pycom15g03330

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0001271
Physical Location & Seq
Forward (+)
2060690 .. 2061685
996 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g03330.5

Sequence Viewer

Length: 510 bp
ATGACGGAATCTGTAGTTCGCAATTTCACTCGCCACATCCTCTCCGGGCTGGCCTTTCTGCATGGCACGAAGATAATTCACAGGGATATAAGGGGTGCAAATTTGCGTCTTGATGCATCAGGAGTTGTTAAAATTGTCAATTTTGGGATTGCAAAACACCTCAACAGGCAATCAGATAAAGTTTCGTTGAAGGGCAGTCTGAACTGGATGGCTCCTGAGGTCATAAGGACTGCAATGCAGAACAACGTGGATCCTGATTTATCTTTGGCCGTTGATATATGGAGCTTGGGGTGTACCATCATTGAGATGTTCACTGGAAAACCTCCTTGGAGTGAATGTACAGGACCTCAAGTTATGTTCAAGATGCTGAACAGAATCCCAGATATTCCAGAAACATTGTCTGCAGAAGGAAAGGACTTCCTCCATTGGTGCTTTCGGAGGGACCCTGCCAAGAGACCATCGGCTGCCGAGCTACTTGAACATCCATTTGTACATGCACAAAACCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04010 ko04011 ko04139 ko04722 ko04912 ko05166 map04010 map04011 map04139 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

170

Amino Acids

18.99

Weight (kDa)

8.91

Isoelectric Point (pI)

