Prupe.1G389800_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0001271
Physical Location & Seq
Forward (+)
34798414 .. 34803563
5150 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G389800.1

Sequence Viewer

Length: 2103 bp
ATGCCTTCTTCTTCCTTTTCTCTTCGCAGACCCTCTGCCTCCACCTCTACCTCTACCTCTACCTCCTCTGTTTCTTCTCCTTCTGCTTCCTCTGTTTCAGCTCTGAAACAGGACACTGGGGGCAGCTGTGAGCAGAGCCCCAGGCGGCTCACGCGGCAGAAGAAGCTCCGCCACATCAATGATCAGGACCTGTGTTTCCCCCTCTCTGAAAAGTCTCGCTCTTCGCCCGCTTCGCCTGAATATTCCACCCGAAAGTCAACGTCTCCGGATCGTGAGTCCAAGTCAAACCACTGGTCCTCTTCGGCTGCTGCTGCTCCACAACCCTTGCCTCTACCGGAATCTCCCGTGATCGGGAGACCCGGCTCTGTGCCCAAAGATGGGTACTTCGGCCATGCGTTCTTCAGGAAAAAAAGAAATCAAGTTGCCACAACCTCAGCACCACAGATTTCTAATTCAATCAATAATCAAGGGGCATTCTCCTCAGCACCACAGAATTGTAATTCAAGCAATCGTCAAGGGCCATCCTCCTCTGCACCACTGACTAATTCAAGCAATCGTCGAAGGGCATTATCTTCCCAAGATGTAAATAATAATGGTGGGGGTTTTAATTACAACTTGGGACTGAATGTTTTTTCTAGGACAGCTCAGGCAAATGGTGTCTCAAGTCATCCTCTTAGCCCACAAAGATCAAACTTTGTGGAGATGGATGGTTTTCCTGTACATGTGGCCACCACTTCATCCATTAATTCTTCAAGCAATTATCATAGGGGAAAGAGACAAGAAAATGAAAGTTACAACTTCAGCAAGAGTGCTCCAACAAGTGGGTTTTCCAGTCCTGCAGTTAGCCCTCGAAGATCAAAGGCGGGGGATCCTTTTCCTGCGTTTGGGGCTTCTCAAGAACAATATCAGGATACCTGTCCTTCAAGGTTATCACCAAACAATATTGCTCATAGTCCCAGTCATTCTCCTCTTCCCAGTCCCACATCTCGAAGTCCACGCTTCAACCACAAAAATTCAAATGGAAATGTATTTGCGTTGCATCGTAAATCATTCACAGAGGGTTTTTCAGAGAGGGGTGAAAGTAGTAGTCAAGCCAATGCTCACCCTTTGCCTTTGCCTCCTGGAGCCGTGGTACTTCCACAGCCATCTAGCATGCATCACAATGCAGAACTTCTGTCCACATCATCAATGAATGGTCAATGGCAGAAGGGAAAACTTATTGGACGAGGTACCTTTGGTAGTGTTTATCTTGCAACCAACCGAGAGACCGGGGCCTTGTGTGCAATGAAGGAAGTTGATCTCATTCCCGATGATCCTAAATCTGCTGAATGTATAAAGCAATTGGAGCAGGAAATTAAAGTCCTCCGTGCACTAAAGCATCCAAATATTGTGCAGTATTATGGTAGTGAAGTGATTGATGATCACTTCTACATATACTTGGAGTATGTTCATCCTGGATCAATTAATAAGTACGTCCAAGACCATATTGGAGCTATGACAGAATCTGTAGTTCGCAATTTTACTCGCCATATCCTCTCTGGGTTGGCTTTTTTGCATAGCAAAAAGACAATTCACAGGGATATAAAGGGTGCAAATTTGCTTGTTGATGCATCAGGCGTTGTTAAACTTGCTGATTTTGGGATGGCAAAACATCTTAACGGACACTCATACAATCTTTCTCTGAAGGGCAGTCCATACTGGATAGCTCCTGAGGTCATAAAGGCTGTGATGCAGAATAATACTGATCCTGATCTTGCTTTGGCTGTTGATATATGGAGCTTGGGTTGCACAATCGTTGAGATGTTCAATGGAAAACCTCCTTGGAGTGACTTTACAGGACCTCAAGCTATGTTCAAGGTTCTGAACACAATCCCAGATATACCAGAAACATTATCTGCAGAGGGAAAGGACTTCCTCAGTTGGTGTTTCCGTAGGAACCCTGCCGAGAGACCATCAGCTAATCAGCTACTAGAGCATCCTTTTGTACGAAATCCACATGATCAAACTGTCTCACACTATGCCCAGAATTTTGCTCTGATGAACCTAATCGATAAATTGCATAGTCCGACGGACCATACTAAGCCAAAAAATGGGCGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

701

