Prupe.8G032800_v2.0.a1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000532
Physical Location & Seq
Reverse (-)
3082615 .. 3083590
976 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G032800.1

Sequence Viewer

Length: 669 bp
ATGTTGCCTTTAAATTGCATTAAAATGAATTCCTTCTTCGGCAAGCTCCTGTGCTTGATCCTATTGATCTTGAAGTACCGCTATATTTGTGCCACTGATCCTACTGATGGATTCACCCTTGTGCCACTAACAGAAGAGAACTTCAAGTTACAGAAACCCTACAACGAACCCCTCGATGATCGTTACAGTGACAAGGATGGAGTTCGAAGTTTCTGGATCTACAACCATGACAAGCCATTCAAAACCGATAGCACAACTAGGCCCCGTTCGGAAGCGCGCATAACGGGTCATGACTATTCTTCTGGGATTTGGCAATTCGAAGGCTATGCATTTGTGCCGAGTGGTACTTCTGGGGTTACAATAGTGCAGATCCATGGTGCAGCTGAGGGAGCTACGTCTCTACAATTAAGGATGTATGATGGAGATATCAAATACTACAAATACAACGTAGTAGCTACCAATCTCTATGATAAGTGGTTCAGAGTAAACATAATCCACAATGTTGACAAAGGGAAGATAATAGTTTTCATCGATGGTGTCAAAAAGTTTGTGGTGAAAGATCAGGGACCAGGGGACCTGTATTTCAAATGTGGAGTATATGCTGCACCAGAAAACTCGAGCAACTACATGGAATCACGGTGGAAAGAGATCAAACTTTACAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

223

Amino Acids

25.61

Weight (kDa)

8.84

Isoelectric Point (pI)

