Rh6BG412200

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001271
Physical Location & Seq
Reverse (-)
63750914 .. 63756139
5226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG412200.1

Sequence Viewer

Length: 1998 bp
ATGACTCATTTTGATATATTTGAAGGGGGCATTCTGACATTTTCAACCGACGGCTTCAACAACACCCACAGCAGCACCACCAGTTGCCACCGCCTACCACTTCCACAACCTTCTTCTTCAACGTTTCCCAATCACCTAATGCCTTCTTCTTCTTCTGCTCCATCTCAATCTCATCAAAAACGAACCTTTTTTACATCTTCATCTTCTACTCCTGCTTCCTCCTCCTCCTCTGTCTCAGCTCTGAGACCGGGGATCAACAGAAGCGGCAGTGATGTTAACAGCCCACGGCGGCTCACGCGCCAGAGGAAGCTGCGCTACCTCACGAGCCCCGAGGATCTCGACTCTTACGGGGTCCCGGAGAAGTCTCGCTCATCGCCCACCTCGCCTGAATATTCGACCCGTAGATCACGCTCTCCGTGTGGCGAGTCAAATCCAAATTCACGCTTTCCCGGTGGCGAGTCGGCCCCATGTTCTCGGTCTTCGGTCGGTGACTCGGCCAGAAAGTCATGCTCCTCTTCTTTCGTTGCTGGGCCTCAAGGCCCACAACCCTTACCGCTTCCGGAGAACAACCGGAGACCCGACTGCGTCTCTAAAGATAACAACTTCTTCAGCCCTGTGGTCGGCAGGAAGAGCGTCGATCAAGCTGCCACAAGCTCATCATCACCGGCGAAATCTTCCAAGCACCGAAGCCGAGATGTAAACAATTCCGGCGGGGGTGGGAGTGGGGGTTTTAATTTCCATTTGGGTTTGAATGTTCAATCTAGGACTGTTTTTAGCTCAAGTCATCATCATCCTGTTAGCCCACAAAGATCAAACACAGTGGATTTTTTGCCTTGTCAGTCACATGTTGCAGGCAACTTTTATAAACACAGGGGAATGGGAATGGGAATGGCAGTGTCACCGGAGGCTAACTTTGAACGTGAAACTTACAACTTGAGCAGGAGTGCTCCAACCAGTGTTCTCTCAAGTCCTGCAGTTAGCCCGCGCCGATCAACCACTGGGGATCCTTTTCCTTTTTTCATGACTCATCAAGATCAATATCAGGATGGTGCTACCTTTCTCTCGTTGTTGTCACCAAAGAAGCCTACACTCAGTCCCACTCATTCTCCACTCCATAGCCCTACGCCTCGAAGTCCACGCTTCAGCCCCAATGCTTCAAACTATGAGAGTAGTCTTGCCACTGCTCACCCGTTGCCTCTGCCTCCTGGAGCCGCTGTGCCAGCACCGCCGCCGTCTATCACTATGCATCAAAATAATGTAGCACCAAATGTAACCGTATCATCAATGAAAGGTCAATGGCAGAAGGGGAAACTTATCGGACGGGGTACCTTTGGTAGTGTTTATCTTGCAACCAACCGAGAAACTGGGGCCTTGTGTGCAATGAAGGAAGTTGATCTCATTCCTGATGATCCTAAATCTGCAGAAAGTATAAAGCAATTGGAGCAGGAAATTAAAGTCCTCCGTACACTAAAGCATCCAAATATTGTGCAGTACTATGGTAGTGAAGTGATTGATGATCATTTCTACATATATTTAGAATATGTTCACCCTGGATCAATAAATAAATATGTCCAAGACCATCTTGGAGCTATGACTGAATCTGTAATCCGCAATTTTACCCGCCATATCCTCCGTGGGTTGGCTTTTTTGCATGACACGAAGACAATTCACAGGGATATTAAAGGTGCAAATTTGCTTGTTGATGCATCAGGCGTTGTCAAACTTGCCGATTTTGGGATGGCAAAACATCTTAGTGCGCATTCATACAATCTTTCTTTGAAGGGCAGCCCATACTGGATGGCTCCTGAGGTCATAAAGGCTGTGATACAGAATGATGGTGATCCTAATCTTGCTTTGGCTGTTGATATATGGAGCTTGGGTTGTACCATCATCGAGATGTTCAATGGAAAACCGCCTTGGAGTGAATTTGAAGGGGTAAGTTTCAAATTTGTAGTACAACTTGCTGTTTTTGAGCAACTATCTTTGTGGTTTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

