pycom02g13460

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0001271
Physical Location & Seq
Forward (+)
9981499 .. 9990930
9432 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g13460.4

Sequence Viewer

Length: 2310 bp
ATGCCTTGTGAAATCCCATCCCAATTAATTATTTTCTCCAAATTCTCCTTCTCTCTTTGGCTGCCACGTGGCAGCTTCCCCACCCCCATCTCTTTTCCTTCTTTCTTTCTCTCCTTTCTCCCCGGAGTACTCTCTCTCAGCTCACTCATTCTCTTTCTCTCACACACTCTCTCCCTCTCCCCGAACCCACACACCCAAGATCGACAAAATGACGGCGAAGATGACACCACCACGTCCACCACGTTGAGTCTTTCTCCTTCCGTCTTTGTGAAGCCCAGGAAACCCAGCAAACTTCCTTGGCGAGCCTCGTGGCTTTTGAGCCAAACTTCGGCCAACTCTGACCACCACACGGCGCGTTTGAGGGTTTCCACCATGCGTTGGCTGTCTAACATTACCTTCTCTTCTTCTTCTTCTTCCTCCTCCTCGTCGGCGAAAGGGGAGTCGCCGAAAAAGAAGGCCAGCGATGACGGGGGAGGCTGGATGTTTCGGTCGAGGAGGAAGCTTACCCAACAGAGGAAGCAGCTTTTGATCGGTATTGGCGGTGGAAACCAGGAAGGTGGAGGAGGTGGAGTCATCAATAATCATAAATGCTCGGGTTCGAGTTCGTCGTTTCCGGAAACTACATCGGGGGCAGGGACTCCTCAGCCGCTGCCATTGCCGGAATCGGCCGCGGATTCGCGATGCAAAGAGAGGTGCCGGAGAGAGAGGTGCTCCGGATGCAGTTCCGGCGATTGTCGATTGCCCTCTCCCAAGGCCCGTGACGCTACCTCCTCCAACACTTCTATTCAGAGTGTGTTCGCTCGCCGGGAAACGCGAAAGAATAACGAGCATTGTTTTGAACCTAGGTCAAGGTCTACAAGGAGGAATCGAGGTAGGAATGGTTTAGGGAGCTATCCTAGTGACTTCCGGCTTGATGTTCCGAAACCTACTTGGAGTGCTCCGGCTAGTCCTTTCTCAAGTCCTACATTCAGCCCGCACAGACCCAGTGCTGGTGATCTGCTCCCACACCTTGTTCCTGTTGGAAATCAAGGCTGGTCTGCCCCCGAGATGCCCACTTACGATGTGCCTTCTGCGCACCCACCCTCTTCCTTCTCCGAATACTCCATCCAGAGCAATGATAATTCCCCTCTACAAAGTCCATCCAATAGAAGTCCCTGCCGAAACGTCAGAAGCCCACCAGGTTCAACATCGCCATTGCACCCAAGATTATCATTTGAAGCCCCCACTGTGCGGCGTGAGGTTAATGGTTATGCTGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGTCTTAGCCTACCATCACCACAAGCTATACTCACACCTAAACCAGAGTCCCAGCCAGTCAAGGGTCAATGGCAAAAGGGAAAGCTTATTGGACGTGGCACGTTCGGAAGTGTTTATGTTGCTACCAATACGGTTTTCGTTTGTAACAGAGAAACTGGAGCGTTATGTGCAATGAAGGAAGTCGAGTTATTTCCTGAGGACCCAAAATCCGCAGAGTGTATAAAGCAGCTACAGCAGGTTATCATCCGTTTACTCTCAATTTGTTCTAGGAATGTTGATGATCGATTTTTCATCTATCTGGAGTATGTCCATCCTGGTTCTATCAACAAATATGTCCATGAACATTGTGGAGCCATAACAGAAGCTGTTGTTCGTAATTTTACTCGCCATATTCTCTCTGGGTTGGCTTACTTGCACAGCACAAAGACAATACACAGGTACAATCACATTCTTAATCTAATAATAAAGAATTGCTCACCTACGCAAATTTTTATCATTGTCAAATGTGGAAGAATTGTTCTTGGTCTTTCGGGACACGTAGGTAATCTTTCTCTAAGGGGAAGCCCGTACTGGATGGCTCCAGAGCTGATGCAAGCTGTGATGCATAAAGATAGCAGCTCTGATCTGGCTCTTGCTGTTGATATATGGAGCTTGGGTTGCACTATTATTGAAATGTTCACCGGAAAACCTCCTTGGAGCGAGTACGAAGGGGCTGCAGCCATGTTTAAAGTTATGAAGGATGCGCCACCAATTCCTGAAACATTGTCACCTGAGGGTAAGGATTTCTTAAGATGCTGCTTCCAAAGAAATCCTGCAGAAAGGCCAACTGCTGCCATGTTATTAGAACATCGATTCTTAAAGAACTCACAGCAGCAAGATGCCCCGCCTGCCGCCCAGGCATCTAATGGGGTGAATTACGCGGATAAACCTCTGAGTCCAATGGAACTTGAAAGAAAATTTGATCAGTCGCTGGTGATTCCGGCCATACTTGGATCGCAGAGCAGTGCATTCCCGATTTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

770

Amino Acids

84.38

Weight (kDa)

9.36

Isoelectric Point (pI)

62.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1074
Acc36I ACCTGC 1 cut(s) 1513
AccB1I GGYRCC 1 cut(s) 693
AccB7I CCANNNNNTGG 1 cut(s) 378
AccI GTMKAC 1 cut(s) 854
AccII CGCG 5 cut(s) 355, 671, 679, 814, 2207
AccIII TCCGGA 2 cut(s) 613, 713
AclWI GGATC 1 cut(s) 2287
AcoI YGGCCR 3 cut(s) 330, 666, 2268
AcsI RAATTY 3 cut(s) 41, 1773, 2243
AcuI CTGAAG 1 cut(s) 1275
AcvI CACGTG 1 cut(s) 68
AfaI GTAC 4 cut(s) 129, 1727, 1856, 1991
AfiI CCNNNNNNNGG 7 cut(s) 328, 349, 378, 513, 989, 1230, 1349
AflII CTTAAG 1 cut(s) 2074
AflIII ACRYGT 1 cut(s) 1822
AgsI TTSAA 5 cut(s) 839, 1185, 1217, 1958, 2237
AjiI CACGTC 2 cut(s) 234, 1382
AjnI CCWGG 6 cut(s) 275, 549, 1177, 1279, 1600, 2181
AjuI GAANNNNNNNTTGG 6 cut(s) 32, 64, 1406, 1438, 1963, 1995
AloI GAACNNNNNNTCC 2 cut(s) 1788, 1820
Alw21I GWGCWC 2 cut(s) 713, 940
AlwI GGATC 1 cut(s) 2287
AlwNI CAGNNNCTG 3 cut(s) 649, 1652, 2257
Ama87I CYCGRG 2 cut(s) 592, 1043
Aor13HI TCCGGA 2 cut(s) 613, 713
AoxI GGCC 6 cut(s) 330, 456, 666, 753, 2108, 2268
ApoI RAATTY 3 cut(s) 41, 1773, 2243
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AseI ATTAAT 1 cut(s) 26
AspA2I CCTAGG 1 cut(s) 842
AspLEI GCGC 3 cut(s) 355, 1075, 2032
AspS9I GGNCC 2 cut(s) 754, 1486
AsuC2I CCSGG 2 cut(s) 123, 806
AsuHPI GGTGA 7 cut(s) 1004, 1296, 1755, 1957, 2046, 2209, 2272
AvaI CYCGRG 2 cut(s) 592, 1043
AvaII GGWCC 1 cut(s) 1486
AvrII CCTAGG 1 cut(s) 842
AxyI CCTNAGG 2 cut(s) 1482, 2058
BanI GGYRCC 1 cut(s) 693
BauI CACGAG 1 cut(s) 307
BbrPI CACGTG 1 cut(s) 68
Bbv12I GWGCWC 2 cut(s) 713, 940
BbvCI CCTCAGC 1 cut(s) 642
BccI CCATC 8 cut(s) 25, 95, 1112, 1147, 1269, 1309, 1605, 1855
BceAI ACGGC 2 cut(s) 229, 366
