Rmu_sc0003419.1_g000027

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003419.1
OG0001271
Physical Location & Seq
Reverse (-)
89776 .. 94370
4595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003419.1_g000027.1.cds

Sequence Viewer

Length: 1395 bp
atgaattatgctatgagtttggtgttgaaaatgtgtttcagtgtgttttcttggcgaggcactccaaagaataacgttcaagtacgtgagacagtgtcgtctaggaggaagaatcgagatcgaaatcgtttacagatccctgaacatgacttcaggctcgatgttcctgttagaagtgcacccactagtccatgcgcaagtcctagattcagcccacgccaagtgctgctgatttgtgggctccgtccagaaatcaagtctggggtagaagtccccttcgacccctacaaggatcatccccattgcatccccgagtcccagccgattagagggcaatggcaaaagggaaagcttattggtcgtggcacgtttggcagtgtttatgttgctacaaatagagagactggagctttatgtgcaatgaaggaagttgaactacttcctgatgacccgaaatcagcagagtgtataaagcagctacagcaggaaatcaaaggcctcagccagctcaagcatcctaatattgtgcagtactatggaagtgaaattctgatgcaatctgagatgcataaagataatagctctgatttggctcttgctgttgatatatggagtttgggttgtcctattattgaaatgttcacaggaaaaccaccatggagtgagtatgaaggggctgcagctatgtttaaagttatgaaggatactccaccatttccagaaacattgtcgcctgagggtaaggatttccttaggtgctgcttccaaagaaatcctgctgagaggtcaactgctgcaatgctattagaacatcgatttataaaaaactcccagctgcaggaagtcccatctacgacctataatgcctctaatgggatgaatttcttgggaacaaggaataagatgaaggacacgttggtttcaacaatagacatacagcaggaggtcgagggaattgaagggagtggtgttaaagaaaatcaacctgacattaaaggttcatgcgaagtacatgaacctccatcccgattcttcaagaggccgttctcagttccttcaccagaaaagcatacgagggcgcttctagcaagggtgctggggttttttaagtggaaggagcagggtagcatgacaaggcttcagaagagtttcgggtatgtgactggagaacaggttaaggagcttggagaccaggttgagggtggagcggggctagataagagctttgggttctcaaagaatttgattaaaatgtatgagcttggaagtatggttgggttcgggcatttttcctacacttgcatagctacttttaagaagggaaagcttgagggccaccatgctgctgtcaaggtcatctccaaagccaaggtttgctaccttcatttgttctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

464

Amino Acids

52.18

Weight (kDa)

9.04

Isoelectric Point (pI)

