(s) 549, 731, 1098, 1153, 1177
BbvCI CCTCAGC 2 cut(s) 834, 885
BbvI GCAGC 5 cut(s) 94, 132, 159, 914, 1177
BccI CCATC 5 cut(s) 110, 134, 245, 262, 1025
BciT130I CCWGG 4 cut(s) 18, 71, 514, 1289
BciVI GTATCC 2 cut(s) 24, 347
BcoDI GTCTC 2 cut(s) 593, 629
BfaI CTAG 2 cut(s) 462, 795
BfmI CTRYAG 2 cut(s) 894, 972
BfuI GTATCC 2 cut(s) 24, 347
BglI GCCNNNNNGGC 1 cut(s) 1121
BisI GCNGC 5 cut(s) 83, 121, 173, 903, 1191
BlsI GCNGC 5 cut(s) 84, 122, 174, 904, 1192
BmcAI AGTACT 1 cut(s) 65
Bme1390I CCNGG 4 cut(s) 18, 71, 514, 1289
Bme18I GGWCC 2 cut(s) 20, 758
BmeT110I CYCGRG 2 cut(s) 386, 1194
BmgT120I GGNCC 3 cut(s) 20, 433, 758
BmiI GGNNCC 3 cut(s) 339, 702, 759
BmrFI CCNGG 4 cut(s) 18, 71, 514, 1289
BmsI GCATC 2 cut(s) 418, 1133
BpiI GAAGAC 5 cut(s) 549, 731, 1098, 1153, 1177
BpmI CTGGAG 3 cut(s) 521, 798, 892
Bpu10I CCTNAGC 3 cut(s) 753, 834, 885
BpuEI CTTGAG 2 cut(s) 612, 1103
BsaAI YACGTR 1 cut(s) 955
BsaBI GATNNNNATC 2 cut(s) 218, 822
BsaHI GRCGYC 2 cut(s) 1035, 1239
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 3 cut(s) 246, 291, 513
BsaWI WCCGGW 2 cut(s) 275, 1184
Bsc4I CCNNNNNNNGG 1 cut(s) 1248
Bse118I RCCGGY 2 cut(s) 275, 1184
Bse1I ACTGG 5 cut(s) 379, 482, 551, 704, 875
Bse3DI GCAATG 1 cut(s) 1327
Bse8I GATNNNNATC 2 cut(s) 218, 822
BseBI CCWGG 4 cut(s) 18, 71, 514, 1289
BseDI CCNNGG 3 cut(s) 246, 291, 513
BseGI GGATG 3 cut(s) 397, 409, 828
BseJI GATNNNNATC 2 cut(s) 218, 822
BseLI CCNNNNNNNGG 1 cut(s) 1248
BseMI GCAATG 1 cut(s) 1327
BseMII CTCAG 4 cut(s) 356, 731, 848, 899
BseNI ACTGG 5 cut(s) 379, 482, 551, 704, 875
BseRI GAGGAG 6 cut(s) 322, 330, 615, 647, 923, 1178
BseXI GCAGC 5 cut(s) 94, 132, 159, 914, 1177
BshFI GGCC 2 cut(s) 435, 512
BshTI ACCGGT 2 cut(s) 275, 1184
BsiHKCI CYCGRG 2 cut(s) 386, 1194
BsiSI CCGG 2 cut(s) 276, 1185
BslFI GGGAC 2 cut(s) 72, 771
BslI CCNNNNNNNGG 1 cut(s) 1248
BsmAI GTCTC 2 cut(s) 593, 629
BsmFI GGGAC 2 cut(s) 72, 771
BsnI GGCC 2 cut(s) 435, 512
Bso31I GGTCTC 1 cut(s) 629
BsoBI CYCGRG 2 cut(s) 386, 1194
Bsp1286I GDGCHC 1 cut(s) 1051
Bsp1407I TGTACA 1 cut(s) 1155
Bsp143I GATC 3 cut(s) 91, 219, 547
BspACI CCGC 2 cut(s) 479, 659
BspANI GGCC 2 cut(s) 435, 512
BspCNI CTCAG 4 cut(s) 355, 730, 847, 898
