Prupe.7G137700_v2.0.a1

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0001271
Physical Location & Seq
Reverse (-)
15426518 .. 15430559
4042 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G137700.1

Sequence Viewer

Length: 1938 bp
ATGGGTTGGTTGTCTAACATTTCCTTCTCTTCTTCCTCCTCGTCGTCCCCTTCTTCGTTTTCTTCGGTGTTCAGATCATCGTCTGCCGAGTCGCCAAAACAGAAGGCGAGCGATGACGGAGGATGGATGTTCCGATCGAGGAGGAAGCTCACGCGACAGAGGAAGCAGCTTTTGATCAGCGGCGGCGGCGGCGGAGACCAAGACGGTGGAGGTGGAGCCGTGAACAAATGTCCGAGTTCGCCGTTGCCAGAAATTGCATCGTCGCTGGCGAATCCGCAGCCGCTTCCTTTGCCAGAATTGGCGAAAGAGAGGTGCCGGAAAGAGAGGGCCTCGGGTTGCAATTCCGGTGATTTTGGAGACAGTCGATTGCCGTCTCCCAAGGCCGGTGACGGTGCCGCCTTGAACACTCCGATTCAAAATGTGATTGCTTCCCGAGACACAAGAAAGAATAATGACCATGCATTCGAGACAAGATCAAGGTCTAGGTGGAAGAATCGAGATATGAAGGGTTTAGAGATCTATAAAAAAGACTTCAGGCTTGATGTTCCTATTTGGAGCGCTCCTGCTAGTCCTTTCTCAAGTCCTACATTCAGCCCGCACAGACCCAGTGCTGGTGATCTGCTCCCTCACATTAATCCTATGGGAAATCAAGTCTGGTCTGCCCTTGAGATGCCCACTTCCGATATGAATCCCGGGCTCCCTCCCCCTGCCTTCTCAGATTACTCCATAGTTAGCAATGATAATTCCCCTATACACAGTCCTTCAAATAGAAGTCCCTGCCGAAACCCCAGAAGCCCACTAGGCTCAACATCACCATTGCATACCAAATTATCAATTGAAGCCTCCACAGCCCGGCGCGAGGGTAATGGTTATGTTGAATTCCATCCATTGCCTTTGCCTCCTGGAGCAAGCCTTAGCCTACCTTCGCCACAAGCTCTACTCACACCCAAACCAGAGTCCCAGCCGGTCAAAGGACAATGGCAAAAGGGAAAGCTTATTGGGCGTGGCACGTTTGGAAGTGTTTATGTTGCTACCAATAGAGAAACTGGAGCCTTATGTGCAATGAAGGAAGTTGAATTATTTCCTGATGACCCAAAATCTGCAGAATGTATAAAGCAGCTACAGCAGGAAATCAAAGTTCTCAGCCAGCTGAAGCATCCTAATATTGTGCAGTACTATGGAAGTGAAATAGTTGATGATCGATTTTATATATATCTGGAGTACGTCCATCCTGGTTCTATTAACAAATATGTCCATGAACATTGTGGAGCCATAACGGAAGCTGTTGTTCGCAGTTTCACTCGCCATATACTCTCTGGGTTGGCTTACTTGCACAGCACAAAGACAATACACAGGGACATCAAAGGGGCTAATTTGCTTGTCGATTCGTGTGGGGTTGTCAAGCTAGCTGACTTTGGAATGGCAAAACATCTTTCGGGACACGTGGGAAATCTTTCTCTGAAGGGAAGTCCATACTGGATGGCTCCAGAGCTCATGCAAGCTGTGATGCATAAAGATAACAACTCTGATCTAGCGCTTGCTGTTGATATCTGGAGTTTGGGTTGCACTATTATTGAGATGTTCACTGGAAAACCTCCTTGGAGTGAGTATGAAGGGGCTGCAGCCATGTTTAAAGTTATGAAAGATCTTCCACCAATTCCTGAAACATTGTCATCTGAGGGTAAGGATTTCCTGAGATGCTGCTTTCGAAGAAATCCTGCAGAGAGGCCAACTGCTGCCGTCTTATTAGAACATCGATTCTTAAAAAATTCTCAGCCGCAGGATGTCCAGTCTGTCACCCCGTCTGTCACCCAGGCATCTAGTGCGATGGATTACATGGATAAACTCCGTCCAGTCGAACTTGAAAGAAAATACGATCAGTCACTGATGATTCCGGGCATACTTGGACCCCAGACCAGTGCATTCCCAATTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