43.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 821
AasI GACNNNNNNGTC 1 cut(s) 97
Acc16I TGCGCA 1 cut(s) 196
AccBSI CCGCTC 1 cut(s) 1208
AciI CCGC 1 cut(s) 1208
AclI AACGTT 1 cut(s) 75
AclWI GGATC 2 cut(s) 130, 300
AcsI RAATTY 3 cut(s) 546, 880, 1240
AcuI CTGAAG 2 cut(s) 136, 1124
AfaI GTAC 3 cut(s) 84, 533, 1011
AfiI CCNNNNNNNGG 5 cut(s) 289, 329, 838, 873, 1198
AflIII ACRYGT 1 cut(s) 912
AgsI TTSAA 7 cut(s) 28, 80, 434, 635, 924, 959, 1036
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 1 cut(s) 1191
AjuI GAANNNNNNNTTGG 2 cut(s) 899, 931
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 3 cut(s) 83, 395, 1182
Alw44I GTGCAC 1 cut(s) 177
AlwI GGATC 2 cut(s) 130, 300
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 3 cut(s) 496, 1040, 1333
ApaLI GTGCAC 1 cut(s) 177
ApeKI GCWGC 8 cut(s) 226, 475, 677, 680, 759, 794, 835, 1343
ApoI RAATTY 3 cut(s) 546, 880, 1240
Asp700I GAANNNNTTC 2 cut(s) 438, 1148
AspLEI GCGC 2 cut(s) 197, 1081
AspS9I GGNCC 1 cut(s) 1333
AsuHPI GGTGA 1 cut(s) 1050
AvaI CYCGRG 1 cut(s) 311
AxyI CCTNAGG 2 cut(s) 735, 752
BaeGI GKGCMC 1 cut(s) 181
BanII GRGCYC 1 cut(s) 243
BarI GAAGNNNNNNTAC 2 cut(s) 420, 452
Bbv12I GWGCWC 1 cut(s) 181
BbvCI CCTCAGC 1 cut(s) 500
BbvI GCAGC 8 cut(s) 213, 487, 664, 692, 746, 781, 822, 1330
BccI CCATC 2 cut(s) 856, 1030
BceAI ACGGC 1 cut(s) 1027
BciT130I CCWGG 1 cut(s) 1193
BciVI GTATCC 1 cut(s) 697
BcoDI GTCTC 3 cut(s) 83, 395, 1182
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 6 cut(s) 102, 186, 204, 1085, 1214, 1393
BfmI CTRYAG 3 cut(s) 479, 678, 836
BfoI RGCGCY 1 cut(s) 1082
BfuI GTATCC 1 cut(s) 697
BisI GCNGC 8 cut(s) 227, 476, 678, 681, 760, 795, 836, 1344
BlsI GCNGC 8 cut(s) 228, 477, 679, 682, 761, 796, 837, 1345
BmcAI AGTACT 1 cut(s) 533
Bme1390I CCNGG 1 cut(s) 1193
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 1 cut(s) 1333
BmiI GGNNCC 1 cut(s) 242
BmrFI CCNGG 1 cut(s) 1193
BmsI GCATC 4 cut(s) 315, 523, 543, 555
BpmI CTGGAG 2 cut(s) 426, 1185
Bpu10I CCTNAGC 1 cut(s) 500
BpuEI CTTGAG 2 cut(s) 494, 1349
Bsa29I ATCGAT 1 cut(s) 814
BsaAI YACGTR 1 cut(s) 86
BsaBI GATNNNNATC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 1182
BsaJI CCNNGG 2 cut(s) 656, 1368
BsaXI ACNNNNNCTCC 6 cut(s) 604, 634, 1158, 1179, 1188, 1209
Bsc4I CCNNNNNNNGG 5 cut(s) 289, 329, 838, 873, 1198
Bse1I ACTGG 2 cut(s) 409, 1168
Bse21I CCTNAGG 2 cut(s) 735, 752
Bse3DI GCAATG 4 cut(s) 301, 341, 426, 804
Bse8I GATNNNNATC 1 cut(s) 123
BseBI CCWGG 1 cut(s) 1193
BseCI ATCGAT 1 cut(s) 814
BseDI CCNNGG 2 cut(s) 656, 1368
BseGI GGATG 5 cut(s) 295, 306, 514, 882, 1022
BseJI GATNNNNATC 1 cut(s) 123
BseLI CCNNNNNNNGG 5 cut(s) 289, 329, 838, 873, 1198
