Rh2DG179200

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001271
Physical Location & Seq
Forward (+)
15778172 .. 15781674
3503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG179200.1

Sequence Viewer

Length: 1350 bp
ATGCTAGCCACAACCATGCGTTGGTTGCCTAGCTTTGCTTCCTCATCATCATCTGCTTCGTCTTCGTCGTCGTCGTCGCCGAAAAAGAAGCCCAGGGAGGACGGCGGGTGGATTTTCCGGTCGAGGAGGAAGGCCGCGCAGTCGAGGAAACTGCTCAATTTGGGGAGAGACAATGACGGTGGAGCTATAACCAAATGCTCCAGCGCGCCGGGGTCGGAATCCGCGCTGGCCAGTCCGCAGCCGCTTCCTTTGCCGGAATCGGCCATCCGCCGAGAGACGAGGTGTTCTCGCTGTAAGTCATGGGGACATGAGACCTGCTTCCCCTCCCCGGCTCCCAGTGTGTTTTCTTGGCGAGACACTCCAAAGAATAACGTTCAAGTACGTGAGACAGTGTCGTCTAGGAGGAAGAATCAAGATCGAAATCGTTTACAGATCCCTGAACATGACTTCAGGCTCGATGTTCCTGTTAGAAGTGCACCCACTAGTCCATGCGCAAGTCCTAGATTCAGCCCACGCAGACCAAGTGCTGCTGATTTGTGGGCTCCGTCCAGAAATCAAGTCTGGTCTGCACCGGAGATGACTACTAATGATATGACTCAACCTCCCCCTGCCTTATCAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTCCCACGGCTAGAAGTCCCCTTCGACCCCCACAAGCATCATCCCCATTGCATCCCCGGTTATCTGTTACCTCTGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGCCTCCCTTCACCTAGTGTACCATCACCACAACCTGCACTCACACCTAAGCCAGAGTCCCAGCCGATTAAAGGGCAATGGCAAAAGGGAAAGCTTATTGGTCGTGGCACCTTTGGCAGTGTTTATGTTGCTACCAATAGGGACATCAAAGGGGCTAATTTGCTTGTGGATTCATGTGGGGTTGTCAAGCTGGCTGACTTTGGAATGGCCAAACATCTTTCAGGACACGTAGGGAATCTTTCCCTAAAGGGAAGTCCATACTGGATGGCTCCAGAGGCTGCAGCTATGTTTAAAGTTATGAAGGATACTCCACCATTTCCAGAAACATTGTCACCTGAGGGTAAGGACTTCCTTAGGTGCTGCTTCCAAAGAAATCCTGCTGAGAGGTCAACTGCTGCAATGCTATTAGAACATCGATTTATAAAAAACTCCCAGCTGCAGGAAGTCCCATCTACGACCTATAATGCCTCTAATGGGATGAATTTCTTGGAAAGACCCCAGACTCCAATTGAACTTGAAAGAAGACTTGATAGTTCACTGATGATTCCCAGCATTGGCATGCAATTAACTACGGGAAAGGCGGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

449

Amino Acids

48.96

Weight (kDa)

9.99

Isoelectric Point (pI)

69.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 300 - 346 2.3e-10 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 300 - 351 8.3e-06 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1181
AasI GACNNNNNNGTC 1 cut(s) 394
Acc16I TGCGCA 1 cut(s) 493
Acc36I ACCTGC 2 cut(s) 323, 802
AccB1I GGYRCC 1 cut(s) 866
AccB7I CCANNNNNTGG 1 cut(s) 21
AccII CGCG 3 cut(s) 137, 206, 224
AciI CCGC 7 cut(s) 105, 135, 222, 236, 242, 268, 1340
AclI AACGTT 1 cut(s) 372
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 3 cut(s) 228, 261, 966
