FvH4_1g23820

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000532
Physical Location & Seq
Forward (+)
15700031 .. 15700818
788 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23820.t1

Sequence Viewer

Length: 690 bp
ATGGCACACTCCTTTCACTCCCTAGCTTTCTTCTGCTCAAGCATTCTAATATTCAAGCTCATCATTCTTCACCAGTCAGCCTTGGCTTGGAGTTGGGGACCTGATGTTCCTCCTACACAAGGCTTTGTCTCCCTCCCTTTCAACCGATCCTACTACCATATTCAGAAGCCTTACGATGTGCCAGAAGACCAGCGCTACTGCTTCATCGATGGAGTTCATAAGTGTTGGGTCTACTCCACAGACAAACCTCACACTACTACCAGCCAAACCCTGCCTCGCACTGAGATTGCCATACAAGGATACAACTACTCTTCTGGGGTGTGGGAGTTTGAAGCATATGGGTATGTACCAAAAGGGACATCAGGGGTTTGCATAATGCAAGTGTTTGGAGCAAGTGCTCCTTATGCCACAACTCTGATGCTGAGGGTCTACAATGGTTCACTAACATACTACACAGCTCCGGTGCTGGTTCCCAACATCTACGATAAGTGGTTCAAGCTCAATGTGGTTCATGATGTTGATGCTGCAAAAGTCCGAGTGTACATTGATGGATGTCTCAAAATCGAAGCAGATGGCCGTGGAGGAACCTCTCATGCTTTCAAATGTGGTGTCTATGCCCAGATGAATGACTCCTGTTACATGGAGTCTCGTTGGAAACACATCAAAATTTTAAGGAAGTGTGGACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

230

Amino Acids

26.04

Weight (kDa)

8.69

Isoelectric Point (pI)

