RchiOBHm_Chr6g0299851

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0001271
Physical Location & Seq
Reverse (-)
60849326 .. 60854460
5135 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26924

Sequence Viewer

Length: 2088 bp
ATGCCTTCTTCTTCTTCTGCTCCATCTCAATCTCATCAAAAACGAACCTTTTTTACATCGTCACCTTCTACTCCTGCTTCCTCCTCCTCCTCCTCTGTCTCAGCTGTGAGACCCGGGATCAACAGAAGCGGCAGTGATGTTAACAGCCCACGGCGGCTCACGCGCCAGAGGAGGCTGCGTTACCTCACGAGCCCCGAGGATCTCGACTCTTACGGGGTCCCGGAGAAGTCTCGCTCATCGCCCACCTCGCCTGAATATTCGACCCGTAGATCACGCTCTCCGTGTGGCGAGTCAAACCCAAATTCACGCTCTCCCGGTGGCGAGTCGGCCCCATGTTCTCGGTCTTCGGTCGGTGACTCGGCCCGAAAGTCATGCTCCTCTTCTTTCGTTGCTGGGCCTCAAGGCCCACAACCCTTGCCGCTTCCGGAGAACAACCGGAGACCCGACTGCGTCTCTAAAGATAACAACTTCTTCAGCCCTGTGGTCGGCAGGAAGAGCGTCGATCAAGCTGCCACAAGCTCATCATCACCGGCGAAATCTTCCAAGAACCGATGCCGAGATGTAAAAAATTCCTGCGGGGGTGGGGTTGGGAGTGGGGGTTTTAATTTCCATTTGGGTTTGAATGTTCAATCTAGGACTACTAGGAGTGCTCCACAGACTGTTTTTAGCTCAAGTCATCATCCTGTTAGCCCACAAAGATCAAACACAGTGGATTTTTTGCCTTGTCAGTCACATGTCGCTGGCAACTATTATAACAGGGGAATGGGAATGGCAGTGTCACCGGAGGCTAACTTTGAACGTGAAACTTACAACTTGAGCAGGAGTGCTCCAACCAGTGTTCTCTCAAGTCCTGCGGTTAGCCCGCGCCGATCAACCACTGGGGATCCTTTTCCTTTTTTCATGACTCATCAAGATCAATTTCAGGATGGTGCTACCTTTCCCTCGTTTTTGTCACCAAACAAGCCTACACTCAGTCCCACTCATTCTCCACTCCATAGCCCTACGCCTCGAAGTCCACGCTTCAGCCCCAATGCTTCAAACTATGAGAGTAGTCTTGCCAATGCTCACCCGTTGCCTCTGCCTCCTGGAGCCGCAGTGCCAGCACAGCCGCCCTCTATCACTATGCATCAAAATAATTTAGGAACCGTATCATCAATGAAAGGTCAATGGCAGAAGGGGAAACTTATCGGACGGGGTACCTTTGGTAGTGTTTATCTTGCAACCAACCGAGGAACTGGGGCCTTGTGTGCAATGAAGGAAGTTGATCTCATTCCTGATGATCCTAAATCTGCAGAAAGTATAAAGCAATTGGAGCAGGAAATTAAAGTCCTCCGTACACTAAAGCATCCAAATATTGTGCAGTACTATGGTAGTGAAGTGATTGATGATCATTTCTACATATATTTAGAATATGTTCACCCCGGATCAATAAATAAATATGTCCGAGACCATATTGGAGCTATGACTGAATCCGTAATCCGCAATTTTACCCGCCATATCCTCCGTGGGTTGGCTTTTTTGCATGACACGAAGACAATTCACAGGGATATTAAAGGTGCAAATTTGCTCGTTGATGCATCAGGTGTTGTCAAACTTGCCGATTTTGGGATGGCAAAACATCTTGGTGCGCATTCATACAATCTTTCTTTGAAGGGCAGCCCATACTGGATGGCTCCTGAGGTCATAAAGGCTGTGATACAGAATGATGGTGATCCTAATCTTGCTTTGGCTGTTGATATATGGAGCTTGGGTTGTACCATCATCGAGATGTTCAATGGAAAACCGCCTTGGAGTGAATTTGAAGGGCCACAAGCTATGTTCAAGGTTCTGCACAGAACCCCGGATATACCAGAAAAATTATCTGCAGAAGGAAAGGAATTCCTCAGTTTGTGCTTCAAAAGGAACCCCGCAGAGAGGCCATCAGCTAAGAAGCTACTTGATCATCCTTTTGTACGAAATTCAAATGATCAAAATGTCTCATCTTATACTCGGGCTTTTTCTATGGTAATGGATAAATTGAATAGCCCGACGGACCATATTAAGCAAAAACTTGGTCGAATGCTGGTCTATCCACGCACTTCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