AcsI RAATTY 1 cut(s) 1240
AcuI CTGAAG 2 cut(s) 433, 747
AdeI CACNNNGTG 1 cut(s) 776
AfaI GTAC 2 cut(s) 381, 780
AfiI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1198, 1233, 1313
AflIII ACRYGT 1 cut(s) 985
AgsI TTSAA 3 cut(s) 377, 1271, 1277
AhlI ACTAGT 1 cut(s) 482
AjnI CCWGG 2 cut(s) 92, 751
AluBI AGCT 6 cut(s) 33, 185, 853, 949, 1043, 1195
AluI AGCT 6 cut(s) 33, 185, 853, 949, 1043, 1195
Alw21I GWGCWC 1 cut(s) 478
Alw26I GTCTC 5 cut(s) 162, 269, 305, 348, 380
Alw44I GTGCAC 1 cut(s) 474
AlwI GGATC 1 cut(s) 427
AlwNI CAGNNNCTG 1 cut(s) 1037
AoxI GGCC 4 cut(s) 132, 228, 261, 966
ApaLI GTGCAC 1 cut(s) 474
ApeKI GCWGC 7 cut(s) 238, 527, 1037, 1040, 1119, 1154, 1195
ApoI RAATTY 1 cut(s) 1240
AspLEI GCGC 5 cut(s) 139, 206, 208, 226, 494
AsuC2I CCSGG 3 cut(s) 210, 329, 709
AsuHPI GGTGA 3 cut(s) 762, 777, 1083
AsuNHI GCTAGC 1 cut(s) 4
AxyI CCTNAGG 2 cut(s) 1095, 1112
BaeGI GKGCMC 1 cut(s) 478
BalI TGGCCA 2 cut(s) 230, 968
BanI GGYRCC 1 cut(s) 866
BanII GRGCYC 1 cut(s) 544
BbsI GAAGAC 2 cut(s) 54, 1288
Bbv12I GWGCWC 1 cut(s) 478
BbvI GCAGC 7 cut(s) 250, 514, 1024, 1052, 1106, 1141, 1182
BccI CCATC 5 cut(s) 272, 741, 790, 1018, 1216
BceAI ACGGC 2 cut(s) 118, 675
BcgI CGANNNNNNTGC 2 cut(s) 133, 167
BciT130I CCWGG 2 cut(s) 94, 753
BciVI GTATCC 1 cut(s) 1057
BcnI CCSGG 3 cut(s) 210, 329, 709
BcoDI GTCTC 5 cut(s) 162, 269, 305, 348, 380
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
BfmI CTRYAG 2 cut(s) 1038, 1196
BfuAI ACCTGC 2 cut(s) 323, 802
BfuI GTATCC 1 cut(s) 1057
Bme1390I CCNGG 5 cut(s) 94, 210, 329, 709, 753
BmiI GGNNCC 4 cut(s) 333, 543, 868, 1029
BmrFI CCNGG 5 cut(s) 94, 210, 329, 709, 753
BmrI ACTGGG 1 cut(s) 330
BmsI GCATC 2 cut(s) 698, 712
BmtI GCTAGC 1 cut(s) 8
BmuI ACTGGG 1 cut(s) 330
BpiI GAAGAC 2 cut(s) 54, 1288
BplI GAGNNNNNCTC 4 cut(s) 271, 303, 747, 779
BpmI CTGGAG 3 cut(s) 184, 774, 1014
Bpu10I CCTNAGC 1 cut(s) 807
BpuMI CCSGG 3 cut(s) 210, 329, 709
Bsa29I ATCGAT 1 cut(s) 1174
BsaAI YACGTR 2 cut(s) 383, 988
BsaBI GATNNNNATC 1 cut(s) 420
BsaI GGTCTC 1 cut(s) 305
BsaJI CCNNGG 6 cut(s) 92, 93, 209, 327, 657, 707
BsaWI WCCGGW 2 cut(s) 117, 571
BsaXI ACNNNNNCTCC 2 cut(s) 586, 616
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1198, 1233, 1313
Bse1I ACTGG 3 cut(s) 231, 336, 1025
Bse21I CCTNAGG 2 cut(s) 1095, 1112