BseMI GCAATG 4 cut(s) 301, 341, 426, 804
BseMII CTCAG 5 cut(s) 514, 552, 726, 771, 1062
BseNI ACTGG 2 cut(s) 409, 1168
BseSI GKGCMC 1 cut(s) 181
BseXI GCAGC 8 cut(s) 213, 487, 664, 692, 746, 781, 822, 1330
BseYI CCCAGC 3 cut(s) 318, 831, 1096
BsgI GTGCAG 1 cut(s) 548
BshFI GGCC 3 cut(s) 498, 1042, 1335
BshVI ATCGAT 1 cut(s) 814
BsiHKAI GWGCWC 1 cut(s) 181
BsiHKCI CYCGRG 1 cut(s) 311
BslFI GGGAC 3 cut(s) 257, 301, 830
BslI CCNNNNNNNGG 5 cut(s) 289, 329, 838, 873, 1198
BsmAI GTCTC 3 cut(s) 83, 395, 1182
BsmFI GGGAC 3 cut(s) 257, 301, 830
BsnI GGCC 3 cut(s) 498, 1042, 1335
Bso31I GGTCTC 1 cut(s) 1182
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 2 cut(s) 181, 243
Bsp143I GATC 3 cut(s) 118, 135, 292
Bsp19I CCATGG 1 cut(s) 656
BspACI CCGC 1 cut(s) 1208
BspANI GGCC 3 cut(s) 498, 1042, 1335
BspCNI CTCAG 5 cut(s) 513, 553, 727, 772, 1061
BspDI ATCGAT 1 cut(s) 814
BspLI GGNNCC 1 cut(s) 242
BspMAI CTGCAG 2 cut(s) 682, 840
BspPI GGATC 2 cut(s) 130, 300
BspTNI GGTCTC 1 cut(s) 1182
BsrBI CCGCTC 1 cut(s) 1208
BsrDI GCAATG 4 cut(s) 301, 341, 426, 804
BsrI ACTGG 2 cut(s) 409, 1168
BssECI CCNNGG 2 cut(s) 656, 1368
BssMI GATC 3 cut(s) 118, 135, 292
BssT1I CCWWGG 2 cut(s) 656, 1368
Bst2UI CCWGG 1 cut(s) 1193
Bst4CI ACNGT 1 cut(s) 94
Bst6I CTCTTC 1 cut(s) 1139
BstBAI YACGTR 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 506
BstDEI CTNAG 6 cut(s) 500, 561, 735, 752, 780, 1048
BstDSI CCRYGG 1 cut(s) 656
BstF5I GGATG 5 cut(s) 295, 306, 514, 882, 1022
BstH2I RGCGCY 1 cut(s) 1082
BstHHI GCGC 2 cut(s) 197, 1081
BstKTI GATC 3 cut(s) 121, 138, 295
BstMAI GTCTC 3 cut(s) 83, 395, 1182
BstMBI GATC 3 cut(s) 118, 135, 292
BstMWI GCNNNNNNNGC 4 cut(s) 372, 416, 481, 1085
BstNI CCWGG 1 cut(s) 1193
BstSCI CCNGG 1 cut(s) 1191
BstSFI CTRYAG 3 cut(s) 479, 678, 836
BstSLI GKGCMC 1 cut(s) 181
BstV1I GCAGC 8 cut(s) 213, 487, 664, 692, 746, 781, 822, 1330
BstX2I RGATCY 1 cut(s) 135
BstYI RGATCY 1 cut(s) 135
Bsu15I ATCGAT 1 cut(s) 814
Bsu36I CCTNAGG 2 cut(s) 735, 752
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 3 cut(s) 498, 1042, 1335
BsuTUI ATCGAT 1 cut(s) 814
BtgI CCRYGG 1 cut(s) 656
BtsCI GGATG 5 cut(s) 295, 306, 514, 882, 1022
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 3 cut(s) 46, 99, 382
Cac8I GCNNGC 1 cut(s) 506
CfoI GCGC 2 cut(s) 197, 1081
Cfr13I GGNCC 1 cut(s) 1333
ClaI ATCGAT 1 cut(s) 814
CsiI ACCWGGT 1 cut(s) 1191
Csp6I GTAC 3 cut(s) 83, 532, 1010
CviAII CATG 7 cut(s) 146, 192, 657, 1002, 1013, 1129, 1340
CviQI GTAC 3 cut(s) 83, 532, 1010
DdeI CTNAG 6 cut(s) 500, 561, 735, 752, 780, 1048
DpnI GATC 3 cut(s) 120, 137, 294
DpnII GATC 3 cut(s) 118, 135, 292
DraI TTTAAA 1 cut(s) 691