695

Amino Acids

75.85

Weight (kDa)

9.64

Isoelectric Point (pI)

63.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 390 - 651 3.4e-67 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 393 - 646 1.2e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
Acc16I TGCGCA 1 cut(s) 1629
Acc65I GGTACC 1 cut(s) 1196
AccB1I GGYRCC 1 cut(s) 1196
AccII CGCG 2 cut(s) 163, 865
AccIII TCCGGA 1 cut(s) 424
AclWI GGATC 7 cut(s) 125, 207, 878, 891, 1274, 1432, 1706
AcsI RAATTY 6 cut(s) 301, 568, 1561, 1796, 1877, 1957
AcuI CTGAAG 2 cut(s) 457, 1006
AfaI GTAC 5 cut(s) 1198, 1336, 1364, 1756, 1953
AfiI CCNNNNNNNGG 4 cut(s) 485, 1510, 1605, 1914
AflIII ACRYGT 1 cut(s) 733
AjnI CCWGG 1 cut(s) 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 1771, 1803
AluBI AGCT 9 cut(s) 104, 509, 519, 669, 1460, 1746, 1814, 1925, 1933
AluI AGCT 9 cut(s) 104, 509, 519, 669, 1460, 1746, 1814, 1925, 1933
Alw21I GWGCWC 2 cut(s) 652, 829
Alw26I GTCTC 7 cut(s) 103, 103, 234, 433, 457, 1440, 1981
AlwI GGATC 7 cut(s) 125, 207, 878, 891, 1274, 1432, 1706
Ama87I CYCGRG 3 cut(s) 113, 194, 1989
Aor13HI TCCGGA 1 cut(s) 424
AoxI GGCC 7 cut(s) 327, 360, 395, 403, 1239, 1805, 1916
ApeKI GCWGC 3 cut(s) 175, 509, 1656
ApoI RAATTY 6 cut(s) 301, 568, 1561, 1796, 1877, 1957
ArsI GACNNNNNNTTYG 2 cut(s) 2038, 2070
Asp700I GAANNNNTTC 1 cut(s) 1413
Asp718I GGTACC 1 cut(s) 1196
AspLEI GCGC 3 cut(s) 165, 867, 1630
AspS9I GGNCC 8 cut(s) 217, 328, 361, 395, 404, 1239, 1805, 2032
AsuC2I CCSGG 6 cut(s) 114, 115, 221, 315, 1422, 1841
AsuHPI GGTGA 8 cut(s) 54, 365, 519, 771, 945, 1058, 1409, 1721
AvaI CYCGRG 3 cut(s) 113, 194, 1989
AvaII GGWCC 2 cut(s) 217, 2032
AxyI CCTNAGG 1 cut(s) 1677
BamHI GGATCC 1 cut(s) 883
BanI GGYRCC 1 cut(s) 1196
BanII GRGCYC 1 cut(s) 194
BauI CACGAG 1 cut(s) 187
BbsI GAAGAC 2 cut(s) 336, 1538
Bbv12I GWGCWC 2 cut(s) 652, 829
BbvI GCAGC 3 cut(s) 162, 496, 1668
BccI CCATC 7 cut(s) 31, 920, 1603, 1663, 1700, 1766, 1927
BceAI ACGGC 1 cut(s) 167
BcgI CGANNNNNNTGC 4 cut(s) 354, 388, 491, 525
BciT130I CCWGG 1 cut(s) 1086
BclI TGATCA 3 cut(s) 1387, 1939, 1966
BcnI CCSGG 6 cut(s) 114, 115, 221, 315, 1422, 1841
