MD15G1341000.v1.1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000532
Physical Location & Seq
Reverse (-)
38604756 .. 38607925
3170 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1341000.v1.1.491

Sequence Viewer

Length: 660 bp
ATGGGTATCCTTAATCACATTGCTTTGATAAGCGCAGCTTTTGTTATCCTACAAACTCGTTGGTCAAGTAGTTGGCCTATGGATCCAACCCTTGGCTTCCTACGACTCCCACTAAACAAATCCAACTTTCACGTCCAAAAACCATATGATTTGCAAGAAAGTGAGCGCTACAGCTTTAAACATGGAGTGCACAAGATGTGGGTATACTTCACGGACAAGCCCCTCTATCGTAATAGTCCTACCCATCCTCGAACTGAGATCCGAATAACGGGATACGATTATTCTTCGGGTGTATGGCAATTCGAAGGATACGGGTATGTACCATGTGGAACAGCCAATGTCACCATAATGCAAGTGTTTGGGGGAAGTCCTCATGCAACAACGTTTATGCTTAGAGTTGGCCAAAATGGTACCCTTACATATTATAGGAGTGCTGTTTTGGCTTCAAACATGTACGACAGGTGGTTCAGGCTTAATGTGATCCATGATGCCAATGCTTCGCATTTAAAGGTTTATATTGATGGAGTTCTCAAGTTTGAGGCACCTGGTCGGGGAGGGACCTCGCATTATTTCAAATGTGGTGTCTACTCGCAGCCAGATGGTTCCTACTACATGGAGTCTCGTTGGAAGGGAATCAAAGTACTCAAGAAATGTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

220

Amino Acids

25.09

Weight (kDa)