665

Amino Acids

72.47

Weight (kDa)

9.31

Isoelectric Point (pI)

65.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 433 - 642 5.9e-59 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 436 - 646 2.1e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
Acc16I TGCGCA 1 cut(s) 1758
Acc65I GGTACC 1 cut(s) 1325
AccB1I GGYRCC 1 cut(s) 1325
AccII CGCG 2 cut(s) 298, 985
AccIII TCCGGA 1 cut(s) 559
AclI AACGTT 1 cut(s) 122
AclWI GGATC 7 cut(s) 260, 342, 998, 1011, 1403, 1561, 1835
AcoI YGGCCR 1 cut(s) 495
AcsI RAATTY 4 cut(s) 436, 1690, 1925, 1946
AcuI CTGAAG 2 cut(s) 592, 1126
AfaI GTAC 5 cut(s) 1327, 1465, 1493, 1885, 1956
AfiI CCNNNNNNNGG 3 cut(s) 620, 1639, 1734
AflIII ACRYGT 1 cut(s) 844
AjnI CCWGG 2 cut(s) 1204, 1549
AjuI GAANNNNNNNTTGG 2 cut(s) 1900, 1932
AluBI AGCT 7 cut(s) 239, 310, 644, 654, 777, 1589, 1875
AluI AGCT 7 cut(s) 239, 310, 644, 654, 777, 1589, 1875
Alw21I GWGCWC 1 cut(s) 949
Alw26I GTCTC 5 cut(s) 238, 238, 369, 568, 592
AlwI GGATC 7 cut(s) 260, 342, 998, 1011, 1403, 1561, 1835
Ama87I CYCGRG 1 cut(s) 329
Aor13HI TCCGGA 1 cut(s) 559
AoxI GGCC 5 cut(s) 462, 495, 530, 538, 1368
ApeKI GCWGC 4 cut(s) 72, 310, 644, 1785
ApoI RAATTY 4 cut(s) 436, 1690, 1925, 1946
Asp700I GAANNNNTTC 1 cut(s) 1542
Asp718I GGTACC 1 cut(s) 1325
AspLEI GCGC 4 cut(s) 300, 315, 987, 1759
AspS9I GGNCC 5 cut(s) 352, 463, 530, 539, 1368
AsuC2I CCSGG 3 cut(s) 249, 356, 450
AsuHPI GGTGA 8 cut(s) 125, 500, 654, 891, 1065, 1178, 1538, 1850
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 1 cut(s) 352
AxyI CCTNAGG 1 cut(s) 1806
BamHI GGATCC 1 cut(s) 1003
BanI GGYRCC 1 cut(s) 1325
BanII GRGCYC 1 cut(s) 329
BarI GAAGNNNNNNTAC 2 cut(s) 299, 331
BauI CACGAG 1 cut(s) 322
BbsI GAAGAC 2 cut(s) 471, 1667
Bbv12I GWGCWC 1 cut(s) 949
BbvI GCAGC 4 cut(s) 84, 297, 631, 1797
BccI CCATC 7 cut(s) 169, 1040, 1587, 1732, 1792, 1829, 1895
BceAI ACGGC 3 cut(s) 67, 302, 1216
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 2 cut(s) 1206, 1551
BclI TGATCA 1 cut(s) 1516
BcnI CCSGG 3 cut(s) 249, 356, 450
BcoDI GTCTC 5 cut(s) 238, 238, 369, 568, 592
BfaI CTAG 1 cut(s) 762