35.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 245, 258
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 1 cut(s) 100
AfaI GTAC 3 cut(s) 295, 340, 492
AgsI TTSAA 3 cut(s) 190, 361, 479
AjuI GAANNNNNNNTTGG 2 cut(s) 310, 342
AluBI AGCT 2 cut(s) 285, 472
AluI AGCT 2 cut(s) 285, 472
Alw26I GTCTC 1 cut(s) 448
AlwI GGATC 2 cut(s) 245, 258
AoxI GGCC 2 cut(s) 51, 267
ApeKI GCWGC 1 cut(s) 464
ApoI RAATTY 1 cut(s) 100
AspS9I GGNCC 2 cut(s) 344, 442
AsuC2I CCSGG 1 cut(s) 46
AvaII GGWCC 2 cut(s) 344, 442
AxyI CCTNAGG 1 cut(s) 216
BamHI GGATCC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 451
BccI CCATC 3 cut(s) 202, 305, 466
BceAI ACGGC 1 cut(s) 254
BcnI CCSGG 1 cut(s) 46
BcoDI GTCTC 1 cut(s) 448
BfmI CTRYAG 2 cut(s) 12, 402
BisI GCNGC 1 cut(s) 465
BlsI GCNGC 1 cut(s) 466
Bme1390I CCNGG 1 cut(s) 46
Bme18I GGWCC 2 cut(s) 344, 442
BmgT120I GGNCC 2 cut(s) 344, 442
BmiI GGNNCC 4 cut(s) 213, 252, 443, 444
BmrFI CCNGG 1 cut(s) 46
BmsI GCATC 3 cut(s) 103, 125, 354
BpuEI CTTGAG 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 46
BsaI GGTCTC 1 cut(s) 448
BsaJI CCNNGG 1 cut(s) 326
BsaXI ACNNNNNCTCC 4 cut(s) 26, 56, 322, 352
Bse1I ACTGG 2 cut(s) 209, 319
Bse21I CCTNAGG 1 cut(s) 216
Bse3DI GCAATG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 326
BseGI GGATG 3 cut(s) 36, 213, 481
BseMI GCAATG 1 cut(s) 240
BseMII CTCAG 1 cut(s) 207
BseNI ACTGG 2 cut(s) 209, 319
BseXI GCAGC 1 cut(s) 451
BshFI GGCC 2 cut(s) 53, 269
BsiSI CCGG 1 cut(s) 45
BslFI GGGAC 1 cut(s) 455
BsmAI GTCTC 1 cut(s) 448
BsmFI GGGAC 1 cut(s) 455
BsnI GGCC 2 cut(s) 53, 269
Bso31I GGTCTC 1 cut(s) 448
Bsp1407I TGTACA 2 cut(s) 338, 490
Bsp143I GATC 1 cut(s) 250
BspANI GGCC 2 cut(s) 53, 269
BspCNI CTCAG 1 cut(s) 208
BspLI GGNNCC 4 cut(s) 213, 252, 443, 444
BspMAI CTGCAG 1 cut(s) 406
BspPI GGATC 2 cut(s) 245, 258
BspTNI GGTCTC 1 cut(s) 448
BsrDI GCAATG 1 cut(s) 240
BsrGI TGTACA 2 cut(s) 338, 490
BsrI ACTGG 2 cut(s) 209, 319
BssECI CCNNGG 1 cut(s) 326
BssMI GATC 1 cut(s) 250
BssT1I CCWWGG 1 cut(s) 326
BstAUI TGTACA 2 cut(s) 338, 490
BstC8I GCNNGC 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 216
BstF5I GGATG 3 cut(s) 36, 213, 481
BstKTI GATC 1 cut(s) 253
BstMAI GTCTC 1 cut(s) 448
BstMBI GATC 1 cut(s) 250
BstNSI RCATGY 1 cut(s) 497
BstSCI CCNGG 1 cut(s) 44
BstSFI CTRYAG 2 cut(s) 12, 402
BstV1I GCAGC 1 cut(s) 451
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
Bsu36I CCTNAGG 1 cut(s) 216
BsuRI GGCC 2 cut(s) 53, 269
BtsCI GGATG 3 cut(s) 36, 213, 481
BtsIMutI CAGTG 1 cut(s) 312
Cac8I GCNNGC 1 cut(s) 51
Cfr13I GGNCC 2 cut(s) 344, 442
CseI GACGC 1 cut(s) 95
Csp6I GTAC 3 cut(s) 294, 339, 491
CviAII CATG 2 cut(s) 62, 494
CviJI RGCY 7 cut(s) 49, 53, 212, 269, 285, 464, 472
CviKI_1 RGCY 7 cut(s) 49, 53, 212, 269, 285, 464, 472
CviQI GTAC 3 cut(s) 294, 339, 491
DdeI CTNAG 1 cut(s) 216
DpnI GATC 1 cut(s) 252
DpnII GATC 1 cut(s) 250
EaeI YGGCCR 1 cut(s) 267
Eco130I CCWWGG 1 cut(s) 326