BspLI GGNNCC 3 cut(s) 339, 702, 759
BspPI GGATC 1 cut(s) 542
BspTNI GGTCTC 1 cut(s) 629
BsrDI GCAATG 1 cut(s) 1327
BsrFI RCCGGY 2 cut(s) 275, 1184
BsrGI TGTACA 1 cut(s) 1155
BsrI ACTGG 5 cut(s) 379, 482, 551, 704, 875
BssAI RCCGGY 2 cut(s) 275, 1184
BssECI CCNNGG 3 cut(s) 246, 291, 513
BssMI GATC 3 cut(s) 91, 219, 547
BssNI GRCGYC 2 cut(s) 1035, 1239
BssT1I CCWWGG 2 cut(s) 246, 291
Bst2UI CCWGG 4 cut(s) 18, 71, 514, 1289
Bst4CI ACNGT 5 cut(s) 63, 670, 762, 895, 976
Bst6I CTCTTC 4 cut(s) 501, 796, 993, 1195
BstACI GRCGYC 2 cut(s) 1035, 1239
BstAUI TGTACA 1 cut(s) 1155
BstBAI YACGTR 1 cut(s) 955
BstC8I GCNNGC 1 cut(s) 1051
BstDEI CTNAG 5 cut(s) 342, 717, 753, 834, 885
BstEII GGTNACC 1 cut(s) 271
BstF5I GGATG 3 cut(s) 397, 409, 828
BstKTI GATC 3 cut(s) 94, 222, 550
BstMAI GTCTC 2 cut(s) 593, 629
BstMBI GATC 3 cut(s) 91, 219, 547
BstMWI GCNNNNNNNGC 1 cut(s) 1121
BstNI CCWGG 4 cut(s) 18, 71, 514, 1289
BstNSI RCATGY 1 cut(s) 922
BstPI GGTNACC 1 cut(s) 271
BstSCI CCNGG 4 cut(s) 16, 69, 512, 1287
BstSFI CTRYAG 2 cut(s) 894, 972
BstSNI TACGTA 1 cut(s) 955
BstV1I GCAGC 5 cut(s) 94, 132, 159, 914, 1177
BstV2I GAAGAC 5 cut(s) 549, 731, 1098, 1153, 1177
BstXI CCANNNNNNTGG 1 cut(s) 1295
BsuI GTATCC 2 cut(s) 24, 347
BsuRI GGCC 2 cut(s) 435, 512
BtsCI GGATG 3 cut(s) 397, 409, 828
BtsIMutI CAGTG 3 cut(s) 666, 711, 891
Cac8I GCNNGC 1 cut(s) 1051
CaiI CAGNNNCTG 1 cut(s) 158
Cfr10I RCCGGY 2 cut(s) 275, 1184
Cfr13I GGNCC 3 cut(s) 20, 433, 758
CseI GACGC 2 cut(s) 1024, 1247
CsiI ACCWGGT 1 cut(s) 69
Csp6I GTAC 6 cut(s) 49, 64, 584, 952, 1156, 1341
CspAI ACCGGT 2 cut(s) 275, 1184
CviAII CATG 4 cut(s) 127, 421, 919, 1096
CviQI GTAC 6 cut(s) 49, 64, 584, 952, 1156, 1341
DdeI CTNAG 5 cut(s) 342, 717, 753, 834, 885
DpnI GATC 3 cut(s) 93, 221, 549
DpnII GATC 3 cut(s) 91, 219, 547
Eam1104I CTCTTC 4 cut(s) 501, 796, 993, 1195
EarI CTCTTC 4 cut(s) 501, 796, 993, 1195
Eco105I TACGTA 1 cut(s) 955
Eco130I CCWWGG 2 cut(s) 246, 291
Eco24I GRGCYC 1 cut(s) 1051
Eco31I GGTCTC 1 cut(s) 629
Eco32I GATATC 2 cut(s) 373, 426
Eco47I GGWCC 2 cut(s) 20, 758
Eco57I CTGAAG 3 cut(s) 62, 624, 849
Eco88I CYCGRG 2 cut(s) 386, 1194
Eco91I GGTNACC 1 cut(s) 271
