Rh2CG178200

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001271
Physical Location & Seq
Forward (+)
16352468 .. 16355946
3479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG178200.1

Sequence Viewer

Length: 1455 bp
ATGCTAGCCACAACCATGCGTTGGTTGCCTAGCTTTGCTTCCTCATCATCATCTGCTTCGTCTTCGTCGCCAAAAAAGAAGGCCAGGGAGGACGGCGGGTGGATTTTCCGGTCGAGGAGGAAGGCCGCGCAGTCGAGGAAACTGCTCAATTTGGGGAGAGACAATGACGGTGGAGCTATAACCAAATGCTCCAGCGCGCCGGGGTCGGAATCCGCGCTGGCCAGTCCGCAGCCGCTTCCTTTGCCGGAATCGGCCATCCGCCGAGAGACGAGGTGTTCTCGCTGTAAGTCATGGGGACATGAGAACTGCTTCCTCTCCCCGGCTCCCAGTGTGTTTTCTTGGCGAGACACTCCAAAGAATAACGTTCGAGTACGTGAGACAGTGTCGTCTAGGAGGAAGAATCAAGATCGAAATCGTTTACAGATCCCTGAACATGACTTCAGGCTCGATGTTCCTGTTAGAAGTGCACCCACTAGTCCATGCGCAAGTCCTAGATTCAGCCCACGCAGACCAAGTGCTGCTGATTTGTGGGCTCCGTCCAGAAATCAAGTCTGGTCTGCACCGGAGATGACTACTAATGATATGACTCAACCTCCCCCTGCCTCATCAGATGTATTTAGTAATGAAAGTTCCCCTATAAACAGTCCCACGGCTAGAAGTCCCCTTCGACCCCCACAAGCATCATCCCCATTGCATCCCCGGCTAGAAGTCCATCCATTGCCTCTGCCTCCTGGAGCAAGCCTTCCTTCACCTAGTGTACCATCACCACAACCTCCACTCACACCTAAGCCAGAGCCCCAGCCGATTAAAGGGCAATGGCAAAAGGGAAAGCTTATTGGTCGTGGCACCTTTGGCAGTGTTTATGTTGCTACCAATAGGGACATCAAAGGGGCTAATTTGCTTGTGGATTCATGTGGGGTTGTCAAGCTAGCTGACTTTGGAATGGCCAAACATCTTTCAGGACATGTAGGGAATCTTTCCCTAAAGGGAAGTCCATACTGGATGGCTCCAGAGCTGATGCAATCTGAGATGCATAAAGATAACAGCTCTGATTTGGCTCTTGCTGTTGATATATGGAGTTTGGGTTGTACTATAATTGAAATGTTCACAGGAAAACCTCCATGGAGTGAGTATGAAGGGGCTGCAGCTATGTTTAAAGTTATGAAGGATACTCCACCATTTCCAGAAACATTGTCACCTGAGGGTAAGGACTTCCTTAGGTGCTGCTTCCAAAGAAATCCTGCTGAGAGGTCAACTGCTGCAATGCTATTAGAACATCGATTTATAAAAAACTCCCAGCTGCAGGAAGTCCCATCTACGACCTATAATGCCTCTAATGGGATGAATTTCTTGGAAAGACCCCAGACTCCAATTGAACTTGAAAGAAGACTTGATAGTTCATTGATGATTCCCAGCATTGGCATGCAATTAACTACGGGAAAGGCGGCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

484

Amino Acids

53.08

Weight (kDa)

9.64

Isoelectric Point (pI)

68.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 293 - 429 8.6e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 293 - 425 1.4e-17 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1286
AasI GACNNNNNNGTC 1 cut(s) 385
Acc16I TGCGCA 1 cut(s) 484
AccB1I GGYRCC 1 cut(s) 845
AccB7I CCANNNNNTGG 1 cut(s) 21
AccII CGCG 3 cut(s) 128, 197, 215
AciI CCGC 7 cut(s) 96, 126, 213, 227, 233, 259, 1445
AclI AACGTT 1 cut(s) 363
AclWI GGATC 1 cut(s) 418
AcoI YGGCCR 3 cut(s) 219, 252, 945
AcsI RAATTY 1 cut(s) 1345
AcuI CTGAAG 1 cut(s) 424
AdeI CACNNNGTG 1 cut(s) 755