BcgI CGANNNNNNTGC 2 cut(s) 1982, 2016
BciT130I CCWGG 6 cut(s) 277, 551, 1179, 1281, 1602, 2183
BclI TGATCA 1 cut(s) 2248
BcnI CCSGG 2 cut(s) 123, 806
BfaI CTAG 4 cut(s) 843, 897, 945, 1554
BfmI CTRYAG 3 cut(s) 1517, 2001, 2100
BfrI CTTAAG 1 cut(s) 2074
BfuAI ACCTGC 1 cut(s) 1513
BglI GCCNNNNNGGC 1 cut(s) 2183
BlnI CCTAGG 1 cut(s) 842
BmcAI AGTACT 1 cut(s) 129
Bme1390I CCNGG 8 cut(s) 123, 277, 551, 806, 1179, 1281, 1602, 2183
Bme18I GGWCC 1 cut(s) 1486
BmeT110I CYCGRG 2 cut(s) 592, 1043
BmgBI CACGTC 2 cut(s) 234, 1382
BmgT120I GGNCC 2 cut(s) 754, 1486
BmiI GGNNCC 4 cut(s) 695, 1488, 1639, 1866
BmrFI CCNGG 8 cut(s) 123, 277, 551, 806, 1179, 1281, 1602, 2183
BmrI ACTGGG 1 cut(s) 978
BmsI GCATC 9 cut(s) 671, 707, 1038, 1866, 1878, 2017, 2069, 2155, 2195
BmuI ACTGGG 1 cut(s) 978
BplI GAGNNNNNCTC 4 cut(s) 238, 270, 695, 727
BpmI CTGGAG 4 cut(s) 1302, 1464, 1607, 1851
Bpu10I CCTNAGC 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 940
BpuMI CCSGG 2 cut(s) 123, 806
Bsa29I ATCGAT 2 cut(s) 1570, 2137
BsaAI YACGTR 2 cut(s) 68, 1825
BsaJI CCNNGG 8 cut(s) 121, 275, 296, 669, 750, 842, 1979, 2181
BsaWI WCCGGW 3 cut(s) 613, 713, 1967
BsaXI ACNNNNNCTCC 8 cut(s) 155, 185, 555, 585, 752, 782, 1927, 1957
Bsc4I CCNNNNNNNGG 7 cut(s) 328, 349, 378, 513, 989, 1230, 1349
Bse1I ACTGG 4 cut(s) 984, 1343, 1447, 1862
Bse21I CCTNAGG 2 cut(s) 1482, 2058
Bse3DI GCAATG 5 cut(s) 653, 1120, 1193, 1265, 1464
BseAI TCCGGA 2 cut(s) 613, 713
BseBI CCWGG 6 cut(s) 277, 551, 1179, 1281, 1602, 2183
BseCI ATCGAT 2 cut(s) 1570, 2137
BseDI CCNNGG 8 cut(s) 121, 275, 296, 669, 750, 842, 1979, 2181
BseLI CCNNNNNNNGG 7 cut(s) 328, 349, 378, 513, 989, 1230, 1349
BseMI GCAATG 5 cut(s) 653, 1120, 1193, 1265, 1464
BseMII CTCAG 5 cut(s) 151, 656, 1473, 2049, 2210
BseNI ACTGG 4 cut(s) 984, 1343, 1447, 1862
BseRI GAGGAG 6 cut(s) 409, 412, 508, 576, 630, 760
BseX3I CGGCCG 1 cut(s) 666
BseYI CCCAGC 2 cut(s) 284, 1338
Bsh1236I CGCG 5 cut(s) 355, 671, 679, 814, 2207
Bsh1285I CGRYCG 2 cut(s) 491, 669
BshFI GGCC 6 cut(s) 332, 458, 668, 755, 2110, 2270
BshNI GGYRCC 1 cut(s) 693
BshVI ATCGAT 2 cut(s) 1570, 2137
BsiEI CGRYCG 2 cut(s) 491, 669
BsiHKAI GWGCWC 2 cut(s) 713, 940
BsiHKCI CYCGRG 2 cut(s) 592, 1043