646

Amino Acids

70.63

Weight (kDa)

8.99

Isoelectric Point (pI)

62.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 312, 392
AccII CGCG 2 cut(s) 154, 858
AcsI RAATTY 2 cut(s) 878, 1768
AcuI CTGAAG 3 cut(s) 517, 1172, 1481
AcvI CACGTG 1 cut(s) 1444
AfaI GTAC 2 cut(s) 1175, 1223
AfeI AGCGCT 2 cut(s) 559, 1536
AfiI CCNNNNNNNGG 5 cut(s) 383, 611, 852, 859, 971
AflIII ACRYGT 1 cut(s) 1441
AgsI TTSAA 7 cut(s) 403, 416, 765, 839, 878, 1076, 1865
AjnI CCWGG 3 cut(s) 901, 1231, 1812
AjuI GAANNNNNNNTTGG 2 cut(s) 1582, 1614
Alw21I GWGCWC 1 cut(s) 1494
Alw26I GTCTC 5 cut(s) 189, 351, 378, 429, 461
AlwNI CAGNNNCTG 1 cut(s) 1885
Ama87I CYCGRG 3 cut(s) 331, 432, 692
Aor51HI AGCGCT 2 cut(s) 559, 1536
AoxI GGCC 3 cut(s) 327, 381, 1727
ApeKI GCWGC 7 cut(s) 166, 277, 1117, 1619, 1622, 1701, 1736
ApoI RAATTY 2 cut(s) 878, 1768
ArsI GACNNNNNNTTYG 2 cut(s) 446, 478
AseI ATTAAT 1 cut(s) 633
Asp700I GAANNNNTTC 2 cut(s) 1080, 1453
AspLEI GCGC 3 cut(s) 560, 858, 1537
AspS9I GGNCC 2 cut(s) 327, 1907
AsuC2I CCSGG 4 cut(s) 693, 694, 853, 1896
AsuHPI GGTGA 6 cut(s) 359, 398, 626, 804, 1789, 1801
AsuII TTCGAA 1 cut(s) 1708
AsuNHI GCTAGC 1 cut(s) 1405
AvaI CYCGRG 3 cut(s) 331, 432, 692
AvaII GGWCC 1 cut(s) 1907
BanI GGYRCC 2 cut(s) 312, 392
BanII GRGCYC 2 cut(s) 699, 1494
BbrPI CACGTG 1 cut(s) 1444
Bbv12I GWGCWC 1 cut(s) 1494
BbvI GCAGC 7 cut(s) 178, 289, 1129, 1606, 1634, 1688, 1723
BccI CCATC 5 cut(s) 117, 891, 1236, 1474, 1822
BceAI ACGGC 4 cut(s) 203, 226, 355, 1724
BciT130I CCWGG 3 cut(s) 903, 1233, 1814
BclI TGATCA 1 cut(s) 174
BcnI CCSGG 4 cut(s) 693, 694, 853, 1896
BcoDI GTCTC 5 cut(s) 189, 351, 378, 429, 461
BfaI CTAG 6 cut(s) 483, 567, 800, 1406, 1532, 1821
BfmI CTRYAG 4 cut(s) 1101, 1121, 1620, 1719
BfoI RGCGCY 2 cut(s) 561, 1538
BglI GCCNNNNNGGC 1 cut(s) 801
BglII AGATCT 2 cut(s) 516, 1645
BmcAI AGTACT 1 cut(s) 1175
Bme1390I CCNGG 7 cut(s) 693, 694, 853, 903, 1233, 1814, 1896
Bme18I GGWCC 1 cut(s) 1907
BmeT110I CYCGRG 3 cut(s) 331, 432, 692
BmgT120I GGNCC 2 cut(s) 327, 1907
