Rmu_sc0003419.1_g000041

Mitogen-activated protein kinase kinase kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003419.1
OG0001271
Physical Location & Seq
Reverse (-)
142809 .. 145276
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003419.1_g000041.1.cds

Sequence Viewer

Length: 1068 bp
tctgttacctctgaagtccatccattgcctctgcctcctggagcaagcctcccttcacctagtgtaccatcaccacaacctgcgctcacacctaagccagagtcccagccgattaaagggcaatggcaaaagggaaagcttattggtcgtggcacctttggcagtgtttatgttgctaccaatagagaaactggagctttatgtgcaatgaaggaagttgaactacttcctgatgacccgaaatccgcagagtgtataaagcagctacagcaggaaatcaaagtcctcagccagctcaagcatcctaatattgtgcagtactatggaagtgaaattgttgaagatcggttttttatatatctggaatatgttcatcctggttctattaacaaatatgttcatgaacactgtggagccatgacagaagctgttgttcgcagttttactcgccatatagtctctgggttggcttacttgcacagcacaaaaacaatacacagggacatcaaaggggctaatttgcttgtggattcatgtggggttgtcaagctagctgactttggaatggccaaacatagggctatgcgtcctatgctttctgatactgatacagtttttatccaacagctgatgcaatctgagatgcataaagataacagctctgatttggctcttgctgttgatatttggagtttgggttgtactattattgaaatgttcacaggaaaacctccatggagtgagtatgaaggggctgcagctatgtttaaagttatgaaggatactccaacgtttccagaaccattgtcacctgagggtaaggacttccttaggtgctgcttccaaagaaatcctgctgagaggtcaactgctgcaatgctattagaacatcgatttataaaaaactcccagctgcaggaagtcccatctacgacctataatgcctctaacgggatgaatttcttggaaagaccccagactccaattgaacttgaaagaagacttgatagttcactgatgattcccagcattggcatgcaattaactacgggaaaggggcctctctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000165 GO:0000186 GO:0000187 GO:0001932 GO:0001934 GO:0002682 GO:0002831 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004709 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006979 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008152 GO:0009719 GO:0009893 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010200 GO:0010243 GO:0010562 GO:0010604 GO:0010646 GO:0010647 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019220 GO:0019222 GO:0019538 GO:0023014 GO:0023051 GO:0023052 GO:0023056 GO:0031098 GO:0031323 GO:0031325 GO:0031347 GO:0031399 GO:0031401 GO:0032101 GO:0032147 GO:0032268 GO:0032270 GO:0032879 GO:0033554 GO:0033674 GO:0035556 GO:0035690 GO:0036211 GO:0042221 GO:0042325 GO:0042327 GO:0042493 GO:0043085 GO:0043170 GO:0043405 GO:0043406 GO:0043408 GO:0043410 GO:0043412 GO:0043549 GO:0043900 GO:0044093 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045859 GO:0045860 GO:0045937 GO:0046777 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050776 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051246 GO:0051247 GO:0051338 GO:0051347 GO:0051716 GO:0051726 GO:0060255 GO:0065007 GO:0065009 GO:0070887 GO:0071310 GO:0071323 GO:0071417 GO:0071495 GO:0071704 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0080134 GO:0140096 GO:1900150 GO:1901564 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902531 GO:1902533 GO:2000071
KEGG Pathways
Metabolic & Signaling
ko04010 ko04722 ko04912 ko05166 map04010 map04722 map04912 map05166
Pfam Domains
Protein Families
Pkinase

Protein Analysis

355

Amino Acids

39.43

Weight (kDa)