BcoDI GTCTC 7 cut(s) 103, 103, 234, 433, 457, 1440, 1981
BfaI CTAG 2 cut(s) 633, 642
BfmI CTRYAG 2 cut(s) 1290, 1863
BisI GCNGC 8 cut(s) 130, 155, 176, 419, 510, 1092, 1109, 1657
BlsI GCNGC 8 cut(s) 131, 156, 177, 420, 511, 1093, 1110, 1658
BmcAI AGTACT 1 cut(s) 1364
Bme1390I CCNGG 7 cut(s) 114, 115, 221, 315, 1086, 1422, 1841
Bme18I GGWCC 2 cut(s) 217, 2032
BmeT110I CYCGRG 3 cut(s) 113, 194, 1989
BmgT120I GGNCC 8 cut(s) 217, 328, 361, 395, 404, 1239, 1805, 2032
BmrFI CCNGG 7 cut(s) 114, 115, 221, 315, 1086, 1422, 1841
BmrI ACTGGG 2 cut(s) 888, 1245
BmsI GCATC 5 cut(s) 542, 1135, 1354, 1564, 1586
BmuI ACTGGG 2 cut(s) 888, 1245
BpiI GAAGAC 2 cut(s) 336, 1538
BpmI CTGGAG 1 cut(s) 1107
BpuEI CTTGAG 4 cut(s) 384, 655, 829, 835
BpuMI CCSGG 6 cut(s) 114, 115, 221, 315, 1422, 1841
BsaBI GATNNNNATC 2 cut(s) 1710, 1716
BsaI GGTCTC 3 cut(s) 103, 433, 1440
BsaJI CCNNGG 8 cut(s) 113, 149, 195, 1228, 1420, 1504, 1787, 1839
BsaWI WCCGGW 3 cut(s) 424, 435, 781
Bsc4I CCNNNNNNNGG 4 cut(s) 485, 1510, 1605, 1914
Bse118I RCCGGY 1 cut(s) 529
Bse1I ACTGG 4 cut(s) 834, 883, 1240, 1670
Bse21I CCTNAGG 1 cut(s) 1677
Bse3DI GCAATG 1 cut(s) 1257
Bse8I GATNNNNATC 2 cut(s) 1710, 1716
BseAI TCCGGA 1 cut(s) 424
BseBI CCWGG 1 cut(s) 1086
BseDI CCNNGG 8 cut(s) 113, 149, 195, 1228, 1420, 1504, 1787, 1839
BseGI GGATG 6 cut(s) 679, 931, 1345, 1614, 1674, 1942
BseJI GATNNNNATC 2 cut(s) 1710, 1716
BseLI CCNNNNNNNGG 4 cut(s) 485, 1510, 1605, 1914
BseMI GCAATG 1 cut(s) 1257
BseMII CTCAG 4 cut(s) 114, 985, 1668, 1897
BseNI ACTGG 4 cut(s) 834, 883, 1240, 1670
BseRI GAGGAG 6 cut(s) 73, 76, 79, 82, 184, 367
BseXI GCAGC 3 cut(s) 162, 496, 1668
BseYI CCCAGC 1 cut(s) 392
BsgI GTGCAG 2 cut(s) 1379, 1814
Bsh1236I CGCG 2 cut(s) 163, 865
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 7 cut(s) 329, 362, 397, 405, 1241, 1807, 1918
BshNI GGYRCC 1 cut(s) 1196
BsiEI CGRYCG 1 cut(s) 351
BsiHKAI GWGCWC 2 cut(s) 652, 829
BsiHKCI CYCGRG 3 cut(s) 113, 194, 1989
BsiSI CCGG 9 cut(s) 114, 221, 315, 425, 436, 530, 782, 1422, 1841
BslFI GGGAC 2 cut(s) 203, 960
BslI CCNNNNNNNGG 4 cut(s) 485, 1510, 1605, 1914
BsmAI GTCTC 7 cut(s) 103, 103, 234, 433, 457, 1440, 1981
BsmBI CGTCTC 1 cut(s) 457
BsmFI GGGAC 2 cut(s) 203, 960
BsmI GAATGC 2 cut(s) 1630, 2064