Eco31I GGTCTC 1 cut(s) 448
Eco47I GGWCC 2 cut(s) 344, 442
Eco81I CCTNAGG 1 cut(s) 216
EcoO109I RGGNCCY 2 cut(s) 344, 442
EcoT14I CCWWGG 1 cut(s) 326
EcoT22I ATGCAT 1 cut(s) 118
ErhI CCWWGG 1 cut(s) 326
FaeI CATG 2 cut(s) 65, 497
FaiI YATR 7 cut(s) 63, 89, 224, 278, 280, 356, 495
FaqI GGGAC 1 cut(s) 455
FatI CATG 2 cut(s) 61, 493
Fnu4HI GCNGC 1 cut(s) 465
FokI GGATG 3 cut(s) 23, 220, 468
Fsp4HI GCNGC 1 cut(s) 465
GluI GCNGC 1 cut(s) 465
HaeIII GGCC 2 cut(s) 53, 269
HapII CCGG 1 cut(s) 45
HgaI GACGC 1 cut(s) 95
Hin1II CATG 2 cut(s) 65, 497
HinfI GANTC 2 cut(s) 8, 375
HpaII CCGG 1 cut(s) 45
Hpy166II GTNNAC 2 cut(s) 294, 312
Hpy188I TCNGA 3 cut(s) 175, 201, 438
Hpy188III TCNNGA 6 cut(s) 110, 120, 215, 254, 361, 389
Hpy8I GTNNAC 2 cut(s) 294, 312
HpyAV CCTTC 2 cut(s) 184, 401
HpyCH4IV ACGT 1 cut(s) 246
HpyCH4V TGCA 8 cut(s) 61, 98, 116, 152, 233, 238, 404, 497
HpyF3I CTNAG 1 cut(s) 216
HpySE526I ACGT 1 cut(s) 246
Hsp92II CATG 2 cut(s) 65, 497
KflI GGGWCCC 1 cut(s) 442
Kzo9I GATC 1 cut(s) 250
LmnI GCTCC 2 cut(s) 217, 282
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 3 cut(s) 103, 125, 354
MaeII ACGT 1 cut(s) 246
MalI GATC 1 cut(s) 252
MboI GATC 1 cut(s) 250
MboII GAAGA 1 cut(s) 82
MflI RGATCY 1 cut(s) 250
MluCI AATT 5 cut(s) 22, 75, 100, 132, 139
MnlI CCTC 7 cut(s) 50, 170, 211, 333, 357, 431, 432
Mph1103I ATGCAT 1 cut(s) 118
MseI TTAA 1 cut(s) 129
MslI CAYNNNNRTG 1 cut(s) 305
MspI CCGG 1 cut(s) 45
MspR9I CCNGG 1 cut(s) 46
NciI CCSGG 1 cut(s) 46
NdeII GATC 1 cut(s) 250
NlaIII CATG 2 cut(s) 65, 497
NlaIV GGNNCC 4 cut(s) 213, 252, 443, 444
NmeAIII GCCGAG 1 cut(s) 493
NsiI ATGCAT 1 cut(s) 118
NspI RCATGY 1 cut(s) 497
PfeI GAWTC 2 cut(s) 8, 375
PkrI GCNGC 1 cut(s) 466
PpuMI RGGWCCY 2 cut(s) 344, 442
Psp5II RGGWCCY 2 cut(s) 344, 442
PspN4I GGNNCC 4 cut(s) 213, 252, 443, 444
PspPI GGNCC 2 cut(s) 344, 442
PspPPI RGGWCCY 2 cut(s) 344, 442
PstI CTGCAG 1 cut(s) 406
PsuI RGATCY 1 cut(s) 250
RsaI GTAC 3 cut(s) 295, 340, 492
RsaNI GTAC 3 cut(s) 294, 339, 491
RseI CAYNNNNRTG 1 cut(s) 305
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 1 cut(s) 465
Sau3AI GATC 1 cut(s) 250
Sau96I GGNCC 2 cut(s) 344, 442
ScrFI CCNGG 1 cut(s) 46
SetI ASST 7 cut(s) 162, 222, 249, 287, 325, 349, 474
SfaNI GCATC 3 cut(s) 103, 125, 354
SfcI CTRYAG 2 cut(s) 12, 402
SinI GGWCC 2 cut(s) 344, 442
SmiMI CAYNNNNRTG 1 cut(s) 305
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
Sse9I AATT 5 cut(s) 22, 75, 100, 132, 139
StyD4I CCNGG 1 cut(s) 44
StyI CCWWGG 1 cut(s) 326
TaiI ACGT 1 cut(s) 249
TasI AATT 5 cut(s) 22, 75, 100, 132, 139
TatI WGTACW 2 cut(s) 338, 490
TfiI GAWTC 2 cut(s) 8, 375
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TscAI CASTG 1 cut(s) 319
TseI GCWGC 1 cut(s) 464
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 1 cut(s) 319
VpaK11BI GGWCC 2 cut(s) 344, 442
XapI RAATTY 1 cut(s) 100
XceI RCATGY 1 cut(s) 497
Zsp2I ATGCAT 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.