Amino Acids

76.4

Weight (kDa)

9.45

Isoelectric Point (pI)

58.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1229
AccB1I GGYRCC 1 cut(s) 1229
AccB7I CCANNNNNTGG 2 cut(s) 821, 2090
AccII CGCG 1 cut(s) 154
AccIII TCCGGA 1 cut(s) 265
AciI CCGC 5 cut(s) 145, 154, 169, 228, 863
AclWI GGATC 6 cut(s) 276, 863, 876, 1307, 1465, 1739
AcoI YGGCCR 2 cut(s) 388, 726
AcsI RAATTY 3 cut(s) 1012, 1594, 2026
AcuI CTGAAG 3 cut(s) 385, 784, 1703
AfaI GTAC 6 cut(s) 383, 720, 1134, 1231, 1472, 1986
AfiI CCNNNNNNNGG 7 cut(s) 350, 351, 377, 378, 821, 884, 2090
AflIII ACRYGT 1 cut(s) 721
AgsI TTSAA 9 cut(s) 456, 504, 549, 753, 924, 1003, 1017, 1807, 1855
AjnI CCWGG 3 cut(s) 140, 1120, 1453
AjuI GAANNNNNNNTTGG 2 cut(s) 1804, 1836
Alw21I GWGCWC 2 cut(s) 814, 1372
Alw26I GTCTC 8 cut(s) 219, 267, 349, 664, 769, 1259, 1942, 2014
Alw44I GTGCAC 1 cut(s) 1368
AlwI GGATC 6 cut(s) 276, 863, 876, 1307, 1465, 1739
AlwNI CAGNNNCTG 2 cut(s) 190, 1505
Aor13HI TCCGGA 1 cut(s) 265
AoxI GGCC 4 cut(s) 388, 518, 726, 1272
ApaLI GTGCAC 1 cut(s) 1368
ApeKI GCWGC 4 cut(s) 123, 305, 308, 311
ApoI RAATTY 3 cut(s) 1012, 1594, 2026
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
AseI ATTAAT 2 cut(s) 744, 1464
Asp718I GGTACC 1 cut(s) 1229
AspS9I GGNCC 6 cut(s) 187, 294, 518, 1272, 1838, 2071
AsuC2I CCSGG 2 cut(s) 360, 1270
AsuHPI GGTGA 3 cut(s) 924, 1088, 1094
AvaII GGWCC 4 cut(s) 187, 294, 1838, 2071
AxyI CCTNAGG 1 cut(s) 1710
BaeGI GKGCMC 2 cut(s) 372, 1372
BalI TGGCCA 1 cut(s) 728
BamHI GGATCC 1 cut(s) 868
BanI GGYRCC 1 cut(s) 1229
BanII GRGCYC 1 cut(s) 140
Bbv12I GWGCWC 2 cut(s) 814, 1372
BbvCI CCTCAGC 2 cut(s) 433, 481
BbvI GCAGC 4 cut(s) 135, 292, 295, 298
BccI CCATC 7 cut(s) 371, 529, 697, 701, 1153, 1636, 1960
BceAI ACGGC 1 cut(s) 1112
BciT130I CCWGG 3 cut(s) 142, 1122, 1455
BciVI GTATCC 1 cut(s) 904
BclI TGATCA 3 cut(s) 181, 1420, 1999
BcnI CCSGG 2 cut(s) 360, 1270
BcoDI GTCTC 8 cut(s) 219, 267, 349, 664, 769, 1259, 1942, 2014
BfaI CTAG 3 cut(s) 636, 1149, 1970
BfmI CTRYAG 3 cut(s) 837, 1506, 1896
BfuI GTATCC 1 cut(s) 904
BisI GCNGC 6 cut(s) 124, 146, 155, 306, 309, 312
BlsI GCNGC 6 cut(s) 125, 147, 156, 307, 310, 313
Bme1390I CCNGG 5 cut(s) 142, 360, 1122, 1270, 1455
Bme18I GGWCC 4 cut(s) 187, 294, 1838, 2071
BmgT120I GGNCC 6 cut(s) 187, 294, 518, 1272, 1838, 2071
BmiI GGNNCC 5 cut(s) 870, 1126, 1231, 1273, 1937
BmrFI CCNGG 5 cut(s) 142, 360, 1122, 1270, 1455