6.6

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
Acc36I ACCTGC 1 cut(s) 88
AccB1I GGYRCC 1 cut(s) 152
AciI CCGC 1 cut(s) 246
AclI AACGTT 1 cut(s) 791
AcoI YGGCCR 1 cut(s) 567
AcsI RAATTY 1 cut(s) 958
AcuI CTGAAG 1 cut(s) 33
AdeI CACNNNGTG 1 cut(s) 62
AfaI GTAC 3 cut(s) 66, 320, 703
AfiI CCNNNNNNNGG 5 cut(s) 116, 576, 916, 951, 1031
AgsI TTSAA 5 cut(s) 221, 341, 713, 989, 995
AjnI CCWGG 2 cut(s) 37, 376
Alw26I GTCTC 1 cut(s) 463
AlwNI CAGNNNCTG 1 cut(s) 428
AoxI GGCC 2 cut(s) 567, 1058
ApeKI GCWGC 6 cut(s) 262, 755, 758, 837, 872, 913
ApoI RAATTY 1 cut(s) 958
Asp700I GAANNNNTTC 2 cut(s) 225, 369
AspLEI GCGC 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 1058
AsuHPI GGTGA 3 cut(s) 48, 63, 801
AsuNHI GCTAGC 1 cut(s) 550
AxyI CCTNAGG 2 cut(s) 813, 830
BalI TGGCCA 1 cut(s) 569
BanI GGYRCC 1 cut(s) 152
BarI GAAGNNNNNNTAC 2 cut(s) 207, 239
BbsI GAAGAC 1 cut(s) 1006
BbvCI CCTCAGC 1 cut(s) 287
BbvI GCAGC 6 cut(s) 274, 742, 770, 824, 859, 900
BccI CCATC 3 cut(s) 27, 76, 934
BciT130I CCWGG 2 cut(s) 39, 378
BciVI GTATCC 1 cut(s) 775
BcoDI GTCTC 1 cut(s) 463
BfaI CTAG 2 cut(s) 60, 551
BfmI CTRYAG 3 cut(s) 266, 756, 914
BfuAI ACCTGC 1 cut(s) 88
BfuI GTATCC 1 cut(s) 775
BisI GCNGC 6 cut(s) 263, 756, 759, 838, 873, 914
BlsI GCNGC 6 cut(s) 264, 757, 760, 839, 874, 915
BmcAI AGTACT 1 cut(s) 320
Bme1390I CCNGG 2 cut(s) 39, 378
BmgT120I GGNCC 1 cut(s) 1058
BmiI GGNNCC 3 cut(s) 154, 415, 1059
BmrFI CCNGG 2 cut(s) 39, 378
BmsI GCATC 3 cut(s) 310, 621, 633
BmtI GCTAGC 1 cut(s) 554
BpiI GAAGAC 1 cut(s) 1006
BplI GAGNNNNNCTC 2 cut(s) 33, 65
BpmI CTGGAG 2 cut(s) 60, 213
Bpu10I CCTNAGC 2 cut(s) 93, 287
BpuEI CTTGAG 1 cut(s) 281
Bsa29I ATCGAT 1 cut(s) 892
BsaJI CCNNGG 1 cut(s) 734
BsaXI ACNNNNNCTCC 2 cut(s) 682, 712
Bsc4I CCNNNNNNNGG 5 cut(s) 116, 576, 916, 951, 1031
Bse1I ACTGG 1 cut(s) 196
Bse21I CCTNAGG 2 cut(s) 813, 830
Bse3DI GCAATG 4 cut(s) 23, 128, 213, 882
BseBI CCWGG 2 cut(s) 39, 378
BseCI ATCGAT 1 cut(s) 892
BseDI CCNNGG 1 cut(s) 734
BseGI GGATG 4 cut(s) 19, 301, 373, 960
BseLI CCNNNNNNNGG 5 cut(s) 116, 576, 916, 951, 1031
BseMI GCAATG 4 cut(s) 23, 128, 213, 882
BseMII CTCAG 4 cut(s) 301, 630, 804, 849
BseNI ACTGG 1 cut(s) 196
BseXI GCAGC 6 cut(s) 274, 742, 770, 824, 859, 900
BseYI CCCAGC 3 cut(s) 105, 909, 1025
BsgI GTGCAG 1 cut(s) 335