BmiI GGNNCC 8 cut(s) 217, 314, 394, 698, 1051, 1270, 1485, 1909
BmrFI CCNGG 7 cut(s) 693, 694, 853, 903, 1233, 1814, 1896
BmrI ACTGGG 1 cut(s) 600
BmsI GCATC 6 cut(s) 266, 660, 1165, 1497, 1688, 1826
BmtI GCTAGC 1 cut(s) 1409
BmuI ACTGGG 1 cut(s) 600
BplI GAGNNNNNCTC 2 cut(s) 314, 346
BpmI CTGGAG 5 cut(s) 924, 1068, 1238, 1470, 1573
Bpu10I CCTNAGC 1 cut(s) 914
Bpu14I TTCGAA 1 cut(s) 1708
BpuEI CTTGAG 2 cut(s) 562, 686
BpuMI CCSGG 4 cut(s) 693, 694, 853, 1896
Bsa29I ATCGAT 2 cut(s) 1201, 1756
BsaAI YACGTR 1 cut(s) 1444
BsaBI GATNNNNATC 1 cut(s) 687
BsaI GGTCTC 1 cut(s) 189
BsaJI CCNNGG 5 cut(s) 330, 378, 692, 1598, 1812
BsaWI WCCGGW 1 cut(s) 344
BsaXI ACNNNNNCTCC 2 cut(s) 1546, 1576
Bsc4I CCNNNNNNNGG 5 cut(s) 383, 611, 852, 859, 971
Bse118I RCCGGY 2 cut(s) 383, 964
Bse1I ACTGG 7 cut(s) 606, 1051, 1481, 1591, 1789, 1853, 1917
Bse3DI GCAATG 4 cut(s) 742, 815, 887, 1068
Bse8I GATNNNNATC 1 cut(s) 687
BseBI CCWGG 3 cut(s) 903, 1233, 1814
BseCI ATCGAT 2 cut(s) 1201, 1756
BseDI CCNNGG 5 cut(s) 330, 378, 692, 1598, 1812
BseGI GGATG 7 cut(s) 128, 132, 883, 1156, 1228, 1485, 1789
BseJI GATNNNNATC 1 cut(s) 687
BseLI CCNNNNNNNGG 5 cut(s) 383, 611, 852, 859, 971
BseMI GCAATG 4 cut(s) 742, 815, 887, 1068
BseMII CTCAG 5 cut(s) 729, 1156, 1668, 1685, 1787
BseNI ACTGG 7 cut(s) 606, 1051, 1481, 1591, 1789, 1853, 1917
BseRI GAGGAG 2 cut(s) 28, 154
BseXI GCAGC 7 cut(s) 178, 289, 1129, 1606, 1634, 1688, 1723
BseYI CCCAGC 1 cut(s) 960
BsgI GTGCAG 1 cut(s) 1190
Bsh1236I CGCG 2 cut(s) 154, 858
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 3 cut(s) 329, 383, 1729
BshNI GGYRCC 2 cut(s) 312, 392
BshVI ATCGAT 2 cut(s) 1201, 1756
BsiEI CGRYCG 1 cut(s) 137
BsiHKAI GWGCWC 1 cut(s) 1494
BsiHKCI CYCGRG 3 cut(s) 331, 432, 692
BsiSI CCGG 7 cut(s) 316, 345, 384, 693, 853, 965, 1895
BslFI GGGAC 5 cut(s) 31, 759, 943, 1370, 1452
BslI CCNNNNNNNGG 5 cut(s) 383, 611, 852, 859, 971
BsmAI GTCTC 5 cut(s) 189, 351, 378, 429, 461
BsmBI CGTCTC 1 cut(s) 378
BsmFI GGGAC 5 cut(s) 31, 759, 943, 1370, 1452
BsmI GAATGC 2 cut(s) 461, 1922
BsnI GGCC 3 cut(s) 329, 383, 1729
Bso31I GGTCTC 1 cut(s) 189