BsnI GGCC 7 cut(s) 329, 362, 397, 405, 1241, 1807, 1918
Bso31I GGTCTC 3 cut(s) 103, 433, 1440
BsoBI CYCGRG 3 cut(s) 113, 194, 1989
Bsp1286I GDGCHC 3 cut(s) 194, 652, 829
Bsp13I TCCGGA 1 cut(s) 424
BspANI GGCC 7 cut(s) 329, 362, 397, 405, 1241, 1807, 1918
BspCNI CTCAG 4 cut(s) 113, 984, 1669, 1896
BspEI TCCGGA 1 cut(s) 424
BspFNI CGCG 2 cut(s) 163, 865
BspHI TCATGA 1 cut(s) 900
BspMAI CTGCAG 2 cut(s) 1294, 1867
BspPI GGATC 7 cut(s) 125, 207, 878, 891, 1274, 1432, 1706
BspQI GCTCTTC 1 cut(s) 488
BspT107I GGYRCC 1 cut(s) 1196
BspTNI GGTCTC 3 cut(s) 103, 433, 1440
BsrDI GCAATG 1 cut(s) 1257
BsrFI RCCGGY 1 cut(s) 529
BsrI ACTGG 4 cut(s) 834, 883, 1240, 1670
BssAI RCCGGY 1 cut(s) 529
BssECI CCNNGG 8 cut(s) 113, 149, 195, 1228, 1420, 1504, 1787, 1839
BssSI CACGAG 1 cut(s) 187
BssT1I CCWWGG 1 cut(s) 1787
Bst2BI CACGAG 1 cut(s) 187
Bst2UI CCWGG 1 cut(s) 1086
Bst4CI ACNGT 3 cut(s) 661, 709, 1147
Bst6I CTCTTC 2 cut(s) 385, 488
BstC8I GCNNGC 3 cut(s) 742, 863, 1101
BstDEI CTNAG 5 cut(s) 100, 971, 1677, 1883, 1926
BstDSI CCRYGG 2 cut(s) 149, 1504
BstF5I GGATG 6 cut(s) 679, 931, 1345, 1614, 1674, 1942
BstFNI CGCG 2 cut(s) 163, 865
BstHHI GCGC 3 cut(s) 165, 867, 1630
BstMAI GTCTC 7 cut(s) 103, 103, 234, 433, 457, 1440, 1981
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 7 cut(s) 160, 247, 495, 1100, 1105, 1247, 1312
BstNI CCWGG 1 cut(s) 1086
BstNSI RCATGY 1 cut(s) 737
BstSCI CCNGG 7 cut(s) 112, 113, 219, 313, 1084, 1420, 1839
BstSFI CTRYAG 2 cut(s) 1290, 1863
BstUI CGCG 2 cut(s) 163, 865
BstV1I GCAGC 3 cut(s) 162, 496, 1668
BstV2I GAAGAC 2 cut(s) 336, 1538
BstX2I RGATCY 2 cut(s) 199, 883
BstYI RGATCY 2 cut(s) 199, 883
Bsu36I CCTNAGG 1 cut(s) 1677
BsuRI GGCC 7 cut(s) 329, 362, 397, 405, 1241, 1807, 1918
BtgI CCRYGG 2 cut(s) 149, 1504
BtgZI GCGATG 1 cut(s) 222
BtsCI GGATG 6 cut(s) 679, 931, 1345, 1614, 1674, 1942
BtsI GCAGTG 3 cut(s) 139, 780, 1101
BtsIMutI CAGTG 6 cut(s) 139, 714, 780, 841, 876, 1101
Cac8I GCNNGC 3 cut(s) 742, 863, 1101
CciI TCATGA 1 cut(s) 900
CfoI GCGC 3 cut(s) 165, 867, 1630
Cfr10I RCCGGY 1 cut(s) 529
Cfr13I GGNCC 8 cut(s) 217, 328, 361, 395, 404, 1239, 1805, 2032
Cfr9I CCCGGG 1 cut(s) 113
CseI GACGC 2 cut(s) 439, 487
Csp6I GTAC 5 cut(s) 1197, 1335, 1363, 1755, 1952
CspCI CAANNNNNGTGG 4 cut(s) 396, 431, 690, 725