BslFI GGGAC 4 cut(s) 649, 1137, 1321, 1833
BslI CCNNNNNNNGG 7 cut(s) 328, 349, 378, 513, 989, 1230, 1349
BsmFI GGGAC 4 cut(s) 649, 1137, 1321, 1833
BsmI GAATGC 1 cut(s) 2294
BsnI GGCC 6 cut(s) 332, 458, 668, 755, 2110, 2270
BsoBI CYCGRG 2 cut(s) 592, 1043
Bsp1286I GDGCHC 2 cut(s) 713, 940
Bsp13I TCCGGA 2 cut(s) 613, 713
Bsp143I GATC 7 cut(s) 199, 528, 994, 1567, 1909, 2248, 2279
Bsp68I TCGCGA 1 cut(s) 679
BspANI GGCC 6 cut(s) 332, 458, 668, 755, 2110, 2270
BspCNI CTCAG 5 cut(s) 150, 655, 1474, 2050, 2211
BspDI ATCGAT 2 cut(s) 1570, 2137
BspEI TCCGGA 2 cut(s) 613, 713
BspFNI CGCG 5 cut(s) 355, 671, 679, 814, 2207
BspLI GGNNCC 4 cut(s) 695, 1488, 1639, 1866
BspMAI CTGCAG 2 cut(s) 2005, 2104
BspMI ACCTGC 1 cut(s) 1513
BspPI GGATC 1 cut(s) 2287
BspT107I GGYRCC 1 cut(s) 693
BspTI CTTAAG 1 cut(s) 2074
BsrDI GCAATG 5 cut(s) 653, 1120, 1193, 1265, 1464
BsrI ACTGG 4 cut(s) 984, 1343, 1447, 1862
BssECI CCNNGG 8 cut(s) 121, 275, 296, 669, 750, 842, 1979, 2181
BssMI GATC 7 cut(s) 199, 528, 994, 1567, 1909, 2248, 2279
BssSI CACGAG 1 cut(s) 307
BssT1I CCWWGG 4 cut(s) 296, 750, 842, 1979
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 6 cut(s) 277, 551, 1179, 1281, 1602, 2183
Bst4CI ACNGT 2 cut(s) 1228, 1420
Bst6I CTCTTC 2 cut(s) 406, 1090
BstAFI CTTAAG 1 cut(s) 2074
BstBAI YACGTR 2 cut(s) 68, 1825
BstC8I GCNNGC 6 cut(s) 303, 460, 802, 974, 1881, 2175
BstDEI CTNAG 7 cut(s) 137, 642, 1292, 1482, 1841, 2058, 2219
BstDSI CCRYGG 1 cut(s) 669
BstFNI CGCG 5 cut(s) 355, 671, 679, 814, 2207
BstHHI GCGC 3 cut(s) 355, 1075, 2032
BstKTI GATC 7 cut(s) 202, 531, 997, 1570, 1912, 2251, 2282
BstMBI GATC 7 cut(s) 199, 528, 994, 1567, 1909, 2248, 2279
BstMCI CGRYCG 2 cut(s) 491, 669
BstNI CCWGG 6 cut(s) 277, 551, 1179, 1281, 1602, 2183
BstSCI CCNGG 8 cut(s) 121, 275, 549, 804, 1177, 1279, 1600, 2181
BstSFI CTRYAG 3 cut(s) 1517, 2001, 2100
BstUI CGCG 5 cut(s) 355, 671, 679, 814, 2207
BstXI CCANNNNNNTGG 1 cut(s) 557
BstZI CGGCCG 1 cut(s) 666
Bsu15I ATCGAT 2 cut(s) 1570, 2137
Bsu36I CCTNAGG 2 cut(s) 1482, 2058
BsuRI GGCC 6 cut(s) 332, 458, 668, 755, 2110, 2270
BsuTUI ATCGAT 2 cut(s) 1570, 2137
BtgI CCRYGG 1 cut(s) 669
BtgZI GCGATG 3 cut(s) 477, 694, 1173