BsoBI CYCGRG 3 cut(s) 331, 432, 692
Bsp119I TTCGAA 1 cut(s) 1708
Bsp1286I GDGCHC 2 cut(s) 699, 1494
BspANI GGCC 3 cut(s) 329, 383, 1729
BspCNI CTCAG 5 cut(s) 728, 1155, 1669, 1686, 1786
BspDI ATCGAT 2 cut(s) 1201, 1756
BspFNI CGCG 2 cut(s) 154, 858
BspLI GGNNCC 8 cut(s) 217, 314, 394, 698, 1051, 1270, 1485, 1909
BspMAI CTGCAG 3 cut(s) 1105, 1624, 1723
BspOI GCTAGC 1 cut(s) 1409
BspT104I TTCGAA 1 cut(s) 1708
BspT107I GGYRCC 2 cut(s) 312, 392
BspTNI GGTCTC 1 cut(s) 189
BsrDI GCAATG 4 cut(s) 742, 815, 887, 1068
BsrFI RCCGGY 2 cut(s) 383, 964
BsrI ACTGG 7 cut(s) 606, 1051, 1481, 1591, 1789, 1853, 1917
BssAI RCCGGY 2 cut(s) 383, 964
BssECI CCNNGG 5 cut(s) 330, 378, 692, 1598, 1812
BssT1I CCWWGG 2 cut(s) 378, 1598
Bst2UI CCWGG 3 cut(s) 903, 1233, 1814
Bst4CI ACNGT 4 cut(s) 206, 362, 392, 758
Bst6I CTCTTC 1 cut(s) 34
BstAPI GCANNNNNTGC 1 cut(s) 1823
BstBAI YACGTR 1 cut(s) 1444
BstBI TTCGAA 1 cut(s) 1708
BstC8I GCNNGC 8 cut(s) 109, 267, 596, 910, 1148, 1407, 1500, 1539
BstDEI CTNAG 6 cut(s) 715, 914, 1142, 1677, 1694, 1773
BstF5I GGATG 7 cut(s) 128, 132, 883, 1156, 1228, 1485, 1789
BstFNI CGCG 2 cut(s) 154, 858
BstH2I RGCGCY 2 cut(s) 561, 1538
BstHHI GCGC 3 cut(s) 560, 858, 1537
BstMAI GTCTC 5 cut(s) 189, 351, 378, 429, 461
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 9 cut(s) 186, 189, 289, 801, 848, 1000, 1058, 1123, 1823
BstNI CCWGG 3 cut(s) 903, 1233, 1814
BstSCI CCNGG 7 cut(s) 691, 692, 851, 901, 1231, 1812, 1894
BstSFI CTRYAG 4 cut(s) 1101, 1121, 1620, 1719
BstUI CGCG 2 cut(s) 154, 858
BstV1I GCAGC 7 cut(s) 178, 289, 1129, 1606, 1634, 1688, 1723
BstX2I RGATCY 2 cut(s) 516, 1645
BstXI CCANNNNNNTGG 1 cut(s) 206
BstYI RGATCY 2 cut(s) 516, 1645
Bsu15I ATCGAT 2 cut(s) 1201, 1756
BsuRI GGCC 3 cut(s) 329, 383, 1729
BsuTUI ATCGAT 2 cut(s) 1201, 1756
BtgZI GCGATG 2 cut(s) 126, 1841
BtsCI GGATG 7 cut(s) 128, 132, 883, 1156, 1228, 1485, 1789
BtsIMutI CAGTG 4 cut(s) 613, 1584, 1883, 1924
Cac8I GCNNGC 8 cut(s) 109, 267, 596, 910, 1148, 1407, 1500, 1539
CaiI CAGNNNCTG 1 cut(s) 1885
CfoI GCGC 3 cut(s) 560, 858, 1537
Cfr10I RCCGGY 2 cut(s) 383, 964
Cfr13I GGNCC 2 cut(s) 327, 1907