BmrI ACTGGG 3 cut(s) 126, 951, 969
BmsI GCATC 7 cut(s) 1048, 1165, 1387, 1597, 1619, 1719, 1984
BmuI ACTGGG 3 cut(s) 126, 951, 969
BpmI CTGGAG 1 cut(s) 1143
Bpu10I CCTNAGC 3 cut(s) 433, 481, 645
BpuEI CTTGAG 3 cut(s) 646, 879, 1827
BpuMI CCSGG 2 cut(s) 360, 1270
Bsa29I ATCGAT 1 cut(s) 2049
BsaBI GATNNNNATC 1 cut(s) 1749
BsaI GGTCTC 3 cut(s) 349, 1259, 1942
BsaJI CCNNGG 4 cut(s) 140, 1128, 1269, 1820
BsaWI WCCGGW 2 cut(s) 265, 334
BsaXI ACNNNNNCTCC 4 cut(s) 1116, 1146, 1768, 1798
Bsc4I CCNNNNNNNGG 7 cut(s) 350, 351, 377, 378, 821, 884, 2090
Bse1I ACTGG 6 cut(s) 121, 296, 831, 957, 975, 1703
Bse21I CCTNAGG 1 cut(s) 1710
Bse3DI GCAATG 1 cut(s) 1290
Bse8I GATNNNNATC 1 cut(s) 1749
BseAI TCCGGA 1 cut(s) 265
BseBI CCWGG 3 cut(s) 142, 1122, 1455
BseCI ATCGAT 1 cut(s) 2049
BseDI CCNNGG 4 cut(s) 140, 1128, 1269, 1820
BseGI GGATG 8 cut(s) 521, 667, 712, 737, 1378, 1450, 1647, 1975
BseJI GATNNNNATC 1 cut(s) 1749
BseLI CCNNNNNNNGG 7 cut(s) 350, 351, 377, 378, 821, 884, 2090
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 5 cut(s) 447, 495, 659, 1701, 1930
BseNI ACTGG 6 cut(s) 121, 296, 831, 957, 975, 1703
BseRI GAGGAG 4 cut(s) 55, 469, 517, 957
BseSI GKGCMC 2 cut(s) 372, 1372
BseXI GCAGC 4 cut(s) 135, 292, 295, 298
BsgI GTGCAG 2 cut(s) 516, 1412
Bsh1236I CGCG 1 cut(s) 154
BshFI GGCC 4 cut(s) 390, 520, 728, 1274
BshNI GGYRCC 1 cut(s) 1229
BshVI ATCGAT 1 cut(s) 2049
BsiHKAI GWGCWC 2 cut(s) 814, 1372
BsiSI CCGG 4 cut(s) 266, 335, 360, 1269
BslFI GGGAC 3 cut(s) 633, 939, 963
BslI CCNNNNNNNGG 7 cut(s) 350, 351, 377, 378, 821, 884, 2090
BsmAI GTCTC 8 cut(s) 219, 267, 349, 664, 769, 1259, 1942, 2014
BsmBI CGTCTC 1 cut(s) 267
BsmFI GGGAC 3 cut(s) 633, 939, 963
BsmI GAATGC 1 cut(s) 473
BsnI GGCC 4 cut(s) 390, 520, 728, 1274
Bso31I GGTCTC 3 cut(s) 349, 1259, 1942
Bsp1286I GDGCHC 4 cut(s) 140, 372, 814, 1372
Bsp13I TCCGGA 1 cut(s) 265
Bsp1407I TGTACA 1 cut(s) 718
BspACI CCGC 5 cut(s) 145, 154, 169, 228, 863
BspANI GGCC 4 cut(s) 390, 520, 728, 1274
BspCNI CTCAG 5 cut(s) 446, 494, 658, 1702, 1929
BspDI ATCGAT 1 cut(s) 2049
BspEI TCCGGA 1 cut(s) 265
BspFNI CGCG 1 cut(s) 154
BspLI GGNNCC 5 cut(s) 870, 1126, 1231, 1273, 1937
BspMAI CTGCAG 2 cut(s) 841, 1900
BspPI GGATC 6 cut(s) 276, 863, 876, 1307, 1465, 1739
BspQI GCTCTTC 1 cut(s) 226
BspT107I GGYRCC 1 cut(s) 1229
BspTNI GGTCTC 3 cut(s) 349, 1259, 1942
BsrDI GCAATG 1 cut(s) 1290
BsrGI TGTACA 1 cut(s) 718
BsrI ACTGG 6 cut(s) 121, 296, 831, 957, 975, 1703