AfaI GTAC 3 cut(s) 372, 759, 1090
AfiI CCNNNNNNNGG 6 cut(s) 21, 319, 809, 1303, 1338, 1418
AflIII ACRYGT 1 cut(s) 964
AgsI TTSAA 3 cut(s) 1100, 1376, 1382
AhlI ACTAGT 1 cut(s) 473
AjnI CCWGG 2 cut(s) 83, 730
AluBI AGCT 9 cut(s) 33, 176, 832, 928, 932, 1015, 1047, 1148, 1300
AluI AGCT 9 cut(s) 33, 176, 832, 928, 932, 1015, 1047, 1148, 1300
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 4 cut(s) 153, 260, 339, 371
Alw44I GTGCAC 1 cut(s) 465
AlwI GGATC 1 cut(s) 418
AoxI GGCC 5 cut(s) 81, 123, 219, 252, 945
ApaLI GTGCAC 1 cut(s) 465
ApeKI GCWGC 7 cut(s) 229, 518, 1142, 1145, 1224, 1259, 1300
ApoI RAATTY 1 cut(s) 1345
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 5 cut(s) 130, 197, 199, 217, 485
AsuC2I CCSGG 3 cut(s) 201, 320, 700
AsuHPI GGTGA 3 cut(s) 741, 756, 1188
AsuNHI GCTAGC 2 cut(s) 4, 928
AxyI CCTNAGG 2 cut(s) 1200, 1217
BaeGI GKGCMC 1 cut(s) 469
BalI TGGCCA 2 cut(s) 221, 947
BanI GGYRCC 1 cut(s) 845
BanII GRGCYC 2 cut(s) 535, 798
BbsI GAAGAC 2 cut(s) 54, 1393
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 7 cut(s) 241, 505, 1129, 1157, 1211, 1246, 1287
BccI CCATC 5 cut(s) 263, 720, 769, 997, 1321
BceAI ACGGC 2 cut(s) 109, 666
BcgI CGANNNNNNTGC 2 cut(s) 124, 158
BciT130I CCWGG 2 cut(s) 85, 732
BciVI GTATCC 1 cut(s) 1162
BcnI CCSGG 3 cut(s) 201, 320, 700
BcoDI GTCTC 4 cut(s) 153, 260, 339, 371
BcuI ACTAGT 1 cut(s) 473
BfaI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 929
BfmI CTRYAG 2 cut(s) 1143, 1301
BfuI GTATCC 1 cut(s) 1162
Bme1390I CCNGG 5 cut(s) 85, 201, 320, 700, 732
BmiI GGNNCC 4 cut(s) 324, 534, 847, 1008
BmrFI CCNGG 5 cut(s) 85, 201, 320, 700, 732
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 4 cut(s) 689, 703, 1008, 1020
BmtI GCTAGC 2 cut(s) 8, 932
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 54, 1393
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpmI CTGGAG 3 cut(s) 175, 753, 993
Bpu10I CCTNAGC 1 cut(s) 786
BpuMI CCSGG 3 cut(s) 201, 320, 700
Bsa29I ATCGAT 1 cut(s) 1279
BsaAI YACGTR 1 cut(s) 374
BsaBI GATNNNNATC 1 cut(s) 411
BsaJI CCNNGG 6 cut(s) 84, 200, 318, 648, 698, 1121
BsaWI WCCGGW 2 cut(s) 108, 562
BsaXI ACNNNNNCTCC 4 cut(s) 577, 607, 1069, 1099
Bsc4I CCNNNNNNNGG 6 cut(s) 21, 319, 809, 1303, 1338, 1418
Bse1I ACTGG 3 cut(s) 222, 327, 1004
Bse21I CCTNAGG 2 cut(s) 1200, 1217
Bse3DI GCAATG 4 cut(s) 689, 716, 821, 1269
Bse8I GATNNNNATC 1 cut(s) 411
BseBI CCWGG 2 cut(s) 85, 732