CviAII CATG 5 cut(s) 333, 372, 734, 901, 1523
CviQI GTAC 5 cut(s) 1197, 1335, 1363, 1755, 1952
DdeI CTNAG 5 cut(s) 100, 971, 1677, 1883, 1926
Eam1104I CTCTTC 2 cut(s) 385, 488
EarI CTCTTC 2 cut(s) 385, 488
Eco130I CCWWGG 1 cut(s) 1787
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 3 cut(s) 103, 433, 1440
Eco47I GGWCC 2 cut(s) 217, 2032
Eco57I CTGAAG 2 cut(s) 457, 1006
Eco81I CCTNAGG 1 cut(s) 1677
Eco88I CYCGRG 3 cut(s) 113, 194, 1989
EcoO109I RGGNCCY 2 cut(s) 217, 1239
EcoRI GAATTC 1 cut(s) 1877
EcoRII CCWGG 1 cut(s) 1084
EcoT14I CCWWGG 1 cut(s) 1787
EcoT22I ATGCAT 2 cut(s) 1128, 1579
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 1787
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 5 cut(s) 336, 375, 737, 904, 1526
FaqI GGGAC 2 cut(s) 203, 960
FatI CATG 5 cut(s) 332, 371, 733, 900, 1522
FauI CCCGC 4 cut(s) 569, 870, 1499, 1915
FbaI TGATCA 3 cut(s) 1387, 1939, 1966
Fnu4HI GCNGC 8 cut(s) 130, 155, 176, 419, 510, 1092, 1109, 1657
FokI GGATG 6 cut(s) 666, 938, 1332, 1621, 1681, 1929
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 8 cut(s) 130, 155, 176, 419, 510, 1092, 1109, 1657
FspAI RTGCGCAY 1 cut(s) 1629
FspBI CTAG 2 cut(s) 633, 642
FspI TGCGCA 1 cut(s) 1629
GlaI GCGC 3 cut(s) 164, 866, 1629
GluI GCNGC 8 cut(s) 130, 155, 176, 419, 510, 1092, 1109, 1657
GsaI CCCAGC 1 cut(s) 396
GsuI CTGGAG 1 cut(s) 1107
HaeIII GGCC 7 cut(s) 329, 362, 397, 405, 1241, 1807, 1918
HapII CCGG 9 cut(s) 114, 221, 315, 425, 436, 530, 782, 1422, 1841
HgaI GACGC 2 cut(s) 439, 487
HhaI GCGC 3 cut(s) 165, 867, 1630
Hin1II CATG 5 cut(s) 336, 375, 737, 904, 1526
Hin6I GCGC 3 cut(s) 163, 865, 1628
HinP1I GCGC 3 cut(s) 163, 865, 1628
HincII GTYRAC 1 cut(s) 142
HindII GTYRAC 1 cut(s) 142
HinfI GANTC 6 cut(s) 206, 290, 323, 356, 904, 1469
HpaI GTTAAC 1 cut(s) 142
HpaII CCGG 9 cut(s) 114, 221, 315, 425, 436, 530, 782, 1422, 1841
HphI GGTGA 8 cut(s) 54, 365, 519, 771, 945, 1058, 1409, 1721
Hpy166II GTNNAC 4 cut(s) 142, 1016, 1337, 1417
Hpy188I TCNGA 2 cut(s) 1190, 1445
Hpy188III TCNNGA 9 cut(s) 187, 203, 425, 901, 911, 923, 1274, 1676, 1765
Hpy8I GTNNAC 4 cut(s) 142, 1016, 1337, 1417
Hpy99I CGWCG 2 cut(s) 503, 2032
HpyAV CCTTC 7 cut(s) 15, 75, 1168, 1249, 1645, 1796, 1862
HpyCH4III ACNGT 3 cut(s) 661, 709, 1147