9.5

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 41 - 214 9.8e-28 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 410, 541
AccB7I CCANNNNNTGG 1 cut(s) 92
AccI GTMKAC 2 cut(s) 204, 585
AclI AACGTT 1 cut(s) 383
AclWI GGATC 4 cut(s) 77, 90, 253, 475
AcoI YGGCCR 1 cut(s) 400
AfaI GTAC 4 cut(s) 321, 412, 455, 642
AfeI AGCGCT 1 cut(s) 167
AfiI CCNNNNNNNGG 3 cut(s) 92, 268, 551
AflIII ACRYGT 1 cut(s) 450
AgsI TTSAA 2 cut(s) 447, 574
AjiI CACGTC 1 cut(s) 133
AjnI CCWGG 1 cut(s) 544
AluBI AGCT 2 cut(s) 38, 174
AluI AGCT 2 cut(s) 38, 174
Alw21I GWGCWC 1 cut(s) 192
Alw26I GTCTC 1 cut(s) 624
Alw44I GTGCAC 1 cut(s) 188
AlwI GGATC 4 cut(s) 77, 90, 253, 475
Aor51HI AGCGCT 1 cut(s) 167
AoxI GGCC 2 cut(s) 74, 400
ApaLI GTGCAC 1 cut(s) 188
ApeKI GCWGC 2 cut(s) 35, 592
ArsI GACNNNNNNTTYG 2 cut(s) 567, 599
Asp718I GGTACC 1 cut(s) 410
AspLEI GCGC 2 cut(s) 35, 168
AspS9I GGNCC 1 cut(s) 558
AsuHPI GGTGA 1 cut(s) 334
AsuII TTCGAA 1 cut(s) 303
AvaII GGWCC 1 cut(s) 558
BaeGI GKGCMC 1 cut(s) 192
BalI TGGCCA 1 cut(s) 402
BamHI GGATCC 1 cut(s) 82
BanI GGYRCC 2 cut(s) 410, 541
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 47, 604
BccI CCATC 3 cut(s) 252, 515, 593
BciT130I CCWGG 1 cut(s) 546
BciVI GTATCC 3 cut(s) 17, 266, 302
BcoDI GTCTC 1 cut(s) 624
BfmI CTRYAG 1 cut(s) 169
BfoI RGCGCY 1 cut(s) 169
BfuI GTATCC 3 cut(s) 17, 266, 302
BisI GCNGC 2 cut(s) 36, 593
BlsI GCNGC 2 cut(s) 37, 594
BmcAI AGTACT 1 cut(s) 642
Bme1390I CCNGG 1 cut(s) 546
Bme18I GGWCC 1 cut(s) 558
BmgBI CACGTC 1 cut(s) 133
BmgT120I GGNCC 1 cut(s) 558
BmiI GGNNCC 5 cut(s) 84, 412, 543, 559, 604
BmrFI CCNGG 1 cut(s) 546
BmsI GCATC 1 cut(s) 478
Bpu14I TTCGAA 1 cut(s) 303
BpuEI CTTGAG 2 cut(s) 515, 629
BsaJI CCNNGG 1 cut(s) 91
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 268, 551
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 546
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 3 cut(s) 92, 268, 551
BseMI GCAATG 1 cut(s) 18
BseMII CTCAG 1 cut(s) 246
BseSI GKGCMC 1 cut(s) 192
BseXI GCAGC 2 cut(s) 47, 604
BshFI GGCC 2 cut(s) 76, 402
BshNI GGYRCC 2 cut(s) 410, 541
BsiHKAI GWGCWC 1 cut(s) 192
BslFI GGGAC 1 cut(s) 571
BslI CCNNNNNNNGG 3 cut(s) 92, 268, 551
BsmAI GTCTC 1 cut(s) 624
BsmFI GGGAC 1 cut(s) 571
BsnI GGCC 2 cut(s) 76, 402
Bsp119I TTCGAA 1 cut(s) 303
Bsp1286I GDGCHC 1 cut(s) 192
Bsp143I GATC 3 cut(s) 82, 258, 480
BspANI GGCC 2 cut(s) 76, 402
BspCNI CTCAG 1 cut(s) 247
BspLI GGNNCC 5 cut(s) 84, 412, 543, 559, 604
BspPI GGATC 4 cut(s) 77, 90, 253, 475
BspT104I TTCGAA 1 cut(s) 303
BspT107I GGYRCC 2 cut(s) 410, 541
BsrDI GCAATG 1 cut(s) 18
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 3 cut(s) 82, 258, 480
BssNAI GTATAC 1 cut(s) 205
BssT1I CCWWGG 1 cut(s) 91
Bst1107I GTATAC 1 cut(s) 205
Bst2UI CCWGG 1 cut(s) 546
BstBI TTCGAA 1 cut(s) 303
BstDEI CTNAG 2 cut(s) 255, 392
BstF5I GGATG 1 cut(s) 244
BstH2I RGCGCY 1 cut(s) 169
BstHHI GCGC 2 cut(s) 35, 168
BstKTI GATC 3 cut(s) 85, 261, 483
BstMAI GTCTC 1 cut(s) 624
BstMBI GATC 3 cut(s) 82, 258, 480
BstMWI GCNNNNNNNGC 1 cut(s) 440
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 1 cut(s) 454
BstSCI CCNGG 1 cut(s) 544
BstSFI CTRYAG 1 cut(s) 169
BstSLI GKGCMC 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 47, 604
BstX2I RGATCY 2 cut(s) 82, 258
BstYI RGATCY 2 cut(s) 82, 258
BstZ17I GTATAC 1 cut(s) 205
BsuI GTATCC 3 cut(s) 17, 266, 302
BsuRI GGCC 2 cut(s) 76, 402
BtrI CACGTC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 244
CfoI GCGC 2 cut(s) 35, 168
Cfr13I GGNCC 1 cut(s) 558
CsiI ACCWGGT 1 cut(s) 544
Csp6I GTAC 4 cut(s) 320, 411, 454, 641
CspCI CAANNNNNGTGG 1 cut(s) 634
CviAII CATG 6 cut(s) 182, 324, 374, 451, 485, 613
CviQI GTAC 4 cut(s) 320, 411, 454, 641
DdeI CTNAG 2 cut(s) 255, 392
DpnI GATC 3 cut(s) 84, 260, 482
DpnII GATC 3 cut(s) 82, 258, 480
DraI TTTAAA 2 cut(s) 178, 507
EaeI YGGCCR 1 cut(s) 400
Eco130I CCWWGG 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 558