EcoO65I GGTNACC 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 296
EcoRII CCWGG 4 cut(s) 16, 69, 512, 1287
EcoRV GATATC 2 cut(s) 373, 426
EcoT14I CCWWGG 2 cut(s) 246, 291
EcoT38I GRGCYC 1 cut(s) 1051
ErhI CCWWGG 2 cut(s) 246, 291
FaeI CATG 4 cut(s) 130, 424, 922, 1099
FaqI GGGAC 2 cut(s) 72, 771
FatI CATG 4 cut(s) 126, 420, 918, 1095
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 5 cut(s) 83, 121, 173, 903, 1191
FokI GGATG 3 cut(s) 396, 404, 815
FriOI GRGCYC 1 cut(s) 1051
Fsp4HI GCNGC 5 cut(s) 83, 121, 173, 903, 1191
FspBI CTAG 2 cut(s) 462, 795
GluI GCNGC 5 cut(s) 83, 121, 173, 903, 1191
GsuI CTGGAG 3 cut(s) 521, 798, 892
HaeIII GGCC 2 cut(s) 435, 512
HapII CCGG 2 cut(s) 276, 1185
HgaI GACGC 2 cut(s) 1024, 1247
Hin1I GRCGYC 2 cut(s) 1035, 1239
Hin1II CATG 4 cut(s) 130, 424, 922, 1099
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 5 cut(s) 130, 146, 155, 823, 1065
HpaII CCGG 2 cut(s) 276, 1185
HphI GGTGA 3 cut(s) 673, 947, 998
Hpy166II GTNNAC 7 cut(s) 526, 535, 664, 952, 1108, 1156, 1343
Hpy188I TCNGA 6 cut(s) 115, 345, 430, 490, 720, 868
Hpy188III TCNNGA 8 cut(s) 223, 322, 386, 473, 500, 629, 795, 815
Hpy8I GTNNAC 7 cut(s) 526, 535, 664, 952, 1108, 1156, 1343
HpyAV CCTTC 4 cut(s) 337, 926, 977, 1200
HpyCH4III ACNGT 5 cut(s) 63, 670, 762, 895, 976
HpyCH4IV ACGT 1 cut(s) 954
HpyCH4V TGCA 3 cut(s) 175, 416, 673
HpyF10VI GCNNNNNNNGC 1 cut(s) 1121
HpyF3I CTNAG 5 cut(s) 342, 717, 753, 834, 885
HpySE526I ACGT 1 cut(s) 954
Hsp92I GRCGYC 2 cut(s) 1035, 1239
Hsp92II CATG 4 cut(s) 130, 424, 922, 1099
Kzo9I GATC 3 cut(s) 91, 219, 547
LmnI GCTCC 3 cut(s) 343, 817, 1165
Lsp1109I GCAGC 5 cut(s) 94, 132, 159, 914, 1177
LweI GCATC 2 cut(s) 418, 1133
MabI ACCWGGT 1 cut(s) 69
MaeI CTAG 2 cut(s) 462, 795
MaeII ACGT 1 cut(s) 954
MaeIII GTNAC 7 cut(s) 271, 775, 875, 914, 976, 1226, 1283
MalI GATC 3 cut(s) 93, 221, 549
MboI GATC 3 cut(s) 91, 219, 547
MfeI CAATTG 1 cut(s) 1233
MhlI GDGCHC 1 cut(s) 1051
MlyI GAGTC 1 cut(s) 1074
MmeI TCCRAC 1 cut(s) 513
MseI TTAA 2 cut(s) 285, 1005
MspI CCGG 2 cut(s) 276, 1185
MspR9I CCNGG 4 cut(s) 18, 71, 514, 1289
MunI CAATTG 1 cut(s) 1233
MvaI CCWGG 4 cut(s) 18, 71, 514, 1289
MwoI GCNNNNNNNGC 1 cut(s) 1121
NdeII GATC 3 cut(s) 91, 219, 547