BfmI CTRYAG 2 cut(s) 972, 1419
BisI GCNGC 8 cut(s) 73, 265, 290, 311, 645, 1212, 1229, 1786
BlsI GCNGC 8 cut(s) 74, 266, 291, 312, 646, 1213, 1230, 1787
BmcAI AGTACT 1 cut(s) 1493
Bme1390I CCNGG 5 cut(s) 249, 356, 450, 1206, 1551
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 5 cut(s) 352, 463, 530, 539, 1368
BmiI GGNNCC 8 cut(s) 353, 354, 465, 1005, 1210, 1327, 1369, 1803
BmrFI CCNGG 5 cut(s) 249, 356, 450, 1206, 1551
BmrI ACTGGG 2 cut(s) 1008, 1374
BmsI GCATC 4 cut(s) 1255, 1483, 1693, 1715
BmuI ACTGGG 2 cut(s) 1008, 1374
BpiI GAAGAC 2 cut(s) 471, 1667
BpmI CTGGAG 1 cut(s) 1227
BpuEI CTTGAG 4 cut(s) 519, 763, 949, 955
BpuMI CCSGG 3 cut(s) 249, 356, 450
BsaBI GATNNNNATC 3 cut(s) 1038, 1839, 1845
BsaI GGTCTC 2 cut(s) 238, 568
BsaJI CCNNGG 6 cut(s) 248, 284, 330, 1549, 1633, 1916
BsaWI WCCGGW 3 cut(s) 559, 570, 901
BsaXI ACNNNNNCTCC 8 cut(s) 712, 742, 1090, 1120, 1200, 1230, 1864, 1894
Bsc4I CCNNNNNNNGG 3 cut(s) 620, 1639, 1734
Bse118I RCCGGY 1 cut(s) 664
Bse1I ACTGG 5 cut(s) 81, 954, 1003, 1369, 1799
Bse21I CCTNAGG 1 cut(s) 1806
Bse3DI GCAATG 1 cut(s) 1386
Bse8I GATNNNNATC 3 cut(s) 1038, 1839, 1845
BseAI TCCGGA 1 cut(s) 559
BseBI CCWGG 2 cut(s) 1206, 1551
BseDI CCNNGG 6 cut(s) 248, 284, 330, 1549, 1633, 1916
BseGI GGATG 5 cut(s) 790, 1051, 1474, 1743, 1803
BseJI GATNNNNATC 3 cut(s) 1038, 1839, 1845
BseLI CCNNNNNNNGG 3 cut(s) 620, 1639, 1734
BseMI GCAATG 1 cut(s) 1386
BseMII CTCAG 4 cut(s) 233, 249, 1105, 1797
BseNI ACTGG 5 cut(s) 81, 954, 1003, 1369, 1799
BseRI GAGGAG 4 cut(s) 211, 214, 217, 502
BseXI GCAGC 4 cut(s) 84, 297, 631, 1797
BseYI CCCAGC 1 cut(s) 527
BsgI GTGCAG 1 cut(s) 1508
Bsh1236I CGCG 2 cut(s) 298, 985
Bsh1285I CGRYCG 1 cut(s) 486
BshFI GGCC 5 cut(s) 464, 497, 532, 540, 1370
BshNI GGYRCC 1 cut(s) 1325
BsiEI CGRYCG 1 cut(s) 486
BsiHKAI GWGCWC 1 cut(s) 949
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 8 cut(s) 248, 356, 450, 560, 571, 665, 708, 902
BslFI GGGAC 2 cut(s) 338, 1080
BslI CCNNNNNNNGG 3 cut(s) 620, 1639, 1734
BsmAI GTCTC 5 cut(s) 238, 238, 369, 568, 592
BsmBI CGTCTC 1 cut(s) 592
BsmFI GGGAC 2 cut(s) 338, 1080
BsmI GAATGC 2 cut(s) 30, 1759
BsnI GGCC 5 cut(s) 464, 497, 532, 540, 1370