DrdI GACNNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 97
Eam1104I CTCTTC 1 cut(s) 1139
EarI CTCTTC 1 cut(s) 1139
Eco130I CCWWGG 2 cut(s) 656, 1368
Eco147I AGGCCT 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 1182
Eco57I CTGAAG 2 cut(s) 136, 1124
Eco81I CCTNAGG 2 cut(s) 735, 752
Eco88I CYCGRG 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 1191
EcoT14I CCWWGG 2 cut(s) 656, 1368
EcoT22I ATGCAT 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 243
ErhI CCWWGG 2 cut(s) 656, 1368
FaeI CATG 7 cut(s) 149, 195, 660, 1005, 1016, 1132, 1343
FaqI GGGAC 3 cut(s) 257, 301, 830
FatI CATG 7 cut(s) 145, 191, 656, 1001, 1012, 1128, 1339
FauI CCCGC 1 cut(s) 1201
Fnu4HI GCNGC 8 cut(s) 227, 476, 678, 681, 760, 795, 836, 1344
FokI GGATG 5 cut(s) 282, 293, 501, 889, 1009
FriOI GRGCYC 1 cut(s) 243
Fsp4HI GCNGC 8 cut(s) 227, 476, 678, 681, 760, 795, 836, 1344
FspBI CTAG 6 cut(s) 102, 186, 204, 1085, 1214, 1393
FspI TGCGCA 1 cut(s) 196
GlaI GCGC 2 cut(s) 196, 1080
GluI GCNGC 8 cut(s) 227, 476, 678, 681, 760, 795, 836, 1344
GsaI CCCAGC 3 cut(s) 322, 835, 1100
GsuI CTGGAG 2 cut(s) 426, 1185
HaeII RGCGCY 1 cut(s) 1082
HaeIII GGCC 3 cut(s) 498, 1042, 1335
HhaI GCGC 2 cut(s) 197, 1081
Hin1II CATG 7 cut(s) 149, 195, 660, 1005, 1016, 1132, 1343
Hin6I GCGC 2 cut(s) 195, 1079
HinP1I GCGC 2 cut(s) 195, 1079
HincII GTYRAC 1 cut(s) 789
HindII GTYRAC 1 cut(s) 789
HindIII AAGCTT 2 cut(s) 350, 1325
HinfI GANTC 4 cut(s) 112, 207, 314, 1029
HphI GGTGA 1 cut(s) 1050
Hpy166II GTNNAC 4 cut(s) 131, 179, 642, 789
Hpy188I TCNGA 4 cut(s) 552, 562, 586, 1143
Hpy188III TCNNGA 6 cut(s) 116, 248, 443, 719, 1026, 1036
Hpy8I GTNNAC 4 cut(s) 131, 179, 642, 789
HpyCH4III ACNGT 1 cut(s) 94
HpyCH4IV ACGT 4 cut(s) 75, 85, 368, 914
HpyF10VI GCNNNNNNNGC 4 cut(s) 372, 416, 481, 1085
HpyF3I CTNAG 6 cut(s) 500, 561, 735, 752, 780, 1048
HpySE526I ACGT 4 cut(s) 75, 85, 368, 914
Hsp92II CATG 7 cut(s) 149, 195, 660, 1005, 1016, 1132, 1343
HspAI GCGC 2 cut(s) 195, 1079
Kzo9I GATC 3 cut(s) 118, 135, 292
LmnI GCTCC 5 cut(s) 246, 407, 1117, 1180, 1205
Lsp1109I GCAGC 8 cut(s) 213, 487, 664, 692, 746, 781, 822, 1330
LweI GCATC 4 cut(s) 315, 523, 543, 555
MabI ACCWGGT 1 cut(s) 1191
MaeI CTAG 6 cut(s) 102, 186, 204, 1085, 1214, 1393
MaeII ACGT 4 cut(s) 75, 85, 368, 914
MaeIII GTNAC 1 cut(s) 1159
MalI GATC 3 cut(s) 120, 137, 294
MbiI CCGCTC 1 cut(s) 1208
MboI GATC 3 cut(s) 118, 135, 292
MboII GAAGA 3 cut(s) 121, 1024, 1156
MflI RGATCY 1 cut(s) 135
MhlI GDGCHC 2 cut(s) 181, 243
MluCI AATT 5 cut(s) 4, 546, 880, 954, 1240
MlyI GAGTC 1 cut(s) 323
Mph1103I ATGCAT 1 cut(s) 570
MroXI GAANNNNTTC 2 cut(s) 438, 1148
MseI TTAA 7 cut(s) 690, 972, 993, 1107, 1176, 1248, 1314
