MD08G1060600.v1.1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001271
Physical Location & Seq
Reverse (-)
4808808 .. 4813086
4279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1060600.v1.1.491

Sequence Viewer

Length: 1902 bp
ATGCCTTCTTCTTCGTTTCCTCTTCACAAACCCTGCAAAACTACAACCTCCTCCTCCCCTGGTTGTTCTTCTTCTGCTACTTCCTCCGTTCCTGCTCTGAAACAGGACAGTGGTGGGGGCAGCTCCGACCAGAGCCCGCGGCGGCTCACCCGGCAGAGGAAGCTCCGCCACCTCAACAGCCAGGACCTGGGTTCTACCGTCCCTGAAAAATCTTACTCCTCGCCTACTTCTCCGGAATATTCGACCCAGAAGTCAAGATCGCCGGGTCGCGGGTCCAAGTCAATCCACTGGTCTTCTGCGCCTGCTGCTCCGCAGCCCTTGCCCCTCCCTGAATCGCACGTGACCCGGAAACCCGAATTTGCTAAAGATGGGTACTTTGGGAAGGTCAAAGGTCACACAACCTCAGGATCTGCAGCGCCGAGTTCTTATTCGAGTCAAGTTGCCACAACCTCAGGAGGATCAGCAGCACCAGCTAGTGCCTATTCGAACAATCGTCGAGGGGCACTCTCCCAAGATGCTAACAGTGGTGGTTTCTTTAATAACTTTGGTCTGAATATTCTTTCTAGGAAAGCTCTGGCGAATGGTGTATCAAGCCATCCGTTTAGCCCCCAAAGATCGAAAACGGTCGATAGTTATCCGATACTTGTTGCCCCCACTTCACCCGTTAATTGTTCAAGCAACTGCCATAGGGGAACGCCGCACGAATATGAAACTTGCAACACCAGCAGGAGTGCTCCAACAAGTCCTGCAGTTAGTCCCAGGAGATCAACTCCCACGGGGGACTTTTTTACTTCTTTTGGGGCTCCCCACGAACAAAATCAGGACGCCTTTCCTCCAAAATTATCCCCAAATAGACCTGCTCATAGCCCCAGCCATTCTCCTACATCTCGAAGTCCACGCTTCAACCAGAAAATTTCAAATGGAAATGCATTTCCTTTGCATTCTTTAGAGAGGGCTGAAAGTATTAGCCAAGTCAGTGCACACCCCTTGCCTCTGCCACCACAGCAATCTGTCATGCAGTACAATGCAGAAGCTCTATCCACATCATCAATGAAAGGTCAATGGCAGAAGGGAAAACTTATTGGGCGCGGCACCTTTGGTAGTGTTTATCTCGCAACCAACCGTGAAACCGGGGCCTTATGTGCAATGAAGGAAGTTGATCTCATCCCTGATGATCCTAAAGCTGCTGAATGTATAAGGCAATTGGAGCAGGAAATCAAAGTCCTCCGCACCCTAAAGCATCCAAATATTGTGCAGTATTATGGTAGTGAGATGACCGATGATCACTTCTACATATATTTGGAATATGTTCATCCTGGATCGATTAATAAATATGTCCAAGACCATATTGGAGTTATGACAGAATCTGTAGTTCGCAATTTTACTCGCCATATCCTCTCTGGGTTGGCTTTTTTGCACAACACGAAGACAGTTCACAGGGATATAAAGGGTGCAAATTTGCTTGTTGATGCGTCAGGCGTTGTTAAACTTGCCGATTTTGGGATGGCAAAACTTCTCAATGGGCAATCGTACAATCTTTCTTTGAAGGGCAGTCCATACTGGATGGCTCCTGAGGTCATAAGGGCTGCAATGCACAACAATGCTGATCCTGATCTTGCTTTGGCTGTTGATATATGGAGCTTGGGTTGTACCGTCATTGAGATGTTCAATGGAAAACCTCCATGGAGTGACTTTACAGGACCTCAAGCTATGTTCAAGGTTCTAAACACCATCCCGGATATACCAGAAACATTGTCTGCAGAGGGAAAGGACTTCCTCCAATGGTGCTTCCGTAGGAACCCTATGGAGAGACTATCAGCTAGTGACCTACTTGAGCATCCGTTTGTACGATATTCACCCGATAAATTGCATATATCGATCCCTCCACACGCTTCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

634

Amino Acids

68.78

Weight (kDa)

9.28

Isoelectric Point (pI)

56.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 355 - 616 3.8e-69 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 358 - 612 1.3e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 250