BseCI ATCGAT 1 cut(s) 1279
BseDI CCNNGG 6 cut(s) 84, 200, 318, 648, 698, 1121
BseGI GGATG 6 cut(s) 255, 683, 694, 712, 1008, 1347
BseJI GATNNNNATC 1 cut(s) 411
BseLI CCNNNNNNNGG 6 cut(s) 21, 319, 809, 1303, 1338, 1418
BseMI GCAATG 4 cut(s) 689, 716, 821, 1269
BseMII CTCAG 3 cut(s) 1017, 1191, 1236
BseNI ACTGG 3 cut(s) 222, 327, 1004
BsePI GCGCGC 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 130
BseSI GKGCMC 1 cut(s) 469
BseXI GCAGC 7 cut(s) 241, 505, 1129, 1157, 1211, 1246, 1287
BseYI CCCAGC 3 cut(s) 798, 1296, 1412
BsgI GTGCAG 1 cut(s) 543
Bsh1236I CGCG 3 cut(s) 128, 197, 215
Bsh1285I CGRYCG 1 cut(s) 113
BshFI GGCC 5 cut(s) 83, 125, 221, 254, 947
BshNI GGYRCC 1 cut(s) 845
BshVI ATCGAT 1 cut(s) 1279
BsiEI CGRYCG 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 469
BsiSI CCGG 6 cut(s) 109, 200, 245, 320, 563, 700
BslFI GGGAC 5 cut(s) 309, 630, 645, 893, 1295
BslI CCNNNNNNNGG 6 cut(s) 21, 319, 809, 1303, 1338, 1418
BsmAI GTCTC 4 cut(s) 153, 260, 339, 371
BsmBI CGTCTC 1 cut(s) 260
BsmFI GGGAC 5 cut(s) 309, 630, 645, 893, 1295
BsnI GGCC 5 cut(s) 83, 125, 221, 254, 947
Bsp1286I GDGCHC 3 cut(s) 469, 535, 798
Bsp143I GATC 2 cut(s) 406, 423
Bsp19I CCATGG 1 cut(s) 1121
BspACI CCGC 7 cut(s) 96, 126, 213, 227, 233, 259, 1445
BspANI GGCC 5 cut(s) 83, 125, 221, 254, 947
BspCNI CTCAG 3 cut(s) 1018, 1192, 1237
BspDI ATCGAT 1 cut(s) 1279
BspFNI CGCG 3 cut(s) 128, 197, 215
BspLI GGNNCC 4 cut(s) 324, 534, 847, 1008
BspMAI CTGCAG 2 cut(s) 1147, 1305
BspOI GCTAGC 2 cut(s) 8, 932
BspPI GGATC 1 cut(s) 418
BspT107I GGYRCC 1 cut(s) 845
BsrDI GCAATG 4 cut(s) 689, 716, 821, 1269
BsrI ACTGG 3 cut(s) 222, 327, 1004
BssECI CCNNGG 6 cut(s) 84, 200, 318, 648, 698, 1121
BssHII GCGCGC 1 cut(s) 195
BssMI GATC 2 cut(s) 406, 423
BssT1I CCWWGG 1 cut(s) 1121
Bst2UI CCWGG 2 cut(s) 85, 732
Bst4CI ACNGT 3 cut(s) 170, 382, 644
BstBAI YACGTR 1 cut(s) 374
BstC8I GCNNGC 6 cut(s) 6, 197, 219, 739, 930, 1424
BstDEI CTNAG 5 cut(s) 786, 1026, 1200, 1217, 1245
BstDSI CCRYGG 2 cut(s) 648, 1121
BstF5I GGATG 6 cut(s) 255, 683, 694, 712, 1008, 1347
BstFNI CGCG 3 cut(s) 128, 197, 215
BstHHI GCGC 5 cut(s) 130, 197, 199, 217, 485
BstKTI GATC 2 cut(s) 409, 426
BstMAI GTCTC 4 cut(s) 153, 260, 339, 371
BstMBI GATC 2 cut(s) 406, 423
BstMCI CGRYCG 1 cut(s) 113
BstMWI GCNNNNNNNGC 4 cut(s) 25, 241, 700, 852
BstNI CCWGG 2 cut(s) 85, 732
BstNSI RCATGY 2 cut(s) 968, 1426