Eco47III AGCGCT 1 cut(s) 167
EcoO109I RGGNCCY 1 cut(s) 558
EcoRII CCWGG 1 cut(s) 544
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 6 cut(s) 185, 327, 377, 454, 488, 616
FalI AAGNNNNNCTT 2 cut(s) 22, 54
FaqI GGGAC 1 cut(s) 571
FatI CATG 6 cut(s) 181, 323, 373, 450, 484, 612
FauNDI CATATG 1 cut(s) 145
FblI GTMKAC 2 cut(s) 204, 585
Fnu4HI GCNGC 2 cut(s) 36, 593
FokI GGATG 1 cut(s) 231
Fsp4HI GCNGC 2 cut(s) 36, 593
GlaI GCGC 2 cut(s) 34, 167
GluI GCNGC 2 cut(s) 36, 593
HaeII RGCGCY 1 cut(s) 169
HaeIII GGCC 2 cut(s) 76, 402
HhaI GCGC 2 cut(s) 35, 168
Hin1II CATG 6 cut(s) 185, 327, 377, 454, 488, 616
Hin6I GCGC 2 cut(s) 33, 166
HinP1I GCGC 2 cut(s) 33, 166
HinfI GANTC 3 cut(s) 105, 617, 633
HphI GGTGA 1 cut(s) 334
Hpy166II GTNNAC 3 cut(s) 190, 205, 586
Hpy188I TCNGA 1 cut(s) 263
Hpy188III TCNNGA 1 cut(s) 646
Hpy8I GTNNAC 3 cut(s) 190, 205, 586
HpyAV CCTTC 2 cut(s) 299, 622
HpyCH4IV ACGT 2 cut(s) 132, 383
HpyCH4V TGCA 4 cut(s) 154, 190, 352, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 440
HpyF3I CTNAG 2 cut(s) 255, 392
HpySE526I ACGT 2 cut(s) 132, 383
Hsp92II CATG 6 cut(s) 185, 327, 377, 454, 488, 616
HspAI GCGC 2 cut(s) 33, 166
KpnI GGTACC 1 cut(s) 414
Kzo9I GATC 3 cut(s) 82, 258, 480
LpnPI CCDG 5 cut(s) 445, 454, 531, 558, 609
Lsp1109I GCAGC 2 cut(s) 47, 604
LweI GCATC 1 cut(s) 478
MabI ACCWGGT 1 cut(s) 544
MaeII ACGT 2 cut(s) 132, 383
MaeIII GTNAC 1 cut(s) 340
MalI GATC 3 cut(s) 84, 260, 482
MboI GATC 3 cut(s) 82, 258, 480
MboII GAAGA 1 cut(s) 276
MflI RGATCY 2 cut(s) 82, 258
MhlI GDGCHC 1 cut(s) 192
MlsI TGGCCA 1 cut(s) 402
MluCI AATT 1 cut(s) 299
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 2 cut(s) 99, 626
MmeI TCCRAC 3 cut(s) 110, 147, 605
MnlI CCTC 6 cut(s) 233, 258, 381, 532, 548, 571
Mox20I TGGCCA 1 cut(s) 402
MscI TGGCCA 1 cut(s) 402
MseI TTAA 4 cut(s) 12, 177, 474, 506
MslI CAYNNNNRTG 1 cut(s) 347
Msp20I TGGCCA 1 cut(s) 402
MspR9I CCNGG 1 cut(s) 546
MvaI CCWGG 1 cut(s) 546
MwoI GCNNNNNNNGC 1 cut(s) 440
NdeI CATATG 1 cut(s) 145
NdeII GATC 3 cut(s) 82, 258, 480
NlaIII CATG 6 cut(s) 185, 327, 377, 454, 488, 616
NlaIV GGNNCC 5 cut(s) 84, 412, 543, 559, 604
NmuCI GTSAC 1 cut(s) 340
NspI RCATGY 1 cut(s) 454
NspV TTCGAA 1 cut(s) 303
PciI ACATGT 1 cut(s) 450
PfeI GAWTC 1 cut(s) 633
PflMI CCANNNNNTGG 1 cut(s) 92
PkrI GCNGC 2 cut(s) 37, 594
PleI GAGTC 2 cut(s) 99, 625
PpsI GAGTC 2 cut(s) 99, 625
PpuMI RGGWCCY 1 cut(s) 558
PscI ACATGT 1 cut(s) 450
Psp1406I AACGTT 1 cut(s) 383
Psp5II RGGWCCY 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 544
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 5 cut(s) 84, 412, 543, 559, 604
PspPI GGNCC 1 cut(s) 558
PspPPI RGGWCCY 1 cut(s) 558
PsuI RGATCY 2 cut(s) 82, 258
RsaI GTAC 4 cut(s) 321, 412, 455, 642
RsaNI GTAC 4 cut(s) 320, 411, 454, 641
RseI CAYNNNNRTG 1 cut(s) 347
SaqAI TTAA 4 cut(s) 12, 177, 474, 506
SatI GCNGC 2 cut(s) 36, 593
Sau3AI GATC 3 cut(s) 82, 258, 480
Sau96I GGNCC 1 cut(s) 558
ScaI AGTACT 1 cut(s) 642
SchI GAGTC 2 cut(s) 99, 626
ScrFI CCNGG 1 cut(s) 546
SduI GDGCHC 1 cut(s) 192
SetI ASST 8 cut(s) 40, 135, 176, 386, 464, 513, 547, 563
SexAI ACCWGGT 1 cut(s) 544
SfaNI GCATC 1 cut(s) 478
SfcI CTRYAG 1 cut(s) 169
SfuI TTCGAA 1 cut(s) 303
SinI GGWCC 1 cut(s) 558
SmiMI CAYNNNNRTG 1 cut(s) 347
SmlI CTYRAG 2 cut(s) 530, 644
SmoI CTYRAG 2 cut(s) 530, 644
Sse9I AATT 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 544
StyI CCWWGG 1 cut(s) 91
TaiI ACGT 2 cut(s) 135, 386
TaqI TCGA 2 cut(s) 250, 303
TasI AATT 1 cut(s) 299
TatI WGTACW 1 cut(s) 640
TfiI GAWTC 1 cut(s) 633
Tru1I TTAA 4 cut(s) 12, 177, 474, 506
Tru9I TTAA 4 cut(s) 12, 177, 474, 506
TseFI GTSAC 1 cut(s) 340
TseI GCWGC 2 cut(s) 35, 592
Tsp45I GTSAC 1 cut(s) 340
TspGWI ACGGA 1 cut(s) 227
Van91I CCANNNNNTGG 1 cut(s) 92
VneI GTGCAC 1 cut(s) 188
VpaK11BI GGWCC 1 cut(s) 558
XceI RCATGY 1 cut(s) 454
XmiI GTMKAC 2 cut(s) 204, 585
ZrmI AGTACT 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.