FvH4_1g23860

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000532
Physical Location & Seq
Forward (+)
15718904 .. 15720774
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23860.t1

Sequence Viewer

Length: 669 bp
ATGGGTGCCAATATAAACCACATTGTTTTGGCACGCACAGCGTTTTTTCTTGTCCTAATAACACAATGGTCGAGCAGCTGGCCTGTGGATAATCCAACTCTTGGCTTCCTAGAACTCCCATTAAACACATCCAATTTGGACATCCAGAGACCATATGACCTGCCTCTAAACGAGCGCTATAGCTTCATAGATGGAGTGCATAAGCTATGGGTATACTTCAGCGACAAGCCCCACTCGCCGGAAAGCCAGACTCATGCTCGAACCGAAATCCGAATAAGGGGATATGATTATTCTTCATGTGTATGGCAATTCGAAGGATACGGGTATGTACCATGTGGAACAGCCAATGTGACCATAATGCAAGTGTTTGGGGCAAGCCCTCACGCAACCACTCTAATGCTTAGAGTTGGTTCAAATGGTACACTTACATACTACAGGACTGCAGTCCTGGATCCTTACATCTACGACAGATGGTTCCGACTTAACGTGATCCACGACGTCTACGCCTCGCATTTGAAGGTTTATATCGATGGAGTCCTCAAGTTTGAGGCACCCGGTCGAGGAGGAAAGTTTCATTATTTCAAATGCGGAGTCTACGCTCAGCCGGATGGTTCCTACTACATGGAGTCTCGTTGGAAAGGGATTAAGGTGCTGAAAAAATGTGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

223

Amino Acids

25.53

Weight (kDa)