Bso31I GGTCTC 2 cut(s) 238, 568
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 2 cut(s) 329, 949
Bsp13I TCCGGA 1 cut(s) 559
BspANI GGCC 5 cut(s) 464, 497, 532, 540, 1370
BspCNI CTCAG 4 cut(s) 234, 248, 1104, 1798
BspEI TCCGGA 1 cut(s) 559
BspFNI CGCG 2 cut(s) 298, 985
BspHI TCATGA 1 cut(s) 1020
BspLI GGNNCC 8 cut(s) 353, 354, 465, 1005, 1210, 1327, 1369, 1803
BspMAI CTGCAG 2 cut(s) 976, 1423
BspPI GGATC 7 cut(s) 260, 342, 998, 1011, 1403, 1561, 1835
BspQI GCTCTTC 1 cut(s) 623
BspT107I GGYRCC 1 cut(s) 1325
BspTNI GGTCTC 2 cut(s) 238, 568
BsrDI GCAATG 1 cut(s) 1386
BsrFI RCCGGY 1 cut(s) 664
BsrI ACTGG 5 cut(s) 81, 954, 1003, 1369, 1799
BssAI RCCGGY 1 cut(s) 664
BssECI CCNNGG 6 cut(s) 248, 284, 330, 1549, 1633, 1916
BssSI CACGAG 1 cut(s) 322
BssT1I CCWWGG 1 cut(s) 1916
Bst2BI CACGAG 1 cut(s) 322
Bst2UI CCWGG 2 cut(s) 1206, 1551
Bst4CI ACNGT 3 cut(s) 769, 820, 1276
Bst6I CTCTTC 2 cut(s) 520, 623
BstC8I GCNNGC 3 cut(s) 853, 983, 1221
BstDEI CTNAG 5 cut(s) 235, 242, 1091, 1751, 1806
BstDSI CCRYGG 2 cut(s) 284, 1633
BstF5I GGATG 5 cut(s) 790, 1051, 1474, 1743, 1803
BstFNI CGCG 2 cut(s) 298, 985
BstHHI GCGC 4 cut(s) 300, 315, 987, 1759
BstMAI GTCTC 5 cut(s) 238, 238, 369, 568, 592
BstMCI CGRYCG 1 cut(s) 486
BstMWI GCNNNNNNNGC 7 cut(s) 295, 382, 630, 1220, 1225, 1376, 1441
BstNI CCWGG 2 cut(s) 1206, 1551
BstNSI RCATGY 1 cut(s) 848
BstSCI CCNGG 5 cut(s) 247, 354, 448, 1204, 1549
BstSFI CTRYAG 2 cut(s) 972, 1419
BstUI CGCG 2 cut(s) 298, 985
BstV1I GCAGC 4 cut(s) 84, 297, 631, 1797
BstV2I GAAGAC 2 cut(s) 471, 1667
BstX2I RGATCY 2 cut(s) 334, 1003
BstYI RGATCY 2 cut(s) 334, 1003
Bsu36I CCTNAGG 1 cut(s) 1806
BsuRI GGCC 5 cut(s) 464, 497, 532, 540, 1370
BtgI CCRYGG 2 cut(s) 284, 1633
BtgZI GCGATG 1 cut(s) 357
BtsCI GGATG 5 cut(s) 790, 1051, 1474, 1743, 1803
BtsI GCAGTG 3 cut(s) 274, 900, 1179
BtsIMutI CAGTG 6 cut(s) 274, 825, 900, 961, 996, 1179
Cac8I GCNNGC 3 cut(s) 853, 983, 1221
CciI TCATGA 1 cut(s) 1020
CfoI GCGC 4 cut(s) 300, 315, 987, 1759
Cfr10I RCCGGY 1 cut(s) 664
Cfr13I GGNCC 5 cut(s) 352, 463, 530, 539, 1368
CseI GACGC 2 cut(s) 574, 622
Csp6I GTAC 5 cut(s) 1326, 1464, 1492, 1884, 1955
CspCI CAANNNNNGTGG 2 cut(s) 801, 836
CviAII CATG 5 cut(s) 468, 507, 845, 1021, 1652