Cfr9I CCCGGG 1 cut(s) 692
ClaI ATCGAT 2 cut(s) 1201, 1756
Csp6I GTAC 2 cut(s) 1174, 1222
CviAII CATG 5 cut(s) 458, 1256, 1495, 1627, 1837
CviQI GTAC 2 cut(s) 1174, 1222
DdeI CTNAG 6 cut(s) 715, 914, 1142, 1677, 1694, 1773
DraI TTTAAA 1 cut(s) 1633
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
EciI GGCGGA 1 cut(s) 207
Ecl136II GAGCTC 1 cut(s) 1492
Eco130I CCWWGG 2 cut(s) 378, 1598
Eco24I GRGCYC 2 cut(s) 699, 1494
Eco31I GGTCTC 1 cut(s) 189
Eco32I GATATC 1 cut(s) 1549
Eco47I GGWCC 1 cut(s) 1907
Eco47III AGCGCT 2 cut(s) 559, 1536
Eco53kI GAGCTC 1 cut(s) 1492
Eco57I CTGAAG 3 cut(s) 517, 1172, 1481
Eco72I CACGTG 1 cut(s) 1444
Eco88I CYCGRG 3 cut(s) 331, 432, 692
EcoICRI GAGCTC 1 cut(s) 1492
EcoO109I RGGNCCY 1 cut(s) 327
EcoRI GAATTC 1 cut(s) 878
EcoRII CCWGG 3 cut(s) 901, 1231, 1812
EcoRV GATATC 1 cut(s) 1549
EcoT14I CCWWGG 2 cut(s) 378, 1598
EcoT22I ATGCAT 2 cut(s) 463, 1512
EcoT38I GRGCYC 2 cut(s) 699, 1494
ErhI CCWWGG 2 cut(s) 378, 1598
Esp3I CGTCTC 1 cut(s) 378
FaeI CATG 5 cut(s) 461, 1259, 1498, 1630, 1840
FaqI GGGAC 5 cut(s) 31, 759, 943, 1370, 1452
FatI CATG 5 cut(s) 457, 1255, 1494, 1626, 1836
FauI CCCGC 1 cut(s) 603
FbaI TGATCA 1 cut(s) 174
FokI GGATG 7 cut(s) 135, 139, 870, 1143, 1215, 1492, 1796
FriOI GRGCYC 2 cut(s) 699, 1494
FspBI CTAG 6 cut(s) 483, 567, 800, 1406, 1532, 1821
GlaI GCGC 3 cut(s) 559, 857, 1536
GsaI CCCAGC 1 cut(s) 964
GsuI CTGGAG 5 cut(s) 924, 1068, 1238, 1470, 1573
HaeII RGCGCY 2 cut(s) 561, 1538
HaeIII GGCC 3 cut(s) 329, 383, 1729
HapII CCGG 7 cut(s) 316, 345, 384, 693, 853, 965, 1895
HhaI GCGC 3 cut(s) 560, 858, 1537
Hin1II CATG 5 cut(s) 461, 1259, 1498, 1630, 1840
Hin6I GCGC 3 cut(s) 558, 856, 1535
HinP1I GCGC 3 cut(s) 558, 856, 1535
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 9 cut(s) 89, 271, 412, 493, 688, 956, 1385, 1758, 1891
HpaII CCGG 7 cut(s) 316, 345, 384, 693, 853, 965, 1895
HphI GGTGA 6 cut(s) 359, 398, 626, 804, 1789, 1801
Hpy166II GTNNAC 2 cut(s) 223, 1584
Hpy188I TCNGA 9 cut(s) 74, 134, 234, 411, 682, 718, 1461, 1528, 1678
Hpy8I GTNNAC 2 cut(s) 223, 1584
Hpy99I CGWCG 2 cut(s) 46, 265
HpyCH4III ACNGT 4 cut(s) 206, 362, 392, 758