8.57

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 42 - 217 4.3e-26 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 501
Acc36I ACCTGC 1 cut(s) 168
AccB1I GGYRCC 2 cut(s) 5, 550
AccB7I CCANNNNNTGG 1 cut(s) 101
AccI GTMKAC 3 cut(s) 213, 501, 594
AciI CCGC 1 cut(s) 588
AclWI GGATC 3 cut(s) 446, 459, 484
AcuI CTGAAG 1 cut(s) 202
AcyI GRCGYC 1 cut(s) 498
AfaI GTAC 2 cut(s) 330, 421
AfeI AGCGCT 1 cut(s) 176
AfiI CCNNNNNNNGG 4 cut(s) 101, 238, 277, 560
AgsI TTSAA 3 cut(s) 414, 517, 583
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 3 cut(s) 78, 183, 205
AluI AGCT 3 cut(s) 78, 183, 205
Alw26I GTCTC 2 cut(s) 142, 633
AlwI GGATC 3 cut(s) 446, 459, 484
Aor51HI AGCGCT 1 cut(s) 176
AoxI GGCC 1 cut(s) 80
ApeKI GCWGC 1 cut(s) 75
ArsI GACNNNNNNTTYG 2 cut(s) 576, 608
AspLEI GCGC 1 cut(s) 177
AsuC2I CCSGG 1 cut(s) 555
AsuII TTCGAA 1 cut(s) 312
BamHI GGATCC 1 cut(s) 451
BanI GGYRCC 2 cut(s) 5, 550
BbvI GCAGC 1 cut(s) 87
BccI CCATC 4 cut(s) 185, 465, 524, 602
BciT130I CCWGG 1 cut(s) 449
BciVI GTATCC 1 cut(s) 311
BcnI CCSGG 1 cut(s) 555
BcoDI GTCTC 2 cut(s) 142, 633
BfaI CTAG 2 cut(s) 110, 667
BfmI CTRYAG 3 cut(s) 178, 433, 441
BfoI RGCGCY 1 cut(s) 178
BfuAI ACCTGC 1 cut(s) 168
BfuI GTATCC 1 cut(s) 311
BisI GCNGC 1 cut(s) 76
BlpI GCTNAGC 1 cut(s) 600
BlsI GCNGC 1 cut(s) 77
Bme1390I CCNGG 2 cut(s) 449, 555
BmiI GGNNCC 5 cut(s) 7, 453, 476, 552, 613
BmrFI CCNGG 2 cut(s) 449, 555
BoxI GACNNNNGTC 1 cut(s) 443
Bpu1102I GCTNAGC 1 cut(s) 600
Bpu14I TTCGAA 1 cut(s) 312
BpuEI CTTGAG 1 cut(s) 524
BpuMI CCSGG 1 cut(s) 555
Bsa29I ATCGAT 1 cut(s) 528
BsaHI GRCGYC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 142
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 238, 277, 560
BseBI CCWGG 1 cut(s) 449
BseCI ATCGAT 1 cut(s) 528
BseGI GGATG 3 cut(s) 128, 141, 613
BseLI CCNNNNNNNGG 4 cut(s) 101, 238, 277, 560
BseMII CTCAG 1 cut(s) 614
BseRI GAGGAG 1 cut(s) 576
BseXI GCAGC 1 cut(s) 87
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 1 cut(s) 82
BshNI GGYRCC 2 cut(s) 5, 550
BshVI ATCGAT 1 cut(s) 528
BsiEI CGRYCG 1 cut(s) 559
BsiSI CCGG 3 cut(s) 239, 555, 605
BslI CCNNNNNNNGG 4 cut(s) 101, 238, 277, 560
BsmAI GTCTC 2 cut(s) 142, 633
BsnI GGCC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 142
Bsp119I TTCGAA 1 cut(s) 312
Bsp143I GATC 2 cut(s) 451, 489
Bsp1720I GCTNAGC 1 cut(s) 600
BspACI CCGC 1 cut(s) 588
BspANI GGCC 1 cut(s) 82
BspCNI CTCAG 1 cut(s) 613
BspDI ATCGAT 1 cut(s) 528
BspLI GGNNCC 5 cut(s) 7, 453, 476, 552, 613
BspMAI CTGCAG 1 cut(s) 445
BspMI ACCTGC 1 cut(s) 168
BspPI GGATC 3 cut(s) 446, 459, 484
BspT104I TTCGAA 1 cut(s) 312
BspT107I GGYRCC 2 cut(s) 5, 550
BspTNI GGTCTC 1 cut(s) 142
BssMI GATC 2 cut(s) 451, 489
BssNAI GTATAC 1 cut(s) 214
BssNI GRCGYC 1 cut(s) 498
Bst1107I GTATAC 1 cut(s) 214
Bst2UI CCWGG 1 cut(s) 449
BstACI GRCGYC 1 cut(s) 498
BstBI TTCGAA 1 cut(s) 312
BstC8I GCNNGC 3 cut(s) 34, 80, 376
BstDEI CTNAG 2 cut(s) 401, 600
BstF5I GGATG 3 cut(s) 128, 141, 613
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 1 cut(s) 177
BstKTI GATC 2 cut(s) 454, 492
BstMAI GTCTC 2 cut(s) 142, 633
BstMBI GATC 2 cut(s) 451, 489
BstMCI CGRYCG 1 cut(s) 559
BstMWI GCNNNNNNNGC 2 cut(s) 38, 235
BstNI CCWGG 1 cut(s) 449
BstPAI GACNNNNGTC 1 cut(s) 443
BstSCI CCNGG 2 cut(s) 447, 553
BstSFI CTRYAG 3 cut(s) 178, 433, 441
BstV1I GCAGC 1 cut(s) 87
BstX2I RGATCY 1 cut(s) 451
BstYI RGATCY 1 cut(s) 451
BstZ17I GTATAC 1 cut(s) 214
Bsu15I ATCGAT 1 cut(s) 528
BsuI GTATCC 1 cut(s) 311
BsuRI GGCC 1 cut(s) 82
BsuTUI ATCGAT 1 cut(s) 528
BtsCI GGATG 3 cut(s) 128, 141, 613
BveI ACCTGC 1 cut(s) 168
Cac8I GCNNGC 3 cut(s) 34, 80, 376
CfoI GCGC 1 cut(s) 177
ClaI ATCGAT 1 cut(s) 528
Csp6I GTAC 2 cut(s) 329, 420