BtrI CACGTC 2 cut(s) 234, 1382
BtsI GCAGTG 1 cut(s) 2296
BtsIMutI CAGTG 3 cut(s) 991, 1224, 2296
BtuMI TCGCGA 1 cut(s) 679
BveI ACCTGC 1 cut(s) 1513
Cac8I GCNNGC 6 cut(s) 303, 460, 802, 974, 1881, 2175
CaiI CAGNNNCTG 3 cut(s) 649, 1652, 2257
CfoI GCGC 3 cut(s) 355, 1075, 2032
Cfr13I GGNCC 2 cut(s) 754, 1486
Cfr42I CCGCGG 1 cut(s) 672
ClaI ATCGAT 2 cut(s) 1570, 2137
CseI GACGC 1 cut(s) 770
CsiI ACCWGGT 1 cut(s) 1177
Csp6I GTAC 4 cut(s) 128, 1726, 1855, 1990
CviAII CATG 4 cut(s) 373, 1625, 2008, 2122
CviQI GTAC 4 cut(s) 128, 1726, 1855, 1990
DdeI CTNAG 7 cut(s) 137, 642, 1292, 1482, 1841, 2058, 2219
DpnI GATC 7 cut(s) 201, 530, 996, 1569, 1911, 2250, 2281
DpnII GATC 7 cut(s) 199, 528, 994, 1567, 1909, 2248, 2279
DraI TTTAAA 1 cut(s) 2014
EaeI YGGCCR 3 cut(s) 330, 666, 2268
EagI CGGCCG 1 cut(s) 666
Eam1104I CTCTTC 2 cut(s) 406, 1090
EarI CTCTTC 2 cut(s) 406, 1090
EclXI CGGCCG 1 cut(s) 666
Eco130I CCWWGG 4 cut(s) 296, 750, 842, 1979
Eco47I GGWCC 1 cut(s) 1486
Eco52I CGGCCG 1 cut(s) 666
Eco57I CTGAAG 1 cut(s) 1275
Eco72I CACGTG 1 cut(s) 68
Eco81I CCTNAGG 2 cut(s) 1482, 2058
Eco88I CYCGRG 2 cut(s) 592, 1043
EcoO109I RGGNCCY 1 cut(s) 1486
EcoRII CCWGG 6 cut(s) 275, 549, 1177, 1279, 1600, 2181
EcoT14I CCWWGG 4 cut(s) 296, 750, 842, 1979
EcoT22I ATGCAT 1 cut(s) 1893
ErhI CCWWGG 4 cut(s) 296, 750, 842, 1979
FaeI CATG 4 cut(s) 376, 1628, 2011, 2125
FalI AAGNNNNNCTT 2 cut(s) 2057, 2089
FaqI GGGAC 4 cut(s) 649, 1137, 1321, 1833
FatI CATG 4 cut(s) 372, 1624, 2007, 2121
FauI CCCGC 2 cut(s) 981, 2178
FbaI TGATCA 1 cut(s) 2248
FblI GTMKAC 1 cut(s) 854
FspBI CTAG 4 cut(s) 843, 897, 945, 1554
FspI TGCGCA 1 cut(s) 1074
GlaI GCGC 3 cut(s) 354, 1074, 2031
GsaI CCCAGC 2 cut(s) 288, 1342
GsuI CTGGAG 4 cut(s) 1302, 1464, 1607, 1851
HaeIII GGCC 6 cut(s) 332, 458, 668, 755, 2110, 2270
HgaI GACGC 1 cut(s) 770
HhaI GCGC 3 cut(s) 355, 1075, 2032
Hin1II CATG 4 cut(s) 376, 1628, 2011, 2125
Hin6I GCGC 3 cut(s) 353, 1073, 2030
HinP1I GCGC 3 cut(s) 353, 1073, 2030
HindIII AAGCTT 2 cut(s) 500, 1370
HphI GGTGA 7 cut(s) 1004, 1296, 1755, 1957, 2046, 2209, 2272
Hpy166II GTNNAC 4 cut(s) 237, 855, 1538, 1965
Hpy188I TCNGA 8 cut(s) 340, 789, 921, 1096, 1169, 1394, 1909, 2220