HpyCH4IV ACGT 1 cut(s) 799
HpyF10VI GCNNNNNNNGC 7 cut(s) 160, 247, 495, 1100, 1105, 1247, 1312
HpyF3I CTNAG 5 cut(s) 100, 971, 1677, 1883, 1926
HpySE526I ACGT 1 cut(s) 799
Hsp92II CATG 5 cut(s) 336, 375, 737, 904, 1526
HspAI GCGC 3 cut(s) 163, 865, 1628
KflI GGGWCCC 1 cut(s) 217
Kpn2I TCCGGA 1 cut(s) 424
KpnI GGTACC 1 cut(s) 1200
Ksp22I TGATCA 3 cut(s) 1387, 1939, 1966
KspAI GTTAAC 1 cut(s) 142
LguI GCTCTTC 1 cut(s) 488
LmnI GCTCC 9 cut(s) 25, 380, 655, 832, 1088, 1312, 1457, 1678, 1743
Lsp1109I GCAGC 3 cut(s) 162, 496, 1668
LweI GCATC 5 cut(s) 542, 1135, 1354, 1564, 1586
MaeI CTAG 2 cut(s) 633, 642
MaeII ACGT 1 cut(s) 799
MaeIII GTNAC 6 cut(s) 60, 179, 353, 729, 777, 951
MboII GAAGA 8 cut(s) 3, 6, 336, 372, 463, 505, 531, 1543
MfeI CAATTG 1 cut(s) 1307
MflI RGATCY 2 cut(s) 199, 883
MhlI GDGCHC 3 cut(s) 194, 652, 829
MlyI GAGTC 5 cut(s) 200, 299, 332, 350, 898
MmeI TCCRAC 1 cut(s) 854
Mph1103I ATGCAT 2 cut(s) 1128, 1579
MroI TCCGGA 1 cut(s) 424
MroXI GAANNNNTTC 1 cut(s) 1413
MseI TTAA 5 cut(s) 141, 603, 1323, 1551, 2040
MslI CAYNNNNRTG 2 cut(s) 1623, 1766
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 9 cut(s) 114, 221, 315, 425, 436, 530, 782, 1422, 1841
MspR9I CCNGG 7 cut(s) 114, 115, 221, 315, 1086, 1422, 1841
MunI CAATTG 1 cut(s) 1307
Mva1269I GAATGC 2 cut(s) 1630, 2064
MvaI CCWGG 1 cut(s) 1086
MvnI CGCG 2 cut(s) 163, 865
MwoI GCNNNNNNNGC 7 cut(s) 160, 247, 495, 1100, 1105, 1247, 1312
NciI CCSGG 6 cut(s) 114, 115, 221, 315, 1422, 1841
NlaIII CATG 5 cut(s) 336, 375, 737, 904, 1526
NmeAIII GCCGAG 2 cut(s) 338, 581
NmuCI GTSAC 5 cut(s) 60, 353, 729, 777, 951
NsbI TGCGCA 1 cut(s) 1629
NsiI ATGCAT 2 cut(s) 1128, 1579
NspI RCATGY 1 cut(s) 737
PagI TCATGA 1 cut(s) 900
PciI ACATGT 1 cut(s) 733
PciSI GCTCTTC 1 cut(s) 488
PctI GAATGC 2 cut(s) 1630, 2064
PdmI GAANNNNTTC 1 cut(s) 1413
PfeI GAWTC 1 cut(s) 1469
PflFI GACNNNGTC 1 cut(s) 449
PfoI TCCNGGA 2 cut(s) 219, 1084
PkrI GCNGC 8 cut(s) 131, 156, 177, 420, 511, 1093, 1110, 1658
PleI GAGTC 5 cut(s) 200, 298, 331, 350, 898
PpsI GAGTC 5 cut(s) 200, 298, 331, 350, 898
PpuMI RGGWCCY 1 cut(s) 217
PscI ACATGT 1 cut(s) 733
PsiI TTATAA 1 cut(s) 753
Psp5II RGGWCCY 1 cut(s) 217
Psp6I CCWGG 1 cut(s) 1084
PspFI CCCAGC 1 cut(s) 392
PspGI CCWGG 1 cut(s) 1084
