Rorug02G0223500

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000532
Physical Location & Seq
Forward (+)
21662641 .. 21664711
2071 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0223500.1

Sequence Viewer

Length: 447 bp
ATGCTCGAAGCACATTTTTTGTCAAGTGGTTTTGTTGTGATGCTTTTTCATTATGATGGTAACGTTGAGGAGTGGATGCAATTTCAATGGAATGATCTTGTCATTTATGTGTCTGCAGTCAATCAAACAAAATGGTGGTTCGCAAAGCGTTTCTTACATCGTGATATAGTTGATCAATATAGTCGCATTCTTCTACGGGATGAGGACCTTGGAGTTGACAATTTCAATCCTCAACGGTATGTGTCCATTGTTCGAAAGGAAGGGCTTGAAATATCACAACCGGCACTTGATTTTTTCAAGACAGAGGTGAACTACATGGAGAAACTTTCAAAAGATGAGGGGATTCAGAAGAAACTCAGCAAGGATATTTTGCAATATAAAGCTCATATAATCAAGATATTTGTGGAACATAGGCACAATTGGATCTCTGCAGATTTAGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

148

Amino Acids

17.82

Weight (kDa)

5.85

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF707 PF05212 5 - 115 2.8e-47 Protein of unknown function (DUF707)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 63
AclWI GGATC 1 cut(s) 431
AgsI TTSAA 5 cut(s) 86, 226, 269, 298, 330
AluBI AGCT 1 cut(s) 383
AluI AGCT 1 cut(s) 383
AlwI GGATC 1 cut(s) 431
AspS9I GGNCC 1 cut(s) 205
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 253
AvaII GGWCC 1 cut(s) 205
BccI CCATC 1 cut(s) 50
BclI TGATCA 1 cut(s) 172
BfmI CTRYAG 2 cut(s) 114, 429
Bme18I GGWCC 1 cut(s) 205
BmgT120I GGNCC 1 cut(s) 205
BmsI GCATC 2 cut(s) 30, 66
Bpu14I TTCGAA 1 cut(s) 253
BsaJI CCNNGG 1 cut(s) 208
Bse118I RCCGGY 1 cut(s) 280
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 2 cut(s) 81, 205
BseMII CTCAG 1 cut(s) 370
BseRI GAGGAG 1 cut(s) 83
BsiSI CCGG 1 cut(s) 281
BsmI GAATGC 1 cut(s) 186
Bsp119I TTCGAA 1 cut(s) 253
Bsp143I GATC 3 cut(s) 94, 172, 423
BspCNI CTCAG 1 cut(s) 369
BspMAI CTGCAG 2 cut(s) 118, 433
BspPI GGATC 1 cut(s) 431
BspT104I TTCGAA 1 cut(s) 253
BsrFI RCCGGY 1 cut(s) 280
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 3 cut(s) 94, 172, 423
BssT1I CCWWGG 1 cut(s) 208
Bst4CI ACNGT 1 cut(s) 237
BstBI TTCGAA 1 cut(s) 253
BstDEI CTNAG 1 cut(s) 356
BstF5I GGATG 2 cut(s) 81, 205
BstKTI GATC 3 cut(s) 97, 175, 426
BstMBI GATC 3 cut(s) 94, 172, 423
BstSFI CTRYAG 2 cut(s) 114, 429
BstX2I RGATCY 1 cut(s) 423
BstYI RGATCY 1 cut(s) 423
BtsCI GGATG 2 cut(s) 81, 205
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 1 cut(s) 205
CviAII CATG 1 cut(s) 316
CviJI RGCY 2 cut(s) 265, 383
CviKI_1 RGCY 2 cut(s) 265, 383
DdeI CTNAG 1 cut(s) 356
DpnI GATC 3 cut(s) 96, 174, 425
DpnII GATC 3 cut(s) 94, 172, 423
Eco130I CCWWGG 1 cut(s) 208
Eco47I GGWCC 1 cut(s) 205
EcoO109I RGGNCCY 1 cut(s) 205
EcoT14I CCWWGG 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 208
FaeI CATG 1 cut(s) 319
FalI AAGNNNNNCTT 2 cut(s) 137, 169
FatI CATG 1 cut(s) 315
FbaI TGATCA 1 cut(s) 172
FokI GGATG 2 cut(s) 88, 212
HapII CCGG 1 cut(s) 281
Hin1II CATG 1 cut(s) 319
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 1 cut(s) 343
HpaII CCGG 1 cut(s) 281
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 217, 310
Hpy188I TCNGA 1 cut(s) 348
Hpy188III TCNNGA 3 cut(s) 161, 298, 394
Hpy8I GTNNAC 2 cut(s) 217, 310
HpyAV CCTTC 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 237
HpyCH4IV ACGT 1 cut(s) 63
HpyCH4V TGCA 4 cut(s) 79, 116, 373, 431
HpyF3I CTNAG 1 cut(s) 356
HpySE526I ACGT 1 cut(s) 63
Hsp92II CATG 1 cut(s) 319
Ksp22I TGATCA 1 cut(s) 172
Kzo9I GATC 3 cut(s) 94, 172, 423
LpnPI CCDG 1 cut(s) 294
LweI GCATC 2 cut(s) 30, 66
MaeII ACGT 1 cut(s) 63
MaeIII GTNAC 1 cut(s) 59
MalI GATC 3 cut(s) 96, 174, 425
MboI GATC 3 cut(s) 94, 172, 423
MboII GAAGA 2 cut(s) 182, 361
MfeI CAATTG 1 cut(s) 418
MflI RGATCY 1 cut(s) 423
MluCI AATT 3 cut(s) 80, 220, 418
MnlI CCTC 5 cut(s) 61, 196, 240, 298, 331
MslI CAYNNNNRTG 2 cut(s) 54, 107
MspI CCGG 1 cut(s) 281
MunI CAATTG 1 cut(s) 418
Mva1269I GAATGC 1 cut(s) 186
NdeII GATC 3 cut(s) 94, 172, 423
NlaIII CATG 1 cut(s) 319
NspV TTCGAA 1 cut(s) 253
PctI GAATGC 1 cut(s) 186
PfeI GAWTC 1 cut(s) 343
PpuMI RGGWCCY 1 cut(s) 205
Psp1406I AACGTT 1 cut(s) 63
Psp5II RGGWCCY 1 cut(s) 205
PspPI GGNCC 1 cut(s) 205
PspPPI RGGWCCY 1 cut(s) 205
PstI CTGCAG 2 cut(s) 118, 433
PsuI RGATCY 1 cut(s) 423
RseI CAYNNNNRTG 2 cut(s) 54, 107
Sau3AI GATC 3 cut(s) 94, 172, 423
Sau96I GGNCC 1 cut(s) 205
SetI ASST 4 cut(s) 66, 210, 309, 385
SfaNI GCATC 2 cut(s) 30, 66
SfcI CTRYAG 2 cut(s) 114, 429
SfuI TTCGAA 1 cut(s) 253
SinI GGWCC 1 cut(s) 205
SmiMI CAYNNNNRTG 2 cut(s) 54, 107
Sse9I AATT 3 cut(s) 80, 220, 418
StyI CCWWGG 1 cut(s) 208
TaaI ACNGT 1 cut(s) 237
TaiI ACGT 1 cut(s) 66
TaqI TCGA 2 cut(s) 6, 253
TasI AATT 3 cut(s) 80, 220, 418
TfiI GAWTC 1 cut(s) 343
TspDTI ATGAA 1 cut(s) 38
VpaK11BI GGWCC 1 cut(s) 205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.