MspA1I CMGCKG 1 cut(s) 835
MspR9I CCNGG 1 cut(s) 1193
MvaI CCWGG 1 cut(s) 1193
MwoI GCNNNNNNNGC 4 cut(s) 372, 416, 481, 1085
NcoI CCATGG 1 cut(s) 656
NdeII GATC 3 cut(s) 118, 135, 292
NlaIII CATG 7 cut(s) 149, 195, 660, 1005, 1016, 1132, 1343
NlaIV GGNNCC 1 cut(s) 242
NmuCI GTSAC 1 cut(s) 1159
NsbI TGCGCA 1 cut(s) 196
NsiI ATGCAT 1 cut(s) 570
PceI AGGCCT 1 cut(s) 498
PdmI GAANNNNTTC 2 cut(s) 438, 1148
PfeI GAWTC 3 cut(s) 112, 207, 1029
PflFI GACNNNGTC 1 cut(s) 94
PkrI GCNGC 8 cut(s) 228, 477, 679, 682, 761, 796, 837, 1345
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 86
PsiI TTATAA 1 cut(s) 821
Psp1406I AACGTT 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 1191
PspFI CCCAGC 3 cut(s) 318, 831, 1096
PspGI CCWGG 1 cut(s) 1191
PspN4I GGNNCC 1 cut(s) 242
PspPI GGNCC 1 cut(s) 1333
PstI CTGCAG 2 cut(s) 682, 840
PsuI RGATCY 1 cut(s) 135
PsyI GACNNNGTC 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 835
RsaI GTAC 3 cut(s) 84, 533, 1011
RsaNI GTAC 3 cut(s) 83, 532, 1010
SaqAI TTAA 7 cut(s) 690, 972, 993, 1107, 1176, 1248, 1314
SatI GCNGC 8 cut(s) 227, 476, 678, 681, 760, 795, 836, 1344
Sau3AI GATC 3 cut(s) 118, 135, 292
Sau96I GGNCC 1 cut(s) 1333
ScaI AGTACT 1 cut(s) 533
SchI GAGTC 1 cut(s) 323
ScrFI CCNGG 1 cut(s) 1193
SduI GDGCHC 2 cut(s) 181, 243
SexAI ACCWGGT 1 cut(s) 1191
SfaNI GCATC 4 cut(s) 315, 523, 543, 555
SfcI CTRYAG 3 cut(s) 479, 678, 836
SmlI CTYRAG 2 cut(s) 509, 1328
SmoI CTYRAG 2 cut(s) 509, 1328
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 5 cut(s) 4, 546, 880, 954, 1240
SseBI AGGCCT 1 cut(s) 498
SsiI CCGC 1 cut(s) 1208
SspI AATATT 1 cut(s) 523
SspMI CTAG 6 cut(s) 102, 186, 204, 1085, 1214, 1393
StuI AGGCCT 1 cut(s) 498
StyD4I CCNGG 1 cut(s) 1191
StyI CCWWGG 2 cut(s) 656, 1368
TaaI ACNGT 1 cut(s) 94
TaiI ACGT 4 cut(s) 78, 88, 371, 917
TaqI TCGA 6 cut(s) 115, 121, 159, 279, 814, 948
TasI AATT 5 cut(s) 4, 546, 880, 954, 1240
TatI WGTACW 2 cut(s) 531, 1009
TfiI GAWTC 3 cut(s) 112, 207, 1029
Tru1I TTAA 7 cut(s) 690, 972, 993, 1107, 1176, 1248, 1314
Tru9I TTAA 7 cut(s) 690, 972, 993, 1107, 1176, 1248, 1314
TscAI CASTG 3 cut(s) 46, 99, 382
TseFI GTSAC 1 cut(s) 1159
TseI GCWGC 8 cut(s) 226, 475, 677, 680, 759, 794, 835, 1343
Tsp45I GTSAC 1 cut(s) 1159
TspDTI ATGAA 9 cut(s) 17, 437, 684, 713, 893, 920, 990, 1029, 1373
TspGWI ACGGA 1 cut(s) 233
TspRI CASTG 3 cut(s) 46, 99, 382
Tth111I GACNNNGTC 1 cut(s) 94
VneI GTGCAC 1 cut(s) 177
XapI RAATTY 3 cut(s) 546, 880, 1240
XcmI CCANNNNNNNNNTGG 1 cut(s) 1199
XmnI GAANNNNTTC 2 cut(s) 438, 1148
XspI CTAG 6 cut(s) 102, 186, 204, 1085, 1214, 1393
ZrmI AGTACT 1 cut(s) 533
Zsp2I ATGCAT 1 cut(s) 570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.