Acc36I ACCTGC 1 cut(s) 865
AccB1I GGYRCC 1 cut(s) 1091
AccB7I CCANNNNNTGG 1 cut(s) 187
AccII CGCG 3 cut(s) 139, 270, 1089
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 9 cut(s) 137, 139, 142, 166, 270, 311, 698, 1089, 1228
AclWI GGATC 6 cut(s) 415, 466, 1169, 1327, 1601, 1873
AcsI RAATTY 3 cut(s) 356, 912, 1456
AcvI CACGTG 1 cut(s) 340
AcyI GRCGYC 1 cut(s) 825
AfaI GTAC 5 cut(s) 374, 1022, 1532, 1651, 1848
AfiI CCNNNNNNNGG 4 cut(s) 156, 187, 269, 1500
AgsI TTSAA 6 cut(s) 675, 904, 918, 1546, 1669, 1717
AjnI CCWGG 5 cut(s) 58, 180, 186, 758, 1315
AluBI AGCT 9 cut(s) 123, 163, 473, 572, 1034, 1184, 1641, 1709, 1820
AluI AGCT 9 cut(s) 123, 163, 473, 572, 1034, 1184, 1641, 1709, 1820
Alw21I GWGCWC 2 cut(s) 736, 982
Alw26I GTCTC 1 cut(s) 1804
Alw44I GTGCAC 1 cut(s) 978
AlwI GGATC 6 cut(s) 415, 466, 1169, 1327, 1601, 1873
AlwNI CAGNNNCTG 3 cut(s) 187, 410, 1367
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 1 cut(s) 1134
ApaLI GTGCAC 1 cut(s) 978
ApeKI GCWGC 7 cut(s) 120, 305, 313, 413, 464, 1184, 1586
ApoI RAATTY 3 cut(s) 356, 912, 1456
AseI ATTAAT 1 cut(s) 1326
Asp700I GAANNNNTTC 1 cut(s) 1308
AspLEI GCGC 3 cut(s) 301, 418, 1089
AspS9I GGNCC 4 cut(s) 184, 273, 1134, 1700
AsuC2I CCSGG 5 cut(s) 151, 264, 346, 1132, 1736
AsuHPI GGTGA 3 cut(s) 139, 651, 1848
AsuII TTCGAA 1 cut(s) 485
AvaII GGWCC 3 cut(s) 184, 273, 1700
AxyI CCTNAGG 3 cut(s) 403, 451, 1572
BaeGI GKGCMC 2 cut(s) 505, 982
BanI GGYRCC 1 cut(s) 1091
BanII GRGCYC 2 cut(s) 137, 805
BbrPI CACGTG 1 cut(s) 340
BbsI GAAGAC 2 cut(s) 285, 1433
Bbv12I GWGCWC 2 cut(s) 736, 982
BbvI GCAGC 7 cut(s) 132, 292, 325, 425, 476, 1171, 1573
BccI CCATC 5 cut(s) 362, 603, 1498, 1558, 1739
BciT130I CCWGG 5 cut(s) 60, 182, 188, 760, 1317
BclI TGATCA 1 cut(s) 1282
BcnI CCSGG 5 cut(s) 151, 264, 346, 1132, 1736
BcoDI GTCTC 1 cut(s) 1804
BfaI CTAG 3 cut(s) 474, 564, 1821
BfmI CTRYAG 4 cut(s) 411, 747, 1368, 1758
BfoI RGCGCY 1 cut(s) 419
BfuAI ACCTGC 1 cut(s) 865
Bme18I GGWCC 3 cut(s) 184, 273, 1700
BmgT120I GGNCC 4 cut(s) 184, 273, 1134, 1700
BmiI GGNNCC 6 cut(s) 274, 804, 1093, 1135, 1569, 1799
BmsI GCATC 4 cut(s) 505, 1249, 1459, 1846
BpiI GAAGAC 2 cut(s) 285, 1433
BplI GAGNNNNNCTC 4 cut(s) 489, 521, 754, 786
Bpu14I TTCGAA 1 cut(s) 485
BpuEI CTTGAG 2 cut(s) 1689, 1853
BpuMI CCSGG 5 cut(s) 151, 264, 346, 1132, 1736
Bsa29I ATCGAT 2 cut(s) 1322, 1877
BsaAI YACGTR 1 cut(s) 340
BsaBI GATNNNNATC 2 cut(s) 633, 1611
BsaHI GRCGYC 1 cut(s) 825