BshFI GGCC 2 cut(s) 569, 1060
BshNI GGYRCC 1 cut(s) 152
BshVI ATCGAT 1 cut(s) 892
BslFI GGGAC 3 cut(s) 88, 515, 908
BslI CCNNNNNNNGG 5 cut(s) 116, 576, 916, 951, 1031
BsmAI GTCTC 1 cut(s) 463
BsmFI GGGAC 3 cut(s) 88, 515, 908
BsnI GGCC 2 cut(s) 569, 1060
Bsp143I GATC 1 cut(s) 343
Bsp19I CCATGG 1 cut(s) 734
BspACI CCGC 1 cut(s) 246
BspANI GGCC 2 cut(s) 569, 1060
BspCNI CTCAG 4 cut(s) 300, 631, 805, 850
BspDI ATCGAT 1 cut(s) 892
BspHI TCATGA 1 cut(s) 400
BspLI GGNNCC 3 cut(s) 154, 415, 1059
BspMAI CTGCAG 2 cut(s) 760, 918
BspMI ACCTGC 1 cut(s) 88
BspOI GCTAGC 1 cut(s) 554
BspT107I GGYRCC 1 cut(s) 152
BsrDI GCAATG 4 cut(s) 23, 128, 213, 882
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 1 cut(s) 734
BssMI GATC 1 cut(s) 343
BssT1I CCWWGG 1 cut(s) 734
Bst2UI CCWGG 2 cut(s) 39, 378
Bst4CI ACNGT 2 cut(s) 410, 613
BstC8I GCNNGC 4 cut(s) 46, 293, 552, 1037
BstDEI CTNAG 6 cut(s) 93, 287, 639, 813, 830, 858
BstDSI CCRYGG 1 cut(s) 734
BstF5I GGATG 4 cut(s) 19, 301, 373, 960
BstHHI GCGC 1 cut(s) 85
BstKTI GATC 1 cut(s) 346
BstMAI GTCTC 1 cut(s) 463
BstMBI GATC 1 cut(s) 343
BstMWI GCNNNNNNNGC 4 cut(s) 159, 203, 268, 592
BstNI CCWGG 2 cut(s) 39, 378
BstNSI RCATGY 1 cut(s) 1039
BstSCI CCNGG 2 cut(s) 37, 376
BstSFI CTRYAG 3 cut(s) 266, 756, 914
BstV1I GCAGC 6 cut(s) 274, 742, 770, 824, 859, 900
BstV2I GAAGAC 1 cut(s) 1006
Bsu15I ATCGAT 1 cut(s) 892
Bsu36I CCTNAGG 2 cut(s) 813, 830
BsuI GTATCC 1 cut(s) 775
BsuRI GGCC 2 cut(s) 569, 1060
BsuTUI ATCGAT 1 cut(s) 892
BtgI CCRYGG 1 cut(s) 734
BtsCI GGATG 4 cut(s) 19, 301, 373, 960
BtsI GCAGTG 1 cut(s) 169
BtsIMutI CAGTG 3 cut(s) 169, 406, 1013
BveI ACCTGC 1 cut(s) 88
Cac8I GCNNGC 4 cut(s) 46, 293, 552, 1037
CaiI CAGNNNCTG 1 cut(s) 428
CciI TCATGA 1 cut(s) 400
CfoI GCGC 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 1058
ClaI ATCGAT 1 cut(s) 892
CseI GACGC 1 cut(s) 575
Csp6I GTAC 3 cut(s) 65, 319, 702
CviAII CATG 5 cut(s) 401, 418, 534, 735, 1036
CviQI GTAC 3 cut(s) 65, 319, 702
DdeI CTNAG 6 cut(s) 93, 287, 639, 813, 830, 858
DpnI GATC 1 cut(s) 345
DpnII GATC 1 cut(s) 343
DraI TTTAAA 1 cut(s) 769
DraIII CACNNNGTG 1 cut(s) 62
EaeI YGGCCR 1 cut(s) 567
Eco130I CCWWGG 1 cut(s) 734
Eco57I CTGAAG 1 cut(s) 33
Eco81I CCTNAGG 2 cut(s) 813, 830
EcoO109I RGGNCCY 1 cut(s) 1058
EcoRII CCWGG 2 cut(s) 37, 376