CviQI GTAC 5 cut(s) 1326, 1464, 1492, 1884, 1955
DdeI CTNAG 5 cut(s) 235, 242, 1091, 1751, 1806
EaeI YGGCCR 1 cut(s) 495
Eam1104I CTCTTC 2 cut(s) 520, 623
EarI CTCTTC 2 cut(s) 520, 623
Eco130I CCWWGG 1 cut(s) 1916
Eco24I GRGCYC 1 cut(s) 329
Eco31I GGTCTC 2 cut(s) 238, 568
Eco47I GGWCC 1 cut(s) 352
Eco57I CTGAAG 2 cut(s) 592, 1126
Eco81I CCTNAGG 1 cut(s) 1806
Eco88I CYCGRG 1 cut(s) 329
EcoO109I RGGNCCY 2 cut(s) 352, 1368
EcoRII CCWGG 2 cut(s) 1204, 1549
EcoT14I CCWWGG 1 cut(s) 1916
EcoT22I ATGCAT 2 cut(s) 1248, 1708
EcoT38I GRGCYC 1 cut(s) 329
ErhI CCWWGG 1 cut(s) 1916
Esp3I CGTCTC 1 cut(s) 592
FaeI CATG 5 cut(s) 471, 510, 848, 1024, 1655
FalI AAGNNNNNCTT 2 cut(s) 1566, 1598
FaqI GGGAC 2 cut(s) 338, 1080
FatI CATG 5 cut(s) 467, 506, 844, 1020, 1651
FauI CCCGC 3 cut(s) 704, 990, 1628
FbaI TGATCA 1 cut(s) 1516
Fnu4HI GCNGC 8 cut(s) 73, 265, 290, 311, 645, 1212, 1229, 1786
FokI GGATG 5 cut(s) 777, 1058, 1461, 1750, 1810
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 8 cut(s) 73, 265, 290, 311, 645, 1212, 1229, 1786
FspAI RTGCGCAY 1 cut(s) 1758
FspBI CTAG 1 cut(s) 762
FspI TGCGCA 1 cut(s) 1758
GlaI GCGC 4 cut(s) 299, 314, 986, 1758
GluI GCNGC 8 cut(s) 73, 265, 290, 311, 645, 1212, 1229, 1786
GsaI CCCAGC 1 cut(s) 531
GsuI CTGGAG 1 cut(s) 1227
HaeIII GGCC 5 cut(s) 464, 497, 532, 540, 1370
HapII CCGG 8 cut(s) 248, 356, 450, 560, 571, 665, 708, 902
HgaI GACGC 2 cut(s) 574, 622
HhaI GCGC 4 cut(s) 300, 315, 987, 1759
Hin1II CATG 5 cut(s) 471, 510, 848, 1024, 1655
Hin6I GCGC 4 cut(s) 298, 313, 985, 1757
HinP1I GCGC 4 cut(s) 298, 313, 985, 1757
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 7 cut(s) 4, 341, 425, 458, 491, 1024, 1598
HpaI GTTAAC 1 cut(s) 277
HpaII CCGG 8 cut(s) 248, 356, 450, 560, 571, 665, 708, 902
HphI GGTGA 8 cut(s) 125, 500, 654, 891, 1065, 1178, 1538, 1850
Hpy166II GTNNAC 5 cut(s) 277, 700, 1136, 1466, 1546
Hpy188I TCNGA 3 cut(s) 36, 243, 1319
Hpy188III TCNNGA 9 cut(s) 322, 338, 560, 1021, 1031, 1043, 1403, 1805, 1894
Hpy8I GTNNAC 5 cut(s) 277, 700, 1136, 1466, 1546
Hpy99I CGWCG 2 cut(s) 53, 638
HpyAV CCTTC 7 cut(s) 17, 120, 153, 1297, 1378, 1774, 1925
HpyCH4III ACNGT 3 cut(s) 769, 820, 1276
