MD10G1031300.v1.1

Citrate-binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000532
Physical Location & Seq
Forward (+)
4047345 .. 4048352
1008 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1031300.v1.1.491

Sequence Viewer

Length: 393 bp
ATGAGGGGATTTGACTACTCATCTGGAATATGGCAATTTGAAGGGTATGGATTTGTGCCAAATGGAACCTCTGGTGCTACAGTAGCACAGATCCATGGAGCAGCTAAGGGTGCTACGACTATAATCCTAAGAATCTATAACGGCGACATGAGGTATTATAGTCGAGATTTGGTGGGTACAAATCTTTACGATAAGTGGTTCAGACTTAACATAATCCATGACGTCGACGGAGGGAGCGTGACTATTTTCATTGACGGAGTCCAGAAATTTCGGGTGAAGGATCAAGGGCCAGGAGATTTGTACTTCAAATGTGGAGTTTATGCTGCACCGGCTAACATTACCTACTACATGGAATCACGTTGGAAAGACATTGAAATATATAAAAAGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Alginate_lyase2

Protein Analysis

131

Amino Acids

14.8

Weight (kDa)

8.59

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alginate_lyase2 PF08787 6 - 127 5.3e-16 Alginate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000532)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g23820 FvH4_1g23821 FvH4_1g23860 FvH4_1g23860 FvH4_2g13510 FvH4_2g13513 FvH4_2g13520 FvH4_2g13550
malus_domestica MD05G1029900.v1.1 MD07G1183700.v1.1 MD10G1027700.v1.1 MD10G1027900.v1.1 MD10G1028300.v1.1 MD10G1030500.v1.1 MD10G1031000.v1.1 MD10G1031300.v1.1 MD15G1341000.v1.1
prunus_persica Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.6G161900_v2.0.a1 Prupe.8G032800_v2.0.a1 Prupe.8G032900_v2.0.a1 Prupe.8G033100_v2.0.a1
pyrus_communis pycom01g03170
rosa_chinensis RchiOBHm_Chr2g0119701 RchiOBHm_Chr2g0119711 RchiOBHm_Chr2g0119741 RchiOBHm_Chr2g0119771 RchiOBHm_Chr2g0119811 RchiOBHm_Chr6g0275141 RchiOBHm_Chr6g0275151 RchiOBHm_Chr6g0275161
rosa_laevigata RLG00000013491 RLG00000013494 RLG00000018485 RLG00000018486 RLG00000018489 RLG00000018492
rosa_multiflora Rmu_sc0001501.1_g000054 Rmu_sc0001501.1_g000061 Rmu_sc0001501.1_g000088 Rmu_sc0006012.1_g000002 Rmu_sc0020284.1_g000003 Rmu_sc0033232.1_g000001 Rmu_sc0033428.1_g000001 Rmu_ssc0000041.1_g000008 Rmu_ssc0000041.1_g000015
rosa_roxburghii Rroxscaffold_2G00081650 Rroxscaffold_2G00124090 Rroxscaffold_2G00124110 Rroxscaffold_7G00193590 Rroxscaffold_7G00193610
rosa_rugosa Rorug02G0223300 Rorug02G0223500 Rorug06G0088700 Rorug06G0088800 Rorug06G0088900 Rorug06G0089000 Rorug06G0089100
rosa_samantha Rh2AG280400 Rh2AG280700 Rh2AG280800 Rh2BG292200 Rh2BG292500 Rh2BG292800 Rh2DG287400 Rh2DG287600 Rh2DG306100 Rh2DG306500 Rh2DG306700 Rh6AG202700 Rh6AG202900 Rh6AG203100 Rh6BG205900 Rh6CG208000 Rh6CG208100 Rh6CG208300 Rh6DG198300 Rh6DG198400
rosa_wichuraiana Rw0G003770 Rw2G022460 Rw2G022490 Rw6G017600 Rw6G017610 Rw6G017620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 225