Bse3DI GCAATG 4 cut(s) 698, 737, 842, 1164
Bse8I GATNNNNATC 1 cut(s) 420
BseBI CCWGG 2 cut(s) 94, 753
BseCI ATCGAT 1 cut(s) 1174
BseDI CCNNGG 6 cut(s) 92, 93, 209, 327, 657, 707
BseGI GGATG 6 cut(s) 264, 692, 703, 733, 1029, 1242
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1198, 1233, 1313
BseMI GCAATG 4 cut(s) 698, 737, 842, 1164
BseMII CTCAG 2 cut(s) 1086, 1131
BseNI ACTGG 3 cut(s) 231, 336, 1025
BsePI GCGCGC 1 cut(s) 204
BseRI GAGGAG 1 cut(s) 139
BseSI GKGCMC 1 cut(s) 478
BseXI GCAGC 7 cut(s) 250, 514, 1024, 1052, 1106, 1141, 1182
BseYI CCCAGC 3 cut(s) 819, 1191, 1307
BsgI GTGCAG 2 cut(s) 552, 780
Bsh1236I CGCG 3 cut(s) 137, 206, 224
Bsh1285I CGRYCG 1 cut(s) 122
BshFI GGCC 4 cut(s) 134, 230, 263, 968
BshNI GGYRCC 1 cut(s) 866
BshVI ATCGAT 1 cut(s) 1174
BsiEI CGRYCG 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 478
BsiSI CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
BslFI GGGAC 6 cut(s) 318, 639, 654, 802, 914, 1190
BslI CCNNNNNNNGG 6 cut(s) 21, 328, 830, 1198, 1233, 1313
BsmAI GTCTC 5 cut(s) 162, 269, 305, 348, 380
BsmBI CGTCTC 1 cut(s) 269
BsmFI GGGAC 6 cut(s) 318, 639, 654, 802, 914, 1190
BsnI GGCC 4 cut(s) 134, 230, 263, 968
Bso31I GGTCTC 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 478, 544
Bsp143I GATC 2 cut(s) 415, 432
BspACI CCGC 7 cut(s) 105, 135, 222, 236, 242, 268, 1340
BspANI GGCC 4 cut(s) 134, 230, 263, 968
BspCNI CTCAG 2 cut(s) 1087, 1132
BspDI ATCGAT 1 cut(s) 1174
BspFNI CGCG 3 cut(s) 137, 206, 224
BspLI GGNNCC 4 cut(s) 333, 543, 868, 1029
BspMAI CTGCAG 2 cut(s) 1042, 1200
BspMI ACCTGC 2 cut(s) 323, 802
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 427
BspT107I GGYRCC 1 cut(s) 866
BspTNI GGTCTC 1 cut(s) 305
BsrDI GCAATG 4 cut(s) 698, 737, 842, 1164
BsrI ACTGG 3 cut(s) 231, 336, 1025
BssECI CCNNGG 6 cut(s) 92, 93, 209, 327, 657, 707
BssHII GCGCGC 1 cut(s) 204
BssMI GATC 2 cut(s) 415, 432
Bst2UI CCWGG 2 cut(s) 94, 753
Bst4CI ACNGT 3 cut(s) 179, 391, 653
BstBAI YACGTR 2 cut(s) 383, 988
BstC8I GCNNGC 6 cut(s) 6, 206, 228, 760, 951, 1319
BstDEI CTNAG 4 cut(s) 807, 1095, 1112, 1140
BstDSI CCRYGG 1 cut(s) 657
BstF5I GGATG 6 cut(s) 264, 692, 703, 733, 1029, 1242
BstFNI CGCG 3 cut(s) 137, 206, 224
BstHHI GCGC 5 cut(s) 139, 206, 208, 226, 494
BstKTI GATC 2 cut(s) 418, 435