BstSCI CCNGG 5 cut(s) 83, 199, 318, 698, 730
BstSFI CTRYAG 2 cut(s) 1143, 1301
BstSLI GKGCMC 1 cut(s) 469
BstUI CGCG 3 cut(s) 128, 197, 215
BstV1I GCAGC 7 cut(s) 241, 505, 1129, 1157, 1211, 1246, 1287
BstV2I GAAGAC 2 cut(s) 54, 1393
BstX2I RGATCY 1 cut(s) 423
BstYI RGATCY 1 cut(s) 423
Bsu15I ATCGAT 1 cut(s) 1279
Bsu36I CCTNAGG 2 cut(s) 1200, 1217
BsuI GTATCC 1 cut(s) 1162
BsuRI GGCC 5 cut(s) 83, 125, 221, 254, 947
BsuTUI ATCGAT 1 cut(s) 1279
BtgI CCRYGG 2 cut(s) 648, 1121
BtsCI GGATG 6 cut(s) 255, 683, 694, 712, 1008, 1347
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 3 cut(s) 334, 387, 862
Cac8I GCNNGC 6 cut(s) 6, 197, 219, 739, 930, 1424
CfoI GCGC 5 cut(s) 130, 197, 199, 217, 485
ClaI ATCGAT 1 cut(s) 1279
Csp6I GTAC 3 cut(s) 371, 758, 1089
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 9 cut(s) 16, 291, 299, 434, 480, 912, 965, 1122, 1423
CviQI GTAC 3 cut(s) 371, 758, 1089
DdeI CTNAG 5 cut(s) 786, 1026, 1200, 1217, 1245
DpnI GATC 2 cut(s) 408, 425
DpnII GATC 2 cut(s) 406, 423
DraI TTTAAA 1 cut(s) 1156
DraIII CACNNNGTG 1 cut(s) 755
DrdI GACNNNNNNGTC 1 cut(s) 385
DseDI GACNNNNNNGTC 1 cut(s) 385
EaeI YGGCCR 3 cut(s) 219, 252, 945
EciI GGCGGA 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 1121
Eco24I GRGCYC 2 cut(s) 535, 798
Eco57I CTGAAG 1 cut(s) 424
Eco81I CCTNAGG 2 cut(s) 1200, 1217
EcoRII CCWGG 2 cut(s) 83, 730
EcoT14I CCWWGG 1 cut(s) 1121
EcoT22I ATGCAT 1 cut(s) 1035
EcoT38I GRGCYC 2 cut(s) 535, 798
ErhI CCWWGG 1 cut(s) 1121
Esp3I CGTCTC 1 cut(s) 260
FaeI CATG 9 cut(s) 19, 294, 302, 437, 483, 915, 968, 1125, 1426
FalI AAGNNNNNCTT 2 cut(s) 730, 762
FaqI GGGAC 5 cut(s) 309, 630, 645, 893, 1295
FatI CATG 9 cut(s) 15, 290, 298, 433, 479, 911, 964, 1121, 1422
FauI CCCGC 1 cut(s) 89
FokI GGATG 6 cut(s) 242, 670, 681, 699, 1015, 1354
FriOI GRGCYC 2 cut(s) 535, 798
FspBI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 929
FspI TGCGCA 1 cut(s) 484
GlaI GCGC 5 cut(s) 129, 196, 198, 216, 484
GsaI CCCAGC 3 cut(s) 802, 1300, 1416
GsuI CTGGAG 3 cut(s) 175, 753, 993
HaeIII GGCC 5 cut(s) 83, 125, 221, 254, 947
HapII CCGG 6 cut(s) 109, 200, 245, 320, 563, 700
HhaI GCGC 5 cut(s) 130, 197, 199, 217, 485
Hin1II CATG 9 cut(s) 19, 294, 302, 437, 483, 915, 968, 1125, 1426
Hin6I GCGC 5 cut(s) 128, 195, 197, 215, 483
HinP1I GCGC 5 cut(s) 128, 195, 197, 215, 483
HincII GTYRAC 1 cut(s) 1254
HindII GTYRAC 1 cut(s) 1254
HindIII AAGCTT 1 cut(s) 830