HpyCH4IV ACGT 2 cut(s) 122, 919
HpyF10VI GCNNNNNNNGC 7 cut(s) 295, 382, 630, 1220, 1225, 1376, 1441
HpyF3I CTNAG 5 cut(s) 235, 242, 1091, 1751, 1806
HpySE526I ACGT 2 cut(s) 122, 919
Hsp92II CATG 5 cut(s) 471, 510, 848, 1024, 1655
HspAI GCGC 4 cut(s) 298, 313, 985, 1757
KflI GGGWCCC 1 cut(s) 352
Kpn2I TCCGGA 1 cut(s) 559
KpnI GGTACC 1 cut(s) 1329
Ksp22I TGATCA 1 cut(s) 1516
KspAI GTTAAC 1 cut(s) 277
LguI GCTCTTC 1 cut(s) 623
LmnI GCTCC 8 cut(s) 163, 515, 952, 1208, 1441, 1586, 1807, 1872
Lsp1109I GCAGC 4 cut(s) 84, 297, 631, 1797
LweI GCATC 4 cut(s) 1255, 1483, 1693, 1715
MaeI CTAG 1 cut(s) 762
MaeII ACGT 2 cut(s) 122, 919
MaeIII GTNAC 5 cut(s) 488, 840, 897, 1071, 1270
MfeI CAATTG 1 cut(s) 1436
MflI RGATCY 2 cut(s) 334, 1003
MhlI GDGCHC 2 cut(s) 329, 949
MlyI GAGTC 5 cut(s) 335, 434, 467, 485, 1018
MmeI TCCRAC 1 cut(s) 974
Mph1103I ATGCAT 2 cut(s) 1248, 1708
MroI TCCGGA 1 cut(s) 559
MroXI GAANNNNTTC 1 cut(s) 1542
MseI TTAA 5 cut(s) 276, 732, 1452, 1680, 1996
MslI CAYNNNNRTG 2 cut(s) 1752, 1895
MspA1I CMGCKG 1 cut(s) 1214
MspI CCGG 8 cut(s) 248, 356, 450, 560, 571, 665, 708, 902
MspR9I CCNGG 5 cut(s) 249, 356, 450, 1206, 1551
MunI CAATTG 1 cut(s) 1436
Mva1269I GAATGC 2 cut(s) 30, 1759
MvaI CCWGG 2 cut(s) 1206, 1551
MvnI CGCG 2 cut(s) 298, 985
MwoI GCNNNNNNNGC 7 cut(s) 295, 382, 630, 1220, 1225, 1376, 1441
NciI CCSGG 3 cut(s) 249, 356, 450
NlaIII CATG 5 cut(s) 471, 510, 848, 1024, 1655
NlaIV GGNNCC 8 cut(s) 353, 354, 465, 1005, 1210, 1327, 1369, 1803
NmeAIII GCCGAG 2 cut(s) 473, 716
NmuCI GTSAC 4 cut(s) 488, 840, 897, 1071
NsbI TGCGCA 1 cut(s) 1758
NsiI ATGCAT 2 cut(s) 1248, 1708
NspI RCATGY 1 cut(s) 848
PagI TCATGA 1 cut(s) 1020
PciI ACATGT 1 cut(s) 844
PciSI GCTCTTC 1 cut(s) 623
PctI GAATGC 2 cut(s) 30, 1759
PdmI GAANNNNTTC 1 cut(s) 1542
PfeI GAWTC 1 cut(s) 1598
PflFI GACNNNGTC 1 cut(s) 584
PfoI TCCNGGA 2 cut(s) 354, 1204
PkrI GCNGC 8 cut(s) 74, 266, 291, 312, 646, 1213, 1230, 1787
PleI GAGTC 5 cut(s) 335, 433, 466, 485, 1018
PpsI GAGTC 5 cut(s) 335, 433, 466, 485, 1018
PpuMI RGGWCCY 1 cut(s) 352
PscI ACATGT 1 cut(s) 844
PsiI TTATAA 1 cut(s) 864
Psp1406I AACGTT 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 352
Psp6I CCWGG 2 cut(s) 1204, 1549