34.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 277
AciI CCGC 1 cut(s) 79
AclWI GGATC 4 cut(s) 52, 92, 224, 364
AcsI RAATTY 1 cut(s) 28
AfaI GTAC 2 cut(s) 77, 346
AfiI CCNNNNNNNGG 1 cut(s) 107
AgsI TTSAA 4 cut(s) 73, 145, 241, 586
AjnI CCWGG 1 cut(s) 568
AleI CACNNNNGTG 1 cut(s) 119
AluBI AGCT 4 cut(s) 46, 383, 392, 455
AluI AGCT 4 cut(s) 46, 383, 392, 455
Alw26I GTCTC 1 cut(s) 402
AlwI GGATC 4 cut(s) 52, 92, 224, 364
Ama87I CYCGRG 1 cut(s) 616
AoxI GGCC 1 cut(s) 260
ApeKI GCWGC 2 cut(s) 380, 602
ApoI RAATTY 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 32
AspLEI GCGC 2 cut(s) 277, 279
AspS9I GGNCC 3 cut(s) 261, 566, 574
AsuHPI GGTGA 2 cut(s) 106, 565
AsuII TTCGAA 2 cut(s) 205, 318
AvaI CYCGRG 1 cut(s) 616
AvaII GGWCC 2 cut(s) 566, 574
BbvCI CCTCAGC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 392, 589
BccI CCATC 4 cut(s) 101, 191, 413, 527
BcgI CGANNNNNNTGC 2 cut(s) 308, 342
BciT130I CCWGG 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 402
BfaI CTAG 1 cut(s) 258
BisI GCNGC 2 cut(s) 381, 603
BlsI GCNGC 2 cut(s) 382, 604
Bme1390I CCNGG 1 cut(s) 570
Bme18I GGWCC 2 cut(s) 566, 574
BmeT110I CYCGRG 1 cut(s) 616
BmgT120I GGNCC 3 cut(s) 261, 566, 574
BmiI GGNNCC 3 cut(s) 263, 567, 575
BmrFI CCNGG 1 cut(s) 570
Bpu10I CCTNAGC 1 cut(s) 384
Bpu14I TTCGAA 2 cut(s) 205, 318
Bsa29I ATCGAT 1 cut(s) 531
BsaJI CCNNGG 2 cut(s) 373, 569
Bsc4I CCNNNNNNNGG 1 cut(s) 107
BseBI CCWGG 1 cut(s) 570
BseCI ATCGAT 1 cut(s) 531
BseDI CCNNGG 2 cut(s) 373, 569
BseGI GGATG 2 cut(s) 202, 417
BseLI CCNNNNNNNGG 1 cut(s) 107
BseMII CTCAG 1 cut(s) 375
BsePI GCGCGC 1 cut(s) 275
BseXI GCAGC 2 cut(s) 392, 589
BsgI GTGCAG 3 cut(s) 386, 399, 588
Bsh1236I CGCG 1 cut(s) 277
BshFI GGCC 1 cut(s) 262
BshVI ATCGAT 1 cut(s) 531
BsiHKCI CYCGRG 1 cut(s) 616
BslFI GGGAC 2 cut(s) 579, 587
BslI CCNNNNNNNGG 1 cut(s) 107
BsmAI GTCTC 1 cut(s) 402
BsmBI CGTCTC 1 cut(s) 402
BsmFI GGGAC 2 cut(s) 579, 587
BsnI GGCC 1 cut(s) 262
BsoBI CYCGRG 1 cut(s) 616
Bsp119I TTCGAA 2 cut(s) 205, 318
Bsp143I GATC 8 cut(s) 57, 66, 97, 178, 216, 369, 559, 648
Bsp19I CCATGG 1 cut(s) 373
BspACI CCGC 1 cut(s) 79
BspANI GGCC 1 cut(s) 262
BspCNI CTCAG 1 cut(s) 376
BspDI ATCGAT 1 cut(s) 531
BspFNI CGCG 1 cut(s) 277
BspHI TCATGA 1 cut(s) 289
BspLI GGNNCC 3 cut(s) 263, 567, 575
BspPI GGATC 4 cut(s) 52, 92, 224, 364
BspT104I TTCGAA 2 cut(s) 205, 318
BssECI CCNNGG 2 cut(s) 373, 569
BssHII GCGCGC 1 cut(s) 275
BssMI GATC 8 cut(s) 57, 66, 97, 178, 216, 369, 559, 648
BssT1I CCWWGG 1 cut(s) 373
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 2 cut(s) 188, 639
Bst6I CTCTTC 1 cut(s) 129
BstBI TTCGAA 2 cut(s) 205, 318
BstC8I GCNNGC 2 cut(s) 44, 277
BstDEI CTNAG 1 cut(s) 384