HinfI GANTC 9 cut(s) 209, 248, 400, 495, 586, 908, 973, 1366, 1408
HpaII CCGG 6 cut(s) 109, 200, 245, 320, 563, 700
HphI GGTGA 3 cut(s) 741, 756, 1188
Hpy166II GTNNAC 5 cut(s) 419, 467, 758, 1107, 1254
Hpy188I TCNGA 5 cut(s) 208, 610, 1027, 1051, 1454
Hpy188III TCNNGA 5 cut(s) 404, 540, 960, 1010, 1184
Hpy8I GTNNAC 5 cut(s) 419, 467, 758, 1107, 1254
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 7 cut(s) 73, 115, 674, 752, 756, 1130, 1159
HpyCH4III ACNGT 3 cut(s) 170, 382, 644
HpyCH4IV ACGT 2 cut(s) 363, 373
HpyCH4V TGCA 9 cut(s) 467, 560, 694, 1021, 1033, 1145, 1262, 1303, 1426
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 241, 700, 852
HpyF3I CTNAG 5 cut(s) 786, 1026, 1200, 1217, 1245
HpySE526I ACGT 2 cut(s) 363, 373
Hsp92II CATG 9 cut(s) 19, 294, 302, 437, 483, 915, 968, 1125, 1426
HspAI GCGC 5 cut(s) 128, 195, 197, 215, 483
Kzo9I GATC 2 cut(s) 406, 423
LmnI GCTCC 6 cut(s) 173, 194, 328, 538, 734, 1012
Lsp1109I GCAGC 7 cut(s) 241, 505, 1129, 1157, 1211, 1246, 1287
LweI GCATC 4 cut(s) 689, 703, 1008, 1020
MaeI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 929
MaeII ACGT 2 cut(s) 363, 373
MaeIII GTNAC 1 cut(s) 1194
MalI GATC 2 cut(s) 408, 425
MboI GATC 2 cut(s) 406, 423
MboII GAAGA 3 cut(s) 54, 409, 1398
MfeI CAATTG 1 cut(s) 1371
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 3 cut(s) 469, 535, 798
MlsI TGGCCA 2 cut(s) 221, 947
MluCI AATT 6 cut(s) 148, 895, 1095, 1345, 1371, 1427
MluNI TGGCCA 2 cut(s) 221, 947
MlyI GAGTC 2 cut(s) 580, 1360
MmeI TCCRAC 1 cut(s) 186
Mox20I TGGCCA 2 cut(s) 221, 947
Mph1103I ATGCAT 1 cut(s) 1035
MroXI GAANNNNTTC 1 cut(s) 308
MscI TGGCCA 2 cut(s) 221, 947
MseI TTAA 3 cut(s) 807, 1155, 1430
MslI CAYNNNNRTG 2 cut(s) 14, 1421
Msp20I TGGCCA 2 cut(s) 221, 947
MspA1I CMGCKG 1 cut(s) 1300
MspI CCGG 6 cut(s) 109, 200, 245, 320, 563, 700
MspR9I CCNGG 5 cut(s) 85, 201, 320, 700, 732
MunI CAATTG 1 cut(s) 1371
MvaI CCWGG 2 cut(s) 85, 732
MvnI CGCG 3 cut(s) 128, 197, 215
MwoI GCNNNNNNNGC 4 cut(s) 25, 241, 700, 852
NciI CCSGG 3 cut(s) 201, 320, 700
NcoI CCATGG 1 cut(s) 1121
NdeII GATC 2 cut(s) 406, 423
NheI GCTAGC 2 cut(s) 4, 928
NlaIII CATG 9 cut(s) 19, 294, 302, 437, 483, 915, 968, 1125, 1426
NlaIV GGNNCC 4 cut(s) 324, 534, 847, 1008
NmeAIII GCCGAG 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 1194
NsbI TGCGCA 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 1035