BsaJI CCNNGG 7 cut(s) 58, 137, 187, 758, 774, 1131, 1682
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 1630, 1660
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 187, 269, 1500
Bse1I ACTGG 2 cut(s) 293, 1565
Bse21I CCTNAGG 3 cut(s) 403, 451, 1572
Bse3DI GCAATG 2 cut(s) 1152, 1596
Bse8I GATNNNNATC 2 cut(s) 633, 1611
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 5 cut(s) 60, 182, 188, 760, 1317
BseCI ATCGAT 2 cut(s) 1322, 1877
BseDI CCNNGG 7 cut(s) 58, 137, 187, 758, 774, 1131, 1682
BseGI GGATG 8 cut(s) 595, 1164, 1240, 1312, 1509, 1569, 1731, 1837
BseJI GATNNNNATC 2 cut(s) 633, 1611
BseLI CCNNNNNNNGG 4 cut(s) 156, 187, 269, 1500
BseMI GCAATG 2 cut(s) 1152, 1596
BseMII CTCAG 3 cut(s) 417, 465, 1563
BseNI ACTGG 2 cut(s) 293, 1565
BseRI GAGGAG 3 cut(s) 40, 43, 208
BseSI GKGCMC 2 cut(s) 505, 982
BseXI GCAGC 7 cut(s) 132, 292, 325, 425, 476, 1171, 1573
BseYI CCCAGC 1 cut(s) 869
BsgI GTGCAG 1 cut(s) 1274
Bsh1236I CGCG 3 cut(s) 139, 270, 1089
Bsh1285I CGRYCG 1 cut(s) 627
BshFI GGCC 1 cut(s) 1136
BshNI GGYRCC 1 cut(s) 1091
BshVI ATCGAT 2 cut(s) 1322, 1877
BsiEI CGRYCG 1 cut(s) 627
BsiHKAI GWGCWC 2 cut(s) 736, 982
BsiSI CCGG 6 cut(s) 151, 233, 263, 346, 1131, 1736
BslFI GGGAC 3 cut(s) 185, 741, 794
BslI CCNNNNNNNGG 4 cut(s) 156, 187, 269, 1500
BsmAI GTCTC 1 cut(s) 1804
BsmFI GGGAC 3 cut(s) 185, 741, 794
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 1 cut(s) 1136
Bsp119I TTCGAA 1 cut(s) 485
Bsp1286I GDGCHC 5 cut(s) 137, 505, 736, 805, 982
Bsp13I TCCGGA 1 cut(s) 232
Bsp19I CCATGG 1 cut(s) 1682
BspACI CCGC 9 cut(s) 137, 139, 142, 166, 270, 311, 698, 1089, 1228
BspANI GGCC 1 cut(s) 1136
BspCNI CTCAG 3 cut(s) 416, 464, 1564
BspDI ATCGAT 2 cut(s) 1322, 1877
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 3 cut(s) 139, 270, 1089
BspLI GGNNCC 6 cut(s) 274, 804, 1093, 1135, 1569, 1799
BspMAI CTGCAG 3 cut(s) 415, 751, 1762
BspMI ACCTGC 1 cut(s) 865
BspPI GGATC 6 cut(s) 415, 466, 1169, 1327, 1601, 1873
BspT104I TTCGAA 1 cut(s) 485
BspT107I GGYRCC 1 cut(s) 1091
BsrDI GCAATG 2 cut(s) 1152, 1596
BsrI ACTGG 2 cut(s) 293, 1565
BssECI CCNNGG 7 cut(s) 58, 137, 187, 758, 774, 1131, 1682
BssNI GRCGYC 1 cut(s) 825
BssT1I CCWWGG 1 cut(s) 1682
Bst2UI CCWGG 5 cut(s) 60, 182, 188, 760, 1317
Bst4CI ACNGT 7 cut(s) 110, 199, 524, 625, 1124, 1432, 1654
Bst6I CTCTTC 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 825
BstAPI GCANNNNNTGC 1 cut(s) 319
BstBAI YACGTR 1 cut(s) 340
BstBI TTCGAA 1 cut(s) 485