43.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 50 - 223 7.2e-26 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 231, 429
AclWI GGATC 1 cut(s) 141
AcoI YGGCCR 1 cut(s) 574
AcsI RAATTY 1 cut(s) 666
AfaI GTAC 2 cut(s) 348, 542
AfeI AGCGCT 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 87, 119
AgsI TTSAA 5 cut(s) 55, 142, 332, 496, 601
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 4 cut(s) 26, 58, 458, 499
AluI AGCT 4 cut(s) 26, 58, 458, 499
Alw21I GWGCWC 1 cut(s) 400
Alw26I GTCTC 3 cut(s) 133, 560, 651
AlwI GGATC 1 cut(s) 141
Aor51HI AGCGCT 1 cut(s) 194
AoxI GGCC 1 cut(s) 574
ApeKI GCWGC 1 cut(s) 524
ApoI RAATTY 1 cut(s) 666
ArsI GACNNNNNNTTYG 2 cut(s) 594, 626
AspLEI GCGC 1 cut(s) 195
AspS9I GGNCC 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 62
AvaII GGWCC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 400
BbvCI CCTCAGC 1 cut(s) 422
BbvI GCAGC 1 cut(s) 511
BccI CCATC 3 cut(s) 203, 542, 566
BceAI ACGGC 1 cut(s) 561
BciVI GTATCC 1 cut(s) 293
BcoDI GTCTC 3 cut(s) 133, 560, 651
BfaI CTAG 1 cut(s) 23
BfoI RGCGCY 1 cut(s) 196
BfuI GTATCC 1 cut(s) 293
BisI GCNGC 1 cut(s) 525
BlsI GCNGC 1 cut(s) 526
Bme18I GGWCC 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 98
BmiI GGNNCC 3 cut(s) 99, 471, 586
BmsI GCATC 2 cut(s) 408, 511
BpiI GAAGAC 1 cut(s) 192
Bpu10I CCTNAGC 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 207
BsaJI CCNNGG 2 cut(s) 81, 577
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 119
Bse1I ACTGG 1 cut(s) 73
BseCI ATCGAT 1 cut(s) 207
BseDI CCNNGG 2 cut(s) 81, 577
BseGI GGATG 1 cut(s) 557
BseLI CCNNNNNNNGG 2 cut(s) 87, 119
BseMII CTCAG 2 cut(s) 273, 413
BseNI ACTGG 1 cut(s) 73
BseXI GCAGC 1 cut(s) 511
BshFI GGCC 1 cut(s) 576
BshVI ATCGAT 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 400
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 2 cut(s) 111, 370
BslI CCNNNNNNNGG 2 cut(s) 87, 119
BsmAI GTCTC 3 cut(s) 133, 560, 651
BsmFI GGGAC 2 cut(s) 111, 370
BsmI GAATGC 1 cut(s) 42
BsnI GGCC 1 cut(s) 576
Bsp1286I GDGCHC 1 cut(s) 400
Bsp1407I TGTACA 1 cut(s) 540
Bsp143I GATC 1 cut(s) 146
BspANI GGCC 1 cut(s) 576
BspCNI CTCAG 2 cut(s) 274, 414
BspDI ATCGAT 1 cut(s) 207
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 99, 471, 586
BspPI GGATC 1 cut(s) 141
BsrGI TGTACA 1 cut(s) 540
BsrI ACTGG 1 cut(s) 73
BssECI CCNNGG 2 cut(s) 81, 577
BssMI GATC 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 81
Bst6I CTCTTC 1 cut(s) 316
BstAUI TGTACA 1 cut(s) 540
BstDEI CTNAG 2 cut(s) 282, 422
BstDSI CCRYGG 1 cut(s) 577
BstENI CCTNNNNNAGG 1 cut(s) 117
BstF5I GGATG 1 cut(s) 557
BstH2I RGCGCY 1 cut(s) 196
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 1 cut(s) 149
BstMAI GTCTC 3 cut(s) 133, 560, 651
BstMBI GATC 1 cut(s) 146
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstV1I GCAGC 1 cut(s) 511
BstV2I GAAGAC 1 cut(s) 192
Bsu15I ATCGAT 1 cut(s) 207
BsuI GTATCC 1 cut(s) 293
BsuRI GGCC 1 cut(s) 576
BsuTUI ATCGAT 1 cut(s) 207
BtgI CCRYGG 1 cut(s) 577
BtsCI GGATG 1 cut(s) 557
BtsIMutI CAGTG 1 cut(s) 279
CciI TCATGA 1 cut(s) 511
CfoI GCGC 1 cut(s) 195
Cfr13I GGNCC 1 cut(s) 98
ClaI ATCGAT 1 cut(s) 207
Csp6I GTAC 2 cut(s) 347, 541
CviAII CATG 3 cut(s) 512, 593, 640
CviQI GTAC 2 cut(s) 347, 541
DdeI CTNAG 2 cut(s) 282, 422
DpnI GATC 1 cut(s) 148