NspI RCATGY 2 cut(s) 968, 1426
PaeI GCATGC 1 cut(s) 1426
PauI GCGCGC 1 cut(s) 195
PciI ACATGT 1 cut(s) 964
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 7 cut(s) 209, 248, 400, 495, 908, 973, 1408
PflFI GACNNNGTC 1 cut(s) 382
PflMI CCANNNNNTGG 1 cut(s) 21
PfoI TCCNGGA 1 cut(s) 730
PleI GAGTC 2 cut(s) 580, 1360
PpsI GAGTC 2 cut(s) 580, 1360
Ppu21I YACGTR 1 cut(s) 374
PscI ACATGT 1 cut(s) 964
PsiI TTATAA 1 cut(s) 1286
Psp1406I AACGTT 1 cut(s) 363
Psp6I CCWGG 2 cut(s) 83, 730
PspFI CCCAGC 3 cut(s) 798, 1296, 1412
PspGI CCWGG 2 cut(s) 83, 730
PspN4I GGNNCC 4 cut(s) 324, 534, 847, 1008
PsrI GAACNNNNNNTAC 2 cut(s) 613, 645
PstI CTGCAG 2 cut(s) 1147, 1305
PsuI RGATCY 1 cut(s) 423
PsyI GACNNNGTC 1 cut(s) 382
PteI GCGCGC 1 cut(s) 195
PvuII CAGCTG 1 cut(s) 1300
RsaI GTAC 3 cut(s) 372, 759, 1090
RsaNI GTAC 3 cut(s) 371, 758, 1089
RseI CAYNNNNRTG 2 cut(s) 14, 1421
SaqAI TTAA 3 cut(s) 807, 1155, 1430
Sau3AI GATC 2 cut(s) 406, 423
SchI GAGTC 2 cut(s) 580, 1360
ScrFI CCNGG 5 cut(s) 85, 201, 320, 700, 732
SduI GDGCHC 3 cut(s) 469, 535, 798
SfaNI GCATC 4 cut(s) 689, 703, 1008, 1020
SfcI CTRYAG 2 cut(s) 1143, 1301
SmiMI CAYNNNNRTG 2 cut(s) 14, 1421
SpeI ACTAGT 1 cut(s) 473
SphI GCATGC 1 cut(s) 1426
Sse9I AATT 6 cut(s) 148, 895, 1095, 1345, 1371, 1427
SsiI CCGC 7 cut(s) 96, 126, 213, 227, 233, 259, 1445
SspMI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 929
StyD4I CCNGG 5 cut(s) 83, 199, 318, 698, 730
StyI CCWWGG 1 cut(s) 1121
TaaI ACNGT 3 cut(s) 170, 382, 644
TaiI ACGT 2 cut(s) 366, 376
TaqI TCGA 7 cut(s) 113, 134, 367, 409, 447, 667, 1279
TasI AATT 6 cut(s) 148, 895, 1095, 1345, 1371, 1427
TatI WGTACW 1 cut(s) 1088
TauI GCSGC 3 cut(s) 128, 235, 1448
TfiI GAWTC 7 cut(s) 209, 248, 400, 495, 908, 973, 1408
Tru1I TTAA 3 cut(s) 807, 1155, 1430
Tru9I TTAA 3 cut(s) 807, 1155, 1430
TscAI CASTG 3 cut(s) 334, 387, 862
TseFI GTSAC 1 cut(s) 1194
TseI GCWGC 7 cut(s) 229, 518, 1142, 1145, 1224, 1259, 1300
Tsp45I GTSAC 1 cut(s) 1194
TspDTI ATGAA 6 cut(s) 639, 900, 1149, 1178, 1358, 1389
TspGWI ACGGA 1 cut(s) 525
TspRI CASTG 3 cut(s) 334, 387, 862
Tth111I GACNNNGTC 1 cut(s) 382
Van91I CCANNNNNTGG 1 cut(s) 21
VneI GTGCAC 1 cut(s) 465
XapI RAATTY 1 cut(s) 1345
XceI RCATGY 2 cut(s) 968, 1426
XmnI GAANNNNTTC 1 cut(s) 308
XspI CTAG 9 cut(s) 5, 30, 390, 474, 492, 654, 704, 753, 929
Zsp2I ATGCAT 1 cut(s) 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.