HpyCH4IV ACGT 3 cut(s) 1010, 1224, 1443
HpyF10VI GCNNNNNNNGC 9 cut(s) 186, 189, 289, 801, 848, 1000, 1058, 1123, 1823
HpyF3I CTNAG 6 cut(s) 715, 914, 1142, 1677, 1694, 1773
HpySE526I ACGT 3 cut(s) 1010, 1224, 1443
Hsp92II CATG 5 cut(s) 461, 1259, 1498, 1630, 1840
HspAI GCGC 3 cut(s) 558, 856, 1535
Ksp22I TGATCA 1 cut(s) 174
LmnI GCTCC 9 cut(s) 215, 555, 565, 627, 702, 905, 1049, 1268, 1489
Lsp1109I GCAGC 7 cut(s) 178, 289, 1129, 1606, 1634, 1688, 1723
LweI GCATC 6 cut(s) 266, 660, 1165, 1497, 1688, 1826
MaeI CTAG 6 cut(s) 483, 567, 800, 1406, 1532, 1821
MaeII ACGT 3 cut(s) 1010, 1224, 1443
MaeIII GTNAC 4 cut(s) 386, 1795, 1807, 1881
MboII GAAGA 7 cut(s) 21, 24, 45, 54, 502, 1640, 1722
MfeI CAATTG 1 cut(s) 834
MflI RGATCY 2 cut(s) 516, 1645
MhlI GDGCHC 2 cut(s) 699, 1494
MlyI GAGTC 2 cut(s) 98, 965
Mph1103I ATGCAT 2 cut(s) 463, 1512
MroXI GAANNNNTTC 2 cut(s) 1080, 1453
MseI TTAA 4 cut(s) 633, 1242, 1632, 1763
MspA1I CMGCKG 2 cut(s) 180, 1150
MspI CCGG 7 cut(s) 316, 345, 384, 693, 853, 965, 1895
MspR9I CCNGG 7 cut(s) 693, 694, 853, 903, 1233, 1814, 1896
MunI CAATTG 1 cut(s) 834
Mva1269I GAATGC 2 cut(s) 461, 1922
MvaI CCWGG 3 cut(s) 903, 1233, 1814
MvnI CGCG 2 cut(s) 154, 858
MwoI GCNNNNNNNGC 9 cut(s) 186, 189, 289, 801, 848, 1000, 1058, 1123, 1823
NciI CCSGG 4 cut(s) 693, 694, 853, 1896
NheI GCTAGC 1 cut(s) 1405
NlaIII CATG 5 cut(s) 461, 1259, 1498, 1630, 1840
NlaIV GGNNCC 8 cut(s) 217, 314, 394, 698, 1051, 1270, 1485, 1909
NmeAIII GCCGAG 1 cut(s) 112
NmuCI GTSAC 4 cut(s) 386, 1795, 1807, 1881
NsiI ATGCAT 2 cut(s) 463, 1512
NspV TTCGAA 1 cut(s) 1708
PcsI WCGNNNNNNNCGW 1 cut(s) 266
PctI GAATGC 2 cut(s) 461, 1922
PdmI GAANNNNTTC 2 cut(s) 1080, 1453
PfeI GAWTC 7 cut(s) 271, 412, 493, 688, 1385, 1758, 1891
PfoI TCCNGGA 1 cut(s) 901
Ple19I CGATCG 1 cut(s) 137
PleI GAGTC 2 cut(s) 97, 964
PmaCI CACGTG 1 cut(s) 1444
PmlI CACGTG 1 cut(s) 1444
PpsI GAGTC 2 cut(s) 97, 964
Ppu21I YACGTR 1 cut(s) 1444
PshBI ATTAAT 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 1494
Psp6I CCWGG 3 cut(s) 901, 1231, 1812
PspCI CACGTG 1 cut(s) 1444
PspFI CCCAGC 1 cut(s) 960