CviAII CATG 4 cut(s) 254, 297, 333, 622
CviQI GTAC 2 cut(s) 329, 420
DdeI CTNAG 2 cut(s) 401, 600
DpnI GATC 2 cut(s) 453, 491
DpnII GATC 2 cut(s) 451, 489
Eco31I GGTCTC 1 cut(s) 142
Eco47III AGCGCT 1 cut(s) 176
Eco57I CTGAAG 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 447
FaeI CATG 4 cut(s) 257, 300, 336, 625
FatI CATG 4 cut(s) 253, 296, 332, 621
FauNDI CATATG 1 cut(s) 154
FblI GTMKAC 3 cut(s) 213, 501, 594
Fnu4HI GCNGC 1 cut(s) 76
FokI GGATG 3 cut(s) 115, 128, 620
Fsp4HI GCNGC 1 cut(s) 76
FspBI CTAG 2 cut(s) 110, 667
GlaI GCGC 1 cut(s) 176
GluI GCNGC 1 cut(s) 76
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 1 cut(s) 82
HapII CCGG 3 cut(s) 239, 555, 605
HhaI GCGC 1 cut(s) 177
Hin1I GRCGYC 1 cut(s) 498
Hin1II CATG 4 cut(s) 257, 300, 336, 625
Hin6I GCGC 1 cut(s) 175
HinP1I GCGC 1 cut(s) 175
HinfI GANTC 4 cut(s) 250, 534, 591, 626
HpaII CCGG 3 cut(s) 239, 555, 605
Hpy166II GTNNAC 4 cut(s) 214, 422, 502, 595
Hpy188I TCNGA 2 cut(s) 272, 479
Hpy188III TCNNGA 1 cut(s) 145
Hpy8I GTNNAC 4 cut(s) 214, 422, 502, 595
Hpy99I CGWCG 1 cut(s) 500
HpyAV CCTTC 2 cut(s) 308, 511
HpyCH4IV ACGT 2 cut(s) 486, 498
HpyCH4V TGCA 3 cut(s) 199, 361, 443
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 235
HpyF3I CTNAG 2 cut(s) 401, 600
HpySE526I ACGT 2 cut(s) 486, 498
Hsp92I GRCGYC 1 cut(s) 498
Hsp92II CATG 4 cut(s) 257, 300, 336, 625
HspAI GCGC 1 cut(s) 175
Kzo9I GATC 2 cut(s) 451, 489
Lsp1109I GCAGC 1 cut(s) 87
MaeI CTAG 2 cut(s) 110, 667
MaeII ACGT 2 cut(s) 486, 498
MaeIII GTNAC 2 cut(s) 349, 662
MalI GATC 2 cut(s) 453, 491
MboI GATC 2 cut(s) 451, 489
MboII GAAGA 1 cut(s) 285
MflI RGATCY 1 cut(s) 451
MluCI AATT 2 cut(s) 133, 308
MlyI GAGTC 4 cut(s) 244, 543, 600, 635
MmeI TCCRAC 3 cut(s) 119, 502, 614
MnlI CCTC 7 cut(s) 174, 390, 517, 541, 548, 554, 557
MseI TTAA 3 cut(s) 122, 483, 645
MslI CAYNNNNRTG 2 cut(s) 301, 395
MspA1I CMGCKG 1 cut(s) 78
MspI CCGG 3 cut(s) 239, 555, 605
MspR9I CCNGG 2 cut(s) 449, 555
MvaI CCWGG 1 cut(s) 449
MwoI GCNNNNNNNGC 2 cut(s) 38, 235
NciI CCSGG 1 cut(s) 555
NdeI CATATG 1 cut(s) 154
NdeII GATC 2 cut(s) 451, 489
NlaIII CATG 4 cut(s) 257, 300, 336, 625
NlaIV GGNNCC 5 cut(s) 7, 453, 476, 552, 613
NmuCI GTSAC 2 cut(s) 349, 662
NspV TTCGAA 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 492
PflMI CCANNNNNTGG 1 cut(s) 101
PfoI TCCNGGA 1 cut(s) 447
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 4 cut(s) 244, 542, 599, 634
PpsI GAGTC 4 cut(s) 244, 542, 599, 634
PshAI GACNNNNGTC 1 cut(s) 443
Psp6I CCWGG 1 cut(s) 447
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 5 cut(s) 7, 453, 476, 552, 613
PstI CTGCAG 1 cut(s) 445
PsuI RGATCY 1 cut(s) 451
PvuII CAGCTG 1 cut(s) 78
RsaI GTAC 2 cut(s) 330, 421
RsaNI GTAC 2 cut(s) 329, 420
RseI CAYNNNNRTG 2 cut(s) 301, 395
SaqAI TTAA 3 cut(s) 122, 483, 645
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 2 cut(s) 451, 489
SchI GAGTC 4 cut(s) 244, 543, 600, 635
ScrFI CCNGG 2 cut(s) 449, 555
SetI ASST 8 cut(s) 80, 162, 185, 207, 489, 501, 522, 651
SfcI CTRYAG 3 cut(s) 178, 433, 441
SfuI TTCGAA 1 cut(s) 312
SmiMI CAYNNNNRTG 2 cut(s) 301, 395
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 2 cut(s) 133, 308
SsiI CCGC 1 cut(s) 588
SspMI CTAG 2 cut(s) 110, 667
StyD4I CCNGG 2 cut(s) 447, 553
TaiI ACGT 2 cut(s) 489, 501
TaqI TCGA 5 cut(s) 71, 259, 312, 528, 559
TasI AATT 2 cut(s) 133, 308
Tru1I TTAA 3 cut(s) 122, 483, 645
Tru9I TTAA 3 cut(s) 122, 483, 645
TseFI GTSAC 2 cut(s) 349, 662
TseI GCWGC 1 cut(s) 75
Tsp45I GTSAC 2 cut(s) 349, 662
TspDTI ATGAA 3 cut(s) 175, 285, 563
Van91I CCANNNNNTGG 1 cut(s) 101
XmiI GTMKAC 3 cut(s) 213, 501, 594
XspI CTAG 2 cut(s) 110, 667
ZraI GACGTC 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.