BssECI CCNNGG 4 cut(s) 140, 1128, 1269, 1820
BssT1I CCWWGG 1 cut(s) 1820
Bst2UI CCWGG 3 cut(s) 142, 1122, 1455
Bst4CI ACNGT 1 cut(s) 2008
Bst6I CTCTTC 4 cut(s) 27, 226, 304, 975
BstAUI TGTACA 1 cut(s) 718
BstC8I GCNNGC 2 cut(s) 228, 1154
BstDEI CTNAG 7 cut(s) 433, 481, 645, 674, 1710, 1916, 2079
BstDSI CCRYGG 1 cut(s) 1128
BstF5I GGATG 8 cut(s) 521, 667, 712, 737, 1378, 1450, 1647, 1975
BstFNI CGCG 1 cut(s) 154
BstMAI GTCTC 8 cut(s) 219, 267, 349, 664, 769, 1259, 1942, 2014
BstNI CCWGG 3 cut(s) 142, 1122, 1455
BstNSI RCATGY 2 cut(s) 725, 1156
BstSCI CCNGG 5 cut(s) 140, 358, 1120, 1268, 1453
BstSFI CTRYAG 3 cut(s) 837, 1506, 1896
BstSLI GKGCMC 2 cut(s) 372, 1372
BstUI CGCG 1 cut(s) 154
BstV1I GCAGC 4 cut(s) 135, 292, 295, 298
BstX2I RGATCY 1 cut(s) 868
BstYI RGATCY 1 cut(s) 868
Bsu15I ATCGAT 1 cut(s) 2049
Bsu36I CCTNAGG 1 cut(s) 1710
BsuI GTATCC 1 cut(s) 904
BsuRI GGCC 4 cut(s) 390, 520, 728, 1274
BsuTUI ATCGAT 1 cut(s) 2049
BtgI CCRYGG 1 cut(s) 1128
BtsCI GGATG 8 cut(s) 521, 667, 712, 737, 1378, 1450, 1647, 1975
BtsIMutI CAGTG 3 cut(s) 114, 289, 536
Cac8I GCNNGC 2 cut(s) 228, 1154
CaiI CAGNNNCTG 2 cut(s) 190, 1505
Cfr13I GGNCC 6 cut(s) 187, 294, 518, 1272, 1838, 2071
ClaI ATCGAT 1 cut(s) 2049
Csp6I GTAC 6 cut(s) 382, 719, 1133, 1230, 1471, 1985
CspCI CAANNNNNGTGG 4 cut(s) 306, 341, 678, 713
CviAII CATG 4 cut(s) 392, 722, 1153, 1997
CviQI GTAC 6 cut(s) 382, 719, 1133, 1230, 1471, 1985
DdeI CTNAG 7 cut(s) 433, 481, 645, 674, 1710, 1916, 2079
EaeI YGGCCR 2 cut(s) 388, 726
Eam1104I CTCTTC 4 cut(s) 27, 226, 304, 975
EarI CTCTTC 4 cut(s) 27, 226, 304, 975
EciI GGCGGA 1 cut(s) 158
Eco130I CCWWGG 1 cut(s) 1820
Eco24I GRGCYC 1 cut(s) 140
Eco31I GGTCTC 3 cut(s) 349, 1259, 1942
Eco47I GGWCC 4 cut(s) 187, 294, 1838, 2071
Eco57I CTGAAG 3 cut(s) 385, 784, 1703
Eco81I CCTNAGG 1 cut(s) 1710
EcoO109I RGGNCCY 3 cut(s) 187, 1272, 1838
EcoRII CCWGG 3 cut(s) 140, 1120, 1453
EcoT14I CCWWGG 1 cut(s) 1820
EcoT22I ATGCAT 2 cut(s) 1158, 1612
EcoT38I GRGCYC 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 1820
Esp3I CGTCTC 1 cut(s) 267
FaeI CATG 4 cut(s) 395, 725, 1156, 2000
FaqI GGGAC 3 cut(s) 633, 939, 963
FatI CATG 4 cut(s) 391, 721, 1152, 1996
FauI CCCGC 2 cut(s) 235, 856
FbaI TGATCA 3 cut(s) 181, 1420, 1999
Fnu4HI GCNGC 6 cut(s) 124, 146, 155, 306, 309, 312
FokI GGATG 8 cut(s) 508, 654, 719, 724, 1365, 1437, 1654, 1962
FriOI GRGCYC 1 cut(s) 140