BstMAI GTCTC 5 cut(s) 162, 269, 305, 348, 380
BstMBI GATC 2 cut(s) 415, 432
BstMCI CGRYCG 1 cut(s) 122
BstMWI GCNNNNNNNGC 4 cut(s) 25, 250, 873, 1034
BstNI CCWGG 2 cut(s) 94, 753
BstNSI RCATGY 1 cut(s) 1321
BstSCI CCNGG 5 cut(s) 92, 208, 327, 707, 751
BstSFI CTRYAG 2 cut(s) 1038, 1196
BstSLI GKGCMC 1 cut(s) 478
BstUI CGCG 3 cut(s) 137, 206, 224
BstV1I GCAGC 7 cut(s) 250, 514, 1024, 1052, 1106, 1141, 1182
BstV2I GAAGAC 2 cut(s) 54, 1288
BstX2I RGATCY 1 cut(s) 432
BstYI RGATCY 1 cut(s) 432
Bsu15I ATCGAT 1 cut(s) 1174
Bsu36I CCTNAGG 2 cut(s) 1095, 1112
BsuI GTATCC 1 cut(s) 1057
BsuRI GGCC 4 cut(s) 134, 230, 263, 968
BsuTUI ATCGAT 1 cut(s) 1174
BtgI CCRYGG 1 cut(s) 657
BtsCI GGATG 6 cut(s) 264, 692, 703, 733, 1029, 1242
BtsI GCAGTG 1 cut(s) 883
BtsIMutI CAGTG 4 cut(s) 343, 396, 883, 1295
BveI ACCTGC 2 cut(s) 323, 802
Cac8I GCNNGC 6 cut(s) 6, 206, 228, 760, 951, 1319
CaiI CAGNNNCTG 1 cut(s) 1037
CfoI GCGC 5 cut(s) 139, 206, 208, 226, 494
ClaI ATCGAT 1 cut(s) 1174
Csp6I GTAC 2 cut(s) 380, 779
CspCI CAANNNNNGTGG 2 cut(s) 160, 195
CviAII CATG 7 cut(s) 16, 300, 308, 443, 489, 933, 1318
CviQI GTAC 2 cut(s) 380, 779
DdeI CTNAG 4 cut(s) 807, 1095, 1112, 1140
DpnI GATC 2 cut(s) 417, 434
DpnII GATC 2 cut(s) 415, 432
DraI TTTAAA 1 cut(s) 1051
DraIII CACNNNGTG 1 cut(s) 776
DrdI GACNNNNNNGTC 1 cut(s) 394
DseDI GACNNNNNNGTC 1 cut(s) 394
EaeI YGGCCR 3 cut(s) 228, 261, 966
EciI GGCGGA 1 cut(s) 257
Eco24I GRGCYC 1 cut(s) 544
Eco31I GGTCTC 1 cut(s) 305
Eco57I CTGAAG 2 cut(s) 433, 747
Eco81I CCTNAGG 2 cut(s) 1095, 1112
EcoRII CCWGG 2 cut(s) 92, 751
EcoT38I GRGCYC 1 cut(s) 544
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 7 cut(s) 19, 303, 311, 446, 492, 936, 1321
FalI AAGNNNNNCTT 2 cut(s) 751, 783
FaqI GGGAC 6 cut(s) 318, 639, 654, 802, 914, 1190
FatI CATG 7 cut(s) 15, 299, 307, 442, 488, 932, 1317
FauI CCCGC 1 cut(s) 98
FokI GGATG 6 cut(s) 251, 679, 690, 720, 1036, 1249
FriOI GRGCYC 1 cut(s) 544
FspBI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
FspI TGCGCA 1 cut(s) 493
GlaI GCGC 5 cut(s) 138, 205, 207, 225, 493
GsaI CCCAGC 3 cut(s) 823, 1195, 1311
GsuI CTGGAG 3 cut(s) 184, 774, 1014
HaeIII GGCC 4 cut(s) 134, 230, 263, 968
HapII CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
HhaI GCGC 5 cut(s) 139, 206, 208, 226, 494