PspPI GGNCC 8 cut(s) 217, 328, 361, 395, 404, 1239, 1805, 2032
PspPPI RGGWCCY 1 cut(s) 217
PsrI GAACNNNNNNTAC 2 cut(s) 37, 69
PstI CTGCAG 2 cut(s) 1294, 1867
PsuI RGATCY 2 cut(s) 199, 883
PsyI GACNNNGTC 1 cut(s) 449
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 5 cut(s) 1198, 1336, 1364, 1756, 1953
RsaNI GTAC 5 cut(s) 1197, 1335, 1363, 1755, 1952
RseI CAYNNNNRTG 2 cut(s) 1623, 1766
SapI GCTCTTC 1 cut(s) 488
SaqAI TTAA 5 cut(s) 141, 603, 1323, 1551, 2040
SatI GCNGC 8 cut(s) 130, 155, 176, 419, 510, 1092, 1109, 1657
Sau96I GGNCC 8 cut(s) 217, 328, 361, 395, 404, 1239, 1805, 2032
ScaI AGTACT 1 cut(s) 1364
SchI GAGTC 5 cut(s) 200, 299, 332, 350, 898
ScrFI CCNGG 7 cut(s) 114, 115, 221, 315, 1086, 1422, 1841
SduI GDGCHC 3 cut(s) 194, 652, 829
SfaNI GCATC 5 cut(s) 542, 1135, 1354, 1564, 1586
SfcI CTRYAG 2 cut(s) 1290, 1863
SgrAI CRCCGGYG 1 cut(s) 529
SinI GGWCC 2 cut(s) 217, 2032
SmaI CCCGGG 1 cut(s) 115
SmiMI CAYNNNNRTG 2 cut(s) 1623, 1766
SmlI CTYRAG 4 cut(s) 399, 670, 814, 844
SmoI CTYRAG 4 cut(s) 399, 670, 814, 844
SspI AATATT 2 cut(s) 257, 1354
SspMI CTAG 2 cut(s) 633, 642
StyD4I CCNGG 7 cut(s) 112, 113, 219, 313, 1084, 1420, 1839
StyI CCWWGG 1 cut(s) 1787
TaaI ACNGT 3 cut(s) 661, 709, 1147
TaiI ACGT 1 cut(s) 802
TaqI TCGA 6 cut(s) 204, 260, 501, 1009, 1764, 2056
TaqII GACCGA 2 cut(s) 330, 337
TatI WGTACW 1 cut(s) 1362
TauI GCSGC 5 cut(s) 132, 157, 421, 1094, 1111
TfiI GAWTC 1 cut(s) 1469
Tru1I TTAA 5 cut(s) 141, 603, 1323, 1551, 2040
Tru9I TTAA 5 cut(s) 141, 603, 1323, 1551, 2040
TscAI CASTG 6 cut(s) 139, 714, 780, 841, 883, 1101
TseFI GTSAC 5 cut(s) 60, 353, 729, 777, 951
TseI GCWGC 3 cut(s) 175, 509, 1656
Tsp45I GTSAC 5 cut(s) 60, 353, 729, 777, 951
TspDTI ATGAA 4 cut(s) 889, 1172, 1268, 1623
TspGWI ACGGA 5 cut(s) 270, 1322, 1462, 1493, 2045
TspMI CCCGGG 1 cut(s) 113
TspRI CASTG 6 cut(s) 139, 714, 780, 841, 883, 1101
Tth111I GACNNNGTC 1 cut(s) 449
VpaK11BI GGWCC 2 cut(s) 217, 2032
XapI RAATTY 6 cut(s) 301, 568, 1561, 1796, 1877, 1957
XceI RCATGY 1 cut(s) 737
XcmI CCANNNNNNNNNTGG 1 cut(s) 1502
XmaI CCCGGG 1 cut(s) 113
XmnI GAANNNNTTC 1 cut(s) 1413
XspI CTAG 2 cut(s) 633, 642
ZrmI AGTACT 1 cut(s) 1364
Zsp2I ATGCAT 2 cut(s) 1128, 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.