Hpy8I GTNNAC 4 cut(s) 237, 855, 1538, 1965
Hpy99I CGWCG 2 cut(s) 430, 610
HpyCH4III ACNGT 2 cut(s) 1228, 1420
HpyCH4IV ACGT 7 cut(s) 67, 233, 242, 1164, 1381, 1388, 1824
HpyF3I CTNAG 7 cut(s) 137, 642, 1292, 1482, 1841, 2058, 2219
HpySE526I ACGT 7 cut(s) 67, 233, 242, 1164, 1381, 1388, 1824
Hsp92II CATG 4 cut(s) 376, 1628, 2011, 2125
HspAI GCGC 3 cut(s) 353, 1073, 2030
Kpn2I TCCGGA 2 cut(s) 613, 713
Ksp22I TGATCA 1 cut(s) 2248
KspI CCGCGG 1 cut(s) 672
Kzo9I GATC 7 cut(s) 199, 528, 994, 1567, 1909, 2248, 2279
LweI GCATC 9 cut(s) 671, 707, 1038, 1866, 1878, 2017, 2069, 2155, 2195
MabI ACCWGGT 1 cut(s) 1177
MaeI CTAG 4 cut(s) 843, 897, 945, 1554
MaeII ACGT 7 cut(s) 67, 233, 242, 1164, 1381, 1388, 1824
MaeIII GTNAC 4 cut(s) 758, 899, 1430, 2052
MalI GATC 7 cut(s) 201, 530, 996, 1569, 1911, 2250, 2281
MboI GATC 7 cut(s) 199, 528, 994, 1567, 1909, 2248, 2279
MboII GAAGA 8 cut(s) 230, 393, 396, 399, 402, 405, 1077, 1809
MhlI GDGCHC 2 cut(s) 713, 940
MlyI GAGTC 6 cut(s) 256, 449, 579, 631, 1343, 2230
MmeI TCCRAC 2 cut(s) 798, 1000
Mph1103I ATGCAT 1 cut(s) 1893
MroI TCCGGA 2 cut(s) 613, 713
MseI TTAA 6 cut(s) 26, 1242, 1740, 2013, 2075, 2144
MspA1I CMGCKG 2 cut(s) 649, 671
MspCI CTTAAG 1 cut(s) 2074
MspR9I CCNGG 8 cut(s) 123, 277, 551, 806, 1179, 1281, 1602, 2183
Mva1269I GAATGC 1 cut(s) 2294
MvaI CCWGG 6 cut(s) 277, 551, 1179, 1281, 1602, 2183
MvnI CGCG 5 cut(s) 355, 671, 679, 814, 2207
NciI CCSGG 2 cut(s) 123, 806
NdeII GATC 7 cut(s) 199, 528, 994, 1567, 1909, 2248, 2279
NlaIII CATG 4 cut(s) 376, 1628, 2011, 2125
NlaIV GGNNCC 4 cut(s) 695, 1488, 1639, 1866
NmuCI GTSAC 3 cut(s) 758, 899, 2052
NruI TCGCGA 1 cut(s) 679
NsbI TGCGCA 1 cut(s) 1074
NsiI ATGCAT 1 cut(s) 1893
PcsI WCGNNNNNNNCGW 2 cut(s) 239, 605
PctI GAATGC 1 cut(s) 2294
PfeI GAWTC 5 cut(s) 662, 674, 865, 2139, 2263
PflMI CCANNNNNTGG 1 cut(s) 378
PfoI TCCNGGA 1 cut(s) 1279
PleI GAGTC 6 cut(s) 255, 448, 578, 631, 1342, 2229
PmaCI CACGTG 1 cut(s) 68
PmlI CACGTG 1 cut(s) 68
PpsI GAGTC 6 cut(s) 255, 448, 578, 631, 1342, 2229
Ppu21I YACGTR 2 cut(s) 68, 1825
PpuMI RGGWCCY 1 cut(s) 1486
PshBI ATTAAT 1 cut(s) 26
Psp5II RGGWCCY 1 cut(s) 1486
Psp6I CCWGG 6 cut(s) 275, 549, 1177, 1279, 1600, 2181