BstDSI CCRYGG 1 cut(s) 373
BstF5I GGATG 2 cut(s) 202, 417
BstFNI CGCG 1 cut(s) 277
BstHHI GCGC 2 cut(s) 277, 279
BstKTI GATC 8 cut(s) 60, 69, 100, 181, 219, 372, 562, 651
BstMAI GTCTC 1 cut(s) 402
BstMBI GATC 8 cut(s) 57, 66, 97, 178, 216, 369, 559, 648
BstMWI GCNNNNNNNGC 1 cut(s) 389
BstNI CCWGG 1 cut(s) 570
BstSCI CCNGG 1 cut(s) 568
BstUI CGCG 1 cut(s) 277
BstV1I GCAGC 2 cut(s) 392, 589
BstX2I RGATCY 2 cut(s) 216, 369
BstYI RGATCY 2 cut(s) 216, 369
Bsu15I ATCGAT 1 cut(s) 531
BsuRI GGCC 1 cut(s) 262
BsuTUI ATCGAT 1 cut(s) 531
BtgI CCRYGG 1 cut(s) 373
BtsCI GGATG 2 cut(s) 202, 417
BtsIMutI CAGTG 2 cut(s) 93, 193
Cac8I GCNNGC 2 cut(s) 44, 277
CciI TCATGA 1 cut(s) 289
CfoI GCGC 2 cut(s) 277, 279
Cfr13I GGNCC 3 cut(s) 261, 566, 574
ClaI ATCGAT 1 cut(s) 531
Csp6I GTAC 2 cut(s) 76, 345
CviAII CATG 4 cut(s) 227, 290, 374, 628
CviJI RGCY 7 cut(s) 46, 235, 262, 324, 383, 392, 455
CviKI_1 RGCY 7 cut(s) 46, 235, 262, 324, 383, 392, 455
CviQI GTAC 2 cut(s) 76, 345
DdeI CTNAG 1 cut(s) 384
DpnI GATC 8 cut(s) 59, 68, 99, 180, 218, 371, 561, 650
DpnII GATC 8 cut(s) 57, 66, 97, 178, 216, 369, 559, 648
DraI TTTAAA 1 cut(s) 12
Eam1104I CTCTTC 1 cut(s) 129
EarI CTCTTC 1 cut(s) 129
Eco130I CCWWGG 1 cut(s) 373
Eco32I GATATC 1 cut(s) 427
Eco47I GGWCC 2 cut(s) 566, 574
Eco88I CYCGRG 1 cut(s) 616
EcoO109I RGGNCCY 2 cut(s) 261, 574
EcoRI GAATTC 1 cut(s) 28
EcoRII CCWGG 1 cut(s) 568
EcoRV GATATC 1 cut(s) 427
EcoT14I CCWWGG 1 cut(s) 373
EcoT22I ATGCAT 1 cut(s) 331
ErhI CCWWGG 1 cut(s) 373
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 4 cut(s) 230, 293, 377, 631
FaqI GGGAC 2 cut(s) 579, 587
FatI CATG 4 cut(s) 226, 289, 373, 627
Fnu4HI GCNGC 2 cut(s) 381, 603
FokI GGATG 2 cut(s) 209, 424
Fsp4HI GCNGC 2 cut(s) 381, 603
FspBI CTAG 1 cut(s) 258
GlaI GCGC 2 cut(s) 276, 278
GluI GCNGC 2 cut(s) 381, 603
HaeIII GGCC 1 cut(s) 262
HhaI GCGC 2 cut(s) 277, 279
Hin1II CATG 4 cut(s) 230, 293, 377, 631
Hin6I GCGC 2 cut(s) 275, 277
HinP1I GCGC 2 cut(s) 275, 277
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 2 cut(s) 111, 632
HphI GGTGA 2 cut(s) 106, 565
Hpy166II GTNNAC 2 cut(s) 487, 505
Hpy188I TCNGA 2 cut(s) 271, 482
Hpy188III TCNNGA 3 cut(s) 70, 214, 290
Hpy8I GTNNAC 2 cut(s) 487, 505
HpyAV CCTTC 2 cut(s) 43, 314
HpyCH4III ACNGT 2 cut(s) 188, 639
HpyCH4IV ACGT 2 cut(s) 395, 447
HpyCH4V TGCA 5 cut(s) 18, 329, 367, 380, 605
HpyF10VI GCNNNNNNNGC 1 cut(s) 389
HpyF3I CTNAG 1 cut(s) 384
HpySE526I ACGT 2 cut(s) 395, 447
Hsp92II CATG 4 cut(s) 230, 293, 377, 631
HspAI GCGC 2 cut(s) 275, 277
Kzo9I GATC 8 cut(s) 57, 66, 97, 178, 216, 369, 559, 648
LmnI GCTCC 2 cut(s) 51, 389
LpnPI CCDG 9 cut(s) 62, 199, 288, 336, 548, 555, 582, 590, 621
Lsp1109I GCAGC 2 cut(s) 392, 589