NlaIII CATG 4 cut(s) 130, 424, 922, 1099
NlaIV GGNNCC 3 cut(s) 339, 702, 759
NspI RCATGY 1 cut(s) 922
PaeR7I CTCGAG 2 cut(s) 386, 1194
PfeI GAWTC 4 cut(s) 130, 146, 155, 823
PfoI TCCNGGA 1 cut(s) 16
PinAI ACCGGT 2 cut(s) 275, 1184
PkrI GCNGC 5 cut(s) 84, 122, 174, 904, 1192
PleI GAGTC 1 cut(s) 1073
PpsI GAGTC 1 cut(s) 1073
Ppu21I YACGTR 1 cut(s) 955
Psp6I CCWGG 4 cut(s) 16, 69, 512, 1287
PspEI GGTNACC 1 cut(s) 271
PspGI CCWGG 4 cut(s) 16, 69, 512, 1287
PspN4I GGNNCC 3 cut(s) 339, 702, 759
PspPI GGNCC 3 cut(s) 20, 433, 758
PspXI VCTCGAGB 1 cut(s) 1194
PstNI CAGNNNCTG 1 cut(s) 158
RsaI GTAC 6 cut(s) 50, 65, 585, 953, 1157, 1342
RsaNI GTAC 6 cut(s) 49, 64, 584, 952, 1156, 1341
SalI GTCGAC 1 cut(s) 533
SaqAI TTAA 2 cut(s) 285, 1005
SatI GCNGC 5 cut(s) 83, 121, 173, 903, 1191
Sau3AI GATC 3 cut(s) 91, 219, 547
Sau96I GGNCC 3 cut(s) 20, 433, 758
ScaI AGTACT 1 cut(s) 65
SchI GAGTC 1 cut(s) 1074
ScrFI CCNGG 4 cut(s) 18, 71, 514, 1289
SduI GDGCHC 1 cut(s) 1051
SexAI ACCWGGT 1 cut(s) 69
SfaNI GCATC 2 cut(s) 418, 1133
SfcI CTRYAG 2 cut(s) 894, 972
Sfr274I CTCGAG 2 cut(s) 386, 1194
SgrAI CRCCGGYG 1 cut(s) 1184
SinI GGWCC 2 cut(s) 20, 758
SlaI CTCGAG 2 cut(s) 386, 1194
SmlI CTYRAG 4 cut(s) 386, 627, 1118, 1194
SmoI CTYRAG 4 cut(s) 386, 627, 1118, 1194
SnaBI TACGTA 1 cut(s) 955
SsiI CCGC 2 cut(s) 479, 659
SspMI CTAG 2 cut(s) 462, 795
StyD4I CCNGG 4 cut(s) 16, 69, 512, 1287
StyI CCWWGG 2 cut(s) 246, 291
TaaI ACNGT 5 cut(s) 63, 670, 762, 895, 976
TaiI ACGT 1 cut(s) 957
TaqI TCGA 9 cut(s) 138, 149, 212, 387, 441, 472, 534, 993, 1195
TaqII GACCGA 1 cut(s) 1189
TatI WGTACW 2 cut(s) 63, 1155
TfiI GAWTC 4 cut(s) 130, 146, 155, 823
Tru1I TTAA 2 cut(s) 285, 1005
Tru9I TTAA 2 cut(s) 285, 1005
TscAI CASTG 3 cut(s) 673, 711, 898
TseI GCWGC 5 cut(s) 82, 120, 172, 902, 1190
TspDTI ATGAA 5 cut(s) 89, 170, 180, 956, 1124
TspRI CASTG 3 cut(s) 673, 711, 898
VpaK11BI GGWCC 2 cut(s) 20, 758
XapI RAATTY 4 cut(s) 177, 296, 593, 1085
XbaI TCTAGA 1 cut(s) 794
XceI RCATGY 1 cut(s) 922
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XhoI CTCGAG 2 cut(s) 386, 1194
XmiI GTMKAC 1 cut(s) 534
XspI CTAG 2 cut(s) 462, 795
ZrmI AGTACT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.