EcoT14I CCWWGG 1 cut(s) 734
EcoT22I ATGCAT 1 cut(s) 648
ErhI CCWWGG 1 cut(s) 734
FaeI CATG 5 cut(s) 404, 421, 537, 738, 1039
FalI AAGNNNNNCTT 2 cut(s) 37, 69
FaqI GGGAC 3 cut(s) 88, 515, 908
FatI CATG 5 cut(s) 400, 417, 533, 734, 1035
Fnu4HI GCNGC 6 cut(s) 263, 756, 759, 838, 873, 914
FokI GGATG 4 cut(s) 6, 288, 360, 967
Fsp4HI GCNGC 6 cut(s) 263, 756, 759, 838, 873, 914
FspBI CTAG 2 cut(s) 60, 551
GlaI GCGC 1 cut(s) 84
GluI GCNGC 6 cut(s) 263, 756, 759, 838, 873, 914
GsaI CCCAGC 3 cut(s) 109, 913, 1029
GsuI CTGGAG 2 cut(s) 60, 213
HaeIII GGCC 2 cut(s) 569, 1060
HgaI GACGC 1 cut(s) 575
HhaI GCGC 1 cut(s) 85
Hin1II CATG 5 cut(s) 404, 421, 537, 738, 1039
Hin6I GCGC 1 cut(s) 83
HinP1I GCGC 1 cut(s) 83
HincII GTYRAC 1 cut(s) 867
HindII GTYRAC 1 cut(s) 867
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 4 cut(s) 101, 530, 979, 1021
HphI GGTGA 3 cut(s) 48, 63, 801
Hpy166II GTNNAC 4 cut(s) 65, 720, 867, 1013
Hpy188I TCNGA 5 cut(s) 13, 601, 640, 664, 1067
Hpy188III TCNNGA 4 cut(s) 230, 362, 401, 797
Hpy8I GTNNAC 4 cut(s) 65, 720, 867, 1013
HpyAV CCTTC 4 cut(s) 63, 205, 743, 772
HpyCH4III ACNGT 2 cut(s) 410, 613
HpyCH4IV ACGT 1 cut(s) 791
HpyCH4V TGCA 9 cut(s) 206, 316, 478, 634, 646, 758, 875, 916, 1039
HpyF10VI GCNNNNNNNGC 4 cut(s) 159, 203, 268, 592
HpyF3I CTNAG 6 cut(s) 93, 287, 639, 813, 830, 858
HpySE526I ACGT 1 cut(s) 791
Hsp92II CATG 5 cut(s) 404, 421, 537, 738, 1039
HspAI GCGC 1 cut(s) 83
Kzo9I GATC 1 cut(s) 343
LmnI GCTCC 3 cut(s) 41, 194, 413
Lsp1109I GCAGC 6 cut(s) 274, 742, 770, 824, 859, 900
LweI GCATC 3 cut(s) 310, 621, 633
MaeI CTAG 2 cut(s) 60, 551
MaeII ACGT 1 cut(s) 791
MaeIII GTNAC 2 cut(s) 4, 807
MalI GATC 1 cut(s) 345
MboI GATC 1 cut(s) 343
MboII GAAGA 2 cut(s) 353, 1011
MfeI CAATTG 1 cut(s) 984
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 5 cut(s) 333, 517, 958, 984, 1040
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 2 cut(s) 110, 973
MmeI TCCRAC 2 cut(s) 646, 812
MnlI CCTC 9 cut(s) 19, 39, 45, 59, 296, 741, 808, 855, 955
Mox20I TGGCCA 1 cut(s) 569
Mph1103I ATGCAT 1 cut(s) 648
MroXI GAANNNNTTC 2 cut(s) 225, 369
MscI TGGCCA 1 cut(s) 569
MseI TTAA 4 cut(s) 114, 387, 768, 1043
MslI CAYNNNNRTG 1 cut(s) 1034
Msp20I TGGCCA 1 cut(s) 569
MspA1I CMGCKG 2 cut(s) 628, 913
MspR9I CCNGG 2 cut(s) 39, 378
MunI CAATTG 1 cut(s) 984