Hin1II CATG 7 cut(s) 19, 303, 311, 446, 492, 936, 1321
Hin6I GCGC 5 cut(s) 137, 204, 206, 224, 492
HinP1I GCGC 5 cut(s) 137, 204, 206, 224, 492
HincII GTYRAC 1 cut(s) 1149
HindII GTYRAC 1 cut(s) 1149
HindIII AAGCTT 1 cut(s) 851
HpaII CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
HphI GGTGA 3 cut(s) 762, 777, 1083
Hpy166II GTNNAC 5 cut(s) 428, 476, 779, 1149, 1295
Hpy188I TCNGA 4 cut(s) 217, 619, 727, 1349
Hpy188III TCNNGA 5 cut(s) 413, 549, 981, 1031, 1079
Hpy8I GTNNAC 5 cut(s) 428, 476, 779, 1149, 1295
Hpy99I CGWCG 4 cut(s) 70, 73, 76, 79
HpyAV CCTTC 4 cut(s) 124, 683, 777, 1054
HpyCH4III ACNGT 3 cut(s) 179, 391, 653
HpyCH4IV ACGT 3 cut(s) 372, 382, 987
HpyCH4V TGCA 8 cut(s) 476, 569, 703, 797, 1040, 1157, 1198, 1321
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 250, 873, 1034
HpyF3I CTNAG 4 cut(s) 807, 1095, 1112, 1140
HpySE526I ACGT 3 cut(s) 372, 382, 987
Hsp92II CATG 7 cut(s) 19, 303, 311, 446, 492, 936, 1321
HspAI GCGC 5 cut(s) 137, 204, 206, 224, 492
Kzo9I GATC 2 cut(s) 415, 432
LmnI GCTCC 6 cut(s) 182, 203, 337, 547, 755, 1033
Lsp1109I GCAGC 7 cut(s) 250, 514, 1024, 1052, 1106, 1141, 1182
LweI GCATC 2 cut(s) 698, 712
MaeI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
MaeII ACGT 3 cut(s) 372, 382, 987
MaeIII GTNAC 2 cut(s) 718, 1089
MalI GATC 2 cut(s) 417, 434
MboI GATC 2 cut(s) 415, 432
MboII GAAGA 3 cut(s) 54, 418, 1293
MfeI CAATTG 1 cut(s) 1266
MflI RGATCY 1 cut(s) 432
MhlI GDGCHC 2 cut(s) 478, 544
MlsI TGGCCA 2 cut(s) 230, 968
MluCI AATT 5 cut(s) 157, 916, 1240, 1266, 1322
MluNI TGGCCA 2 cut(s) 230, 968
MlyI GAGTC 3 cut(s) 589, 824, 1255
MmeI TCCRAC 1 cut(s) 195
Mox20I TGGCCA 2 cut(s) 230, 968
MscI TGGCCA 2 cut(s) 230, 968
MseI TTAA 3 cut(s) 828, 1050, 1325
MslI CAYNNNNRTG 2 cut(s) 14, 1316
Msp20I TGGCCA 2 cut(s) 230, 968
MspA1I CMGCKG 1 cut(s) 1195
MspI CCGG 6 cut(s) 118, 209, 254, 329, 572, 709
MspR9I CCNGG 5 cut(s) 94, 210, 329, 709, 753
MunI CAATTG 1 cut(s) 1266
MvaI CCWGG 2 cut(s) 94, 753
MvnI CGCG 3 cut(s) 137, 206, 224
MwoI GCNNNNNNNGC 4 cut(s) 25, 250, 873, 1034
NciI CCSGG 3 cut(s) 210, 329, 709
NdeII GATC 2 cut(s) 415, 432
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 7 cut(s) 19, 303, 311, 446, 492, 936, 1321
NlaIV GGNNCC 4 cut(s) 333, 543, 868, 1029
NmeAIII GCCGAG 1 cut(s) 296
NmuCI GTSAC 1 cut(s) 1089