PspCI CACGTG 1 cut(s) 68
PspFI CCCAGC 2 cut(s) 284, 1338
PspGI CCWGG 6 cut(s) 275, 549, 1177, 1279, 1600, 2181
PspN4I GGNNCC 4 cut(s) 695, 1488, 1639, 1866
PspPI GGNCC 2 cut(s) 754, 1486
PspPPI RGGWCCY 1 cut(s) 1486
PstI CTGCAG 2 cut(s) 2005, 2104
PstNI CAGNNNCTG 3 cut(s) 649, 1652, 2257
RruI TCGCGA 1 cut(s) 679
RsaI GTAC 4 cut(s) 129, 1727, 1856, 1991
RsaNI GTAC 4 cut(s) 128, 1726, 1855, 1990
SacII CCGCGG 1 cut(s) 672
SaqAI TTAA 6 cut(s) 26, 1242, 1740, 2013, 2075, 2144
Sau3AI GATC 7 cut(s) 199, 528, 994, 1567, 1909, 2248, 2279
Sau96I GGNCC 2 cut(s) 754, 1486
ScaI AGTACT 1 cut(s) 129
SchI GAGTC 6 cut(s) 256, 449, 579, 631, 1343, 2230
ScrFI CCNGG 8 cut(s) 123, 277, 551, 806, 1179, 1281, 1602, 2183
SduI GDGCHC 2 cut(s) 713, 940
SexAI ACCWGGT 1 cut(s) 1177
SfaNI GCATC 9 cut(s) 671, 707, 1038, 1866, 1878, 2017, 2069, 2155, 2195
SfcI CTRYAG 3 cut(s) 1517, 2001, 2100
Sfr303I CCGCGG 1 cut(s) 672
SgrBI CCGCGG 1 cut(s) 672
SinI GGWCC 1 cut(s) 1486
SmlI CTYRAG 2 cut(s) 955, 2074
SmoI CTYRAG 2 cut(s) 955, 2074
SspMI CTAG 4 cut(s) 843, 897, 945, 1554
StyD4I CCNGG 8 cut(s) 121, 275, 549, 804, 1177, 1279, 1600, 2181
StyI CCWWGG 4 cut(s) 296, 750, 842, 1979
TaaI ACNGT 2 cut(s) 1228, 1420
TaiI ACGT 7 cut(s) 70, 236, 245, 1167, 1384, 1391, 1827
TaqI TCGA 8 cut(s) 202, 491, 599, 736, 868, 1470, 1570, 2137
TaqII GACCGA 1 cut(s) 477
TatI WGTACW 1 cut(s) 127
TauI GCSGC 4 cut(s) 649, 671, 1234, 2180
TfiI GAWTC 5 cut(s) 662, 674, 865, 2139, 2263
Tru1I TTAA 6 cut(s) 26, 1242, 1740, 2013, 2075, 2144
Tru9I TTAA 6 cut(s) 26, 1242, 1740, 2013, 2075, 2144
TscAI CASTG 3 cut(s) 991, 1231, 2296
TseFI GTSAC 3 cut(s) 758, 899, 2052
Tsp45I GTSAC 3 cut(s) 758, 899, 2052
TspDTI ATGAA 4 cut(s) 1475, 1567, 1641, 2036
TspGWI ACGGA 2 cut(s) 250, 1523
TspRI CASTG 3 cut(s) 991, 1231, 2296
Van91I CCANNNNNTGG 1 cut(s) 378
Vha464I CTTAAG 1 cut(s) 2074
VpaK11BI GGWCC 1 cut(s) 1486
VspI ATTAAT 1 cut(s) 26
XapI RAATTY 3 cut(s) 41, 1773, 2243
XcmI CCANNNNNNNNNTGG 3 cut(s) 1631, 1682, 2189
XmaJI CCTAGG 1 cut(s) 842
XmiI GTMKAC 1 cut(s) 854
XspI CTAG 4 cut(s) 843, 897, 945, 1554
ZrmI AGTACT 1 cut(s) 129
Zsp2I ATGCAT 1 cut(s) 1893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.