BstC8I GCNNGC 2 cut(s) 137, 303
BstDEI CTNAG 3 cut(s) 403, 451, 1572
BstDSI CCRYGG 3 cut(s) 137, 774, 1682
BstF5I GGATG 8 cut(s) 595, 1164, 1240, 1312, 1509, 1569, 1731, 1837
BstFNI CGCG 3 cut(s) 139, 270, 1089
BstH2I RGCGCY 1 cut(s) 419
BstHHI GCGC 3 cut(s) 301, 418, 1089
BstMAI GTCTC 1 cut(s) 1804
BstMCI CGRYCG 1 cut(s) 627
BstMWI GCNNNNNNNGC 9 cut(s) 151, 160, 305, 319, 470, 723, 1003, 1142, 1207
BstNI CCWGG 5 cut(s) 60, 182, 188, 760, 1317
BstSFI CTRYAG 4 cut(s) 411, 747, 1368, 1758
BstSLI GKGCMC 2 cut(s) 505, 982
BstUI CGCG 3 cut(s) 139, 270, 1089
BstV1I GCAGC 7 cut(s) 132, 292, 325, 425, 476, 1171, 1573
BstV2I GAAGAC 2 cut(s) 285, 1433
BstX2I RGATCY 1 cut(s) 407
BstYI RGATCY 1 cut(s) 407
Bsu15I ATCGAT 2 cut(s) 1322, 1877
Bsu36I CCTNAGG 3 cut(s) 403, 451, 1572
BsuRI GGCC 1 cut(s) 1136
BsuTUI ATCGAT 2 cut(s) 1322, 1877
BtgI CCRYGG 3 cut(s) 137, 774, 1682
BtsCI GGATG 8 cut(s) 595, 1164, 1240, 1312, 1509, 1569, 1731, 1837
BtsIMutI CAGTG 4 cut(s) 115, 286, 529, 982
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 2 cut(s) 137, 303
CaiI CAGNNNCTG 3 cut(s) 187, 410, 1367
CfoI GCGC 3 cut(s) 301, 418, 1089
Cfr13I GGNCC 4 cut(s) 184, 273, 1134, 1700
Cfr42I CCGCGG 1 cut(s) 140
ClaI ATCGAT 2 cut(s) 1322, 1877
CseI GACGC 2 cut(s) 833, 1461
Csp6I GTAC 5 cut(s) 373, 1021, 1531, 1650, 1847
CviAII CATG 2 cut(s) 1015, 1683
CviQI GTAC 5 cut(s) 373, 1021, 1531, 1650, 1847
DdeI CTNAG 3 cut(s) 403, 451, 1572
DrdI GACNNNNNNGTC 1 cut(s) 250
DseDI GACNNNNNNGTC 1 cut(s) 250
Eam1104I CTCTTC 1 cut(s) 27
EarI CTCTTC 1 cut(s) 27
EciI GGCGGA 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 1682
Eco24I GRGCYC 2 cut(s) 137, 805
Eco47I GGWCC 3 cut(s) 184, 273, 1700
Eco72I CACGTG 1 cut(s) 340
Eco81I CCTNAGG 3 cut(s) 403, 451, 1572
EcoO109I RGGNCCY 3 cut(s) 184, 1134, 1700
EcoRII CCWGG 5 cut(s) 58, 180, 186, 758, 1315
EcoT14I CCWWGG 1 cut(s) 1682
EcoT22I ATGCAT 1 cut(s) 931
EcoT38I GRGCYC 2 cut(s) 137, 805
ErhI CCWWGG 1 cut(s) 1682
FaeI CATG 2 cut(s) 1018, 1686
FaqI GGGAC 3 cut(s) 185, 741, 794
FatI CATG 2 cut(s) 1014, 1682
FauI CCCGC 2 cut(s) 144, 263
FbaI TGATCA 1 cut(s) 1282
FokI GGATG 8 cut(s) 582, 1151, 1227, 1299, 1516, 1576, 1718, 1824
FriOI GRGCYC 2 cut(s) 137, 805
FspBI CTAG 3 cut(s) 474, 564, 1821
GlaI GCGC 3 cut(s) 300, 417, 1088
GsaI CCCAGC 1 cut(s) 873
HaeII RGCGCY 1 cut(s) 419
HaeIII GGCC 1 cut(s) 1136
HapII CCGG 6 cut(s) 151, 233, 263, 346, 1131, 1736
HgaI GACGC 2 cut(s) 833, 1461
HhaI GCGC 3 cut(s) 301, 418, 1089