NsbI TGCGCA 1 cut(s) 493
NspI RCATGY 1 cut(s) 1321
PaeI GCATGC 1 cut(s) 1321
PasI CCCWGGG 1 cut(s) 93
PauI GCGCGC 1 cut(s) 204
PcsI WCGNNNNNNNCGW 3 cut(s) 65, 71, 77
PfeI GAWTC 7 cut(s) 218, 257, 409, 504, 929, 994, 1303
PflFI GACNNNGTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 751
PleI GAGTC 3 cut(s) 589, 823, 1255
PpsI GAGTC 3 cut(s) 589, 823, 1255
Ppu21I YACGTR 2 cut(s) 383, 988
PsiI TTATAA 1 cut(s) 1181
Psp1406I AACGTT 1 cut(s) 372
Psp6I CCWGG 2 cut(s) 92, 751
PspFI CCCAGC 3 cut(s) 819, 1191, 1307
PspGI CCWGG 2 cut(s) 92, 751
PspN4I GGNNCC 4 cut(s) 333, 543, 868, 1029
PsrI GAACNNNNNNTAC 2 cut(s) 622, 654
PstI CTGCAG 2 cut(s) 1042, 1200
PstNI CAGNNNCTG 1 cut(s) 1037
PsuI RGATCY 1 cut(s) 432
PsyI GACNNNGTC 1 cut(s) 391
PteI GCGCGC 1 cut(s) 204
PvuII CAGCTG 1 cut(s) 1195
RsaI GTAC 2 cut(s) 381, 780
RsaNI GTAC 2 cut(s) 380, 779
RseI CAYNNNNRTG 2 cut(s) 14, 1316
SaqAI TTAA 3 cut(s) 828, 1050, 1325
Sau3AI GATC 2 cut(s) 415, 432
SchI GAGTC 3 cut(s) 589, 824, 1255
ScrFI CCNGG 5 cut(s) 94, 210, 329, 709, 753
SduI GDGCHC 2 cut(s) 478, 544
SfaNI GCATC 2 cut(s) 698, 712
SfcI CTRYAG 2 cut(s) 1038, 1196
SmiMI CAYNNNNRTG 2 cut(s) 14, 1316
SpeI ACTAGT 1 cut(s) 482
SphI GCATGC 1 cut(s) 1321
Sse9I AATT 5 cut(s) 157, 916, 1240, 1266, 1322
SsiI CCGC 7 cut(s) 105, 135, 222, 236, 242, 268, 1340
SspMI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
StyD4I CCNGG 5 cut(s) 92, 208, 327, 707, 751
TaaI ACNGT 3 cut(s) 179, 391, 653
TaiI ACGT 3 cut(s) 375, 385, 990
TaqI TCGA 6 cut(s) 122, 143, 418, 456, 676, 1174
TasI AATT 5 cut(s) 157, 916, 1240, 1266, 1322
TauI GCSGC 3 cut(s) 137, 244, 1343
TfiI GAWTC 7 cut(s) 218, 257, 409, 504, 929, 994, 1303
Tru1I TTAA 3 cut(s) 828, 1050, 1325
Tru9I TTAA 3 cut(s) 828, 1050, 1325
TscAI CASTG 4 cut(s) 343, 396, 883, 1302
TseFI GTSAC 1 cut(s) 1089
TseI GCWGC 7 cut(s) 238, 527, 1037, 1040, 1119, 1154, 1195
Tsp45I GTSAC 1 cut(s) 1089
TspDTI ATGAA 4 cut(s) 648, 921, 1073, 1253
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 4 cut(s) 343, 396, 883, 1302
Tth111I GACNNNGTC 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 21
VneI GTGCAC 1 cut(s) 474
XapI RAATTY 1 cut(s) 1240
XceI RCATGY 1 cut(s) 1321
XspI CTAG 7 cut(s) 5, 30, 399, 483, 501, 663, 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.