PspFI CCCAGC 1 cut(s) 527
PspGI CCWGG 2 cut(s) 1204, 1549
PspN4I GGNNCC 8 cut(s) 353, 354, 465, 1005, 1210, 1327, 1369, 1803
PspPI GGNCC 5 cut(s) 352, 463, 530, 539, 1368
PspPPI RGGWCCY 1 cut(s) 352
PsrI GAACNNNNNNTAC 2 cut(s) 175, 207
PstI CTGCAG 2 cut(s) 976, 1423
PsuI RGATCY 2 cut(s) 334, 1003
PsyI GACNNNGTC 1 cut(s) 584
RsaI GTAC 5 cut(s) 1327, 1465, 1493, 1885, 1956
RsaNI GTAC 5 cut(s) 1326, 1464, 1492, 1884, 1955
RseI CAYNNNNRTG 2 cut(s) 1752, 1895
SapI GCTCTTC 1 cut(s) 623
SaqAI TTAA 5 cut(s) 276, 732, 1452, 1680, 1996
SatI GCNGC 8 cut(s) 73, 265, 290, 311, 645, 1212, 1229, 1786
Sau96I GGNCC 5 cut(s) 352, 463, 530, 539, 1368
ScaI AGTACT 1 cut(s) 1493
SchI GAGTC 5 cut(s) 335, 434, 467, 485, 1018
ScrFI CCNGG 5 cut(s) 249, 356, 450, 1206, 1551
SduI GDGCHC 2 cut(s) 329, 949
SfaNI GCATC 4 cut(s) 1255, 1483, 1693, 1715
SfcI CTRYAG 2 cut(s) 972, 1419
SgrAI CRCCGGYG 1 cut(s) 664
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 2 cut(s) 1752, 1895
SmlI CTYRAG 4 cut(s) 534, 778, 934, 964
SmoI CTYRAG 4 cut(s) 534, 778, 934, 964
SspI AATATT 2 cut(s) 392, 1483
SspMI CTAG 1 cut(s) 762
StyD4I CCNGG 5 cut(s) 247, 354, 448, 1204, 1549
StyI CCWWGG 1 cut(s) 1916
TaaI ACNGT 3 cut(s) 769, 820, 1276
TaiI ACGT 2 cut(s) 125, 922
TaqI TCGA 5 cut(s) 339, 395, 636, 1129, 1893
TaqII GACCGA 2 cut(s) 465, 472
TatI WGTACW 2 cut(s) 1491, 1954
TauI GCSGC 4 cut(s) 267, 292, 1214, 1231
TfiI GAWTC 1 cut(s) 1598
Tru1I TTAA 5 cut(s) 276, 732, 1452, 1680, 1996
Tru9I TTAA 5 cut(s) 276, 732, 1452, 1680, 1996
TscAI CASTG 6 cut(s) 274, 825, 900, 961, 1003, 1186
TseFI GTSAC 4 cut(s) 488, 840, 897, 1071
TseI GCWGC 4 cut(s) 72, 310, 644, 1785
Tsp45I GTSAC 4 cut(s) 488, 840, 897, 1071
TspDTI ATGAA 5 cut(s) 189, 1009, 1301, 1397, 1752
TspGWI ACGGA 3 cut(s) 405, 1451, 1622
TspRI CASTG 6 cut(s) 274, 825, 900, 961, 1003, 1186
Tth111I GACNNNGTC 1 cut(s) 584
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 4 cut(s) 436, 1690, 1925, 1946
XceI RCATGY 1 cut(s) 848
XcmI CCANNNNNNNNNTGG 2 cut(s) 1580, 1631
XmnI GAANNNNTTC 1 cut(s) 1542
XspI CTAG 1 cut(s) 762
ZrmI AGTACT 1 cut(s) 1493
Zsp2I ATGCAT 2 cut(s) 1248, 1708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.