Hin1I GRCGYC 1 cut(s) 825
Hin1II CATG 2 cut(s) 1018, 1686
Hin6I GCGC 3 cut(s) 299, 416, 1087
HinP1I GCGC 3 cut(s) 299, 416, 1087
HinfI GANTC 3 cut(s) 332, 433, 1364
HpaII CCGG 6 cut(s) 151, 233, 263, 346, 1131, 1736
HphI GGTGA 3 cut(s) 139, 651, 1848
Hpy166II GTNNAC 3 cut(s) 896, 980, 1435
Hpy188I TCNGA 4 cut(s) 99, 127, 552, 639
Hpy188III TCNNGA 8 cut(s) 233, 255, 405, 453, 821, 888, 1571, 1610
Hpy8I GTNNAC 3 cut(s) 896, 980, 1435
Hpy99I CGWCG 1 cut(s) 498
HpyAV CCTTC 5 cut(s) 15, 376, 1063, 1144, 1540
HpyCH4III ACNGT 7 cut(s) 110, 199, 524, 625, 1124, 1432, 1654
HpyCH4IV ACGT 1 cut(s) 339
HpyF10VI GCNNNNNNNGC 9 cut(s) 151, 160, 305, 319, 470, 723, 1003, 1142, 1207
HpyF3I CTNAG 3 cut(s) 403, 451, 1572
HpySE526I ACGT 1 cut(s) 339
Hsp92I GRCGYC 1 cut(s) 825
Hsp92II CATG 2 cut(s) 1018, 1686
HspAI GCGC 3 cut(s) 299, 416, 1087
Kpn2I TCCGGA 1 cut(s) 232
Ksp22I TGATCA 1 cut(s) 1282
KspI CCGCGG 1 cut(s) 140
LmnI GCTCC 8 cut(s) 128, 168, 313, 739, 808, 1207, 1573, 1638
Lsp1109I GCAGC 7 cut(s) 132, 292, 325, 425, 476, 1171, 1573
LweI GCATC 4 cut(s) 505, 1249, 1459, 1846
MaeI CTAG 3 cut(s) 474, 564, 1821
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 4 cut(s) 340, 392, 1688, 1823
MboII GAAGA 6 cut(s) 3, 14, 60, 63, 285, 1438
MfeI CAATTG 1 cut(s) 1202
MflI RGATCY 1 cut(s) 407
MhlI GDGCHC 5 cut(s) 137, 505, 736, 805, 982
MluCI AATT 9 cut(s) 356, 667, 839, 912, 1202, 1378, 1456, 1865, 1897
MlyI GAGTC 1 cut(s) 442
MmeI TCCRAC 2 cut(s) 150, 761
Mph1103I ATGCAT 1 cut(s) 931
MroI TCCGGA 1 cut(s) 232
MroXI GAANNNNTTC 1 cut(s) 1308
MseI TTAA 4 cut(s) 537, 666, 1326, 1485
MslI CAYNNNNRTG 3 cut(s) 705, 1599, 1661
MspA1I CMGCKG 1 cut(s) 139
MspI CCGG 6 cut(s) 151, 233, 263, 346, 1131, 1736
MunI CAATTG 1 cut(s) 1202
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 5 cut(s) 60, 182, 188, 760, 1317
MvnI CGCG 3 cut(s) 139, 270, 1089
MwoI GCNNNNNNNGC 9 cut(s) 151, 160, 305, 319, 470, 723, 1003, 1142, 1207
NciI CCSGG 5 cut(s) 151, 264, 346, 1132, 1736
NcoI CCATGG 1 cut(s) 1682
NlaIII CATG 2 cut(s) 1018, 1686
NlaIV GGNNCC 6 cut(s) 274, 804, 1093, 1135, 1569, 1799
NmeAIII GCCGAG 1 cut(s) 444
NmuCI GTSAC 4 cut(s) 340, 392, 1688, 1823
NsiI ATGCAT 1 cut(s) 931
NspV TTCGAA 1 cut(s) 485
PctI GAATGC 1 cut(s) 940
PdmI GAANNNNTTC 1 cut(s) 1308
PfeI GAWTC 2 cut(s) 332, 1364
PflMI CCANNNNNTGG 1 cut(s) 187
PfoI TCCNGGA 2 cut(s) 1315, 1734
PleI GAGTC 1 cut(s) 441
PmaCI CACGTG 1 cut(s) 340
PmlI CACGTG 1 cut(s) 340