PspGI CCWGG 3 cut(s) 901, 1231, 1812
PspN4I GGNNCC 8 cut(s) 217, 314, 394, 698, 1051, 1270, 1485, 1909
PspPI GGNCC 2 cut(s) 327, 1907
PstI CTGCAG 3 cut(s) 1105, 1624, 1723
PstNI CAGNNNCTG 1 cut(s) 1885
PsuI RGATCY 2 cut(s) 516, 1645
PvuI CGATCG 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 1150
RsaI GTAC 2 cut(s) 1175, 1223
RsaNI GTAC 2 cut(s) 1174, 1222
SacI GAGCTC 1 cut(s) 1494
SaqAI TTAA 4 cut(s) 633, 1242, 1632, 1763
Sau96I GGNCC 2 cut(s) 327, 1907
ScaI AGTACT 1 cut(s) 1175
SchI GAGTC 2 cut(s) 98, 965
ScrFI CCNGG 7 cut(s) 693, 694, 853, 903, 1233, 1814, 1896
SduI GDGCHC 2 cut(s) 699, 1494
SfaNI GCATC 6 cut(s) 266, 660, 1165, 1497, 1688, 1826
SfcI CTRYAG 4 cut(s) 1101, 1121, 1620, 1719
SfuI TTCGAA 1 cut(s) 1708
SinI GGWCC 1 cut(s) 1907
SmaI CCCGGG 1 cut(s) 694
SmlI CTYRAG 2 cut(s) 577, 665
SmoI CTYRAG 2 cut(s) 577, 665
SspI AATATT 1 cut(s) 1165
SspMI CTAG 6 cut(s) 483, 567, 800, 1406, 1532, 1821
SstI GAGCTC 1 cut(s) 1494
StyD4I CCNGG 7 cut(s) 691, 692, 851, 901, 1231, 1812, 1894
StyI CCWWGG 2 cut(s) 378, 1598
TaaI ACNGT 4 cut(s) 206, 362, 392, 758
TaiI ACGT 3 cut(s) 1013, 1227, 1446
TaqI TCGA 9 cut(s) 137, 364, 465, 496, 1201, 1383, 1708, 1756, 1857
TatI WGTACW 1 cut(s) 1173
TauI GCSGC 7 cut(s) 183, 186, 189, 192, 283, 398, 1780
TfiI GAWTC 7 cut(s) 271, 412, 493, 688, 1385, 1758, 1891
Tru1I TTAA 4 cut(s) 633, 1242, 1632, 1763
Tru9I TTAA 4 cut(s) 633, 1242, 1632, 1763
TscAI CASTG 4 cut(s) 613, 1591, 1890, 1924
TseFI GTSAC 4 cut(s) 386, 1795, 1807, 1881
TseI GCWGC 7 cut(s) 166, 277, 1117, 1619, 1622, 1701, 1736
Tsp45I GTSAC 4 cut(s) 386, 1795, 1807, 1881
TspDTI ATGAA 6 cut(s) 518, 701, 1079, 1272, 1626, 1655
TspGWI ACGGA 3 cut(s) 132, 1292, 1838
TspMI CCCGGG 1 cut(s) 692
TspRI CASTG 4 cut(s) 613, 1591, 1890, 1924
VpaK11BI GGWCC 1 cut(s) 1907
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 2 cut(s) 878, 1768
XcmI CCANNNNNNNNNTGG 2 cut(s) 1262, 1313
XmaI CCCGGG 1 cut(s) 692
XmnI GAANNNNTTC 2 cut(s) 1080, 1453
XspI CTAG 6 cut(s) 483, 567, 800, 1406, 1532, 1821
ZrmI AGTACT 1 cut(s) 1175
Zsp2I ATGCAT 2 cut(s) 463, 1512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.