Fsp4HI GCNGC 6 cut(s) 124, 146, 155, 306, 309, 312
FspBI CTAG 3 cut(s) 636, 1149, 1970
GluI GCNGC 6 cut(s) 124, 146, 155, 306, 309, 312
GsuI CTGGAG 1 cut(s) 1143
HaeIII GGCC 4 cut(s) 390, 520, 728, 1274
HapII CCGG 4 cut(s) 266, 335, 360, 1269
Hin1II CATG 4 cut(s) 395, 725, 1156, 2000
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HinfI GANTC 3 cut(s) 275, 338, 1502
HpaII CCGG 4 cut(s) 266, 335, 360, 1269
HphI GGTGA 3 cut(s) 924, 1088, 1094
Hpy166II GTNNAC 4 cut(s) 258, 995, 1179, 1370
Hpy188I TCNGA 7 cut(s) 105, 208, 1069, 1683, 1863, 2037, 2067
Hpy8I GTNNAC 4 cut(s) 258, 995, 1179, 1370
Hpy99I CGWCG 2 cut(s) 561, 2071
HpyAV CCTTC 7 cut(s) 15, 90, 555, 930, 1201, 1282, 1678
HpyCH4III ACNGT 1 cut(s) 2008
HpyCH4IV ACGT 2 cut(s) 260, 1473
HpyF3I CTNAG 7 cut(s) 433, 481, 645, 674, 1710, 1916, 2079
HpySE526I ACGT 2 cut(s) 260, 1473
Hsp92II CATG 4 cut(s) 395, 725, 1156, 2000
Kpn2I TCCGGA 1 cut(s) 265
KpnI GGTACC 1 cut(s) 1233
Ksp22I TGATCA 3 cut(s) 181, 1420, 1999
LguI GCTCTTC 1 cut(s) 226
LmnI GCTCC 8 cut(s) 171, 319, 817, 1124, 1345, 1490, 1711, 1776
Lsp1109I GCAGC 4 cut(s) 135, 292, 295, 298
LweI GCATC 7 cut(s) 1048, 1165, 1387, 1597, 1619, 1719, 1984
MaeI CTAG 3 cut(s) 636, 1149, 1970
MaeII ACGT 2 cut(s) 260, 1473
MaeIII GTNAC 2 cut(s) 791, 1826
MfeI CAATTG 1 cut(s) 1340
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 4 cut(s) 140, 372, 814, 1372
MlsI TGGCCA 1 cut(s) 728
MluNI TGGCCA 1 cut(s) 728
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 2 cut(s) 839, 2090
Mox20I TGGCCA 1 cut(s) 728
Mph1103I ATGCAT 2 cut(s) 1158, 1612
MroI TCCGGA 1 cut(s) 265
MscI TGGCCA 1 cut(s) 728
MseI TTAA 6 cut(s) 606, 744, 1356, 1464, 1623, 1656
MslI CAYNNNNRTG 2 cut(s) 177, 1161
Msp20I TGGCCA 1 cut(s) 728
MspA1I CMGCKG 1 cut(s) 126
MspI CCGG 4 cut(s) 266, 335, 360, 1269
MspR9I CCNGG 5 cut(s) 142, 360, 1122, 1270, 1455
MunI CAATTG 1 cut(s) 1340
Mva1269I GAATGC 1 cut(s) 473
MvaI CCWGG 3 cut(s) 142, 1122, 1455
MvnI CGCG 1 cut(s) 154
NciI CCSGG 2 cut(s) 360, 1270
NlaIII CATG 4 cut(s) 395, 725, 1156, 2000
NlaIV GGNNCC 5 cut(s) 870, 1126, 1231, 1273, 1937
NmeAIII GCCGAG 1 cut(s) 1969
NmuCI GTSAC 1 cut(s) 1826
NsiI ATGCAT 2 cut(s) 1158, 1612
NspI RCATGY 2 cut(s) 725, 1156
PaeI GCATGC 1 cut(s) 1156
PciI ACATGT 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 226
PctI GAATGC 1 cut(s) 473
PfeI GAWTC 2 cut(s) 338, 1502
PflMI CCANNNNNTGG 2 cut(s) 821, 2090