PpsI GAGTC 1 cut(s) 441
Ppu21I YACGTR 1 cut(s) 340
PpuMI RGGWCCY 2 cut(s) 184, 1700
PshBI ATTAAT 1 cut(s) 1326
Psp5II RGGWCCY 2 cut(s) 184, 1700
Psp6I CCWGG 5 cut(s) 58, 180, 186, 758, 1315
PspCI CACGTG 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 869
PspGI CCWGG 5 cut(s) 58, 180, 186, 758, 1315
PspN4I GGNNCC 6 cut(s) 274, 804, 1093, 1135, 1569, 1799
PspPI GGNCC 4 cut(s) 184, 273, 1134, 1700
PspPPI RGGWCCY 2 cut(s) 184, 1700
PstI CTGCAG 3 cut(s) 415, 751, 1762
PstNI CAGNNNCTG 3 cut(s) 187, 410, 1367
PsuI RGATCY 1 cut(s) 407
RsaI GTAC 5 cut(s) 374, 1022, 1532, 1651, 1848
RsaNI GTAC 5 cut(s) 373, 1021, 1531, 1650, 1847
RseI CAYNNNNRTG 3 cut(s) 705, 1599, 1661
SacII CCGCGG 1 cut(s) 140
SaqAI TTAA 4 cut(s) 537, 666, 1326, 1485
Sau96I GGNCC 4 cut(s) 184, 273, 1134, 1700
SchI GAGTC 1 cut(s) 442
SduI GDGCHC 5 cut(s) 137, 505, 736, 805, 982
SfaNI GCATC 4 cut(s) 505, 1249, 1459, 1846
SfcI CTRYAG 4 cut(s) 411, 747, 1368, 1758
Sfr303I CCGCGG 1 cut(s) 140
SfuI TTCGAA 1 cut(s) 485
SgrBI CCGCGG 1 cut(s) 140
SinI GGWCC 3 cut(s) 184, 273, 1700
SmiMI CAYNNNNRTG 3 cut(s) 705, 1599, 1661
SmlI CTYRAG 2 cut(s) 1704, 1832
SmoI CTYRAG 2 cut(s) 1704, 1832
Sse9I AATT 9 cut(s) 356, 667, 839, 912, 1202, 1378, 1456, 1865, 1897
SsiI CCGC 9 cut(s) 137, 139, 142, 166, 270, 311, 698, 1089, 1228
SspI AATATT 3 cut(s) 239, 556, 1249
SspMI CTAG 3 cut(s) 474, 564, 1821
StyI CCWWGG 1 cut(s) 1682
TaaI ACNGT 7 cut(s) 110, 199, 524, 625, 1124, 1432, 1654
TaiI ACGT 1 cut(s) 342
TaqI TCGA 9 cut(s) 242, 431, 485, 496, 617, 627, 889, 1322, 1877
TaqII GACCGA 1 cut(s) 1292
TasI AATT 9 cut(s) 356, 667, 839, 912, 1202, 1378, 1456, 1865, 1897
TatI WGTACW 1 cut(s) 1020
TauI GCSGC 4 cut(s) 142, 145, 700, 1092
TfiI GAWTC 2 cut(s) 332, 1364
Tru1I TTAA 4 cut(s) 537, 666, 1326, 1485
Tru9I TTAA 4 cut(s) 537, 666, 1326, 1485
TscAI CASTG 4 cut(s) 115, 293, 529, 982
TseFI GTSAC 4 cut(s) 340, 392, 1688, 1823
TseI GCWGC 7 cut(s) 120, 305, 313, 413, 464, 1184, 1586
Tsp45I GTSAC 4 cut(s) 340, 392, 1688, 1823
TspDTI ATGAA 4 cut(s) 723, 1067, 1163, 1301
TspGWI ACGGA 4 cut(s) 76, 588, 1781, 1830
TspRI CASTG 4 cut(s) 115, 293, 529, 982
Van91I CCANNNNNTGG 1 cut(s) 187
VneI GTGCAC 1 cut(s) 978
VpaK11BI GGWCC 3 cut(s) 184, 273, 1700
VspI ATTAAT 1 cut(s) 1326
XapI RAATTY 3 cut(s) 356, 912, 1456
XcmI CCANNNNNNNNNTGG 2 cut(s) 1346, 1397
XmnI GAANNNNTTC 1 cut(s) 1308
XspI CTAG 3 cut(s) 474, 564, 1821
Zsp2I ATGCAT 1 cut(s) 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.