MaeI CTAG 1 cut(s) 258
MaeII ACGT 2 cut(s) 395, 447
MaeIII GTNAC 4 cut(s) 147, 182, 188, 355
MalI GATC 8 cut(s) 59, 68, 99, 180, 218, 371, 561, 650
MboI GATC 8 cut(s) 57, 66, 97, 178, 216, 369, 559, 648
MboII GAAGA 4 cut(s) 28, 146, 291, 526
MflI RGATCY 2 cut(s) 216, 369
MluCI AATT 4 cut(s) 13, 28, 314, 404
MnlI CCTC 2 cut(s) 182, 379
Mph1103I ATGCAT 1 cut(s) 331
MroXI GAANNNNTTC 1 cut(s) 32
MseI TTAA 3 cut(s) 11, 21, 407
MslI CAYNNNNRTG 2 cut(s) 23, 119
MspA1I CMGCKG 1 cut(s) 383
MspR9I CCNGG 1 cut(s) 570
MvaI CCWGG 1 cut(s) 570
MvnI CGCG 1 cut(s) 277
MwoI GCNNNNNNNGC 1 cut(s) 389
NcoI CCATGG 1 cut(s) 373
NdeII GATC 8 cut(s) 57, 66, 97, 178, 216, 369, 559, 648
NlaIII CATG 4 cut(s) 230, 293, 377, 631
NlaIV GGNNCC 3 cut(s) 263, 567, 575
NmeAIII GCCGAG 1 cut(s) 363
NmuCI GTSAC 1 cut(s) 188
NsiI ATGCAT 1 cut(s) 331
NspV TTCGAA 2 cut(s) 205, 318
OliI CACNNNNGTG 1 cut(s) 119
PaeR7I CTCGAG 1 cut(s) 616
PagI TCATGA 1 cut(s) 289
PauI GCGCGC 1 cut(s) 275
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 32
PfeI GAWTC 2 cut(s) 111, 632
PkrI GCNGC 2 cut(s) 382, 604
PpuMI RGGWCCY 1 cut(s) 574
Psp5II RGGWCCY 1 cut(s) 574
Psp6I CCWGG 1 cut(s) 568
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 3 cut(s) 263, 567, 575
PspPI GGNCC 3 cut(s) 261, 566, 574
PspPPI RGGWCCY 1 cut(s) 574
PspXI VCTCGAGB 1 cut(s) 616
PsuI RGATCY 2 cut(s) 216, 369
PteI GCGCGC 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 2 cut(s) 77, 346
RsaNI GTAC 2 cut(s) 76, 345
RseI CAYNNNNRTG 2 cut(s) 23, 119
SaqAI TTAA 3 cut(s) 11, 21, 407
SatI GCNGC 2 cut(s) 381, 603
Sau3AI GATC 8 cut(s) 57, 66, 97, 178, 216, 369, 559, 648
Sau96I GGNCC 3 cut(s) 261, 566, 574
ScrFI CCNGG 1 cut(s) 570
SetI ASST 7 cut(s) 48, 385, 394, 398, 450, 457, 579
Sfr274I CTCGAG 1 cut(s) 616
SfuI TTCGAA 2 cut(s) 205, 318
SinI GGWCC 2 cut(s) 566, 574
SlaI CTCGAG 1 cut(s) 616
SmiMI CAYNNNNRTG 2 cut(s) 23, 119
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
Sse9I AATT 4 cut(s) 13, 28, 314, 404
SsiI CCGC 1 cut(s) 79
SspMI CTAG 1 cut(s) 258
StyD4I CCNGG 1 cut(s) 568
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 2 cut(s) 188, 639
TaiI ACGT 2 cut(s) 398, 450
TaqI TCGA 5 cut(s) 174, 205, 318, 531, 617
TasI AATT 4 cut(s) 13, 28, 314, 404
TfiI GAWTC 2 cut(s) 111, 632
Tru1I TTAA 3 cut(s) 11, 21, 407
Tru9I TTAA 3 cut(s) 11, 21, 407
TscAI CASTG 2 cut(s) 100, 193
TseFI GTSAC 1 cut(s) 188
TseI GCWGC 2 cut(s) 380, 602
Tsp45I GTSAC 1 cut(s) 188
TspDTI ATGAA 2 cut(s) 41, 517
TspRI CASTG 2 cut(s) 100, 193
VpaK11BI GGWCC 2 cut(s) 566, 574
XapI RAATTY 1 cut(s) 28
XhoI CTCGAG 1 cut(s) 616
XmnI GAANNNNTTC 1 cut(s) 32
XspI CTAG 1 cut(s) 258
Zsp2I ATGCAT 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.