MvaI CCWGG 2 cut(s) 39, 378
MwoI GCNNNNNNNGC 4 cut(s) 159, 203, 268, 592
NcoI CCATGG 1 cut(s) 734
NdeII GATC 1 cut(s) 343
NheI GCTAGC 1 cut(s) 550
NlaIII CATG 5 cut(s) 404, 421, 537, 738, 1039
NlaIV GGNNCC 3 cut(s) 154, 415, 1059
NmuCI GTSAC 1 cut(s) 807
NsiI ATGCAT 1 cut(s) 648
NspI RCATGY 1 cut(s) 1039
PaeI GCATGC 1 cut(s) 1039
PagI TCATGA 1 cut(s) 400
PdmI GAANNNNTTC 2 cut(s) 225, 369
PfeI GAWTC 2 cut(s) 530, 1021
PfoI TCCNGGA 1 cut(s) 37
PkrI GCNGC 6 cut(s) 264, 757, 760, 839, 874, 915
PleI GAGTC 2 cut(s) 109, 973
PpsI GAGTC 2 cut(s) 109, 973
PsiI TTATAA 1 cut(s) 899
Psp1406I AACGTT 1 cut(s) 791
Psp6I CCWGG 2 cut(s) 37, 376
PspFI CCCAGC 3 cut(s) 105, 909, 1025
PspGI CCWGG 2 cut(s) 37, 376
PspN4I GGNNCC 3 cut(s) 154, 415, 1059
PspPI GGNCC 1 cut(s) 1058
PstI CTGCAG 2 cut(s) 760, 918
PstNI CAGNNNCTG 1 cut(s) 428
PvuII CAGCTG 2 cut(s) 628, 913
RsaI GTAC 3 cut(s) 66, 320, 703
RsaNI GTAC 3 cut(s) 65, 319, 702
RseI CAYNNNNRTG 1 cut(s) 1034
SaqAI TTAA 4 cut(s) 114, 387, 768, 1043
SatI GCNGC 6 cut(s) 263, 756, 759, 838, 873, 914
Sau3AI GATC 1 cut(s) 343
Sau96I GGNCC 1 cut(s) 1058
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 2 cut(s) 110, 973
ScrFI CCNGG 2 cut(s) 39, 378
SfaNI GCATC 3 cut(s) 310, 621, 633
SfcI CTRYAG 3 cut(s) 266, 756, 914
SmiMI CAYNNNNRTG 1 cut(s) 1034
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SphI GCATGC 1 cut(s) 1039
Sse9I AATT 5 cut(s) 333, 517, 958, 984, 1040
SsiI CCGC 1 cut(s) 246
SspI AATATT 1 cut(s) 310
SspMI CTAG 2 cut(s) 60, 551
StyD4I CCNGG 2 cut(s) 37, 376
StyI CCWWGG 1 cut(s) 734
TaaI ACNGT 2 cut(s) 410, 613
TaiI ACGT 1 cut(s) 794
TaqI TCGA 1 cut(s) 892
TasI AATT 5 cut(s) 333, 517, 958, 984, 1040
TatI WGTACW 2 cut(s) 318, 701
TfiI GAWTC 2 cut(s) 530, 1021
Tru1I TTAA 4 cut(s) 114, 387, 768, 1043
Tru9I TTAA 4 cut(s) 114, 387, 768, 1043
TscAI CASTG 3 cut(s) 169, 413, 1020
TseFI GTSAC 1 cut(s) 807
TseI GCWGC 6 cut(s) 262, 755, 758, 837, 872, 913
Tsp45I GTSAC 1 cut(s) 807
TspDTI ATGAA 8 cut(s) 224, 362, 389, 417, 522, 762, 791, 971
TspRI CASTG 3 cut(s) 169, 413, 1020
XapI RAATTY 1 cut(s) 958
XceI RCATGY 1 cut(s) 1039
XcmI CCANNNNNNNNNTGG 1 cut(s) 458
XmnI GAANNNNTTC 2 cut(s) 225, 369
XspI CTAG 2 cut(s) 60, 551
ZrmI AGTACT 1 cut(s) 320
Zsp2I ATGCAT 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.