DpnII GATC 1 cut(s) 146
EaeI YGGCCR 1 cut(s) 574
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Eco130I CCWWGG 1 cut(s) 81
Eco47I GGWCC 1 cut(s) 98
Eco47III AGCGCT 1 cut(s) 194
EcoNI CCTNNNNNAGG 1 cut(s) 117
EcoO109I RGGNCCY 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 81
ErhI CCWWGG 1 cut(s) 81
FaeI CATG 3 cut(s) 515, 596, 643
FalI AAGNNNNNCTT 2 cut(s) 385, 417
FaqI GGGAC 2 cut(s) 111, 370
FatI CATG 3 cut(s) 511, 592, 639
FauNDI CATATG 1 cut(s) 337
FblI GTMKAC 2 cut(s) 231, 429
Fnu4HI GCNGC 1 cut(s) 525
FokI GGATG 1 cut(s) 564
Fsp4HI GCNGC 1 cut(s) 525
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 194
GluI GCNGC 1 cut(s) 525
HaeII RGCGCY 1 cut(s) 196
HaeIII GGCC 1 cut(s) 576
HapII CCGG 1 cut(s) 461
HhaI GCGC 1 cut(s) 195
Hin1II CATG 3 cut(s) 515, 596, 643
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HinfI GANTC 2 cut(s) 629, 644
HpaII CCGG 1 cut(s) 461
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 5 cut(s) 232, 430, 440, 541, 683
Hpy188I TCNGA 3 cut(s) 165, 417, 536
Hpy188III TCNNGA 1 cut(s) 512
Hpy8I GTNNAC 5 cut(s) 232, 430, 440, 541, 683
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 3 cut(s) 372, 379, 527
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpyF3I CTNAG 2 cut(s) 282, 422
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 3 cut(s) 515, 596, 643
HspAI GCGC 1 cut(s) 193
Kzo9I GATC 1 cut(s) 146
LmnI GCTCC 3 cut(s) 389, 403, 463
Lsp1109I GCAGC 1 cut(s) 511
LweI GCATC 2 cut(s) 408, 511
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 1 cut(s) 635
MalI GATC 1 cut(s) 148
MboI GATC 1 cut(s) 146
MboII GAAGA 4 cut(s) 22, 59, 197, 303
MhlI GDGCHC 1 cut(s) 400
MluCI AATT 1 cut(s) 666
MlyI GAGTC 2 cut(s) 623, 653
MmeI TCCRAC 1 cut(s) 632
MnlI CCTC 7 cut(s) 120, 143, 258, 285, 417, 575, 598
MseI TTAA 2 cut(s) 671, 688
MspI CCGG 1 cut(s) 461
Mva1269I GAATGC 1 cut(s) 42
MwoI GCNNNNNNNGC 1 cut(s) 404
NdeI CATATG 1 cut(s) 337
NdeII GATC 1 cut(s) 146
NlaIII CATG 3 cut(s) 515, 596, 643
NlaIV GGNNCC 3 cut(s) 99, 471, 586
PagI TCATGA 1 cut(s) 511
PctI GAATGC 1 cut(s) 42
PkrI GCNGC 1 cut(s) 526
PleI GAGTC 2 cut(s) 623, 652
PpsI GAGTC 2 cut(s) 623, 652
PpuMI RGGWCCY 1 cut(s) 98
Psp5II RGGWCCY 1 cut(s) 98
PspN4I GGNNCC 3 cut(s) 99, 471, 586
PspPI GGNCC 1 cut(s) 98
PspPPI RGGWCCY 1 cut(s) 98
RsaI GTAC 2 cut(s) 348, 542
RsaNI GTAC 2 cut(s) 347, 541
SaqAI TTAA 2 cut(s) 671, 688
SatI GCNGC 1 cut(s) 525
Sau3AI GATC 1 cut(s) 146
Sau96I GGNCC 1 cut(s) 98
SchI GAGTC 2 cut(s) 623, 653
SduI GDGCHC 1 cut(s) 400
SetI ASST 8 cut(s) 28, 60, 103, 250, 460, 501, 590, 688
SfaNI GCATC 2 cut(s) 408, 511
SinI GGWCC 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 37
SmoI CTYRAG 1 cut(s) 37
Sse9I AATT 1 cut(s) 666
SspI AATATT 1 cut(s) 51
SspMI CTAG 1 cut(s) 23
StyI CCWWGG 1 cut(s) 81
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 207, 564
TasI AATT 1 cut(s) 666
TatI WGTACW 1 cut(s) 540
Tru1I TTAA 2 cut(s) 671, 688
Tru9I TTAA 2 cut(s) 671, 688
TscAI CASTG 1 cut(s) 286
TseI GCWGC 1 cut(s) 524
TspDTI ATGAA 4 cut(s) 193, 206, 500, 638
TspRI CASTG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 98
XagI CCTNNNNNAGG 1 cut(s) 117
XapI RAATTY 1 cut(s) 666
XmiI GTMKAC 2 cut(s) 231, 429
XspI CTAG 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.