PfoI TCCNGGA 2 cut(s) 1120, 1453
PkrI GCNGC 6 cut(s) 125, 147, 156, 307, 310, 313
PleI GAGTC 1 cut(s) 283
PpsI GAGTC 1 cut(s) 283
PpuMI RGGWCCY 2 cut(s) 187, 1838
PscI ACATGT 1 cut(s) 721
PshBI ATTAAT 2 cut(s) 744, 1464
Psp5II RGGWCCY 2 cut(s) 187, 1838
Psp6I CCWGG 3 cut(s) 140, 1120, 1453
PspGI CCWGG 3 cut(s) 140, 1120, 1453
PspN4I GGNNCC 5 cut(s) 870, 1126, 1231, 1273, 1937
PspPI GGNCC 6 cut(s) 187, 294, 518, 1272, 1838, 2071
PspPPI RGGWCCY 2 cut(s) 187, 1838
PstI CTGCAG 2 cut(s) 841, 1900
PstNI CAGNNNCTG 2 cut(s) 190, 1505
PsuI RGATCY 1 cut(s) 868
PvuII CAGCTG 1 cut(s) 126
RsaI GTAC 6 cut(s) 383, 720, 1134, 1231, 1472, 1986
RsaNI GTAC 6 cut(s) 382, 719, 1133, 1230, 1471, 1985
RseI CAYNNNNRTG 2 cut(s) 177, 1161
SapI GCTCTTC 1 cut(s) 226
SaqAI TTAA 6 cut(s) 606, 744, 1356, 1464, 1623, 1656
SatI GCNGC 6 cut(s) 124, 146, 155, 306, 309, 312
Sau96I GGNCC 6 cut(s) 187, 294, 518, 1272, 1838, 2071
SchI GAGTC 1 cut(s) 284
ScrFI CCNGG 5 cut(s) 142, 360, 1122, 1270, 1455
SduI GDGCHC 4 cut(s) 140, 372, 814, 1372
SfaNI GCATC 7 cut(s) 1048, 1165, 1387, 1597, 1619, 1719, 1984
SfcI CTRYAG 3 cut(s) 837, 1506, 1896
SinI GGWCC 4 cut(s) 187, 294, 1838, 2071
SmiMI CAYNNNNRTG 2 cut(s) 177, 1161
SmlI CTYRAG 3 cut(s) 661, 894, 1842
SmoI CTYRAG 3 cut(s) 661, 894, 1842
SphI GCATGC 1 cut(s) 1156
SsiI CCGC 5 cut(s) 145, 154, 169, 228, 863
SspI AATATT 3 cut(s) 242, 943, 1387
SspMI CTAG 3 cut(s) 636, 1149, 1970
StyD4I CCNGG 5 cut(s) 140, 358, 1120, 1268, 1453
StyI CCWWGG 1 cut(s) 1820
TaaI ACNGT 1 cut(s) 2008
TaiI ACGT 2 cut(s) 263, 1476
TaqI TCGA 4 cut(s) 559, 850, 988, 2049
TatI WGTACW 1 cut(s) 718
TauI GCSGC 2 cut(s) 148, 157
TfiI GAWTC 2 cut(s) 338, 1502
Tru1I TTAA 6 cut(s) 606, 744, 1356, 1464, 1623, 1656
Tru9I TTAA 6 cut(s) 606, 744, 1356, 1464, 1623, 1656
TscAI CASTG 3 cut(s) 121, 296, 543
TseFI GTSAC 1 cut(s) 1826
TseI GCWGC 4 cut(s) 123, 305, 308, 311
Tsp45I GTSAC 1 cut(s) 1826
TspDTI ATGAA 6 cut(s) 726, 801, 1205, 1301, 1439, 2054
TspGWI ACGGA 4 cut(s) 1355, 1674, 1919, 2084
TspRI CASTG 3 cut(s) 121, 296, 543
Van91I CCANNNNNTGG 2 cut(s) 821, 2090
VneI GTGCAC 1 cut(s) 1368
VpaK11BI GGWCC 4 cut(s) 187, 294, 1838, 2071
VspI ATTAAT 2 cut(s) 744, 1464
XapI RAATTY 3 cut(s) 1012, 1594, 2026
XceI RCATGY 2 cut(s) 725, 1156
XcmI CCANNNNNNNNNTGG 2 cut(s) 1484, 1535
XspI CTAG 3 cut(s) 636, 1149, 1970
Zsp2I ATGCAT 2 cut(s) 1158, 1612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.