Rmu_sc0020284.1_g000003

Alginate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0020284.1
OG0000532
Physical Location & Seq
Forward (+)
14135 .. 14371
237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0020284.1_g000003.1.cds

Sequence Viewer

Length: 237 bp
atgttgagggtctacaatggttcactcacctactacaaggctccagtactggttacaaacatctacgataaatggttcaagatcaatgtggttcacgacgttgatgctgcgaaagtcagagtctacattgacggctgtctcaaaatcgaggcaaatggtcgtggaggaacgtctcatgctttcaaatttggagtgcatgtgaaaacaccagatggaataataactctacttgaataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

78

Amino Acids

8.74

Weight (kDa)

9.13

Isoelectric Point (pI)

12.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 12, 123
AcsI RAATTY 1 cut(s) 185
AfaI GTAC 1 cut(s) 48
AgsI TTSAA 3 cut(s) 79, 184, 233
Alw26I GTCTC 2 cut(s) 143, 177
ApeKI GCWGC 1 cut(s) 107
ApoI RAATTY 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 19
BbvI GCAGC 1 cut(s) 94
BccI CCATC 1 cut(s) 206
BceAI ACGGC 1 cut(s) 148
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BcoDI GTCTC 2 cut(s) 143, 177
BisI GCNGC 1 cut(s) 108
BlsI GCNGC 1 cut(s) 109
BmcAI AGTACT 1 cut(s) 48
BmiI GGNNCC 1 cut(s) 42
BmsI GCATC 1 cut(s) 94
BoxI GACNNNNGTC 1 cut(s) 135
BpmI CTGGAG 1 cut(s) 27
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bse1I ACTGG 2 cut(s) 44, 54
BseNI ACTGG 2 cut(s) 44, 54
BseXI GCAGC 1 cut(s) 94
BsmAI GTCTC 2 cut(s) 143, 177
BsmBI CGTCTC 1 cut(s) 177
Bsp143I GATC 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 42
BsrI ACTGG 2 cut(s) 44, 54
BssMI GATC 1 cut(s) 81
BstKTI GATC 1 cut(s) 84
BstMAI GTCTC 2 cut(s) 143, 177
BstMBI GATC 1 cut(s) 81
BstNSI RCATGY 1 cut(s) 200
BstPAI GACNNNNGTC 1 cut(s) 135
BstV1I GCAGC 1 cut(s) 94
Csp6I GTAC 1 cut(s) 47
CviAII CATG 2 cut(s) 176, 197
CviJI RGCY 2 cut(s) 41, 135
CviKI_1 RGCY 2 cut(s) 41, 135
CviQI GTAC 1 cut(s) 47
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 2 cut(s) 179, 200
FaiI YATR 2 cut(s) 177, 198
FatI CATG 2 cut(s) 175, 196
FblI GTMKAC 2 cut(s) 12, 123
Fnu4HI GCNGC 1 cut(s) 108
Fsp4HI GCNGC 1 cut(s) 108
GluI GCNGC 1 cut(s) 108
GsuI CTGGAG 1 cut(s) 27
Hin1II CATG 2 cut(s) 179, 200
HinfI GANTC 1 cut(s) 120
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 4 cut(s) 13, 23, 94, 124
Hpy188I TCNGA 1 cut(s) 119
Hpy188III TCNNGA 2 cut(s) 79, 95
Hpy8I GTNNAC 4 cut(s) 13, 23, 94, 124
Hpy99I CGWCG 1 cut(s) 101
HpyCH4IV ACGT 2 cut(s) 99, 170
HpyCH4V TGCA 1 cut(s) 196
HpySE526I ACGT 2 cut(s) 99, 170
Hsp92II CATG 2 cut(s) 179, 200
Kzo9I GATC 1 cut(s) 81
LmnI GCTCC 1 cut(s) 46
LpnPI CCDG 3 cut(s) 35, 57, 222
Lsp1109I GCAGC 1 cut(s) 94
LweI GCATC 1 cut(s) 94
MaeII ACGT 2 cut(s) 99, 170
MaeIII GTNAC 1 cut(s) 52
MalI GATC 1 cut(s) 83
MboI GATC 1 cut(s) 81
MluCI AATT 1 cut(s) 185
MlyI GAGTC 1 cut(s) 129
MnlI CCTC 2 cut(s) 142, 158
NdeII GATC 1 cut(s) 81
NlaIII CATG 2 cut(s) 179, 200
NlaIV GGNNCC 1 cut(s) 42
NspI RCATGY 1 cut(s) 200
PkrI GCNGC 1 cut(s) 109
PleI GAGTC 1 cut(s) 128
PpsI GAGTC 1 cut(s) 128
PshAI GACNNNNGTC 1 cut(s) 135
PspN4I GGNNCC 1 cut(s) 42
RsaI GTAC 1 cut(s) 48
RsaNI GTAC 1 cut(s) 47
SatI GCNGC 1 cut(s) 108
Sau3AI GATC 1 cut(s) 81
ScaI AGTACT 1 cut(s) 48
SchI GAGTC 1 cut(s) 129
SetI ASST 3 cut(s) 32, 102, 173
SfaNI GCATC 1 cut(s) 94
Sse9I AATT 1 cut(s) 185
TaiI ACGT 2 cut(s) 102, 173
TaqI TCGA 1 cut(s) 147
TasI AATT 1 cut(s) 185
TatI WGTACW 1 cut(s) 46
TseI GCWGC 1 cut(s) 107
XapI RAATTY 1 cut(s) 185
XceI RCATGY 1 cut(s) 200
XmiI GTMKAC 2 cut(s) 12, 123
ZrmI AGTACT 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.