AccI GTMKAC 1 cut(s) 225
AclWI GGATC 2 cut(s) 85, 288
AcsI RAATTY 1 cut(s) 266
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 3 cut(s) 178, 302, 389
AgsI TTSAA 3 cut(s) 41, 307, 374
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 1 cut(s) 104
AluI AGCT 1 cut(s) 104
AlwI GGATC 2 cut(s) 85, 288
AoxI GGCC 1 cut(s) 287
ApeKI GCWGC 2 cut(s) 101, 323
ApoI RAATTY 1 cut(s) 266
AspS9I GGNCC 1 cut(s) 287
AsuHPI GGTGA 1 cut(s) 286
BbvI GCAGC 2 cut(s) 113, 310
BceAI ACGGC 1 cut(s) 157
BciT130I CCWGG 1 cut(s) 291
BfmI CTRYAG 1 cut(s) 78
BisI GCNGC 2 cut(s) 102, 324
BlsI GCNGC 2 cut(s) 103, 325
BmcAI AGTACT 1 cut(s) 389
Bme1390I CCNGG 1 cut(s) 291
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 67
BmrFI CCNGG 1 cut(s) 291
Bpu10I CCTNAGC 1 cut(s) 105
BsaHI GRCGYC 1 cut(s) 222
BsaJI CCNNGG 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 222, 252
Bse118I RCCGGY 1 cut(s) 328
BseBI CCWGG 1 cut(s) 291
BseDI CCNNGG 1 cut(s) 94
BseXI GCAGC 2 cut(s) 113, 310
BsgI GTGCAG 1 cut(s) 309
BshFI GGCC 1 cut(s) 289
BsiSI CCGG 1 cut(s) 329
BsnI GGCC 1 cut(s) 289
Bsp143I GATC 2 cut(s) 90, 280
Bsp19I CCATGG 1 cut(s) 94
BspANI GGCC 1 cut(s) 289
BspLI GGNNCC 1 cut(s) 67
BspPI GGATC 2 cut(s) 85, 288
BsrFI RCCGGY 1 cut(s) 328
BssAI RCCGGY 1 cut(s) 328
BssECI CCNNGG 1 cut(s) 94
BssMI GATC 2 cut(s) 90, 280
BssNI GRCGYC 1 cut(s) 222
BssT1I CCWWGG 1 cut(s) 94
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 222
BstDEI CTNAG 2 cut(s) 105, 128
BstDSI CCRYGG 1 cut(s) 94
BstKTI GATC 2 cut(s) 93, 283
BstMBI GATC 2 cut(s) 90, 280
BstMWI GCNNNNNNNGC 3 cut(s) 83, 110, 329
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstSFI CTRYAG 1 cut(s) 78
BstV1I GCAGC 2 cut(s) 113, 310
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BsuRI GGCC 1 cut(s) 289
BtgI CCRYGG 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 328
Cfr13I GGNCC 1 cut(s) 287
Csp6I GTAC 3 cut(s) 177, 301, 388
CviAII CATG 4 cut(s) 95, 148, 218, 349
CviJI RGCY 3 cut(s) 104, 289, 332
CviKI_1 RGCY 3 cut(s) 104, 289, 332
CviQI GTAC 3 cut(s) 177, 301, 388
DdeI CTNAG 2 cut(s) 105, 128
DpnI GATC 2 cut(s) 92, 282
DpnII GATC 2 cut(s) 90, 280
Eco130I CCWWGG 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 289
EcoT14I CCWWGG 1 cut(s) 94
ErhI CCWWGG 1 cut(s) 94
FaeI CATG 4 cut(s) 98, 151, 221, 352
FatI CATG 4 cut(s) 94, 147, 217, 348
FblI GTMKAC 1 cut(s) 225
Fnu4HI GCNGC 2 cut(s) 102, 324
Fsp4HI GCNGC 2 cut(s) 102, 324
GluI GCNGC 2 cut(s) 102, 324
HaeIII GGCC 1 cut(s) 289
HapII CCGG 1 cut(s) 329
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 4 cut(s) 98, 151, 221, 352
HincII GTYRAC 1 cut(s) 226
HindII GTYRAC 1 cut(s) 226
HinfI GANTC 3 cut(s) 132, 258, 353
HpaII CCGG 1 cut(s) 329
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 1 cut(s) 203
Hpy188III TCNNGA 3 cut(s) 24, 164, 262
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 2 cut(s) 227, 230
HpyAV CCTTC 2 cut(s) 35, 271
HpyCH4III ACNGT 1 cut(s) 82
HpyCH4IV ACGT 2 cut(s) 222, 358
HpyCH4V TGCA 1 cut(s) 326
HpyF10VI GCNNNNNNNGC 3 cut(s) 83, 110, 329
HpyF3I CTNAG 2 cut(s) 105, 128
HpySE526I ACGT 2 cut(s) 222, 358
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 4 cut(s) 98, 151, 221, 352
Kzo9I GATC 2 cut(s) 90, 280
LmnI GCTCC 2 cut(s) 98, 234
LpnPI CCDG 6 cut(s) 9, 57, 275, 276, 303, 342
Lsp1109I GCAGC 2 cut(s) 113, 310
MaeII ACGT 2 cut(s) 222, 358
MaeIII GTNAC 1 cut(s) 238
MalI GATC 2 cut(s) 92, 282
MboI GATC 2 cut(s) 90, 280
MflI RGATCY 1 cut(s) 90
MluCI AATT 2 cut(s) 35, 266
MlyI GAGTC 1 cut(s) 267
MmeI TCCRAC 1 cut(s) 341
MnlI CCTC 3 cut(s) 79, 144, 224
MseI TTAA 1 cut(s) 207
MspI CCGG 1 cut(s) 329
MspR9I CCNGG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 83, 110, 329
NcoI CCATGG 1 cut(s) 94
NdeII GATC 2 cut(s) 90, 280
NlaIII CATG 4 cut(s) 98, 151, 221, 352
NlaIV GGNNCC 1 cut(s) 67
NmuCI GTSAC 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PfeI GAWTC 2 cut(s) 132, 353
PflFI GACNNNGTC 1 cut(s) 257
PkrI GCNGC 2 cut(s) 103, 325
PleI GAGTC 1 cut(s) 266
PpsI GAGTC 1 cut(s) 266
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 67
PspPI GGNCC 1 cut(s) 287
PsuI RGATCY 1 cut(s) 90
PsyI GACNNNGTC 1 cut(s) 257
RsaI GTAC 3 cut(s) 178, 302, 389
RsaNI GTAC 3 cut(s) 177, 301, 388
SalI GTCGAC 1 cut(s) 224
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 2 cut(s) 102, 324
Sau3AI GATC 2 cut(s) 90, 280
Sau96I GGNCC 1 cut(s) 287
ScaI AGTACT 1 cut(s) 389
SchI GAGTC 1 cut(s) 267
ScrFI CCNGG 1 cut(s) 291
SetI ASST 6 cut(s) 71, 106, 155, 225, 344, 361
SfcI CTRYAG 1 cut(s) 78
SgrDI CGTCGACG 1 cut(s) 224
Sse9I AATT 2 cut(s) 35, 266
StyD4I CCNGG 1 cut(s) 289
StyI CCWWGG 1 cut(s) 94
TaaI ACNGT 1 cut(s) 82
TaiI ACGT 2 cut(s) 225, 361
TaqI TCGA 2 cut(s) 163, 225
TasI AATT 2 cut(s) 35, 266
TatI WGTACW 2 cut(s) 300, 387
TfiI GAWTC 2 cut(s) 132, 353
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 2 cut(s) 101, 323
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 1 cut(s) 238
TspGWI ACGGA 2 cut(s) 243, 270
Tth111I GACNNNGTC 1 cut(s) 257
XapI RAATTY 1 cut(s) 266
XmiI GTMKAC 1 cut(s) 225
ZraI GACGTC 1 cut(s) 223
ZrmI AGTACT 1 cut(s) 389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.